Rhizopus Patents (Class 435/254.9)
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Publication number: 20120107905Abstract: The present invention relates to fungal serine protease variants, which comprise an amino acid substitution of valine at position 208 of the parent Fusarium equiseti Fe_RF6318 serine protease, wherein the position of the substitution corresponds to the amino acid sequence of the mature Fe_RF6318 enzyme defined in SEQ ID NO:2. The variants have improved thermal stability and/or detergent stability compared to the parent Fe_RF6318 enzyme. Preferably the substitution is V208I and more preferably the variants comprise additional amino acid changes which further increase the stability. Also disclosed are nucleic acid sequences encoding said protease variants as well as recombinant vectors and host cells for the production of the variants.Type: ApplicationFiled: October 28, 2011Publication date: May 3, 2012Applicant: AB ENZYMES OYInventors: Kari Juntunen, Leena Valtakari, Nina Hakulinen, Marja Paloheimo
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Publication number: 20120040426Abstract: The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.Type: ApplicationFiled: June 24, 2011Publication date: February 16, 2012Applicant: GENOMATICA, INC.Inventors: Jun Sun, Priti Pharkya, Anthony P. Burgard
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Publication number: 20120034648Abstract: The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least about 70% sequence identity to the amino acid sequence set out in SEQ ID NO: 3 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.Type: ApplicationFiled: March 5, 2009Publication date: February 9, 2012Inventors: Paul Klaassen, Jan Metske Van Der Laan, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom
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Publication number: 20120015408Abstract: The invention provides variants of the Thermoanaerobacter brockii CglT beta-glucosidase that have improve beta-glucosidase activity compared to the wild type enzyme. The invention also provides polynucleotides that encode the variants, as well as methods of producing the variants, enzyme compositions comprising the variants, and methods for using the variants in industrial applications.Type: ApplicationFiled: February 26, 2010Publication date: January 19, 2012Applicant: CODEXIS, INC.Inventors: Dipnath Baidyaroy, Louis Clark, Lisa M. Newman, Charlene Ching
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Publication number: 20120003703Abstract: The invention relates to recombinant expression of variant forms of C1 CBH1a and homologs thereof, having improved thermostability, low-pH tolerance, specific activity and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.Type: ApplicationFiled: May 13, 2011Publication date: January 5, 2012Applicant: Codexis, Inc.Inventors: Vesna Mitchell, Grzegorz Wojciechowski, Oscar Alvizo
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Publication number: 20120003708Abstract: Methods of producing C4 dicarboxylic acids are disclosed. Nucleotide sequences encoding pyruvate carboxylase and uses of such nucleotide sequences in the production of C4 dicarboxylic acids are further disclosed.Type: ApplicationFiled: March 23, 2010Publication date: January 5, 2012Applicant: ARCHER DANIELS MIDLAND COMPANYInventors: Beth Fatland-Bloom, John P. Rayapati
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Publication number: 20110318795Abstract: The present invention relates to a recombinant fungus comprising an enzyme which catalyses the conversion of malic acid to fumaric acid in the cytosol. The invention further relates to a process for the production of a dicarboxylic acid such as fumaric acid and succinic acid, wherein the recombinant fungus is used.Type: ApplicationFiled: November 14, 2008Publication date: December 29, 2011Inventors: René Verwaal, Liang Wu, Robbertus Antonius Damveld, Cornelis Maria Jacobus Sagt
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Publication number: 20110312046Abstract: The present invention relates to methods of producing C4-dicarboxylic acids, such as malic acid, comprising: (a) cultivating a host cell comprising a polynucleotide encoding a C4-dicarboxylic acid transporter; and (b) recovering the C4-dicarboxylic acid. The present invention also relates to methods for increasing C4-dicarboxylic acid production, as well as host cells comprising the polynucleotides.Type: ApplicationFiled: June 21, 2011Publication date: December 22, 2011Applicant: NOVOZYMES, INC.Inventors: SHERYL LUTTRINGER, DEBBIE YAVER
