Patents by Inventor Xiaolian Gao

Xiaolian Gao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20100093974
    Abstract: A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.
    Type: Application
    Filed: June 29, 2009
    Publication date: April 15, 2010
    Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Xiaolian Gao, Hua Zhang, Peilin Yu, Eric Leproust, Jean Philippe Pellois, Qin Xiang, Xiaochuan Zhou
  • Publication number: 20090305278
    Abstract: A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
    Type: Application
    Filed: April 6, 2009
    Publication date: December 10, 2009
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Susan Hardin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu
  • Publication number: 20090275036
    Abstract: A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
    Type: Application
    Filed: March 30, 2009
    Publication date: November 5, 2009
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Susan Hardin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu
  • Patent number: 7553958
    Abstract: A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.
    Type: Grant
    Filed: March 21, 2007
    Date of Patent: June 30, 2009
    Assignee: The Regents of the University of Michigan
    Inventors: Xiaolian Gao, Hua Zhang, Peilin Yu, Eric Leproust, Jean Philippe Pellois, Qin Xiang, Xiaochuan Zhou
  • Patent number: 7544638
    Abstract: This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. In particular, the present invention provides methods and devices for generating a photogenerated reaction in solution on a substrate comprising generating a light beam using an optical device system comprising a light source and a computer-controlled spatial optical modulator comprising a digital micromirror device and using the spatial optical modulator to direct light to fluidly isolated reaction sites under conditions such that a photogenerated reaction takes place. The method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.
    Type: Grant
    Filed: January 13, 2003
    Date of Patent: June 9, 2009
    Assignees: The Regents of the University of Michigan, University of Houston
    Inventors: Xiaolian Gao, Xiaochuan Zhou, Erdogan Gulari
  • Patent number: 7544829
    Abstract: This invention describes reagent precursors and methods for chemical and biochemical reactions. These reagent precursors that can be activated in solution upon irradiation to generate reagents required for the subsequent chemical reactions. Specifically, photogenerated reagents (PGR) are useful for controlling parallel combinatorial synthesis and various chemical and biochemical reactions.
    Type: Grant
    Filed: December 29, 2006
    Date of Patent: June 9, 2009
    Inventors: Xiaolian Gao, Zhou Xiaochuan, Wu Yao, Jean-Phillipe Pellois
  • Patent number: 7491680
    Abstract: This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. In some embodiments, the present invention provides methods and devices for generating one or more selected multimers at specific reaction sites on a substrate comprising contacting the substrate with a liquid solution comprising photo-reagent precursors, isolating the reaction sites, selectively irradiating the reaction sites with a digital micromirror device, and contacting the substrate with monomers. The method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.
    Type: Grant
    Filed: September 11, 2002
    Date of Patent: February 17, 2009
    Assignees: The Regents of the University of Michigan, University of Houston
    Inventors: Xiaolian Gao, Xiaochuan Zhou, Erdogan Gulari
  • Publication number: 20080318806
    Abstract: This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. In particular, the present invention provides methods and devices for generating a photogenerated reaction in solution on a substrate comprising generating a light beam using an optical device system comprising a light source and a computer-controlled spatial optical modulator comprising a digital micromirror device and using the spatial optical modulator to direct light to fluidly isolated reaction sites under conditions such that a photogenerated reaction takes place. The method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.
    Type: Application
    Filed: January 13, 2003
    Publication date: December 25, 2008
    Applicant: The Regents Of The University Of Michigan
    Inventors: Xiaolian Gao, Xiaochuan Zhou, Erdogan Gulari
  • Publication number: 20080248971
    Abstract: An interactive method of providing an array of nucleic acid sequences in which a remote user enters a query to generate a listing of desired sequence probes, which are then selected and returned to the host for use in producing a custom microarray designed by a remote user.
    Type: Application
    Filed: March 10, 2008
    Publication date: October 9, 2008
    Applicant: INVITROGEN CORPORATION
    Inventors: Xiaolian Gao, Xiaoming Wang, Jerry Liu
  • Publication number: 20080091005
    Abstract: Modified nucleotides are disclosed for use in single molecule sequencing, methods for making the modified nucleotides and method for using the modified nucleotides. Linkers for making the modified nucleotide are also disclosed.
