Abstract: Methods, devices, apparatus, and systems are provided for image analysis. Methods of image analysis may include observation, measurement, and analysis of images of biological and other samples; devices, apparatus, and systems provided herein are useful for observation, measurement, and analysis of images of such samples. The methods, devices, apparatus, and systems disclosed herein provide advantages over other methods, devices, apparatus, and systems.

Abstract: A method for detecting and controlling supply of an immersion medium into an immersion film region between the front lens of a microscope objective and a preparation, particularly in automated microscopes, using a light source which is reflected into the microscope beam path by means of a beam splitter. The state of the immersion film region is recorded and evaluated in a detection unit of the microscope or a detection unit of the autofocus device through an autofocus signal of the light source and/or a reflection of a modulation object arranged in the intermediate image plane of an incident light illumination, and is transmitted to a control unit for the purpose of a change or an error treatment of the immersion process.

Abstract: The focus control culture observation apparatus enable simple and reliable detection of a focusing position of an objective lens on a subject and with certainty. When a focusing position is detected, an area, which includes an edge portion of the culture medium drop is selected as an AF area which is to be a target of detecting the focusing position of the objective lens on the culture medium drop. The apparatus detects the focusing position on the culture medium drop based on an observation image of the AF area. By detecting the focusing position as a targeting area that is different from an area which is near the edge of the culture container and where illumination unevenness is generated, the detection accuracy can be improved.

Abstract: An apparatus which provides a multiplicity of test colors and neutral tones simultaneously within a microscope's optical system that may be used to align, and/or calibrate the microscope, and the light source and any associated recording devices, automatically or with input from an operator. The test colors and neutral tones may also be used as references against which to judge specimens being viewed in the microscope. Also disclosed is a method of operating a microscope using such a such pattern.

Abstract: A focusing device for an optical microscope may include a light emitting unit configured to emit laser light having a specific wavelength, a wedge mirror configured to enable the emitted laser light to be incident on a plurality of locations of a surface of a specimen, first and second light receiving units configured to detect an amount of laser light reflected from the surface of the specimen, a spatial filter configured to eliminate out-of-focus light from light beams reflected from the surface of the specimen and to detect an amount of in-focus light, and a control unit configured to generate a control signal used to carry out focus adjustment of the optical microscope using a plurality of light-amount information detected by the first and second light receiving units and the spatial filter.

Abstract: The present invention relates to an autofocus aperture stop (5, 6) in a triangulating autofocusing device (21) for a microscope (40), wherein the autofocus aperture stop (5, 6) comprises at least one diaphragm opening (3, 4) with which a measuring beam pencil (34) used for the autofocusing and running in the direction of the optical axis (18) of the autofocusing device (21) can be limited in its cross section, wherein the diaphragm opening (3, 4) of the autofocus aperture stop (5, 6) is arranged in a decentred position at a spacing from the optical axis (18) of the autofocusing device (21), wherein a decentred autofocus measuring beam (36) can be generated by the diaphragm opening (3, 4) in one half of the cross section (17) of the measuring beam pencil (34).

Abstract: The presently claimed invention provides a focusing apparatus and method for a fluorescence microscope which is capable of shortening the time in focusing, increasing system throughput and avoiding from undesirable photo-bleaching. The fluorescence microscope of the present invention employs a portion of excitation light to form images of a sample to determine a focus plane for fluorescence imaging. As intensity of the portion of the excitation light is much higher than that of fluorescence light, the exposure time is highly reduced for image formation used for focusing purpose. The fluorescence microscope of the present invention enables to perform both predictive focusing and multiplex focusing.

Abstract: A microscope system includes an accommodation unit, a stage, an optical system, an image pickup unit, a movement mechanism, a control unit, an image processing unit, and a display unit. The accommodation unit is capable of accommodating a plurality of specimens. On the stage, each of the specimens loaded from the accommodation unit is placed. The optical system includes a lens for spherical aberration correction. The image pickup unit is capable of capturing a partial image of each of the specimens placed on the stage, via the optical system. The movement mechanism moves the lens for spherical aberration correction along an optical axis. The control unit controls movement of the lens for spherical aberration correction by the movement mechanism and correct spherical aberration. The image processing unit combines the partial images captured by the image pickup unit and generate a composite image. The display unit displays the generated composite image.

