Abstract: A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock and enzymes for use in the process are provided. The process comprises partially hydrolyzing an aqueous slurry of a pretreated lignocellulosic feedstock with cellulase enzymes, one or more than one ?-glucosidase enzyme and a binding agent for binding the ?-glucosidase enzyme to fiber solids present in the aqueous slurry. The unhydrolyzed fiber solids are then separated from the hydrolyzed slurry. The separated fiber solids thus obtained are then re-suspended in an aqueous solution to produce a re-suspended slurry. The hydrolysis is then continued to produce the hydrolysis product comprising glucose.
Type:
Grant
Filed:
June 22, 2007
Date of Patent:
June 19, 2012
Assignee:
Iogen Energy Corporation
Inventors:
Jeffrey Tolan, Theresa White, John Tomashek
Abstract: The present invention provides methods and compositions to reduce growth of microbial colonies, including infections, and includes therapeutic compositions, methods for treatment of infections, and methods for identifying additional such compositions.
Type:
Grant
Filed:
May 4, 2007
Date of Patent:
June 19, 2012
Assignee:
GangaGen, Inc.
Inventors:
Sriram Padmanabhan, Vivek Daniel Paul, R. Sanjeev Saravanan, Bharathi Sriram
Abstract: Disclosed are: transcription regulatory factors capable of regulating the transcription or expression of genes for mannanases or cellulases, as mentioned below; and others. Specifically disclosed is a protein selected from the following proteins (a), (b) and (c): (a) a protein comprising the amino acid sequence depicted in SEQ ID NO:2; (b) a protein which comprises an amino acid sequence produced by deleting, substituting or adding one or several amino acid residues (e.g., 1 to 5 amino acid residues) in the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases; and (c) a protein which comprises an amino acid sequence having a 70% or higher sequence identity to the amino acid sequence depicted in SEQ ID NO:2 and which is capable of regulating the transcription of genes for mannanases or cellulases, or a partial fragment of the protein. Also specifically disclosed are a gene encoding the protein, and others.
Abstract: Microspheres are produced by contacting a solution of a macromolecule or small molecule in a solvent with an antisolvent and a counterion, and chilling the solution. The microspheres are useful for preparing pharmaceuticals, nutraceuticals, cosmetic products and the like of defined dimensions.
Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.
Type:
Application
Filed:
February 15, 2012
Publication date:
June 7, 2012
Applicant:
BP Corporation North America Inc.
Inventors:
Edward J. Bylina, Ronald Swanson, Eric Mathur, David E. Lam
Abstract: The invention provides compositions and methods for enhancing clearance of coagulation factors {e.g., VWF) and platelets from the blood stream of a patient in need thereof. The methods comprise administering to the patient a therapeutically effective amount of an agent that increases clearance of coagulation factors or platelets. Such an agent can be, for example, a neuraminidase.
Type:
Grant
Filed:
October 10, 2008
Date of Patent:
May 29, 2012
Assignee:
The Regents of the University of California
Abstract: The present invention relates to a host cell comprising a cofilin-specific small interfering RNA (siRNA) sequence. The host cell may further comprise a nucleic acid encoding a recombinant protein. The present invention also relates to a method for producing a recombinant protein by the host cell comprising a cofilin-specific small interfering RNA (siRNA) sequence.
Abstract: Refractile particles containing a heterologous polypeptide as an insoluble aggregate are recovered from bacterial periplasm. The process involves culturing bacterial cells so as to express nucleic acid encoding phage lysozyme and nucleic acid encoding the heterologous polypeptide under separate promoters, disrupting the cells mechanically to release the phage lysozyme so as to release refractile particles from the bacterial cellular matrix, and recovering the released refractile particles from the periplasm. Chloroform is not used in any step and the recovery step minimizes co-recovery of cellular debris with the released refractile particles.
