Carboxylic Ester Hydrolase (3.1.1) Patents (Class 435/197)
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Patent number: 8859221Abstract: An object to be attained by the present invention is to provide a method for conveniently, rapidly, and specifically measuring HDL-C in a specimen by use of inexpensive materials, and to provide a reagent kit for HDL-C detection and a dry analytical element for HDL-C detection. The present invention provides a method for measuring high density lipoprotein cholesterol (HDL-C) in a body fluid test sample, wherein cholesterol esterase derived from Schizophyllum commune or Pseudomonas sp. and cholesterol oxidase derived from Pseudomonas sp. are used to generate hydrogen peroxide from HDL-C, and thereby HDL-C is selectively measured.Type: GrantFiled: May 10, 2007Date of Patent: October 14, 2014Assignee: FUJIFILM CorporationInventors: Yoshihiko Abe, Nobuhito Masuda, Hiroko Inomata
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Publication number: 20140287477Abstract: Disclosed are compositions and methods relating to variant maltohexaose-forming alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.Type: ApplicationFiled: October 26, 2012Publication date: September 25, 2014Applicant: DANISCO US INC.Inventors: Luis G. Cascao-Pereira, David A. Estell, Marc Kolkman, Harm Mulder
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Patent number: 8841098Abstract: Acetyl xylan esterases and variants thereof having perhydrolytic activity are provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. Multi-component peracid generation systems comprising an enzyme catalyst having perhydrolytic activity are also provided, as are methods of using the present enzyme catalyst to produce peroxycarboxylic acids. The polypeptide having perhydrolytic activity may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, paper pulp processing, and personal care applications.Type: GrantFiled: March 13, 2013Date of Patent: September 23, 2014Assignee: E.I. du Pont de Nemours and CompanyInventors: Mark Scott Payne, Robert DiCosimo
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Patent number: 8834865Abstract: The invention provides methods, compositions, systems, and kits that include an enzyme/substrate co-delivery system. The liquid delivery system includes at least one enzyme encapsulated in a water-soluble polymeric matrix and a substrate for the enzyme in a carrier liquid in which the polymeric matrix is insoluble. When water is added, the polymeric matrix is solubilized and enzyme is released from the matrix, permitting catalytic action upon the substrate.Type: GrantFiled: November 3, 2009Date of Patent: September 16, 2014Assignee: Danisco US Inc.Inventors: Nathaniel T. Becker, Michael Stoner, Mee-Young Yoon
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Publication number: 20140248254Abstract: This invention provides the use of AChE as nuclease. The use of AChE in regulating the stability of nucleic acid and cell apoptosis, as well as a series of agents based on the use, including the agents for promoting or inhibiting cell apoptosis, the agents for inhibiting tumors, and the agents for protecting nucleic acid from impairing, are provided.Type: ApplicationFiled: August 8, 2012Publication date: September 4, 2014Applicant: SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES, CHINESE ACADEMY OF SCIENCESInventors: Xuejun Zhang, Jing Xie, Aiying Du, Kaijie Guo, Jun Wu
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Publication number: 20140248684Abstract: In some aspects, the present invention relates to compositions and methods for the production of human cholinesterases. More particularly, it relates to methods for the production of human cholinesterases using transient expression and vectors for producing the same. In one aspect, the present invention relates to a plant viral vector encoding a plant codon-optimized DNA sequence that results in accumulation of the cholinesterase in a plant leaf at levels greater than 20 mg, and in some embodiments greater than 200 mg, of the enzyme per kilogram of the plant leaf.Type: ApplicationFiled: September 17, 2012Publication date: September 4, 2014Applicant: ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITYInventors: Tsafrir Mor, Latha Kannan, Katherine E. Larrimore
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Patent number: 8815550Abstract: Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.Type: GrantFiled: November 13, 2013Date of Patent: August 26, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Robert DiCosimo, Scott D Cunningham, Stephen R Fahnestock, Tanja Maria Gruber, Mark S Payne, Pierre E Rouviere, Linda Jane Solomon, Hong Wang