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Publication number: 20110312049Abstract: The invention provides non-naturally occurring microbial organisms having an ethylene glycol pathway. The invention additionally provides methods of using such organisms to produce ethylene glycol.Type: ApplicationFiled: April 13, 2011Publication date: December 22, 2011Inventors: Robin E. Osterhout, Priti Pharkya, Anthony P. Burgard
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Publication number: 20110300597Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.Type: ApplicationFiled: May 4, 2011Publication date: December 8, 2011Inventors: Mark J. Burk, Anthony P. Burgard, Jun Sun, Robin E. Osterhout, Priti Pharkya
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Publication number: 20110294191Abstract: Described are compositions and methods relating to filamentous fungal cells genetically engineered to provide increased production of aspartic proteases, such as PEPAa, PEPAb, PEPAc, and PEPAd. Also described are nucleic acids and methods for making the engineered filamentous fungal cells.Type: ApplicationFiled: July 28, 2009Publication date: December 1, 2011Applicant: DANISCO US INC.Inventor: Huaming Wang
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Publication number: 20110275116Abstract: Genetically manipulated cells, lysates of such cells, systems, and methods of use thereof are provided, where one or more enzymes in a pathway of interest are genetically modified to incorporate a peptide sequence that provides for relocation of the protein, e.g., to the periplasm, so as to sequester the enzyme, and where the enzyme controls flux in the pathway of interest.Type: ApplicationFiled: May 6, 2011Publication date: November 10, 2011Inventor: James R. Swartz
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Publication number: 20110277043Abstract: The present invention relates to polynucleotides from Cochliobolus heterostrophus C5, Cyanothece sp. CCY0110, Mycocentrospora acerina and Hyaloperonospora parasitica, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.Type: ApplicationFiled: December 8, 2009Publication date: November 10, 2011Applicant: BASF Plant Science Company GmbHInventors: Toralf Senger, Jörg Bauer, Johnathan A. Napier
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Publication number: 20110262619Abstract: The invention relates to a recombinant DNA molecule coding a polypeptide having phytase stability after being expressed in a prokaryotic or eukaryotic host cell. Said recombinant DNA molecule comprises a DNA sequence selected from among a) DNA sequences coding a polypeptide that has phytase activity and is obtained by varying the mature wild-type E. coli phytase sequence, the variation being selected from among i) the mutation lysine?aspartic acid in position 74 (K74D), and/or ii) a combination of the mutations asparagine?arginine in position 139 (N139R) and aspartic acid?glutamic acid in position 142 (D142E), and/or iii) a combination of the mutations leucine?isoleucine in position 145 (L145I) and leucine?isoleucine in position 198 (L198I), and/or iv) a mutation valine?proline in position 200 (V200P), and/or v) an N-terminal or C-terminal or an N-terminal and C-terminal addition of a sequence section of the acidic phosphatase of Aspergillus niger var.Type: ApplicationFiled: November 2, 2007Publication date: October 27, 2011Applicant: AB Enzymes GmbHInventors: Khanh Q Nguyen, Bruno Winter
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Publication number: 20110189728Abstract: The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least about 70% sequence identity to the amino acid sequence set out in SEQ ID NO: 3 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.Type: ApplicationFiled: March 5, 2009Publication date: August 4, 2011Inventors: Paul Klaassen, Jan Metske Laan, Van Der, Bianca Eiisabeth Maria Gielesen
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Patent number: 7977084Abstract: The invention provides a non-naturally occurring microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.Type: GrantFiled: March 5, 2009Date of Patent: July 12, 2011Assignee: Genomatica, Inc.Inventors: Jun Sun, Priti Pharkya, Anthony P. Burgard
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Publication number: 20110159544Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used, for example for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology are described, as well as uses of the enzymes and enzyme preparations.Type: ApplicationFiled: December 30, 2010Publication date: June 30, 2011Applicant: ROAL OyInventors: Terhi Puranen, Sauli Toikka, Kim Langfelder, Jari Vehmaanpera
