Oxidoreductase (1. ) (e.g., Luciferase) Patents (Class 435/189)
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Patent number: 9631002Abstract: The present invention provides methods and compositions for the production of vitamin K dependent proteins.Type: GrantFiled: December 21, 2011Date of Patent: April 25, 2017Assignee: The University of North Carolina at Chapel HillInventors: Jianke Tie, Darrel W. Stafford
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Patent number: 9630988Abstract: The present invention provides a method for preparing a composition comprising highly concentrated antibodies by ultrafiltration in batch concentration mode having a first constant feed rate step and a second controlled feed rate step.Type: GrantFiled: August 31, 2012Date of Patent: April 25, 2017Assignees: Chugai Seiyaku Kabushiki Kaisha, Genentech, Inc.Inventors: Kelby Lau, Jean Bender, Saeko Tanaka, Rumiko Wakayama, Hidenari Yamada, Tomonori Isoda, Masayoshi Oh-Eda
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Patent number: 9611503Abstract: The objective of this invention is to create a double thyA folA knockout Escherichia coli (E. coli) strain for antifolate screening against DHFR of malaria and other parasites. This strain is used together with a plasmid expressing DHFR-TS from the desired pathogenic organism, which constitutes an anti-DHFR assay against the pathogenic organism of interest. The benefit of this invention is that there is no interference from either host DHFR or trimethoprim, a bacterial DHFR inhibitor. This tool is easy to use and maintain. It provides quick and reliable results as compared with conventional anti-malarial and anti-parasitic assays. This invention should facilitate discovery of new anti-DHFR compounds against malaria and other parasitic diseases.Type: GrantFiled: February 9, 2012Date of Patent: April 4, 2017Assignee: National Science and Technology Development AgencyInventors: Nattida Suwanakitti, Sastra Chaotheing, Yongyuth Yuthavong, Sumalee Kamchonwongpaisan
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Patent number: 9506104Abstract: There has been a demand for a codon-optimized gene for the mutated catalytic domain of Oplophorus luciferase, which is capable of efficiently expressing a protein both in a cultured animal cell and Escherichia coli. There has also been a demand for a substrate coelenterazine analog showing a higher activity than that of native 19 kDa protein. The invention provides a polynucleotide comprising a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2. According to the invention, bis-coelenterazine is used as a substrate coelenterazine analog suitable for the photoprotein encoded by the polynucleotide comprising the polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2.Type: GrantFiled: June 24, 2016Date of Patent: November 29, 2016Assignee: JNC CORPORATIONInventors: Satoshi Inouye, Junichi Sato
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Patent number: 9506042Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity, specific activity, thermal stability, and the like, and that is suitable for use in SMBG sensors. The present invention provides a purified polypeptide comprising an amino acid sequence having at least 80% identity to the sequence of SEQ ID NO: 1 and having glucose dehydrogenase activity.Type: GrantFiled: December 29, 2014Date of Patent: November 29, 2016Assignee: Toyobo Co., Ltd.Inventors: Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroaki Kitazawa, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
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Patent number: 9504271Abstract: A method for sterilizing microbial cells is provided. According to the method, microbial cells or a culture containing microbial cells are treated with a polyethylene glycol-based nonionic surfactant so that almost all of the microbial cells are sterilized while the enzyme activity expressed in the microbial cells is maintained at a high level. A method for sterilizing microbial cells and a material containing the sterilized microbial cells, in which the microbial cells are sterilized using a polyethylene glycol-based nonionic surfactant, can be used for foods so that the microbial cells are sterilized to be used in food production. Further, a material containing sterilized microbial cells can be used in processes for preparing tagatose, in which Corynebacterium genus microbial cells that produce Galactose and/or Arabinose isomerase are sterilized using a polyethylene glycol-based nonionic surfactant.Type: GrantFiled: June 20, 2014Date of Patent: November 29, 2016Assignee: CJ CHEILJEDANG CORPORATIONInventors: Jin Ha Kim, Young Mi Lee, Seong Bo Kim, Taek Beom Kim, Yang Hee Kim, Seung Won Park
