Oxidoreductase (1. ) (e.g., Luciferase) Patents (Class 435/189)
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Patent number: 10961560Abstract: A mutated tryptophan oxidase suitable for practical implementation is described herein. Specifically, a mutated tryptophan oxidase wherein at least one amino acid residue of a wild-type tryptophan oxidase is mutated and, as a result, has higher tryptophan oxidase activity and/or stability as compared to the wild-type tryptophan oxidase. The mutated tryptophan oxidase can be derived from a wild-type tryptophan oxidase having at least one of Motifs (2), (3), (5), (7), (9), (11), (13), and (14), and at least one amino acid residue in any of these motifs can have mutation. The mutated tryptophan oxidase also can have a mutation of one or more amino acid residues in an amino acid sequence represented by SEQ ID NO: 2 and a sequence homologous thereto.Type: GrantFiled: March 6, 2019Date of Patent: March 30, 2021Assignee: AJINOMOTO CO., INC.Inventors: Hiroki Yamaguchi, Kazutoshi Takahashi, Moemi Tatsumi
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Patent number: 10851397Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.Type: GrantFiled: June 28, 2019Date of Patent: December 1, 2020Assignee: Codexis, Inc.Inventors: Jack S Liang, Stephan Jenne, Emily Mundorff, Rama Voladri, James J. Lalonde, Gjalt W. Huisman
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Patent number: 10851351Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.Type: GrantFiled: February 25, 2019Date of Patent: December 1, 2020Assignee: Codexis, Inc.Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
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Patent number: 10851141Abstract: The present invention provides a mutant beetle luciferase and the like, having mutation in which the amino acid corresponding to valine at position 288 in the amino acid sequence of wild-type Photinus pyralis luciferase is isoleucine, leucine or phenylalanine, mutation in which the amino acid corresponding to leucine at position 376 in the aforementioned sequence is proline, mutation in which the amino acid corresponding to glutamic acid at position 455 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, or mutation in which the amino acid corresponding to glutamic acid at position 488 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, in the amino acid sequence encoding a wild-type beetle luciferase, and characterized in that a luminescence intensity due to a luciferin-luciferase luminescence reaction in a 0.9% by mass NaCl solution is 50% or more of that in a NaCl-free solution.Type: GrantFiled: June 19, 2017Date of Patent: December 1, 2020Assignee: DKK-TOA CorporationInventors: Kenichi Noda, Satoshi Yawata, Ai Shimomura
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Patent number: 10813324Abstract: The subject invention concerns materials and methods for modulating seed size in plants. In one embodiment, seed size is decreased relative to wild type seed by inhibiting or knocking out expression of a sorbitol dehydrogenase (Sdh) gene or the gene product thereof. In another embodiment, seed size is increased relative to wild type seed by increasing expression of an Sdh gene or the gene product thereof. The subject invention also concerns materials and methods for modulating seed number or sugar content in plants. In one embodiment, seed number or sugar content is increased relative to wild type seed by inhibiting or knocking out expression of a sorbitol dehydrogenase (Sdh) gene or the gene product thereof. In another embodiment, seed number or sugar content is decreased relative to wild type seed by increasing expression of an Sdh gene or the gene product thereof.Type: GrantFiled: May 19, 2008Date of Patent: October 27, 2020Assignee: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Karen Elaine Koch, Sylvia Morais De Sousa
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Patent number: 10808215Abstract: The present disclosure provides a culture device for enumerating colonies of sulfate-reducing microorganisms. The device includes a body having a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed therebetween. The growth compartment has a perimeter and an opening. A portion of the perimeter is defined by a waterproof seal. The portion can include >50% of the perimeter. Disposed in the growth compartment are a dry cold water-soluble gelling agent, a dry culture medium selected to facilitate growth of a sulfate-reducing bacterium or indicator reagent for detecting hydrogen sulfide production by a sulfate-reducing bacterium, and a dry first oxygen-scavenging reagent.Type: GrantFiled: August 19, 2015Date of Patent: October 20, 2020Assignee: 3M Innovative Properties CompanyInventors: Evan D. Brutinel, Jason W. Bjork, Adam J. Stanenas
