Abstract: This invention provides devices, systems, and methods for performing point-of-care, analysis, including multiplexed analysis, of a biological fluid analyte, such as blood. The invention includes a cartridge for collecting the biological fluid analyte. The cartridge is configured to be inserted into an assay reader, in which one or more assay reactions may be performed. The assay reader is designed to read and report the results of the one or more assay reactions.

Abstract: A miniature blower includes a soft sheet, a nozzle plate, a chamber frame, an actuator body, an insulation frame, and a conductive frame. The nozzle plate has a suspension portion, and the soft sheet is disposed on the suspension portion. The chamber frame is disposed on the nozzle plate. The actuator body includes a piezoelectric carrier plate, an adjusting resonance plate, and a piezoelectric plate. The actuator body is disposed on the chamber frame. The insulation frame is disposed on the actuator. The center point of a central hole of the soft sheet and the center point of a hollow hole of the suspension portion are located at the same axis.

Abstract: A method for manufacturing a microfluidic device can include providing a base component to define a first portion of the microfluidic device. A cap component of the microfluidic device can be fabricated with a sealing lip extending a first distance from a first side of the cap component and a support portion extending a second distance, less than the first distance, from the first side of the cap component. The method can include positioning the cap component and the base component within a mold to bring the sealing lip of the cap component in contact with the base component. The base component, the support portion of the cap component, and the sealing lip of the cap component together can define a cavity. The method can include injecting a polymer material into the mold to cause the polymer material to fill the cavity.

Abstract: A packaging for the stocking and transportation of glass bottles including a box closed on five sides and open at the top, configured to house a plurality of bottles therein, arranged side-by-side in a vertical position, and a lid couplable in a removable manner with the box, in a position facing a bottom wall of the box; wherein the lid is provided, on a face thereof configured to be facing, in use, the bottom wall, with a plurality of spacer elements, which protrude in a cantilever fashion from the face of the lid and towards the bottom wall of the box; the spacer elements being configured to be interposed, in use, between each bottle contained in the box and respective other bottles immediately adjacent thereto and/or between some bottles and a side wall of the box.

Abstract: A fluidic device for culturing cells includes a microplate and plate lid. The microplate includes multiple wells and channels, the channels extending between the wells such that the channels interconnect the wells. The plate lid releasably engages the microplate to thereby enclose the wells and the channels. The wells include a culture surface such that a cell culture medium received therein is deposited over the culture surface. At least one channel that extends between adjacent ones of the wells is spaced from the culture surfaces of the adjacent wells defining a gap between the at least one channel and the culture surfaces of the adjacent wells for collection of the cell culture medium.

Abstract: Microfluidic network device (2) configured to supply reagents to a biological tissue sampling device (1), comprising a plurality of microfluidic inlet channels (12) connected to respective sources of said reagents, at least one common outlet channel (22), and a plurality of valves (36) interconnecting an outlet end (14) of each of said plurality of inlet channels to said at least one common outlet channel.

Abstract: A transfection device suitable for delivery of various macrostructures (e.g., mitochondria, bacteria, liposomes) is described and uses mechanical force to thereby induce active endocytosis in a target cell. Contemplated devices are able to achieve high throughput of transfected cells that remain viable and are capable of producing colonies.

Abstract: It is provided a measuring cartridge (1) for measuring at least one constituent of a liquid sample, in particular blood, and for performing quality control, the cartridge comprising: a casing (3) insertable into a reception opening (51) of a measuring instrument (50), the casing (3) at least partly surrounding an inner space (5); wherein the inner space contains: a measurement cell (7) comprising a reception space (9) for the sample and at least one sensor area (11) with which the sample is in contact when loaded into the reception space (9); plural quality control containers (13a,13b,13c) for respectively holding different quality control solutions (15a,15b,15c); a solution routing system (17) adapted to selectively route one of the quality control solutions (15a,15b,15c) from the respective quality control container (13a,13b,13c) into the reception space (9) of the measurement cell (7).

Abstract: A microfluidic chip includes a flow passage plate, a flat plate, and an annular seal. In the flow passage plate, a recess forming a flow passage for liquid and a communication hole communicating with the recess are formed. The flat plate is stacked on or under the flow passage plate to close the recess for defining the flow passage. In the flat plate, a communication through-hole communicating with the recess is formed. The annular seal is located on, or formed on, an outer surface of at least one of the flow passage plate and the flat plate, the annular seal surrounding at least one of the communication hole and the communication through-hole. The annular seal is made of an elastomer.