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Publication number: 20140234921Abstract: The present invention relates to novel polypeptides, or fragments of polypeptides, genes encoding them and means for producing said polypeptides. In detail the invention relates to polypeptides having esterase, suberinase and/or cutinase activity at low pH. This invention relates also to compositions containing the polypeptides and methods of using the polypeptides.Type: ApplicationFiled: April 11, 2012Publication date: August 21, 2014Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTTInventors: Antti Nyyssola, Hanna Kontkanen, Mari Hakkinen, Ville Pihlajaniemi, Markku Saloheimo, Johanna Buchert, Tiina Nakari-Setala
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Publication number: 20140234915Abstract: The present invention relates enzyme composition comprising a cellulolytic preparation and an acetylxylan esterase (AXE); and the used of cellulolytic enzyme compositions for hydrolyzing acetylated cellulosic material. Finally the invention also relates to processes of producing fermentation products from acetylated cellulosic materials using a cellulolytic enzyme composition of the invention.Type: ApplicationFiled: September 21, 2012Publication date: August 21, 2014Inventors: Prashant Iyer, Armindo Ribiero Gaspar, James Croonenberghs, Thomas P. Binder
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Patent number: 8809030Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: GrantFiled: October 22, 2012Date of Patent: August 19, 2014Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert Dicosimo, Mark Scott Payne, John Edward Gavagan
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Publication number: 20140220658Abstract: Isolated nucleic acids encoding polypeptides that exhibit butyrylcholinesterase (BChE) enzyme activity are disclosed, along with molecular criteria for preparing such nucleic acids, including codon optimization. Methods of preparing modified and/or truncated BChE molecules having selected properties, especially selective formation of monomers, are also described. Vectors and cells containing and/or expressing the nucleic acids are also disclosed.Type: ApplicationFiled: April 3, 2014Publication date: August 7, 2014Applicant: PharmAthene Inc.Inventors: Kalvin Yim, Steven Danso, Edward Hausknecht
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Publication number: 20140209542Abstract: Systems, methods, and compositions for removing organophosphate toxins from blood are disclosed herein. The compositions include a lipid-based capture particle that displays BChE that binds to the toxin. The methods include acoustically separating toxins bound to lipid-based capture particles from blood factors of whole blood.Type: ApplicationFiled: January 30, 2014Publication date: July 31, 2014Applicant: The Charles Stark Draper Laboratory, Inc.Inventors: Tammy Spain, Jason O. Fiering, Leila Albers
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Patent number: 8785169Abstract: The present invention relates to the field of bioengineering and discloses a cutinase-producing genetically engineered microorganism and use thereof. Recombinant plasmid Tfu_0883-hlyAs/pET20b(+) was constructed and transformed into E. coli BL21(DE3) to obtain recombinant E. coli strain Tfu_0883-hlyAs/pET20b(+)/E. coli BL21(DE3). Specific growth rate was maintained at a certain value using fed-batch fermentation mode. After fermenting 30-34 hours, the enzyme activity in the supernatant reached 700-750 U/mL. The present invention uses glycerol as the main raw material and employs semi-synthetic medium, has the advantages of good stability and ease of control, and is suitable for large-scale production.Type: GrantFiled: February 28, 2011Date of Patent: July 22, 2014Assignee: Jiangnan UniversityInventors: Jian Chen, Jing Wu, Dan Wu, Lei Wang
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Publication number: 20140199286Abstract: The present invention provides a process for producing a lyophilized pharmaceutical composition containing a protein. The present invention further provides a product produced by the process. The present invention further provides a process for producing an injectable pharmaceutical composition. The present invention further provides a method of treating a patient with a therapeutic protein composition.Type: ApplicationFiled: January 14, 2014Publication date: July 17, 2014Applicant: TEVA PHARMACEUTICAL INDUSTRIES, LTD.Inventors: Qinghai Zhao, Xia Luo, Jason Bock