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Patent number: 9476036Abstract: The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.Type: GrantFiled: August 23, 2012Date of Patent: October 25, 2016Assignee: Novozymes, Inc.Inventors: Jeffrey Shasky, Amanda Fischer, Suchindra Maiyuran
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Patent number: 9453237Abstract: The present invention relates to means and methods for modifying the expression of a component in the carotenoid biosynthetic pathway to modify the production of carotenoid towards accumulation of at least one of phytoene, phytofluene, zeta carotene or combinations thereof. The present invention further relates to genetically altered organisms having elevated content of these carotenoids. The present invention further relates to genetically altered organisms having elevated content of these carotenoids, particularly in chromoplast-containing cells.Type: GrantFiled: March 9, 2011Date of Patent: September 27, 2016Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.Inventors: Joseph Hirschberg, Dani Zamir
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Patent number: 9441236Abstract: Transgenic plants, and a method for making the same, wherein genes encoding the enzyme luciferase and its corresponding substrate luciferin are incorporated into a native plant genome. Once transformed into plant cells, these genes may be regulated such that under certain endogenous or exogenous conditions, their expression in the mature plant results in bioluminescence. Different luciferin/luciferase complexes and/or mechanisms of regulation may be utilized for these transgenic plants, depending on a variety of factors such as plant species and the circumstances under which a biolmuniescent reaction is desired. Phototransformation may be utilized to vary the wavelength of light emitted from the mature plant.Type: GrantFiled: September 25, 2014Date of Patent: September 13, 2016Inventor: Bruce Eric Hudkins
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Patent number: 9422530Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.Type: GrantFiled: August 12, 2015Date of Patent: August 23, 2016Assignee: Codexis, Inc.Inventors: Emily Mundorff, Erik Jan De Vries
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Patent number: 9416350Abstract: An object of the present invention is to provide a method for converting the coenzyme dependency of enzymes of the medium-chain dehydrogenase/reductase (MDR) family. A further object of the present invention is to provide enzyme variants of the MDR family whose coenzyme dependency is converted by the conversion method and a method for enzymatically producing optically active alcohols using the enzymes. The present inventors developed a novel enzyme conversion method for converting the coenzyme dependency of enzymes of the MDR family, rationally designed enzyme variants that are altered by the enzyme conversion method to be able to use NADPH as a coenzyme from a useful enzyme of the MDR family that uses NADH as a coenzyme, and actually provide variants having such an ability.Type: GrantFiled: June 27, 2012Date of Patent: August 16, 2016Assignee: KANEKA CORPORATIONInventors: Shinichi Yoshida, Shunichi Taira, Masakatsu Nishihachijyo, Masutoshi Nojiri, Shigeru Kawano
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Patent number: 9404145Abstract: There has been a demand for a codon-optimized gene for the mutated catalytic domain of Oplophorus luciferase, which is capable of efficiently expressing a protein both in a cultured animal cell and Escherichia coli. There has also been a demand for a substrate coelenterazine analog showing a higher activity than that of native 19 kDa protein. The invention provides a polynucleotide comprising a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2. According to the invention, bis-coelenterazine is used as a substrate coelenterazine analog suitable for the photoprotein encoded by the polynucleotide comprising the polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2.Type: GrantFiled: March 11, 2016Date of Patent: August 2, 2016Assignee: JNC CORPORATIONInventors: Satoshi Inouye, Junichi Sato
