Abstract: The present invention relates to a device and a method for qualitative and quantitative analysis of heavy metals and more particularly provides a device and a method for qualitative and quantitative analysis of heavy metals utilizing a rotary disc system.

Abstract: Among other thing, the present invention provides solution to the problem, particularly, the present invention certain surfaces and certain sample holder to improve the sensitivity, speed, and easy-to-use of an optical signal based assays, such as colorimetric assays or fluorescence assays.

Abstract: The invention provides a mild procedure for the functionalization of cellulose and other substrates with a detection reagent such as N-(1-naphthyl)ethylenediamine and is able to achieve much higher functionalization density than previously reported. A paper-based device created using cellulose functionalized according to the invention allowed for much lower detection limits for nitrite in various kinds of water samples than have been seen using paper-based devices. In addition, grafting of N-(1-naphthyl)ethylenediamine to cellulose improved the stability of the N-(1-naphthyl)ethylenediamine in the presence of moisture and light.

Abstract: Disclosed herein are methods of producing a test element for studying a body fluid sample. In the methods, a detection layer is covered with a polymeric spreading layer and applied to a support. The spreading layer can be produced by being sprayed onto the detection layer such that the spreading layer has a thickness of at most about 20 ?m. Also disclosed are test elements produced by the methods, as well as tape cassettes incorporating such test elements.

Abstract: Integrated diagnostic devices comprising peptide-DNA conjugates for analyte detection, an embedded biomolecular computing system for sample analysis, and a layered device architecture are provided herein. In particular, provided herein are devices comprising a layered architecture that enables diagnostic reagents, sample components, and reaction products to flow through the system with minimal user intervention.

Abstract: Coating compositions are described that include one or more rare metal components, such as rare alkali metal components, as well as diagnostics test elements that incorporate the same. Methods also are described for determining an amount of a dried coating composition in a coat based upon the rare metal components.

Abstract: The present invention relates to extracts, compounds isolated from Cakile arabica for use in the treatment of ulcer and to pharmaceutical compositions thereof.

Abstract: A canister containing an active agent wherein the canister includes a canister body having a peripheral body wall defining an upper opening and a non-removable snap-on cap. The cap includes a peripheral skirt. The peripheral body wall of the canister includes a step formed between a first portion of the peripheral body wall and a second portion located beneath the first portion. The first portion is thinner than the second portion to form the step, wherein an inner shape of the peripheral skirt and an outer shape of the first portion of the peripheral body wall form a snap-on fit, wherein the cap surrounds the first portion. A vertical extension of the peripheral skirt is smaller than a vertical extension of the first portion of the peripheral body wall to form a gap between the step and the peripheral skirt.

Abstract: Apparatus for performing an assay to detect the presence of an analyte in a test sample. A housing defines a slot for receiving a sample collector, and a capsule contains a buffer liquid, the capsule being sealed by an openable lid, and being connected to the housing such that insertion of a sample collector into the slot causes the lid to open releasing the buffer liquid into the slot. The housing further defines an incubation chamber containing or configured to receive a reagent, and an aperture permitting liquid communication between the slot and the incubation chamber. The apparatus comprises one or more test elements, a substantially liquid tight sealing member separating the incubation chamber and the test element(s), and an activation mechanism operable to open the liquid tight sealing member thereby bringing at least a portion of the or each test element into liquid communication with the incubation chamber.

Abstract: Disclosed is an ultrahigh-sensitivity two-dimensional chromatography-based biosensor, wherein cross-flow of high driving force across a narrow membrane is applied, thereby enabling the elimination of background noise, efficient supply of a large-sized polymeric or high-molecular-weight signal tracer, sequential material supply for increased reactivity, and simultaneous washing and signal generation when additional signal generation is required, as in an enzyme.

Abstract: A method of quantitatively determining the concentration of at least one analyte in a sample by: (i) adding a portion of the sample to a first analyte assay formulation and to an analyte assay reference formulation to generate a first analyte sample and analyte reference sample and determining the concentration of the at least one analyte in the sample and/or (ii) adding a portion of the sample to a second analyte assay formulation and determining the concentration of the at least one analyte in the sample, as well as formulations, kits of parts, systems and computer implemented methods associated with the method.

Abstract: Lateral flow devices, methods and kits for performing lateral flow western blot assays are provided.

Abstract: Systems and methods are described for isolation, separation and detection of a molecular species using a low resource device for processing of samples. Methods include isolation, separation and detection of whole cells.