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Publication number: 20110086133Abstract: The invention relates to the use of one or more pectinolytic enzyme(s) for the treatment of fruit or vegetable mash as well as a process for enzymatic treatment of fruit or vegetable mash comprising the step of adding one or more pectinolytic enzyme(s), wherein at least one pectinolytic enzyme is obtainable from Trichoderma reesei, as well as to a process for the preparation of a fruit or vegetable juice comprising the process for enzymatic treatment of fruit or vegetable mash. Moreover, the invention discloses recombinant DNA molecules encoding a polypeptide having endo-polygalacturonase activity, a polypeptide having exo-polygalacturonase activity, a polypeptide having exo-rhamnogalacturonase activity and a polypeptide having xylogalacturonase activity.Type: ApplicationFiled: May 22, 2009Publication date: April 14, 2011Applicant: AB ENZYMES GMBHInventors: Terhi Puranen, Bernhard Seiboth, Klaudija Milos, Wilfried Theiss, Jarno Kallio, Christian Kubicek, Jari Vehmaanperä
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Publication number: 20110053233Abstract: The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.Type: ApplicationFiled: August 27, 2010Publication date: March 3, 2011Applicant: NOVOZYMES, INC.Inventors: STEPHEN BROWN, SHERYL LUTTRINGER, DEBBIE YAVER, ALAN BERRY
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Publication number: 20110053195Abstract: An inactive xylanase molecule for the recovery of xylanase activity in plant-derived material containing active xylanase enzyme(s) and xylanase inhibitors. The inactive xylanase molecule of binds to xylanase inhibitors in the plant-derived material, thereby allowing accurate measurement of xylanase enzyme activity of the enzyme contained in the plant-derived material. The invention further includes amino acid molecules depicted by SEQ ID NOS. 4 through 112, wherein the catalytically active sites of each of the amino acids have been modified resulting in inactive xylanase molecules. A method of production of the inactive xylanase molecules includes expression of the inactive xylanase molecule in microbial or eukaryal (e.g.Type: ApplicationFiled: June 12, 2007Publication date: March 3, 2011Applicant: SYNGENTA PARTICIPATIONS AGInventors: Michael William Bauer, Justin DeFontes
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Publication number: 20110028375Abstract: The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence the mature Fa_RF7182 enzyme having an amino acid sequence of SEQ ID NO: 18. The serine protease is obtainable from Fusarium acuminatum, more preferably from the deposited strain CBS 124084. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2530 comprising the nucleotide sequence SEQ ID NO:12 deposited in Escherichia coli RF7803 under accession number DSM 22208 and plasmid pALK2531 comprising the full-length gene SEQ ID NO: 13 deposited in E. coli RF7879 under accession number DSM 22209, as well as fungal hosts, such as Trichoderm. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.Type: ApplicationFiled: April 28, 2010Publication date: February 3, 2011Applicant: AB Enzymes OyInventors: Kari Juntunen, Leena Valtakari, Susanna Makinen, Jarno Kallio, Jari Vehmaanpera, Pentti Ojapalo, Marja Paloheimo, George Szakacs
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Publication number: 20110008870Abstract: The present invention is related to a fungal serine protease enzyme useful in modification, degradation or removal of proteinaceous material, which enzyme comprises an amino acid sequence of the mature Tr Prb1 enzyme having an amino acid sequence of SEQ ID NO: 10 or a variant thereof having similar activity. The serine protease is obtainable from Trichoderma. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2650 comprising the nucleotide sequence SEQ ID NO: 10 of the full length enzyme deposited in E. coli RF8052 under accession number DSM 22635. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material at low or moderate temperature ranges.Type: ApplicationFiled: June 28, 2010Publication date: January 13, 2011Applicant: AB Enzymes OyInventors: Susanna Makinen, Pentti Ojapalo, Leena Valtakari, Kari Juntunen, Marja Paloheimo
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Publication number: 20100323426Abstract: The present invention provides filamentous fungi that express a combination of heterologous and homologous polypeptides, polypeptide mixtures comprising a combination of heterologous and homologous polypeptides and methods of producing the polypeptide mixtures.Type: ApplicationFiled: June 5, 2008Publication date: December 23, 2010Inventors: Benjamin S. Bower, Edmund A. Larenas
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Publication number: 20100323414Abstract: The invention provides methods of producing lipids by oleaginous microorganisms. The invention also provides genetically engineered oleaginous microorganisms and methods of cultivation for lipid production. Also provided are oils, fuels, oleochemicals, chemical precursors, and other compounds manufactured such microorganisms. Exemplary oleaginous microorganisms include oleaginous fungi and such oleaginous fungi that have been genetically modified.Type: ApplicationFiled: April 30, 2010Publication date: December 23, 2010Applicant: Solazyme, Inc.Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
