Multiple Stages Of Fermentation; Multiple Types Of Micro-organisms Or Reuse Of Micro-organisms Patents (Class 435/162)
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Publication number: 20140075603Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 14, 2013Publication date: March 13, 2014Inventors: Lan Tang, Ye Liu, Junxin Duan, Wenping Wu, Randall Kramer
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Publication number: 20140075601Abstract: The present invention relates to alpha-amylases, nucleic acids encoding the alpha-amylases, methods of producing the alpha-amylases, and methods of using the alpha-amylases.Type: ApplicationFiled: October 28, 2013Publication date: March 13, 2014Applicant: NOVOZYMES A/SInventors: Carsten Andersen, Thomas Agersten Poulsen
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Patent number: 8669083Abstract: An integrated system produces ethanol and biogas from raw plant materials. The system includes a pretreatment apparatus for converting raw plant materials into sugars and a fermenter for fermenting the sugars to produce a beer including ethanol. A distillation apparatus separates the beer into the ethanol and a whole stillage, and a separator then separates the whole stillage into a thin stillage and wet distillers grains. A biogas apparatus processes a first portion of the thin stillage to produce biogas and a biogas effluent, and converts a percentage of the non-fermentable solids and organic acids in the thin stillage into biogas. The pretreatment apparatus is supplied with an amount of fresh water and an amount of backset, the backset including the biogas effluent recycled from the biogas apparatus to the pretreatment apparatus.Type: GrantFiled: June 15, 2009Date of Patent: March 11, 2014Assignee: Eisenmann CorporationInventors: Eberhard Veit, Ondrej Stonawski, Adam Halsband
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Publication number: 20140065685Abstract: A system and process for producing ethanol and biogas. An incoming feedstock and water is directed to a preparation unit that frees sugar from the feedstock. The feedstock is directed to a fermenter that ferments the sugar to produce a beer that includes ethanol and feedstock. The beer is directed to a distillation unit which separates the ethanol from the feedstock and produces ethanol and whole stillage. The stillage derived is direct to an anaerobic membrane bioreactor unit. The stillage is subjected to anaerobic digestion in the anaerobic digester and this produces mixed liquor that includes suspended solids. Mixed liquor including the suspended solids is directed to the membrane separation unit which filters the suspended solids and produces a concentrated reject (retentate) stream and a backset permeate stream. The backset permeate stream is then mixed with the incoming feedstock.Type: ApplicationFiled: August 29, 2012Publication date: March 6, 2014Inventors: Graig Rosenberger, Timur Dunaev
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Publication number: 20140065671Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity, catalytic domains, and cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains, and cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, or cellulose binding domains.Type: ApplicationFiled: January 26, 2012Publication date: March 6, 2014Applicants: Novozymes, Inc., Novozymes A/SInventors: Mary Ann Stringer, Brett McBrayer
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Publication number: 20140065677Abstract: The present invention relates to isolated polypeptides having cellulolytic activity or hemicellulolytic activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: January 26, 2012Publication date: March 6, 2014Applicant: Novozymes A/SInventors: Lene Lange, Peter K. Busk
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Publication number: 20140065683Abstract: A process for the production of alcohols and/or solvents starting from cellulosic or lignocellulosic biomass comprising at least: a) Thermochemical pretreatment of a cellulosic or lignocellulosic substrate; b) Optionally, washing pretreated substrate and setting the pH; c) Enzymatic hydrolysis of pretreated, optionally washed, substrate, using cellulolytic and/or hemicellulolytic enzymes producing a hydrolyzate and a water-insoluble residue; d) Ethylic fermentation of hexoses in hydrolyzate obtained from c) into ethanol by alcohologenic microorganism and production of ethyl wine; e) One extraction stage comprising e1) Separation and purification of ethanol and/or solvents obtained from d), and e2) Separation of a solid cake containing insoluble residue and production of vinasses; f) Butylic fermentation of pentoses contained in vinasses obtained in e2) by a solventogenic microorganism and production of butyl wine; at least a portion of butyl wine is recycled upstream from at least one enzymatic hydrolysType: ApplicationFiled: April 4, 2012Publication date: March 6, 2014Applicant: IFP ENERGIES NOUVELLESInventors: Marcel Ropars, Caroline Aymard, Rejane Dastillung, Remy Marchal, Sandra Menir, Benjamin Clement
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Publication number: 20140051141Abstract: A process for increasing alcohol yield from grain that includes adding cellulase enzymes or a mixture of cellulase enzymes to break down cellulostic feedstock, which is typically discarded. The cellulase enzymes or a mixture of cellulase enzymes may be added to a conventional alcohol production process either through a joint or separate fermentation process.Type: ApplicationFiled: August 12, 2013Publication date: February 20, 2014Applicant: Arisdyne Systems, Inc.Inventors: Oleg KOZYUK, Peter REIMERS
