Acting On Glycosyl Compound (3.2) Patents (Class 435/200)
  • Publication number: 20140315273
    Abstract: A ?-mannanase having increased enzymaic activity is disclosed. The ?-mannanase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of Tyrosine at position 216 with Tryptophan.
    Type: Application
    Filed: September 5, 2013
    Publication date: October 23, 2014
    Applicant: Dongguan APAC Biotechnology CO., Ltd.
    Inventors: Rey-Ting Guo, Jian-Wen Huang, Ya-Shan Cheng, Tzu-Hui Wu, Hui-Lin Lai, Cheng-Yen Lin, Ting-Yung Huang
  • Patent number: 8865447
    Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: October 21, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8865446
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: November 14, 2013
    Date of Patent: October 21, 2014
    Assignees: Novozymes A/S, Novozymes, Inc.
    Inventors: Lan Tang, Ye Liu, Junxin Duan, Wenping Wu, Randall Kramer
  • Patent number: 8865444
    Abstract: A ?-mannanase having increased enzymaic activity is disclosed. The ?-mannanase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of Tyrosine at position 216 with Tryptophan.
    Type: Grant
    Filed: September 5, 2013
    Date of Patent: October 21, 2014
    Assignee: Dongguan APAC Biotechnology Co., Ltd.
    Inventors: Rey-Ting Guo, Jian-Wen Huang, Ya-Shan Cheng, Tzu-Hui Wu, Hui-Lin Lai, Cheng-Yen Lin, Ting-Yung Huang
  • Patent number: 8865445
    Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: October 11, 2013
    Date of Patent: October 21, 2014
    Assignee: Novozymes, Inc.
    Inventors: Suchindra Maiyuran, Randall Kramer, Paul Harris
  • Patent number: 8859254
    Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: December 12, 2013
    Date of Patent: October 14, 2014
    Assignees: Novozymes, Inc., Novozymes A/S
    Inventor: Marc Dominique Morant
  • Patent number: 8859253
    Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: September 23, 2013
    Date of Patent: October 14, 2014
    Assignee: Novozymes, Inc.
    Inventor: Mark Wogulis
  • Patent number: 8859227
    Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: August 1, 2012
    Date of Patent: October 14, 2014
    Assignee: Novozymes, Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8846364
    Abstract: The present invention relates to protein engineering, and concerns especially family G/11 xylanases, and genes encoding said enzymes. In specific, the invention concerns Trichoderma reesei XYNII gene, which codes for endo-1,4-?-xylanase (EC 3.2.1.8). The invention describes how site-directed mutagenesis can be used to improve the properties of an enzyme to match the industrial conditions where it is used. Protein engineering can be used to improve thermoactivity and thermostability of xylanases, as well as to broaden their pH range.
    Type: Grant
    Filed: March 22, 2013
    Date of Patent: September 30, 2014
    Assignee: Danisco US Inc.
    Inventors: Fred Fenel, Ossi Turunen, Matti Leisola
  • Publication number: 20140287465
    Abstract: The present invention relates to polypeptides having xylanase activity, catalytic domains, and carbohydrate binding domains, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding domains. The present invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding domains.
    Type: Application
    Filed: December 18, 2012
    Publication date: September 25, 2014
    Inventors: Nikolaj Spodsberg, Tarana Shaghasi
  • Publication number: 20140256018
    Abstract: The invention relates to recombinantly produced ?-glucosidase variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant ?-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target ?-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.
    Type: Application
    Filed: May 28, 2014
    Publication date: September 11, 2014
    Applicant: Codexis, Inc.
    Inventors: Xiyun Zhang, Jie Yang, Dipnath Baidyaroy, Sally R. Postlethwaite, Attila Andor, Louis A. Clark, Lisa Marie Newman
  • Patent number: 8821862
    Abstract: The present invention provides compositions comprising an antibiofilm enzyme, a soluble ?-N-acetylglucosaminidase similar to the dspB gene (DispersinB®), and an antimicrobial for preventing growth and proliferation of biofilm-embedded microorganisms in acute and chronic wounds, and methods of treatment. The invention further provides methods for preparing medical devices, and in particular, wound care devices using soluble ?-N-acetylglucosaminidase based antimicrobial compositions.