Abstract: A new strain Bacillus sp. P203 (Bacillus plakortiensis) is disclosed. Isolated mature functional polypeptide which is obtainable from the bacterium strain Bacillus sp. P203 deposited under accession number DSM 17419 are disclosed.
Type:
Application
Filed:
January 26, 2012
Publication date:
May 17, 2012
Applicant:
NOVOZYMES A/S
Inventors:
Preben Nielsen, Reinhard Wilting, Martin Borchert
Abstract: We describe a PS4 variant polypeptide derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 307 to lysine (K) or arginine (R), with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Preferably, the PS4 variant polypeptide further comprises an amino acid substitution at position 70, preferably G70D. The amino acid at positions 272 and 303 of the sequence of the are preferably histidine (H) and glycine (G).
Type:
Grant
Filed:
December 19, 2008
Date of Patent:
May 15, 2012
Assignee:
Danisco A/S
Inventors:
Patrick Maria Franciscus Derkx, Anja Kellet-Smith Hemmingen, Rie Mejldal, Bo Spange Sørensen, Karsten Matthias Kragh
Abstract: The present invention relates to a process for the production of one or more fermentation product from a sugar composition, comprising the following steps: a) fermentation of the sugar composition in the presence of a yeast belonging to the genera Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Kloeckera, Schwanniomyces or Yarrowia, and b) recovery of the fermentation product, wherein the yeast comprises the genes araA, araB and araD and the sugar composition comprises glucose, galactose and arabinose.
Type:
Application
Filed:
July 6, 2010
Publication date:
May 10, 2012
Applicant:
DSM IP ASSETS B.V.
Inventors:
Paul Klaassen, Gijsberdina Pieternella Van Suylekom, Bianca Elisabeth Maria Gielesen, Nicolette Jasmijn Broers, Beate Wiedemann, Wilhelmus Theodorus Antonius Maria De Laat
Abstract: A method for producing lacto-N-biose I and galacto-N-biose inexpensively and conveniently is provided. The method for producing lacto-N-biose I or galacto-N-biose, characterized in that the method comprises causing: (i) a combination of a carbohydrate raw material with an enzyme that catalyzes phosphorolysis of the carbohydrate raw material to give ?-glucose-1-phosphate; and (ii) a combination of an enzyme that converts ?-glucose-1-phosphate to UDP-glucose and an enzyme that converts UDP-galactose to galactose-1-phosphate with their cofactors, and/or a combination of an enzyme (UDP-Gly synthase) that converts ?-glucose-1-phosphate and UDP-galactose to UDP-glucose and ?-galactose-1-phosphate, respectively, with its cofactor(s) to act in the presence of N-acetylglucosamine or N-acetylgalactosamine, phosphoric acid, lacto-N-biose phosphorylase (EC 2.4.1.211), and UDP-glucose-4-epimerase (EC 5.1.3.2).
Type:
Grant
Filed:
December 12, 2007
Date of Patent:
May 8, 2012
Assignee:
Incorporated Administrative Agency National Agriculture and Food Research Organization
Abstract: Recombinant carrier molecules having amino acid sequences from thermostable enzymes and methods of use for expression, recovery and delivery of foreign sequences (peptides and polypeptides) produced in different systems (bacteria, yeast, DNA, cell cultures such as mammalian, plant, insect cell cultures, protoplast and whole plants in vitro or in vivo are provided. The recombinant carrier molecule using sequences from lichenase B(Lic B) were also made and used as part of carrier protein to express, recover and deliver a variety of target polypeptides of interest.
Type:
Grant
Filed:
November 24, 2009
Date of Patent:
May 8, 2012
Assignee:
Fraunhofer USA, Inc.