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Patent number: 8772007Abstract: The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions for generation of peracids. The present invention finds particular use in applications involving cleaning, bleaching and disinfecting.Type: GrantFiled: December 3, 2004Date of Patent: July 8, 2014Assignee: Danisco US Inc.Inventors: Neelam S. Amin, Matthew G. Boston, Richard R. Bott, Marguerite A. Cervin, Edward M. Concar, Marc E. Gustwiller, Brian E. Jones, Klaus Liebeton, Gregory S. Miracle, Hiroshi Oh, Ayrookaran J. Poulose, Sandra W. Ramer, Jeffrey J. Scheibel, Walter Weyler, Gregory M. Whited
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Publication number: 20140178933Abstract: An expression vector which is capable of overexpressing a protein of interest in a host cell, a host cell comprising the expression vector, and a method of producing a protein of interest are provided.Type: ApplicationFiled: April 30, 2012Publication date: June 26, 2014Applicants: RESEARCH & BUSINESS FOUNDATION SUNGKYUNKWAN UNIVERSITY, Samsung Electronics Co., Ltd.Inventors: Hyun Min Koo, Byung Jo Yu, Ki Sung Lee, Jae Chan Park, Dae Hyuk Kweon, Sung Minm Park
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Patent number: 8753852Abstract: The present invention provides a novel nucleic acid sequence, designated LIP1, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same.Type: GrantFiled: January 12, 2012Date of Patent: June 17, 2014Assignee: Danisco US Inc.Inventors: Brian E. Jones, William D. Grant, Shaun Heaphy, Susan Grant, Helen Rees
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Patent number: 8735125Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: GrantFiled: October 22, 2012Date of Patent: May 27, 2014Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert Dicosimo, Mark Scott Payne, John Edward Gavagan
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Publication number: 20140120586Abstract: The present invention discloses novel polypeptides and enzyme preparations containing them, which improve the efficiency of cellulose and lignocellulose degradation even at elevated temperatures. The polypeptides can be produced using conventional recombinant DNA technologies. The related polynucleotides, vectors and host cells are also disclosed. The polypeptides and the enzyme preparations containing them are particularly useful in improving the efficiency of cellulose and lignocellulose degradation, in improving the quality of animal feed, in machine dishwashing applications, in detergent compositions, in pulp and paper, textile, food, baking or beverage industry.Type: ApplicationFiled: October 23, 2013Publication date: May 1, 2014Applicant: Roal OyInventors: Kristiina JARVINEN, Kari JUNTUNEN, Taija LEINONEN, Alexandra KOMANDER, Kim LANGFELDER, Jari VEHMAANPERA, Terhi PURANEN
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Patent number: 8710156Abstract: The invention relates to functionalized, telechelic polymers synthesized by enzymatic catalysis and methods, and the functionalization of polymers via Michael addition with a lipase catalyst, and the crosslinking of mono- or difunctional (telechelic) polymers made by enzymatic catalysis, such as by using multifunctional coupling agents and enzyme catalysts. Quantitative transesterification of vinyl methacrylate with poly(ethylene glycol), poly(isobutylene) and poly(dimethylsiloxane) was achieved using Candida antarctica lipase B. In addition, methacrylate-functionalized poly(ethylene glycol) monomethyl ether has been successfully coupled to aminoethoxy poly(ethylene glycol) monomethyl ether via Michael addition using Candida antarctica lipase B. Amine-functionalized poly(ethylene glycol)s have also been used for the preparation of poly(ethylene glycol)-based dendrimers and gels through Michael addition of the polymer onto triacryloyl hexahydro-triazine using the same enzyme.Type: GrantFiled: March 26, 2010Date of Patent: April 29, 2014Inventors: Judit E. Puskas, Mustafa Yasin Sen
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Publication number: 20140113966Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: October 22, 2012Publication date: April 24, 2014Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: ROBERT DICOSIMO, MARK SCOTT PAYNE, JOHN EDWARD GAVAGAN
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Patent number: 8703460Abstract: In a method for producing an additive for the enzymatic degradation of mycotoxins, in particular fumonisins, it is provided that at least one nucleic acid sequence of genes corresponding to sequences ID Nos. 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24 is provided, the at least one nucleic acid sequence is expressed in prokaryotic or eukaryotic host cells, and at least one thus prepared enzyme corresponding to sequences ID Nos. 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 and 25, or at least one complete recombinant host organism optionally along with a cosubstrate, are used in a vegetable raw material.Type: GrantFiled: September 18, 2009Date of Patent: April 22, 2014Assignee: Erber AktiengesellschaftInventors: Wulf-Dieter Moll, Doris Hartinger, Karin Grieβler, Eva Maria Binder, Gerd Schatzmayr
