Compositions of iodophenoxy alkylene ethers in film-forming materials for visualization of the gastrointestinal tract

- Sterling Winthrop Inc.

R=C.sub.1 -C.sub.25 alkyl, cycloalkyl, ##STR2## or halo-lower-alkyl, each of which may be optionally substituted with halo, fluoro-lower-alkyl, aryl, lower-alkoxy, hydroxy, carboxy, lower-alkoxy carbonyl or lower-alkoxy-carbonyloxy, (CR.sub.1 R.sub.2).sub.p --(CR.sub.3 =CR.sub.4).sub.m Q ,or (CR.sub.1 R.sub.2).sub.p --C.tbd.C--Q;R.sub.1, R.sub.2 and R.sub.3 and R.sub.4 are independently H, or lower-alkyl, optionally substituted with halo;x is 1-4;n is 1-4;m is 1-15;p is 1-20; andQ is H, lower-alkyl, lower-alkenyl, lower-alkynyl, lower-alkylene, aryl, or aryl-lower alkylThese compounds are combined in a polymer for use as an X-ray contrast agent.

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Description
BACKGROUND OF THE INVENTION

1. Field of the Invention

This invention relates to x-ray contrast compositions containing the contrast agent iodophenoxy alkylene ethers, and methods for their use in diagnostic radiology of the gastrointestinal tract.

2. Reported Developments

Roentgenographic examination utilizing X-rays and computed tomography (hereinafter CT) scans of fractures and other conditions associated with the skeletal system is routinely practiced without the use of contrast agents. X-ray visualization of organs containing soft tissue, such as the gastrointestinal (hereinafter GI) tract, requires the use of contrast agents which attenuate X-ray radiation. D. P. Swanson et al in "Pharmaceuticals In Medical Imaging", 1990, MacMillan Publishing Company, provides an excellent background in medical imaging utilizing contrast agents and compositions therewith.

Roentgenographic examination of the GI tract are indicated for conditions of digestive disorders, changes in bowel habit, abdominal pain, GI bleeding and the like. Prior to radiological examination, administration of a radiopaque contrast medium is necessary to permit adequate delineation of the respective lumen or mucosal surface from surrounding soft tissues. Accordingly, a contrast medium is administered orally to visualize the mouth, pharynx, esophagus, stomach, duodenum and proximal small intestine. The contrast medium is administered rectally for examination of the distal small intestine and the colon.

The most widely used contrast agent for the visualization of the GI tract is barium sulfate administered as a suspension orally or rectally as an enema. (See, for example, U.S. Pat. Nos. 2,659,690; 2,680,089; 3,216,900; 3,235,462; 4,038,379 and 4,120,946) Notwithstanding its relatively good contrast characteristics, negligible absorption from the GI tract following oral or rectal administration and speedy excretion from the body, barium sulfate has certain disadvantages. In the presence of intestinal fluids it lacks homogeneity and poorly adheres to mucus membranes which can result in poor X-ray images. In the colon, when administered as an enema, it flocculates and forms irregular clumps with fecal matter.

Iodinated organic compounds have also been used as GI contrast agents since the iodine atom is an effective X-ray absorber. They have the most versatility and are utilized in the widest variety of procedures. They are very absorptive of X-rays with which the iodine interacts and produce a so-called photoelectric effect which is a large magnification in contrast caused by the photons stopped in the iodine-containing medium. The magnification of contrast exceeds the level that would be expected from relative changes in density. Because of this magnification, relatively low concentrations of the contrast agent can be utilized. (For iodinated agents see, for example, U.S. Pat. Nos. 2,786,055; 3,795,698; 2,820,814; 3,360,436; 3,574,718, 3,733,397; 4,735,795 and 5,047,228.)

The desiderata for an ideal GI contrast agent includes: good toxicological profile; the ability to fill the entire bowel/lumen and evenly coat the gut mucosa so that the presence of the bowel is detectable when the lumen is not distended; and nonirritation to the intestinal mucosa; and passage through the GI tract without producing artifacts or stimulating vigorous intestinal peristalsis.

These requirements were addressed by many investigators and their efforts resulted in great improvements over the years. The requirement of evenly coating the gut mucosa with a contrast agent to effectively cover the walls of the intestines proved to be rather difficult. Without meeting these requirements it is impossible to obtain X-ray pictures of high precision. To that end, the use of certain polymer additives were proposed as illustrated hereunder.

U.S. Pat. No. 4,069,306 discloses an X-ray contrast preparation which is said to adhere to the walls of body eavities. The preparation comprises a finely divided water-insoluble inorganic X-ray contrast agent and minute particles of a hydrophilic polymer which is insoluble in water but is water-swellable. The body cavity is supplied with such preparation suspended in water. The X-ray contrast agent is present in admixture with and/or enclosed in and/or adhered to said minute polymer particles.

U.S. Pat. No. 4,120,946 discloses a pharmaceutical composition for barium opacification of the digestive tract, comprising colloidal barium sulfate and a polyacrylamide in an aqueous vehicle. The polyacrylamide forms a viscous solution at low concentration which makes it possible to maintain the barium sulfate in suspension and at the same time permit good adherence of the preparation to the walls of the organ which it is desired to X-ray.

U.S. Pat. No. 5,019,370 discloses a biodegradable radiographic contrast medium comprising biodegradable polymeric spheres which carry a radiographically opaque element, such as iodine, bromine, samarium and erbium. The contrast medium is provided either in a dry or liquid state and may be administered intravenously, orally and intra-arterially.

While these polymeric materials greatly enhance attachment of the contrast agent used therewith to the walls of organs for better visualization thereof, they do not provide a uniform coating thereon. As such, there is still a need for an improved X-ray imaging medium that uniformly coats the soft tissues subjected to diagnostic X-ray examination.

SUMMARY OF THE INVENTION

It is the object of the present invention to provide compositions for coating the gastrointestinal tract of mammals to form an effective radiopaque coating thereon by which diagnostic examination of the GI tract may be accomplished. To that end, a thin coating is formed on the inner surface of the GI tract effected by ingesting, prior to visualization by an X-ray emitting device, a polymeric film former, which has incorporated therein an X-ray contrast agent, capable of coating the GI tract. The removal of the coating occurs as a result of the nominal turnover of cells, that is, within about 24 to 48 hours. Such compositions must meet several requirements: both the X-ray contrast agent and the film former must be nontoxic; must not contain leachable or digestible components that would deleteriously affect the patient; and the composition must be capable of forming a film in the pH range of from about 5 to about 8.

The object of the present invention is achieved by a composition comprising: an X-ray contrast agent; a polymeric material which is at least partially water soluble and contains polarizable or ionizable groups; and a divalent metal ion selected from the group consisting of Mg.sup.++, Ca.sup.++, Zn.sup.++ and Bal.sup.++ which potentiates the effect of the polymeric material as a film former on the mucosa of the GI tract.

The contrast agent, polymeric film former and the divalent metal ion are incorporated in a solid or liquid media for administration to a mammal for X-ray visualization of the GI tract.

In accordance with the invention there is further provided a method for x-ray diagnostic imaging of the GI tract which comprises orally or rectally administering to the patient an effective contrast producing amount of one of the above-described x-ray contrast compostions.

