Abstract: A microscope may receive a fiber optic connector via a connector adapter of the microscope, wherein the connector adapter includes an opening and a shaped reflective surface surrounding the opening. The microscope may align a ferrule of the fiber optic connector with the opening of the connector adapter of the microscope, wherein the ferrule includes a ferrule endface. The microscope may transmit light onto the shaped reflective surface and may receive reflected light from the ferrule endface and with a camera of the microscope.

Abstract: Microscope imaging and illumination systems and methods are included that may be used to image multiple specimens at different locations relative to a specimen fixture without the need for repositioning a source of illumination. In some cases, light patterns emitted from illumination screens may be repositioned and reconfigured electronically as needed with an illumination signal communicated to such illumination screens. Specialized microscope imaging techniques such as phase contrast microscopy may also be used with the systems and methods discussed herein.

Abstract: A structured illumination microscopic imaging system includes a structured illumination source. A beam shaping lens, an excitation optical filter and a dichroic mirror are provided on the emission light path of the structured illumination source in sequence. An objective lens and a sample are provided on the first optical path of the dichroic mirror in sequence. An emission optical filter, a tube lens, and a detector are provided on the second optical path of the dichroic mirror in sequence. The super-resolution microscopic images with a higher signal-to-noise ratio and higher contrast can be obtained under the premise of lowering the installation and processing precision requirements of the structured illumination microscopic imaging system. Compared to a structured light microscopic imaging system based on digital micromirror arrays or gratings, the system cost is reduced and the system stability is higher.

Abstract: An illumination module (101) for an optical apparatus comprises a light source unit (102), which is configured to selectively emit light along a multiplicity of beam paths (112) in each case. The illumination module (101) also comprises a multiplicity of optical elements (201-203) arranged with lateral offset from one another, wherein each optical element (201-203) of the multiplicity of optical elements (201-203) is configured to transform at least one corresponding beam path (112) of the multiplicity of beam paths.

Abstract: A medical projection apparatus includes: a plurality of projectors each configured to project projection light onto an observation area of an observation optical system, wherein at least two or more projectors of the plurality of projectors are configured to respectively emit projection light to different planes including an optical axis of the observation optical system, and the projection light emitted by the two or more projectors cross each other in any position within a range of at least a possible working distance of the observation optical system.

Abstract: The present invention provides for assessing biological samples for developmental viability utilising microscopy by contemporaneously capturing bright field and dark field images of a biological sample within a time lapse measurement interval.

Abstract: A method for imaging a sample using a microscope having an illumination unit, an imaging lens system and an image sensor, includes: illuminating an area of the sample; imaging and magnifying the sample onto the image sensor and capturing the image using a predetermined number of pixels; providing a plurality of different comparison sample areas; for each comparison sample area, performing a reference measurement, wherein the comparison sample areas are illuminated, imaged and magnified onto the image sensor and captured with the predetermined number of image pixels as a reference image; determining a brightness-correction image with the predetermined number of image pixels by determining the value for each image pixel of the brightness-correction image from the values of allocated image pixels of the reference images, and correcting the image of the area of the sample captured based on the brightness-correction image and outputting it as a corrected image.

Abstract: Various embodiments are directed to a fluorescence reader for assessing a fluorescence intensity of one or more substance samples. The fluorescence reader may comprise a sample support structure for accepting sample wells containing corresponding samples therein, an illumination structure positioned below the sample support structure and configured to illuminate one or more samples positioned within the sample wells, and an imaging structure positioned above the sample support structure and configured to detect a fluorescence emitted by the one or more samples. The illumination structure may comprise one or more reflective perimeter walls, together with the support structure, defining an enclosed illumination cavity, and one or more light sources positioned within the enclosed illumination cavity and configured to emit light causing the one or more samples to fluoresce.

Abstract: The invention relates to a method for tomographic investigation of a sample, in which method a sample is illuminated with an illuminating light bundle and in which a transmitted light bundle that contains the light of the illuminating light bundle transmitted through the sample is detected with a transmission detector. The invention further relates to an apparatus for tomographic investigation of a sample. Provision is made that the illuminating light bundle and the transmitted light bundle pass in opposite propagation directions through the same objective.