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Publication number: 20140051130Abstract: The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.Type: ApplicationFiled: October 11, 2013Publication date: February 20, 2014Inventors: Brett McBrayer, Tarana Shaghasi, Elena Vlasenko
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Publication number: 20140051142Abstract: The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.Type: ApplicationFiled: October 28, 2013Publication date: February 20, 2014Applicant: Novozymes A/SInventors: Lene Lange, Wenping Wu, Dominique Aubert, Sara Landvik, Kirk Matthew Schnorr, Ib Groth Clausen
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Publication number: 20140051143Abstract: The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.Type: ApplicationFiled: October 28, 2013Publication date: February 20, 2014Applicant: NOVOZYMES A/SInventors: Lene Lange, Wenping Wu, Dominique Aubert, Sara Landvik, Kirk Matthew Schnorr, Ib Groth Clausen
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Patent number: 8652819Abstract: Provided herein are methods for producing a fermentation product, such as ethanol, by co-culture of a member of the genus Paenibacillus and an ethanologenic microbe, such as yeast or E. coli. Also provided are methods for making enzymes useful in the saccharification of a pretreated lignocellulosic material. The enzymes may be made by culturing a member of the genus Paenibacillus in a composition suitable for production of such enzymes. An example of such a composition is a pretreated lignocellulosic material, for example, spent hydrolysates. Also provided are genetically modified members of the genus Paenibacillus that have been genetically modified to not produce an antimicrobial, for instance, a polymyxin E.Type: GrantFiled: May 22, 2009Date of Patent: February 18, 2014Assignee: University of Georgia Research Foundation, Inc.Inventors: Joy Doran Peterson, Emily DeCrescenzo-Henriksen
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Patent number: 8652818Abstract: A method of producing bioproducts from a feedstock in a system configured to produce ethanol and distillers grains from a fermentation product is disclosed. A system configured to process feedstock into a fermentation product and bioproducts including ethanol and meal is disclosed. A bioproduct produced from a fermentation product produced from a feedstock in a biorefining system is disclosed.Type: GrantFiled: December 31, 2009Date of Patent: February 18, 2014Assignee: POET Research, Inc.Inventors: John Warren Lawton, Jr., Jason Alan Bootsma, Stephen Michael Lewis
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Publication number: 20140045227Abstract: The present invention provides a process for degradation of lignocellulosic biomass, which has optionally been pre-treated. It is based on the finding that hydrolysis efficiency of the biomass is enhanced in the presence of a mechanically or chemically treated microorganism capable of producing the respective hydrolytic enzymes. The invention therefore provides a process for degradation of lignocellulosic biomass with integrated produced enzyme cocktails. The invention also provides a process wherein the part of the optionally pre-treated lignocellulosic biomass is incorporated into the final growth medium of the fungus.Type: ApplicationFiled: January 2, 2012Publication date: February 13, 2014Applicant: CLARIANT PRODUKTE (DEUTSCHLAND) GMBHInventors: Markus Rarbach, Zdravko Dragovic, Andreas Kohl, Jochen Gerlach, Jörg Bartuch, Thomas Brück
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Publication number: 20140045236Abstract: The present invention relates to separation of desired target products from biological, plant, and waste-type material, wherein the desired target products include renewable fuels such as ethanol, biobutanol, and biodiesel, wherein the separation is conducted with a cross-flow filtration system having the ability to separate desired products from both non-viscous and viscous medium.Type: ApplicationFiled: February 21, 2012Publication date: February 13, 2014Applicant: SMARTFLOW TECHNOLOGIES, INC.Inventors: Todd Benson, Kim Davis, Jason Bell, James Kacmar
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Publication number: 20140045226Abstract: A process for treating lignocellulosic biomass is described. The process includes treating particulate biomass with alkali to partially remove hemicellulose sheath on cellulose microfibrils of the biomass; and carrying out an oxidation reaction on the alkali treated biomass to form a treated biomass having a disrupted cellulose crystalline structure. The treated biomass may be further processed to form free sugars and lignin and the free sugars can be used to produce bio-ethanol or used as sugar source in fermentation processes.Type: ApplicationFiled: July 9, 2013Publication date: February 13, 2014Inventors: J. Bruce WICKING, Yilin BIAN, Ping LIU, Monlin Kuo
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Patent number: 8647851Abstract: A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing in a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity of greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.Type: GrantFiled: December 8, 2011Date of Patent: February 11, 2014Assignee: Ineos Bio LimitedInventors: James L. Gaddy, Dinesh K. Arora, Ching-Whan Ko, John Randall Phillips, Rahul Basu, Carl V. Wikstrom, Edgar C. Clausen