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Publication number: 20140100338Abstract: The invention relates to a method for the enzyme-catalysed hydrolysis of polyacrylic acid esters. According to said method, at least one polyacrylic acid ester is provided and incubated with at least one enzyme selected from enzymes (EC 3.1) acting on ester bindings, until the ester groups contained in the polyacrylic acid ester are partially or fully hydrolytically split, and optionally the modified polymer obtained thereby is isolated. The invention also relates to the enzymes used and mutants thereof, nucleic acids coding for the enzymes, vectors comprising the nucleic acids, micro-organisms comprising the vectors, and the use of the enzymes, the vectors or the micro-organisms for carrying out a method for the enzyme-catalysed hydrolysis of polyacrylic acid esters. The present application also relates to polymer reaction products that can be obtained by the method, and methods for producing esterases.Type: ApplicationFiled: December 9, 2013Publication date: April 10, 2014Applicant: BASF SEInventors: Bernhard Hauer, Hans Wolfgang Höffken, Cecilia Kvarnström Branneby, Helmut Schwab, Sabine Feichtenhofer, Marlene Buchebner, Brigitte Pohn
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Publication number: 20140093919Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: July 31, 2012Publication date: April 3, 2014Applicant: Novozymes Inc.Inventor: Nikolaj Spodsberg
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Publication number: 20140093939Abstract: Disclosed is a thermostable tannase derived from a microorganism. Specifically disclosed is a thermostable tannase derived from Aspergillus awamori or Aspergillus niger. A preferred embodiment of the tannase has the following chemoenzymatic properties: (1) activity: to act on a depside bond to thereby cause hydrolysis; (2) molecular weight: about 230,000 Da (as measured by gel filtration); and (3) thermal stability: stable at a temperature up to 65° C. (pH 5.0, 30 min.Type: ApplicationFiled: November 25, 2013Publication date: April 3, 2014Applicant: AMANO ENZYME INC.Inventors: Megumi NAKAGAWA, Naoki MATSUMOTO, Hitoshi AMANO, Shotaro YAMAGUCHI
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Patent number: 8679480Abstract: A method and corresponding pharmaceutical composition to inhibit neuropathic pain by injecting intrathecally into a mammalian subject a neuropathic pain-inhibiting amount of a phosphatase falling within E.C. 3.1.3.x, more specifically a protein phosphatase falling within E.C. 3.1.3.16, and most specifically calcineurin.Type: GrantFiled: June 15, 2012Date of Patent: March 25, 2014Assignee: Wisconsin Alumni Research FoundationInventors: Vjekoslav Miletic, Gordana Miletic
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Publication number: 20140079682Abstract: Isolated polynucleotides and polypeptides encoded therefrom are provided. These include mutated PON enzymes with increased, modified or substantially the same substrate specificity as compared to respective wild-type PON. Also provided are kits and methods using these enzymes.Type: ApplicationFiled: October 21, 2013Publication date: March 20, 2014Applicant: Yeda Research and Development Co., Ltd.Inventors: Dan S. TAWFIK, Amir Aharoni, Leonid Gaydukov, Joel L. Sussman, Israel Silman
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Patent number: 8663616Abstract: Disclosed herein are compositions and methods to treat an oral cavity surface with a peracid-based benefit agent. The peracid benefit agent can be use for oral surface bleaching, whitening, disinfecting, destaining, deodorizing, decreasing or removing biofilm, and combinations thereof. The peracid is enzymatically generated from a carboxylic acid ester substrate using a CE-7 carbohydrate esterase having perhydrolytic activity (perhydrolase) in the presence of a source of peroxygen. A fusion protein comprising the perhydrolase coupled to a peptidic component having affinity for an oral cavity surface, either directly or through an optional linker, may be used to target the perhydrolytic activity to the oral cavity surface.Type: GrantFiled: December 19, 2011Date of Patent: March 4, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Lisa A. Butterick, Scott D. Cunningham, Robert DiCosimo, Kari A. Fosser, Tanja Maria Gruber, Sharon L. Haynie, Mark S. Payne, Pierre E. Rouviere, Hong Wang
