Carboxylic Ester Hydrolase (3.1.1) Patents (Class 435/197)
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Publication number: 20110236337Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: March 23, 2011Publication date: September 29, 2011Inventors: Robert Dicosimo, John Edward Gavagan, Mark Scott Payne
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Publication number: 20110236335Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: March 23, 2011Publication date: September 29, 2011Inventors: Robert Dicosimo, John Edward Gavagan, Mark Scott Payne
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Publication number: 20110236336Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: March 23, 2011Publication date: September 29, 2011Inventors: ROBERT DICOSIMO, John Edward Gavagan, Mark Scott Payne
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Publication number: 20110236339Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: March 23, 2011Publication date: September 29, 2011Inventors: ROBERT DICOSIMO, John Edward Gavagan, Mark Scott Payne
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Publication number: 20110212504Abstract: The present application provides a method of producing a carboxylesterase or its variant in eukaryotic cells. The present application also provides an expression vector for high level carboxylesterase expression, a eukaryotic cell comprising the expression vector, and uses thereof. The present application also provides a composition comprising a carboxylesterase or its variant produced by a method described in the present application and uses of the composition.Type: ApplicationFiled: June 2, 2010Publication date: September 1, 2011Applicant: TONGJI UNIVERSITYInventor: Zhongbin Liu
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Patent number: 8008051Abstract: The invention relates to novel proteins from Pseudomonas glumae, having esterase activity, in particular butynol I esterase activity, to nucleic acid sequences coding therefore, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.Type: GrantFiled: April 13, 2009Date of Patent: August 30, 2011Assignee: BASF AktiengesellschaftInventors: Bernhard Hauer, Thomas Friedrich, Christoph Nübling, Rainer Stürmer, Wolfgang Ladner
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Patent number: 8003361Abstract: Methods for preferentially hydrolyzing one stereoisomer of an isoxazoline diester over another, as well as an enzyme for facilitating the preferential hydrolysis are provided. Also provided are methods for providing mixtures of (RR) and (RS) monatin as well as (SS) and (SR) monatin, which methods can include the step of stereoselectively hydrolyzing an isoxazoline diester.Type: GrantFiled: October 1, 2007Date of Patent: August 23, 2011Assignee: Cargill IncorporatedInventors: Dean Brady, Lucia H. Steenkamp, Amanda Louise Rousseau, Subash Buddoo, Paul A. Steenkamp
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Publication number: 20110201069Abstract: An object of the present invention is to provide a method for efficiently producing an oligomer or a monomer by degrading a biodegradable resin using an enzyme, so that the oligomer or the monomer can be recovered. The present invention provides a method for producing an oligomer and/or a monomer by degrading a biodegradable resin in a degradation liquid containing a biodegradation enzyme, a buffer agent, an organic solvent, and water. In this method, the SP value of the organic solvent is less than 8.5 or more than 11.5, and the percentage content of the organic solvent (by volume) in the degradation liquid is higher than 1% and lower than 15%. In the method for producing an oligomer or a monomer, the degradation percentage of the biodegradable resin is low, and deposits of aggregates of the oligomer and/or the monomer are few, so that the recovery percentage is high.Type: ApplicationFiled: October 27, 2009Publication date: August 18, 2011Applicant: TOYO SEIKAN KAISHA LTD.Inventors: Seishi Yoshikawa, Tsutaki Katayama, Masahito Kogure
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Publication number: 20110201082Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: April 25, 2011Publication date: August 18, 2011Applicant: Novozymes A/SInventors: Katja Salomon Johansen, Keith Gibson, Preben Nielsen, Helle Outtrup
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Publication number: 20110195155Abstract: Disclosed is a thermostable tannase derived from a microorganism. Specifically disclosed is a thermostable tannase derived from Aspergillus awamori or Aspergillus niger. A preferred embodiment of the tannase has the following chemoenzymatic properties: (1) activity: to act on a depside bond to thereby cause hydrolysis; (2) molecular weight: about 230,000 Da (as measured by gel filtration); and (3) thermal stability: stable at a temperature up to 65° C. (pH 5.0, 30 min.Type: ApplicationFiled: October 2, 2009Publication date: August 11, 2011Applicant: AMANO ENZYME INC.Inventors: Megumi Nakagawa, Naoki Matsumoto, Hitoshi Amano, Shotaro Yamaguchi
