Abstract: A car navigation system searches for a route from a start point to a destination, and provides guidance along the route. The car navigation system includes a trifurcate guidance section that makes a decision of considering two consecutive bifurcate guidance nodes among guidance nodes stored in a guidance node storing section as a single trifurcate guidance node, and makes a decision of a guidance direction to be provided as trifurcate guidance. The trifurcate guidance section provides the trifurcate guidance at the single trifurcate guidance node via a guidance section. The car navigation system can provide the guidance indicating a driver the way to proceed plainly, when two successive bifurcate intersections are present in a short distance.

Abstract: The present invention is to provide a curable coating composition having much less of a volatile organic component, and being excellent in coating film performance such as durability and scratch resistance, and a coating film forming method using the above curable coating composition capable of suppressing generation of sagging and so on.

Abstract: The disclosed invention provides methods and instruments for fluorescence detection making it possible to separate and detect analytes of a plurality of species in a migration (separation) channel with length of the order of millimeters. Analyte samples disperse across the whole detection region of a migration channel filled with a sieving matrix. Electrodes located in contact with a power supply and the sieving matrix cause the analytes to electophoretically migrate at predetermined velocity V. The detection region is irradiated by excitation light whose intensity changes in a cycle equaling pitch p in the direction that the analytes move. Fluorescence emission from the analytes exposed to the excitation light is detected by a detector. Fluctuation &dgr;i (t) of output current from the detector is analyzed by a spectrum analyzer and the obtained spectrum is displayed.

Abstract: A vehicle navigation system includes a road-between-guidance-target-points configuration determining unit (9) for determining a configuration of a road between two successive guidance target points whose information is extracted from information stored in a guidance target point storing unit (8). A continuous guidance threshold distance setting unit (10) sets a threshold distance for continuous guidance based on the road configuration by using a table or the like. A distance-between-guidance-target-points calculating unit (11) calculates a distance between the two successive guidance target points. When the calculated distance between the two successive guidance target points is shorter than the set threshold distance for continuous guidance, a route guidance unit (12) provides continuous guidance for users through a guidance unit (16).

Abstract: Adenylation of a DNA fragment with a DNA polymerase occurs in the course of PCR, and thus two peaks are detected. To prevent the peak splitting, it is necessary to raise efficiency of adenylation a single peak to occur without changing reaction conditions. To this end, four types of PCR primers which, respectively, have an anchor sequence at 5′ terminus so that any of A, C, G or T is attached to at the 5′ terminus of the anchor sequence, and PCR is carried out by use of the respective primers to determine efficiencies of adenylation. Subsequently, an anchor sequence that is more likely to undergo adenylation is screened to decide an anchor sequence more likely undergo adenylation, followed by PCR by use of a primer having the decided anchor sequence.

Abstract: A car navigation system searches for a route from a start point to a destination, and provides guidance along the route. The car navigation system includes a trifurcate guidance section that makes a decision of considering two consecutive bifurcate guidance nodes among guidance nodes stored in a guidance node storing section as a single trifurcate guidance node, and makes a decision of a guidance direction to be provided as trifurcate guidance. The trifurcate guidance section provides the trifurcate guidance at the single trifurcate guidance node via a guidance section. The car navigation system can provide the guidance indicating a driver the way to proceed plainly, when two successive bifurcate intersections are present in a short distance.

Abstract: A system and method are provided for fraction collection for capillary electrophoresis. In one example, the system includes a capillary configured to electrophoretically separate biological fractions into separated biological fractions, and further configured to discharge the separated biological fractions from a capillary end; a flow cell surrounding the capillary end, wherein the flow cell is configured to collect the separated biological fractions discharged from the capillary end into a collecting vessel; an irradiator configured to irradiate a light beam onto the capillary at a detection point, wherein the light beam causes emitted light from the capillary; and a detector configured to detect the emitted light.

Abstract: When one's vehicle goes into a road from outside the road, the direction of turning is indicated by guiding.

Abstract: A capillary electrophoresis system includes a plurality of capillaries arranged in parallel on a vertical plane at an identical distance from each other, each of which has a sample injection end immersed in a cathode buffer 23 and pointed downward vertically to facilitate connection between the cathode buffer 23 and a sample solution. The sample elution end is connected via a pump block 11 and a connection tube 3 to an anode buffer 24. The height is made identical between the liquid levels for buffers 23 and 24 to prevent movement of the separation medium in the capillaries. The sample introduced from the sample injection end 20 moves upward vertically via electrophoresis to the sample elution end in the capillary and is passed through and detected at the fluorescence detection position 19. The effective separation length is thus decreased to 10 cm or less to attain high throughput.

Abstract: A capillary electrophoresis system includes a plurality of capillaries arranged in parallel on a vertical plane at an identical distance from each other, each of which has a sample injection end immersed in a cathode buffer 23 and pointed downward vertically to facilitate connection between the cathode buffer 23 and a sample solution. The sample elution end is connected via a pump block 11 and a connection tube 3 to an anode buffer 24. The height is made identical between the liquid levels for buffers 23 and 24 to prevent movement of the separation medium in the capillaries. The sample introduced from the sample injection end 20 moves upward vertically via electrophoresis to the sample elution end in the capillary and is passed through and detected at the fluorescence detection position 19. The effective separation length is thus decreased to 10 cm or less to attain high throughput.