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Publication number: 20140051119Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.Type: ApplicationFiled: June 10, 2013Publication date: February 20, 2014Applicant: Codexis, Inc.Inventors: Ryan Fong, Xiyun Zhang, Chirs Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
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Publication number: 20140051146Abstract: In one aspect there is provided a method for producing a recombinant enzyme capable of hydrolysing chlorophyll or a chlorophyll derivative, comprising a step of intracellular expression of the recombinant enzyme in a eukaryotic host cell.Type: ApplicationFiled: February 16, 2012Publication date: February 20, 2014Applicant: DUPONT NUTRITION BIOSCIENCES APSInventor: Susan Mampusti Madrid
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Patent number: 8652455Abstract: Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.Type: GrantFiled: December 19, 2011Date of Patent: February 18, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Robert DiCosimo, Scott D. Cunningham, Stephen R. Fahnestock, Tanja Maria Gruber, Mark S. Payne, Pierre E. Rouviere, Linda Jane Solomon, Hong Wang
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Publication number: 20140045201Abstract: Disclosed is a method for quantifying HDL2 cholesterol in a test sample without requiring laborious operations. The method for quantifying cholesterol comprises allowing phospholipase to act on HDL to quantify cholesterol. Also disclosed is a method comprising: a first step of transferring cholesterols other than high-density lipoproteins in a test sample to the outside of the reaction system; and a second step of quantifying high-density lipoprotein 2 cholesterol among the high-density lipoproteins remaining in the reaction system; wherein, by performing the second step by the above method, high-density lipoprotein 2 cholesterol in the test sample can be quantified.Type: ApplicationFiled: February 27, 2012Publication date: February 13, 2014Applicant: DENKA SEIKEN CO., LTD.Inventors: Maiko Higuchi, Yasuki Itoh
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Publication number: 20140045243Abstract: The present disclosure provides mutant cells for the secretion of proteins and for the degradation of lignocellulosic biomass. Methods for the use of these cells are also provided. Specifically, the utility of combined genetic deletions of ?-glucosidases and the catabolite repressor gene creA/cre-1 for protein secretion in fungal and yeast cells is disclosed.Type: ApplicationFiled: March 15, 2012Publication date: February 13, 2014Applicant: The Regents of the Uniersity of CaliforniaInventors: Elizabeth A. Znameroski, James H. Doudna Cate, N. Louise Glass
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Publication number: 20140045225Abstract: The present invention discloses novel polypeptides and enzyme preparations containing them, which enhance the efficiency of the cellulosic degradation even at elevated temperatures. The polypeptides are produced by recombinant technology, and means for their production are described. The novel polypeptides are useful in processing biomass, and in biofuel, starch, textile, detergent, pulp and paper, food, feed or beverage industries. They may also be used e.g. in cleaning the interior of a dishwashing machine or for biofinishing or biostoning. The novel polypeptides are also useful in animal feed.Type: ApplicationFiled: June 6, 2013Publication date: February 13, 2014Applicant: Roal OyInventors: Susanna MAKINEN, Kari JUNTUNEN, Alexandra KOMANDER, Kim LANGFELDER, Jari VEHMAANPERA, Terhi PURANEN
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Patent number: 8647848Abstract: The invention relates to the enzymatic synthesis of sphingolipids and compositions that contain sphingolipids from lysosphingolipids and carbonic esters, and to cosmetic, dermatological or pharmaceutical formulations containing said sphingolipids or compositions.Type: GrantFiled: May 22, 2009Date of Patent: February 11, 2014Assignee: Evonik Goldschmidt GmbHInventors: Frank Hollmann, Oliver Thum, Christoph Toelle, Angelo Provinzano, Cornelis Gerrit Nijs Korevaar
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Patent number: 8642273Abstract: Disclosed are methods for the preparation and use of labeled AChE and labeled AChE inhibitory conjugate compositions for detecting accumulation of toxic materials such as organophosphates, insecticides, and other nerve agents. Also disclosed are methods for the use of labeled AChE and labeled AChE inhibitory conjugate compositions in a variety of areas, including the detecting of toxic materials in biological samples, in the area of food and water analysis, in environmental monitoring, and in industrial settings.Type: GrantFiled: April 16, 2003Date of Patent: February 4, 2014Assignee: The Regents of the University of CaliforniaInventors: Palmer Taylor, Zoran Radic, Jianxin Shi, Aileen Boyd