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Patent number: 9394513Abstract: For the purpose of providing a method of safely and inexpensively producing a fermented plant extract containing an immunopotentiator at a high concentration, the method for fermentation and culture of the present invention ferments a plant component such as wheat flour using Pantoea agglomerans which is a gram negative bacterium which lives in a symbiotic relationship with a plant such as wheat and apple. It becomes possible to remarkably augment an immunopotentiation action which the plant has. In addition, these are not contaminated with impurities derived from animal components, and thus these are highly safe.Type: GrantFiled: November 15, 2011Date of Patent: July 19, 2016Assignees: BIOMEDICAL RESEARCH GROUP INC.Inventors: Gen-Ichiro Soma, Chie Kohchi, Hiroyuki Inagawa, Takashi Nishizawa, Yukinori Takahashi
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Patent number: 9388391Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: GrantFiled: April 22, 2015Date of Patent: July 12, 2016Assignee: Codexis, Inc.Inventors: Michael A. Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga K. Modukuru, Emily Mundorff, Derek J. Smith, Shiwei Song, Wan Lin Yeo
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Patent number: 9365878Abstract: The present disclosure relates to biocatalysts and its uses for the efficient preparation of eslicarbazepine, eslicarbazepine acetate, and analogs thereof.Type: GrantFiled: July 2, 2015Date of Patent: June 14, 2016Assignee: Codexis, Inc.Inventors: Anupam Gohel, Derek J. Smith, Brian Wong, Joly Sukumaran, Wan Lin Yeo, Steven J. Collier, Scott Novick
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Patent number: 9353401Abstract: The present invention encompasses modified luciferases, methods for making modified luciferases, and assays utilizing modified luciferases. Modified luciferases of the invention show increased activity over wildtype luciferases and also show increased stability of signal. The present invention also encompasses multiplex assays utilizing multiple luciferases reporters with different emission spectra and different substrates for simultaneous luciferase measurements.Type: GrantFiled: August 27, 2010Date of Patent: May 31, 2016Assignee: Targeting SystemsInventor: Rampyari Walia
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Patent number: 9353355Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: GrantFiled: August 5, 2015Date of Patent: May 31, 2016Assignee: Codexis, Inc.Inventors: Christopher K. Savile, Emily Mundorff, Jeffrey C. Moore, Paul N. Devine, Jacob M. Janey
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Patent number: 9340769Abstract: The disclosure provides an antigenic composition useful for immunization against P. acnes, K. pneumoniae, S. aureus, or Streptococcus pyogenes. The disclosure provides a method for producing a vaccine for preventing infection and screening agents useful for preventing infection.Type: GrantFiled: December 3, 2009Date of Patent: May 17, 2016Assignee: The Regents of the University of CaliforniaInventors: Richard L. Gallo, Chun-Ming Huang
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Patent number: 9315815Abstract: The invention provides a method of high-throughput sorting of high expression protein-producing cell, which utilizes linking a protein and a transmembrane domain with a self-processing cleavage site and regulating the secretion of the protein or expression of protein on the cell membrane by adding self-processing cleavage enzyme inhibitor. Then, the high expression cell line can be high-throughput sorted by a detection technique. The invention also provides a recombinant nucleotide sequence and a vector used in the method and a cell sorted by the method.Type: GrantFiled: April 28, 2014Date of Patent: April 19, 2016Assignee: Taipei Medical UniversityInventors: Kuo-Hsiang Chuang, Tian-Lu Cheng, I-Shiuan Chiang, Yuan-Chin Hsieh
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Patent number: 9273290Abstract: In order to provide: a phosphite dehydrogenase protein having both improved solubility and improved heat stability; a method for producing a gene encoding the phosphite dehydrogenase protein; a method for producing the phosphite dehydrogenase protein; and use of the phosphite dehydrogenase protein, (i) a phosphite dehydrogenase protein having a specific amino acid sequence and (ii) a gene encoding the phosphite dehydrogenase protein are used.Type: GrantFiled: April 16, 2012Date of Patent: March 1, 2016Assignee: HIROSHIMA UNIVERSITYInventors: Akio Kuroda, Ryuichi Hirota
