Abstract: The present invention provides a novel method for ligating an adapter to a target polynucleotide and methods of generating a library of mate-pair polynucleotide constructs that employ such a ligation method. Libraries and arrays comprising mate-pair polynucleotide constructs, and methods of sequencing libraries and arrays comprising mate-pair polynucleotide constructs, are also provided.

Abstract: Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions at two terminals and a non-paired region in the middle.

Abstract: Methods, computer program products, and systems are presented. The methods include, for instance: synchronizing, by one or more processor, first virtual machine(s) with a second virtual machine, the synchronizing comprising mirroring first input events to the first virtual machine(s); and customizing, by the one or more processor, the first virtual machine(s), the customizing comprising suspending the synchronizing and facilitating sending of second input events to the first virtual machine(s). In one embodiment, the synchronizing comprises cloning the second virtual machine to create the first virtual machine(s), the cloning comprising initializing the first virtual machine(s), and copying second virtual machine state information to the first virtual machine(s).

Abstract: Provided are a vesicular linker and a single-chain cyclic library constructed by using the linker. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantages of high throughput sequencing, high accuracy and simple operations.

Abstract: A method and reagent for constructing a nucleic acid double-linker single-strand cyclic library.

Abstract: A method and reagent for constructing a nucleic acid double-joint single-strand cyclical library.

Abstract: A modulation policy for a modular multi-level convertor, determining a switching state of each submodule by combining the current direction of each bridge arm and the capacitor voltage order of the submodules based on a carrier stacking method. The beneficial effects of the modulation policy are that: each phase only needs a modulation wave and N carriers, given N submodules of each upper bridge arm or lower bridge arm of the modular multi-level convertor; the modular multi-level convertor can output N+1 levels without carrier phase shift; N devoted submodules of each phase are guaranteed at any time; and the submodules' capacitor voltage balancing can be controlled without a closed-loop control policy. The modulation policy facilitates adjustment of the modular multi-level convertor voltage and power class, has unlimited number of levels, has a high precision control algorithm, is easy to realize in engineering and saves software and hardware resources.

Abstract: The present invention discloses a manager, a test agent installed on a personal mobile device and methods thereof. The manager comprises: a first network connection module configured to establish a connection with the mobile device through Internet, the mobile device being installed with a test agent for performing test operation on a mobile application on the mobile device; and a security module configured to communicate with the test agent through the first network connection module to make the test agent perform security control on the mobile device. According to the manager, mobile devices, and methods of the present invention, the cost such as maintenance cost of the data center and purchase cost of mobile devices can be reduced dramatically. It is not necessary to analyze market demands since mobile devices owned by the users of the mobile devices are the mobile devices that need to be tested by the tester.

Abstract: The current invent relates to the construction and application of a three-line breeding system in the bay scallop southern subspecies×Peruvian scallop hybrids. The said three-line consists of a male sterile line, a maintainer line and a restorer line. A combination of male sterile line and maintainer line is obtained by continuously backcrossing the male sterile individuals selected from F1 inter-specific hybrid families with sperm of the selfing family of the sperm-providing Peruvian scallops until the progenies are all male sterile. The restorer line is obtained by continuously backcrossing selected individuals from the male sterile line with sperm of the selfing family of a Peruvian scallop until the progenies are all hermaphroditic and exhibit excellent production traits. Commercial male sterile brood stocks are produced by backcrossing the male sterile line with the maintainer line and commercial hybrid spats are produced by backcrossing the male sterile line and the restorer line.

Abstract: The current invent relates to the construction and application of a three-line breeding system in the bay scallop×Peruvian scallop hybrids. The said three-line consists of a male sterile line, a maintainer line and a restorer line. A combination of male sterile line and maintainer line is obtained by continuously backcrossing the male sterile individuals selected from F1 inter-specific hybrid families with sperm of the selfing family of the sperm-providing Peruvian scallops until the progenies are all male sterile. The restorer line is obtained by continuously backcrossing selected individuals from the male sterile line with sperm of the selfing family of a Peruvian scallop until the progenies are all hermaphroditic and exhibit excellent production traits. Commercial male sterile brood stocks are produced by backcrossing the male sterile line with the maintainer line and commercial hybrid spats are produced by backcrossing the male sterile line and the restorer line.

Abstract: The present invention provides a mining method. The method includes: dividing a mining region into a plurality of federated mining regions; performing an open-pit mining operation in each of the federated mining regions and forming a pit in each of the federated mining regions; performing an underground mining operation on a slope of the pit and forming a plurality of excavated tunnels; and backfilling a pit of a previous federated mining region with a spoil of a subsequent federated mining region.

Abstract: Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5? end of the long strand has a phosphoric acid modification, and the 3? end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3? end thereof can be in a complementary pairing with the 5? end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.