    Type: Grant
    Filed: March 9, 2012
    Date of Patent: September 2, 2014
    Assignees: Kane Biotech Inc., University of Medicine and Dentistry of New Jersey
    Inventors: Srinivasa Madhyastha, Nanda Yakandawala, Purushottam V. Gawande, Karen Lovetri, Jeffrey B. Kaplan, Daniel Rhoads, Lasha Gogokhia
  • Patent number: 8815558
    Abstract: The invention relates to the discovery of novel Chondroitinase Glycoproteins (CHASEGPs), methods of manufacture, and potential uses in conditions where removal of chondroitin sulfates may be of therapeutic benefit. Chondroitinase Glycoproteins require both a substantial portion of the catalytic domain of the CHASEGP polypeptide and asparagine-linked glycosylation for optimal chondroitinase activity. The invention also includes carboxy-terminal deletion variants of CHASEGP that result in secreted variants of the protein to facilitate manufacture of a recombinant CHASEGP. Further described are suitable formulations of a substantially purified recombinant CHASEGP glycoprotein derived from a eukaryotic cell that generate the proper glycosylation required for its optimal activity. CHASEGP is useful for the degradation of glycosaminoglycans and chondroitin sulfate proteoglycans under clinical conditions where their removal is of therapeutic value.
    Type: Grant
    Filed: May 29, 2009
    Date of Patent: August 26, 2014
    Assignee: Halozyme, Inc.
    Inventors: Gregory I. Frost, Anirban Kundu, Louis H. Bookbinder
  • Publication number: 20140234231
    Abstract: Isolated ?-glucanases from Hypocrea tawa, Trichoderma reesei, and Trichoderma konilangbra are described, as well as oral care compositions containing the same. The oral care composition may be employed to prevent or reduce dental plaque.
    Type: Application
    Filed: February 27, 2014
    Publication date: August 21, 2014
    Applicant: DANISCO US INC.
    Inventors: Steven Kim, Suzanne Lantz, Michael Pepsin
  • Patent number: 8809018
    Abstract: Modified nucleotide molecules of xylanase and the application of the nucleotide molecules in constructing recombinant vectors, host cells or producing xylanase are disclosed, wherein the nucleotide molecules contain nucleotide sequences having greater than 80% identity with nucleotide sequence shown by SEQ ID NO: 1.
    Type: Grant
    Filed: February 10, 2010
    Date of Patent: August 19, 2014
    Assignee: YFY Biopulp Technology Limited
    Inventors: Yahui Huang, Chunhui Hsieh
  • Publication number: 20140227762
    Abstract: The present invention relates to a mannanase produced from Celluosimicrobium sp. strain HY-13 and more particularly to a highly active novel mannanase produced from Cellulosimicrobium sp. strain HY-13 as separated from an invertebrate. The mannanase produced from the said Cellulosimicrobium ap. strain HY-13 can be used in the decomposition of a mannan found in hemicellulose contained in grains and plants and in enhancing the utility value of a hydrolysate, which uses the decomposed mannan, for the transformation of biomass or as an animal feed material.
    Type: Application
    Filed: December 28, 2011
    Publication date: August 14, 2014
    Applicant: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Ho-Yong Park, Kwang-Hee Son, Do Young Kim, Dong-Ha Shin, Han-Young Cho
  • Publication number: 20140227761
    Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: February 12, 2013
    Publication date: August 14, 2014
    Applicants: NOVOZYMES INC., NOVOZYMES A/S
    Inventor: Nikolaj Spodsberg
  • Publication number: 20140227760
    Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: February 12, 2013
    Publication date: August 14, 2014
    Inventor: Nikolaj Spodsberg
  • Patent number: 8802412
    Abstract: The present invention relates to isolated polynucleotides having promoter activity the use of the isolated polynucleotides for the production of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity.
    Type: Grant
    Filed: June 23, 2011
    Date of Patent: August 12, 2014
    Assignee: Novozymes A/S
    Inventors: Hiroshi Teramoto, Hiroaki Udagawa, Rikako Taira
  • Patent number: 8802414
    Abstract: This document provides methods and materials related to reducing biofilms. For example, enzymes (e.g., glycosyl hydrolases), nucleic acid molecules encoding enzymes, host cells containing nucleic acid encoding enzymes, and methods for using enzymes to reduce biofilms and infections associated with biofilms are provided.
    Type: Grant
    Filed: February 6, 2013
    Date of Patent: August 12, 2014
    Assignee: Mayo Foundation for Medical Education and Research
    Inventors: Kristi L. Frank, Robin Patel
  • Patent number: 8802388
    Abstract: The present compositions and methods relate to an endo-?-mannanase cloned from Bacillus agaradhaerens, polynucleotides encoding the endo-?-mannanase, and methods of use thereof. Formulations containing the endo-?-mannanase are highly suitable for use as detergents.