The composition for radiological examination of the GI tract comprises a compound of the formula: ##STR3## ps wherein Z=H, halo, C.sub.1 -C.sub.20 alkyl, cycloalkyl, lower alkoxy, alkoxycarbonyl, cyano, where the alkyl and cycloalkyl groups can be substituted with halogen or halo-lower-alkyl groups;

R=C.sub.1 -C.sub.25 alkyl, cycloalkyl, ##STR4## or halo-lower-alkyl, each of which may be optionally substituted with halo, fluoro-lower-alkyl, aryl, lower-alkoxy, hydroxy, carboxyl, lower-alkoxy carbonyl or lower-alkoxy-carbonyloxy, (CR.sub.1 R.sub.2).sub.p --(CR.sub.3 =CR.sub.4).sub.m Q, or (CR.sub.1 R.sub.2).sub. --C.tbd.C--Q;

R.sub.1, R.sub.2, R.sub.3 and R.sub.4 are independently H, or lower-alkyl, optionally substituted with halo;

x is 1-4;

n is 1-4;

m is 1-15;

p is 1-20; and

Q is H, lower-alkyl, lower-alkenyl, lower-alkynyl, lower-alkylene, aryl, or aryl-lower alkyl.

As used herein, the term halogen (or halo) means fluorine, chlorine, bromine or iodine.

As used herein, the term cycloalkyl means carbocyclic rings having from three to eight ring carbon atoms including cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and cyclooctyl which may be substituted on any ring carbon atom thereof by one of more lower-alkyl groups, lower-alkoxy groups or halogens.

As used herein the terms lower-alkyl and lower-alkoxy mean monovalent aliphatic radicals, including branched chain radials, of from one to ten carbon atoms. Thus, the lower-alkyl moiety of such groups include, for example, methyl, ethyl, propyl, isopropyl, n-butyl, sec-butyl, t-butyl, n-pentyl, 2-methyl-3-butyl, 1-methylbutyl, 2-methylbutyl, neopentyl, n-hexyl, 1-methylpentyl, 3-methylpentyl, 1-ethylbutyl, 2-ethylbutyl, 2-hexyl, 3-hexyl, 1,1,3,3-tetramethylpentyl, 1,1-dimethyloctyl and the like.

As used herein, the term lower-alkenyl and lower-alkynyl means monovalent, unsaturated radicals including branched chain radicals of from three to ten carbon atoms and thus include 1-ethenyl, 1-(2-propenyl), 1-(2-butenyl), 1-(1-methyl-2-propenyl), 1-(4-methyl-2-pentenyl), 4,4,6-trimethyl-2-heptenyl, 1-ethynyl, 1-(2-propynyl), 1-(2-butynyl), 1-(1-methyl-2-propynyl), 1-(4-methyl-2-pentynyl) and the like.

As used herein, the term alkylene means divalent saturated radicals, including branched chain radicals of from two to ten carbon atoms having their free valences on different carbon atoms and thus includes 1,2-ethylene, 1,3-propylene, 1,4-butylene, 1-methyl-1, 2-ethylene, 1,8-octylene and the like.

As used herein, the term aryl means an aromatic hydrocarbon radical having six to ten carbon atoms. The preferred aryl groups are phenyl, substituted phenyl and naphthyl substituted by from one to three, the same or different members of the group consisting of lower-alkyl, halogen, hydroxy-lower-alkyl, alkoxy-lower-alkyl and hydroxy.

The x-ray contrast compound can comprise one, two, three or more iodine atoms per molecule; preferred species contain at least two, and more preferably, at least three iodine atoms per molecule.

The solid x-ray contrast agents in particulate forms useful in the practice of the present invention can be prepared by techniques known in the art. The solid agents are comminuted to the desired size using conventional milling methods, such as airjet or fragmentation milling. We have found that an effective average particle size of less than about 100.mu. provides for good distribution and coating in the GI tract. As used herein, particle size refers to a number average particle size as measured by conventional techniques, such as sedimentation field flow fractionation and disk centrifugation. An effective average particle size of less than about 100.mu. means that at least about 90% of the particles have a weight average particle size of less than about 100.mu. as measured by art recognized techniques.

The polymers that were found to be suitable for forming a thin coating on the GI tract can be classified as anionic, cationic and neutral polymers, a description of which follows. U.S. Pat. No. 4,623,539, the disclosure of which is incorporated by reference, pertains to such polymers.

The contrast agent is incorporated in the polymeric material along with the divalent cation by any suitable techniques, such as by mixing, blending, precipitating or by enclosing the contrast agent into minute polymeric particles.

The contrast agent, polymeric material and divalent cation blend is then formulated for administration using physiologically acceptable carriers or excipients in a manner within the skill of the art. The contrast agent with the addition of pharmaceutically acceptable aids (such as surfactants and emulsifiers) and excipients may be suspended or partially dissolved in an aqueous medium resulting in a dispersion, solution, suspension or emulsion. Alternatively, the contrast agent, polymeric material and divalent cation may be formulated into a solid form, such as tablets or capsules.

A method for diagnostic imaging of the GI tract for use in medical procedures in accordance with this invention comprises orally or rectally administering to the mammalian patient in need of x-ray examination, an effective contrast producing amount of a composition of the present invention. After administration, at least a portion of the GI tract containing the administered composition is exposed to x-rays to produce an x-ray image pattern corresponding to the presence of the contrast agent, then the x-ray image is visualized and interpreted using techniques known in the art.

DETAILED DESCRIPTION OF THE INVENTION

Compounds of the present invention can be made according to the schematic procedure shown or other methods using commercially available starting materials, intermediates and reagents. Starting materials, reagents and solvents can be obtained from chemical supplies such as Aldrich, Baker and Eastman Chemical Companies, or they may be prepared by techniques known in the art.

The following examples will further illustrate the compounds used in the present invention.

EXAMPLE 1 Bis-(4-iodophenyl) ether of polyethylene-glycol-400 ##STR6##

A. Preparation of bis-mesylate of polyethylene-glycol-400 (PEG-400)

To a solution of PEG-400 (40.0 g, 100 mmol) in dry CH.sub.2 CH.sub.2 (200 ml) at 4.degree. C was added diisopropylethylamine (43.5 ml, 250 mmol). After 10 min., methanesulfonyl chloride (17.0 ml, 220 mmol) was added as a solution in CH.sub.2 Cl.sub.2 (40 ml) over a period of 0.4 hr. After 2.5 hrs, the reaction was diluted to 500 ml with cold CH.sub.2 Cl.sub.2 and poured over ice-cold 1 N aqueous HCl (400 ml). The layers were separated. The organic layer was washed with water (300 ml) and brine (300 ml), dried (Na.sub.2 SO.sub.4), filtered and evaporated in vacuo to provide the bis-mesylate as a light yellow oil (56.0 g, 99%), .sup.1 H NMR spectrum was satisfactory.

