Pichia Patents (Class 435/254.23)
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Patent number: 8741621Abstract: Recombinant P. pastoris producing natural sweet proteins and methods for engineering these recombinant yeast are described. Methods for enhancing foreign protein production in yeast fermentation and improved methods for purification of foreign proteins produced in yeast fermentation are presented.Type: GrantFiled: October 1, 2009Date of Patent: June 3, 2014Assignee: Tate & Lyle Technology LimitedInventors: Alfred Carlson, Richard W. Armentrout, Timothy Peter Ellis
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Publication number: 20140147436Abstract: The present invention concerns polypeptides comprising a variant Fc region. More particularly, the present invention concerns Fc region-containing polypeptides that have altered effector function as a consequence of one or more amino acid modifications in the Fc region thereof.Type: ApplicationFiled: July 19, 2013Publication date: May 29, 2014Applicant: GENENTECH, INC.Inventor: Leonard G. PRESTA
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Publication number: 20140150137Abstract: The present invention relates to isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.Type: ApplicationFiled: April 26, 2012Publication date: May 29, 2014Applicant: Novozymes, Inc.Inventors: Nikolaj Spodsberg, Brett McBrayer, Pierre Cassland
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Publication number: 20140147893Abstract: The present invention relates to variants of alpha amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: ApplicationFiled: July 6, 2012Publication date: May 29, 2014Applicant: NOVOZYMES A/SInventors: Tomoko Matsui, Aki Tomiki, Guillermo Coward-Kelly
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Publication number: 20140147907Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials, to proceed ethanol and/or butanol, e.g., by fermentation.Type: ApplicationFiled: January 31, 2014Publication date: May 29, 2014Applicant: XYLECO, INC.Inventors: Marshall Medoff, Thomas Craig Masterman
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Publication number: 20140147458Abstract: The present invention relates to novel Varicella-zoster virus strain and to a chicken pox and herpes zoster virus vaccine using the same. More particularly, the present invention relates to genome DNA of VZV MAV/06 strain isolated from a Korean patient, to an open reading frame (ORF) thereof, to a protein coded by the genome DNA of VZV MAV/06 strain and the ORF thereof, and to a vaccine composition which contains the protein as an active ingredient.Type: ApplicationFiled: February 24, 2012Publication date: May 29, 2014Applicant: MOGAM BIOTECHNOLOGY RESEARCH INSTITUTEInventors: Geun Hee Kim, Shi Nae Kwon, Chan Hee Lee, Yeop Yoon
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Publication number: 20140147894Abstract: The present disclosure relates to variant CBH I polypeptides that have reduced product inhibition, and compositions, e.g., cellulase compositions, comprising variant CBH I polypeptides. The variant CBH I polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH I polypeptides and host cells that recombinantly express the variant CBH I polypeptides.Type: ApplicationFiled: October 6, 2011Publication date: May 29, 2014Applicant: BP Corporation North America Inc.Inventors: Justin T. Stege, Alexander Varvak, John Poland, Chris S. Lyon, Shaun Healey, Peter Luginhuhl
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Publication number: 20140141012Abstract: The invention relates to binding compounds that specifically bind to human TSLP, as well as uses thereof, e.g., in the treatment of inflammatory disorders and allergic inflammatory response.Type: ApplicationFiled: January 28, 2014Publication date: May 22, 2014Applicant: MERCK SHARP & DOHME CORP.Inventor: Leonard G. Presta
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Publication number: 20140140933Abstract: The present disclosure provides antibodies that bind complement C1s protein; and nucleic acid molecules that encode such antibodies. The present disclosure also provides compositions comprising such antibodies, and methods to produce and use such antibodies, nucleic acid molecules, and compositions.Type: ApplicationFiled: November 1, 2013Publication date: May 22, 2014Inventors: Peter Van Vlasselaer, Graham Parry, Nancy E. Stagliano, Sandip Panicker
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Publication number: 20140140999Abstract: The invention provides means and methods for producing one or more Ig-like molecules in a single host cell. Novel CH3 mutations enabling the production of monospecific and/or bispecific Ig-like molecules of interest are also provided.Type: ApplicationFiled: November 15, 2013Publication date: May 22, 2014Applicant: MERUS B.V.Inventors: Cornelis A. DE KRUIF, Linda Johanna Aleida HENDRIKS, Ton LOGTENBERG
