Patents by Inventor Tiemo Anhut
Tiemo Anhut has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10527834Abstract: A functionally integrated laser scanning microscope for scanning a sample with laser illumination, selectably in a confocal, line or wide-field operating mode, comprising a laser light source, an illumination and detection beam path, a detection device and at least one objective, wherein the illumination and detection beam path has optical means for the configuration of the laser illumination, at least one scanner for scanning the sample with the laser illumination, and a beam splitter for separating illumination and detection light, and controllable optical elements for changing the beam guiding depending on the operating mode selected in each case.Type: GrantFiled: November 6, 2018Date of Patent: January 7, 2020Assignees: Carl Zeiss Microscopy GmbH, Carl Zeiss AGInventors: Daniel Schwedt, Matthias Wald, Tiemo Anhut
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Publication number: 20190331903Abstract: The invention relates to an optical arrangement, particularly for the detection beam path of a multi-spot scanning microscope, comprising a detection plane, in which a detector is positionable, comprising a dispersive device for spectrally splitting detection light. According to the invention, the optical arrangement is characterized in that a distorting optical unit is present for guiding the detection light into the detection plane, said distorting optical unit being arranged downstream of the dispersive device and upstream of a detection plane, and in that a rotating device is present for the relative rotation of a luminous field of the spectrally separated detection light and the distorting optical unit. The invention additionally relates to a multi-spot scanning microscope and a method for operating a microscope.Type: ApplicationFiled: October 25, 2017Publication date: October 31, 2019Inventors: Matthias Wald, Tiemo Anhut, Daniel Schwedt
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Publication number: 20190265455Abstract: The invention relates to a beam manipulation device for a scanning microscope, comprising a main colour splitter for coupling excitation light into an illumination beam path and for separating excitation light from detection light of a detection beam path, said device comprising a scanner, preferably positioned on a pupil plane, for scanning the excitation light. The device is characterised in that: an additional optical section is provided comprising optical elements which influence a beam path; at least one pupil plane and/or at least one intermediate image plane is formed in the additional optical section by the optical elements which influence the beam path; and an adjustable selection device is provided for activating either a first beam segment of the illumination and/or detection beam path, or the additional optical section, wherein the first beam segment of the illumination and/or detection beam path does not contain a pupil plane of the illumination and/or detection beam path.Type: ApplicationFiled: October 16, 2017Publication date: August 29, 2019Inventors: Matthias Wald, Tiemo Anhut
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Publication number: 20190258041Abstract: The invention relates to an optical group for detection light of a microscope, in particular a confocal scanning microscope, having an input plane (10) for the passage of detection light to be measured and having a detection beam path arranged downstream of the input plane for guiding the detection light (11) into a detection plane (67), wherein the detection beam path has at least one first beam course (1) having first optical beam-guiding means, in particular first lenses and/or mirrors (20, 30, 34, 36, 58, 60, 66), for guiding the detection light into the detection plane. In the first beam course, the optical group has at least one dispersive device (26) for the spatial spectral splitting of the detection light to be measured and a manipulation device (49) for manipulating the spectrally spatially split detection light.Type: ApplicationFiled: October 16, 2017Publication date: August 22, 2019Inventors: Tiemo Anhut, Matthias Wald, Daniel Schwedt
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Patent number: 10371574Abstract: A detection device for a microscope comprises a dispersive element in the beam path of light and a selection element. The selection element separates a beam path of a spectral portion of the light from the beam path of the light. The detector device furthermore comprises a focusing optical unit configured to focus the beam path of the spectral portion of the light onto a sensor. By way of example, the microscope may be a confocal microscope.Type: GrantFiled: October 25, 2018Date of Patent: August 6, 2019Assignee: Carl Zeiss Microscopy GmbHInventors: David Shafer, Tiemo Anhut, Matthias Wald