Abstract: The invention relates to a method and to an arrangement for operating a dynamic nano focusing system for use thereof in the field of microscopy, interferometry or similar applications, wherein the nano focusing system comprises a lever-transmission piezo actuator and a frictionless guide based on a resiliently deformable solid body joint which is connected to a mounting unit, in particular for a lens, in order to implement the desired adjustment paths for the focusing. According to the invention, in order to increase the dynamics during the focusing process, a secondary fine adjustment movement is superimposed on the primary adjustment movement, said secondary fine adjustment movement having a smaller adjustment path but higher frequency than the primary adjustment movement, wherein, when the fine adjustment is carried out, a determination is made as to whether the focusing result changes, in order to specify according thereto the amount and/or the direction of the primary adjustment movement.

Abstract: A microscope with means for adjusting the focal range, comprising a first objective lens for transmitting the object light of an illuminated object in the direction of a detector, with a second objective lens being disposed in the direction of the light upstream of the detector, which second objective lens is followed by a first mirror that can be adjusted in the direction of the optical axis, with at least one second mirror for transmitting light from the first objective lens in the direction of the second objective lens and from the second objective lens to the detector being disposed in the optical path, which second mirror is a fully reflective mirror, or a microscope with means for adjusting the focal range, comprising a first objective lens for transmitting the object light of an illuminated object in the direction of a detector, with a second objective lens being disposed in the direction of light upstream of the detector, which second objective lens is followed by a first mirror that can be adjusted i

Abstract: According to one aspect, the present invention relates to an imaging system 100 for enhancing microscopic images of unstained cells. The imaging system 100 comprises a light source 102 for producing light 120a, a sample holder 109 for containing cells to be imaged, a condenser 104 for focussing the light 120b at a focal plane within the sample holder 109 on the cells to be imaged, a translation mechanism for moving the focal plane of the light 120b relative to the sample holder 109 and a detector system 112 configured to acquire a plurality of images at respective focal planes within the sample holder 109 and process the plurality of images to provide an enhanced processed imaged.

Abstract: A focus control apparatus and method capable of generating a focus position signal according to a real focus position error even in proximity to the interference between regions having different thicknesses to perform a high precision focus position control using the focus position signal are provided.

Abstract: A device for focusing a microscope objective on a sample accurately with a high spatial resolution. The device has a positioning unit having a main body, an objective holder movably supported on the main body and adapted to hold the microscope objective, and an actuator for moving the objective holder along the optical axis of the microscope objective. The objective holder holds the microscope objective only at a front portion of the microscope objective facing the sample. The positioning unit includes one or more lever arms, each of which coupled at its one end via a first flexure bearing to the main body and at its other end via a second flexure bearing to the objective holder. The main body of the positioning unit is attached to a stage that carries the sample.

Abstract: It is possible to generate image data of a biological tissue in a short time. A focus information generating device (2) receives a first reflected light beam (Lr1) split from a reflected light beam (Lr) and a second reflected light beam (Lr2) passing through a pin-hole plate (36). A signal processing unit (13) calculates a uniform reflectance (RE) representing a light amount ratio of the second reflected light beam (Lr2) to the first reflected light beam (Lr1) along with a sum signal (SS) and a difference signal (SD). An integrated control unit (11) detects a position (Z1) corresponding to a top surface (104A) of a cover glass (104) on the basis of the sum signal (SS) and the difference signal (SD) and detects a position (Z3) representing a biological tissue (102) on the basis of the sum signal (SS) and the uniform reflectance (RE).