Abstract: A tamper-evident closure for containers includes a closure shell having a top surface and a depending skirt. The top surface defines a first aperture therethrough and a frangible portion. The skirt defines an open lower end for engaging a container. A tamper-evident tearable member overlies the top surface of the closure shell and defines a second aperture overlying the first aperture. A removable closure cap overlies the top surface of the closure shell and the tearable member. The closure cap has an annular ring projecting downwardly from an underside of the closure cap and extending through the first and second apertures. The annular ring attaches to an underside of the frangible portion. At least partial removal of the closure cap from the top surface of the closure shell peels the frangible portion away from the top surface of the closure shell along the scoring, and tears the tamper-evident member.

Abstract: A tamper-evident closure (110) for containers includes a closure shell (112) having a top surface (118) and a depending skirt (120). The top surface defines a first aperture (124) therethrough and a frangible portion (122). The skirt defines an open lower end (20a) for engaging a container. A tamper-evident tearable member (114) overlies the top surface of the closure shell and defines a second aperture (126) overlying the first aperture. A removable closure cap (116) overlies the top surface of the closure shell and the tearable member. The closure cap has an annular ring (132) projecting downwardly from an under-side of the closure cap and extending through the first and second apertures. The annular ring attaches to an underside of the frangible portion. At least partial removal of the closure cap from the top surface of the closure shell peels the frangible portion away from the top surface of the closure shell along the scoring, and tears the tamper-evident member.

Abstract: Methods, computer program products, and systems are presented. The methods include, for instance: synchronizing, by one or more processor, first virtual machine(s) with a second virtual machine, the synchronizing comprising mirroring first input events to the first virtual machine(s); and customizing, by the one or more processor, the first virtual machine(s), the customizing comprising suspending the synchronizing and facilitating sending of second input events to the first virtual machine(s). In one embodiment, the synchronizing comprises cloning the second virtual machine to create the first virtual machine(s), the cloning comprising initializing the first virtual machine(s), and copying second virtual machine state information to the first virtual machine(s).

Abstract: The present invention provides a mining method. The method includes: dividing a mining region into a plurality of federated mining regions; performing an open-pit mining operation in each of the federated mining regions and forming a pit in each of the federated mining regions; performing an underground mining operation on a slope of the pit and forming a plurality of excavated tunnels; and backfilling a pit of a previous federated mining region with a spoil of a subsequent federated mining region.

Abstract: A tamper-evident closure (110) for containers includes a closure shell (112) having a top surface (118) and a depending skirt (120). The top surface defines a first aperture (124) therethrough and a frangible portion (122). The skirt defines an open lower end (20a) for engaging a container. A tamper-evident tearable member (114) overlies the top surface of the closure shell and defines a second aperture (126) overlying the first aperture. A removable closure cap (116) overlies the top surface of the closure shell and the tearable member. The closure cap has an annular ring (132) projecting downwardly from an under-side of the closure cap and extending through the first and second apertures. The annular ring attaches to an underside of the frangible portion. At least partial removal of the closure cap from the top surface of the closure shell peels the frangible portion away from the top surface of the closure shell along the scoring, and tears the tamper-evident member.

Abstract: The present invention relates to emulsion-templated silica micro and nano-capsules- and methods for making them. In particular, the template emulsion is stabilized by a biosurfactant that also assists in nucleating the silica shell Mineralizing biosurfactants and stabilized micro- and nano-emulsions useful in forming the emulsion-templated micro- and nano-capsules, and methods for the use of the silica micro- and nano-capsules are also described.

Abstract: A pharmaceutical syringe piston having a longitudinal axis includes a body having a central face portion with a first outer circumference and maximum first diameter. A cylindrical sealing portion proximate the central face portion has first and second axial ends and a wall extending therebetween. The wall has a second outer circumference and a second diameter essentially constant along the longitudinal axis between the first and second axial ends of the cylindrical sealing portion and is larger than the maximum first diameter. An annular curved portion has a maximum outer diameter less than the second diameter and connects the first axial end of the cylindrical sealing portion to the first outer circumference. An inert film encloses the central face portion and at least a part of the annular curved portion and has an outer boundary between the first axial end of the cylindrical sealing portion and the first outer circumference.

Abstract: The invention describes image sensor array pixels with global and rolling shutter capabilities that utilize multiple BCMD transistors for storing and sensing charge for a single photodiode. This configuration improves the Dynamic Range (DR) of the sensor, by allowing sensing different image signals from a single pixel without saturation, a low level signal with long integration time followed by a high level signal with short integration time. Signal processing circuits can process these signals into a single Wide Dynamic Range (WDR) output. Further disclosed are pixels that use multiple-gate BCMD transistors for charge storage and sensing having multiple concentric gates, which allows changing the conversion gain of the BCMD transistors. Variable conversion gain is a useful feature when building WDR sensors since low conversion gain and high well capacity allows detection of high level signals and, at the same time, low level signals with high conversion gain and low noise.