Abstract: A device for immunochromatographic assay includes a housing and a vessel body. The housing has an upwardly-extending hollow protrusion for housing the vessel body. The vessel body has a vessel body wall and a vessel body bottom. A raised tip and a fence surrounding the tip are arranged in the housing. The vessel body and the raised tip can be moved towards each other such that the raised tip can break the vessel body bottom to release liquid in the vessel body.

Abstract: The present invention intends to provide an immunochromatographic test piece that makes it possible to achieve both highly sensitive detection of a substance to be detected and a simple test piece structure, which are usually difficult to be made compatible with each other.

Abstract: The method provides methods and compositions for treating metabolic disorders such as impaired glucose tolerance, elevated blood glucose, insulin resistance, dyslipidemia, obesity, and fatty liver.

Abstract: The present invention relates to a process for the detection and adsorption of arsenic from ground water and industrial waste water using lanthanide doped nanoparticles. More particularly, the present invention provides a process for the detection and adsorption arsenic in ppm level using Eu0.05Y0.95PO4 nanoparticles.

Abstract: The present invention provides a method for manufacturing a microbial detection device, microbial detection method, microbial detection kit and microbial detection device. The manufacturing method includes the following steps: defining a sampling portion and a reaction portion on a substrate. Fiber materials are disposed in the reaction portion and a surface of the reaction portion which contacts with the fiber materials comprises abundant hydroxyl groups. Reaction reagents are then added into the fiber materials. An acidic solution is applied to treat the fiber materials and the hydroxyl groups in the reaction portion.

Abstract: The technology described in this document can be embodied in a test strip for use in measuring a level of an ST2 cardiac biomarker in a blood plasma sample. The test strip includes a base, and a plurality of conjugates, wherein each conjugate includes a reporter group bound to a first antibody that binds to ST2. A conjugate pad disposed along a length of the base and is configured to hold the plurality of conjugates that bind with ST2 to produce conjugate-ST2 complexes. The conjugate pad is further configured to receive the blood plasma sample. The test strip also includes a plurality of second and third antibodies that bind to ST2, and the conjugate-ST2 complexes, respectively. The plurality of second antibodies are bound to a membrane in a test location and the plurality of third antibodies are bound to the membrane in a control location.

Abstract: The invention provides methods and devices for isolating chromatin from liquid samples. The devices have a rigid porous matrix on which a ligand is immobilized, with the ligand binding to a protein associated with the chromatin.

Abstract: Disclosed is a cell wall C-polysaccharide antigen of Streptococcus pneumoniae which contains not more than 10% by weight of protein, and preferably less which has been purified with 0.1N Na OH prior to deproteinizing. Also disclosed are polyvalent antibodies raised against Streptococcus pneumoniae which have been affinity purified by passing them over a chromatographic affinity matrix to which is coupled the purified and at least partially deproteinized antigen to render them antigen-specific.

Abstract: Devices and methods incorporate mucolytic agents into a point-of-care testing device. The sample is loaded, and then the sample travels until it encounters one or more lysis agents and/or mucolytic agents. The mucolytic agent is preferably pre-loaded onto the collection device. In a preferred embodiment, the mucolytic agent is localized between the sample application zone and the conjugate zone. In embodiments with a sample compressor, one or more mucolytic agents may be pre-loaded and dried on the sample compressor, the sample collector, in various locations on the test strip, or in the running buffer.

Abstract: Systems include test cells with sorbent material in a T-shape and defining a first flow path for a solution and a second flow path for a sample, and a test line or test site with immobilized antigens or antibodies or other ligand binding molecules located at the junction of the T. A housing houses the sorbent material and defines a first hole adjacent an end of the first flow path for receiving the solution and a second hole adjacent an end of the second flow path for receiving the sample.

Abstract: Provided are methods, processes and systems for treating a soapstock. In alternative embodiments, provided are systems and methods for treating a soapstock to generate free fatty acids and/or fatty acid derivatives, e.g. fatty acid alkyl esters. In alternative embodiments, provided are systems and methods for realizing the full fatty acid yield of a soapstock by first converting substantially all of the saponifiable material in a soapstock to salts of fatty acids (soaps) and acidulating the soaps to generate free fatty acids and/or fatty acid derivatives, e.g. fatty acid alkyl esters, wherein the soapstock comprises soaps and saponifiable lipids, e.g. glycerides and/or phospholipids, and the generating of free fatty acids and/or fatty acid is achieved.