Abstract: A microscope system and method empirically determines the boundaries of the depth of field of an objective lens. The system and method are largely automated, with the manipulation of a specimen to be imaged being carried out by processors and associated equipment. Calculations of the empirical depth of field are also likewise automated. Upon empirically determining the boundaries of the depth of field, the specimen, particularly when transparent or translucent, can be accurately imaged at user-defined depths smaller than the depth of field.

Abstract: A surgical microscope objective has an adjustable focal intercept and defines an optical axis. The objective has an objective body wherein a negative member is fixed and in which a positive member can be displaced. The negative member faces toward the object and has a lateral edge. The positive member is held in a frame, which is guided on a first guiding section formed on the objective body so it can move in the direction of the optical axis using a first guiding part. The positive member is guided on a second guiding section, which is formed on the objective body, using a second guiding part connected to the frame of the positive member and movable between the lateral edge of the negative member and the objective body. The second guiding part is offset from the first guiding part in the direction of the optical axis facing the negative member.

Abstract: A microscope focusing mechanism includes a fixing part attached to a microscope main body, a fixing-side guide member attached to the fixing part, a plate-shaped base part that supports at least one of a stage for placing a specimen and a revolver to which multiple objective lenses are detachably attached, a moving-side guide member that is fixed to the base part, that is connected to the fixing-side guide member, and that is movable in a direction of an optical axis of one of the objective lenses, and a plate member that is arranged on a side of the base part opposite to a side where the moving-side guide member is arranged. A linear expansion coefficient of a material of each of the moving-side guide member and the plate member differs from a linear expansion coefficient of a material of the base part.

Abstract: A method for autofocusing in microscopic examination of a specimen located at the focus of a microscope objective uses an autofocus beam path, the autofocus beam path being directed, via a deflection device arranged on the side of the microscope objective facing away from the specimen, toward the microscope objective, and from there onto a reflective autofocus interface in the specimen region. The autofocus beam path is reflected at the autofocus interface and directed via the microscope objective and the deflection device toward an autofocus detector. The deflection device comprises two regions spaced apart from one another in a propagation direction of the autofocus beam path. Each region reflects the autofocus beam path. The autofocus detector is arranged in a plane conjugated with the microscope objective pupil to acquire an interference pattern. The focus of the microscope is adjusted as a function of the acquired interference pattern.

Abstract: A system includes a displacement sensor, an actuator connected to the displacement sensor, and a feedback unit. The displacement sensor is configured to measure at least one of a relative position and a relative orientation between the displacement sensor and the target object. The feedback unit receives a signal from the displacement sensor related to the measured relative position or relative orientation and controls the actuator to move the displacement sensor on the basis of variations in the received signal arising due to a change in environmental conditions.

Abstract: An imaging device includes: a first imaging unit which does not include a phase difference detecting pixel on an imaging element; a second imaging unit which includes a phase difference detecting pixel on an imaging element; a pixel comparing unit which compares first obtained image obtained by the first imaging unit with a second obtained image obtained by the second imaging unit; a correcting unit which corrects phase difference information obtained by the second imaging unit based on a comparison result by the image comparing unit; a phase difference applying unit which applies the phase difference information corrected by the correcting unit to the first obtained image; and a recording unit which records image information.

Abstract: Disclosed herein is a microscope, including: an illumination optical system; a first image creation optical system; a second image creation optical system; an illumination-field-diaphragm focus adjustment section; and a characteristic-quantity computation block, wherein the illumination-field-diaphragm focus adjustment section adjusts the image creation position for the illumination field diaphragm on the basis of the characteristic quantity computed by the characteristic-quantity computation block.

Abstract: The present invention relates to an afocal zoom system for a microscope with a shutter for controlling the depth of focus of the microscopic image produced by an object, wherein at least one shutter is disposed in front of the first lens group of the zoom system, viewed from the object, in the direction of the beam path passing through the zoom system, and/or at least one shutter is disposed on a lens group of the zoom system, the diameter of which can be varied in order to control the depth of focus, without causing vignetting of the edge beams.