B. Preparation of Bis-(4-iodophenyl) ether of polyethylene glycol-400

The bis-mesylate of PEG-400 (15.3 g, 27.5 mmol) in dry DMF (110 ml) was reacted with 4-iodophenol (12.1 g, 55.0 mmol) and potassium carbonate (7.6 g, 55.0 mmol) at 77.degree. C. under an atmosphere of N.sub.2. After stirring for 16 hrs, the reaction was allowed to cool, diluted with DMF, filtered through a pad of celite and evaporated in vacuo. The resulting residue was taken up with EtOAc (500 ml), washed with 1 M aqueous sodium hydroxide (200 ml), water (200 ml) and brine (200 ml), dried (Na.sub.2 SO.sub.4), filtered, and evaporated in vacuo to provide a light brown syrup. Flash column chromatography (silica, 1%-5%; CH.sub.3 OH in CH.sub.2 Cl.sub.2) provided bis-(4-iodophenyl) ether of polyethylene glycol-400 which was slightly contaminated with unreacted mesylates. The product was further purified by dissolving 10.5 g in DMF (100 ml). Sodium methoxide (400 mg) was added. The reaction flask was immersed in an oil bath and warmed to 93.degree. C. After stirring for 16 hrs, the reaction was allowed to cool, diluted with EtOAc (500 ml), washed with water (200 ml) and brine (200 ml) repeatedly (3 times), dried (Na.sub.2 SO.sub.4), filtered and evaporated in vacuo to provide a light yellow syrup. Flash column chromatography (silica, 5% CH.sub.3 OH in CH.sub.2 Cl.sub.2) provided bis-(4-iodophenyl) ether of polyethylene glycol-400 as a light yellow syrup (8.3 g). .sup.1 H NMR (300 MHz) analysis of the product indicates that the average number of ethylene oxide units is eight (n=8).

Title Compound: .sup.1 H (300 MHz) and .sup.13 C (75 MHz) NMR spectra were consistent with the desired product.

EXAMPLE 2 1,8-Bis-O-(2,4,6-triiodophenyl)-tripropylene glycol ##STR7##

A. Preparation of tripropylene glycol-di-p-tosylate

To a solution of tripropylene glycol (19.3 g, 100 mmol) in anhydrous toluene (400 ml) was added p-toluenesulfonyl chloride (76.6 g, 401 mmol), triethylamine (62.7 ml, 450 mmol) and dimethylamino pyridine (1.22 g, 10 mmol). The reaction was immersed in an oil bath which was warmed to 66.degree. C. over 0.5 hr. After stirring under N.sub.2 for 20 hrs, the reaction was allowed to cool, diluted with toluene (500 ml), filtered through a pad of celite and evaporated in vacuo. The resulting residue was dissolved in CH.sub.2 Cl.sub.2 (1 liter), washed with 5% aqueous sodium bicarbonate (500 ml), water (500 ml) and brine (500 ml), dried (Na.sub.2 SO.sub.4), filtered and evaporated in vacuo. Flash column chromatography (silica, CH.sub.2 Cl.sub.2) afforded tripropylene glycol di-p-tosylate (9.6 g, 19.2%) as a viscous yellow oil.

B. Preparation of 1,8-bis-O-(2.4.6-triiodophenyl)-triprolpylene glycol

To a stirred solution of tripropylene glycol di-p-tosylate (9.5 g, 18.9 mmol) in dry DMF (76 ml) was added triiodophenol (18.8 g, 39.8 mmol) and potassium carbonate (5.48 g, 39.7 mmol). The reaction was immersed in an oil bath which was heated to 76.degree. C. The reaction was stirred at 76.degree. C. for 41 hrs. At the end of this period the reaction was diluted with DMF (200 ml), filtered through a pad of celite and evaporated in vacuo. The resulting residue was dissolved in ether (300 ml), washed with 1 M aqueous sodium hydroxide (100 ml), water (2.times.100 ml) and brine, dried (Na.sub.2 SO.sub.4), filtered and evaporated in vacuo. Flash column chromatography (silica, 1:1, CH.sub.2 Cl.sub.2 : hexanes) provided 1,8-bis-O-(2,4,6-triiodophenyl)-tripropylene glycol (14.8 g, 13.4 mmol) as a light yellow glass.

Title Compound: .sup.1 H (300 MHz) and .sup.13 C (75 MHz) NMR spectra were consistent with the desired product. Calculated for C.sub.21 H.sub.22 O.sub.4 I.sub.6 : C, 22.93; H, 2.02; 1, 69.23. Found: C, 23.23, H, 2.00; 1, 69.55.

EXAMPLE3 1.1 1-bis-(2.4.6-triiodophenoxy)-3.6.9-trioxaundecane ##STR8##

To a solution of tetraethylene glycol di-p-tosylate (15.0 g, 29.8 mmol) in DMF (120 ml) was added triiodophenol (31.0 g, 65.7 mmol) and potassium carbonate (9.0 g, 65.2 mmol). The reaction flask was immersed in an oil bath which was heated to 74.degree. C. over a period of 0.5 hr. The reaction was stirred under an N.sub.2 atmosphere for 17 hrs. At the end of this period, TLC analysis indicated the reaction was complete. The reaction was cooled, diluted with DMF and filtered through a pad of celite. The filtrate was evaporated in vacuo to yield a light brown solid which was stirred with ethyl acetate and filtered to yield a light grey solid (21.0 g). A portion of this product (10 g) was recrystallized from hexanes to give 1,11-bis-(2,4,6-triiodophenoxy)-3,6,9-trioxaundecane (9.4 g) as a white solid. Mp (from hexanes): 126.degree.-128.degree. C.

Title Compound: .sup.1 H (300 MHz) and .sup.13 C (75 MHz) NMR spectra were consistent with the desired product. FAB/MS: MH.sup.+ : 1103. Calculated for C.sub.20 H.sub.20 O.sub.5 I.sub.6, C 21.80; H, 1.83; 1, 69.11. Found: C, 22.18, H, 1.79; 1, 69.41.

EXAMPLE 4 1,2-Bis-(2,4,6-Triiodophenoxy)-ethane ##STR9##

Using the procedure described for the preparation of 1,12-bis-(2,4,6-triiodophenoxy)-dodecane, 1,2-bis-(2,4,6-triiodophenoxy)ethane was prepared in 32% yield from triiodophenol (1.87 g, 3.96 mmol), milled potassium carbonate (0.66 g, 4.78 mmol, 1.2 eq) and dibromoethane (0.18 MI, 1.98 mmol) in DMF (12 ml) at 60.degree. C. for 12 hours. Recrystallization from DMF gave white needles (1.2 g, 32%), Mp. 288.degree.-289.degree. C. after drying under high vacuum/110.degree. C. Title Compound: .sup.1 H (300 MHz) and .sup.13 C (75 MHz) NMR spectra were consistent with the desired product. Calculated for C.sub.14 H.sub.8 I.sub.6 O.sub.2 : C, 17.34; H, 0.83; 1, 78.53; Found: C, 17.60, H, 0.82; 1, 78.30.