Abstract: A rapid diagnostic test device uses driven flow technology to significantly expedite the testing time of a sample. The rapid diagnostic test device can be used to analyze liquids, such as some body fluids, by using labeled molecular affinity binding, such as immunochromatography. The test device can detect an analyte, such as an antibody or antigen, which may indicate a particular condition, the presence of a particular drug, or the like. The device includes a cover and base that combine to form an internal cavity for receiving a test strip. Compression bars in the cover and a compression cushion in the base sandwich a conjugate pad of the test strip to accelerate chemical mixtures to flow from the conjugate pad of the test strip toward fixed sites along the strip from which readings are taken.

Abstract: Test elements are disclosed for detecting at least one analyte concentration in a sample. The test elements are used for detecting one or more analytes such as metabolites in body fluids, especially glucose. The test elements correct for an interfering variable such as temperature and or hematocrit in a test element system via a correction factor based upon diffusion of at least one diffusable label.

Abstract: A test carrier includes a base member and a cover member. The base member includes a multi-layer board including a wiring line that is electrically connected to a die and a base film that supports the multi-layer board. The cover member includes a frame-shaped cover frame having an opening formed therein. The size of the multi-layer board is larger than the size of the die and is smaller than the size of the opening in a direction along a surface that is opposite to the die.

Abstract: A rapid diagnostic test device uses driven flow technology to significantly expedite the testing time of a sample. The rapid diagnostic test device can be used to analyze liquids, such as some body fluids, by using labeled molecular affinity binding, such as immunochromatography. The test device can detect an analyte, such as an antibody or antigen, which may indicate a particular condition, the presence of a particular drug, or the like. The device includes a top and bottom that combine to form a fluid channel through which a fluid is forced from a fluid collector. A press pad in the device may press against a press pad on a test strip to accelerate chemical mixtures to flow from the conjugate color pad of the test strip toward fixed sites along the strip from which readings are taken.

Abstract: The following description relates to a composite for a harmful material-detecting sensor, which includes a dye having a color-change function by reaction with acid or base and a polymer material, a method for producing the composite for the harmful material-detecting sensor, and a low-cost film-type color change harmful material sensor, which includes the composite for the harmful material-detecting sensor so as to quickly detect a harmful material and can be carried by an individual.

Abstract: A non-invasive gas analyte sensing and monitoring system and method is provided that is particularly applicable to transcutaneous monitoring of arterial blood gases in a mammal. The system and method relies on diffusion of the analyte to be measured into a diffusion chamber and remote sensing of the analyte in the diffusion chamber using optical chemical sensors and associated optoelectronics.

Abstract: The system includes a simple system for CD4 and CD8 counting in point-of-care HIV staging within resource poor countries. Unlike previous approaches, no sample preparation is required with the sample added directly to a chip containing dried reagents by capillary flow. A large area image cytometer consisting of an LED module is used to excite the fluorochromes PerCP and APC labeled targets and a monochrome CCD camera with a combination of two macro lenses captures images of 40 mm2 of blood (approximately 1 micro liter). CD4 and CD8-T-lymphocyte counts correlate well with those obtained by flow cytometry. The cytometer system described in the present invention provides an affordable and easy-to-use technique for use in remote locations.

Abstract: A cartridge and method for conducting a labeled molecular affinity binding test such as antibody/antigen, ligand/receptor, and colorometric reactions, and other chemical reactions for which one or more analytes is present in a liquid sample formate. A progressive compression structure progressively forces a liquid flow out of a conjugate pad toward a reaction region to more thoroughly and rapidly mix the liquid, encouraging specific first affinity binding to the analytes in question. A specially dimensioned constricting passageway surrounding the reaction region including the result zones provides an additional siphoning force to the flow. These combined forces rapidly and more evenly guide the flow of liquid through the reaction region so that the rushed rate of uptake of analytes at the strip lines are more evenly distributed, adhesive attachment of non-specific molecules is largely avoided, vastly improving sensitivity and specificity, and providing quantitative results in some tests.

Abstract: Apparatuses and methods for removing carbon dioxide and other pollutants including heavy metals from a gas stream are provided. The methods include obtaining a water-condensate from the flue-gas stream; obtaining a chloride salt; admixing the salt with water, steam, or both to produce a solution; electrolyzing the solution to produce a hydroxide and chlorine gas; admixing a portion of the hydroxide with the flue-gas stream to produce carbonate products, bicarbonate products, or a mixture of carbonate and bicarbonate products in an admixture; separating said carbonate and/or bicarbonate products from the admixture, thereby removing carbon dioxide from the flue-gas stream; adding a portion of the hydroxide to the water-condensate to change the pH of the water-condensate from acidic to basic, resulting in precipitation of the heavy metals; and passing the water-condensate through a filtering medium.