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Publication number: 20110195478Abstract: The present invention discloses a bladder cancer biomarker and a test method using the same. The biomarker contains at least one of the mentioned 69 compounds, such as apolipoprotein A1 (APOA1), apolipoprotein A2 (APOA2), peroxiredoxin 2 (PRDX2), heparin cofactor 2 precursor (HCII), and serum amyloid A-4 protein (SAA4), which exist in the urine specimen of a testee. The expression intensity of the biomarker can facilitate diagnosis of bladder cancer and evaluation of aggressiveness and malignancy of bladder cancer. Thereby, the physician can arrange an optimized treatment to achieve the best therapeutic effect.Type: ApplicationFiled: August 4, 2010Publication date: August 11, 2011Inventors: Yi-Ting Chen, Jau-Song Yu, Chien-Lun Chen, Yu-Sun Chang
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Publication number: 20110189755Abstract: In a method for producing an additive for the enzymatic degradation of mycotoxins, in particular fumonisins, it is provided that at least one nucleic acid sequence of genes corresponding to sequences ID Nos. 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24 is provided, the at least one nucleic acid sequence is expressed in prokaryotic or eukaryotic host cells, and at least one thus prepared enzyme corresponding to sequences ID Nos. 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23 and 25, or at least one complete recombinant host organism optionally along with a cosubstrate, are used in a vegetable raw material.Type: ApplicationFiled: September 18, 2009Publication date: August 4, 2011Inventors: Wulf-Dieter Moll, Doris Hartinger, Karin Grießler, Eva Maria Binder, Gred Schtzmayr
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Patent number: 7981643Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: GrantFiled: February 28, 2011Date of Patent: July 19, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert Dicosimo, John Edward Gavagan, Mark Payne
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Patent number: 7981650Abstract: The invention relates to the use of fusion proteins between at least two plant cell-wall degrading enzymes, the enzymes being such that they do not contain a C-terminal carbohydrate-binding-molecule (CBM), and optionally a CBM, the enzymes and CBM being recombinant proteins corresponding to native proteins in fungi, or mutated forms thereof, for carrying out processes of plant cell-wall degradation in the frame of the preparation, from plants or vegetal by-products, of compounds of interest located in plant cell-wall, or in the frame of the bleaching of pulp and paper.Type: GrantFiled: July 26, 2006Date of Patent: July 19, 2011Assignees: Institut National de la Recherche Agronomique, Institut Francais du PetroleInventors: Anthony Levasseur, David Navarro, Peter Punt, Jean-Pierre Belaïch, Marcel Asther, Frédéric Monot, Eric Record
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Patent number: 7981645Abstract: A process for the stereoselective decarboxylation of malonic acid derivatives with mutated decarboxylases is disclosed.Type: GrantFiled: February 4, 2005Date of Patent: July 19, 2011Assignee: BioSpring GmbHInventors: Hüseyin Aygün, Sylvia Wojczewski, Markus Kircher, Susann Rosmus
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Patent number: 7981644Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: GrantFiled: February 28, 2011Date of Patent: July 19, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert Dicosimo, John Edward Gavagan, Mark S. Payne
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Patent number: 7977080Abstract: The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, phosphatidic acid phosphatases (PAP)) and/or lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided.Type: GrantFiled: March 8, 2005Date of Patent: July 12, 2011Assignee: Verenium CorporationInventors: Svetlana Gramatikova, Geoff Hazlewood, David Lam, Nelson R. Barton, Blake G. Sturgis, Dan E. Robertson, Jincai Li, Joel A. Kreps, Roderick James Fielding, Robert C. Brown, Amit Vasavada, Xuqiu Tan, Adrian Badillo, Wilhelmus P. Van Hoek, Giselle Janssen, Charles Isaac, Mark J. Burk
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Publication number: 20110165141Abstract: Polynucleotides encoding a mutant human carboxylesterase enzyme and polypeptides encoded by the polynucleotides which are capable of metabolizing a prodrug and inactive metabolites thereof to active drug are provided. Compositions and methods for sensitizing cells to a prodrug agent, inhibiting cell growth, treating drug addiction, and facilitating the metabolism of an organophosphate with this enzyme are also provided. In addition, a screening assay for identification of drugs activated by this enzyme is described.Type: ApplicationFiled: February 4, 2011Publication date: July 7, 2011Applicants: St. Jude Children's Research Hospital, The University of North Carolina at Chapel HillInventors: Philip M. Potter, Monika Weirdl, Matthew R. Redinbo
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Patent number: 7972831Abstract: The inventors have identified polypeptides having lipase activity in anaerobic thermophilic bacteria. Accordingly, the invention provides a process for hydrolyzing an ester bond in a substrate, which comprises treating the substrate with a particular lipase (a polypeptide having lipase activity). The invention also provides a lipase for use in the process and a polynucleotide encoding the lipase.Type: GrantFiled: October 5, 2005Date of Patent: July 5, 2011Assignee: Novozymes A/SInventors: Thomas Schafer, Garabed Antranikian, Maryna Royter, Tine Hoff
