Abstract: Provided are a vesicular adaptor and a single-chain cyclic library constructed by using the adaptor. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantage of high throughput sequencing, high accuracy and simple operations.

Abstract: Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.

Abstract: The current invent relates to the construction and application of a three-line breeding system in the bay scallop×Peruvian scallop hybrids. The said three-line consists of a male sterile line, a maintainer line and a restorer line. A combination of male sterile line and maintainer line is obtained by continuously backcrossing the male sterile individuals selected from F1 inter-specific hybrid families with sperm of the selfing family of the sperm-providing Peruvian scallops until the progenies are all male sterile. The restorer line is obtained by continuously backcrossing selected individuals from the male sterile line with sperm of the selfing family of a Peruvian scallop until the progenies are all hermaphroditic and exhibit excellent production traits. Commercial male sterile brood stocks are produced by backcrossing the male sterile line with the maintainer line and commercial hybrid spats are produced by backcrossing the male sterile line and the restorer line.

Abstract: The current invent relates to the construction and application of a three-line breeding system in the bay scallop southern subspecies x Peruvian scallop hybrids. The said three-line consists of a male sterile line, a maintainer line and a restorer line. A combination of male sterile line and maintainer line is obtained by continuously backcrossing the male sterile individuals selected from F1 inter-specific hybrid families with sperm of the selfing family of the sperm-providing Peruvian scallops until the progenies are all male sterile. The restorer line is obtained by continuously backcrossing selected individuals from the male sterile line with sperm of the selfing family of a Peruvian scallop until the progenies are all hermaphroditic and exhibit excellent production traits. Commercial male sterile brood stocks are produced by backcrossing the male sterile line with the maintainer line and commercial hybrid spats are produced by backcrossing the male sterile line and the restorer line.

Abstract: Provided is an apparatus for preparing a building structure with 3D printing, comprising: a 3D printing device (1), having a storage chamber (11) and a printing head (12) connected to the storage chamber (11) and being movable relative to a base frame; and a reinforcing device, movably disposed relative to the base frame, and having a driving mechanism (3) for driving short rebars or short ribs to perform an insertion movement in a direction intersected with a stacking direction of a cement-based slurry layer; the driving mechanism inserts the short rebars or the short ribs into an interlayer interface spanning at least two adjacent cement-based slurry layers printed by the 3D printing device (1). Also provided a method for preparing a building structure with 3D printing.

Abstract: The present invention belongs to the field of biological medicine, particularly to a novel tumor vaccine. In order to solve the problem in the art that no technical scheme is available for generating lasting and high-effective anti-tumor immune responses, the present invention provides a tumor vaccine mainly containing a complex as a main active ingredient, wherein the complex is formed by nucleic acid, especially replicable nucleic acid not expressing exogenous gene, and cationic biomaterial. The nucleic acid and the cationic biomaterial in the tumor vaccine according to the present invention have synergistic interactions on direct killing of tumor cells, and induction of the innate immune response and adaptive immune response of body against tumor. In addition, the prepared tumor vaccine has simple drug component and is easy to produce and maintain quality control. The tumor vaccine has a good prospect for application.

Abstract: Provided are a vesicular adaptor and a single-chain cyclic library constructed by using the adaptor. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantage of high throughput sequencing, high accuracy and simple operations.

Abstract: Methods, computer program products, and systems are presented. The methods include, for instance: synchronizing, by one or more processor, first virtual machine(s) with a second virtual machine, the synchronizing comprising mirroring first input events to the first virtual machine(s); and customizing, by the one or more processor, the first virtual machine(s), the customizing comprising suspending the synchronizing and facilitating sending of second input events to the first virtual machine(s). In one embodiment, the synchronizing comprises cloning the second virtual machine to create the first virtual machine(s), the cloning comprising initializing the first virtual machine(s), and copying second virtual machine state information to the first virtual machine(s).

Abstract: Provided are a vesicular linker and a single-chain cyclic library constructed by using the linker. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantages of high throughput sequencing, high accuracy and simple operations.

Abstract: A pharmaceutical syringe piston having a longitudinal axis includes a body having a central face portion with a first outer circumference and maximum first diameter. A cylindrical sealing portion proximate the central face portion has first and second axial ends and a wall extending therebetween. The wall has a second outer circumference and a second diameter essentially constant along the longitudinal axis between the first and second axial ends of the cylindrical sealing portion and is larger than the maximum first diameter. An annular curved portion has a maximum outer diameter less than the second diameter and connects the first axial end of the cylindrical sealing portion to the first outer circumference. An inert film encloses the central face portion and at least part of the annular curved portion and has an outer boundary between the first axial end of the cylindrical sealing portion and the first outer circumference.