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Patent number: 9267116Abstract: The present invention relates to a novel amino acid dehydrogenase, DNA encoding the enzyme, and a transformant having the DNA introduced therein. The present invention also relates to a process for producing an L-amino acid, 2-oxo acid or D-amino acid, which includes allowing the amino acid dehydrogenase or a microorganism or transformant capable of producing the enzyme to act on a substrate compound. The amino acid dehydrogenase has good reactivity even with an amino acid or a 2-oxo acid each having a bulky side chain such as an aromatic-ring-containing group, which acids are poorly reactive with conventional amino acid dehydrogenases. The amino acid dehydrogenase enables the inexpensive and highly efficient production of a useful optically active amino acid.Type: GrantFiled: December 8, 2009Date of Patent: February 23, 2016Assignee: KANEKA CORPORATIONInventors: Hiroyuki Kanamaru, Ryuuji Miki, Makoto Ueda, Hironori Nanba, Yoshihiko Yasohara
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Patent number: 9255256Abstract: A bacterial cell containing a functional cytochrome P450 monooxygenase system, said cell comprising a genetic construct capable of expressing a cytochrome P450 and a genetic construct capable of expressing, separately from said cytochrome P450, a cytochrome P450 reductase wherein the N-terminus of the cytochrome P450 and the N-terminus of the cytochrome P450 reductase are each adapted to allow functional coupling of said cytochrome P450 and said cytochrome P450 reductase within said cell. A bacterial cell containing a cytochrome P450 comprising a genetic construct encoding, and capable of expressing, said cytochrome P450 wherein the cytochrome P450 comprises an N-terminal portion which directs the cytochrome P450 to a cellular compartment of membrane of the bacterial cell. The bacterial cells are useful as, for example, bioreactors, in drug testing and mutagenicity testing and as a source of cytochrome P450.Type: GrantFiled: February 18, 2003Date of Patent: February 9, 2016Inventors: Charles Roland Wolf, Thomas Herbert Friedberg, Michael Patrick Pritchard
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Patent number: 9238801Abstract: Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.Type: GrantFiled: March 15, 2013Date of Patent: January 19, 2016Assignee: Butamax Advanced Biofuels LLCInventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
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Patent number: 9220700Abstract: This application describes methods of making and using physiological cysteine solutions useful for reversing a neuromuscular blockade caused by a cysteine-reversible neuromuscular blockade agent, that overcomes problems of cysteine precipitation and dimerization.Type: GrantFiled: August 18, 2010Date of Patent: December 29, 2015Assignee: Cornell UniversityInventors: John J. Savarese, Paul M. Heerdt
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Patent number: 9222109Abstract: The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons.Type: GrantFiled: March 28, 2011Date of Patent: December 29, 2015Assignee: NOVOZYMES A/SInventors: Martin Hofrichter, Katrin Scheibner, René Ullrich, Matthias Kinne, Sebastian Peter, Henrik Lund, Lisbeth Kalum
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Patent number: 9187734Abstract: A glucose dehydrogenase, which is an enzyme that has high substrate specificity, can be produced at a low cost, is not affected by oxygen dissolved in a measurement sample and, in particular, has superior thermal stability is obtained by culturing a microorganism belonging to the genus Burkholderia. A glucose sensor utilizing the glucose dehydrogenase protein is described.Type: GrantFiled: March 6, 2014Date of Patent: November 17, 2015Inventor: Koji Sode
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Patent number: 9169469Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: November 28, 2012Date of Patent: October 27, 2015Assignee: Novozymes A/SInventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
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Patent number: 9127249Abstract: Provided is a novel method for preparing metabolites of atorvastatin using bacterial cytochrome P450, and a composition therefor, and more particularly, a composition for preparing 2-hydroxylated product of 4-hydroxylated product from atorvastatin including bacterial cytochrome P 450 BM3 (CYP102A1), CYP102A1 mutants, and chimeras derived from the CYP102A1 mutants, a kit therefor, and a method for preparing thereof.Type: GrantFiled: September 27, 2012Date of Patent: September 8, 2015Assignee: INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITYInventors: Chul-Ho Yun, Dong-Hyun Kim, Ji-Yeon Kang, Sun-Ha Park, Sang-Hoon Ryu