EXAMPLE 5 Bis-O-(2,4,6-triiodophenyl) ether of polyethylene glycol 400 ##STR10##

The bis-mesylate of polyethylene glycol-400 (18.7 g, 33.6 mmol) was reacted with triiodophenol (36.5 g, 77.4 mmol) and potassium carbonate (10.7 g, 77.5 mmol) in dry DMF (13.6 ml) as per bis-O-(2,4,6-triiodophenyl) ether of polyethylene glycol 400 except at a temperature of 76.degree. C. for 14 hrs followed by heating at 92.degree. C. for 4 hrs. At the end of this period, the reaction was allowed to cool, diluted with DMF, filtered through celite and evaporated in vacuo. The residue was taken up in EtOAc (600 ml), washed with water (250 ml), 1 N aqueous sodium hydroxide (250 ml), water (2.times.250 ml) and brine (250 ml), dried (Na.sub.2 SO.sub.4), filtered and evaporated in vacuo to give a dark brown syrup. Flash column chromatography (silica, 1:39-1:19, CH.sub.3 OH:CH.sub.2 Cl.sub.2) provided a brown syrup. This product was further purified by dissolution in EtOAc (500 ml), washing with saturated aqueous sodium thiosulfate (2.times.200 ml) and brine (200 ml), drying (Na.sub.2 SO.sub.4) and filtering. The filtrate was treated with decolorizing carbon (10 g), heated to boiling (0.25 h), filtered and evaporated in vacuo to provide bis-O-(2,4,6-triiodophenyl) ether of polyethylene glycol-400 (29 g). .sup.1 H NMR analysis (300 MHZ) of the product indicates that the average number of the ethylene oxide units is eight (n=8). Title Compound: .sup.1 H (300 MHz) and .sup.13 C (75 MHz) NMR spectra were consistent with the desired product.

EXAMPLE 6 1-(3-Iodophenoxy)-3.6.9-trioxadecane ##STR11##

A. Preparation of the mesylate of triethylene glycol monomethyl ether

The mesylate was prepared via standard protocol from triethylene glycol monomethyl ether (8.2 g, 50.0 mmol), methanesulfonyl chloride (4.6 ml, 59.4 mmol) and diisopropylethylamine (11.3 ml, 64.9 mmol) in dichloromethane (100 ml) in 99% yield.

B. Preparation of 1-(3-Iodophenoxy)-3,6,9-trioxadecane

To a solution of 1-[(methanesulfonyl)-oxyl-3,6,9-trioxadecane (11.6 g, 47.9 mmol) in dry DMF (145 ml) was added 3-iodophenol (10.6 g, 48.2 mmol) and potassium carbonate (6.6 g, 47.8 mmol). The reaction was immersed in an oil bath which was warmed to 68.degree. C. over a period of 0.5 hr. The reaction was stirred at this temperature under an N.sub.2 atmosphere for 16 hrs and then at 82.degree. C. for an additional 2 hrs. At the end of this period, the reaction was cooled, diluted with DMF, filtered through a pad of celite and evaporated in vacuo. The resulting residue was taken up into ethyl acetate (500 ml), washed with water (200 ml), 1 N aqueous sodium hydroxide (200 ml) and brine (200 ml), dried (Na.sub.2 SO.sub.4), filtered and evaporated in vacuo to provide a light brown oil. Flash column chromatography (silica, 1:3 to 1:2; EtOAc:hexane) provided 1 -(3-iodophenoxy)-3,6,9-trioxadecane as a light yellow oil (10.8 g, 37.1%).

Title Compound: .sup.1 H (300 MHz) and .sup.13 C (75 MHz) NMR spectra were consistent with the desired product. Calculated for C.sub.13 H.sub.10 O.sub.4 I: C, 42.52; H, 5.49; 1, 34.56. Found: C, 42.50, H, 5.13; 1, 34.78.

EXAMPLE 7 1,3-Bis-(2.4,6-Triiodolophenoxy)-butane ##STR12##

A mixture of triiodophenol (5.78 g, 12.3 mmol) and sodium hydride (0.49 g, 60% dispersion, 12.3 mmol) in DMF (15 ml) was stirred at room temperature for 1 hour and then a solution of 1,3-bis-(methanesulfonyloxy) butane (1.37 g, 5.58 mmol) in DMF (5 ml) was added. The mixture was heated to 90.degree. C. for 6 hours and then poured into water after cooling. Ethyl acetate was added and the mixture was allowed to stand overnight. The precipitated solid was collected and dried to give 3.0 g (54%) of the desired product, Mp 173.degree.-175.degree. C.

Title Compound: .sup.1 H (300 MHz) and .sup.13 C (75 MHz) NMR spectra were consistent with the desired product. Calculated for C.sub.16 H.sub.12 I.sub.6 O.sub.2 : C, 18.26; H, 1.21; 1, 76.32; Found: C,19.35, H, 1.16; 1, 76.27.

EXAMPLE 8 1(3-Iodophenoxy)-6(2,4,6-triiodophenoxy)-hexane ##STR13##

A mixture of potassium carbonate (3.0 g, 21.9 mmol, 1.1 eq), 3-iodophenol (4.4 g, 19.9 mmol) and 6-(2,4,6-triiodophenoxy)-1-chlorohexane (11.75 g, 19.9 mmol) in 50 ml of DMF was heated to 110.degree. C. for 1.5 hours and then cooled. The mixture was poured into water and extracted with ethyl acetate. The organic layer was dried (magnesium sulfate) and evaporated to give a solid which was filtered through basic alumina (hexanes-ethyl acetate). The filtrate was concentrated under vacuum leaving the crude product as a solid. Recrystallization from cyclohexane afforded 8.29 g (54%) of the desired product after drying at room temperature under high vacuum, mp 65.degree.-66.degree. C.

Title Compound: .sup.1 H (300 MHz) and .sup.13 C (75 MHz) NMR spectra were consistent with the desired product. Calculated for C.sub.18 H.sub.18 I.sub.4 O.sub.2 : C, 27.93; H, 2.34; I, 65.59; Found; C, 27.85, H, 2.16; I, 65.53.

EXAMPLE 9 1,12-Bis(2,4,6-Triiodophenoxy)-dodecane ##STR14##

A mixture of triiodophenol (14.4 g, 30.5 mmol), potassium carbonate (4.6 g, 33.6 mmol, 1.1 eq) and 1,12-dibromododecane (5.0 g, 15.2 mmol) in dimethylformamide (30 ml) was heated at 120.degree. C. for 2 hours and then cooled. The reaction mixture was poured into water (400 ml) and ethyl acetate (400 ml) was added. The solid that precipitated was collected, slurried in boiling methanol and then boiling water. The residual solid was recrystallized from dimethylformamide to give the product as a white solid (11.1 g, 66%), mp. 120.degree.-121.degree. C. after drying under high vacuum (0.2 torr) at 90.degree. C.

Title Compound: .sup.1 H (300 MHz) and .sup.13 C (75 MHz) NMR spectra were consistent with the desired product. Calculated for C.sub.24 H.sub.28 I.sub.6 O.sub.2 : 1/4 ((CH.sub.3).sub.2 NCHO): C, 26.35; H, 2.66; I, 67.49; Found: C,26.64, H, 2.47; 1, 67.38.