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Publication number: 20140142286Abstract: A method for producing recombinant polypeptides, including antibodies, having targeted levels of carboxyl terminal (C-terminal) lysine or arginine residues is presented. The method involves culturing cells expressing said polypeptides in cell culture medium having sufficient levels of lysine, arginine, and/or agents that change intracellular pH. Culturing the cells under such conditions does not affect cell growth, cell viability, or titer.Type: ApplicationFiled: July 6, 2012Publication date: May 22, 2014Applicant: MOMENTA PHARMACEUTICALS, INC.Inventor: Holly Prentice
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Publication number: 20140141507Abstract: Methods are provided for the expression of immunoglobulin variable domains that are secreted into the culture medium. The methods provide for the production of homogeneous immunoglobulin variable domains in which the proportion of product-related variants that comprise, at the N-terminus, at least one redundant amino acid residue derived from the secretion signal is strongly reduced or absent.Type: ApplicationFiled: May 9, 2012Publication date: May 22, 2014Applicant: Ablynx N.V.Inventors: Ann Brigé, Peter Schotte, Bart Walcarius
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Publication number: 20140141478Abstract: The invention provides a process for reacting a carboxylic acid ester of the formula (I) R1-A-COOR2 ??(I), wherein R1 is hydrogen, —CH2OH, —CHO, —COOR3, —CH2SH, —CH2OR3 or —CH2NH2, R2 is an alkyl group,R3 is hydrogen or an alkyl group, and A is a substituted, unsubstituted, linear, branched and/or cyclic alkylene, alkenylene, arylene or aralkylene radical having at least 4 carbons,in the presence of a cell. The process comprises a) contacting the cell with said carboxylic acid ester in an aqueous solution,wherein the cell is a recombinant cell which has reduced activity of a polypeptide comprising SEQ ID NO: 2 or a variant thereof over the wild-type cell.Type: ApplicationFiled: November 12, 2013Publication date: May 22, 2014Applicant: Evonik Industries AGInventors: Steffen SCHAFFER, Heiko Andrea, Mirja Wessel, Hans-Georg Hennemann, Harald Haeger
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Publication number: 20140141482Abstract: This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.Type: ApplicationFiled: June 12, 2013Publication date: May 22, 2014Applicant: INVISTA North America S.á r.l.Inventors: Paul S. Pearlman, Changlin Chen, Adriana Leonora Botes, Alex Van Eck Conradie
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Publication number: 20140142046Abstract: The invention provides novel Wnt polypeptides that have enhanced solubility and improved biologic drug-like properties, and polynucleotides encoding the Wnt polypeptides of the invention. The Wnt polypeptides of the invention can be used therapeutically, such as, for example, in methods of preventing or treating muscle loss and/or promoting muscle hypertrophy and growth.Type: ApplicationFiled: January 11, 2012Publication date: May 22, 2014Applicant: FATE THERAPEUTICS, INC.Inventors: Tom Tong Lee, Monica Hayhurst Bennett, Michael J. Fitch, Peter Flynn
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Publication number: 20140134736Abstract: The present disclosure relates to methods of controlling the sulfide (S2˜) content in systems, such as oil and gas reservoirs and pipelines, by the use of chlorine oxyanions and microorganisms with (per)chlorate-reducing activity.Type: ApplicationFiled: May 31, 2012Publication date: May 15, 2014Applicant: The Regents of the University of CaliforniaInventor: John D. Coates
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Publication number: 20140134687Abstract: The present disclosure generally relates to microorganisms (e.g., non-naturally occurring microorganisms) that comprise one or more polynucleotides coding for enzymes in a pathway that catalyze a conversion of a carbon source (e.g., a fermentable carbon source) to butadiene and succinate and the use of such microorganisms for the production of butadiene and succinate.Type: ApplicationFiled: October 2, 2013Publication date: May 15, 2014Applicant: Braskem S/A AP 09Inventors: Mateus Schreiner Garcez Lopes, Avram Michael Slovic
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Publication number: 20140134681Abstract: The invention relates to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’) using a biocatalyst. The invention further relates to a method for preparing e-caprolactam (hereafter referred to as ‘caprolactam’) by cyclising such 6-ACA. The invention further relates to a host cell, a micro-organism, or a polynucleotide which may be used in the preparation of 6-ACA or caprolactam.Type: ApplicationFiled: December 13, 2013Publication date: May 15, 2014Inventors: Petronella Catharina RAEMAKERS-FRANKEN, Martin SCHURMANN, Axel Christoph TREFZER, Stefaan Marie Andre DE WILDEMAN