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Publication number: 20190227290Abstract: A functionally integrated laser scanning microscope for scanning a sample with laser illumination, selectably in a confocal, line or wide-field operating mode, comprising a laser light source, an illumination and detection beam path, a detection device and at least one objective, wherein the illumination and detection beam path has optical means for the configuration of the laser illumination, at least one scanner for scanning the sample with the laser illumination, and a beam splitter for separating illumination and detection light, and controllable optical elements for changing the beam guiding depending on the operating mode selected in each case.Type: ApplicationFiled: November 6, 2018Publication date: July 25, 2019Applicants: Carl Zeiss Microscopy GmbH, Carl Zeiss AGInventors: Daniel SCHWEDT, Matthias WALD, Tiemo ANHUT
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Patent number: 10310243Abstract: The invention relates to a device for multispot scanning microscopy, having a multicolor light source for providing at least one illumination light beam, having a splitting device for splitting the illumination light beam into a plurality of illumination sub-beams, having first optical means for providing an illumination optical path for guiding and focusing the individual illumination sub-beams respectively into a light spot on or in a specimen to be examined, having a scan unit for guiding the light spots over the probe, having a detection unit for detecting detection light emitted by the specimen in detection sub-beams after irradiation with the individual illumination sub-beams, having second optical means for providing a detection optical path for guiding the detection sub-beams to the detector unit, having a control and evaluation unit for controlling the scan unit and for evaluating the detection light detected by the detection unit.Type: GrantFiled: February 8, 2017Date of Patent: June 4, 2019Assignee: Carl Zeiss Microscopy GmbHInventors: Tiemo Anhut, Daniel Schwedt, Tobias Kaufhold, Burkhard Roscher, Stefan Wilhelm
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Patent number: 10261300Abstract: The invention relates to a light microscope comprising a polychromatic light source for emitting illumination light in the direction of a sample, focussing means for focussing illumination light onto the sample, wherein the focussing means, for generating a depth resolution, have a longitudinal chromatic aberration, and a detection device, which comprises a two-dimensional array of detector elements, for detecting sample light coming from the sample. According to the invention, the light microscope is characterized in that, for detecting both confocal portions and non-confocal portions of the sample light, a beam path from the sample to the detection device is free of elements for completely masking out non-confocal portions. In addition, the invention relates to a method for image recording using a light microscope.Type: GrantFiled: May 16, 2013Date of Patent: April 16, 2019Assignee: Carl Zeiss Microscopy GmbHInventors: Helmut Lippert, Ralf Netz, Tiemo Anhut, Nils Langholz, Matthias Langhorst
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Patent number: 10254524Abstract: Beam deflection units in light-scanning microscopes are usually arranged in planes that are conjugate to the objective pupil. The scan optics, which is required for generating the conjugate pupil planes, is complicated and not very light efficient. The invention is intended to enable a higher image quality, simpler adjustment and a lower light loss microscope. The optical system comprises a concave mirror (36) for imaging a respective point of the first and second beam deflection units (30A, 30B) onto one another. The concave mirror (36), the first beam deflection unit (30A), and the second beam deflection unit (30B) are arranged such that the illumination beam path is reflected exactly once at the concave mirror (36). A first distortion caused by the concave mirror (36) and a second distortion of the imaging caused by the first and second beam deflection units (30A, 30B) at least partly compensate for one another.Type: GrantFiled: November 11, 2015Date of Patent: April 9, 2019Assignee: Carl Zeiss Microscopy GmbHInventors: Daniel Schwedt, Tiemo Anhut, Matthias Wald, Beate Böhme, Tobias Kaufhold
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Evaluation of signals of fluorescence scanning microscopy using a confocal laser scanning microscope
