Abstract: In order to discriminate minute particles such as biological particles or organic polymers, a suspension of minute particles is formed into a particle stream with the individual particles substantially separated from each other, the particle stream is irradiated with a high-intensity light beam, the particle stream irradiated with the high-intensity light beam is formed into droplets, and the particle in each droplet is discriminated in accordance with the intensity of light emited from the particle upon irradiation with the high-intensity light beam. In discriminating the particles, a high-intensity light pulse is used as the high-intensity light beam, and each minute particle is discriminated in accordance with a change in the emitted light intensity over a period of time.

Abstract: Bodies such as nuclear fuel pellets are formed into a stack in a stacking lane (34) and the length of the stack is measured at intervals by device (40) to determine if the partly-formed stack is drifting away from a target length. Pellets are issued into the stacking zone (34) by a selector mechanism (32), the input of which receives pellets from three lanes (24, 26, 28) containing oversize, undersize and intermediate size pellets and the pellets issued to the stacking lane (34) are selected under the control of microprocessor-based unit (18) according to the extent of any drift from the target value. The pellets in lanes (24, 26, 28) are derived from a common supply (12) following segregation by a diverter mechanism (22) controlled by unit (18) in accordance with length measurements made by measuring device (16).

Abstract: A method for separating diamonds from gangue in which discrete units of gangue are passed through a beam of laser radiation capable of causing Raman spectral activation. The scattered radiation is detected and caused to actuate an ejector which separates units of diamond containing gangue from units of non-diamond containing gangue. The units of diamond containing gangue are then collected together.

Abstract: A particle separator for sorting particles suspended in a liquid according to certain characteristics, including a method of and apparatus for detecting a change in the droplet breakoff point of a liquid jet stream which is subjected to vibrations. The vibrations produce amplitude undulations on the surface of the jet stream. The amplitude of the undulations is monitored or interrogated at a fixed point on the jet stream prior to the breakoff point. A change in amplitude of the undulations at that fixed point produces a signal voltage at a masked sense diode the value of which is proportional to the amplitude change. This signal voltage may be used (1) to alert the operator that a change has occurred in the point at which the jet stream is breaking up into droplets, (2) to automatically control the intensity of the vibrations for restoring the amplitude of undulation at that fixed point to its original state, or (3) to automatically disable the sorting portion of the apparatus.

Abstract: Method and apparatus for sorting individual particles into containers, such as the wells of a microculture tray, are disclosed. The particles may comprise, for example, individual biological cells which then may be cultured, cloned, analyzed, or the like. The particles are incorporated into a flow stream which is jetted from a nozzle which is vibrated in the direction of the stream to break the jet stream into discrete drops. A particle sensor senses particles in the stream, and a signal processor in response to the particle sensor output produces an output when the sensed particle is of the type to be sorted from the stream. A pulse charger, responsive to the signal processor, is included for charging the drops as they break from the vibrating jet stream. The drops pass between charged deflection plates where they are deflected an amount related to the drop charge. For individual cell sorting, the signal processor is in normal inopertive condition to prevent sorting of drops from the drop stream.

Abstract: A method of ore sorting includes distinguishing ore objects or ore containing objects by the light reflected from a laser beam arranged to scan across each of the objects to be sorted. Where the surface of the object is transmitting to the light a halo is produced by certain objects caused by internal scattered reflections of the light. By monitoring the occurrence or degree of occurrences of halos clean distinction of respective objects can be made. In a particular application, the identification of the presence of quartz pebbles in rock specimens can be used for detecting and sorting gold containing objects.

Abstract: An apparatus for sorting particles comprises a nozzle or the like for forming discrete droplets of particles from a flowing liquid stream containing those particles. A detector is provided for detecting particles in the stream. A charging electrode, responsive to the detector, charges some droplets of selective interest with a positive electrical charge and some with a negative electrical charge. Electrical deflecting plates deflect charged droplets of selective interest. Droplets of selective interest, charged with opposite polarities, are collected in a collection well inside which the electrical charge is substantially neutralized. A method for sorting particles, such as cells or the like, substantially in accordance with the above-described apparatus is another aspect of the present invention.

Abstract: An optical inspection system is disclosed which determines surface and other features of objects by illuminating them with a light beam having substantially uniform light intensity in space and time. The light beam is derived from a light source whose light intensity may vary in space and time. The surface features of each object are inspected by detecting light reflected from the object through a lens system which focuses the reflected light on an array of photosensitive elements. The output signals provided by the array may be used to provide a measure indicative of the conformance of the inspected surface to predetermined criteria. The inspection system is further capable of determining other features of the inspected object, such as the height of the object, by illuminating the object with a second light beam derived from a second light surface.