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Publication number: 20110151540Abstract: A process for preparing a phospholipase D of vegetable or nut origin comprises: (i) providing a liquid extract of vegetable or nut origin; (ii) combining the liquid extract with a precipitating agent comprising at least one surfactant optionally in admixture with phospholipids, mono-, di- or triglycerides or a mixture thereof, to produce a suspension comprising a supernatant and a precipitate; and (iii) separating and collecting the precipitate from the suspension produced in (ii).Type: ApplicationFiled: May 8, 2009Publication date: June 23, 2011Inventors: Victoria Taran, Yuri Fedorovich Drygin, Vera Sergeevna Zueva, Raisa Anvarovna Galiullina
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Publication number: 20110152369Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: February 28, 2011Publication date: June 23, 2011Inventors: ROBERT DICOSIMO, John Edward Gavagan, Mark S. Payne
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Publication number: 20110152370Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: February 28, 2011Publication date: June 23, 2011Inventors: ROBERT DICOSIMO, John Edward Gavagan, Mark S. Payne
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Publication number: 20110150857Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: February 28, 2011Publication date: June 23, 2011Inventors: Robert Dicosimo, John Edward Gavagan, Mark S. Payne
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Publication number: 20110152368Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: February 28, 2011Publication date: June 23, 2011Inventors: ROBERT DICOSIMO, John Edward Gavagan, Mark S. Payne
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Patent number: 7964378Abstract: A method is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity derived from Bacillus sp. to produce peroxycarboxylic acids.Type: GrantFiled: December 12, 2006Date of Patent: June 21, 2011Assignee: E.I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, John E. Gavagan, Mark S. Payne, Frederick B. Cooling, III
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Patent number: 7960528Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: GrantFiled: December 7, 2009Date of Patent: June 14, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, John Edward Gavagan, Mark S. Payne
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Patent number: 7960151Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: GrantFiled: February 28, 2011Date of Patent: June 14, 2011Assignee: E.I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, John Edward Gavagan, Mark S. Payne
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Publication number: 20110136908Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritime acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: January 31, 2011Publication date: June 9, 2011Inventors: Robert DiCosimo, John Edward Gavagan, Mark S. Payne
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Publication number: 20110136137Abstract: The invention is directed to biomarkers for determining the EGFR kinase activity in a subject, and the use thereof for predicting and monitoring therapeutic intervention in cancer patients. Areas of application are the life sciences: biology, biochemistry, biotechnology, medicine and medical technology. The biomarkers are selected from a first group consisting of Amy 1, Apo Al, Carbx, Casp, AFP, ApoM, SAP, Fib-a, Fib-b, Fib-g, ApoE, A2MG, A2MG isoform, Serpin, Clusterin, MHC-fB, SAP isoform, or from a second group consisting of Gpx3, properidin, MUP1, HMW-K, Lifr-p, Orm 1, MBL-A, MBP-C, wherein the biomarkers are regulated by EGF overexpression in a subject.Type: ApplicationFiled: December 11, 2008Publication date: June 9, 2011Inventors: Jurgen Borlak, Guiseppe Gazzana
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Publication number: 20110137002Abstract: The invention relates to a method for the enzyme-catalysed hydrolysis of polyacrylic acid esters. According to said method, at least one polyacrylic acid ester is provided and incubated with at least one enzyme selected from enzymes (EC 3.1) acting on ester bindings, until the ester groups contained in the polyacrylic acid ester are partially or fully hydrolytically split, and optionally the modified polymer obtained thereby is isolated. The invention also relates to the enzymes used and mutants thereof, nucleic acids coding for the enzymes, vectors comprising the nucleic acids, micro-organisms comprising the vectors, and the use of the enzymes, the vectors or the micro-organisms for carrying out a method for the enzyme-catalysed hydrolysis of polyacrylic acid esters. The present application also relates to polymer reaction products that can be obtained by the method, and methods for producing esterases.Type: ApplicationFiled: July 17, 2009Publication date: June 9, 2011Applicant: BASF SEInventors: Bernhard Hauer, Hans Wolfgang Höffken, Cecilia Kvarnström Branneby, Helmut Schwab, Sabine Feichtenhofer, Marlene Buchebner, Brigitte Pohn