Patent number: 10234667Abstract: A method for evaluating signals of fluorescence scanning microscopy with simultaneous excitation and detection of fluorescence in different focal planes of a sample by means of confocal laser scanning microscopy.Type: GrantFiled: May 9, 2016Date of Patent: March 19, 2019Assignees: Carl Zeiss Microscopy GmbH, Carl Zeiss AGInventors: Yauheni Novikau, Thomas Kalkbrenner, Tiemo Anhut, Daniel Schwedt, Matthias Wald -
Publication number: 20190063993Abstract: A detection device for a microscope comprises a dispersive element in the beam path of light and a selection element. The selection element separates a beam path of a spectral portion of the light from the beam path of the light. The detector device furthermore comprises a focusing optical unit configured to focus the beam path of the spectral portion of the light onto a sensor. By way of example, the microscope may be a confocal microscope.Type: ApplicationFiled: October 25, 2018Publication date: February 28, 2019Inventors: David Shafer, Tiemo Anhut, Matthias Wald
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Patent number: 10203490Abstract: A functionally integrated laser scanning microscope for scanning a sample with laser illumination, selectably in a confocal, line or wide-field operating mode, comprising a laser light source, an illumination and detection beam path, a detection device and at least one objective, wherein the illumination and detection beam path has optical means for the configuration of the laser illumination, at least one scanner for scanning the sample with the laser illumination, and a beam splitter for separating illumination and detection light, and controllable optical elements for changing the beam guiding depending on the operating mode selected in each case.Type: GrantFiled: May 22, 2015Date of Patent: February 12, 2019Assignees: Carl Zeiss Microscopy GmbH, Carl Zeiss AGInventors: Daniel Schwedt, Matthias Wald, Tiemo Anhut
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Patent number: 10156710Abstract: A microscope with at least one illumination beam that is phase modulated in a section along its cross-section with a modulation frequency and a microscope lens for focusing the illumination beam into a test as well as a detection beam path and at least one means of demodulation, wherein at least one polarization altering item is scheduled in the illuminating beam path, for which a phase plate is subordinated that exhibits at least two areas with different phase influence.Type: GrantFiled: October 3, 2011Date of Patent: December 18, 2018Assignee: Carl Zeiss Microscopy GmbHInventors: Tiemo Anhut, Thomas Kalkbrenner, Daniel Schwedt
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Patent number: 10132685Abstract: A detection device for a microscope comprises a dispersive element in the beam path of light and a selection element. The selection element separates a beam path of a spectral portion of the light from the beam path of the light. The detector device furthermore comprises a focusing optical unit configured to focus the beam path of the spectral portion of the light onto a sensor. By way of example, the microscope may be a confocal microscope.Type: GrantFiled: November 5, 2015Date of Patent: November 20, 2018Assignee: Carl Zeiss Microscopy GmbHInventors: David Shafer, Tiemo Anhut, Matthias Wald
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Patent number: 10095017Abstract: A microscope, preferably a laser scanning microscope, with at least one illuminating beam, which in a partial area along the cross-section thereof, is phase-modulated with a modulation frequency. A microscope objective is provided for focusing the illumination beam onto a sample. The microscope further has a detection beam path and at least one demodulation means, wherein a pulsed illumination beam is present. In the illumination beam path upstream of the microscope objective, a first polarization beam splitter is provided, which generates at least first and second partial beam paths that have differing, preferably adjustable, optical paths. A combination element, such as a second pole splitter, for rejoining the partial beams is provided. In one partial beam path, a phase element is provided, which has at least two areas having differing phase interferences.Type: GrantFiled: May 7, 2013Date of Patent: October 9, 2018Assignee: Carl Zeiss Microscopy GmbHInventors: Tiemo Anhut, Thomas Kalkbrenner, Ralf Netz