    Type: Grant
    Filed: April 27, 2012
    Date of Patent: August 12, 2014
    Assignee: Danisco US Inc.
    Inventors: Brian E. Jones, Marc Kolkman, Zhen Qian, Brian Sogaard Laursen, Karsten M. Kragh, Sina Pricelius, Zheyong Yu, Lilia Maria Babe, Melodie Estabrook
  • Publication number: 20140221465
    Abstract: The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmRNA molecules.
    Type: Application
    Filed: February 3, 2014
    Publication date: August 7, 2014
    Applicant: Moderna Therapeutics, Inc.
    Inventors: Stephane Bancel, Tirtha Chakraborty, Antonin de Fougerolles, Susan Whoriskey
  • Publication number: 20140212948
    Abstract: A xylanase composition and a method for manufacturing the xylanase composition are provided, wherein the xylanase composition comprises a xylanase and a stabilizer, and the xylanase is from an anaerobic fungus, the stabilizer comprises a polyol, and the content of the polyol is at least 40 wt %, based on the total weight of the xylanase composition.
    Type: Application
    Filed: April 1, 2014
    Publication date: July 31, 2014
    Applicant: YFY Biopulp Technology Limited
    Inventors: Yahui HUANG, Chunhui HSIEH, Hsuanyu LAI
  • Publication number: 20140206086
    Abstract: The present invention relates to methods for obtaining positive transformants of a filamentous fungal host cell, comprising: transforming a tandem construct into a population of cells of the filamentous fungal host a tandem construct and isolating a transformant of the filamentous fungal host cell comprising the tandem construct. The present invention also relates to such tandem constructs, filamentous fungal host cells comprising such tandem constructs, and methods of producing multiple recombinant proteins.
    Type: Application
    Filed: August 23, 2012
    Publication date: July 24, 2014
    Applicant: Novozymes, Inc.
    Inventor: Donna Moyer
  • Publication number: 20140200263
    Abstract: The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmRNA molecules.
    Type: Application
    Filed: February 3, 2014
    Publication date: July 17, 2014
    Applicant: MODERNA THERAPEUTICS, INC.
    Inventors: Stephane Bancel, Tirtha Chakraborty, Antonin de Fougerolles, Susan Whoriskey
  • Patent number: 8778649
    Abstract: A thermophilic endo-beta-1,4-xylanase derived from Acidothermus cellulolyticus is disclosed. The xylanase exhibits xylanase activity at an optimal temperature of 90° C. and an optimal pH range of about 4.5-6.0. The isolated xylanase is useful in the hydrolysis of lignocellulosic material.
    Type: Grant
    Filed: October 16, 2012
    Date of Patent: July 15, 2014
    Assignee: The Regents of the University of California
    Inventors: Rebecca E. Parales, Alison M. Berry, Juanito V. Parales, Jr., Ravi D. Barabote
  • Patent number: 8778648
    Abstract: Compositions suitable for oral administration to an animal comprising at least one immune stress-reducing enzyme in an amount effective to decrease the level of positive acute phase protein in an animal, increase the level of negative acute phase protein in an animal, and/or improve animal growth performance is provided, as are methods using such compositions. The compositions include animal feed compositions, liquid compositions other than animal feed, and solid compositions other than animal feed.
    Type: Grant
    Filed: March 25, 2011
    Date of Patent: July 15, 2014
    Assignee: Eli Lilly and Company
    Inventors: David M. Anderson, Humg-Yu Hsiao, Lin Liu
  • Patent number: 8778640
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 15, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8778639
    Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 15, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8778641
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 15, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Publication number: 20140194494
    Abstract: The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmRNA molecules.
    Type: Application
    Filed: February 3, 2014
    Publication date: July 10, 2014
    Applicant: MODERNA THERAPEUTICS, INC.
    Inventors: Stephane Bancel, Tirtha Chakraborty, Antonin de Fougerolles, Susan Whoriskey
  • Patent number: 8772001
    Abstract: Processes for production of an alcohol product from granular starch comprising a pre-treatment at an elevated temperature below the initial gelatinization temperature of the granular starch followed by simultaneous saccharification and fermentation, and optionally recovery of ethanol, are described.