Inventors:
Vidadi Yusibov, Vadim Mett, Konstantin Musiychuk
Abstract: The present invention relates to fungal serine protease variants, which comprise an amino acid substitution of valine at position 208 of the parent Fusarium equiseti Fe_RF6318 serine protease, wherein the position of the substitution corresponds to the amino acid sequence of the mature Fe_RF6318 enzyme defined in SEQ ID NO:2. The variants have improved thermal stability and/or detergent stability compared to the parent Fe_RF6318 enzyme. Preferably the substitution is V208I and more preferably the variants comprise additional amino acid changes which further increase the stability. Also disclosed are nucleic acid sequences encoding said protease variants as well as recombinant vectors and host cells for the production of the variants.
Type:
Application
Filed:
October 28, 2011
Publication date:
May 3, 2012
Applicant:
AB ENZYMES OY
Inventors:
Kari Juntunen, Leena Valtakari, Nina Hakulinen, Marja Paloheimo
Abstract: The invention comprises a process for producing a chitosan derivative, and a product prepared thereby, by activating chitosan or a chitosan precursor chitin in alcohol that contains water from 0% to maximum of about 30% and preparing derivatives by reacting with a reagent to get derivatives that have various uses including an antimicrobial, or a microbiostatic, or an antitranspirant, or an antifungal, or a fruit preservative properties or as carrier of active molecules or functional groups etc. or a combination thereof. Illustrated derivatives include acid, succinic, enzymatically deacetylated, enzymatically hydrolysed by chitinolysis, sugar, formaldehyde, phosphoric acid, hydrochloric acid and copper sulfate derivatives that are obtained by reaction individually or in a combination of consecutive reactions.
Abstract: The present invention discloses a method for the enzyme-mediated, site-specific, in-vivo precipitation of a water soluble molecule in an animal. The enzyme is either unique to tumor cells, or is produced within a specific site (e.g., tumor) at concentrations that are higher than that in normal tissues. Alternatively, the enzyme is conjugated to a targeting moiety such as an antibody or a receptor-binding molecule.
Type:
Grant
Filed:
April 3, 2009
Date of Patent:
May 1, 2012
Assignee:
President and Fellows of Harvard College
Abstract: The present invention provides enriched polynucleotides, and enriched polypeptides having pectinase activity. The present invention also includes methods of using the polynucleotides and polypeptides described herein. For instance, the methods include producing a metabolic product, such as ethanol.
Type:
Application
Filed:
September 18, 2009
Publication date:
April 19, 2012
Applicant:
University of Georgia Research Foundation, Inc.
Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: Variants of Bacillus sp. TS-23 strain alpha-amylases exhibit improved enzymatic performance, including increased themostability, reduced calcium dependence, increased washing/cleaning performance, and baking ability. Compositions comprising these variants are useful in methods of starch processing, starch liquefaction, fermatation, starch saccharification, cleaning, laundrying, textile desizing, baking, and biofilm removal.
Type:
Grant
Filed:
November 3, 2008
Date of Patent:
April 10, 2012
Assignee:
Danisco US Inc.
Inventors:
Claudine Y. Chang, Clement Choy, Melodie Estabrook, Mansi Goyal, Thomas P. Graycar, Victoria E. Huang, Brian E. Jones, Marc Kolkman, Karsten M. Kragh, Chris Leeflang, Scott D. Power, Sandra W. Ramer, Andrew Shaw, Casper Vroemen, Walter Weyler
Abstract: The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.
Type:
Application
Filed:
November 2, 2011
Publication date:
April 5, 2012
Applicant:
Novozymes A/S
Inventors:
Kirk Schnorr, Per Lina Jorgensen, Martin Schulein, Hanne Dela
Abstract: The present invention relates to methods for degrading or converting a cellulosic material and for producing a substance from a cellulosic material.
Abstract: The invention relates to a xylanase originating from a Bacillus strain. This xylanase is active over a wide range of acid and basic pH. The invention also relates to new strains of microorganisms producing this xylanase and to methods for preparing this xylanase. The invention also relates to a DNA molecule and to an expression vector or an integration vector containing this DNA molecule. The invention also relates to uses of the latter and to compositions containing it.