    Type: Application
    Filed: July 20, 2007
    Publication date: April 17, 2008
    Applicants: VISIGEN BIOTECHNOLOGIES, INC., APPLIED BIOSYSTEMS
    Inventors: Hongyi Wang, Xiaolian Gao, Peilin Yu, Mitsu Reddy, Susan Hardin, Tommie Lincecum, Amy Williams, Norha Deluge, Yuri Belosludtsev, Steven Menchen, Joe Lam, Jer-Kang Chen
  • Patent number: 7329492
    Abstract: A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
    Type: Grant
    Filed: December 8, 2004
    Date of Patent: February 12, 2008
    Assignee: Visigen Biotechnologies, Inc.
    Inventors: Susan Hardin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu
  • Publication number: 20080009630
    Abstract: This invention describes reagent precursors and methods for chemical and biochemical reactions. These reagent precursors that can be activated in solution upon irradiation to generate reagents required for the subsequent chemical reactions. Specifically, photogenerated reagents (PGR) are useful for controlling parallel combinatorial synthesis and various chemical and biochemical reactions.
    Type: Application
    Filed: December 29, 2006
    Publication date: January 10, 2008
    Inventors: Xiaolian Gao, Zhou Xiaochuan, Wu Yao, Jean Pellois
  • Publication number: 20070292867
    Abstract: A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
    Type: Application
    Filed: December 29, 2006
    Publication date: December 20, 2007
    Inventors: Susan Hardin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu
  • Publication number: 20070287832
    Abstract: A method of modulation of synthesis capacity on and cleavage properties of synthetic oligomers from solid support is described. The method utilizes linker molecules attached to a solid surface and co-coupling agents that have similar reactivities to the coupling compounds with the surface functional groups. The preferred linker molecules provide an increased density of polymers and more resistance to cleavage from the support surface. The method is particularly useful for synthesis of oligonucleotides, oligonucleotides microarrays, peptides, and peptide microarrays. The stable linkers are also coupled to anchor molecules for synthesis of DNA oligonucleotides using on support purification, eliminating time-consuming chromatography and metal cation presence. Oligonucleotides thus obtained can be directly used for mass analysis, DNA amplification and ligation, hybridization, and many other applications.
    Type: Application
    Filed: March 21, 2007
    Publication date: December 13, 2007
    Inventors: Xiaolian Gao, Hua Zhang, Peilin Yu, Eric Leproust, Jean Pellois, Qin Xiang, Xiaochuan Zhou
  • Publication number: 20070275395
    Abstract: A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
    Type: Application
    Filed: December 29, 2006
    Publication date: November 29, 2007
    Inventors: Susan Hardin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu
  • Publication number: 20070224616
    Abstract: A method for forming molecular sequences includes derivatizing an unconfined substrate surface with at least one linker containing a protected reactive group. The substrate is contacted with a solution containing a photogenerated reagent precursor and a buffer and/or a neutralizer. A photogenerated reagent is generated in at least a portion of the solution. The photogenerated reagent is configured to initiate the formation of at least one active region on the substrate surface. A monomer is coupled to the active region.
    Type: Application
    Filed: March 23, 2007
    Publication date: September 27, 2007
    Inventors: Erdogan Gulari, Jean-Marie Rouillard, Xiaolian Gao, Xiaochuan Zhou
  • Publication number: 20070184475
    Abstract: A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
    Type: Application
    Filed: December 29, 2006
    Publication date: August 9, 2007
    Inventors: Susan Hardin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu
  • Publication number: 20070172869
    Abstract: Nucleotide triphosphate probes containing a molecular and/or atomic tag on a ? and/or ? phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.
    Type: Application
    Filed: December 29, 2006
    Publication date: July 26, 2007
    Inventors: Susan Hardin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu
  • Publication number: 20070172863
    Abstract: A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
    Type: Application
    Filed: December 29, 2006
    Publication date: July 26, 2007
    Inventors: Susan Hardin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu
  • Publication number: 20070172859
    Abstract: Nucleotide triphosphate probes containing a molecular and/or atomic tag on a a ? and/or ? phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.
    Type: Application
    Filed: December 29, 2006
    Publication date: July 26, 2007
    Inventors: Susan Hardin, Xiaolian Gao, James Briggs, Richard Willson, Shiao-Chun Tu