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Patent number: 10750741Abstract: A microbial oil comprising dihomo-?-linolenic acid as a constituent fatty acid of an oil, the microbial oil has a content, in terms of a weight ratio of arachidonic acid relative to dihomo-?-linolenic acid (arachidonic acid/dihomo-?-linolenic acid) of less than 1/13. Preferably, the microbial oil has a triglyceride content of greater than or equal to 70% by weight, and a saturated fatty acid content of less than or equal to 40% by weight. Moreover, a lower alcohol ester of dihomo-?-linolenic acid or a free fatty acid of dihomo-?-linolenic acid obtained from the microbial oil is provided.Type: GrantFiled: October 25, 2017Date of Patent: August 25, 2020Assignee: NIPPON SUISAN KAISHA, LTD.Inventors: Seizo Sato, Takuro Fukae, Naomi Ohtsuka, Hideaki Yamaguchi, Rie Ikeda
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Patent number: 10751393Abstract: The present invention relates to a Bacillus amyloliquefaciens strain that produces superoxide dismutase (SOD), and also relates to an antioxidant, anti-inflammatory pharmaceutical composition and a pharmaceutical composition and food composition for preventing or treating hyperlipidemia, which include a superoxide dismutase produced by the Bacillus amyloliquefaciens strain. The compositions of the present invention exhibit excellent effects without causing side effects, and may thus be used as functional raw materials or products having an enhanced activity of preventing or treating inflammation, cancer or hyperlipidemia in the pharmaceutical drug, food, cosmetic and livestock fields.Type: GrantFiled: March 7, 2018Date of Patent: August 25, 2020Assignee: GENOFOCUS INC.Inventors: Do Young Yum, Jeong Hyun Kim, Jae Gu Pan, Eui Joong Kim, Taek Ho Yang, Ji Eun Kang, Soo Young Park, Hyun Do Kim
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Patent number: 10704070Abstract: The present disclosure relates to the biosynthesis of indigoid dye precursors and their conversion to indigoid dyes. Specifically, the present disclosure relates to methods of using polypeptides to produce indigoid dye precursors from indole feed compounds, and the use of the indigoid dye precursors to produce indigoid dyes.Type: GrantFiled: March 3, 2016Date of Patent: July 7, 2020Assignee: The Regents of the University of CaliforniaInventors: John Eugene Dueber, Zachary Nicholas Russ, Tammy Melody Hsu, Terry Don Johnson, Jr., Bernardo Cervantes, Ramya Lakshmi Prathuri, Shyam Pravin Bhakta, Arthur Muir Fong, III, Luke Nathaniel Latimer
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Patent number: 10683546Abstract: An ex vivo method and a kit for predicting the response to a treatment with glucocorticoids (GC) in patients affected by inflammatory diseases, based on quantification of fold change ratio in GR isoform levels.Type: GrantFiled: May 24, 2016Date of Patent: June 16, 2020Assignee: UNIVERSIDAD DE CHILEInventors: Cristhian Alejandro Urzua Salinas, Irmgadt Annelise Goecke Sariego
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Patent number: 10619176Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: GrantFiled: November 19, 2018Date of Patent: April 14, 2020Assignee: Codexis, Inc.Inventors: Christopher K. Savile, Emily Mundorff, Jeffrey C. Moore, Paul N. Devine, Jacob M. Janey
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Patent number: 10604761Abstract: The present invention provides host cells for use in an inducible coexpression system that is capable of controlled induction of expression of each gene product.Type: GrantFiled: February 16, 2017Date of Patent: March 31, 2020Assignee: AbSci LLCInventors: Sean McClain, Mark Valasek
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Patent number: 10590396Abstract: The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine.Type: GrantFiled: June 11, 2019Date of Patent: March 17, 2020Assignee: Codexis, Inc.Inventors: Oscar Alvizo, Steven J. Collier, Hans-Georg Joerg Hennemann, Seong Ho Oh, Wenjuan Zha
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Patent number: 10570408Abstract: This invention provides recombinant DNA constructs, transgenic plant nuclei and cells with such recombinant DNA construct for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.Type: GrantFiled: December 5, 2017Date of Patent: February 25, 2020Assignee: Monsanto Technology LLCInventors: Molian Deng, Zhidong Xie
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Patent number: 10563230Abstract: The present disclosure relates to a multi-enzyme conjugate, a method for preparing the same and a method for preparing an organic compound using the same. More particularly, a multi-enzyme conjugate exhibiting improved catalytic efficiency over respective free enzymes using site-specific incorporation of a clickable non-natural amino acid into the enzymes and two compatible click reactions, a method for preparing the same and a method for preparing an organic compound using the same may be provided.Type: GrantFiled: May 26, 2017Date of Patent: February 18, 2020Assignee: GWANGJU INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Inchan Kwon, Jinhwan Cho, Sung In Lim