Abstract: A reaction processing vessel includes a substrate and a groove-like channel formed on the upper surface of the substrate. The channel includes a high temperature serpiginous channel, a medium temperature serpiginous channel, and a high temperature braking channel and a medium temperature braking channel that are adjacent to the high temperature serpiginous channel and the medium temperature serpiginous channel, respectively. The respective cross-sectional areas of the high temperature braking channel and the medium temperature braking channel are larger than the respective cross-sectional areas of the high temperature serpiginous channel and the medium temperature serpiginous channel, respectively.

Abstract: Biochemical test methods for protein quantification can include application of foam-suppressing and/or foam-destroying substances to avoid readout problems and ensure the accuracy of measurement results. One such method for determining the amount of one or more proteins in a solution includes providing at least a first solution that is known to contain or suspected of containing one or more protein(s), adding at least one foam-suppressing and/or foam-destroying substance to the first solution, resulting in a second solution, and determining the amount of the one or more protein(s) in the second solution.

Abstract: Multi-well collapsible basket arrays and methods for their use with high throughput culture and histology analysis of spheroids and organoids. Such a collapsible basket array includes a collapsible cellular array structure having multiple cells and connectors that interconnect adjacent pairs of the cells to cause the collapsible cellular array structure to collapse from an expanded configuration to a collapsed configuration in which the connectors are partially wrapped around perimeters or circumferences of the cells, whereby the collapsible cellular array structure is expandable to acquire an expanded configuration capable of individually aligning the cells thereof with wells of a well plate. The collapsible basket array further includes inserts individually mountable to the cells of the collapsible cellular array structure, with each insert including a permeable basket with pores sized to retain spheroids or organoids within the basket.

Abstract: A sample analysis substrate includes a substrate; a first holding chamber; a reaction chamber; a first flow path having a first opening and a second opening respectively connected with the first holding chamber and reaction chamber; a main chamber; a second flow path having a third opening and a fourth opening respectively connected with the reaction chamber and the main chamber; and a magnet accommodation chamber capable of accommodating a magnet. The first opening is located closer to a rotation shaft than the second opening. The second opening is located closer to the rotation shaft than the third opening. The magnet accommodation chamber is located at a position at which, in the case where the magnet is accommodated in the magnet accommodation chamber, the magnet captures magnetic particles in the main chamber. The sample analysis substrate is rotatable to transfer a liquid.

Abstract: Object interference in biological samples generated by lateral shearing interference microscopes is addressed by a shearing microscope slide comprising a periodic structure having alternating reference and sample regions. In some embodiments, the reference regions are configured to provide references that remove sample overlap in a sheared microscopic measurement. A system for generating sheared microscopic measurements is also provided that comprises an inlet configured to receive a sample material, an outlet configured to release a portion of the sample material, and a periodic structure having a plurality of interleaved reference and sample channels. In some cases, the sample channels are configured to accommodate a flow of sample material from the inlet to the outlet and the reference channels are configured to provide references that remove sample overlap in a sheared microscopic measurement.

Abstract: Techniques for making fluid flow devices are described. The technique is based on radiation-induced conversion of a radiation-sensitive substance from a first state to a second state. With adjustment of the radiation parameters such as power and scan speed we can control the depths of barriers that are formed within a substrate which can produce 3D flow paths. We have used this depth-variable patterning protocol for stacking and sealing of multilayer substrates, for assembly of backing layers for two-dimensional (2D) lateral flow devices and for fabrication of 3D devices. Since the 3D flow paths can be formed via a single laser-writing process by controlling the patterning parameters, this is a distinct improvement over other methods that require multiple complicated and repetitive assembly procedures.

Abstract: Analytical cartridges, systems and methods of processing a sample for analysis using capillary flows. Vertical gradient sample filtration provides filtrate to an incubation chamber for a time controlled by a flow modulator at the outlet of the incubation chamber. The flow modulator can include a serpentine capillary flow path without side walls. Incubated filtrate can flow from the incubation chamber to a detection channel after a predetermined time. The detection chamber can include one or more analytical regions in a porous substrate for detection of two or more analytes on the same cartridge from the same sample.

Abstract: The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.

Abstract: A cartridge includes a plate including a fluid inlet and a fluid outlet, a biochip disposed under the plate, and a first adhesive layer bonding the plate and the biochip. A fluid channel is formed between the plate and the biochip. The fluid inlet and the fluid outlet are in fluid communication with the fluid channel.