Abstract: A system and method for multiple mode inspection of a sample. The system includes a radiation source, an objective lens, a bright field detection module, a dark field detection module and optics. The optics, when the system operates at a first mode, is configured to direct the input beam through a first opening, without substantially blocking any part of the input beam, towards a first region of the objective lens. The optics, when the system operates at a second mode, is configured to direct the input beam through a second opening, without substantially blocking any part of the input beam, towards a second region of the objective lens. The first region of the objective lens differs from the second region of the objective lens.

Abstract: A method for microscopic examination of a specimen includes bringing the specimen into contact with an optically transparent medium that has a higher refractive index than the specimen. An illumination light bundle is generated and directed through an illumination objective that focuses the illumination light bundle. The illumination light bundle that has passed through the illumination objective in the direction of the specimen that is to be examined is deflected using a deflector arranged on a detection objective in such a way that the illumination light bundle strikes a boundary surface between the optically transparent medium and the specimen, where the illumination light bundle is totally reflected for purposes of evanescently illuminating the specimen. Fluorescent light that is emitted by the specimen and that passes through the detection objective is detected.

Abstract: An STED microscope apparatus includes an STED light source for outputting STED light, an excitation light source for outputting excitation light, a phase modulation type SLM for presenting a phase pattern for shaping the STED light in an annular shape by phase modulation, an optical system for irradiating an observation object region with the excitation light and the STED light after phase modulation, a detector for detecting fluorescence generated from the observation object region, and a control unit for controlling the phase pattern. The control unit sets the phase pattern for controlling the inner diameter of the annular shape of the STED light after phase modulation.

Abstract: Disclosed are several technical approaches of using low coherence interferometry techniques to create an autofocus apparatus for optical microscopy. These approaches allow automatic focusing on thin structures that are positioned closely to reflective surfaces and behind refractive material like a cover slip, and automated adjustment of focus position into the sample region without disturbance from reflection off adjacent surfaces. The measurement offset induced by refraction of material that covers the sample is compensated for. Proposed are techniques of an instrument that allows the automatic interchange of imaging objectives in a low coherence interferometry autofocus system, which is of major interest in combination with TDI (time delay integration) imaging, confocal and two-photon fluorescence microscopy.

Abstract: A microscope illumination apparatus includes: a dark-field illumination unit including a light source that is arrangeable in an outer periphery of an observation light path of a microscope, the dark-field illumination unit being removably provided in the outer periphery of the observation light path; a detector that detects that the dark-field illumination unit has been arranged in a prescribed position of the outer periphery of the observation light path, and outputs a turn-on signal; and a controller that controls the light source to be turned on in accordance with the detection of the detector.

Abstract: In accordance with one embodiment of the present invention an apparatus for a low numerical aperture exclusion imaging apparatus is provided. The apparatus may include an electromagnetic illumination source for illuminating a portion of a specimen; and for collecting an image created by the electromagnetic radiation an objective lens optically coupled to the electromagnetic illuminated portion of the specimen. The apparatus also includes an optical blocking plate disposed between the objective lens and a focusing lens. The optical blocking plate is positioned to substantially block undesired electromagnetic radiation from image sources distally aligned in the same optical axis as the specimen. This invention is enhances narrow depth of field characteristics in imaging. It also enhances discreet imaging in a narrow focus field by eliminating some or most of the light which contributes to wide depth of field focus. This is useful for optical sectioning ranging from microscopy to photography.

Abstract: A method for optically measuring height profiles of surfaces, in which an image of the height profile is recorded using an optical recording system, is characterized in that the image is a differential interference contrast image and height gradients within the height profile are represented by intensity gradients, which are quantitatively or qualitatively evaluatable. The surfaces can have structures having a defined profile, in which intensity gradients in the differential interference contrast image, which assume, within a specified tolerance and within a specified range, a value which deviates from a predetermined value or assume a selected value from within a specified tolerance and within a specified range, indicate a defect.

Abstract: A test sample device for an optical microscope which images a sample in different light states with a local resolution in the subwavelength range of the visible spectral range, wherein the test sample device comprises: a test piece, which is designed to be microexamined with the microscope and has a surface on which nanostructures are arranged, wherein each nanostructure, viewed along the surface, has a dimension in the subwavelength range, wherein the nanostructures are spaced apart from one another by an amount which lies above the wavelength of the visible spectral range, and wherein the nanostructures are switchable collectively between a bright state, in which they illuminate, and a dark state, in which they do not illuminate, and a drive, which is designed to move the test piece in the subwavelength range, whereby the different light states can be realized by different movement states of the test piece.