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Patent number: 10544400Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: GrantFiled: July 11, 2018Date of Patent: January 28, 2020Assignee: Codexis, Inc.Inventors: Michael A. Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga K. Modukuru, Emily Mundorff, Derek J. Smith, Shiwei Song, Wan Lin Yeo
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Patent number: 10533153Abstract: The present invention relates to a process for the production of squalene and/or sterol in high amounts using an alkaline solution and an organic lysis solvent at high temperature and high pressure for effectively lysing yeast cells and extracting squalene and/or sterol into an organic extraction solvent, thus obtaining squalene and/or sterolin high amount and of high purity.Type: GrantFiled: March 11, 2014Date of Patent: January 14, 2020Assignee: SANOFI-AVENTIS DEUTSCHLAND GMBHInventors: Andreas Haubrich, Gerhard Korb, Jean-Francois Trotzier
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Patent number: 10377722Abstract: An object of the present invention is to provide a novel method capable of producing rosuvastatin calcium and intermediates therefor efficiently, inexpensively and with high purity. The present invention provides a method of efficiently producing rosuvastatin calcium and intermediates therefor having a high purity at an industrial scale, without using an extremely low temperature reaction or a special asymmetric catalyst.Type: GrantFiled: May 19, 2017Date of Patent: August 13, 2019Assignee: API CORPORATIONInventors: Naoyuki Watanabe, Tomoko Maeda, Yasumasa Dekishima, Hiroshi Kawabata, Masaki Nagahama, Kosuke Ito
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Patent number: 10378023Abstract: The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.Type: GrantFiled: August 4, 2016Date of Patent: August 13, 2019Assignee: Monsanto Technology LLCInventors: Artem G. Evdokimov, Clayton T. Larue, Farhad Moshiri, Joel E. Ream, Xuefeng Zhou
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Patent number: 10370677Abstract: The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.Type: GrantFiled: July 29, 2016Date of Patent: August 6, 2019Assignee: Monsanto Technology LLCInventors: Artem G. Evdokimov, Clayton T. Larue, Farhad Moshiri, Joel E. Ream, Xuefeng Zhou
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Patent number: 10358631Abstract: The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine.Type: GrantFiled: November 14, 2017Date of Patent: July 23, 2019Assignee: Codexis, Inc.Inventors: Oscar Alvizo, Steven J. Collier, Hans-Georg Joerg Hennemann, Seong Ho Oh, Wenjuan Zha
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Patent number: 10264807Abstract: The invention relates to fusarium toxin-cleaving polypeptide variants of a fusarium toxin carboxyl esterase with the SEQ ID NO:46. Each of the polypeptide variants has an amino acid sequence shortened by 47 amino acids at the N terminus, and the amino acid sequences have at least 70%, preferably 80%, in particular 100%, sequence identity, namely SEQ ID NO:1, to the amino acid sequence portion 48-540 of the SEQ ID NO:46. The invention also relates to isolated polynucleotides which code for the polypeptide variants, to a fusarium toxin-cleaving additive containing at least one polypeptide variant and optionally at least one auxiliary agent, to the use of the polypeptide variants or the additive, and to a method for hydrolytically cleaving at least one fusarium toxin.Type: GrantFiled: February 24, 2015Date of Patent: April 23, 2019Assignee: ERBER AKTIENGESELLSCHAFTInventors: Markus Aleschko, Corinna Kern, Dieter Moll, Eva Maria Binder, Gerd Schatzmayr
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Patent number: 10253303Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.Type: GrantFiled: September 10, 2018Date of Patent: April 9, 2019Assignee: Codexis, Inc.Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
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Patent number: 10246687Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.Type: GrantFiled: July 10, 2018Date of Patent: April 2, 2019Assignee: Codexis, Inc.Inventors: Emily Mundorff, Erik Jan De Vries
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Patent number: 10239922Abstract: The present disclosure pertains to methods of producing recombinant peptides that contain between 10 and 200 amino acid residues using novel carrier proteins derived from superfolder green fluorescent protein and its mutants.Type: GrantFiled: June 29, 2017Date of Patent: March 26, 2019Assignee: Suzhou Kunpeng Biotech Co., Ltd.Inventors: Wenshe Liu, Wei Wan