Compositions of the Present Invention

The contrast agents may be formulated for administration using physiologically acceptable carriers or excipients in a manner within the skill of the art. The compounds with the addition of pharmaceutically acceptable aids (such as surfactants and emulsifiers) and excipients may be suspended or partially dissolved in an aqueous medium resulting in a dispersion, solution or suspension. However, the oily contrast agents are preferably made into emulsions.

Compositions of the present invention comprise the following pharmaceutically acceptable components based on % w/v:

  ______________________________________                                    
     Non-aqueous phase      1-50                                               
     Polymeric Material  0.001-15                                              
     Divalent Cation     0.001-15                                              
     Contrast Agent      0.001-75                                              
     Excipient              0-20                                               
     Aids/Surfactants/Emulsifiers)                                             
                          0.01-15                                              
     Water               q.s. to 100                                           
     ______________________________________

Specific Examples of the compositions of the present invention are shown in Examples 12-14.

EXAMPLE NO. 12

Bis-(4-iodophenyl) ether of polyethylene-glycol-400: 23.7 % (W/V)

Safflower Oil: 20.0 % (w/v)

Kappa Carrageenan: 2.0 % (w/v)

Calcium Lactate: 2.0 % (w/v)

Tween: 21 2.5 % (w/v)

Hydroxypropylmethylcellulose (4000 cPs): 0.5 % (w/v)

q.s. with water to 100% volume and shake

EXAMPLE NO. 13

1,8-Bis-O-(2,4,6-triiodophenyl)-tripropylene glycol: 55.3 % (w/v)

Dow Corning Medical Antifoam AF: 40.0 % (w/v)

Pectin: 4.0 % (w/v)

Calcium Lactate: 2.0 % (w/v)

q.s. with water to 100% volume and shake

EXAMPLE NO. 14

1,11-bis-(2,4,6-triiodophenoxy)-3,6,9-trioxaundecane: 25.9 % (W/V)

Simplesse.RTM. Dietary Fat Substitute: 30.0 % (w/v)

Heparin: 1.0 % (W/V)

Magnesium Carbonate: 1.0 %(W/V)

Hydroxypropylmethylcellulose (4000 cPs): 0.5 % (w/v)

q.s. with water to 100% volume and shake

The nonaqueous phase comprises vegetable oils such as safflower oil; non-metabolizing fat substituents, such as Simplesse; fluorinated hydrocarbons, such as perfluorodecalin; mineral oil and simethicone.

Excipients advantageously used in the formulations include viscosity mediating and stabilizing agents, such as microcrystalline cellulose, ethylcellulose, hydroxypropyl methylcellulose and gum arabic. Physiologically acceptable substances may also be included, such as sodium citrate, sodium chloride, therapeutic substances, antacid substances and flavoring agents. The inclusion of antimicrobial/antiseptic agents such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, benzoic acid or sorble acid may also be desirable in some formulations.

As known by those skilled in the art, surfactants or emulsifiers can reduce the interfacial tension between two immiscible phases, i.e., oil-in-aqueous medium. These agents can be used alone or in combination with other emulsifying agents and surfactants. For example, Dow Corning Medical Antifoam AF, which is a composition of 30% w/v polydimethylsiloxane simethicone and silica aerogel, 14% w/v stearate emulsifiers and 0.075% w/v sorbic acid, the balance being water, may be used by itself. Intralipid, which is an emulsion of fatty acids needs the presence of a suspending agent for it to form an acceptable emulsion with contrast agents of the present invention. The amount of such surfactants may be in the range of from 0.01 to 15% w/v of the aqueous formulations, although the amount, in general, is kept as low as possible, preferably in the range of 0.05 to 5% w/v. The surface active agents may be cationic, anionic, nonionic, zwitteiionic or a mixture of two or more of these agents.

Suitable cationic surfactants include cetyl trimethyl ammonium bromide. Suitable anionic agents include sodium lauryl sulphate, sodium heptadecyl sulphate, alkyl benzenesulphonic acids and salts thereof, sodium butylnapthalene sulfonate, and sulphosuccinates. Zwitterionic surface active agents are substances that when dissolved in water they behave as diprotic acids and, as they ionize, they behave both as a weak base and a weak acid. Since the two charges on the molecule balance each other out the molecules act as neutral molecules. The pH at which the zwitterion concentration is maximum is known as the isoelectric point. Compounds, such as certain amino acids having an isoelectric point at the desired pH of the formulations of the present invention are useful in practicing the present invention.

In preparing the formulations of the present invention we prefer to use nonionic emulsifiers or surface active agents which, similarly to the nonionic contrast agents, possess a superior toxicological profile to that of anionic, cationic or zwitterionic agents. In the nonionic emulsifying agents the proportions of hydrophilic and hydrophobic groups are about evenly balanced. They differ from anionic and cationic surfactants by the absence of charge on the molecule and, for that reason, are generally less of an irritant than the cationic or anionic surfactants. Nonionic surfactants include carboxylic esters, carboxylic amides, ethoxylated alkylphenols and ethoxylated aliphatic alcohols.

One particular type of carboxylic ester nonionic surface active agents are the partial, for example mono-, esters formed by the reaction of fatty and resin acids, for example of about 8 to about 18 carbon atoms, with polyhydric alcohols, for example glycerol, glycols such as mono-, di-, tetra- and hexaethylene glycol, sorbitan, and the like; and similar compounds formed by the direct addition of varying molar ratios of ethylene oxide to the hydroxy group of fatty acids.

Another type of carboxylic esters is the condensation products of fatty and resin partial acids, for example mono-, esters ethylene oxide, such as fatty or resin acid esters of polyoxyethylene sorbitan and sorbitol, for example polyoxyethylene sorbitan, monotall oil esters. These may contain, for example, from about 3 to about 80 oxyethylene units per molecule and fatty or resin acid groups of from about 8 to about 18 carbon atoms. Examples of naturally occurring fatty acid mixtures which may be used are those from coconut oil and tallow while examples of single fatty acids are dodecanoic acid and oleic acid.

Carboxylic amide nonionic surface active agents are the ammonia, monoethylamins and diethylamine amides of fatty acids having an acyl chain of from about 8 to about 18 carbon atoms.

The ethoxylated alkylphenol nonionic surface active agents include various polyethylene oxide condensates of alkylphenols, especially the condensation products of monoalkylphenols or dialkylphenols wherein the alkyl group contains about 6 to about 12 carbon atoms in either branched chain or particularly straight chain configuration, for example, octyl cresol, octyl phenol or nonyl phenol, with ethylene oxide, said ethylene oxide being present in amounts equal to from about 5 to about 25 moles of ethylene oxide per mole of alkylphenol. Ethoxylated aliphatic alcohol nonionic surface active agents include the condensation products of aliphatic alcohols having from about 8 to 18 carbon atoms in either straight chain or branched chain configuration, for example oleyl or cetyl alcohol, with ethylene oxide, said ethylene oxide being present in equal amounts from about 30 to about 60 moles of ethylene oxide per mole of alcohol.