Abstract: A link that is closest to a departure place of one's vehicle is detected by a route searching. When a U-turn route link to come back from the closest link by passing from the closest link through the U-turn route again to the closest link is detected, a U-turn guide is made, based on a signal of this detection, to the effect that a return is made to the present position after a U-turn.

Abstract: A suspension insulator having a shed including an upper surface and an under surface has a pin fitting arranged at its center portion and projected from the under surface and a plurality of circular ribs arranged around the pin fitting in a concentric manner. In a first aspect of the invention, a resistance zone having a surface resistance (1 cm×1 cm) of not greater than 4 M&OHgr; and arranged on the under surface at an inner portion continuing from the pin fitting and a conductive zone arranged on the under surface at an outer peripheral portion of the resistance zone. In a second aspect of the invention, a resistance zone arranged on the under surface at an inner portion continuing from the pin fitting and existing between the pin fitting and an inner root portion of the rib.

Abstract: Adenylation of a DNA fragment with a DNA polymerase occurs in the course of PCR, and thus two peaks are detected. To prevent the peak splitting, it is necessary to raise efficiency of adenulation a single peak to occur without changing reaction conditions. To this end, four types of PCR primers which, respectively, have an anchor sequence at 5′ terminus so that any of A, C, G or T is attached to at the 5′ terminus of the anchor sequence, and PCR is carried out by use of the respective primers to determine efficiencies of adenylation. Subsequently, an anchor sequence that is more likely to undergo adenylation is screened to decide an anchor sequence more likely undergo adenylation, followed by PCR by use of a primer having the decided anchor sequence.

Abstract: The disclosed invention provides methods and instruments for fluorescence detection making it possible to separate and detect analytes of a plurality of species in a migration (separation) channel with length of the order of millimeters. Analyte samples disperse across the whole detection region of a migration channel filled with a sieving matrix. Electrodes located in contact with a power supply and the sieving matrix cause the analytes to electophoretically migrate at predetermined velocity V. The detection region is irradiated by excitation light whose intensity changes in a cycle equaling pitch p in the direction that the analytes move. Fluorescence emission from the analytes exposed to the excitation light is detected by a detector. Fluctuation &dgr;i (t) of output current from the detector is analyzed by a spectrum analyzer and the obtained spectrum is displayed.

Abstract: The present invention provides a thermosetting coating composition having a storage stability sufficient to be formed into a one-pack coating despite a small content of organic solvent contained therein and having a sufficient curability, and a method of forming a coating film by which the content of the organic solvent present in a coating at the time of application can be reduced.

Abstract: There are beforehand prepared a monomer having a reaction residue and a polynucleotide probe set comprising plural kinds of polynucleotide probes having a residue bonded to the reaction residue. The monomer is mixed with each kind of polynucleotide probes comprising any plural probes selected from the polynucleotide probe set. Each kind of the resultant mixtures is added to each of different small holes to make the mixture into gel matrix. Thus, a polynucleotide probe chip is produced. Sample DNA is forcibly migrated in the gels by electrophoresis. Laser light is projected onto the side face of the chip. The fluorescence emitted from the whole surface of the chip is collectively detected with a high-sensitive two-dimensional detector. Thus, the polynucleotide probe chip, holding various kinds of DNA probes, for detecting DNA can be provided. This chip has high hybridization-efficiency and makes high-sensitivity and high-speed DNA detection possible.

Abstract: The present invention is to provide a curable coating composition having much less of a volatile organic component, and being excellent in coating film performance such as durability and scratch resistance, and a coating film forming method using the above curable coating composition capable of suppressing generation of sagging and so on.

Abstract: Focusing that abundant class genes abundantly present in a nucleic acid sample can comparatively easily be analyzed and readily be removed in a selective manner, a nucleic acid sample for expression analysis of rare expressed genes can be obtained by removing abundant genes therefrom, and analyses based upon the sample can be achieved.

Abstract: Focusing that abundant class genes abundantly present in a nucleic acid sample can comparatively easily be analyzed and readily be removed in a selective manner, a nucleic acid sample for expression analysis of rare expressed genes can be obtained by removing abundant genes therefrom, and analyses based upon the sample can be achieved.

Abstract: A suspension insulator having a shed including an upper surface and an under surface has a pin fitting arranged at its center portion and projected from the under surface and a plurality of circular ribs arranged around the pin fitting in a concentric manner. In a first aspect of the invention, a resistance zone having a surface resistance (1 cm×1 cm) of not greater than 4 M&OHgr; and arranged on the under surface at an inner portion continuing from the pin fitting and a conductive zone arranged on the under surface at an outer peripheral portion of the resistance zone. In a second aspect of the invention, a resistance zone arranged on the under surface at an inner portion continuing from the pin fitting and existing between the pin fitting and an inner root portion of the rib.