Abstract: The purpose of the present invention is to provide a flow reactor that can ensure safety even if leakage occurs at the connections of the lines. The flow reactor of the present invention is characterized by including one or more line structures, each of the line structures including a raw material feeding line, a reactor unit to react a raw material fed from the raw material feeding line, and a discharge line to discharge a reaction product produced in the reactor unit, wherein the flow reactor includes a vessel in which part or all of the reactor unit and a fluid are accommodated to be capable of being in contact with each other, wherein each of the line structures includes two or more attachable and detachable connections, and wherein at least one of the attachable and detachable connections is accommodated in the vessel.

Abstract: Described are embodiments of an invention for detecting target antigens in a biological sample using a sample assembly. Detection may be accomplished by performing a method comprising: sweeping a head module over the sample assembly, wherein said head module includes at least one magneto-resistive read sensor configured to detect target antigens via nanoparticles within the sample assembly; and detecting at least one particular antigen among the target antigens. Preferably, detecting the target antigens via the nanoparticles is based at least in part on detecting unique magnetic properties of particular nanoparticles specifically associated with different types of the target antigens. Detection using a magnetic read/write head in the sample assembly facilitates automation of sample detection with high speed and fidelity. Corresponding systems are also disclosed.

Abstract: This invention provides devices, systems, and methods for performing point-of-care, analysis, including multiplexed analysis, of a biological fluid analyte, such as blood. The invention includes a cartridge for collecting the biological fluid analyte. The cartridge is configured to be inserted into an assay reader, in which one or more assay reactions may be performed. The assay reader is designed to read and report the results of the one or more assay reactions.

Abstract: This invention provides devices, systems, and methods for performing point-of-care, analysis, including multiplexed analysis, of a biological fluid analyte, such as blood. The invention includes a cartridge for collecting the biological fluid analyte. The cartridge is configured to be inserted into an assay reader, in which one or more assay reactions may be performed. The assay reader is designed to read and report the results of the one or more assay reactions.

Abstract: This invention provides devices, systems, and methods for performing point-of-care, analysis, including multiplexed analysis, of a biological fluid analyte, such as blood. The invention includes a cartridge for collecting the biological fluid analyte. The cartridge is configured to be inserted into an assay reader, in which one or more assay reactions may be performed. The assay reader is designed to read and report the results of the one or more assay reactions.

Abstract: Arrays and substrates comprising a material, in particular capture agents and/or detectable targets, attached to the substrates along substantially parallel lines forming a barcoded pattern and related methods and systems.

Abstract: A method for forming a microchannel or microfluidic reservoir is described. Methods provided herein include applying a covering over a recess formed in a substrate and bonding the covering to the substrate along seams. Methods provided herein use a substrate having elevated edge regions bordering the recess that project from the surface of the substrate. Bonding seams nm a distance from the elevated edge regions of the recess such that the covering and the elevated edge regions are pressed against each other. Devices for bonding substrates and coverings to make microchannels and microfluidic reservoirs are also provided herein. Devices provided herein can include pressing elements for holding the substrate and the covering together. Pressing elements provided herein can include recesses and/or elastic layers positioned opposite to one or more recesses in a substrate.

Abstract: A microfluidic multi-well-based cell culture testing device is provided. The multi-well-based cell culture testing device has an array structure of a plurality of aligned microfluidic well units. Each of the microfluidic well units comprises an inlet through which a first fluid enters, an accommodation compartment adapted to accommodate a second fluid therein, a microfluidic channel through which the first fluid flows, and an air outlet adapted to facilitate the entering of the first fluid.

Abstract: Methods, devices, and systems for performing isoelectric focusing reactions are described. The systems or devices disclosed herein may comprise fixtures that have a membrane. In some instances, the disclosed devices may be designed to perform isoelectric focusing or other separation reactions followed by further characterization of the separated analytes using mass spectrometry. The disclosed methods, devices, and systems provide for fast, accurate separation and characterization of protein analyte mixtures or other biological molecules by isoelectric point.