    Type: Grant
    Filed: June 13, 2008
    Date of Patent: July 8, 2014
    Assignee: Novozymes A/S
    Inventors: Hans Sejr Olsen, Sven Pedersen, Rikke Monica Festersen
  • Patent number: 8771993
    Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 8, 2014
    Assignees: Novozymes A/S, Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8771994
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 8, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8765438
    Abstract: Polypeptides with xylanase activity modified to increase bran solubilization and/or xylanase activity. The modification comprises modification of one or more amino acids in position 12 or 13 in combination with one or more further amino acid modifications in position 15, 34, 54, 77, 81, 82, 99, 104, 110, 113, 114, 118, 122, 141, 154, 159, 162, 164, 166, 175 or 179, wherein the positions are determined as the position corresponding the position of Bacillus subtilis xylanase (SEQ ID NO 1).
    Type: Grant
    Filed: December 23, 2009
    Date of Patent: July 1, 2014
    Assignee: DuPont Nutrition Biosciences ApS
    Inventors: Ole Sibbesen, Jens Frisbæk Sørensen
  • Publication number: 20140178350
    Abstract: Provided are substantially dephosphorylated forms of lysosomal storage disease (LSD) proteins, including dephosphorylated forms of iduronate-2-sulfatase (IDS, or I2D) and iduronidase (IDU), having increased ability to traverse or penetrate the blood brain barrier (BBB) relative to phosphorylated forms of the protein, and p97 conjugates thereof. Also provided are compositions comprising such dephosphorylated LSD proteins and p97 conjugates, and methods of use thereof, for instance, to treat any one or more lysosomal storage diseases, such as Hunter Syndrome (or MPS Type II).
    Type: Application
    Filed: July 31, 2013
    Publication date: June 26, 2014
    Applicant: biOasis Technologies, Inc.
    Inventors: Timothy Z. Vitalis, Reinhard Gabathuler
  • Publication number: 20140178933
    Abstract: An expression vector which is capable of overexpressing a protein of interest in a host cell, a host cell comprising the expression vector, and a method of producing a protein of interest are provided.
    Type: Application
    Filed: April 30, 2012
    Publication date: June 26, 2014
    Applicants: RESEARCH & BUSINESS FOUNDATION SUNGKYUNKWAN UNIVERSITY, Samsung Electronics Co., Ltd.
    Inventors: Hyun Min Koo, Byung Jo Yu, Ki Sung Lee, Jae Chan Park, Dae Hyuk Kweon, Sung Minm Park
  • Patent number: 8759041
    Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: June 24, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8759040
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: June 24, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8759023
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: January 26, 2012
    Date of Patent: June 24, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8753864
    Abstract: The present invention relates to recombinant N-glycosylated proteins, comprising one or more introduced N-glycosylated optimized amino acid sequence(s), nucleic acids encoding these proteins as well as corresponding vectors and host cells. In addition, the present invention is directed to the use of said proteins, nucleic acids, vectors and host cells for preparing medicaments. Furthermore, the present invention provides methods for producing said proteins.
    Type: Grant
    Filed: May 10, 2006
    Date of Patent: June 17, 2014
    Assignee: ETH Zurich
    Inventors: Markus Aebi, Michael Kowarik, Umesh Ahuja
  • Patent number: 8753860
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: June 17, 2014
    Assignee: Novozymes A/S
    Inventors: Marc D. Morant, Paul Harris
  • Patent number: 8748150
    Abstract: The present invention provides methods and compositions to reduce growth of microbial colonies, including infections, and includes therapeutic compositions, methods for treatment of infections, and methods for identifying additional such compositions.
    Type: Grant
    Filed: May 21, 2012
    Date of Patent: June 10, 2014
    Assignee: GangaGen, Inc.
    Inventors: Sriram Padmanabhan, Vivek Daniel Paul, R. Sanjeev Saravanan, Bharathi Sriram
  • Patent number: 8748149
    Abstract: The invention relates to the use of one or more pectinolytic enzyme(s) for the treatment of fruit or vegetable mash as well as a process for enzymatic treatment of fruit or vegetable mash comprising the step of adding one or more pectinolytic enzyme(s), wherein at least one pectinolytic enzyme is obtainable from Trichoderma reesei, as well as to a process for the preparation of a fruit or vegetable juice comprising the process for enzymatic treatment of fruit or vegetable mash. Moreover, the invention discloses recombinant DNA molecules encoding a polypeptide having endo-polygalacturonase activity, a polypeptide having exo-polygalacturonase activity, a polypeptide having exo-rhamnogalacturonase activity and a polypeptide having xylogalacturonase activity.