Abstract: An automated inspection station comprises a pair of rotating rollers for supporting a collected group of pellets. A pusher drops in behind the group to urge the entire group forward. The rotating rollers cause the entire group of pellets to spin in unison as the pellets pass through an optical inspection station. The optical inspection station comprises a light source, diverging and collimating lenses to illuminate each rotating pellet in turn. Signals are generated by at least two arrays of photodiodes in response to light reflected from the surface of the pellets. Circuitry normalizes the signals of the respective diodes for variations unrelated to pellet surface features. The signals are analyzed to ascertain conformance of each pellet to length, cylindricality diameter and surface reflectivity requirements. A pellet sorter diverts unacceptable pellets from the path, while the acceptable pellets are transported to a collection station.

Abstract: A particle separator for sorting particles suspended in a liquid according to certain characteristics, including a method of and apparatus for detecting a change in the droplet breakoff point of a liquid jet stream which is subjected to vibrations. The vibrations produce amplitude undulations on the surface of the jet stream. The amplitude of the undulations is monitored or interrogated at a fixed point on the jet stream prior to the breakoff point. A change in amplitude of the undulations at that fixed point produces a signal voltage the value of which is proportional to the amplitude change. This signal voltage may be used (1) to alert the operator that a change has occurred in the point at which the jet stream is breaking up into droplets, (2) to automatically control the intensity of the vibrations for restoring the amplitude of undulation at that fixed point to its original state, or (3) to automatically disable the sorting portion of the apparatus.

Abstract: Control for the operation of a mechanical handling and gauging system for nuclear fuel pellets. The pellets are inspected for diameters, lengths, surface flaws and weights in successive stations. The control includes, a computer for commanding the operation of the system and its electronics and for storing and processing the complex data derived at the required high rate. In measuring the diameter, the computer enables the measurement of a calibration pellet, stores that calibration data and computes and stores diameter-correction factors and their addresses along a pellet. To each diameter measurement a correction factor is applied at the appropriate address.The computer commands verification that all critical parts of the system and control are set for inspection and that each pellet is positioned for inspection. During each cycle of inspection, the measurement operation proceeds normally irrespective of whether or not a pellet is present in each station.

Abstract: Disclosed are a method and a device for distinguishing particles or regions therein to which fluorochrome-labeled antibodies or antigens, termed conjugates, are bound by the specific immune reaction of an antibody with its homologous antigen from particles or regions to which the conjugates are nonspecifically bound. The method is based on the different bleaching behavior caused by the different types of binding. Use is made of a microscope whose entire field of view is conventionally illuminated, a central laser beam serving for bleaching analysis.

Abstract: A system for non-destructive, non-contacting use for detecting imperfections in multi-layered supply rolls of wound plastic film comprises a laser which scans the length of the particular roll being inspected with a photo-electric detector at a fixed reference angle which picks up reflected unimpeded and scattered light plus associated indicating and/or recording equipment. Defects in the roll, particularly those related to the upper longitudinal surface thereof, cause light scattering which decreases the signal strength to the indicator or recorder, thus noting the presence of a wrinkle or the like imperfection in the film roll.

Abstract: A fluidic assembly for an ultra-high-speed chromosome flow sorter using a fluid drive system, a nozzle with an orifice having a small ratio of length to diameter, and mechanism for vibrating the nozzle along its axis at high frequencies. The orifice is provided with a sharp edge at its inlet, and a conical section at its outlet for a transition from a short cylindrical aperture of small length to diameter ratio to free space. Sample and sheath fluids in separate low pressure reservoirs are transferred into separate high pressure buffer reservoirs through a valve arrangement which first permit the fluids to be loaded into the buffer reservoirs under low pressure. Once loaded, the buffer reservoirs are subjected to high pressure and valves are operated to permit the buffer reservoirs to be emptied through the nozzle under high pressure.

Abstract: Apparatus for inspecting nuclear fuel pellets in a sealed container for diameter, flaws, length and weight. The apparatus includes, in an array, a pellet pick-up station, four pellet inspection stations and a pellet sorting station. The pellets are delivered one at a time to the pick-up station by a vibrating bowl through a vibrating linear conveyor. Grippers each associated with a successive pair of the stations are reciprocable together to pick up a pellet at the upstream station of each pair and to deposit the pellet at the corresponding downstream station. The gripper jaws are opened selectively depending on the state of the pellets at the stations and the particular cycle in which the apparatus is operating. Inspection for diameter, flaws and length is effected in each case by a laser beam projected on the pellets by a precise optical system while each pellet is rotated by rollers.

Abstract: An improved method of sorting biological cells in a conventional cell sorter apparatus includes generating a fluid jet containing cells to be sorted, measuring the distance between the centers of adjacent droplets in a zone thereof defined at the point where the fluid jet separates into descrete droplets, setting the distance between the center of a droplet in said separation zone and the position along said fluid jet at which the cell is optically sensed for specific characteristics to be an integral multiple of said center-to-center distance, and disabling a charger from electrically charging a specific droplet if a cell is detected by the optical sensor in a position wherein it will be in the neck area between droplets during droplet formation rather than within a predetermined distance from the droplet center.