Preferred nonionic surface active agents include: sorbitan esters (sold under the trade name Span) having the formula: ##STR15## wherein R.sub.1 =R.sub.2 =OH, R.sub.3 =R for sorbitan monoesters,

R.sub.1 =OH, R.sub.2 =R.sub.3 =R for sorbitan diesters,

R.sub.1 =R.sub.2 =R.sub.3 =R for sorbitan triesters,

where

  ______________________________________                                    
            R =  (C.sub.11 H.sub.23) COO for laurate,                          
                 (C.sub.17 H.sub.33) COO for oleate,                           
                 (C.sub.15 H.sub.31) COO for palmitate,                        
                 (C.sub.17 H.sub.35) COO for stearate.                         
     ______________________________________

Polyoxyethylene alkyl ethers (i.e. Brijs) having the formula:

CH.sub.3 (CH.sub.2).sub.x (O-CH.sub.2 -CH.sub.2)YOH

where (x+1) is the number of carbon atoms in the alkyl chain, typically:

  ______________________________________                                    
     12 lauryl            (dodecyl)                                            
     14 myristyl          (tetradecyl)                                         
     16 cetyl             (hexadecyl)                                          
     18 stearyl           (octadecyl)                                          
     ______________________________________

and y is the number of ethylene oxide groups in the hydrophilic chain, typically 10-60.

Polyethylene sorbitan fatty acid esters, sold under the trade names of Polysorbates 20, 40, 60, 65, 80 and 85.

Polyethylene stearates, such as:

poly(oxy-1,2-ethanediyl),.alpha.-hydro-.omega.-hydroxyoctadecanoate; polyethylene glycol monostearate; and

poly(oxy-1,2-ethanediyl)-.alpha.-(1-oxooctadecyl)-.omega.-hydroxy-polyethyl ene glycol monostearate

The film former polymeric materials used in accordance with the present invention include anionic polymers, cationic polymers and neutral polymers.

I. Anionic Polymers

The anionic polymers carry negative charges in the ionized form and are capable of binding to cell surfaces mainly by electrostatic forces. Suitable anionic polymers include the following: ##STR16## wherein R is the polymeric chain; ##STR17## are anionic ligands; and M.sup.++ is a divalent cation.

Specific anionic polymers useful in the practice of the present invention include:

(1) Sulfated polysaccharides of the formula: ##STR18## wherein R is

  ______________________________________                                    
     3,6-anhydro-D-galactose linked                                            
                          (kappa carrageenan)                                  
     through C-4 to D-galactose;                                               
     .alpha.-D-galactose units (1-3) linked;                                   
                          (lambda carrageenan)                                 
     D-galactose          (iota carrageenan)                                   
     3,6-anhydro-D-galactose;                                                  
     D-galactose          (Agar--Agar)                                         
     3,6-anhydro-L-galactose:                                                  
     D-galactose          (Furcellaren)                                        
     3,6-anhydro-D-galactose;                                                  
     D-glucopyranose;     (Laminarin sulfate)                                  
     Galactan; and        (Galactan sulfate)                                   
     Galactosamino-glucuronans                                                 
                          (Chondroitin sulfates);                              
     and                                                                       
     ______________________________________

M.sup.++ is Mg.sup.++, Ca.sup.++, Zn.sup.++, Ba.sup.++ or mixtures thereof.

(2) Carboxylated polysaccharides of the formula: ##STR19## wherein R is

  ______________________________________                                    
     D-galacturonoglycan; and                                                  
                             (Pectin)                                          
     anhydro-D-mannuronic acid                                                 
     and anhydro-L-guluronic acid                                              
                             (Algin)                                           
     residues; and                                                             
     ______________________________________

M.sup.++ is Mg.sup.++, Ca.sup.++, Zn.sup.++, Ba.sup.++ or mixtures thereof.

(3) Cellulose derivatives of the formulae: ##STR20## wherein R is an anhydroglucose residue;

R' is CH.sub.3, C.sub.2 H.sub.5 or C.sub.3 H.sub.7 ;

R" is CH.sub.3 or C.sub.2 H.sub.5 ; and

M.sup.++ is Mg.sup.++, Ca.sup.++, Zn.sup.++, Ba.sup.++ or mixtures thereof.

Examples of cellulose derivatives include: sodium ethylcellulose sulfate, sodium cellulose acetate sulfate and sodium carboxymethyl cellulose.

(4) Sulfated, sulfonated or carboxylated synthetic polymers of the formula: ##STR21## wherein R is an aliphatic or aromatic hydrocarbon, such as polystyrene, poly(sulfon) resin or carboxylated (poly) vinyl; and

M.sup.++ is Mg.sup.++, Ca.sup.++, Zn.sup.++, Ba.sup.++ or mixtures thereof.

II Cationic Polymers

The cationic polymers carry positive charges in the ionized form. Suitable polymers for practicing the present invention include: dermatan sulfate, keratosulfate, hyaluronic acid, heparin and chitin.

III Neutral Polymers

Neutral polymers having polarizable electrons such as oxygen, nitrogen, sulfur, fluoride, chloride, bromide and iodide are also suitable for practicing the present invention. In the presence of a cation, such as Mg.sup.++, Ca.sup.++, Zn.sup.++ or Ba.sup.++, the polymers are partially polarized thereby providing intermolecular interactions between the polymer and the intestinal wall. Examples of these polymers include:

(a) Polysaccharides, such as starch, glycogen, glucan, fructans, mannans, galactomannas, glucomannas, galactans, xylans, glycuranans, dextran and starch amylose;

(b) Cellulose derivatives, such as methylcellulose, hydroxyethylcellulose, ethylhydroxyethyl cellulose, hydroxypropyl methylcellulose and hydroxypropyl cellulose; and

(c) Synthetic polymers, such as polyvinylpyrrolidone, polyvinyl alcohol and ethylene oxide polymers.

The dosages of the contrast agent used according to the method of the present invention will vary according to the precise nature of the contrast agent used. Preferably, however, the dosage should be kept as low as is consistent with achieving contrast enhanced imaging. By employing as small amount of contrast agent as possible, toxicity potential is minimized. For most contrast agents of the present invention dosages will be in the range of from about 0.1 to about 16.0 g iodine/kg body weight, preferably in the range of from about 0.5 to about 6.0 g iodine/kg of body weight, and most preferably, in the range of from about 1.2 to about 2.0 g iodine/kg body weight for regular X-ray visualization of the GI tract. For CT scanning, the contrast agents of the present invention will be in the range of from about 1 to about 600 mg iodine/kg body weight, preferably in the range of from about 20 to about 200 mg iodine/kg body weight, and most preferably in the range of from about 40 to about 80 mg iodine/kg body weight.

The concentration of the contrast agent should be in the range of from about 0.001% w/v to about 75% w/v of the formulation, preferably from about 0.05% w/v to about 50% w/v and most preferably of from about 0.1 % w/v to about 20% w/v.

The concentration of the film forming polymeric material depends on the particular polymer used, however, it should be in the range of 0.001 to about 15% w/v or higher in combination with a divalent substance, such as calcium lactate, having a concentration range of 0.001 to 15% W/V. Dosage level of the polymeric material may be in the range of from about 2 to about 15 g/kg body weight or higher.

The compositions of the present invention possess very good adherence to the walls of the gastrointestinal tract by forming an essentially uniform coating thereon.

The invention having been fully described, it will be apparent to one skilled in the art that changes and modifications can be made thereto without departing from the spirit and scope thereof.

