Patents by Inventor Xiaolian Gao

Xiaolian Gao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 8053187
    Abstract: A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.
    Type: Grant
    Filed: October 4, 2010
    Date of Patent: November 8, 2011
    Assignee: The Regents of the University of Michigan
    Inventors: Xiaolian Gao, Hua Zhang, Peilin Yu, Eric Leproust, Jean Philippe Pellois, Qin Xiang, Xiaochuan Zhou
  • Publication number: 20110184163
    Abstract: Nucleotide triphosphate probes containing a molecular and/or atomic tag on a a ? and/or ? phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.
    Type: Application
    Filed: January 6, 2011
    Publication date: July 28, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Susan H. Hardin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu
  • Publication number: 20110170075
    Abstract: This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. Specifically, the method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.
    Type: Application
    Filed: November 19, 2010
    Publication date: July 14, 2011
    Inventors: Xiaolian Gao, Xiaochuan Zhou, Erdogan Gulari
  • Publication number: 20110143964
    Abstract: The present invention describes microfluidic devices that provide novel fluidic structures to facilitate the separation of fluids into isolated, pico-liter sized compartments for performing multiplexing chemical and biological reactions. Applications of the novel devices including biomolecule synthesis, polynucleotide amplification, and binding assays are also disclosed.
    Type: Application
    Filed: February 18, 2005
    Publication date: June 16, 2011
    Inventors: Xiachuan Zhou, Erdogan Gulari, Xiaolian Gao
  • Publication number: 20110097762
    Abstract: A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.
    Type: Application
    Filed: October 4, 2010
    Publication date: April 28, 2011
    Applicant: THE REGENTS OF THE UNVERSITY OF MICHIGAN
    Inventors: Xiaolian Gao, Hua Zhang, Peilin Yu, Eric Leproust, Jean Philippe Pellois, Qin Xiang, Xiaochuan Zhou
  • Patent number: 7923550
    Abstract: The present invention describes novel compounds and methods for capping reactive groups on support and during multistep synthesis. These new capping reagents are also useful for high quality synthesis on solid supports and surfaces used as microarrays, biosensors, or in general as biochips. The compounds are also useful for controlling surface density of reactive groups on a support. The compounds may also be used to modify the hydrophilic/hydrophobic characteristics of a surface or a molecule. The compounds have functional utility in various applications in the fields of genomics, proteomics, diagnostics and medicine.
    Type: Grant
    Filed: April 14, 2004
    Date of Patent: April 12, 2011
    Inventors: Xiaolian Gao, Peilin Yu
  • Publication number: 20110059436
    Abstract: A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
    Type: Application
    Filed: April 7, 2009
    Publication date: March 10, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Susan Hardin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu
  • Publication number: 20110021383
    Abstract: A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
    Type: Application
    Filed: March 26, 2009
    Publication date: January 27, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Susan Hardin, James Briggs, Shiao-Chun Tu, Xiaolian Gao, Richard Willson
  • Publication number: 20110014604
    Abstract: A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
    Type: Application
    Filed: March 24, 2009
    Publication date: January 20, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: SUSAN HARDIN, XIAOLIAN GAO, JAMES BRIGGS, RICHARD WILLSON, SHIAO-CHUN TU
  • Publication number: 20110008775
    Abstract: The present invention relates to the field of analysis of nucleic acid sequences. More specifically, the present invention relates to the method and instrument for high throughput parallel DNA sequencing. The present invention also provides method for selection of sequences from analyte samples for enrichment of the target sequences or depletion of the selected molecules and in particular undesirable sequence templates from sequencing samples.