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Publication number: 20140038848Abstract: The present invention provides for a method of genetically modifying microorganisms to enhance resistance to ionic liquids, host cells genetically modified in accordance with the methods, and methods of using the host cells in a reaction comprising biomass that has been pretreated with ionic liquids.Type: ApplicationFiled: July 3, 2013Publication date: February 6, 2014Applicant: Lawrence Livermore National Security, LLCInventors: Thomas Lawrence Ruegg, Michael P. Thelen
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Publication number: 20140038252Abstract: The present invention relates to a process for the production of ethanol and butanol from biomass, and in particular to a process for the production of ethanol and butanol using two separate fermentation step subjecting the biomass feedstock to anaerobic fermentation at a pH below 6.0 and at a temperature in the range 20 to 700° C. and so as to convert the biomass to a product predominantly comprising acetic acid and butyric acid with at least a 2:1 ratio by weight of acetic acid to butyric acid, c) treating the product of stream of step (b) to separate a solution comprising the acetic acid and butyric acid by: (i) separating a solution comprising the acetic acid and butyric acid from any residual solids and (ii) separating bacteria and/or pasteurizing or sterilizing the solution from the first fermentation step, and d) in a second fermentation step fermenting the solution comprising the acetic acid and butyric acid from the step (c) to form ethanol and butanol.Type: ApplicationFiled: October 10, 2013Publication date: February 6, 2014Applicant: INEOS BIO SAInventors: Peter Simpson Bell, Stephen John Benstead, Neil Turnbull
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Patent number: 8642301Abstract: A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing in a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity of greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.Type: GrantFiled: December 7, 2011Date of Patent: February 4, 2014Assignee: Ineos Bio LimitedInventors: James L. Gaddy, Dinesh K. Arora, Ching-Whan Ko, John Randall Phillips, Rahul Basu, Carl V. Wilkstrom, Edgar C. Clausen
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Patent number: 8642302Abstract: A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing in a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity of greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.Type: GrantFiled: December 12, 2011Date of Patent: February 4, 2014Assignee: Ineos Bio LimitedInventors: James L. Gaddy, Dinesh K. Arora, Ching-Whan Ko, John Randall Phillips, Rahul Basu, Carl V. Wilkstrom, Edgar C. Clausen
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Publication number: 20140030767Abstract: The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca2+ to the parent alpha-amylase.Type: ApplicationFiled: May 31, 2013Publication date: January 30, 2014Applicant: Novozymes A/SInventor: Novozymes A/S
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Publication number: 20140030769Abstract: Disclosed herein are combinations of free fungal enzymes and cellulosomes useful for the hydrolysis of cellulose and the conversion of biomass. Methods of degrading cellulose and biomass using the combinations are also disclosed.Type: ApplicationFiled: July 29, 2013Publication date: January 30, 2014Applicant: Alliance for Sustainable Energy, LLCInventors: Michael RESCH, John O. BAKER, Xu QI, William S. ADNEY, Steven R. DECKER, Michael E. HIMMEL, Bryon DONOHOE
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Publication number: 20140030763Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation.Type: ApplicationFiled: June 20, 2013Publication date: January 30, 2014Inventor: Marshall Medoff
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Publication number: 20140024096Abstract: A method for enhancing the treatment of lignocellulose-containing materials by biotreatment wherein such lignocellulose-containing materials, normally resistant to biotreatment, are first subjected to a low-temperature, long-residence time pyrolysis at about 175° C. to about 325° C. for about 0.1 hour to about 2.0 hours, wherein a substantial portion of the incoming material is distilled into water-soluble compounds amenable to anaerobic biotreatment. Exemplary applications of the method include pyrolytic pre-treatment of wastewater sludges, cellulosic wastes, wood, peat, plant residues, low-grade coal, and the like to enhance methane gas production in anaerobic digestion and/or oxygen-limited or oxygen-starved fermentation to produce ethanol.Type: ApplicationFiled: September 19, 2013Publication date: January 23, 2014Applicant: F. Michael Lewis, Inc.Inventor: F. Michael LEWIS
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Publication number: 20140017737Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: September 23, 2013Publication date: January 16, 2014Inventor: Mark Wogulis
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Publication number: 20140017732Abstract: The present application describes a dilute two-loop method for treating biomass in the production of biofuels such as ethanol. The method involves washing the biomass to dissolve sugars that are associated with the biomass, and separating the washed biomass into a solids phase and a liquids phase. The solids phase is pretreated to render the biomass more susceptible to hydrolysis under conditions that do not produce substantial amounts of sugars. The pretreated biomass is separated into a second solids phase and second liquid phase, and the second solids phase is saccharified and fermented. The first and second liquid phases are recycled to dilute the biomass at various stages of the process.Type: ApplicationFiled: March 15, 2013Publication date: January 16, 2014Applicant: Edeniq, Inc.Inventor: Edeniq, Inc.