Abstract: A focusing apparatus for use with an optical system having a high NA objective lens includes an image forming and capturing mechanism for forming an image in an intermediate image zone and for capturing an image by receiving and refocusing light from a selected focal plane within the intermediate image zone, and a focus adjusting mechanism for adjusting the position of the selected focal plane within the intermediate image zone. The image forming and capturing mechanism includes at least one high NA lens. In use, spherical aberration introduced by the high NA objective lens is reduced.

Abstract: A microscope system and an autofocus method capture a subject at a specified time interval through an optical image forming system while relatively moving a stage loaded with the subject and the optical image forming system of the microscope; calculate a contrast value indicating a high and low contrast of a captured observation image; detect a maximum contrast image whose calculated contrast value is maximum in a plurality of performed observation images; detect the relative position of the stage and the optical image forming system; perform a correcting process of moving the detected relative position corresponding to the detected maximum contrast image by a specified value in a direction opposite the direction of the relative movement; and bring focus using the corrected relative position as a focal point.

Abstract: The focusing operation of a microscope, control rotary knobs which receive the motion of fingers or hand of an operator to change the focus position relative to a sample under observation. Two of the control rotary knobs are on a common shaft and coaxial with one another and rotationally fixed with respect to one another, and are connected to a position encoder. A toggle switch which is connected to the control circuit and can be actuated by touching or moving one or both rotary control knobs. Whereby the drive circuit is configured such that it generates, as a function of the rotation of the two rotary knobs and as a function of the actuation or position of the toggle switch, a rough or fine adjustment of the focus position relative to the sample under observation.

Abstract: The present invention relates to a microscopic image capturing apparatus having a structure that, in scanning an imageable area of an imaging unit in a predetermined direction in an imaging object area, in which a sample is present, can reliably set a focal point of the imaging unit on each imaging position set inside the imaging object area regardless of the type of focusing actuator. The microscopic image capturing apparatus has a sample setting stage having a sample setting surface that is inclined with respect to a scan plane orthogonal to an optical axis of an objective lens. By moving the sample setting stage along the scan plane such that the distance in the optical axis direction between the imaging unit and the sample setting surface varies monotonously, the focal point position of the imaging unit is adjusted in only one direction along the optical axis of the objective lens.

Abstract: Methods and apparatus (1100) for trapping fluid-borne object(s) (212) using one or more Fresnel zone plates (202) located in proximity to a fluid medium (208). Optical tweezers based on one or more Fresnel zone plates may be integrated with a microfluidic structure (e.g., chambers, channels) (1104) of various geometries so as to form one or more optical traps (215) within a fluid medium contained by the microfluidic structure(s). Three-dimensional trapping of objects can be obtained with stiffness comparable to that of conventional optical tweezers based on a microscope objective. In one example, a single Fresnel zone plate is particularly configured to form multiple optical traps upon irradiation, so as to trap multiple objects simultaneously. Exemplary applications of the methods and apparatus disclosed herein include determination of various fluid medium properties (e.g., velocity, refractive index, viscosity, temperature, pH) and object sorting.

Abstract: A microscope includes a member supported for movement relative to a base along a path of travel, and optics on the member for imaging a region that includes a location for a specimen. The method and apparatus involve: effecting relative movement of a cam operatively coupled to one of the member and the base with respect to a cam follower operatively coupled to the other of the member and the base in a manner so that the cam follower slides along a cam surface on the cam; and responding to the sliding movement of the cam follower along the cam surface by effecting relative movement of the cam and cam follower in a manner that in turn effects movement of the member along the path of travel relative to the base.

Abstract: An autofocus apparatus and a method achieve a higher level of speed and robustness, and are particularly suited for fluorescence microscopy of biological samples, automated microscopy and scanning microscopy. A high speed is achieved via a light pattern in the sample, detected spatially resolved by a detector generating at least two signals corresponding to a reflex pattern of the light pattern. The two signals are subtracted generating a positioning signal and the focus of the objective in the sample is adjusted depending on the positioning signal.