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Patent number: 8637290Abstract: The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, phosphatidic acid phosphatases (PAP) and/or lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided.Type: GrantFiled: September 21, 2007Date of Patent: January 28, 2014Assignee: DSM IP Assets B.V.Inventors: Eileen O'Donoghue, Nelson R. Barton
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Patent number: 8637101Abstract: The present invention relates to novel polynucleotide sequences comprising genes that encode novel lipolytic enzymes, as well as functional equivalents of the gene or the amino acid sequences with high homology thereto. The invention also relates to methods of using these lipolytic enzymes in industrial processes, for example in the dairy or baking industry.Type: GrantFiled: February 26, 2009Date of Patent: January 28, 2014Assignee: DSM IP Assets B.V.Inventors: Jan Metske Laan Van Der, Yulia M. Efimova, Karin Turk, Margot Elisabeth Francoise Schooneveld-Bergmans, Arie Gerrit Terdu, Arjen Sein, Natalja Alekseevna Cyplenkova
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Patent number: 8633012Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.Type: GrantFiled: October 11, 2012Date of Patent: January 21, 2014Assignee: Solazyme, Inc.Inventors: Scott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walt Rakitsky, Xinhua Zhao, Riyaz Bhat
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Publication number: 20130337277Abstract: A liquid composition for producing a self-destructible temporary structure by additive manufacturing is provided. The liquid composition includes a biosynthetic copolymer as a degradable component and an enzyme as a disintegrating agent, the disintegrating agent is capable of disintegrating the degradable component, wherein after exposure to an external trigger, the disintegrating agent gradually degrades the degradable component.Type: ApplicationFiled: June 25, 2013Publication date: December 19, 2013Inventors: Daniel DIKOVSKY, Eduardo Napadensky
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Publication number: 20130330781Abstract: The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity, which perform very well in industrial applications such as laundry compositions, for biopolishing of newly manufactured textiles, for providing an abraded look of cellulosic fabric or garment, and for treatment of paper pulp. Further, the invention relates to DNA constructs encoding such enzymes, a method for providing a gene encoding for such enzymes, a method of producing the enzymes, enzyme preparations containing such enzymes, and the use of these enzymes for a number of industrial applications.Type: ApplicationFiled: November 21, 2012Publication date: December 12, 2013Applicant: NOVOZYMES A/SInventors: Martin Schulein, Lene Nonboe Andersen, Soren Flensted Lassen, Markus Sakari Kauppinen, Lene Lange, Ruby Ilum Nielsen, Shinobu Takagi, Michiko Ihara
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Publication number: 20130330804Abstract: In one aspect, there is provided a process for treating a plant oil, comprising a step of contacting the oil with an enzyme, wherein the enzyme is capable of hydrolysing an a? or b? stereoisomer of chlorophyll or a chlorophyll derivative.Type: ApplicationFiled: February 16, 2012Publication date: December 12, 2013Applicant: DUPONT NUTRITION BIOSCIENCES, APSInventors: Jorn Borch Soe, Tina Lillan Jorgensen, Lene Lauridsen, Rene Mikkelsen, Janne Brunstedt
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Patent number: 8597927Abstract: The invention provides methods, compositions, and kits for removal of biofilms from surfaces. The methods described herein comprise simultaneous or sequential application of a perhydrolase enzyme and a mixture of other enzymes, such as proteases, glucanases, esterases, mannanases, phospholipases, cellulases, and/or amylases, to a biofilm on a surface, to effect removal of the biofilm.Type: GrantFiled: December 16, 2008Date of Patent: December 3, 2013Assignee: Danisco US Inc.Inventors: Christopher C. Barnett, Manoj Kumar, Gregory M. Whited
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Publication number: 20130316406Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.Type: ApplicationFiled: June 28, 2013Publication date: November 28, 2013Applicant: Battelle Energy Alliance, LLCInventors: David N. Thompson, William A. Apel, Vicki S. Thompson, David W. Reed, Jeffrey A. Lacey, Emily D. Henriksen