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Publication number: 20140011250Abstract: The present invention provides a fermentation process for producing a fermentation product from starch-containing material wherein one or more antibacterial agents are added before and/or during fermentation.Type: ApplicationFiled: November 4, 2011Publication date: January 9, 2014Applicant: NOVOZYMES A/SInventors: Randy Deinhammer, Rikke Monica Festersen
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Publication number: 20140011241Abstract: The present invention enables isomerization process flows at lower pH, lower temperature, and in the presence of certain inhibiting compounds by using the xylose isomerase enzyme from the microorganism Fulvimarina pelagi. The xylose isomerase from this marine bacterium not only is very active at the fermentation pH and temperature, it is also tolerant of xylitol and calcium in the amounts generated in biomass fermentation conditions. For the HFCS application, the xylose isomerase from Fulvimarina pelagi is pH compatible with the process and is tolerant of calcium in the amounts found in the HFCS process. The temperature optimum is similar to commercially available amylase/gluco-amylase products.Type: ApplicationFiled: December 21, 2012Publication date: January 9, 2014Inventors: Christopher Charles Beatty, Stephen Jairus Potochnik, Joshua Brandon Kitner
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Publication number: 20140004588Abstract: The present invention provides enriched polynucleotides, and enriched polypeptides having pectinase activity. The present invention also includes methods of using the polynucleotides and polypeptides described herein.Type: ApplicationFiled: July 15, 2013Publication date: January 2, 2014Applicant: University of Georgia Research Foundation, Inc.Inventor: JOY DORAN PETERSON
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Publication number: 20140004587Abstract: The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.Type: ApplicationFiled: June 17, 2013Publication date: January 2, 2014Inventors: Nigel DUNN-COLEMAN, Frits GOEDEGEBUUR, Michael WARD, Jian YAO
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Publication number: 20140007301Abstract: The present invention relates to isolated polypeptides having glucoamylase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 30, 2010Publication date: January 2, 2014Applicants: NOVOZYMES A/S, NOVOZYMES NORTH AMERICA, INCInventors: Marc D. Morant, Mikako Sasa, Keiichi Ayabe, Guillermo Coward-Kelly
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Publication number: 20140004570Abstract: Provided herein are methods of increasing the efficiency of biomass saccharification. In particular, the methods include ways of avoiding feedback inhibition during the production of useful products.Type: ApplicationFiled: September 3, 2013Publication date: January 2, 2014Applicant: XYLECO, INC.Inventors: Marshall MEDOFF, Thomas Craig MASTERMAN, Michael W. FINN
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Publication number: 20130330782Abstract: Described are processes that include the non-enzymatic, hydrolytic liquefaction of lignocellulosic biomass to form digest slurries and heat recovery from such digest slurries. Due to enhanced flow properties of the digest slurries such heat recovery can be efficiently conducted in spiral, plate and frame or other heat exchanger designs, with the recovered heat going to unit operations of the process such as heating incoming pretreatment media for the liquefaction. Processes can also involve additional hydrolytic digestion of some or all of the initial slurry components with enzyme and/or additional heat recovery from the initial slurry by direct contact heat exchange in which a portion of the digest slurry liquids is flashed to vapor and that vapor is condensed onto incoming lignocellulosic biomass to the process. Processes as described can be integrated into ethanol manufacture by fermentation of sugars from the digested compositions.Type: ApplicationFiled: July 29, 2011Publication date: December 12, 2013Applicant: Purdue Research FoundationInventor: Purdue Research Foundation
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Publication number: 20130330791Abstract: The present disclosure relates to an improved process of producing a fermentation product, in particular ethanol. The present disclosure relates also to the use of enzymes for improving the quality of by-products in the fermentative production process and to compositions comprising enzymes capable of degrading components in the fermented mash in the fermentation process.Type: ApplicationFiled: December 21, 2011Publication date: December 12, 2013Applicant: DIREVO INDUSTRIAL BIOTECHNOLOGY GMBHInventors: Klaudija Milos, Steffen Köhler, Christian Elend, Léonie Degener