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Publication number: 20140134155Abstract: The invention relates to recombinant antibodies which bind to prostate-specific stem cell antigen (PSCA). The antibody of the invention comprises complementarity-determining regions (CDR) having the following amino acid sequences: CDR of the variable region of the light chain: CDR1 SEQ ID No. 1, CDR2 SEQ ID No. 2, CDR3 SEQ ID No. 3, and CDR of the variable region of the heavy chain: CDR1 SEQ ID No. 4, CDR2 SEQ ID No. 5, CDR3 SEQ ID No. 6. The invention also embraces the use of the antibodies of the invention as medicinal products, especially for the treatment of tumour diseases, or as a diagnostic agent. The antibodies are suitable for use in the areas of medicine, pharmacy and biomedical research.Type: ApplicationFiled: June 29, 2012Publication date: May 15, 2014Applicant: Technische Universität DresdenInventor: Michael Bachmann
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Publication number: 20140134183Abstract: The present invention relates to anti-IL-1 beta binding members and in particular to monovalent high potency IL-1 beta-binding antibody fragments being highly stable and soluble. Such binding members may be used in the treatment of inflammatory and other diseases as well as in diagnostics. Also provided are related nucleic acids, vectors, cells, and compositions.Type: ApplicationFiled: November 5, 2013Publication date: May 15, 2014Applicant: Delenex Therapeutics AGInventors: Stefanie Grabulovski, Titus Kretzschmar, Simone Schmitt, Abdijapar Shamshiev
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Publication number: 20140127770Abstract: The invention features methods producing isoprene and a co-product, such as ethanol, 1,3-propanediol, or hydrogen from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides compositions comprising isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen. Additionally, the invention provides methods of co-producing isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen by culturing cells under conditions suitable for co-production of isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen.Type: ApplicationFiled: January 6, 2014Publication date: May 8, 2014Applicants: The Goodyear Tire & Rubber Company, Danisco US Inc.Inventors: Gopal K. CHOTANI, Joseph C. MCAULIFFE, Caroline M. PERES, Karl J. SANFORD, Derek H. WELLS
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Publication number: 20140127759Abstract: A ?-glucosidase exhibits high activity in the presence of biomass and has high thermal stability compared to conventional enzymes. The ?-glucosidase includes substitutions and/or deletions of amino acids, at least three amino acids selected from the group consisting of Glu39, Asp169, Arg170, Arg220, Tyr227, and Glu330, of the parent ?-glucosidase with other amino acids and exhibits a decomposition activity.Type: ApplicationFiled: January 14, 2014Publication date: May 8, 2014Applicant: Toray Industries, Inc.Inventors: Hiroyuki Kurihara, Katsushige Yamada, Kazuhiko Ishikawa, Yumiko Mishima, Yasunobu Wada, Yuji Kado
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Patent number: 8715963Abstract: Described is a method for increasing the N-glycosylation site occupancy of a therapeutic glycoprotein produced in recombinant host cells modified as described herein and genetically engineered to express the glycoprotein compared to the N-glycosylation site occupancy of the therapeutic glycoprotein produced in a recombinant host cell not modified as described herein. In particular, the method provides recombinant host cells that overexpress a heterologous single-subunit oligosaccharyltransferase, which in particular embodiments is capable of functionally suppressing the lethal phenotype of a mutation of at least one essential protein of the yeast oligosaccharyltransferase (OTase) complex, for example, the Leishmania major STT3D protein, in the presence of expression of the host cell genes encoding the endogenous OTase complex.Type: GrantFiled: February 23, 2011Date of Patent: May 6, 2014Assignee: Merck Sharp & Dohme Corp.Inventors: Natarajan Sethuraman, Byung-Kwon Choi, Bianka Prinz, Michael Meehl, Terrance Stadheim
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Publication number: 20140120595Abstract: Three biosynthetic pathways are disclosed for microorganism bio-production of 1,4-Butanediol from various carbon sources. Exemplary methods are provided. The recombinant microorganisms comprising any of these 1,4-Butanediol biosynthesis pathways may also comprise genetic modifications directed to improved tolerance for 1,4-Butanediol.Type: ApplicationFiled: September 27, 2013Publication date: May 1, 2014Applicant: OPX BIOTECHNOLOGIES, INC.Inventor: MICHAEL D. LYNCH