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Patent number: 7838278Abstract: Nucleotide and protein sequences that encode enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as a R. oryzae cell, are provided. Methods of manipulating carbon flux in a cell also are provided.Type: GrantFiled: March 23, 2009Date of Patent: November 23, 2010Assignee: Archer-Daniels-Midland CompanyInventors: Beth Fatland-Bloom, P. John Rayapati, Nyerhovwo John Tonukari
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Publication number: 20100143989Abstract: This invention relates to nitrilase mutants having improved nitrilase activity for converting 3-hydroxynitriles to 3-hydroxycarboxylic acids. More specifically, the Acidavorax facilis 72W (ATCC 55746) nitrilase gene was mutated using error-prone PCR and site-directed mutagenesis to create nitrilase enzymes having improved nitrilase activity for converting 3-hydroxynitriles (e.g., 3-hydroxybutyronitrile or 3-hydroxyvaleronitrile) to the corresponding 3-hydroxycarboxylic acids. A process using these improved mutants to produce the 3-hydroxycarboxylic acids is also provided.Type: ApplicationFiled: April 14, 2008Publication date: June 10, 2010Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: Mark S. Payne, Robert DiCosimo, Daniel P. O'Keefe
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Publication number: 20100119656Abstract: The invention relates to a DNA sequence that encodes a polypeptide with lysophospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with lysophospholipase activity encoded by these sequences as well as the use of these polypeptides for improving the filterability of syrups consisting of wheat starch and for related applications.Type: ApplicationFiled: September 21, 2007Publication date: May 13, 2010Applicant: AB Enzymes GmbHInventors: Khanh Q. Nguyen, Volker Marschner, Kornelia Titze, Bruno Winter
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Publication number: 20100112651Abstract: Nucleotide and protein sequences that encode enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as a R. oryzae cell, are provided. Methods of manipulating carbon flux in a cell also are provided.Type: ApplicationFiled: March 23, 2009Publication date: May 6, 2010Applicant: Archer-Daniels-Midland CompanyInventors: Beth Fatland-Bloom, P. John Rayapati, Nyerhovwo John Tonukari
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Publication number: 20100009419Abstract: A non-naturally occurring eukaryotic or prokaryotic organism includes one or more gene disruptions occurring in genes encoding enzymes imparting increased fumarate, malate or acrylate production in the organism when the gene disruption reduces an activity of the enzyme. The one or more gene disruptions confers increased production of acrylate onto the organism. Organisms that produce acrylate have an acrylate pathway that at least one exogenous nucleic acid encoding an acrylate pathway enzyme expressed in a sufficient amount to produce acrylate, the acrylate pathway comprising a decarboxylase. Methods of producing fumarate, malate or acrylate include culturing these organisms.Type: ApplicationFiled: June 17, 2009Publication date: January 14, 2010Inventors: Mark J. Burk, Anthony P. Burgard, Priti Pharkya
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Publication number: 20090275097Abstract: The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.Type: ApplicationFiled: March 5, 2009Publication date: November 5, 2009Inventors: Jun Sun, Priti Pharkya, Anthony P. Burgard
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Publication number: 20090253173Abstract: The invention relates to a filamentous fungal cell (e.g., Aspergillus sp.) comprising at least one inactivated protease gene chosen from apsB, a homolog of apsB, cpsA, a homolog cpsA, and combinations thereof. Nucleic acids and methods for making the inactivated mutant filamentous fungal cells are provided as well as methods for using the cells for the altered production of endogenous or heterologous proteins of interest.Type: ApplicationFiled: March 26, 2009Publication date: October 8, 2009Applicant: Danisco US Inc., Genencor DivisionInventor: Huaming Wang
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Patent number: 7566563Abstract: Nucleotide sequences and genetic constructs that can be used to regulate genes encoding enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as a R. oryzae cell, are provided. Methods of manipulating carbon flux in a cell also are provided.Type: GrantFiled: August 21, 2008Date of Patent: July 28, 2009Assignee: Archer-Daniels-Midland CompanyInventors: Beth Fatland-Bloom, Gyan Rai, P. John Rayapati, Nyerhovwo John Tonukari
