Abstract: A simple process for producing ?-coelenterazine compounds has been desired. Described is a process for producing a ?-coelenterazine compound represented by general formula (II) comprising (1) the step of reacting a compound of general formula (VIII) with a methyltriphenylphosphonium salt in the presence of a base to give a compound represented by general formula (IX), (2) the step of performing a ring-closing metathesis reaction on any one selected from the group consisting of the compound represented by general formula (IX) and a compound of general formula (X) which is the compound of general formula (IX) wherein the amino is protected with R5, and then deprotecting R4 and, if any, R5 to give a ?-coelenteramine compound represented by general formula (XIV), and (3) the step of reacting the compound of general formula (XIV) with a compound represented by general formula (XV) to give the compound of general formula (II).

Abstract: This steel for a machine structure contains, in mass %: C: 0.40% to less than 0.75%; Si: 0.01% to 3.0%; Mn: 0.1% to 1.8%; S: 0.001% to 0.1%; Al: more than 0.1% and not more than 1.0%; N: 0.001% to 0.02%; and P: limited to not more than 0.05%, with a balance including Fe and inevitable impurities, in which the steel satisfies 139.38?214×[C]+30.6×[Si]+42.8×[Mn]?14.7×[Al]?177 and 0.72?[C]+1/7×[Si]+1/5×[Mn]<1.539.

Abstract: Provided is a screening method with which it is possible to determine the cause of a decrease or an increase in the amount of a target protein that is expressed by a cell. One or a plurality of embodiments include: conducting cultivation that includes bringing an assay cell into contact with a test substance, and measuring a relative amount (A) of the target protein in relation to an internal standard; conducting cultivation in which an assay cell is not brought into contact with a test substance, and measuring a relative amount (B) in relation to an internal standard; comparing the relative amount (A) and the relative amount (B); and, based on the comparison, selecting a candidate substance that induces instability and/or stability in the target protein.

Abstract: A steel material superior in high temperature characteristics and toughness is provided, that is, a steel material containing, by mass %, C: 0.005% to 0.03%, Si: 0.05% to 0.40%, Mn: 0.40% to 1.70%, Nb: 0.02% to 0.25%, Ti: 0.005% to 0.025%, N: 0.0008% to 0.0045%, B: 0.0003% to 0.0030%, restricting P: 0.030% or less, S: 0.020% or less, Al: 0.03% or less, and having a balance of Fe and unavoidable impurities, where the contents of C and Nb satisfy C—Nb/7.74?0.02 and Ti-based oxides of a grain size of 0.05 to 10 ?m are present in a density of 30 to 300/mm2.

Abstract: A simple process for producing ?-coelenterazine compounds has been desired. The invention provides a process for producing a ?-coelenterazine compound represented by general formula (II) which comprises (1) the step of reacting a compound represented by general formula (VIII) with a methyltriphenylphosphonium salt in the presence of a base to give a compound represented by general formula (IX), (2) the step of performing a ring-closing metathesis reaction on any one selected from the group consisting of the compound represented by general formula (IX) and a compound represented by general formula (X) which is the compound represented by general formula (IX) wherein the amino is protected with R5, and then deprotecting R4 and, if any, R5 to give a ?-coelenteramine compound represented by general formula (XIV), and (3) the step of reacting the compound represented by general formula (XIV) with a compound represented by general formula (XV) to give the compound represented by general formula (II).

Abstract: A simple process for producing v-coelenterazine compounds has been desired. Described is a process for producing a v-coelenterazine compound represented by general formula (II) comprising (1) the step of reacting a compound of general formula (VIII) with a methyltriphenylphosphonium salt in the presence of a base to give a compound represented by general formula (IX), (2) the step of performing a ring-closing metathesis reaction on any one selected from the group consisting of the compound represented by general formula (IX) and a compound of general formula (X) which is the compound of general formula (IX) wherein the amino is protected with R5, and then deprotecting R4 and, if any, R5 to give a v-coelenteramine compound represented by general formula (XIV), and (3) the step of reacting the compound of general formula (XIV) with a compound represented by general formula (XV) to give the compound of general formula (II).

Abstract: It has been found that efficient screening for a compound having anticancer activity can be achieved by selecting a compound having activity to induce alternative autophagy using, as an index, formation of fluorescent bright spots due to aggregation of a lysosomal protein to which a fluorescent protein is attached in cells expressing the lysosomal protein.