Claims

1. An x-ray contrast composition designed for depositing a thin, flexible film membrane onto the mucosal lining of the nutrient absorbing inner surface of the intestine of a patient to form a barrier between said nutrient absorbing inner surface and the content of said intestine, said flexible film membrane to remain bound to said muscosal lining until eliminated by normal cell turnover comprising based on w/v:

(a) of from about 0.001 to about 15% of a polymeric material capable of forming a film membrane on the gastrointestinal tract in the pH range of from about 5 to about 8, and polymeric material being selected from the group consisting of
anionic polymers carrying negative charges in the ionized form, cationic polymers carrying positive charges in the ionized form, and neutral polymers, said neutral polymers having atoms containing polarizable electrons thereon selected from the group consisting of oxygen, nitrogen, sulfur, fluoride, chloride, bromide and iodide in combination with
(b) of from about 0.001 to about 15% of a divalent cation to potentiate the binding of said flexible film membrane to said mucusoal lining selected from the group consisting of Ca.sup.++, Mg.sup.++, Zn.sup.++ and Ba.sup.++; and
c) of from about 0.001 to about 75% of an x-ray contrast producing agent having the formula, or a pharmaceutically acceptable salt thereof ##STR22## wherein Z=H, halo, C.sub.1 -C.sub.20 alkyl, cycloalkyl, lower alkoxy, alkoxycarbonyl, cyano, where the alkyl and cycloalkyl groups can be substituted with halogen or halo-lower-alkyl groups;
R=C.sub.1 -C.sub.25 alkyl, cycloalkyl, ##STR23## or halo-lower-alkyl, each of which may be optionally substituted with halo, fluoro-lower-alkyl, lower-alkoxy, lower-alkoxy carbonyl or lower-alkoxy-carbonyloxy (CR.sub.1 R.sub.2).sub.p --(CR.sub.3 =CR.sub.4).sub.m Q, or (CR.sub.1 R.sub.2).sub.p --C.tbd.C--Q;
R.sub.1, R.sub.2, R.sub.3 and R.sub.4 are independently H or lower-alkyl, optionally substituted with halo;
x is 1-4;
n is 1-4;
m is 1-15;
p is 1-20; and
Q is H, lower-alkyl, lower-alkenyl, lower-alkynyl, lower-alkylene, aryl, or aryl-lower alkyl in an aqueous, pharmaceutically acceptable carrier.

2. The composition according to claim 1 wherein said x-ray contrast agent is selected from the group consisting of- bis-(4-iodophenyl) ether of polyethylene-glycol-400, 1,8-Bis-O-(2,4,6-triiodophenyl)-tripropylene glycol and 1,11-bis-(2,4,6-triiodophenoxy)-3,6,9-trioxaundecane.

3. The composition according to claim 1 wherein said x-ray contrast agent is selected from the group consisting of: 1,2-bis-(2,4,6-Triiodophenoxy)-ethane, bis-0-(2,4,6-triiodophenyl) ether of polyethylene glycol 40 and 1-(3-iodophenoxy)-3,6,9-trioxadecane.

4. The composition according to claim 1 wherein said x-ray contrast agent is selected from the group consisting of: 1,3-Bis-(2,4,6-Triiodophenoxy)-butane, 1-(3-iodophenoxy)-6-(2,4,6-triiodophenoxy)hexane and 1,12-Bis-(2,4,6-Triiodophenoxy)-dodecane.

5. The x-ray contrast composition of claim 1 wherein said polymeric material is anionic having the formula: ##STR24## wherein R is the polymeric chain; ##STR25## are anionic ligands; and M.sup.++ is a divalent cation.

6. The x-ray contrast composition of claim 5 wherein said anionic polymeric material is a sulfated polysaccharide having the formula: ##STR26## wherein R is

M.sup.++ is Mg.sup.++, Ca.sup.++, Zn.sup.++, Ba.sup.++ or a mixture thereof.

7. The x-ray contrast composition of claim 5 wherein said anionic polymeric material is a carboxylated polysaccharide having the formula: ##STR27## wherein R is

M.sup.++ is Mg.sup.++, Ca.sup.++, Zn.sup.++, Ba.sup.++ or a mixture thereof.

8. The x-ray contrast composition of claim 5 wherein said anionic polymeric material is a cellulose derivative of the formula: ##STR28## wherein H is an anhydroglucose residue;

R' is CH.sub.3, C.sub.2 H.sub.5 or C.sub.3 H.sub.7;
R" is CH.sub.3 or C.sub.2 H.sub.5; and
M.sup.++ is Mg.sup.++, Ca.sup.++, Zn.sup.++, Ba.sup.++ or a mixture thereof.

9. The X-ray contract composition of claim 8 wherein said cellulose derivative is selected from the group consisting of sodium ethylcellulose sulfate, sodium cellulose acetate sulfate and sodium carboxymethyl cellulose.

10. The x-ray contrast composition of claim 1 wherein said anionic polymeric material is a sulfated, sulfonated or carboxylated synthetic polymer having the formula: ##STR29## wherein R is an aliphatic or aromatic hydrocarbon; and

M.sup.++ is Mg.sup.++, Ca.sup.++, Zn.sup.++, Ba.sup.++ or a mixture thereof.

11. The x-ray contrast composition of claim 1 wherein said polymeric material is cationic selected from the group consisting of dermatan sulfate, keratosulfate, hyaluronic acid, heparin and chitin.

12. The x-ray contrast composition of claim 1 wherein said polymeric material is a polysaccharide.

13. The x-ray contrast composition of claim 12 wherein said polysaccharide is selected from the group consisting of starch, glycogen, glucan, fructans, mannans, galactomannans, flucomannas, galactans, xylans, glycuranans, dextran and starch amylose.

14. The x-ray contrast composition of claim 1 wherein said polymeric material is a cellulose derivative.

15. The x-ray contrast composition of claim 14 wherein said cellulose derivative is selected from the group consisting of methylcellulose, hydroxyethyl cellulose, ethylhydroxyethyl and hydroxypropyl cellulose.

16. The x-ray contrast composition of claim 1 wherein said polymeric material is polyvinylpyrrolidone, polyvinyl alcohol or an ethylene oxide polymer.

17. The x-ray contrast composition of claim 1 wherein said pharmaceutical carrier contains at least one surfactant.

18. The x-ray contrast formulation of claim 17 wherein said surfactant is cationic.

19. The x-ray contrast formulation of claim 17 wherein said surfactant is anionic.

20. The x-ray contrast formulation of claim 17 wherein said surfactant is zwitterionic.

21. The x-ray contrast formulation of claim 17 wherein said surfactant is nonionic.

22. The x-ray contrast formulation of claim 18 wherein said cationic surfactant is selected from the group consisting of cetyl trimethyl ammonium bromide and dodecyl dimethyl ammonium bromide.

23. The x-ray contrast formulation of claim 19 wherein said anionic surfactant is selected form the group consisting of sodium lauryl sulfate, sodium heptadecyl sulphate, an alkyl benzene sulphonic acid, sodium butylnaphthalene sulfonate and sulphosuccinate.