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Publication number: 20140120598Abstract: Provided is a novel method of producing ethanol by using a cellulose-based biomass as a raw material. In particular, provided is a novel method of producing ethanol by which ethanol can be effectively produced in the presence of a substance having an inhibitory action on fermentation of ethanol. Ethanol can be effectively produced by using a microorganism engineered to suppress the expression of at least one kind of phosphatase among the phosphatases intrinsically possessed by the microorganism, even under a condition where a substance that has heretofore been believed to have a fermentation inhibitory action, specifically, a weakly acidic substance and/or a furan compound are/is incorporated.Type: ApplicationFiled: June 25, 2012Publication date: May 1, 2014Applicant: National University Corporation KOBE UniversityInventors: Akihiko Kondo, Tomohisa Hasunuma
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Publication number: 20140120566Abstract: The present invention relates to a novel bilirubin oxidase from Magnaporthe oryzae, to the method of preparation thereof as well as use thereof notably for assay of bilirubin and for the application of enzymatic biofuel cells.Type: ApplicationFiled: May 22, 2012Publication date: May 1, 2014Applicant: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUEInventors: Nicolas Mano, Fabien Durand
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Publication number: 20140121153Abstract: The present invention relates to an isolated polypeptide comprising (a) the amino acid sequence set forth in SEQ ID NO: 21; or (b) an amino acid sequence that is at least 95% identical to SEQ ID NO: 21, and to therapeutic treatments based thereon.Type: ApplicationFiled: January 2, 2014Publication date: May 1, 2014Inventors: Tamara SANDLER, Orly DEVARY
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Patent number: 8709756Abstract: Methods are provided for the synthesis and secretion of recombinant hetero-multimeric proteins in mating competent yeast. A first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.Type: GrantFiled: August 30, 2012Date of Patent: April 29, 2014Assignees: Keck Graduate Institute, Alder Biopharmaceuticals, Inc.Inventors: James M. Cregg, John Latham, Mark Litton, Randall Schatzman, Ilya I. Tolstorukov
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Publication number: 20140112910Abstract: Sulfated glycoproteins, and methods of making and using such sulfated glycoproteins, are described.Type: ApplicationFiled: May 18, 2012Publication date: April 24, 2014Applicant: MOMENTA PHARMACEUTICALS, INC.Inventors: Dorota A. Bulik, Carlos J. Bosques, Brian Edward Collins, Maurice Gaston Hains, Nathaniel J. Washburn, James Meador, Sean Smith, Naveen Bhatnagar
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Publication number: 20140115731Abstract: The invention relates to methods for the screening, identification and/or application of microorganisms of use in imparting beneficial properties to plants. In one embodiment, the method involves subjecting one or more plants to a growth medium in the presence of a set of microorganisms, selecting one or more plant, acquiring one or more microorganisms associated with the selected plant(s) and repeating the process one or more times. The method further involves the step of subjecting the one or more microorganisms to a conditioning and/or directed evolution process.Type: ApplicationFiled: October 10, 2013Publication date: April 24, 2014Applicant: Biodiscovery New Zealand LimitedInventors: Susan Jane Turner, Peter John Wigley
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Publication number: 20140112914Abstract: By replacing the antigen-binding domain, the present inventors discovered novel polypeptide complexes that retain BiTE's strong anti-tumor activity and excellent safety properties, as well as have long half-life in blood and can damage various different target cells.Type: ApplicationFiled: November 30, 2011Publication date: April 24, 2014Applicant: CHUGAI SEIYAKU KABUSHIKI KAISHAInventors: Junichi Nezu, Takahiro Ishiguro, Atsushi Narita, Akihisa Sakamoto, Yumiko Kawai, Tomoyuki Igawa, Taichi Kuramochi
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Publication number: 20140113352Abstract: The present invention provides compositions and methods for the expression of recombinant ?-glucosidase variants, as well as their use in the production of fermentable sugars from cellulosic biomass.Type: ApplicationFiled: December 19, 2013Publication date: April 24, 2014Applicant: Codexis, Inc.Inventors: Dipnath Baidyaroy, Louis Clark, Lisa M. Newman
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Publication number: 20140115740Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 13, 2013Publication date: April 24, 2014Applicants: Novozymes, Inc., Novozymes A/SInventors: Lan Tang, Ye Liu, Junxin Duan, Yu Zhang, Christian Isak Jorgensen, Randall Kramer
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Publication number: 20140115739Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: October 11, 2013Publication date: April 24, 2014Inventors: Suchindra Maiyuran, Randall Kramer, Paul Harris