Abstract: A microscope includes a microscope main body and a darkfield objective. The microscope main body includes a light source unit and a main body side fitting unit for fitting an objective, and the darkfield objective includes an optical system for capturing light from a sample surface and a fitting unit that fits in the main body side fitting unit. A darkfield illumination optical path through which light from the light source unit passes is formed in the darkfield objective. When the darkfield objective is attached to the microscope main body, the darkfield objective has incident end of the darkfield illumination optical path outside the fitting unit, where an optical axis of the optical system is central.

Abstract: A method and apparatus are disclosed for generating a plurality of scattered radiation patterns at an image plane of an optical microscope. The apparatus includes at least one lens element, a liquid crystal display (LCD) array, and a housing comprising a body portion supporting the LCD array and lens element in a predetermined spaced apart relationship. The LCD array comprises a plurality of pixel elements arranged in a grid layout, said array being connectable to a processing element adapted to selectively control a transmittance of each pixel in the grid layout.

Abstract: An optical microscope is provided with an adjustable optical phase ring. The adjustable ring provides a way to compensate for distortion in the visible phase ring before the light reaches the sample. In an inverted microscope, when observing transparent cells under a liquid, the visible light phase ring is distorted. By the use of a Liquid Crystal Display (LCD) in place of a fixed ring, the projected ring is adjusted to realign the light and produce phase. In a typical micro plate, the meniscus formed produces a lens effect that is realigned by providing changes in the position and pattern, to allow phase imaging over a wider portion of the well. The realignment of the ring can be manual or automated and can be dynamically adjusted based upon an observed image of the sample.

Abstract: Illuminating arrangement for a microscope (200) having a first LED (10) for providing light with a first intensity spectrum (K1) with at least two intensity maxima and an intensity minimum located between the intensity maxima, and at least one further LED (20) for providing light with a further intensity spectrum (K2) respectively, each further intensity spectrum (K2) having an intensity maximum in the region of the intensity minimum of the first intensity spectrum (K1), and a device (30) for merging the light of the first LED (10) and the light of the at least one further LED (20), by means of which illuminating light can be produced with a combined intensity spectrum (K3) composed of light with the first intensity spectrum and light with each of the further intensity spectra.

Abstract: A microscope includes a light source including an LED device having a light radiating surface and a light directing element including a larger coupling-out surface. The light directing element is disposed so as to couple in light radiated by the light source and couple out the radiated light from the coupling-out surface. The light directing element is disposed so that the light is radiated out in an angular range of ±10° to ±50° and illuminates an area at 5 meters in an angular range of at least ±5° with intensity fluctuations of less than 50%. A condenser is disposed between the coupling-out surface of the light directing element and the object to be viewed. The condenser has an aperture with an aperture dimension and is disposed such that the aperture is irradiated completely with the light coupled out from the coupling-out surface.

Abstract: An observation system includes: a container holding unit; a ring illumination, including a light source in a ring shape, arranged in a position opposed to an outer bottom surface of a container so that a central axis of the illumination is aligned to that of a bottom surface of the container held by the container holding unit, when an observation target is observed; a first light-shielding plate in a ring shape, having an inner diameter capable of varying, arranged between the illumination and the container holding unit, and configured to shield light from the illumination; a lens, arranged in a position opposed to an inner bottom surface of the container held by the container holding unit, to observe the observation target; and a second light-shielding plate, having an outer diameter capable of varying, arranged in an internal space of the illumination, and configured to shield light from the illumination.

Abstract: A light harvesting lens module for collecting an ambient light includes a plurality of annular lenses. The annular lenses are arranged in order from center to outside to form a disc-structure. Each annular lens has a light-input curved surface and a light-output curved surface. The light-input curved surface is in opposition to the center. The light-output curved surface is in opposition to the light-input curved surface, and faces the center. The light is incident on the light-input curved surface, and is refracted and tend to concentrate by the light-input curved surface. Then, the light is emitted from the annular lens, and is refracted to the direction of the center by the light-output curved surface. The ambient light is able to be compressed into a point light by the lens module, so as to increase the concentration ratio and the compression ratio to further get the effective advantage.