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Publication number: 20090169536Abstract: The invention relates to a recombinant DNA molecule, which, upon expression in a prokaryotic or eukaryotic host cell, encodes a polypeptide having phytase activity, wherein the recombinant DNA molecule comprises a DNA sequence selected from a) DNA sequences that have been obtained by variations of the mature wild-type E. coli phytase sequence, wherein at least one amino acid in the region of position 189 to 211 and/or an amino acid in the region of position 137 to 152 is mutated as compared to the wild-type sequence, b) DNA sequences having a homology of 70% to 100% to the sequences according to a), c) DNA sequences that are related to the sequences according to a) and b) due to the degeneracy of the genetic code, wherein the recombinant DNA molecule is, upon expression in a suitable host cell, associated with an increased activity of the thus encoded protein in the culture supernatant, as well as the proteins encoded by the same.Type: ApplicationFiled: October 14, 2005Publication date: July 2, 2009Applicant: AB ENZYMES GMBHInventors: Khanh Q. Nguyen, Bruno Winter
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Publication number: 20090061493Abstract: The invention provides methods of modifying the lipids produced by microbial organisms through genetic engineering. The invention also provides genetically engineered microbes and methods of fermenting microbes for oil production. Also provided are oils, fuels, oleochemicals, chemical precursors, and other compounds manufactured by such modified microorganisms. Exemplary oil-bearing organisms include organisms containing one or more exogenous genes encoding a fatty acyl-ACP thioesterase, fatty acyl-CoA/aldehyde reductase, fatty acyl-CoA reductase, fatty aldehyde reductase, fatty aldehyde decarbonylase, and/or an acyl carrier protein.Type: ApplicationFiled: June 2, 2008Publication date: March 5, 2009Applicant: SOLAZYME, INC.Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
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Patent number: 7491510Abstract: The present invention relates to the use of a glucosidase II mutation to increase protein secretion in eukaryotic cells. The present invention relates further to the use of eukaryotic cells, comprising a mutant glucosidase II gene, possibly in combination with the expression of a recombinant ?-1,2-mannosidase gene and/or a recombinant N-acetylglucosaminyl-transferase gene, as a host for protein secretion.Type: GrantFiled: September 12, 2005Date of Patent: February 17, 2009Assignees: Vlaams Interuniversitair Institute voor Biotechnologie vzw, Universiteit GentInventors: Roland Contreras, Steven Geysens
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Publication number: 20090042264Abstract: Nucleotide sequences and genetic constructs that can be used to regulate genes encoding enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as a R. oryzae cell, are provided. Methods of manipulating carbon flux in a cell also are provided.Type: ApplicationFiled: August 21, 2008Publication date: February 12, 2009Applicant: Archer-Daniels-Midland CompanyInventors: Beth Fatland-Bloom, Gyan Rai, P. John Rayapati, Nyerhovwo John Tonukari
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Publication number: 20090035842Abstract: The invention provides methods of manufacturing oils and oil-based products such as transportation fuels, industrial chemicals, edible oils, lubricants and plastics using sugar cane, sugar beets, and cane/beet agricultural processing byproducts as a feedstock for bioproduction processes. The disclosed processes utilize oil-bearing microbes as a conversion technology to convert chemical energy produced by sugar cane and sugar beets into energy-containing oils and oil derivatives. Also provided herein are oil-bearing microbes containing one or more exogenous sucrose utilization genes.Type: ApplicationFiled: June 2, 2008Publication date: February 5, 2009Applicant: SOLAZYME, INC.Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
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Patent number: 7435168Abstract: Nucleotide sequences and genetic constructs that can be used to regulate genes encoding enzymes that change carbon flux through metabolic pathways that lead to lactic acid or fumarate production in a host cell, such as a R. oryzae cell, are provided. Methods of manipulating carbon flux in a cell also are provided.Type: GrantFiled: January 17, 2006Date of Patent: October 14, 2008Assignee: Archer-Daniels-Midland CompanyInventors: Beth Fatland-Bloom, Gyan Rai, P. John Rayapati, Nyerhovwo John Tonukari