    Type: Application
    Filed: December 10, 2008
    Publication date: January 13, 2011
    Inventors: Xiaolian Gao, Xiaochuan Zhou
  • Publication number: 20100304367
    Abstract: A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
    Type: Application
    Filed: March 26, 2009
    Publication date: December 2, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Susan Hardin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu
  • Patent number: 7838466
    Abstract: This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. In particular, the present invention provides methods and devices for selectively converting photogenerated reagent precursors to photogenerated reagents comprising a substrate comprising at least one solid surface containing a plurality of isolated reaction sites; and an optical system operably linked to the substrate comprising a light source and a computer-controlled spatial optical modulator to form an irradiation pattern, wherein the irradiation pattern selectively irradiates a plurality of reaction sites without use of a photolithographic mask. The method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.
    Type: Grant
    Filed: May 29, 2002
    Date of Patent: November 23, 2010
    Assignees: The Regents of the University of Michigan, University of Houston
    Inventors: Xiaolian Gao, Xiaochuan Zhou, Erdogan Gulari
  • Publication number: 20100286370
    Abstract: The present invention relates to novel fluorescent proteins and to methods of making these proteins and the uses thereof.
    Type: Application
    Filed: December 4, 2009
    Publication date: November 11, 2010
    Inventor: Xiaolian Gao
  • Publication number: 20100255464
    Abstract: Nucleotide triphosphate probes containing a molecular and/or atomic tag on a ? and/or ? phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.
    Type: Application
    Filed: December 29, 2006
    Publication date: October 7, 2010
    Inventors: Susan H. Hardin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu
  • Publication number: 20100255463
    Abstract: A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
    Type: Application
    Filed: December 29, 2006
    Publication date: October 7, 2010
    Inventors: Susan Harsin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu
  • Patent number: 7807807
    Abstract: A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.
    Type: Grant
    Filed: June 29, 2009
    Date of Patent: October 5, 2010
    Assignee: The Regents of the University of Michigan
    Inventors: Xiaolian Gao, Hua Zhang, Peilin Yu, Eric Leproust, Jean Philippe Pellois, Qin Xiang, Xiaochuan Zhou
  • Publication number: 20100216122
    Abstract: Nucleotide triphosphate probes containing a molecular and/or atomic tag on a ? and/or ? phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.
    Type: Application
    Filed: January 12, 2009
    Publication date: August 26, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Susan H. Hardin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu
  • Publication number: 20100210478
    Abstract: The present invention relates to quantitative and quantity aspects of array synthesis and array uses as a device for high capacity producing synthetic molecules for off-array surface applications and as an assay device for on-array surface applications.
    Type: Application
    Filed: June 29, 2007
    Publication date: August 19, 2010
    Inventors: Xiaolian Gao, Kiaochun Zhou, Xiaolin Zhang, Ailing Hong, Qi Zhu, Peilin Yu
  • Publication number: 20100137600
    Abstract: This invention describes reagent precursors and methods for chemical and biochemical reactions. These reagent precursors that can be activated in solution upon irradiation to generate reagents required for the subsequent chemical reactions. Specifically, photogenerated reagents (PGR) are useful for controlling parallel combinatorial synthesis and various chemical and biochemical reactions.
    Type: Application
    Filed: May 18, 2009
    Publication date: June 3, 2010
    Inventors: Xiaolian Gao, Zhuo Xiaochruan, Wu Yao, Jean-Phillipe Pollols
  • Publication number: 20100112558
    Abstract: The present invention relates to the field of methods and devices of miniaturized synthesis. More specifically, the present invention relates to the parallel synthesis of large number of different types of molecules and oligomers, such as oligonucleotides (oligos), peptides, lipids, carbohydrates, small ligand molecules, and other organic and inorganic molecules as probes for multiplexing assays. The probes may be synthesized from and/or attached to nanobeads to microbeads. The present invention provides for assays of multiplexing large scale biology, such as analysis of genomic DNAs and RNAs and proteomic proteins or peptides performed simultaneously on the synthetic beads.
    Type: Application
    Filed: November 3, 2008
    Publication date: May 6, 2010
    Inventors: Xiaolian Gao, Xiaochuan Zhou