Abstract: A temperature measuring insert composed of ceramic or sintered materials of magnesium oxide or aluminum oxide accomodates, on its end facing the measured medium, temperature sensor elements, such as thermocouples and temperature measuring resistors, as a measuring tip, installable singly or with others in metal or ceramic protective tubes. The site of the measuring tip, a water adsorption behavior differing from the remaining measuring insert material is present, in order to protect the temperature sensor elements in the case of rapid process related temperature changes, in order to prevent, in the case of heating, effects due to superheated steam.

Abstract: A method may involve forming one or more photoresist layers over a sensor located on a structure, such that the sensor is covered by the one or more photoresist layers. The sensor is configured to detect an analyte. The method may involve forming a first polymer layer. Further, the method may involve positioning the structure on the first polymer layer. Still further, the method may involve forming a second polymer layer over the first polymer layer and the structure, such that the structure is fully enclosed by the first polymer layer, the second polymer layer, and the one or more photoresist layers. The method may also involve removing the one or more photoresist layers to form a channel through the second polymer layer, wherein the sensor is configured to receive the analyte via the channel.

Abstract: The invention provides a modified form of saturation assay, which is based on the measurement of a free or unbound labelled reagent fraction (such that there is an increase in signal in the presence of analyte) and employs trapping zones to concentrate said unbound or free labelled fraction to avoid loss of sensitivity. Preferably, the assay is a membrane assay.

Abstract: A problem to be solved by the present invention is to provide an immunochromatographic test strip and a detection method using immunochromatography avoiding aggregation of colloidal gold conjugates while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a blood-agglutinating agent and the colloidal gold conjugates as a detection reagent. To solve the problem, the present inventers reviewed a past reagent configuration itself from a completely different viewpoint rather than selecting type and amount of polyanions and, as a result of extensive study on each element, the inventers surprisingly found that aggregation of colloidal gold can be suppressed by using a certain buffer solution without using neutralization by polyanions.

Abstract: A method for sampling a sulphur-containing solid product including supplying a gas flow comprising hydrogen sulphide, bringing the gas flow into contact with a solid reagent and reacting the solid reagent with the hydrogen sulphide contained in the gas flow, the reaction fixing the sulphur of the hydrogen sulphide by forming a sulphur-containing solid product which is different in color from the solid reagent, and recovering the sulphur-containing solid product. The invention also relates to a device suitable for the implementation of this method.

Abstract: A method for generating and localizing a signal in a solid phase substrate detection system comprises applying a solution of target material to a substrate; binding the target with a specific affinity molecule having an attached label, the label comprising multiple signal precursor molecules; applying a carrier to the substrate, and treating the label to convert the signal precursor molecules to signal generating molecules. The carrier comprises solvent for the label and thickener for localizing the signal. The carrier may include developer that converts signal precursor molecules to signal generating molecules. Developer is not necessary if the signal precursor molecules are converted to signal generating molecules by e.g. temperature change, pH change, sonication, light irradiation, microwave heating. A test device for detecting target in a fluid sample, and a kit of parts for determining the presence of target in a fluid sample are also disclosed.

Abstract: A problem to be solved by the present invention is to provide an immunochromatographic test strip and a detection method using immunochromatography avoiding aggregation of colloidal gold conjugates while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a blood-agglutinating agent and the colloidal gold conjugates as a detection reagent. To solve the problem, the present inventers reviewed a past reagent configuration itself from a completely different viewpoint rather than selecting type and amount of polyanions and, as a result of extensive study on each element, the inventers surprisingly found that aggregation of colloidal gold can be suppressed by using a certain buffer solution without using neutralization by polyanions.

Abstract: An improved apparatus and method for dispersion of a labeling conjugate in a diagnostic assay, the result being a one-step assay. By eliminating a conjugate pad as in conventional lateral diagnostic devices, and forming a frazil ice pellicle (FIP), rehydration and flow are improved resulting in better reproducibility, improved sensitivity, and reduced costs of individual assay devices. The formation of a frazil ice film formed on a super cooled surface of a sample receiving means simplifies assay assembly. Lyophilization of the FIP improves the release of a sample/analyte/label matrix into a macro channel as in a direct flow assay, while at the same time allowing reagents to mix and flow, thereby optimizing the assay performance. The reagents of the conjugate and the formation of the FIP stabilize the conjugate proteins and provide extended shelf life to the diagnostic assay device.