    Type: Grant
    Filed: May 22, 2009
    Date of Patent: June 10, 2014
    Assignee: AB Enzymes GmbH
    Inventors: Terhi Puranen, Bernhard Seiboth, Klaudija Milos, Wilfried Theiss, Jarno Kallio, Christian Kubicek, Jari Vehmaanperä
  • Publication number: 20140154707
    Abstract: Transformed Phaeodactylum tricornutum including a nucleic acid sequence operatively linked to a promoter, wherein the nucleic acid sequence encodes an N-acetylglucosaminyltransferase I and/or an ?-Mannosidase II and wherein at least one ?-N-acetylglucosaminidase of the transformed Phaeodactylum tricornutum has been inactivated. A method for producing a glycosylated polypeptide includes the steps of (i) culturing a transformed P. tricornutum as defined previously and (ii) purifying the polypeptide that is expressed and glycosylated in the transformed P. Tricornutum. The use of such a transformed P. tricornutum for producing a glycosylated polypeptide is also described.
    Type: Application
    Filed: July 27, 2011
    Publication date: June 5, 2014
    Applicants: UNIVERSITE DE ROUEN, L'INSTITUT FRANCAIS DE RECHERCHE POUR L'EXPLOITATION DE LA MER (IFREMER), ALGENICS
    Inventors: Muriel Bardor, Romain Louvet, Bruno Saint-Jean, Carole Burel, Berangere Baiet, Jean-Paul Cadoret, Patrice Lerouge, Remy Michel, Aude Carlier
  • Patent number: 8741174
    Abstract: A reduced weight DF-200 decontamination formulation that is stable under high temperature storage conditions. The formulation can be pre-packed as an all-dry (i.e., no water) or nearly-dry (i.e., minimal water) three-part kit, with make-up water (the fourth part) being added later in the field at the point of use.
    Type: Grant
    Filed: May 21, 2008
    Date of Patent: June 3, 2014
    Assignee: Sandia Corporation
    Inventor: Mark D. Tucker
  • Patent number: 8735118
    Abstract: A xylanase composition and a method for manufacturing the xylanase composition are provided, wherein the xylanase composition comprises a xylanase and a stabilizer, and the xylanase is from an anaerobic fungus, the stabilizer comprises a polyol, and the content of the polyol is at least 40 wt %, based on the total weight of the xylanase composition.
    Type: Grant
    Filed: November 24, 2009
    Date of Patent: May 27, 2014
    Assignee: YFY Biopulp Technology Limited
    Inventors: Yahui Huang, Chunhui Hsieh, Hsuanyu Lai
  • Patent number: 8735129
    Abstract: The present invention relates to a novel ginsenoside glycosidase protein derived from the genus Terrabacter, the protein having an activity of converting protopanaxadiol (PPD)-type saponins into highly active substances, which can be absorbed inside the body, by selective hydrolysis of a particular bond of ginsenoside. More specifically, the present invention relates to an amino acid sequence of the protein, a nucleic acid sequence encoding the protein, a recombinant vector comprising the nucleic acid sequence, and a transformant transformed with the vector, and a method for producing ginsenoside glycosidase derived from the genus Terrabacter by culturing the transformant, a method for converting PPD-type major saponins into the minor saponin forms using the protein, and a composition for converting PPD-type saponins into soluble saponins, comprising the protein as an active component.
    Type: Grant
    Filed: April 23, 2012
    Date of Patent: May 27, 2014
    Assignee: Korea Research Institute of Bioscience and Biotechnology
    Inventors: Dong Shan An, Song Gun Kim, Sung Taik Lee, Wan Taek Im, Hyung Gwan Lee, Sun Chang Kim
  • Patent number: 8735563
    Abstract: The present invention relates to isolated polynucleotides having promoter activity the juse of the isolated polynucleotides for the procuction of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity.
    Type: Grant
    Filed: June 23, 2011
    Date of Patent: May 27, 2014
    Assignee: Novozymes A/S
    Inventors: Hiroshi Teramoto, Hiroaki Udagawa
  • Patent number: 8735128
    Abstract: The present invention relates to isolated polypeptides having alpha-glucuronidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: July 29, 2009
    Date of Patent: May 27, 2014
    Assignee: Novozymes A/S
    Inventors: Michelle Maranta, Kimberly Brown