Abstract: The disclosed technology relates generally to a method and system for an improved lighting apparatus compatible with imaging small specimens for viewing or photographic purposes. In certain embodiments, the disclosed technology provides an apparatus for projecting light on specimens from opposing light sources at varying distances and angles. In certain embodiments, the apparatus includes an elongated stem that allows the light sources to be repositioned quickly, easily, and accurately. The elongated stem may be flexible and may be used in conjunction with a positioning system, such as a rack and pinion system, thereby allowing precise movement of the light sources.

Abstract: A microscope includes a zoom optical system zooming over a sample, a zoom driving unit moving the optical system along an optical axis, an imaging unit imaging an observation image of the sample through the optical system, thereby generating image data on the sample, and a display unit displaying an image corresponding to the generated image data. A touch panel on a display screen of the display unit accepts an input corresponding to a contact position of an object. A driving control unit outputs a driving signal for changing a zoom magnification of the optical system by setting a middle point between contact positions on the touch panel corresponding to two position signals responsive to an input of the different contact positions as a zoom center position fixed without depending on the zoom magnification of the optical system when the two position signals are output from the touch panel.

Abstract: The invention relates to a ring illumination device (07) for a microscope objective (01), and a microscope objective (01) provided with such a ring illumination device (07). The ring illumination device (07) has at least two LED units (11) which are accommodated in an illumination ring (17) which can be connected to the microscope objective (01). According to the invention, the ring illumination device (07) has at least one lower entrance opening (08) and at least one upper outlet opening (09) for cooling air, for cooling the LED units (11).

Abstract: An observation system includes: a container holding unit; a ring illumination, including a light source in a ring shape, arranged in a position opposed to an outer bottom surface of a container so that a central axis of the illumination is aligned to that of a bottom surface of the container held by the container holding unit, when an observation target is observed; a first light-shielding plate in a ring shape, having an inner diameter capable of varying, arranged between the illumination and the container holding unit, and configured to shield light from the illumination; a lens, arranged in a position opposed to an inner bottom surface of the container held by the container holding unit, to observe the observation target; and a second light-shielding plate, having an outer diameter capable of varying, arranged in an internal space of the illumination, and configured to shield light from the illumination.

Abstract: An ATR objective (1) for an IR microscope has a Cassegrain objective (2), an ATR crystal (7), a holding bar (8) to one end of which on the side of the sample, the ATR crystal (7) is mounted, a holding element (10), thin struts (9) which rigidly connect the holding bar (8) to the holding element (10) and intersect an optical path of the ATR objective (1) entering or exiting the Cassegrain objective (2) in such a fashion that they shade less than 10% of the beam cross-section of the optical path, and a motor drive (12) for axial movement of the holding element (10) relative to the sample position (3). The automated ATR objective thereby enables simple adjustment of operating modes and different contact pressures of the ATR crystal with respect to a sample (19).

Abstract: The invention relates to a method for illuminating an object in a digital light microscope, to a digital light microscope, and to a bright field reflected-light illumination device for a digital light microscope. According to the invention, the bright field reflected-light illumination and the dark field reflected-light illumination are configured with light-emitting diodes as light sources and are individually or jointly drivable via a control unit. Both the bright field reflected-light illumination and the dark field reflected-light illumination are configured as “critical” illumination, in which the light source is imaged into the object plane.

Abstract: A system and method for obtaining super-resolution image of an object. An illumination beam is directed through an optical axis onto the object to be imaged. Paraxial rays of the illumination beam are deflected away from the optical axis and into a beam dump. The non-paraxial rays are collected after being reflected by the object so as to generate an image only from the non-paraxial rays.

Abstract: A light source and/or a deflector and/or the emitting end of an illuminating optical fiber is arranged in the rear focal plane of a lens for total internal reflection microscopy.

Abstract: A method of microscopy and an illumination optical device with a hollow cone for a microscope, the illumination device includes a first conical lens (1) able to receive a collimated incident light beam (10) and form a conical light beam (20), a second conical lens (5) arranged in such a way as to receive the conical light beam (20, 40) and to form a cylindrical light beam with a black background (50) and an optical lens (6) having an image focal plane (12) arranged in such a way as to receive the cylindrical light beam with a black background (50), to form a hollow cone light beam (60) and to focus the hollow cone light beam (60) into a point (18) in the image focal plane (12).

Abstract: A dome illumination for a microscope and a microscope are disclosed. At least one objective lens carries a dome at a free end, wherein the free end of the objective lens is facing a surface of the object. At least one light source is arranged such that an illumination is provided to the dome when the objective lens is positioned in an optical axis or working position of the microscope.