Abstract: A miniature pneumatic device includes a miniature fluid control device and a miniature valve device. The miniature fluid control device includes a gas inlet plate, a resonance plate, a piezoelectric actuator and a gas collecting plate. The length and width of the gas collecting plate are between 4 mm and 10 mm. A first chamber is formed between the resonance plate and the piezoelectric actuator. After a gas is fed into the gas inlet plate, the gas is transferred to the first chamber through the resonance plate and then transferred downwardly. Consequently, a pressure gradient is generated to continuously push the gas. The miniature valve device includes a valve film and a gas outlet plate. After the gas is transferred from the miniature fluid control device to the miniature valve device, the valve opening of the valve film is correspondingly opened or closed and the gas is transferred in one direction.

Abstract: A method for handling, in a microfluidic system, microdrops which include samples, including the steps of forming, in an oil, microdrops of an aqueous solution containing a sample, the oil and/or the aqueous solution containing a sample including a gelling agent; trapping the microdrops by means of surface-tension traps pre-arranged in a trapping area; and at least partially gelling the oil in the trapping area and/or at least partially gelling the trapped microdrops.

Abstract: The present invention provides a method for the purification of 227Th from a mixture comprising 227Th and 223Ra, said method comprising: i) preparing a first solution comprising a mixture of 227Th and 223Ra ions dissolved in an aqueous solution of first mineral acid; ii) loading said first solution onto a strong base anion exchange resin; iii) eluting 223Ra from said strong base anion exchange resin using a second mineral acid in an aqueous solution; iv) optionally rinsing said strong base anion exchange resin using a first aqueous medium; v) eluting 227Th from said strong base anion exchange resin using a third mineral acid in an aqueous solution whereby to generate a second solution comprising 227Th. The invention further provides a purified 227Th solution, a corresponding pharmaceutical formulation and methods of treatment of neoplastic disease.

Abstract: A microfluidic device includes a substrate, a microchannel, a plurality of spaced-apart pillars, and a porous nanofiber structure. The substrate has a substrate top surface. The microchannel is indented downwardly from the substrate top surface. The pillars are disposed in the microchannel. Each of the pillars has a pillar top surface that is lower in level than the substrate top surface. The porous nanofiber web structure is formed in the microchannel, and includes a first web portion residing in a space formed among the pillars.

Abstract: A multi-well plate lid includes a well-side surface to cover a plurality of wells of a multi-well plate. The well-side surface includes two or more regions with different adhesive strengths to the multi-well plate.

Abstract: A cartridge has a container with at least one well, protrusions distributed on the container base side, and a flat polymer film having a lower surface and a hydrophobic upper surface kept at a distance (d) to the container base side by the protrusions. The container and the film are reversibly attachable to a liquid droplet manipulation instrument so that the lower surface of the film abuts at least one electrode array of the instrument. The container enables displacement of at least one liquid droplet from a well onto the hydrophobic upper surface of the flat polymer film and above the electrode array. The liquid droplet manipulation instrument has a control unit with a voltage control and an electrode selector for individually selecting each electrode of the electrode array and for providing the selected electrode with a voltage.

Abstract: A micro-fluidic chip comprises a chip base, a lens, and a securing portion. The chip base has a flow cell and a micro-fluidic channel defined therein. The micro-fluidic channel is fluidly connected to the flow cell to deliver fluid to and from the flow cell, respectively via a fluid input port and a fluid output port. The lens has an apex and a base. The apex is positioned within the flow cell. The securing portion is affixed to the chip base such that the lens is sandwiched between the chip base and the securing portion. The securing portion has a circular cavity defined therein in a surface adjacent the chip base, for receiving the base of the lens. The securing portion further has separate light input and output channels to allow light in and out, respectively, of the circular cavity and the lens.

Abstract: A miniature fluid control device includes a piezoelectric actuator and a housing. The piezoelectric actuator comprises a suspension plate, an outer frame, at least one bracket and a piezoelectric ceramic plate. The piezoelectric ceramic plate is attached on a first surface of the suspension plate and has a length not larger than that of the suspension plate. The housing includes a gas collecting plate and a base. The gas collecting plate is a frame body with a sidewall and comprises a plurality of perforations. The base seals a bottom of the piezoelectric actuator and has a central aperture corresponding to the middle portion of the suspension plate. When the voltage is applied to the piezoelectric actuator, the suspension plate is permitted to undergo the curvy vibration, the fluid is transferred from the central aperture of the base to the gas-collecting chamber, and exited from the perforations.