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Patent number: 7432089Abstract: The present invention relates to a DNA encoding a novel flavin adenine dinucleotide (FAD)-dependent D-erythronate 4-phosphate (EN4P) dehydrogenase originated from Sinorhizobium meliloti, which is involved in vitamin B6 biosynthesis, and a recombinant microorganism transformed with a vector having the DNA. It also relates to a process for production of vitamin B6 by using the recombinant microorganism. “Vitamin B6” as used in this invention includes pyridoxol (PN), pyridoxal and pyridoxamine. Vitamin B6 is a vitamin indispensable to human beings or other animals, and is used as a raw material of medicines or as feed additives.Type: GrantFiled: September 25, 2003Date of Patent: October 7, 2008Assignee: DSM IP Assets B.V.Inventors: Tatsuo Hoshino, Keiko Ichikawa, Masaaki Tazoe
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Patent number: 7291493Abstract: The present invention relates to codon-optimized xylanase coding sequences and the expression of xylanases in microbes and yeast. The invention further relates to using multiple copies of the xylanase expression construct for high levels of protein expression. The invention also relates to the use of xylanases as feed or food additives. The invention also relates to methods of expression of enzymes to increase thermotolerance by expressing them in organisms that glycosylate proteins compared to expression that the same enzyme without the glycosylation. Further, the invention relates to methods of preparing feed, enzyme feed additives, and methods of reducing the feed conversion ration or increasing weight gain of animals.Type: GrantFiled: December 20, 2004Date of Patent: November 6, 2007Assignee: Syngenta Participations AGInventors: Michael Bauer, Michael Richard Bedford, Derrick Allen Pulliam
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Patent number: 6849444Abstract: Disclosed are novel strains of Rhizopus oryzae and uses thereof. The strains of the invention are temperature-resistant and convert a carbon source to lactic acid at high temperatures.Type: GrantFiled: April 23, 2002Date of Patent: February 1, 2005Assignee: Archer Daniels Midland CompanyInventor: Hungming J. Liaw
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Patent number: 6436643Abstract: A process for site-directed integration of multiple copies of a gene in a mould is provided, which comprises transforming a mould cell containing in its chromosomal DNA a restriction site for a rare-cutting endonuclease, e.g., I-Scel, preferably introduced at a desired locus, e.g., within a selectable marker gene or in the neighborhood thereof, with a piece of DNA comprising multiple copies of at least one expressible gene comprising at least one structural gene encoding a desired protein, surrounded by two DNA fragments homologous to part of the DNA upstream and downstream, and in the neighborhood, of said restriction site, while during the transformation of the mould the presence of the rare-cutting endonuclease is provided, followed by selecting or screening for a mould cell in which the multiple gene copies of said expressible gene are inserted into the chromosomal DNA of the mould.Type: GrantFiled: August 2, 2000Date of Patent: August 20, 2002Assignee: Unilever Patent Holdings BVInventors: Marcellus Johannes Augustinus de Groot, Alida Godelieve Maria Beijersbergen, Wouter Musters
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Patent number: 6432672Abstract: The present invention relates to filamentous fungi that comprise in their genomes at least two substantially homologous DNA domains which are suitable for integration of one or more copies of a recombinant DNA molecule and wherein at least two of these DNA domains comprise an integrated copy of a recombinant DNA molecule. The invention also relates to methods for preparing such filamentous fungi and for further multiplying the DNA domains with integrated recombinant DNA molecules through gene conversion or amplification.Type: GrantFiled: January 24, 2000Date of Patent: August 13, 2002Inventors: Gerardus Cornelis Maria Selten, Bart Willem Swinkels, Roelof Ary Lans Bovenberg
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Patent number: 6399338Abstract: The invention relates to a method of producing chitin or chitosan by culturing a Rhizopus azygosporus fungus or an Actinomucor taiwanensis fungus and isolating chitosan or chitin from the culture.Type: GrantFiled: September 27, 2000Date of Patent: June 4, 2002Assignee: Food Industry Research and Development InstituteInventors: Mei-Huei Chen, Hing-Yuen Chan, Chih-Lu Wu, Su-Hui Chuang, Ing-Er Hwang, Yen-Lin Chen, Gwo-Fang Yuan