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Patent number: 7951567Abstract: A method is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity derived from Bacillus sp. to produce peroxycarboxylic acids.Type: GrantFiled: January 22, 2010Date of Patent: May 31, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, John Edward Gavagan, Mark Scott Payne, Frederick B. Cooling, III
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Patent number: 7951566Abstract: A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided.Type: GrantFiled: May 2, 2007Date of Patent: May 31, 2011Assignee: E.I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, John E. Gavagan, Mark S. Payne, Frederick B. Cooling, III
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Patent number: 7943336Abstract: The present invention relates to a method comprising treatment with cutinase for detoxification of feed products contaminated by the mycotoxin zearalenone.Type: GrantFiled: December 19, 2008Date of Patent: May 17, 2011Assignee: Novozymes A/SInventors: Anders Viksoe-Nielsen, Birthe Hauerbach Soerensen
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Patent number: 7943360Abstract: The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided.Type: GrantFiled: January 22, 2007Date of Patent: May 17, 2011Assignee: Verenium CorporationInventors: Svetlana Gramatikova, Geoff Hazlewood, David Lam, Nelson R. Barton, Blake G. Sturgis, Dan E. Robertson, Jincai Li, Joel A. Kreps, Roderick James Fielding, Robert C. Brown, Amit Vasavada, Xuqiu Tan, Adrian Badillo, Wilhelmus P. Van Hoek, Giselle Janssen, Charles Isaac, Mark J. Burk
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Patent number: 7935515Abstract: Disclosed herein are obligate heterotrophic microalgae cells containing an exogenous gene. In some embodiments the gene is a sucrose utilization gene, and further disclosed are methods of manufacturing triglyceride oils using sugar cane or sugar beets as a feedstock in a heterotrophic fermentation. In other embodiments the feedstock is depolymerized cellulosic material. Also disclosed are cells that produce medium chain fatty acids at levels not produced in non-recombinant cells of the same species and genus.Type: GrantFiled: November 30, 2009Date of Patent: May 3, 2011Assignee: Solazyme, Inc.Inventors: Scott Franklin, Aravind Somanchi, Karen Espina, George Rudenko, Penelope Chua
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Patent number: 7932072Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: GrantFiled: December 7, 2009Date of Patent: April 26, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, John Edward Gavagan, Mark S. Payne
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Publication number: 20110091950Abstract: The present invention relates to isolated polypeptides having alpha-glucuronidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 3, 2010Publication date: April 21, 2011Applicant: Novozymes A/SInventors: Eva H. Hansen, Dominique A. Skovland, Hanne R. Soerensen
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Patent number: 7927854Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: GrantFiled: December 7, 2009Date of Patent: April 19, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, John Edward Gavagan, Mark S. Payne
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Publication number: 20110086408Abstract: Described are compositions and methods relating to cellulase/hemicellulase enzyme blends for improving the enzymatic hydrolysis of cellulosic and hemicellulosic materials, as commonly found in biomassType: ApplicationFiled: March 20, 2009Publication date: April 14, 2011Applicant: Danisco US Inc.Inventors: Scott D. Power, Robert M. Caldwell, Suzanne E. Lantz, Edmund A. Larenas
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Patent number: 7923233Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: GrantFiled: December 7, 2009Date of Patent: April 12, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert Dicosimo, John Edward Gavagan, Mark S. Payne
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Publication number: 20110081454Abstract: The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved stability in the presence of a metal chelator. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications comprising citrate. In some particularly preferred embodiments, the present invention provides methods and compositions comprising variant neutral metalloprotease(s) engineered to resist citrate-induced autolysis.Type: ApplicationFiled: October 23, 2008Publication date: April 7, 2011Inventors: Ronaldus W. J. Hommes, Amy D. Liu, Andrew Shaw, Louise Wallace