Abstract: Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5? end of the long strand has a phosphoric acid modification, and the 3? end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3? end thereof can be in a complementary pairing with the 5? end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.

Abstract: A method and reagent for constructing a nucleic acid double-linker single-strand cyclic library.

Abstract: Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.

Abstract: Provided is a method of constructing a sequencing library using an adaptor element in a bubble shape. The bubble-shaped adaptor is ligated to a DNA fragment respectively at the 3?-terminal and the 5?-terminal i.e., two bubble-shaped adaptors with same sequences are ligated in one step. The bubble-shaped adaptor-ligated product is then amplified with a primer complementary to the 3?-terminal of the long-chain nucleic acid of the bubble-shaped adaptor, so as to replace the non-paired sequence in the short-chain nucleic acid.

Abstract: A tamper-evident closure for containers includes a closure shell having a top surface and a depending skirt. The top surface defines a first aperture therethrough and a frangible portion. The skirt defines an open lower end for engaging a container. A tamper-evident tearable member overlies the top surface of the closure shell and defines a second aperture overlying the first aperture. A removable closure cap overlies the top surface of the closure shell and the tearable member. The closure cap has an annular ring projecting downwardly from an underside of the closure cap and extending through the first and second apertures. The annular ring attaches to an underside of the frangible portion. At least partial removal of the closure cap from the top surface of the closure shell peels the frangible portion away from the top surface of the closure shell along the scoring, and tears the tamper-evident member.

Abstract: A pharmaceutical syringe piston having a longitudinal axis includes a body having a central face portion with a first outer circumference and maximum first diameter. A cylindrical sealing portion proximate the central face portion has first and second axial ends and a wall extending therebetween. The wall has a second outer circumference and a second diameter essentially constant along the longitudinal axis between the first and second axial ends of the cylindrical sealing portion and is larger than the maximum first diameter. An annular curved portion has a maximum outer diameter less than the second diameter and connects the first axial end of the cylindrical sealing portion to the first outer circumference. An inert film encloses the central face portion and at least a part of the annular curved portion and has an outer boundary between the first axial end of the cylindrical sealing portion and the first outer circumference.

Abstract: A method and device for determining static equivalences of distribution networks is provided. The method comprises: acquiring node admittance matrices related to a border node and a distribution network, power loss and a voltage value and a current value of the border node; constructing static equivalence determination conditions for determining static equivalences of the distribution network, comprising a consistency of sensitivity equation, a consistency of power loss equation, an equivalent network power balance equation and a Kirchhoff's current equation; and calculating static equivalent parameters according to the static equivalence determination conditions. The inventive method and device are simple and effective, where the consistency of sensitivity and power loss before and after equivalence are considered comprehensively, and where the distributed generators' voltage and power support characteristics in an active distribution network are reserved.

Abstract: The present disclosure discloses a method and device for determining static equivalences of distribution networks, relating to the technical field of static equivalences of power systems. The method comprises: acquiring node admittance matrices related to a border node and a distribution network, power loss and a voltage value and a current value of the border node; constructing static equivalence determination conditions for determining static equivalences of the distribution network, comprising a consistency of sensitivity equation, a consistency of power loss equation, an equivalent network power balance equation and a Kirchhoff's current equation; and calculating static equivalent parameters according to the static equivalence determination conditions.

Abstract: Provided are a method for constructing a nucleic acid single-stranded cyclic library and the reagents used therein. By the combination of interruption via a transposase with a restricted nick translation reaction, the method realizes a simple and rapid nucleic acid single-stranded cyclic library construction.

Abstract: Methods, computer program products, and systems are presented. The methods include, for instance: synchronizing, by one or more processor, first virtual machine(s) with a second virtual machine, the synchronizing comprising mirroring first input events to the first virtual machine(s); and customizing, by the one or more processor, the first virtual machine(s), the customizing comprising suspending the synchronizing and facilitating sending of second input events to the first virtual machine(s). In one embodiment, the synchronizing comprises cloning the second virtual machine to create the first virtual machine(s), the cloning comprising initializing the first virtual machine(s), and copying second virtual machine state information to the first virtual machine(s).