Abstract: A photographing control unit comprising a drive signal generator, an analog front end, a synchronization signal generation unit, and a system control unit controls an imager under a predetermined photographing condition, so that the imager photographs an observation image of a sample observed by a microscope and formed on a light receiving surface of the imager. A computing unit obtains inputs of a plurality of photographing parameters, determines a photographing condition based on the obtained photographing parameters, and sets the photographing condition for the photographing control unit. A display unit displays the designable ranges of the photographing parameters. Here, when the computing unit obtains the input of a priority parameter, it changes the designable range of the plurality of photographing parameters other than the priority parameter based on the priority parameter and causes the display unit to display the designable range after the change.

Abstract: A multi-mode optical fiber delivers light from a radiation source to a multi-focal confocal microscope with reasonable efficiency. A core diameter of the multi-mode fiber is selected such that an etendue of light emitted from the fiber is not substantially greater than a total etendue of light passing through a plurality of pinholes in a pinhole array of the multi-focal confocal microscope. The core diameter may be selected taking into account a specific optical geometry of the multi-focal confocal microscope, including pinhole diameter and focal lengths of relevant optical elements. For coherent radiation sources, phase randomization may be included. A multi-mode fiber enables the use of a variety of radiation sources and wavelengths in a multi-focal confocal microscope, since the coupling of the radiation source to the multi-mode fiber is less sensitive to mechanical and temperature influences than coupling the radiation source to a single mode fiber.

Abstract: A microscope system that is capable of changing a status of observation of a sample comprises an instruction unit for giving instruction for driving one or more optical members including an objective lens or for changing a relative position of the sample and the objective lens; and an image capturing unit for capturing an observed image of the sample as a still image or a live image. The microscope system changes an order for performing operations in accordance with the instruction from the instruction unit, the operations including an operation of driving the one or more optical members or changing the relative position; an operation of switching the illumination light for the sample from being cut-off or reduced to being applied; and an operation of switching the image displayed in a display unit from the still image to the live image.

Abstract: A focus detection apparatus is provided with a light source 16 that emanates light with a given wavelength range; a reflection member 15 that reflects light emanated from the light source 16 to lead to an object 6a, reflects light from the light source 16 reflected from the object 6a, and transmits light from the object 6a with at least two different wavelength ranges except the light reflected from the object 6a; a photodetector 21 detecting the light from the light source 16 reflected from the object 6a; and a controller 22 detecting a focus shift between the objective lens 8 and the object 6a in the microscope 2 based on a signal detected by the photodetector 21, thereby providing a focus detection apparatus capable of limiting wavelength range of light for focus detection thereby able to use wider wavelength range for the microscope observation, and a microscope equipped therewith.

Abstract: A device for focusing a microscope objective on a sample accurately with a high spatial resolution. The device has a positioning unit having a main body, an objective holder movably supported on the main body and adapted to hold the microscope objective, and an actuator for moving the objective holder along the optical axis of the microscope objective. The objective holder holds the microscope objective only at a front portion of the microscope objective facing the sample. The positioning unit includes one or more lever arms, each of which coupled at its one end via a first flexure bearing to the main body and at its other end via a second flexure bearing to the objective holder. The main body of the positioning unit is attached to a stage that carries the sample.

Abstract: Microscopy methods and apparatus in which one or more microfabricated optical elements (e.g., one or more Fresnel zone plates) operate as one or an array of objective lenses. A single object or a plurality of objects may be scanned in parallel. A single, low-numerical-aperture relay optic can be used with the one or more optical elements eliminating the need for one or more confocal pinhole apertures. When an array of optical elements is used, hundreds to thousands of objects can be imaged or inspected simultaneously onto a two-dimensional imaging device, such as a CCD array. The microfabricated optical elements can be readily configured for imaging with a solid immersion medium. Imaging resolutions on the order of one wavelength of the illumination source, and less, can be achieved.