Abstract: A digital microscope system, having one or several objectives, a tube lens system, a digital image recording device, a stand, a holder for a specimen and an illuminating apparatus, and optionally at least one magnification changer. One or several objectives, the tube lens system, the digital image recording device and the illuminating apparatus are integrated into a compact optical assembly, and the optical assembly is joinable to the stand in several versions that differ with regard to the spatial position and orientation of the optical assembly relative to the stand and to the specimen. The spatial position and orientation of the optical assembly relative to the stand and to the specimen may correspond, in a first version of the joining, to an upright microscope configuration and, in a second version of the joining, to an inverted microscope configuration.

Abstract: A microscope subject illumination system including a position targeting accessory that identifies a point on a subject by a distinctive illumination pattern cast on the subject. The system includes an automatic control to switch between illumination sources so that the distinctive pattern is easier to discern during subject positioning. The control may automatically extinguish the targeting illumination after a configurable period of time.

Abstract: A microscope includes a microscope main body and a darkfield objective. The microscope main body includes a light source unit and a main body side fitting unit for fitting an objective, and the darkfield objective includes an optical system for capturing light from a sample surface and a fitting unit that fits in the main body side fitting unit. A darkfield illumination optical path through which light from the light source unit passes is formed in the darkfield objective. When the darkfield objective is attached to the microscope main body, the darkfield objective has incident end of the darkfield illumination optical path outside the fitting unit, where an optical axis of the optical system is central.

Abstract: Optical microscopy of biological specimens, particularly live cells, is difficult as they generally lack sufficient contrast to be studied successfully as typically the internal structures of the cell are colourless and transparent. Commonly, contrast is increased by staining the different structures with selective dyes, but this involves killing and fixing the sample. Staining may also introduce artifacts, apparent structural details caused by the processing of the specimen and are thus not a legitimate feature of the specimen. Further, microscopy of different elements of these biological specimens typically requires multiple microscopy techniques on multiple specimens. According to embodiments of the invention simultaneous imaging techniques are applied to a biological specimen such as fluorescent imaging and dark field imaging by designing an experimental evaluation system and associated illumination system addressing the conflicting demands of these approaches.

Abstract: The present invention relates to an illumination device (400) for a microscope (600), including at least one light source (120, 130) and a reflector (410) for providing diffuse illumination, said reflector at least partially surrounding the observation beam path (OA1) between a microscope objective (10) and an object (20) to be observed. The reflector (410) is at least partially elastic and capable of being reversibly transformed from at least a first form to at least a second form.

Abstract: An objective-type dark-field illumination device for a microfluidic channel is provided and includes an optical stop having a pair of symmetric curved slits used to adjust the optical path and inner numerical aperture of a dark-field light source generated by the device. The dark-field illumination can focus on a smaller spot to illuminate a sample in the microfluidic channel by matching a pin-hole combined with a transmitter objective lens. The optical path and smaller spot is advantageous to solve the problem of a traditional dark-field illumination that may generate background light noise scattered from inner walls of the microfluidic channel to lower the image contrast. Therefore, the signal or image resolution of capturing the scattered light and/or emitted fluorescent light emitted from the sample in the microfluidic channel can be enhanced. Meanwhile, the device can simultaneously excite and detect multiple fluorescent samples with different excited wavelengths in the microfluidic channel.

Abstract: Oblique-illumination systems integrated with fluorescence microscopes and methods of using oblique illumination in fluorescence microscopy are disclosed. An oblique-illumination system is attached to a fluorescence microscope objective. The oblique-illumination system can be used to illuminate from any desired direction the surface of an object located at a fixed known offset away from a sample solution containing fluorescently tagged targets. Oblique illumination is used to illuminate features of the surface while epi-illumination is used to create fluorescent light emitted from the tagged targets. The combination of oblique illumination of the surface and epi-illumination of the targets enables capture of images of the surface features and the fluorescent targets so that the locations of the targets in the sample can be determined based on the locations of the surface features.