Abstract: A novel method is disclosed for detecting and controlling the flow rate of a perturbed, droplet-forming stream in an electrostatic particle sorting apparatus. Detection apparatus is located at two points along the stream, at a first particle sensing point for sensing the presence of particles within a core portion of the stream, and at a second downstream point, preferably the stream breakpoint, for sensing light scatter and extinction characteristics which are proportional to the surface characteristics of the sheath portion of the stream. Changes in phase shift, pulse width, duty cycle, pulse area, or breakpoint location are detected by analyzing these sheath surface-related characteristics. An error signal is produced in response to such changes which drives an electromechanical fluid flow regulator to increase or decrease the fluid flow rate in a direction which tends to minimize the error signal. The flow rate is thus maintained at a reference flow rate setting.

Abstract: A novel method for positioning the point of droplet formation in the jetting fluid of an electrostatic sorting device is disclosed. The formation of the uniform droplets is carried out via the application of vibrational energy inparted by a transducer to a jetting laminar flow stream. Previously sensed particles contained within the core portion of the laminar stream are subsequently sorted from the stream as part of a subsequently formed droplet. The disclosed method uses the dependency of the droplet formation point on the amplitude of the wave form applied to the transducer and the modulation of this amplitude to control the droplet formation point distance. The position of the droplet formation point is detected via the use of a light source and photodetector focused on the jetting stream at the position at which the droplet formation point is to be located, and a method for automatically maintaining the breakpoint at that position is described.

Abstract: In order to initiate the charging of droplets at a specific point in time with respect to the formation of those droplets in an electrostatic flow sorting system, the charge phase of the system of the present invention is continuously and automatically adjusted by droplet break point monitoring. The charge phase is therefore flow rate and fluid velocity independent. The break point is monitored by a light source which is focused onto the jet stream at the droplet formation break point, or close to it, and the modulation of light caused by the scatter induced by the sheath stream portion as it passes the focused beam of light is detected via the use of a suitable electro-optical detector. From the output of the electro-optical detector, after appropriate signal conditioning, an electrical wave form is obtained which shape depends upon the shape of the fluid column passing the focus beam.

Abstract: A method for the measurement of fluid velocity and fluid flow rate is described which may be applied to a system in which fluid is caused to exit through an orifice to form a free jet. Optical as well as fluidic properties of the jetting fluids are used to determine the mean velocity of the jet between its point of exit from the orifice and at least a preselected detection point. From the mean velocity determination, the fluid flow rate may also be determined. A marker signal perturbation is applied to the jetting fluid at the orifice, and is detected downstream at a stream sensing point which is located a preselected distance from that orifice.

Abstract: A novel method and apparatus for automatically setting the time delay for relatively charging droplets in an electrostatic particle sorting system is disclosed. By utilizing a movable sheath sensing means for sensing the light scatter and extinction of this stream, the flow rate and distance to breakpoint are automatically measured and used to calculate and set the timing delay circuitry. This insures that droplet charging at the breakpoint will occur synchronously to produce selective relative charging of those droplets containing particles to be sorted.

Abstract: A novel method and system are disclosed for detecting the breakpoint of a perturbed flow stream. The scattering and extinction of light caused by a perturbed stream at a preselected flow stream sensing point therealong is measured to produce a real time waveform proportional thereto. The real time waveform is differentiated to produce a differentiated waveform, which is then selectively monitored to distinguish a waveform exhibiting substantially three (positive and negative) peaks per cycle, whereby such detection indicates that at least a portion of the breakpoint region of said flow stream corresponds with said flow stream sensing point. In order to align the stream sensing point with respect to a preselected point within the breakpoint region of the flow stream, the breakpoint region of the stream is caused to move with respect to the stream sensing point until the breakpoint pulse width of one of the peaks corresponds with a preselected pulse width reference.

Abstract: A method and apparatus for forming a separate single drop wherein a fluid stream is formed in air and an electrical charge or radiant energy is applied to the stream at a particular location in order to disturb the stream and create a single drop. The drop may be formed surrounding a particle and the drop may be charged when formed, then deflected for separate collection.

Abstract: A method and apparatus for forming a separate single drop wherein a fluid stream is formed in air and an electrical charge or radiant energy is applied to the stream at a particular location in order to disturb the stream and create a single drop. The drop may be formed surrounding a particle and the drop may be charged when formed, then deflected for separate collection.

Abstract: This invention relates to the inspection of glass ampules to determine whether or not a complete score line is present. A laser beam is directed to the point on the ampule where a score line would be located. The laser beam is reflected in a diffuse or specular fashion depending upon whether or not a score line is present. By detecting the amount of light which is diffusely reflected in a particular direction, determination of the presence of a score line is possible. The same inspection may be utilized to determine whether the constriction on an ampule is of the proper diameter.

Abstract: In the photometric sorting of objects each object is examined in each of a predetermined number of zones for the presence or absence of one or more predetermined surface characteristics. The invention provides that a characteristic determined in one zone be attributed to at least one other zone into which the object extends.