Abstract: The invention relates to a process for purifying and concentrating charge-bearing first molecules 9, such as proteins, nucleic acids and the like, comprising the following steps: a) preparation of a solution containing the first molecules 9, b) contacting the solution with an electrode 2 which is directly provided with a coating of second molecules 4 having affinity for the first molecules 9, and c) connecting the electrode 2 to a means 11 for generating an electric field to bring about a movement of the first molecules 9 in the solution directed relative to the electrode 2.

Abstract: The invention relates to an agent for detecting chemical substances, comprising: a first polynucleotide or peptide sequence having a first fluorophore molecule, the first end of said sequence being bonded on a solid phase and a second polynucleotide or peptide sequence having a second fluorophore molecule, the second end of said sequence having a group that is sensitive to the chemical substance to be detected. The first polynucleotide or peptide sequence can be hybridized to the second polynucleotide or peptide sequence in such a way that a spatial relationship enabling an interaction betweeen the first and the second fluorophore molecule is produced. When the chemical substance including a physical property is attached to the group and the influence of an external force is exerted on the physical property, the spatial relation can be eliminated, thereby generating or abolishing a fluorescent reaction.

Abstract: A method for preparing samples for detecting a nucleotide sequence by polymerase chain reaction (PCR) according to which a) an analysis solution is filled into at least one cavity (2) provided for in a support (1); b) a lid (3) configured complementary to the shape of the cavity (2) is placed onto the support (1) in such a way that the analysis solution is pushed at least partly into a gap (S) formed between the cavity (2) and the lid (3); and c) the gap (S) is sealed by means of at least one seal (5, 12) provided for near an opening in the cavity (2).

Abstract: A method is disclosed for detecting a nucleotide sequence by a polymerase chain reaction, the nucleotide sequence is a constituent of a double-stranded nucleic acid molecule which is in solution and formed of a strand and a counter-strand. A first primer is added to the solution and is complementary to a first segment of the strand located at the 5′-terminus of a central segment. A second primer is added and is complementary to a 5′-terminal second segment of the counter-strand. The solution is brought into contact with a third primer whose 5′-terminal end is bound to a solid phase. The third primer is complementary to the central segment of the strand containing the nucleotide sequence. A reaction vessel is closed which contains the solution. The solution is repeatedly heated and cooled and the nucleic acid molecule is detected in the closed reaction vessel.

Abstract: The invention relates to a method for preparing samples for detecting a nucleotide sequence by polymerase chain reaction (PCR), according to which a) an analysis solution is filled into at least one cavity (2) provided for in a support (1); b) a lid (3) configured complementary to the shape of the cavity (2) is placed onto the support (1) in such a way that the analysis solution is pushed at least partly into a gap (S) formed between the cavity (2) and the lid (3); and c) the gap (S) is sealed by means of at least one seal (5, 12) provided for near an opening in the cavity (2).

Abstract: The invention refers to a cap for containers for holding a mixture to be produced from two components, having a cylinder (5) with an open end and an end closed by a partition (6) which can be perforated, a piston (1) having a gripping piece (2) of larger design than the cylinder diameter being inserted into the open end of the cylinder, such that a first chamber (K1) having a height (h) is formed between a lower side (KU) of the piston lying opposite the gripping piece (2) and the partition (6), characterized in that two incisions (7) extending from the open end of the cylinder and lying opposite each other are provided in the cylinder wall, and in that the gripping piece (2) is designed in such a manner that it can be displaced in the incisions (7) in order to move the piston (1) in the direction of the partition (6).

Abstract: The invention relates to a method of detecting and identifying nucleic aicd (NA) molecules within a population (I) of NA molecules of identical, similar or different sequence, in which: (a) the single-stranded or duplex ribonucleic acid (RNA) molecules or single-stranded deoxyribonucleic aicd (DNA) molecules contained in a population (I) iare convereted into a population (II) of duplex DNA molecules; b) the population (II) of duplex DNA molecules is subjected to denaturation and subsequent renaturation, so that a mixture of homo- and heteroduplex DNA molecules is present; c) heteroduplexes formed in the population (II) where unpaired of incorrectly paired nucleotides are present are reacted chemically with a carbodiimide compound; and d) the resulting carboddimide reactions products are characterized and quantified, characterized in that a chromatographic purification method is carried out between each of the steps a to d.