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Publication number: 20140113338Abstract: The invention relates to an alpha-ketopimelic acid decarboxylase enzyme that is a homologue of SEQ ID NO:2, comprising at least one mutation selected from a group of substitutions listed in the specification, to a method for preparing 5-formyl valeric acid (hereinafter also referred to as ‘5-FVA’), to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’), to a method for preparing ?-caprolactam (hereinafter referred to as ‘caprolactam’) from 6-ACA, to a method for the preparation of adipic acid, to a method for preparing diaminohexane. The invention further relates to a host cell which may be used in a method according to the invention and to a polynucleotide encoding an alpha-ketopimelic acid decarboxylase enzyme.Type: ApplicationFiled: March 30, 2012Publication date: April 24, 2014Applicant: DSM IP ASSETS B.V.Inventors: Axel Christoph Trefzer, Stefanus Cornelis Hendrikus Josef Turk, Jan Metske Van Der Laan, Iise De Lange, Denise Ilse Jacobs
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Publication number: 20140113966Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.Type: ApplicationFiled: October 22, 2012Publication date: April 24, 2014Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: ROBERT DICOSIMO, MARK SCOTT PAYNE, JOHN EDWARD GAVAGAN
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Publication number: 20140113336Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: August 1, 2012Publication date: April 24, 2014Inventor: Nikolaj Spodsberg
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Patent number: 8703444Abstract: The present invention relates to micro-organisms and to methods of producing proteins. More specifically, the inventions relates to a host cell useful for the expression of heterozygous proteins in which the host cell, Pichia pastoris, has been genetically modified so that the gene for endogenous secreted subtilisin Sub2 protease is completely or partially inactivated.Type: GrantFiled: December 13, 2010Date of Patent: April 22, 2014Assignee: Centre Hospitalier Universitaire Vaudois (CHUV)Inventor: Michel Monod
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Publication number: 20140105889Abstract: The present invention demonstrated that the modification of the Fc region of an antigen-binding molecule into an Fc region that does not form in a neutral pH range a heterotetramer complex containing two molecules of FcRn and an active Fc? receptor improved the pharmacokinetics of the antigen-binding molecule and reduced the immune response to the antigen-binding molecule. The present invention also revealed methods for producing antigen-binding molecules having the properties described above, and successfully demonstrated that pharmaceutical compositions containing as an active ingredient such an antigen-binding molecule or an antigen-binding molecule produced by a production method of the present invention have excellent features over conventional antigen-binding molecules in that when administered, they exhibit improved pharmacokinetics and reduced in vivo immune response.Type: ApplicationFiled: March 30, 2012Publication date: April 17, 2014Applicant: CHUGAI SEIYAKU KABUSHIKI KAISHAInventors: Tomoyuki Igawa, Atsuhiko Maeda, Kenta Haraya, Yuki Iwayanagi, Tatsuhiko Tachibana, Futa Mimoto, Taichi Kuramochi, Hitoshi Katada, Shojiro Kadono
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Publication number: 20140106410Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 20, 2013Publication date: April 17, 2014Applicant: NOVOZYMES, INC.Inventor: Nikolaj Spodsberg
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Publication number: 20140106457Abstract: The present invention relates to a method for constructing a host cell expressing a polypeptide of interest from at least two ORF's stably integrated onto the chromosome of the host cell comprising the steps of: a) providing the at least two ORF's encoding the same polypeptide, wherein the at least two DNA sequences of the ORF's differ in at least one position; b) integrating the at least two ORF's in the same orientation on the host cell chromosome.Type: ApplicationFiled: September 23, 2013Publication date: April 17, 2014Inventors: Steen Troels Jorgensen, Poul Erik Pedersen
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Publication number: 20140105888Abstract: Novel modulators, including antibodies and derivatives thereof, and methods of using such modulators to treat hyperproliferative disorders are provided.Type: ApplicationFiled: February 17, 2012Publication date: April 17, 2014Applicant: Stem CentRx, Inc.Inventors: Orit Foord, Scott J. Dylla, Robert A. Stull, Alex Bankovich, Alexandra Leida Liana Lazetic, Jeffrey Bernstein