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Patent number: 9127301Abstract: Described herein is a rapid coupled enzymatic assay to detect polycyclic aromatic hydrocarbon (PAH) compounds in samples. The method uses a detection composition that includes: a buffered solution comprising NADH; naphthalene dioxygenase, naphthalene 1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase, and 1,2-dihydroxynaphthalene dioxygenase. The presence of PAH is determined by noting a color change of the detection composition, either visually or spectrophotometrically.Type: GrantFiled: November 21, 2013Date of Patent: September 8, 2015Assignee: BIOO SCIENTIFIC CORPORATIONInventors: Joseph F. Krebs, John Duvall-Jisha, Sol Resnick
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Patent number: 9115364Abstract: The present invention relates to a method of producing a target protein, which method comprises expressing said protein in a host cell which contains a nucleic acid molecule which encodes a chimeric protein, said chimeric protein comprising a signal peptide from a non-mammalian bulk-secreted protein and said target protein; nucleic acids, vectors, host cells and kits for carrying out the method are also described.Type: GrantFiled: November 28, 2011Date of Patent: August 25, 2015Assignee: PROLUME LTD.Inventors: John Hesketh, Hanne Ravneberg, Christine Gjerdrum, Albert Tauler, Ian Pryme, Beate Stern
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Patent number: 9074178Abstract: This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.Type: GrantFiled: May 5, 2014Date of Patent: July 7, 2015Assignee: The California Institute of TechnologyInventors: Frances Arnold, Peter Meinhold, Matthew W. Peters, Rudi Fasan, Mike M. Y. Chen
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Patent number: 9066937Abstract: Epidermal lipoxygenase obtained from axolotl is utilized in pharmaceutical or cosmetic compositions. The compositions have use in wound healing, bone healing or conditioning of injured tissue, e.g. in wound dressings.Type: GrantFiled: May 28, 2010Date of Patent: June 30, 2015Assignee: Medizinische Hochschule HannoverInventors: Peter Vogt, Bjoern Menger, Kerstin Reimers-Fadhlaoui
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Patent number: 9062286Abstract: This invention provides an amadoriase having high substrate specificity to fructosyl valyl histidine. Such amadoriase comprises substitution of one or more amino acid residues at positions corresponding to amino acids selected from the group consisting of position 98, position 259, position 154, position 125, position 261, position 263, position 106, position 103, position 355, position 96, position 66, position 67, position 70, position 100, position 110, position 113, position 114, and position 156 in the amadoriase derived from the genus Coniochaeta. This invention enables accurate measurement of ?-fructosyl valyl histidine derived from the ?-chain amino terminus in glycated hemoglobin in the presence of ?-fructosyl lysine.Type: GrantFiled: August 4, 2011Date of Patent: June 23, 2015Assignee: Kikkoman CorporationInventors: Atsushi Ichiyanagi, Kozo Hirokawa, Yasuko Tanabe, Yosuke Masakari
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Publication number: 20150147783Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymerase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.Type: ApplicationFiled: February 9, 2015Publication date: May 28, 2015Inventors: Steen Troels Joergensen, Jens Toenne Andersen, Niels Banke, Preben Nielsen
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Publication number: 20150147780Abstract: Disclosed herein are embodiments for a novel method of producing an organic compound, including harvesting at least one organic compound from an organism or cell line genetically engineered with a gene for at least one proton-pump protein.Type: ApplicationFiled: November 24, 2014Publication date: May 28, 2015Inventors: Eugene Dinescu, Vincent Dinescu
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Publication number: 20150147451Abstract: Disclosed is a process for extracting an antioxidant cocktail (containing a number of antioxidative molecules including but not limited to superoxide dismutase (SOD), catalase, glutathione, glutathione peroxidase) from animal sources (i.e., blood or liver) so that it can be used as a food and feed preservative in a non-purified state to prevent rancidity. The antioxidant can also be used in other products such as oils, paint, caulking, plastics, or any other product exposed to oxidation. The disclosed antioxidant cocktail can be used in an unpurified state, yet can still function as a potent antioxidant without having to undergo purification via complex, expensive methods such as chromatography or gel filtration.Type: ApplicationFiled: July 16, 2013Publication date: May 28, 2015Inventors: Vladimir Reukov, Alexey Vertegel