Abstract: A method for preparing samples for detecting a nucleotide sequence by polymerase chain reaction (PCR) according to which a) an analysis solution is filled into at least one cavity (2) provided for in a support (1); b) a lid (3) configured complementary to the shape of the cavity (2) is placed onto the support (1) in such a way that the analysis solution is pushed at least partly into a gap (S) formed between the cavity (2) and the lid (3); and c) the gap (S) is sealed by means of at least one seal (5, 12) provided for near an opening in the cavity (2).

Abstract: The invention relates to a device for holding a pipette in a pipetting aid, permitting gastight insertion of the pipette into the pipetting aid without contact pressure having to be exerted by the person handling it, spreader elements (2) being cast in a soft rubber or plastic aperture bead of the pipetting aid, and it being possible for the spreader elements (2) to have their positions relative to each other changed by tensioning elements (3) in such a way that the aperture bead can be widened for the insertion of the pipette.

Abstract: The invention relates to a method for marking solid, liquid or gaseous substances, whereby the substance to be marked is provided with at least one synthetically produced nucleic acid sequence. Said nucleic acid sequence contains a first sequence section constructed with the 5′ terminal end, a second sequence comprised of at least two bases and connected to said nucleic acid sequence, and a third sequence section constructed with the 3′ terminal end and connected to the said nucleic acid sequence. In order to simplify the identification of the marking, the invention provides that a first primer group is used with a first primer section corresponding to the first sequence section and a second primer group is used with a third primer section corresponding to the third sequence section.

Abstract: The invention relates to a recombinant poliovirus vector and method of making the vector. The recombinant poliovirus vector comprises an RNA polymerase promoter operably linked to a poliovirus nucleic acid sequence which has been altered by removal of the CelII-SnaBI fragment and insertion of a heterologous nucleic acid sequence encoding a protein of interest. The heterologous nucleic acid sequence is flanked on both sides by internal ribosomal entry sites which allow for the expression of the encoded protein of interest and subsequent proteins encoded by the altered poliovirus nucleic acid sequence.

Abstract: The invention relates to a vectorial cloning system consisting of a sequence of nucleotides, containing a fusion sequence and one or several restriction endonucleases, recognition sites for restriction endonucleases cutting outside their recognition sites, in addition to containing one or several other restriction endonuclease recognition sites which can be used to clone a foreign protein as well as the sequence for the desired foreign protein, wherein the foreign protein sequence is directly located on the fusion sequence after a subsequent restriction with the restriction endonucleases, followed by religation.

Abstract: The invention relates to a vehicle for the transport of molecular substance, such as DNA, RNA, protein, PNA, pharmaceuticals of lipophilic and lipophobic character, into eukaryotic cells comprising at least one capsomere derived or originating from a virus, which on one of its sides interacts with the molecular substance such that the molecular substance can be bound or added to the capsomere.

Abstract: Method and device for processing nucleic acids in a reaction mixture on a surface that can be temperature-controlled and its immediate vicinity wherein the main space of the reaction mixture remains essentially isothermal. The method has the advantage of a very short processing time.

Abstract: A method for altering a cell by exchanging a preselected cellular DNA sequence with an exogenous DNA sequence different from the cellular DNA sequence employs an exogenous DNA sequence encapsidated in a polyoma or polyoma-like capsid. The polyoma capsid is then contacted to the cell so that the exogenous DNA sequence is introduced within the cell and exchanges with the preselected cellular DNA sequence by homologous recombination.A preferred article of manufacture comprises a polyoma capsid and a plurality of DNA sequences encapsulated within the polyoma capsid. The DNA sequences each comprise not more than an incomplete portion of a single preselected gene.The exogenous DNA sequence may optionally be complexed to a DNA binding protein, such as a recA protein, prior to encapsulating the exogenous DNA sequence within a viral capsid, so that the uptake of the DNA sequence into the capsid is enhanced.