Abstract: A specimen processing cartridge includes a reservoir having a fluid inlet, an elastic diaphragm, and a fluid outlet. The reservoir is operable to receive a volume of liquid from the fluid inlet, and the fluid outlet is positioned along a fluid flow path between the reservoir and a downstream reservoir. The cartridge includes a dissolvable membrane that occludes flow through the fluid outlet when the dissolvable membrane is in a first, undissolved state, and that permits flow from the reservoir to the downstream reservoir when in a second, dissolved state. The elastic diaphragm is operable to pressurize the reservoir upon receiving the volume of liquid when the dissolvable membrane is in the first, undissolved state, and is operable to contract and propel at least a portion of the volume of liquid from the reservoir to the downstream reservoir when the dissolvable membrane is in the second, dissolved state.

Abstract: Microscale fluidic devices and components thereof having a fluid retention groove, as well as systems and methods related thereto. The fluid retention groove facilitates uniform bonding of microfluidic device components.

Abstract: A process for depositing an inorganic material on a substrate, the process comprising, providing a substrate having a surface, providing a precursor mixture comprising a metal sulfonate, and delivering the precursor mixture to the surface of the substrate, wherein the surface of the substrate is at a substrate temperature of above 450° C. and is sufficient to effect decomposition of the metal sulfonate. The inorganic material may include a metal or a metal oxide. The preferred metal sulfonate is metal triflate.

Abstract: A miniature pneumatic device includes a miniature fluid control device and a miniature valve device. The miniature fluid control device includes a gas inlet plate, a resonance plate, a piezoelectric actuator and a gas collecting plate. A first chamber is formed between the resonance plate and the piezoelectric actuator. After a gas is fed into the gas inlet plate, the gas is transferred to the first chamber through the resonance plate and then transferred downwardly. Consequently, a pressure gradient is generated to continuously push the gas. The miniature valve device includes a valve plate and a gas outlet plate. After the gas is transferred from the miniature fluid control device to the miniature valve device, the valve opening of the valve plate is correspondingly opened or closed and the gas is transferred in one direction. Consequently, a pressure-collecting operation or a pressure-releasing operation is selectively performed.

Abstract: Methods and systems are provided to facilitate simultaneous high-resolution microscopic imaging of cells and detection of side-scattered light from such cells using an immersion objective. A container maintains a volume of an immersion oil or other immersion fluid in contact with the immersion objective and with a stage that contains a sample of the cells. The container also includes a window through which the cells can be illuminated off-axis to generate side-scattered light. The side-scattered light can then be detected through the immersion objective. The container maintains the immersion fluid in contact with an internal surface of the window to control the geometry of the optical interface between the off-axis illumination source and the immersion fluid. These systems permit high-throughput identification and imaging of cells for biological research, improvement of side-scatter cell classifiers, improved high-throughput cell sorting, and other applications.

Abstract: A substrate surface defect detection device includes an optical waveguide for receiving first light and directing the received first light to a surface of a to be tested substrate, the optical waveguide having a first surface facing toward the substrate and a second surface facing away from the substrate, a microlens array disposed on the second surface of the optical waveguide, the microlens array including a plurality of microlenses arranged in an array for receiving second light from the surface of the to be tested substrate and converging the received second light to converged light, and an imaging component for receiving the converged light from the at least one microlens array for optical imaging. The substrate surface defect detection device requires significantly less time than conventional substrate surface defect detection devices.

Abstract: The present disclosure is directed to a nitrocellulose-based propellant composition comprising: (a) a nitrate ester based propellant comprising nitrocellulose; and (b) a stabilizer consisting of a tocopherol compound with a general formula (I), wherein: X is oxygen; R1 is selected from the group consisting of hydrogen, alkyl, alkenyl, alkynyl, aryl, heteroaryl, carboxylic acid, carboxylate, ester, saccharide, alkoxy-linked saccharide, alcohol, and ethers; R2 is selected from the group consisting of hydrogen methyl, benzyl carboxylic acid, benzyl carboxylate, benzylester and saccharide; R3 is selected from the group consisting of hydrogen, methyl, benzyl carboxylic acid, benzyl carboxylate, benzylester and saccharide; R4 is selected from the group consisting of methyl, benzyl carboxylic acid, benzyl carboxylate, benzylester and saccharide; and R5 is selected from the group consisting of alkyl and alkenyl.