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Patent number: 10202627Abstract: An object of the present invention is to provide enzymes and a DNA encoding the enzymes that are involved in biosynthesis of trehangelin which has the potential to be a therapeutic agent for photosensitivity disorder and cosmetics, and to provide a method for producing trehangelin by utilizing the enzymes and a recombinant microorganism. The present invention is directed to a protein having an amino acid sequence of SEQ ID NO: 3, 5, 7 or 9, or a protein having an amino acid sequence of SEQ ID NO: 3, 5, 7 or 9 in which one to several amino acids are deleted, substituted, added and/or inserted or an amino acid sequence having 60% or more homology with the amino acid sequence of SEQ ID NO: 3, 5, 7 or 9 and having an enzyme activity involved in biosynthesis of trehangelin; and a DNA encoding said protein.Type: GrantFiled: February 28, 2017Date of Patent: February 12, 2019Assignees: The Kitasato Institute, Nagase & Co., Ltd.Inventors: Satoshi Omura, Yoko Takahashi, Takuji Nakashima, Yuki Inahashi
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Patent number: 10202585Abstract: Improved matrix or hydrogel that is formed by enzymatic crosslinking of polymers wherein the crosslinking enzyme molecules have been modified for the purpose of improving the crosslinking density, mechanical properties, or other properties of the matrix, and/or to provide improved control over the rate and/or extent of crosslinking, wherein the enzyme molecules are modified to alter the perceived volume of the enzyme molecules in the crosslinked matrix being formed. Methods of production and of use are also provided.Type: GrantFiled: April 25, 2015Date of Patent: February 12, 2019Assignee: LIFEBOND LTDInventors: Guy Tomer, Orahn Preiss-Bloom
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Patent number: 10184116Abstract: A novel luciferase that distinct from conventional luciferase has been desired. A luciferase mutant comprising the amino acid sequence of SEQ ID NO: 2 substituted at tyrosine at the position of 138, and at least 3 positions selected from the group consisting of isoleucine at the position of 90, proline at the position of 115, glutamine at the position of 124, and asparagine at the position of 166.Type: GrantFiled: March 9, 2015Date of Patent: January 22, 2019Assignee: JNC CORPORATIONInventors: Satoshi Inouye, Junichi Sato
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Patent number: 10100287Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.Type: GrantFiled: September 21, 2017Date of Patent: October 16, 2018Assignee: Codexis, Inc.Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
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Patent number: 10093905Abstract: The invention relates to ketoreductases and the use thereof. The ketoreductases of the invention are particularly useful for enzymatically catalyzing the reduction of ketones to chiral secondary alcohols.Type: GrantFiled: April 17, 2015Date of Patent: October 9, 2018Assignee: C-LEcta GmbHInventors: Ramona Schmiedel, Andreas Vogel, Sabrina Koepke, Rico Czaja, Claudia Feller, Hedda Merkens, Kamila Rzeznicka, Daniel Schwarze, Thomas Greiner-Stoeffele, Andreas Petri, Marc Struhalla
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Patent number: 10053673Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: GrantFiled: April 4, 2017Date of Patent: August 21, 2018Assignee: Codexis, Inc.Inventors: Michael A. Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga K. Modukuru, Emily Mundorff, Derek J. Smith, Shiwei Song, Wan Lin Yeo
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Patent number: 10047348Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.Type: GrantFiled: September 21, 2017Date of Patent: August 14, 2018Assignee: Codexis, Inc.Inventors: Emily Mundorff, Erik Jan De Vries
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Patent number: 9988609Abstract: Fructosyl amino acid oxidase is provided, which has an amino acid sequence as shown in SEQID. No. 1 or fructosyl amino acid oxidase having a homology of more than 80% with this amino acid sequence, on a corresponding site of an amino acid selected from following (a) to (f), having one or more amino acid residues conducting a substitution, obtained fructosyl amino acid oxidase having a higher thermostability: (a) 59-site glutamic acid, (b) 98-site glutamic acid, (c) 225-site glycine, (d) 277-site lysine, (e) 285-site glutamic acid, and (f) 355-site aspartic acid. The method for preparing the above oxidase and the test kit containing the enzyme for determining glycated albumin are also provided.Type: GrantFiled: December 11, 2014Date of Patent: June 5, 2018Assignee: MEDICAL SYSTEM BIOTECHNOLOGY CO., LTD.Inventors: Bingde Zou, Jihua Zou, Yi Wang, Jianghua Jia