Abstract: The invention aims in establishing a method for modifying biomolecules using a reaction that efficiently modifies biomolecules and is widely applicable. The invention thus provides a cyclic compound containing two triazole rings formed by adding and ligating an azide compound possessing an azido group to each of the two carbon-carbon triple bond sites of an eight-membered cyclic skeleton of a cyclic diyne compound by a double click reaction; a method for producing a cyclic compound using a double click reaction; and a method for modifying biomolecules.

Abstract: The invention aims in establishing a method for modifying biomolecules using a reaction that efficiently modifies biomolecules and is widely applicable. The invention thus provides a cyclic compound containing two triazole rings formed by adding and ligating an azide compound possessing an azido group to each of the two carbon-carbon triple bond sites of an eight-membered cyclic skeleton of a cyclic diyne compound by a double click reaction; a method for producing a cyclic compound using a double click reaction; and a method for modifying biomolecules.

Abstract: A simple process for producing v-coelenterazine compounds has been desired. Described is a process for producing a v-coelenterazine compound represented by general formula (II) comprising (1) the step of reacting a compound of general formula (VIII) with a methyltriphenylphosphonium salt in the presence of a base to give a compound represented by general formula (IX), (2) the step of performing a ring-closing metathesis reaction on any one selected from the group consisting of the compound represented by general formula (IX) and a compound of general formula (X) which is the compound of general formula (IX) wherein the amino is protected with R5, and then deprotecting R4 and, if any, R5 to give a v-coelenteramine compound represented by general formula (XIV), and (3) the step of reacting the compound of general formula (XIV) with a compound represented by general formula (XV) to give the compound of general formula (II).

Abstract: Disclosed is a lip seal for water pump made of a rubber-like elastic material, fixed to a housing as a fixed side and in sliding contact with a shaft rotating relative to the housing; the lip seal being obtained by vulcanization-molding of a rubber composition comprising 100 parts by weight of the rubber-like elastic material, 1 to 150 parts by weight of a reinforcing filler, 5 to 90 parts by weight of a non-reinforcing filler having an average particle diameter of 1 ?m or more, 0.1 to 5 parts by weight of a coupling agent, 1 to 15 parts by weight of a co-crosslinking agent, and 0.5 to 10 parts by weight of an organic peroxide. The lip seal for water pump provides a materially solution, rather than a structural solution, to prevent softening and volume swelling of the rubber-like elastic material and the generation of deposits in the rotating shaft, which are problematic for rotation torque and LLC resistance.

Abstract: A simple process for producing v-coelenterazine compounds has been desired. Described is a process for producing a v-coelenterazine compound represented by general formula (II) comprising (1) the step of reacting a compound of general formula (VIII) with a methyltriphenylphosphonium salt in the presence of a base to give a compound represented by general formula (IX), (2) the step of performing a ring-closing metathesis reaction on any one selected from the group consisting of the compound represented by general formula (IX) and a compound of general formula (X) which is the compound of general formula (IX) wherein the amino is protected with R5, and then deprotecting R4 and, if any, R5 to give a v-coelenteramine compound represented by general formula (XIV), and (3) the step of reacting the compound of general formula (XIV) with a compound represented by general formula (XV) to give the compound of general formula (II).

Abstract: This high-carbon pearlitic steel rail having excellent ductility, includes: in terms of percent by mass, C: more than 0.85% to 1.40%; Si: 0.10% to 2.00%; Mn: 0.10% to 2.00%; Ti: 0.001% to 0.01%; V: 0.005% to 0.20%; and N: less than 0.0040%, with the balance being Fe and inevitable impurities, wherein contents of Ti and V fulfill the following formula (1), and a rail head portion has a pearlite structure.

Abstract: The present invention provides high temperature strength and fire-resistant steel superior in weld joint reheat embrittlement resistance and toughness which is produced using steel of a room temperature strength of the 400 to 600N/mm2 class containing as main ingredients C: 0.010% to less than 0.05%, Si: 0.01 to 0.50%, Mn: 0.80 to 2.00%, Cr: 0.50% to less than 2.00%, V: 0.03 to 0.30%, Nb: 0.01 to 0.10%, N: 0.001 to 0.010%, and Al: 0.005 to 0.10%, limiting the contents of Ni, Cu, Mo, and B, and satisfying the relationship of 4Cr[%]-5Mo[%]-10Ni[%]-2Cu[%]-Mn[%]>0.