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Publication number: 20140105855Abstract: IL-17 Receptor binding proteins, including non-naturally occurring and recombinantly modified proteins, methods of making and using such molecules as therapeutic, prophylactic and diagnostic agents are provided.Type: ApplicationFiled: April 6, 2012Publication date: April 17, 2014Inventor: Kenan Christopher Garcia
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Patent number: 8697394Abstract: The present invention relates to eukaryotic host cells, especially lower eukaryotic host cells, having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar and sugar nucleotide transporters to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells exhibit GnTIII, GnTIV, GnTV, GnT VI or GnTIX activity, which produce bisected and/or multiantennary N-glycan structures and may be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar, sugar nucleotide transporters, to yield human-like glycoproteins.Type: GrantFiled: February 20, 2004Date of Patent: April 15, 2014Assignee: Glycofi, Inc.Inventors: Piotr Bobrowicz, Stephen R. Hamilton, Tillman U. Gerngross, Stefan Wildt, Byung-Kwon Choi, Juergen Hermann Nett, Robert C. Davidson
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Publication number: 20140096439Abstract: Provided herein are recombinant yeast host cells and methods for their use for production of fermentation products from a pyruvate utilizing pathway. Yeast host cells provided herein comprise reduced pyruvate decarboxylase activity and modified adenylate cyclase activity. In embodiments, yeast host cells provided herein comprise resistance to butanol and increased biomass production.Type: ApplicationFiled: October 3, 2013Publication date: April 10, 2014Inventors: MICHAEL G. BRAMUCCI, VASANTHA NAGARAJAN
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Publication number: 20140099670Abstract: Chimeric molecules comprising receptor activator of NF-?B ligand (RANKL) antibodies and parathyroid hormone/parathyroid hormone-related protein (PTH/PTHrP) peptides are described. Compositions and methods for the treatment of bone diseases are also described.Type: ApplicationFiled: March 15, 2013Publication date: April 10, 2014Applicant: AMGEN INC.Inventors: Paul KOSTENUIK, Wenyan SHEN, Thomas C. BOONE
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Publication number: 20140101785Abstract: Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided. In addition, methods of using such nucleic acids and polypeptides in methods of screening for agonists or antagonists of G-protein coupled receptor (GPCR) or ion channels and methods of monitoring neural activity also are provided.Type: ApplicationFiled: March 13, 2013Publication date: April 10, 2014Applicant: HOWARD HUGHES MEDICAL INSTITUTEInventors: Loren Looger, Karel Svoboda, Douglas Kim, Rex Kerr
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Publication number: 20140099678Abstract: Novel fungal endoglucanases Cel5 and Cel12 are disclosed. The endoglucanases are conveniently produced by recombinant technology, and means for their production are described. The endoglucanases are used for treating cellulosic material, especially in textile industry, e.g. in biofinishing or biostoning. They may also be used in detergents, in animal feed and/or in pulp and paper industry, or in hydrolysis of lignocellulosic material for, e.g. bioethanol production.Type: ApplicationFiled: October 18, 2013Publication date: April 10, 2014Applicant: AB Enzymes OyInventors: Terhi PURANEN, Leena VALTAKARI, Marika ALAPURANEN, George SZAKACS, Pentti OJAPALO, Jari VEHMAANPERA
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Publication number: 20140101793Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: December 12, 2013Publication date: April 10, 2014Applicants: Novoyzmes A/S, Novozymes, Inc.Inventor: Marc Dominique Morant
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Patent number: 8691960Abstract: Described herein are compositions and methods for generating oxidoreductases for enantioselective reactions. Described herein are compositions and methods for generating neomorphic (R)-2-hydroxyacid dehydrogenases capable of enzymatically converting a 1-carboxy-2-ketoacid to a 1-carboxy-(R)-2-hydroxyacid, or the reverse reaction. Illustrative examples include (a) (R)-2-hydroxyadipate dehydrogenase and uses thereof for converting 2-oxoadipate to (R)-2-hydroxyadipate, or the reverse reaction; and (b) (R)-2-hydroxyglutarate dehydrogenase and uses thereof for converting 2-oxoglutarate to (R)-2-hydroxyglutarate, or the reverse reaction. Also described herein are compositions and methods for generating non-natural microbial organisms to enzymatically convert 2-oxoadipate to (E)-2-hexenedioate or adipate, or to enzymatically convert 2-oxoglutarate to (E)-2-pentenedioate or glutarate, or the respective reverse reactions.Type: GrantFiled: February 27, 2013Date of Patent: April 8, 2014Assignee: Duke UniversityInventors: Zachary James Reitman, Hai Yan, Bryan Daehahn Choi, John Howard Sampson