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Patent number: 9976164Abstract: The present invention relates to a method for producing a protein of interest containing one or more disulfide bonds in its native state. The method comprises that a prokaryotic host cell is genetically engineered to express the protein of interest and a sulfhydryl oxidase in the cytoplasm of the host cell. The protein of interest is formed in a soluble form and contains disulfide bonds due to the presence of the sulfhydryl oxidase in the cytoplasm of said host cell. The present invention relates also to a prokaryotic host cell and a vector system for producing a protein of interest containing natively folded disulfide bonds.Type: GrantFiled: December 9, 2015Date of Patent: May 22, 2018Assignee: University of OuluInventor: Lloyd Ruddock
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Patent number: 9963720Abstract: This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.Type: GrantFiled: August 1, 2016Date of Patent: May 8, 2018Assignee: California Institute of TechnologyInventors: Frances Arnold, Peter Meinhold, Matthew W. Peters, Rudi Fasan, Mike M. Y. Chen
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Patent number: 9951102Abstract: An isolated peptide or fusion having the peptide useful to interfere with the phosphorylation of Nox1, block or inhibit binding of NoxA1 activation domain (AD) to Nox1, and/or prevent or inhibit assembly of the p47phox/NoxA1 complex with Nox1, or a vector encoding the peptide or fusion thereof, and methods of using the peptide, fusion or vector are provided.Type: GrantFiled: April 17, 2015Date of Patent: April 24, 2018Assignee: University of Iowa Research FoundationInventors: Francis J. Miller, Jr., Jennifer Streeter
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Patent number: 9896674Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.Type: GrantFiled: January 21, 2016Date of Patent: February 20, 2018Assignee: DANISCO US INCInventors: Anthony Day, Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe, Mats Sandgren, Andrew Shaw, Jerry Stahlberg
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Patent number: 9868941Abstract: A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which glutamic acid at position 4, arginine at position 11, leucine at position 18 and valine at position 27 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.Type: GrantFiled: April 14, 2015Date of Patent: January 16, 2018Assignee: JNC CORPORATIONInventors: Satoshi Inouye, Yuiko Miura, Junichi Sato
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Patent number: 9861602Abstract: One embodiment described herein is related to methods and compositions, such as nutraceutical formulations and dietary supplements, comprising C16:1n7-palmitoleate or derivatives thereof. The methods and compositions comprising C16:1n7-palmitoleate, or derivatives thereof, safely and effectively prevent or mitigate manifestations of cardiovascular disease, including coronary artery disease and the accumulation of cholesterol or lipid deposits in the blood vessels of a subject.Type: GrantFiled: April 1, 2014Date of Patent: January 9, 2018Assignee: TERSUS LIFE SCIENCES, LLCInventor: Jeffrey Green
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Patent number: 9834802Abstract: Methods for the production of L-glufosinate (also known as phosphinothricin or (S)-2-amino-4-(hydroxy(methyl)phosphonoyl)butanoic acid) are provided. The methods comprise a two-step process. The first step involves the oxidative deamination of D-glufosinate to PPO (2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid). The second step involves the specific amination of PPO to L-glufosinate, using an amine group from one or more amine donors. By combining these two reactions, the proportion of L-glufosinate in a mixture of L-glufosinate and D-glufosinate can be substantially increased.Type: GrantFiled: February 28, 2017Date of Patent: December 5, 2017Assignee: AgriMetis, LLCInventors: Brian Michael Green, Michelle Lorraine Gradley
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Patent number: 9828588Abstract: The present invention provides a stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the cell is increased 10% as compared to the amount of fully carboxylated VKD protein produced in the cell in the absence of the recombinant VKOR and the recombinant VKGC.Type: GrantFiled: September 25, 2014Date of Patent: November 28, 2017Assignee: The University of North Carolina at Chapel HillInventors: Darrel W. Stafford, Tao Li
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Patent number: 9820484Abstract: A microbial oil comprising dihomo-?-linolenic acid as a constituent fatty acid of an oil, the microbial oil has a content, in terms of a weight ratio of arachidonic acid relative to dihomo-?-linolenic acid (arachidonic acid/dihomo-?-linolenic acid) of less than 1/13. Preferably, the microbial oil has a triglyceride content of greater than or equal to 70% by weight, and a saturated fatty acid content of less than or equal to 40% by weight. Moreover, a lower alcohol ester of dihomo-?-linolenic acid or a free fatty acid of dihomo-?-linolenic acid obtained from the microbial oil is provided.Type: GrantFiled: December 4, 2014Date of Patent: November 21, 2017Assignee: NIPPON SUISAN KAISHA, LTD.Inventors: Seizo Sato, Takuro Fukae, Naomi Ohtsuka, Hideaki Yamaguchi, Rie Ikeda