Type:
Grant
Filed:
August 20, 2007
Date of Patent:
April 3, 2012
Assignee:
Danisco US Inc.
Inventors:
Eric De Buyl, Andrée Lahaye, Pierre Ledoux, René Detroz
Abstract: The present invention comprises a method of concentrating a composition comprising a polypeptide of interest and the use of such a concentrated composition for the treatment of diseases in mammals, in particular by subcutaneous injection.
Abstract: This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered exospecifity.
Type:
Grant
Filed:
July 7, 2004
Date of Patent:
March 27, 2012
Assignee:
Danisco A/S
Inventors:
Karsten Matthias Kragh, Kirsten Bojsen, Casper Tune Berg, Patrick M. F. Derkx, Anja H. Kellett-Smith, Charlotte Refdahl Thoudahl, Carol Fioresi, Wei Liu, Andrew Shaw, Gijsbert Gerritse
Abstract: The invention relates to Streptomyces koganeiensis ATCC 31394 hyaluronidase having molecular weight of 21.6 kDalton, which has hyaluronidase activity and stability markedly higher than those of the hyaluronidase obtained from such microorganism to date. The invention further relates to a process for the isolation and purification of said hyaluronidase and its use for the preparation of pharmaceutical compositions or as an analytical reagent.
Type:
Application
Filed:
May 12, 2010
Publication date:
March 22, 2012
Applicant:
KETER PLASTIC LTD.
Inventors:
Luciano Messina, Susana Vaccaro, Salvatore Caruso, Giovanni Gennari
Abstract: A chondroitin polymerase having such properties that it transfers GlcUA and GalNAc alternately to a non-reduced terminal of a sugar chain from a GlcUA donor and a GalNAc donor, respectively, and the like; and a process for producing the chondroitin polymerase.
Type:
Grant
Filed:
August 10, 2009
Date of Patent:
March 20, 2012
Assignee:
Seikagaku Corporation
Inventors:
Toshio Ninomiya, Nobuo Sugiura, Koji Kimata
Abstract: The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene.
Type:
Grant
Filed:
April 11, 2011
Date of Patent:
March 20, 2012
Assignee:
Novozymes, Inc.
Inventors:
Alan Sloma, Leslie Naggiar, legal representative, Regine Behr, William Widner, Maria Tang, David Sternberg, Linda Sternberg, legal representative, Stephen Brown
Abstract: Variant polypeptides derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the variant polypeptides comprise an amino acid mutation at one or more positions selected from the group consisting of: 121, 161, 223, 146, 157, 158, 198, 229, 303, 306, 309, 316, 353, 26, 70, 145, 188, 272, 339, with reference to the position numbering of a Pseudomonas saccharophila exoamylase sequence shown as SEQ ID NO: 1.
Type:
Grant
Filed:
July 7, 2005
Date of Patent:
March 20, 2012
Assignee:
Danisco A/S
Inventors:
Casper Tune Berg, Patrick M. F. Derkx, Carol Fioresi, Gijsbert Gerritse, Anja Hemmingen Kellet-Smith, Karsten Matthias Kragh, Wei Liu, Andrew Shaw, Bo Spange Sørensen, Charlotte Refdahl Thoudahl
Abstract: Described herein are composite materials and methods of using them for the separation or purification of enantiomers. In certain embodiments, the composite material comprises a support member, comprising a plurality of pores extending through the support member; and a macroporous cross-linked gel, comprising a plurality of macropores, and a plurality of pendant chiral moieties. In certain embodiments, the composite materials may be used in the separation or purification of a chiral small molecule.
Type:
Application
Filed:
September 13, 2011
Publication date:
March 15, 2012
Inventors:
Elena N. Komkova, Amro Ragheb, Charles H. Honeyman
Abstract: The present invention relates to a method of expressing a foreign protein in the plastid of a host cell and secreting said protein into the cytoplasm of the host cell comprising the steps of making a construct of vector linked to a coding sequence of the fusion protein comprising of signal peptide sequence followed by in-frame fusion to a foreign gene; and stably integrating said construct into the plastid genome. The present invention also relates to a method of targeting the expressed and secreted proteins from the plastids to the nucleus of the host cell. The present invention further relates to the method where the host cell is of any higher plant or any organism including single cell algae.