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Publication number: 20150147365Abstract: The present invention relates to an oral composition comprising a volatile sulphur compound breath-odour controlling amount of methyl mercaptan oxidase. The oral composition may be used in a method for controlling breath-odour in a host, which method comprises applying the composition to the oral cavity of that host. The invention also provides a polypeptide having methyl mercaptan oxidase activity, wherein said polypeptide is: (a) a polypeptide comprising an amino acid sequence as set out in SEQ ID NO: 2; (b) a polypeptide comprising an amino acid sequence having at least 50% sequence identity with the amino acid sequence of SEQ ID NO: 2; (c) a polypeptide encoded by a polynucleotide comprising the polynucleotide sequence as set out in SEQ ID NO: 1 or SEQ ID NO: 3; or (d) a fragment of a polypeptide as defined in (a), (b) or (c).Type: ApplicationFiled: May 13, 2013Publication date: May 28, 2015Inventors: Luppo Edens, Evert Tjeerd Van Rij, Marco Jan Lambertus De Groot
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Patent number: 9040239Abstract: 5-methylpyrimidine oxygenases and their use in the modification of nucleic acids are described.Type: GrantFiled: March 14, 2013Date of Patent: May 26, 2015Assignee: New England Biolabs, Inc.Inventors: Yu Zheng, Lana Saleh, June Pais, Nan Dai, Richard J. Roberts, Ivan R. Correa, Jr., Megumu Mabuchi, Romualdas Vaisvila
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Patent number: 9040275Abstract: The present invention relates to a method for detoxification of feed products contaminated by the mycotoxin zearalenone.Type: GrantFiled: December 12, 2008Date of Patent: May 26, 2015Assignee: Novozymes A/SInventors: Anders Vikso-Nielsen, Birthe Hauerbach Sorensen
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Patent number: 9040265Abstract: The invention relates to a process for the enantioselective enzymatic reduction of a keto compound to the corresponding chiral hydroxy compound, wherein the keto compound is reduced with an oxidoreductase in the presence of a cofactor, and is characterized in that an oxidoreductase is used which has an amino acid sequence in which (a) at least 70% of the amino acids are identical to the amino acids of one of the amino acid sequences SEQ ID No 1, SEQ ID No 6 and SEQ ID No 8, or (b) at least 55% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 2, or (c) at least 65% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 3, or (d) at least 75% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 4, or (e) at least 65% of the amino acids are identical to the amino acids of the amino acid sequence SEQ ID No 5, or (0 at least 50% of the amino acids are identical to the amino acids of the amino acid sequenceType: GrantFiled: October 25, 2011Date of Patent: May 26, 2015Assignee: IEP GMBHInventors: Anke Tschentscher, Antje Gupta, Maria Bobkova
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Patent number: 9040262Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: GrantFiled: May 4, 2011Date of Patent: May 26, 2015Assignee: Codexis, Inc.Inventors: Michael Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga Modukuru, Emily Mundorff, Derek Smith, Shiwei Song, Wan Lin Yeo
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Publication number: 20150140587Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.Type: ApplicationFiled: July 14, 2014Publication date: May 21, 2015Inventors: Aldis Darzins, Lance Encell, Tonny Johnson, Dieter Klaubert, Georgyi V. Los, Mark McDougall, Keith V. Wood, Monika G. Wood, Chad Zimprich
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Publication number: 20150141617Abstract: The invention features compositions and methods for generation and uses of formylglycine generating enzyme (FGE) variants.Type: ApplicationFiled: September 18, 2014Publication date: May 21, 2015Inventors: Thomas Dierks, Eva Charlotte Ennemann, Karthikeyan Radhakrishnan, Safaraz Alam, Bernhard Schmidt, Michaela Wachs
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Publication number: 20150139977Abstract: The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal ?-1,4-xylosidic linkages or endo-?-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.Type: ApplicationFiled: October 22, 2014Publication date: May 21, 2015Inventors: David Weiner, David Blum, Alexander Varvak, Shaun Healey, Kristine Chang, Geoff Hazlewood, Thomas Todaro, Grace Desantis, Hwai Chang, Connie Jo Hansen, Scott W. Beaver, Thomas Woodward, Charles Hancock