Abstract: The invention is directed towards methods and compositions for identifying the amount of hydrofluoric acid in a buffered oxide etching composition. In buffered oxide etching compositions it is very difficult to measure the amount of hydrofluoric acid because it has varying equilibriums and it is toxic so it hard to handle and sample. When used to manufacture microchips however, incorrect amounts of hydrofluoric acid will ruin those chips. The invention utilizes a unique method of spectrographically measuring the hydrofluoric acid when in contact with added chromogenic agents to obtain exact measurements that are accurate, immediate, and safe.

Abstract: An optic light guide test sensor comprises a light guide, a reagent-coated membrane, and a mesh layer. The reagent-coated membrane and the mesh layer are attached to the light guide at an output end of the light guide. The light guide test sensor is adapted to be used to test the level of an analyte in a biological fluid sample when used with a readhead. A method of manufacturing the light guide test sensor involves providing a plurality of light guides, providing a strip of reagent-coated membrane, and providing a strip of mesh layer. The reagent-coated membrane and mesh layer are attached to the light guides by ultrasonic welding. The reagent-coated membrane and mesh layer may also be attached to the light guides by adhesive.

Abstract: Systems and methods for detecting the presence of biomolecules in a sample using biosensors that incorporate resonators which have functionalized surfaces for reacting with target biomolecules. In one embodiment, a device includes a piezoelectric resonator having a functionalized surface configured to react with target molecules, thereby changing the mass and/or charge of the resonator which consequently changes the frequency response of the resonator. The resonator's frequency response after exposure to a sample is compared to a reference, such as the frequency response before exposure to the sample, a stored baseline frequency response or a control resonator's frequency response.

Abstract: This invention provides a method of promoting bone healing by locally administering a vanadium-based insulin mimetic agent to a patient in need thereof. The invention also provides a new use of insulin-mimetic vanadium compounds for manufacture of medicaments for accelerating bone-healing processes. In addition, the invention also encompasses a bone injury treatment kit suitable for localized administration of insulin-mimetic vanadium compounds or compositions thereof to a patient in need of such treatment.

Abstract: A portable tester to detect one or more analytes from explosives, drugs, or household chemicals, comprising: a plurality of chemical disposable, detachable reagent reservoirs storing reagents to induce a series of color reactions, wherein developed color depends on sequences of applied reagents to respective pads on a support base, reaction times and temperatures on said pad to create one or more color bar codes for a specific analyte. The tester comprises a piezoelectric dispenser coupled to each respective reservoir to dispense a mist of reagent onto respective test pads to increase sensitivity and reduce reaction heat and time; wherein piezoelectric dispenser is processor controlled to control dispensing time and volume in each step.

Abstract: The invention is directed towards methods and compositions for identifying the amount of ammonium acid in a buffered oxide etching composition. In buffered oxide etching compositions it is very difficult to measure the amount of ammonium acid because it has varying equilibriums and it is toxic so it hard to handle and sample. When used to manufacture microchips however, incorrect amounts of ammonium acid will ruin those chips. The invention utilizes a unique method of spectrographically measuring the ammonium acid when in contact with added chromogenic agents to obtain exact measurements that are accurate, immediate, and safe.

Abstract: A device and a process are proposed for separating constituents of a sample fluid, wherein the sample fluid is supplied by capillary force to a receiving region (7) for metering, stopping or delaying the sample fluid and the sample fluid is pre-treated with a soluble chemical (13) in the receiving region (7) before the sample fluid is supplied to a separating device (5) for separating off constituents of the sample fluid.

Abstract: A wetness indicator material may be used on a substrate to form a wetness sensor. The sensor may show either the presence or absence of an aqueous-based fluid or water-containing medium, such as vaginal fluid or urine in a personal hygiene article. The wetness sensor may be incorporated into the article. The wetness indicator material includes a standard colorant that does not change color when wetted. The standard colorant increases the range of hues exhibited by the wetness indicator material.

Abstract: The present invention provides a biomedical device comprising a porous hydrophilic substrate; a hydrophobic material; a fluid inlet; two or more test zones, wherein fluid vertically flows through the porous hydrophilic substrate and then distributes into the test zones. The present invention further provides a method for making a biomedical device.