Type:
Application
Filed:
November 27, 2009
Publication date:
March 8, 2012
Inventors:
Vanga Siva Reddy, Sadhu Leelavathi, Amit Bhardwaj
Abstract: The invention relates to an improved enzyme complex having a plurality of enzyme activities of an expression product obtained by fermentation of the genus Trichoderma in combination with one or more enzymes of a different fungus strain.
Type:
Application
Filed:
May 7, 2010
Publication date:
March 8, 2012
Applicant:
Danisco A/S
Inventors:
Neville Marshall Fish, Lone Brønd Miller
Abstract: Provided herein are glycosylated polypeptide compositions with substantially reduced Neu5Gc content. The glycosylated polypeptides compositions with substantially reduced Neu5Gc content can be obtained from cell sources cultured with Neu5Gc competitor or from non-human animal sources fed a diet supplemented with Neu5Gc competitor. Also provided herein are methods of treating a human subject with said compositions.
Abstract: A thermostable glycosidase enzymes derived from various Thermococcus, Staphylothermus and Pyrococcus organisms is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry, pharmaceutical industry and in the textile industry, detergent industry and in the baking industry.
Type:
Grant
Filed:
August 20, 2010
Date of Patent:
February 21, 2012
Assignee:
BP Corporation North America Inc.
Inventors:
Edward J. Bylina, Ronald Swanson, Eric Mathur, David E. Lam
Abstract: Hemicellulase (xylanase) enzymes possessing endo-xylanase, laminarase, mannanase, arabinase and arabinofuranosidase activity are useful to degrade hemicellulose and other substrates to their constituent sugars.
Type:
Application
Filed:
January 19, 2010
Publication date:
February 16, 2012
Applicant:
THE BOARD OF REGENTS FOR OKLAHOMA STATE UNIVERSITY
Abstract: This disclosure provides novel reversibly terminated ribonucleotides which can be used as a reagent for DNA sequencing reactions. Methods of sequencing nucleic acids using the disclosed nucleotides are also provided.
Type:
Application
Filed:
August 10, 2011
Publication date:
February 16, 2012
Applicant:
ALERE SAN DIEGO, INC.
Inventors:
Olaf Piepenburg, Derek L. Stemple, Niall A. Armes
Abstract: This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, deinking and biobleaching of paper and pulp, and treatment of waste streams.
Type:
Application
Filed:
April 5, 2011
Publication date:
February 9, 2012
Applicant:
DYADIC INTERNATIONAL , INC.
Inventors:
Alexander Vasilievich Gusakov, Peter J. Punt, Jan Cornelis Verdoes, Arkady Panteleimonovich Sinitsyn, Elena Vlasenko, Sandra Wilhelmina Agnes Hinz, Mark Gosink, Zhijie Jiang, Jacoba Van der Meij
Abstract: Method for providing a hypoallergenic glycoprotein includes growing at least one of a mutated plant, a part of the mutated plant, plant cells produced from the mutated plant, a transgenic plant, a part of the transgenic plant and plant cells produced from the transgenic plant wherein an activity of an enzyme Golgi ?-mannosidase II has been eliminated or decreased so as to obtain a grown material. The hypoallergenic glycoprotein is isolated from the grown material.
Abstract: The present invention relates to the intersection of the fields of immunology and protein engineering, and particularly to antigens and compositions useful in inducing or enhancing an immune response agains influenza antigens. Provided are recombinant protein antigens, compositions, and methods for the production of such antigens in plants. In some embodiments, influenza antigens include hemagglutinin polypeptides, neuraminidase polypeptides, and/or combinations thereof.