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Patent number: 9034613Abstract: A process for producing a compound which is (3R)-hydroxybutyl (3R)-hydroxybutyrate of formula (I) which process comprises submitting, to enantioselective reduction, a compound of the following formula (II), (III) or (IV).Type: GrantFiled: April 16, 2009Date of Patent: May 19, 2015Assignees: ISIS Innovation Limited, The United States of America, as Represented by the Secretary, Department of Health & Human ServicesInventors: Jeremy Robertson, Kieran Clarke, Richard Lewis Veech
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Publication number: 20150132750Abstract: 5-methylpyrimidine oxygenases and their use in the modification of nucleic acids are described.Type: ApplicationFiled: March 14, 2013Publication date: May 14, 2015Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Yu Zheng, Lana Saleh, June Pais, Nan Dai, Richard J. Roberts, Ivan R. Correa, JR., Megumu Mabuchi, ROMUALDAS VAISVILA
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Patent number: 9029111Abstract: The present invention relates to a polynucleotide encoding an omega 3 (?-3) desaturase from Pythium irregulare with specificity to long chain polyunsaturated omega 6 (?-6) fatty acids as well as a vector containing said polynucleotide, and a host cell containing the vector or the polynucleotide. Moreover, the present invention pertains to a polypeptide encoded by the said polynucleotide, antibodies against the polypeptide as well as a method for the manufacture of the polypeptide. Further, encompassed by the present invention are transgenic non-human organisms. Finally, the present invention relates to methods for the manufacture of compounds and oil- fatty acid- or lipid-containing compositions.Type: GrantFiled: August 16, 2007Date of Patent: May 12, 2015Assignees: BASF Plant Science GmbH, Bioriginal Food & Science Corp.Inventors: Jörg Bauer, Guohai Wu, Xiao Qiu
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Patent number: 9029112Abstract: The present disclosure relates to engineered ketoreductase polypeptides and uses thereof for the preparation of ?-chloroalcohols from ?-chloroketones. Also provided are polynucleotides encoding the engineered ketoreductase polypeptides and host cells capable of expressing the engineered ketoreductase polypeptides.Type: GrantFiled: June 24, 2014Date of Patent: May 12, 2015Assignee: Codexis, Inc.Inventors: Yong Koy Bong, Michael Vogel, Steven J. Collier, Vesna Mitchell, Jagadeesh Mavinahalli
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Publication number: 20150125936Abstract: Provided herein are isolated laccase enzymes and nucleic acids encoding them. Also provided are mediators for laccase reactions. Also provided herein are methods for using laccases to oxidize lignins and other phenolic and aromatic compounds, such as for bio-bleaching and decolorization of wood pulp under high temperature and pH conditions to facilitate a substantial reduction in use of bleaching chemicals, as well as for treatment of fibers.Type: ApplicationFiled: January 7, 2015Publication date: May 7, 2015Inventors: JANNE SAMULI KEROVUO, SYLKE HAREMZA, OLIVER KOCH, TILO HABICHER, DAN E. ROBERTSON, GRACE DESANTIS, RYAN MCCANN, PETER LUGINBUHL
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Publication number: 20150125925Abstract: The present invention provides consumer products comprising a serine protease variant, more specifically subtilisin variants produced there from. Specifically, the present invention provides consumer products comprising a serine protease variant, more specifically a subtilisin variant having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides methods of making such compositions and methods of treating surfaces, particularly fabric surfaces comprising contacting a surface with an aqueous liquor comprising a serine protease variant, more specifically subtilisin variants and an adjunct material.Type: ApplicationFiled: November 5, 2013Publication date: May 7, 2015Applicant: The Procter & Gamble CompanyInventors: Philip Frank SOUTER, Euan John MAGENNIS, Glenn Steven WARD, Neelam S. AMIN, Katherine AUGUSTYN, Joshua R. BASLER, Luis Gustavo CASCAO-PEREIRA, Katherine D. COLLIER, Edward M. CONCAR, David A. ESTELL, James T. KELLIS, JR., Alexander PISARCHIK, Ayrookaran Joseph POULOSE, Jian YAO