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Patent number: 8597934Abstract: Ethanol and other liquid products are produced from biomass using gasification of the biomass to produce a syngas containing CO2, CO, H2 and sulfur or sulfur compounds that passes the syngas to a fermentation step for the conversion of the CO and CO2 and H2 to ethanol. Sulfur and sulfur compounds in the syngas are used to satisfy sulfur demanded by bacteria in the fermentation step. A sulfur control additive is added to the gasification to control syngas sulfur and sulfur compounds at a desired concentration to meet bacteria sulfur demand.Type: GrantFiled: October 28, 2010Date of Patent: December 3, 2013Assignee: Coskata, Inc.Inventor: Richard E. Tobey
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Patent number: 8597914Abstract: Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention.Type: GrantFiled: January 9, 2013Date of Patent: December 3, 2013Assignee: Proterro, Inc.Inventors: John Aikens, Robert J. Turner
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Publication number: 20130316407Abstract: The invention relates to a process of producing a fermentation product in the presence of pyridoxamine.Type: ApplicationFiled: February 6, 2012Publication date: November 28, 2013Applicant: NOVOZYMES A/SInventor: Joyce Craig
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Publication number: 20130309729Abstract: The invention relates to Mutant cellobiohydrolase, being a mutant of SEQ ID NO:1, having a substitution at position N247(I,F,H,W) of SEQ ID NO: 1, wherein the mutant cellobiohydrolase has at least 50% sequence identity with SEQ ID NO: 1, and wherein the mutant cellobiohydrolase has CBHI activity.Type: ApplicationFiled: January 30, 2012Publication date: November 21, 2013Applicant: DSM IP ASSETS B.V.Inventors: Jan Metske Van Der Laan, Margot Elisabeth Francoise Schooneveld-Bergmans, Denise Ilse Jacobs
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Patent number: 8574879Abstract: A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing in a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity of greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1.Type: GrantFiled: December 14, 2011Date of Patent: November 5, 2013Assignee: Ineos Bio LimitedInventors: James L. Gaddy, Dinesh K. Arora, Ching-Whan Ko, John Randall Phillips, Rahul Basu, Carl V. Wilkstrom, Edgar C. Clausen
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Publication number: 20130288296Abstract: The present invention relates to methods of degrading or converting a cellulosic material pretreated with a composition comprising one or more GH61 polypeptides.Type: ApplicationFiled: November 2, 2011Publication date: October 31, 2013Applicant: Novozymes, Inc.Inventors: Jason Quinlan, Feng Xu
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Publication number: 20130288300Abstract: Provided are isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: February 23, 2012Publication date: October 31, 2013Applicant: Novoozymes A/SInventors: Yu Zhang, Junxin Duan, Lan Tang, Wenping Wu
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Publication number: 20130288309Abstract: Variants of a Pseudomonas saccharophila G4-forming amylase (PS4) advantageously can catalyze high temperature saccharification to produce maltotetraose syrup from a starch liquefact or granular starch, e.g., derived from cornstarch. The PS4 variants are useful in a process of saccharification of starch that advantageously produces significant amounts of maltotetraose, which can be used downstream in a process of producing a maltotetraose syrup. In one embodiment, a thermostable PS4 variant is provided that can produce about 40% to about 60% by weight maltotetraose, based on total saccharide content.Type: ApplicationFiled: April 4, 2013Publication date: October 31, 2013Inventors: Gang DUAN, Sung Ho Lee, Ying Qian, Rafael F. Sala, Jayarama Shetty
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Publication number: 20130288307Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation.Type: ApplicationFiled: June 20, 2013Publication date: October 31, 2013Inventor: Marshall Medoff