Type:
Application
Filed:
September 25, 2009
Publication date:
February 9, 2012
Applicant:
FRAUNHOFER USA, INC.
Inventors:
Vidadi Yusibov, Vadim Mett, Konstantin Musiychuk
Abstract: This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, deinking and biobleaching of paper and pulp, and treatment of waste streams.
Type:
Application
Filed:
April 5, 2011
Publication date:
February 2, 2012
Applicant:
DYADIC INTERNATIONAL, INC.
Inventors:
Alexander Vasilievich Gusakov, Peter J. Punt, Jan Cornelis Verdoes, Arkady Panteleimonovich Sinitsyn, Elena Vlasenko, Sandra Wilhelmina Agnes Hinz, Mark Gosink, Zhijie Jiang, Jacobs Van der Meij
Abstract: The invention relates to a process for production of an enzyme product having a plurality of enzyme activities obtained by fermentation of an Aspergillus strain.
Abstract: Polypeptides with xylanase activity modified to increase bran solubilization and/or xylanase activity. The modification comprises modification of one or more amino acids in position 12 or 13 in combination with one or more further amino acid modifications in position 15, 34, 54, 77, 81, 82, 99, 104, 110, 113, 114, 118, 122, 141, 154, 159, 162, 164, 166, 175 or 179, wherein the positions are determined as the position corresponding the position of Bacillus subtilis xylanase (SEQ ID NO 1).
Abstract: The present invention relates to isolated polypeptides having alpha-L-arabinofuranosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Type:
Application
Filed:
October 10, 2011
Publication date:
February 2, 2012
Applicant:
NOVOZYMES A/S
Inventors:
Michelle Maranta, Kimberly Brown, James Langston
Abstract: The invention relates to the discovery of novel soluble neutral active Hyaluronidase Glycoproteins (sHASEGPs), methods of manufacture, and their use to facilitate administration of other molecules or to alleviate glycosaminoglycan associated pathologies. Minimally active polypeptide domains of the soluble, neutral active sHASEGP domains are described that include asparagine-linked sugar moieties required for a functional neutral active hyaluronidase domain. Included are modified amino-terminal leader peptides that enhance secretion of sHASEGP. The invention further comprises sialated and pegylated form of a recombinant sHASEGP to enhance stability and serum pharmacokinetics over naturally occurring slaughterhouse enzymes. Further described are suitable formulations of a substantially purified recombinant sHASEGP glycoprotein derived from a eukaryotic cell that generate the proper glycosylation required for its optimal activity.
Type:
Grant
Filed:
June 15, 2010
Date of Patent:
January 31, 2012
Assignee:
Halozyme, Inc.
Inventors:
Louis H. Bookbinder, Anirban Kundu, Gregory I. Frost
Abstract: The present invention relates to a process for enzymatic hydrolysis of granular starch into a soluble starch hydrolysate at a temperature below the initial gelatinization temperature of said granular starch.
Type:
Grant
Filed:
June 25, 2004
Date of Patent:
January 31, 2012
Assignee:
Novozymes A/S
Inventors:
Anders Vikso Nielsen, Carsten Andersen, Sven Pedersen, Carsten Hjort
Abstract: Polypeptides with xylanase activity modified to increase bran solubilisation and/or xylanase activity. The modification comprises modification of one or more amino acids in position 113, 122 or 175 in combination with one or more further amino acid modifications in position 11, 12, 13, 34, 54, 77, 81, 82, 104, 110, 113, 118, 122, 141, 154, 159, 162, 164, 166, 175 or 179, wherein the positions are determined as the position corresponding to the position of Bacillus subtilis xylanase (SEQ ID NO 1).
Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Type:
Application
Filed:
June 30, 2011
Publication date:
January 26, 2012
Applicants:
Novozymes, Inc., Novozymes A/S
Inventors:
Marc D. Morant, Shamkant Patkar, Hanshu Ding