Abstract: A microscope system includes: an illumination unit; a light control direction unit specifying quantity of light output from the illumination unit; a storage unit storing voltage-related information in which a direction value specified by the light control direction unit and a voltage value to be applied to the illumination unit are associated with each other and are set, the direction value being in an entire range that can be specified by the light control direction unit under each observation condition, the voltage value corresponding to the direction value; a light quantity control unit controlling the quantity of the emitted light; and a control unit acquiring the voltage value corresponding to the specified direction value from the voltage-related information corresponding to the observation condition, and allowing the light quantity control unit to control the quantity of light based on the acquired voltage value.

Abstract: A measurement apparatus includes a lamp mount including a first mount and a second mount. The first mount has a first cavity to mount an observation module. The second mount has a second cavity to mount an image capture module. The measurement apparatus further includes a plurality of light modules mounted on an undersurface of the lamp mount. The second mount is disposed with an included angle relative to a first axis of the first cavity so that a second axis of the second cavity and the first axis converge on a point. The undersurface of the lamp mount is concave so that light from the light modules tilts toward the first axis, and the light and the first axis also converge on the point.

Abstract: A microscope system and an illumination intensity adjusting method that can automatically adjust an illumination intensity when a bright-field illumination system and a dark-field illumination system are simultaneously used are provided. The microscope system includes: a stage on which a specimen is placed; an objective lens that converges observation light from at least the specimen S on the stage; a bright-field illumination unit configured to emit bright-field illumination light that is illumination light aperture to the specimen for a bright-field observation; a dark-field illumination unit configured to emit dark-field illumination light that is illumination light aperture to the specimen for a dark-field observation; and an illumination intensity control unit configured to adjust an illumination intensity of at least one of the bright-field illumination light and the dark-field illumination light according to the illumination intensity of the other one.

Abstract: A particle image-capturing method includes controlling a light-emitting diode unit to emit light so as to illuminate a fluid through an annular condenser, particles in which scattering the light, and controlling an image-capturing unit to capture images of the scattered light through a microscope. A micro particle image velocimetry that performs the method is also disclosed.

Abstract: The automatic adjustment method of a microscopic image for automatically adjusting an image on the basis of the lightness of the microscopic image includes distinguishing an observation pixel being an observation target in the image from a non-observation pixel not being an observation target on the basis of the lightness of each pixel of the image, determining a representative value for representing the lightness of the image on the basis of the lightness of a selection pixel identified as the observation pixel and adjusting the lightness of the image on the basis of the representative value.

Abstract: A microscope, which moves an objective lens along an observation optical axis with respect to a specimen, includes an imaging unit and a supporting unit. The imaging unit has an imaging lens, which is arranged on the observation optical axis and forms an observation image of the specimen, and an imaging element, which is arranged on the observation optical axis and takes the observation image, and is optically connected to the objective lens by a parallel light flux. The supporting unit fixedly supports the imaging unit, and movably supports the objective lens.

Abstract: An optical microscope is provided with an adjustable optical phase ring. The adjustable ring provides a way to compensate for distortion in the visible phase ring before the light reaches the sample. In an inverted microscope, when observing transparent cells under a liquid, the visible light phase ring is distorted. By the use of a Liquid Crystal Display (LCD) in place of a fixed ring, the projected ring is adjusted to realign the light and produce phase. In a typical micro plate, the meniscus formed produces a lens effect that is realigned by providing changes in the position and pattern, to allow phase imaging over a wider portion of the well. The realignment of the ring can be manual or automated and can be dynamically adjusted based upon an observed image of the sample.

Abstract: The present invention relates to a fluorescent microscope and a remote control system thereof. The present invention reduces the size of the fluorescent microscope to facilitate transportation and management and be disposed in a narrow place such as the inside of the incubator or the clean bench, etc. and observes the samples through a remote control, thereby making it possible to improve the user convenience.

Abstract: A multi-mode optical fiber delivers light from a radiation source to a multi-focal confocal microscope with reasonable efficiency. A core diameter of the multi-mode fiber is selected such that an etendue of light emitted from the fiber is not substantially greater than a total etendue of light passing through a plurality of pinholes in a pinhole array of the multi-focal confocal microscope. The core diameter may be selected taking into account a specific optical geometry of the multi-focal confocal microscope, including pinhole diameter and focal lengths of relevant optical elements. For coherent radiation sources, phase randomization may be included. A multi-mode fiber enables the use of a variety of radiation sources and wavelengths in a multi-focal confocal microscope, since the coupling of the radiation source to the multi-mode fiber is less sensitive to mechanical and temperature influences than coupling the radiation source to a single mode fiber.