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Publication number: 20130315915Abstract: Methods and compositions are provided for assessing, treating, and preventing diseases, especially cancer, using cancer-associated targets (CAT). Methods and compositions are also provided for determining or predicting the effectiveness of a treatment for these diseases or for selecting a treatment, using CAT. Methods and compositions are further provided for modulating cell function using CAT. Also provided are compositions that modulate CAT (e.g., antagonists or agonists), such as antibodies, proteins, small molecule compounds, and nucleic acid agents (e.g., RNAi and antisense agents), as well as pharmaceutical compositions thereof. Further provided are methods of screening for agents that modulate CAT, and agents identified by these screening methods.Type: ApplicationFiled: April 30, 2013Publication date: November 28, 2013Applicant: Celera CorporationInventors: Steve RUBEN, Tao HE, Candy LEE, Karen VAN ORDEN, Paul MOORE
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Patent number: 8592193Abstract: Butyrylcholinesterase (BChE) polypeptide variants of the presently-disclosed subject matter have enhanced catalytic efficiency for (?)-cocaine, as compared to wild-type BChE. Pharmaceutical compositions of the presently-disclosed subject matter include a BChE polypeptide variant having an enhanced catalytic efficiency for (?)-cocaine. A method of the presently-disclosed subject matter for treating a cocaine-induced condition includes administering to an individual an effective amount of a BChE polypeptide variant, as disclosed herein, to lower blood cocaine concentration.Type: GrantFiled: February 17, 2012Date of Patent: November 26, 2013Assignee: University of Kentucky Research FoundationInventors: Chang-Guo Zhan, Fang Zheng, Wenchao Yang, Liu Xue, Shurong Hou
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Publication number: 20130309726Abstract: The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: ApplicationFiled: June 30, 2011Publication date: November 21, 2013Applicant: DUPONT NUTRITION BIOSCIENCES APSInventors: Peter Edvard Degn, Richard R. Bott, Casper Willem Vroemen, Martijn Silvan Scheffers, Wolfgang Aehle, Elin Petersen
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Patent number: 8580549Abstract: The invention relates to agents containing esterases, and to the use thereof for dressing fibres, in particular, artificial fibres, washing and cleaning agents comprising esterases and corresponding washing and cleaning methods, in addition to additional technical areas of application. The invention also relates to the use of esterases for protecting against or reducing and/or preventing pilling, preferably in textiles, particularly plastic fibres, more preferably polyester fibres, in addition to the use of esterases for separating the plastics, in particular, polyester compounds. The invention further relates to novel esterases and to sufficiently related proteins and to derivatives thereof, agents containing them and to the use thereof.Type: GrantFiled: August 3, 2006Date of Patent: November 12, 2013Assignee: Henkel KGaAInventors: Andreas Michels, André Pütz, Karl-Heinz Maurer, Thorsten Eggert, Karl-Erich Jäger
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Patent number: 8580538Abstract: Ethylenically unsaturated glycosides of the formula I: wherein n, A, X, R3 and R4 have the meanings given in the description are produced by reacting an ethylenically unsaturated alcohol of formula II: with a saccharide of formula III: in the presence of a glucosidase at a molar ratio of the alcohol to the saccharide of from 2:1 to 30:1 in the presence of a solvent mixture of water and 1,4-dioxane at a weight ratio of 0.1:1 to 9:1 and a weight ratio of solvent mixture to saccharide of from 3:1 to 30:1.Type: GrantFiled: July 28, 2011Date of Patent: November 12, 2013Assignee: BASF SEInventors: Harald Keller, Katja Loos, Wouter Kloosterman
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Publication number: 20130288316Abstract: Acetyl xylan esterases and variants thereof having perhydrolytic activity are provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. Multi-component peracid generation systems comprising an enzyme catalyst having perhydrolytic activity are also provided, as are methods of using the present enzyme catalyst to produce peroxycarboxylic acids. The polypeptide having perhydrolytic activity may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, paper pulp processing, and personal care applications.Type: ApplicationFiled: March 13, 2013Publication date: October 31, 2013Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventor: E. I. DU PONT DE NEMOURS AND COMPANY