Abstract: A miniature fluid control device includes a gas inlet plate, a resonance plate and a piezoelectric actuator. The gas inlet plate includes at least one inlet, at least one convergence channel and a central cavity. A convergence chamber is defined by the central cavity. The resonance plate has a central aperture. The piezoelectric actuator includes a suspension plate, an outer frame and a piezoelectric ceramic plate. A gap is formed between the resonance plate and the piezoelectric actuator to define a first chamber. When the piezoelectric actuator is driven and after the gas is fed into the miniature fluid control device through the inlet of the gas inlet plate, the gas is sequentially converged to the central cavity through the convergence channel, transferred through the central aperture of the resonance plate, introduced into the first chamber, transferred downwardly through the piezoelectric actuator, and exited from the miniature fluid control device.

Abstract: The present disclosure is directed to an auto-sampling system with syringe, valve configurations, and control logic that allow automatic, inline preparation of concentrated sulfuric acid and concentrated phosphoric acid for analytic analyzes. In implementations, the auto-sampling system includes independent syringe pumps connected to a valve system to dynamically introduce carrier, diluent, buffer, and eluent flows according to one or more modes of operation.

Abstract: A miniature pneumatic device includes a miniature fluid control device and a miniature valve device. The miniature fluid control device includes a gas inlet plate, a resonance plate, a piezoelectric actuator and a gas collecting plate. A first chamber is formed between the resonance plate and the piezoelectric actuator. After a gas is fed into the gas inlet plate, the gas is transferred to the first chamber through the resonance plate and then transferred downwardly. Consequently, a pressure gradient is generated to continuously push the gas. The miniature valve device includes a valve plate and a gas outlet plate. After the gas is transferred from the miniature fluid control device to the miniature valve device, the valve opening of the valve plate is correspondingly opened or closed and the gas is transferred in one direction. Consequently, a pressure-collecting operation or a pressure-releasing operation is selectively performed.

Abstract: An apparatus includes a device for storing a liquid sample, in which the device has a sample acceptance well, one or more storage chambers, and one or more fluidic channels fluidly coupling the sample acceptance well to the one or more storage chambers. The apparatus also includes a well plate having a plate and multiple wells formed in the plate, in which the device and the well plate are configured to be attached to one another.

Abstract: This invention provides devices, systems, and methods for performing point-of-care, analysis, including multiplexed analysis, of a biological fluid analyte, such as blood. The invention includes a cartridge for collecting the biological fluid analyte. The cartridge is configured to be inserted into an assay reader, in which one or more assay reactions may be performed. The assay reader is designed to read and report the results of the one or more assay reactions.

Abstract: A liquid handling device having an upstream liquid handling structure connected to a downstream liquid handling structure by a conduit. The upstream and downstream liquid handling structures are sealed or sealable such that the conduit provides the only fluidic communication paths between the upstream and downstream liquid handling structure. The device is arranged such that a driving force causes liquid in the upstream liquid handling structure to at least partially fill the conduit to separate gas in the upstream liquid handling structure from gas in the downstream liquid handling structure. With the device set up such that the two gas volumes are separated by a liquid volume, gas pressures and liquid flow in the device can be controlled by control of the driving force. The device may be arranged for rotation about an axis of rotation to provide the driving force.

Abstract: A microfluidic device and method comprises a rotatable substrate configured to be rotated at various angular velocities in order to accomplish a series of media exchanges with a sample chamber for containing a sample. A plurality of media reservoirs and waste reservoirs are connected to the sample chamber via channels forming capillary valves in which the capillary valves have varying burst frequencies of rotation of the substrate. The substrate can then be rotated through a series of angular velocities to automate media exchanges between the sample chamber and the respective media reservoirs and waste reservoirs. A pair of media reservoirs are located radially inward of the sample chamber and a pair of waste reservoirs are located radially outward of the sample chamber in an “X” configuration such that centrifugal forces cause fluid media to open the capillary valves and flow among the sample chamber, media reservoirs and waste reservoirs.

Abstract: A reversible bonded microfluidic structure comprises an overhanging cap or gasket structure atop a continuous microfluidic channel wall. An overhanging gasket structure may reduce stress concentrations at the edge of the channel wall and can permit improved reversible adhesion of the channel wall and adjacent dry adhesive fibers. In one example, reversible adhesion of the overhanging channel wall gasket and adjacent dry adhesive fibers may approach 1 MPa in axial loading. An overhanging gasket structure of the microfluidic channel wall may comprise a single “fiber” that is continuous around the perimeter of the desired microfluidic channel shape, and may define a self-sealing gasket which will contain fluid. The overhanging gasket structure may be surrounded by further overhanging or undercut dry adhesive fibers to enhance the adhesion and help make the rest of the surface more tolerant to defects and surface roughness.