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Publication number: 20130283795Abstract: A method for the production of alcohol and other bioproducts from power boiler woody biomass extract containing hemicelluloses, with or without combining extract from wood prior to Kraft cooking. The process is integrated with the host Kraft pulp mill plant process to minimize the heat loss from extracting hemicelluloses and the energy used in the process.Type: ApplicationFiled: June 28, 2013Publication date: October 31, 2013Inventors: Theodora RETSINA, Vesa PYLKKANEN
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Publication number: 20130273611Abstract: Compositions and methods are provided for treating lignocellulosic material with a xylanase enzyme having xylanase activity. The enzyme is stable and active at increased pHs and temperatures. The present invention therefore provides methods for hydrolyzing lignocellulosic material, especially cellulose and hemicellulose, which are major components of the cell wall of non-woody and woody plants. The methods for hydrolyzing cellulose and hemicellulose can be used on any plant, wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct.Type: ApplicationFiled: September 8, 2011Publication date: October 17, 2013Applicant: SYNGENTA BIOTECHNOLOGY INC.Inventors: John Steffens, Paul Oeller, Yoshimi Barron
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Publication number: 20130269061Abstract: Genetically engineered plants having altered levels of one or more starch regulation enzymes and a polysaccharide degrading enzyme are provided. Methods of genetically engineering plants to express products altering expression of one or more starch regulation enzymes and polysaccharide degrading enzymes, and genetic constructs are provided. Methods of agricultural processing and animal feed using the genetically engineered plants are described.Type: ApplicationFiled: March 11, 2013Publication date: October 10, 2013Inventors: Philip A. Lessard, Michael Lanahan, Vladimir Samoylov, Oleg Bougri, Jonas Emery, R. Michael Raab, Dongcheng Zhang
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Publication number: 20130266991Abstract: A method produces a concentrated aqueous sugar solution using a cellulose-containing biomass as a raw material, including: (1) hydrolyzing a cellulose-containing biomass to produce an aqueous sugar solution; (2) filtering the aqueous sugar solution obtained in (1) through a microfiltration membrane and/or an ultrafiltration membrane, and recovering an aqueous sugar solution from the permeate side; and (3) filtering the aqueous sugar solution obtained in (2) through a reverse osmosis membrane, recovering a permeate from the permeate side and recovering a concentrated aqueous sugar solution from the feed side; wherein at least a part of the permeate from the reverse osmosis membrane is used as a hydrothermal treatment liquid, biomass-suspending, liquid, enzyme-diluting liquid, acid-diluting liquid and/or alkali-diluting liquid in (1).Type: ApplicationFiled: December 7, 2011Publication date: October 10, 2013Applicant: TORAY Industries, Inc.Inventors: Satoko Kanamori, Masayuki Hanakawa, Hiroyuki Kurihara, Norihiro Takeuchi, Atsushi Minamino
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Publication number: 20130267002Abstract: The present invention relates to polypeptides having glucoamylase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: June 12, 2013Publication date: October 10, 2013Inventors: Sara Landvik, Marc Dominique Morant, Keiichi Ayabe, Guillermo Coward-Kelly
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Patent number: 8551747Abstract: A process for pretreating a lignocellulosic plant raw material in order to obtain a pretreated material that is capable of being hydrolyzed and fermented for the production of bioethanol, includes the following successive steps: (i) destructuring the lignocellulosic plant raw material by placing it in the presence of a mixture containing formic acid and water, at a reaction temperature between 95° C. and 110° C.; (ii) then, at atmospheric pressure and prior to any hydrolysis then fermentation action, separating: on the one hand, the solid phase, mainly composed of the cellulose, constituting a first co-substrate, capable of then being hydrolyzed and fermented for the production of bioethanol; and on the other hand, the liquid phase, containing, in particular in aqueous solution, the formic acid, the lignins, and the hemicelluloses, constituting a second co-substrate, capable of then being hydrolyzed and fermented for the production of bioethanol.Type: GrantFiled: January 22, 2009Date of Patent: October 8, 2013Assignee: Campagnie Industrielle de la Matiere Vegetale CIMVInventors: Michel Delmas, Bouchra Benjelloun Mlayah