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Patent number: 7919082Abstract: A novel computational method and generation of mutant butyrylcholinesterase for cocaine hydrolysis is provided. The method includes molecular modeling a possible BChE mutant and conducting molecular dynamics simulations and hybrid quantum mechanical/molecular mechanical calculations thereby providing a screening method of possible BChE mutants by predicting which mutant will lead to a more stable transition state for a rate determining step. Site-directed mutagenesis, protein expression, and protein activity is conducted for mutants determined computationally as being good candidates for possible BChE mutants, i.e., ones predicted to have higher catalytic efficiency as compared with wild-type BChE. In addition, mutants A199S/A328W/Y332G, A199S/F227A/A328W/Y332G, A199S/S287G/A328W/Y332G, A199S/F227A/S287G/A328W/Y332G, and A199S/F227A/S287G/A328W/E441D all have enhanced catalytic efficiency for (?)-cocaine compared with wild-type BChE.Type: GrantFiled: April 1, 2010Date of Patent: April 5, 2011Assignee: University of Kentucky Research FoundationInventors: Chang-Guo Zhan, Hoon Cho, Hsin-Hsiung Tai
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Patent number: 7910347Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: GrantFiled: December 7, 2009Date of Patent: March 22, 2011Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert DiCosimo, John Edward Gavagan, Mark S. Payne
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Publication number: 20110064716Abstract: A method of treating or preventing organophosphate exposure associated damage in a subject in need thereof is provided. The method comprising providing the subject with a therapeutically effective amount of AChE-R to thereby treat the organophosphate exposure associated damage in the subject.Type: ApplicationFiled: August 20, 2007Publication date: March 17, 2011Applicant: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.Inventors: Hermona Soreq, Tsafrir Mor
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Patent number: 7906596Abstract: Processes for enzymatic synthesis comprising: (a) providing an aliphatic poly(oxyalkylene)amine; and (b) reacting the aliphatic poly(oxyalkylene)amine with a reactant selected from the group consisting of acrylic acid compounds and alkyl esters thereof, in the presence of a hydrolase in bulk or in a liquid reaction medium comprising an organic solvent to for a poly(oxyalkylene)acrylamide.Type: GrantFiled: April 20, 2006Date of Patent: March 15, 2011Assignee: BASF SEInventors: Dietmar Häring, Bernhard Hauer, Stefan Becker
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Patent number: 7892537Abstract: Butyrylcholinesterase (BChE) polypeptide variants of the presently-disclosed subject matter have enhanced catalytic efficiency for (?)-cocaine, as compared to wild-type BChE. Pharmaceutical compositions of the presently-disclosed subject matter include a BChE polypeptide variant having an enhanced catalytic efficiency for (?)-cocaine. A method of the presently-disclosed subject matter for treating a cocaine-induced condition includes administering to an individual an effective amount of a BChE polypeptide variant, as disclosed herein, to lower blood cocaine concentration.Type: GrantFiled: April 26, 2010Date of Patent: February 22, 2011Assignee: University of Kentucky Research FoundationInventors: Chang-Guo Zhan, Fang Zheng, Wenchao Yang
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Patent number: 7883882Abstract: Disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Methods such as transesterification, hydrogenation, hydrocracking, deoxygenation, isomerization, interesterification, hydroxylation, hydrolysis and saponification are disclosed. Novel oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods of the invention.Type: GrantFiled: November 30, 2009Date of Patent: February 8, 2011Assignee: Solazyme, Inc.Inventors: Scott Franklin, Aravind Somanchi, Karen Espina, George Rudenko, Penelope Chua
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Patent number: 7879587Abstract: Variants with increased acyl transferase activity can be designed on the basis of a three-dimensional model by making amino acid alterations near the active Ser of lipolytic enzymes such as C. antarctica lipase A or the lipase/acyl transferase from C. parapsilosis.Type: GrantFiled: October 2, 2007Date of Patent: February 1, 2011Assignee: Novozymes A/SInventors: Allan Svendsen, Jesper Vind, Shamkant Anant Patkar, Kim Borch
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Publication number: 20110008795Abstract: Disclosed is a polynucleotide marker or a protein marker for use in the specific detection of an IL-17-producing helper T-cell (a Th17 cell). Also disclosed is a method for detecting a Th17 cell, which is characterized by detecting the occurrence of the polynucleotide marker or the protein marker.Type: ApplicationFiled: February 27, 2009Publication date: January 13, 2011Applicant: SYSMEX CORPORATIONInventors: Masafumi Ikeda, Hitoshi Uga, Satoshi Tanaka, Yoshiaki Miyamoto, Masatoshi Yanagida, Hirokazu Kurata, Masakazu Kadowaki
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Publication number: 20100330647Abstract: The present invention provides methods and compositions comprising at least one perhydrolase enzyme for cleaning and other applications. In some embodiments, the present invention provides methods and compositions for generation of long chain peracids. Certain embodiments of the present invention find particular use in applications involving cleaning, bleaching and disinfecting.Type: ApplicationFiled: May 21, 2010Publication date: December 30, 2010Inventors: NEELAM S. AMIN, RICHARD R. BOTT, MARGUERITE A. CERVIN, AYROOKARAN J. POULOSE, WALTER WEYLER