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Patent number: 10031325Abstract: An arrangement for light sheet microscopy that includes a means for scanning a sample volume with a light sheet, which includes an angle ??90° with the optical axis of an objective. The light sheet passes through the entire sample volume in the propagation direction, and the depth of field Sobj of the objective is less than the optical-axis depth T of this sample volume. An optical device, disposed downstream of the objective, increases the depth of field Sobj to a depth of field Seff?the depth T of this sample volume. The arrangement also includes a means for positioning the sample volume within the region of the depth of field Seff. A spatially resolving optoelectronic area sensor is disposed downstream of the optical device, and hardware and software are provided to generate sample-volume images from the electronic image signals output by the area sensor.Type: GrantFiled: February 19, 2015Date of Patent: July 24, 2018Assignee: Carl Zeiss Microscopy GmbHInventors: Tiemo Anhut, Thomas Kalkbrenner, Daniel Schwedt, Jörg Siebenmorgen, Helmut Lippert
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Publication number: 20180174279Abstract: A method for the determination and compensation of geometric imaging errors which occur during the imaging of an object by sequential single or multispot scanning by means of a microscopic imaging system, which includes: establishing a reference object with a defined plane structure; generating an electronic image data set of this structure free of geometric imaging errors; generating at least one electronic actual image data set with the imaging system; comparing the actual image data set with the reference image data set with respect to the locations of those pixels which have the same object point as origin in each case; determining location deviations in the actual image data set compared to the reference image data set; saving determined location deviations as correction data; and compensating for the geometric imaging errors by correction of the location deviations in the actual image data set with reference to the correction data.Type: ApplicationFiled: June 3, 2016Publication date: June 21, 2018Inventors: Tiemo ANHUT, Tobias KAUFHOLD, Daniel SCHWEDT, Burkhard ROSCHER, Frank KLEMM, Daniel HAASE
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Patent number: 9989754Abstract: A light scanning microscope with an illumination module switchable between an illumination with m number of spots and an illumination with n number of spots, a deflecting unit which moves the m or n spots in a predetermined sample region, and a detector module for confocal and spectrally resolved detection of the sample radiation. The detector module has a confocal diaphragm unit, a splitting unit which is arranged downstream of the confocal diaphragm unit, a detector, and an imaging unit which images the partial beams on the detector in a spatially separated manner. The confocal diaphragm unit is switchable between a confocal diaphragm with exactly m apertures for m-spot illumination and a confocal diaphragm with n apertures for n-spot illumination. The splitting unit has a first beam path for m-spot illumination and a second beam path for n-spot illumination. The splitting unit is switchable between the two beam paths.Type: GrantFiled: February 28, 2013Date of Patent: June 5, 2018Assignee: Carl Zeiss Microscopy GmbHInventors: Tiemo Anhut, Daniel Schwedt, Ralf Wolleschensky, Lars-Christian Wittig, Ulrich Preiβer
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EVALUATION OF SIGNALS OF FLUORESCENCE SCANNING MICROSCOPY USING A CONFOCAL LASER SCANNING MICROSCOPE
Publication number: 20180113292Abstract: A method for evaluating signals of fluorescence scanning microscopy with simultaneous excitation and detection of fluorescence in different focal planes of a sample by means of confocal laser scanning microscopy.Type: ApplicationFiled: May 9, 2016Publication date: April 26, 2018Inventors: Yauheni NOVIKAU, Thomas KALKBRENNER, Tiemo ANHUT, Daniel SCHWEDT, Matthias WALD -
Patent number: 9857578Abstract: A laser scanning microscope for the acquisition of object images according to varied observation criteria. The microscope includes an illumination and detection unit, an illumination and a detection beam, a microscope objective, a scanning device with a scanning optical component and several scanners, with switching mirrors, each mirror with two switching positions, provided in the illumination and detection beams. Each switching position is assigned to one of several different, optically separate beam paths and each beam path defines a separate operating mode. A concave mirror for imaging a first scanner into at least one more scanner and vice versa is arranged in at least one of the beam paths.Type: GrantFiled: November 5, 2015Date of Patent: January 2, 2018Assignees: Carl Zeiss Microscopy GmbH, Carl Zeiss AGInventors: Beate Boehme, Matthias Wald, Tiemo Anhut, Daniel Schwedt