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Patent number: 9783859Abstract: A method of detecting and quantifying various enzymatic activities using a constructed artificial genetic circuit GESS (genetic enzyme screening system) for sensing phenolic compounds and a method of screening a trace of activities of target enzymes from a metagenome using the artificial genetic circuit, thereby securing target enzyme genes. When the method for screening and quantifying target enzymatic activity is used, useful genes can be screened from various genetic communities, including environmental or metagenomic libraries, at a single cell level in high throughput (million/day). Further, the sensitivity of the genetic circuit to phenol derivatives and the expression thereof can be controlled, and thus the genetic circuit can rapidly sense and quantify various enzymatic activities. Thus, the method can be advantageously used in the protein engineering technology for enzyme modification.Type: GrantFiled: June 8, 2010Date of Patent: October 10, 2017Assignee: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGYInventors: Seung Goo Lee, Eugene Rha, Su Lim Choi, Jae Jun Song, Jong Hyun Choi, Hee Sik Kim
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Patent number: 9765373Abstract: The present invention relates to polypeptides having cellulolytic enhancing activity, catalytic domains, and carbohydrate binding domains, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding domains.Type: GrantFiled: December 13, 2013Date of Patent: September 19, 2017Assignees: Novozymes A/S, Novozymes, Inc.Inventors: Kirk Schnorr, Tarana Shaghasi, Matt Sweeney
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Patent number: 9732328Abstract: The present invention encompasses modified luciferases, methods for making modified luciferases, and assays utilizing modified luciferases. Modified luciferases of the invention show increased activity over wildtype luciferases and also show increased stability of signal. The present invention also encompasses multiplex assays utilizing multiple luciferases with different emission spectra.Type: GrantFiled: January 20, 2016Date of Patent: August 15, 2017Assignee: Targeting SystemsInventor: Rampyari Walia
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Patent number: 9714432Abstract: The present invention provides use of a plant gene JAZ5a for improving drought-resistance of a plant. It further provides a method for improving the drought-resistance of a plant, comprising enhancing the expression or activity of Jaz5A in said plant.Type: GrantFiled: July 5, 2012Date of Patent: July 25, 2017Assignee: Keygene N.V.Inventor: Adrianus Johannes Van Tunen
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Patent number: 9663772Abstract: The present invention relates to recombinant Trichoderma host cells producing Aspergillus fumigatus cellulolytic enzyme compositions and methods of producing and using the compositions.Type: GrantFiled: August 23, 2012Date of Patent: May 30, 2017Assignee: NOVOZYMES, INC.Inventors: Jeffrey Shasky, Brett McBrayer
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Patent number: 9662515Abstract: The invention relates to a process for the production of 4-aminobutyric acid, also referred to as gamma-aminobutyric acid, in short GABA, to a process for the production of an extract comprising 4-aminobutyric acid, to a process for the preparation of a cosmetic formulation comprising 4-aminobutyric acid, and to the use of Cyanidium caldarium cells or cell components in cosmetic applications.Type: GrantFiled: July 20, 2012Date of Patent: May 30, 2017Assignee: EVONIK DEGUSSA GMBHInventors: Tim Köhler, Jennifer Schild, Matthias Mentel, Peter Lersch, Mike Farwick, Christian Weitemeyer
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Patent number: 9650625Abstract: A pectinase having improved thermostability is disclosed. The pectinase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of serine at position 181 with phenylalanine, methionine or leucine.Type: GrantFiled: October 26, 2016Date of Patent: May 16, 2017Assignee: DONGGUAN APAC BIOTECHNOLOGY CO., LTD.Inventors: Rey-Ting Guo, Ya-Shan Cheng, Jian-Wen Huang, Tzu-Hui Wu, Hui-Lin Lai, Cheng-Yen Lin, Tsung-Yu Ko
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Patent number: 9644189Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: GrantFiled: June 10, 2016Date of Patent: May 9, 2017Assignee: Codexis, Inc.Inventors: Michael A. Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga K. Modukuru, Emily Mundorff, Derek J. Smith, Shiwei Song, Wan Lin Yeo