Abstract: An optical system includes a dynamically focused lens that variably changes focal length to focus light on a material under evaluation at discrete axial depths, and a scanning element that laterally scans the material to focus light on the material at points that are laterally adjacent the axial depth.

Abstract: In a biological specimen imaging method, a biological specimen which is stored in a storing section of a substrate having plural storing sections and emitting a feeble light is imaged through an objective lens. The biological specimen imaging method includes moving any one of the substrate and the objective lens or both until the desired storing section falls within the field of view of the objective lens, measuring any one of a focal position at a near point and the focal position at a far point of the objective lens or both, determining the focal position of the objective lens focused on an observed target region in the biological specimen stored in the desired storing section based on the measured focal position, and adjusting the focal position of the objective lens to the determined focal position so as to image the biological specimen through the objective lens.

Abstract: A 3-D optical microscope, a method of turning a conventional optical microscope into a 3-D optical microscope, and a method of creating a 3-D image on an optical microscope are described. The 3-D optical microscope includes a processor, at least one objective lens, an optical sensor capable of acquiring an image of a sample, a mechanism for adjusting focus position of the sample relative to the objective lens, and a mechanism for illuminating the sample and for projecting a pattern onto and removing the pattern from the focal plane of the objective lens. The 3-D image creation method includes taking two sets of images, one with and another without the presence of the projected pattern, and using a software algorithm to analyze the two image sets to generating a 3-D image of the sample. The 3-D image creation method enables reliable and accurate 3-D imaging on almost any sample regardless of its image contrast.

Abstract: Apparatus and methods are provided which allow the rapid collection of image data in situations where ancillary equipment must be controlled and co-ordinated as part of an image formation process, such as confocal microscopy for example. The apparatus includes control means (20?) for co-ordinating the operation of the apparatus, and operable to receive a first trigger signal (33, 33?, 37) indicating the completion of an operation from one component and transmit a second trigger signal (27, 27?) to start an operation by another component in response to the first trigger signal. Such a configuration is operate to reduce delays encountered in operation of the apparatus.

Abstract: A focusing apparatus for use with an optical system having a high NA objective lens includes an image forming and capturing apparatus for forming an image in an intermediate image zone, and for capturing an image by receiving and refocusing light from a selected focal plane within the intermediate image zone, and a focus adjusting apparatus for adjusting the position of the selected focal plane within the intermediate image zone. The image forming and capturing apparatus includes at least one high NA lens. In use, spherical aberration introduced by the high NA objective lens is reduced.

Abstract: An objective changer for a telescope-type stereomicroscope which has two telescope systems whose entrance-side beam paths are oriented parallel to one another, which includes a carrier and an objective holder which is held at the carrier so as to be movable relative to the carrier between a first stereoscopic working position and a second stereoscopic working position and a third working position. The objective holder has at least a first and a second receptacle for stereo objectives and a third receptacle for an additional objective. The two telescope system beam paths penetrate one of the receptacles for the stereo objectives at least in the stereoscopic working positions when the objective changer is arranged at the stereomicroscope.

Abstract: The invention is based on an autofocus method in which light from a light source (12) is focused at a measurement light focus (52) in a sample (6) and is reflected from there and the reflected light is guided through an optical system (22) in two light paths (48, 50) onto at least two detector elements (72, 74). In order to achieve fast and accurate automatic focusing on the sample, it is proposed that the measurement light focus (52) is moved in layers of the sample (6) which reflect light to different extents, and the detector elements (72, 74) are arranged in such a way that, in this case, profiles of a radiation property registered by the detector elements (72, 74) are different and a focus position is set in a manner dependent on the profiles.

Abstract: A microscope image pickup system is one having a microscope apparatus enabled to change an observation state by driving one or more optical members, which comprises: an image pickup unit for picking up an image of an observation object; an image process unit for applying an image process to an image picked up by the image pickup unit; an input unit for inputting a process factor of the image process unit, wherein an observation state of the microscope apparatus and/or an image pickup condition of the image pickup unit are set up and an image is picked up so as to pick up an image of an image quality being equal to, or better than, that of an image to which an image process is applied on the basis of the process factor input from the input unit.