24. The x-ray contrast formulation of claim 1 wherein said nonionic surfactant is selected from the group consisting of carboxylic esters. carboxylic amides, ethoxylated alkylphenol and ethoxylated aliphatic alcohols, sorbitan esters, polyoxyethylene alkyl ethers and polyoxyethylene sorbitan fatty acid esters.

25. A method of carrying out x-ray examination of the gastrointestinal tract of a patient, said method comprises the oral or rectal administration to the patient the x-ray contrast composition of claim 1 and examining said gastrointestinal tract by an x-ray producing apparatus.

26. The method of claim 25 wherein said contrast agent is selected from the group consisting of: bis-(4-iodophenyl) ether of polyethylene-glycol-400, 1,8-Bis-O-(2,4,6-triiodophenyl)-tripropylene glycol and 1,11-bis-(2,4,6-triiodophenoxy)-3,6,9-trioxanundecane.

27. The method of claim 25 wherein said contrast agent is selected from the group consisting of: 1,2-bis-(2,4,6-Triiodophenoxy)-ethane, bis-O-(2,4,6-triiodophenyl)ether of polyethylene glycol 40 and 1-(3-Iodophenoxy)-3,6,9-trioxadecane.

28. The method of claim 25 wherein said contrast agent is selected from the group consisting of: 1,3-Bis-(2,4,6-Triiodophenoxy)-butane, 1-(3-iodophenoxy)-6-(2,4,6-triiodophenoxy)hexane and 1,12-Bis-(2,4,6-Triiodophenoxy)-dodecane.

29. The method of claim 25 wherein the amount of contrast agent administered to said patient is in the range of from about 0.1 to about 16.0 g iodine/kg body weight for regular x-ray visualization of the gastrointestinal tract.

30. The method of claim 25 wherein the amount of contrast agent administered to said patient will be in the range of from about 1 to about 600 mg iodine/kg body weight for CT scan visualization of the gastrointestinal tract.

31. The method of claim 25 wherein said pharmaceutically acceptable carrier contains at least one surfactant.

32. The method of claim 31 wherein said surfactant is cationic.

33. The method of claim 31 wherein said surfactant is anionic.

34. The method of claim 31 wherein said surfactant is zwitterionic.

35. The method of claim 31 wherein said surfactant is nonionic.

36. The method of claim 32 wherein said cationic surfactant is selected from the group consisting of cetyl trimethyl ammonium bromide and dodecyl dimethyl ammonium bromide.

37. The method of claim 33 wherein said anionic surfactant is selected from the group consisting of sodium lauryl sulfate; sodium heptadecyl sulphate, an alkyl benzenesulphonic acid, sodium butylnaphthalene sulfonate and sulphosuccinates.

38. The method of claim 35 wherein said nonionic surface active agent is selected from the group consisting of carboxylic esters, carboxylic amides, ethoxylated alkylphenols and ethoxylated aliphatic alcohols, sorbitan esters, polyoxyethylene alkyl ethers and polyoxyethylene sorbitan fatty acid esters.

39. The method of claim 25 wherein said polymeric material is anionic having the formula: ##STR30## wherein R is the polymeric chain; ##STR31## M.sup.++ is a divalent cation.

40. The method of claim 39 wherein said anionic polymeric material is a sulfated polysaccharide having the formula: ##STR32## wherein R is

M.sup.++ is Mg.sup.++, Ca.sup.++, Zn.sup.++, Ba.sup.++ or a mixture thereof.

41. The method of claim 39 wherein said anionic polymeric material is a carboxylated polysaccharide having the formula: ##STR33## wherein R is

M.sup.++ is Mg.sup.++, Ca.sup.++, Zn.sup.++, Ba.sup.++ or a mixture thereof.

42. The method of claim 39 wherein said anionic polymeric material is a cellulose derivative of the formula: ##STR34## wherein R is an anhydroglucose residue;

R' is CH.sub.3, C.sub.2 H.sub.5 or C.sub.3 H.sub.7;
R" is CH.sub.3 or C.sub.2 H.sub.5; and
M.sup.++ is Mg.sup.++, Ca.sup.++, Zn.sup.++, Ba.sup.++ or a mixture thereof.

43. The method of claim 42 wherein said cellulose derivative is selected from the group consisting of sodium ethylcellulose sulfate, sodium cellulose acetate sulfate and sodium carboxymethyl cellulose.

44. The method of claim 25 wherein said anionic polymeric material is a sulfated, sulfonated or carboxylated synthetic polymer having the formula: ##STR35## wherein R is an aliphatic or aromatic hydrocarbon; and

M.sup.++ is Mg.sup.++, Ca.sup.++, Zn.sup.++, B.sup.++ or a mixture thereof.

45. The method of claim 25 wherein said polymeric material is cationic selected from the group consisting of: dermatan sulfate, keratosulfate, hyaluronic acid, heparin and chitin.

46. The method of claim 25 wherein said polymeric material is a polysaccharide.

47. The method of claim 46 wherein said polysaccharide is selected from the group consisting of starch, glycogen, glucan, fructans, mannans, galactomannans, fluocmannas, galactans, xylans, glycuranans, dextran and starch amylose.

48. The method of claim 25 wherein said polymeric material is a cellulose derivative.

49. The method of claim 48 wherein said cellulose derivative is selected from the group consisting of methylcellulose, hydroxyethyl cellulose, ethylhydroxyethyl and hydroxypropyl cellulose.

50. The method of claim 25 wherein said polymeric material is polyvinylpyrrolidone, polyvinyl alcohol or an ethylene oxide polymer.

Referenced Cited
U.S. Patent Documents
2348231 May 1944 Strain et al.
2622100 December 1952 Newbery et al.
2832722 August 1958 Singher
3360436 December 1967 Felder et al.
3361700 January 1968 Archer et al.
3366625 January 1968 Hebky et al.
3553259 January 1971 Felder et al.
3574718 April 1971 Bjork et al.
3795698 March 1974 Soulal et al.
3825591 July 1974 Felder et al.
3883578 May 1975 Gries
4038379 July 26, 1977 Elinov et al.
4069306 January 17, 1978 Rothman
4120946 October 17, 1978 Quemille et al.
4588574 May 13, 1986 Felder et al.
5019370 May 28, 1991 Jay et al.
Foreign Patent Documents
1259565 September 1989 CAX
1481943 May 1967 FRX
55-127322 October 1980 JPX
Other references
  • Wang et al. Yaoxne Xuebao 16(8): 610-617 (1981). James, A M et al. Pharmaceutica Acta Helvetiae 47:244-55 (1972).
Patent History
Patent number: 5310538
Type: Grant
Filed: Mar 11, 1993
Date of Patent: May 10, 1994
Assignee: Sterling Winthrop Inc. (Malvern, PA)
Inventors: Edward R. Bacon (East Greenbush, NY), Carl R. Illig (Phoenixville, PA), Thomas J. Caulfield (Audubon, PA), Brent D. Douty (Coatesville, PA), Kurt A. Josef (Clifton Park, NY)
Primary Examiner: Gary E. Hollinden
Attorneys: Arthur Rosenstein, Imre Balogh
Application Number: 8/29,484