Abstract: The cutting method of steel for machine structural use includes: forming a mist by mixing a cutting fluid supplied at a supply rate of 0.01 ml/hour to 200 ml/hour and an oxidizing gas containing, by volume %, 21% to 50% of oxygen; and cutting the steel for machine structural use while the mist is blown on the cutting edge surface of a tool and the surface of the steel for machine structural use, wherein the steel for machine structural use includes, by mass %: C: 0.01% to 1.2%, Si: 0.005% to 3.0%, Mn: 0.05% to 3.0%, P: 0.001% to 0.2%, S: 0.001% to 0.35%, N: 0.002% to 0.035%, Al: 0.05% to 1.0%, and the balance consisting of Fe and inevitable impurities in which O is limited to 0.003% or less, and the amount of Al [Al %] and the amount of N [N %] satisfy [Al %]?(27/14)×[N %]?0.05.

Abstract: In the method of cutting steel for machine structural use, steel for machine structural use, in which chemical components contain, by mass %, 0.01% to 1.2% of C, 0.005% to 3.0% of Si, 0.05% to 3.0% of Mn, 0.001% to 0.2% of P, 0.001% to 0.35% of S, 0.002% to 0.035% of N and 0.05% to 1.0% of Al, in a case in which [Al%] and [N%] represent the mass % of Al and N in the steel respectively, [Al%]?(27/14)×[N%]?0.05% is satisfied, is the other elements are included as necessary, and a balance consisting of Fe and inevitable impurities, is used, and a cutting process is carried out on the steel for machine structural use using water-insoluble cutting oil in which the amount of dissolved oxygen is 4 volume % to 16 volume %.

Abstract: A steel for surface hardening for machine structural use which is characterized by containing, in terms of mass %, 0.30-0.60% C, 0.02-2.0% Si, 0.35-1.5% Mn, 0.001-0.5% Al, 0.05-2.0% Cr, 0.001-1.0% Sn, 0.0001-0.021% S, 0.0030-0.0055% N, 0.01-2.0% Ni, 0.01-2.0% Cu, up to 0.030% P, and up to 0.005% O, with the remainder comprising Fe and incidental impurities, the contents of Sn, Cu, Ni, Mn, and S satisfying relationships (1) and (2). ?0.19?0.12×Sn+Cu?0.1×Ni?0.

Abstract: This steel for a machine structure contains, in mass %: C: 0.40% to less than 0.75%; Si: 0.01% to 3.0%; Mn: 0.1% to 1.8%; S: 0.001% to 0.1%; Al: more than 0.1% and not more than 1.0%; N: 0.001% to 0.02%; and P: limited to not more than 0.05%, with a balance including Fe and inevitable impurities, in which the steel satisfies 139.38 ?214×[C]+30.6×[Si]+42.8×[Mn]?14.7×[Al]?177 and 0.72?[C]+1/7×[Si]+1/5×[Mn]<1.539.

Abstract: The invention aims in establishing a method for modifying biomolecules using a reaction that efficiently modifies biomolecules and is widely applicable. The invention thus provides a cyclic compound containing two triazole rings formed by adding and ligating an azide compound possessing an azido group to each of the two carbon-carbon triple bond sites of an eight-membered cyclic skeleton of a cyclic diyne compound by a double click reaction; a method for producing a cyclic compound using a double click reaction; and a method for modifying biomolecules.

Abstract: The present invention provides a fire-resistant steel material superior in HAZ toughness of a welded joint which is high in high temperature yield strength at an envisioned fire temperature of 700 to 800° C. and is free of embrittlement of the welded joint even if exposed at this envisioned fire temperature and a method of production of the same, that is, a fire-resistant steel material of a composition containing, by mass %, C: 0.005% to less than 0.03%, Si: 0.01 to 0.50%, Mn: 0.05 to 0.40%, Cr: 1.50 to 5.00%, V: 0.05 to 0.50%, and N: 0.001 to 0.005% and restricted in contents of Ni, Cu, Mo, B, P, S, and O obtained by heating a steel slab to 1150 to 1300° C., then hot working or hot rolling the slab to an end temperature of 880 degrees or more, acceleratedly cooling the worked or rolled steel material under conditions of a cooling rate at a position of the slowest cooling rate of at least 2° C.