Abstract: This is a microscope observation system comprising a microscope, a camera unit, a light-amount adjustment unit for controlling adjustment parts included the microscope in order to suppress the amount of reflected light of a specimen image formed on the camera unit, a camera adjustment unit for controlling an adjustment part group of the camera unit in order to adjust image signals photo-electrically converted by the camera unit to a desired state, a light measurement unit for measuring the brightness of the specimen, a display unit for displaying a captured image, a control unit for controlling continuous display speed indicating the continuous display interval of an image continuously displayed on the display unit and at least one of an observation position shifting unit for changing the observation position or observation magnification of the specimen or an observation state detection unit for detecting the observation position or the observation magnification.

Abstract: Disclosed herein is a microscope, including: an illumination optical system; a first image creation optical system; a second image creation optical system; an illumination-field-diaphragm focus adjustment section; and a characteristic-quantity computation block, wherein the illumination-field-diaphragm focus adjustment section adjusts the image creation position for the illumination field diaphragm on the basis of the characteristic quantity computed by the characteristic-quantity computation block.

Abstract: The present invention relates to an autofocus aperture stop (5, 6) in a triangulating autofocusing device (21) for a microscope (40), wherein the autofocus aperture stop (5, 6) comprises at least one diaphragm opening (3, 4) with which a measuring beam pencil (34) used for the autofocusing and running in the direction of the optical axis (18) of the autofocusing device (21) can be limited in its cross section, wherein the diaphragm opening (3, 4) of the autofocus aperture stop (5, 6) is arranged in a decentred position at a spacing from the optical axis (18) of the autofocusing device (21), wherein a decentred autofocus measuring beam (36) can be generated by the diaphragm opening (3, 4) in one half of the cross section (17) of the measuring beam pencil (34).

Abstract: Apparatus for and method of rapid three dimensional scanning and digitizing of an entire microscope sample, or a substantially large portion of a microscope sample, using a tilted sensor synchronized with a positioning stage. The system also provides a method for interpolating tilted image layers into a orthogonal tree dimensional array or into its two dimensional projection as well as a method for composing the volume strips obtained from successive scans of the sample into a single continuous digital image or volume.

Abstract: The present disclosure includes systems and techniques relating to focusing in automated microscope systems. In general, in one implementation, the technique includes obtaining an image of at least a portion of a scan region, analyzing the image to find an area in the image representing a sample, determining a nature of the sample at a selected focus point location in the area in the image, selecting an automated focusing process for use at the selected focus point location based on the determined nature of the sample at the selected focus point location, and focusing the selected automated focusing process. The selecting can include selecting different automated focusing processes for different focus point locations based on different tissue characteristics at the locations.

Abstract: Non-rigidly coupled, overlapping, non-feedback optical systems for spatial filtering of Fourier transform optical patterns and image shape characterization comprises a first optical subsystem that includes a lens for focusing a polarized, coherent beam to a focal point, an image input device that spatially modulates phase positioned between the lens and the focal point, and a spatial filter at the Fourier transform pattern, and a second optical subsystem overlapping the first optical subsystem includes a projection lens and a detector. The second optical subsystem is optically coupled to the first optical subsystem.

Abstract: A microscope with means for adjusting the focal range, comprising a first objective lens for transmitting the object light of an illuminated object in the direction of a detector, with a second objective lens being disposed in the direction of the light upstream of the detector, which second objective lens is followed by a first mirror that can be adjusted in the direction of the optical axis, with at least one second mirror for transmitting light from the first objective lens in the direction of the second objective lens and from the second objective lens to the detector being disposed in the optical path, which second mirror is a fully reflective mirror, or a microscope with means for adjusting the focal range, comprising a first objective lens for transmitting the object light of an illuminated object in the direction of a detector, with a second objective lens being disposed in the direction of light upstream of the detector, which second objective lens is followed by a first mirror that can be adjusted i