Candida Or Torulopsis Patents (Class 435/255.4)
-
Patent number: 10287615Abstract: The present invention relates to a process for selectively producing sophorolactone without use of organic solvent, comprising the steps of: —pre-cultivating cells of a Candida species capable of producing sophorolactone, in absence of an oily substrate until a stationary growth phase is obtained, —cultivating said pre-cultivated cells in an aqueous medium in the presence of at least one fermentable sugar and substrate; the reaction mixture of sugar, substrate and pre-cultivated cells being present in an amount and conditions such that the cells metabolize the sugar and substrate thereby forming sophorolactone and fatty acid, —continuously feeding said substrate to said cells thereby suppressing the formation of fatty acid and keeping fatty acid levels in the reaction mixture below 10 g/l, resulting in the crystallization of at least part of the sophorolactone present in the reaction mixture, —warming the reaction mixture to a temperature between 60° C. and 90° C.Type: GrantFiled: June 6, 2012Date of Patent: May 14, 2019Assignee: ECOVER CO-ORDINATION CENTER N.V.Inventors: Dirk Develter, Steve Fleurackers
-
Patent number: 10106790Abstract: Intergenic non-coding RNA molecules that regulate the expression of HWP1 and ALS3 of Candida are provided as are methods of using the non-coding RNA molecules and complementary molecules thereof in modulating HWP1 or ALS3 expression; adherence, yeast-to-hyphal transition, or biofilm development of Candida; and preventing or treating candidiasis.Type: GrantFiled: September 18, 2015Date of Patent: October 23, 2018Assignee: TRUSTEES OF DARTMOUTH COLLEGEInventors: Paula R. Sundstrom, Samin Kim, Quoc Bao Nguyen
-
Publication number: 20150132740Abstract: A concentration agent for microorganisms is provided that contains both lanthanum and carbonate. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided.Type: ApplicationFiled: May 23, 2013Publication date: May 14, 2015Inventors: Manjiri T. Kshirsagar, Evan Koon Lun Yuuji Hajime, Andrew W. Rabins
-
Patent number: 9005683Abstract: Provided is a method for producing a yeast containing glutamic acid at a high concentration. In the method for culturing a yeast, a yeast in a stationary growth phase is subjected to liquid culture under the conditions that the pH of a liquid medium is 7.5 or higher and lower than 11. After the pH of the liquid medium for the yeast in a stationary growth phase is adjusted to 7.5 or higher and lower than 11, the yeast is further cultured, whereby a yeast with a high glutamic acid content can be produced. In the invention, as the yeast, Saccharomyces cerevisiae or Candida utilis can be used. Therefore, by using the yeast with a high glutamic acid content obtained by the above-said method and the extract obtained by extraction from the yeast, a seasoning composition and a food or drink with a high glutamic acid content can be provided.Type: GrantFiled: October 30, 2009Date of Patent: April 14, 2015Assignee: Asahi Group Holdings, Ltd.Inventors: Ichiro Shibuya, Hiroaki Okano, Yoshitomo Kanaoka, Nobuchika Takesue
-
Patent number: 8986956Abstract: The present invention relates to a method for producing hEGF (human epidermal growth factor) which has the same activity as the wild form, in high concentration and with a high degree of purity. More specifically, the invention relates to an hEGF expression vector comprising a nucleic acid sequence coding for the polypeptide of sequence number 14; a host cell in which the expression vector has been genetically transformed; and a method for producing hEGF, comprising a step in which the expression vector is created and is genetically transformed in yeast from which the KEX1 gene is lacking. Using the method of the present invention, it is possible to produce a large volume of human derived EGF which has the same size and activity as human derived EGF, and this EGF can be used in various ways such as in medicine and cosmetics.Type: GrantFiled: November 4, 2011Date of Patent: March 24, 2015Assignee: Korea Research Institute of Bioscience and BiotechnologyInventors: Jung Hoon Sohn, Jung Hoon Bae, Mi Jin Kim, Hyun Jin Kim, Soon Ho Park, Kwang Mook Lim
-
Publication number: 20150044739Abstract: A strain of Candida sake, CAT H430, is provided. The methods of using CAT H430 for producing dicarboxylic acids are also provided.Type: ApplicationFiled: March 9, 2012Publication date: February 12, 2015Applicants: CATHAY INDUSTRIAL BIOTECH LTD., CATHAY R&D CENTER CO., LTD.Inventors: Charlie Liu, Jinxiu Liao, Jianglin Wang, Haibin Qin, Naiqiang Li
-
Patent number: 8921082Abstract: A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeast Candida sp., xylose can be effectively converted into xylitol. The invention also provides the Candida strain having high furfural tolerance, and is capable to produce xylitol from various types of non-detoxified lignocellulosic hydrolysates, in which the overall utilization of xylose in hydrolysate can reach over 95%.Type: GrantFiled: March 1, 2013Date of Patent: December 30, 2014Assignee: Institute of Nuclear Energy Research Atomic Energy Council, Executive YuanInventors: Chiung-Fang Huang, Yi-Feng Jiang, Ting-Hsiang Lin, Gia-Luen Guo, Wen-Song Hwang, Jia-Baau Wang
-
Publication number: 20140329840Abstract: 7-(substituted) derivatives of 7H-pyrrolo[3,2-f]-quinazoline-1,3-diamines, derivative thereof, and methods of using them are provided. The pharmaceutical formulations prepared from the compounds can be used to treat a variety of conditions, which include, but are not limited to bacterial and fungal infections. The compounds can also be used as a sterilizing or disinfecting agent.Type: ApplicationFiled: May 5, 2014Publication date: November 6, 2014Applicant: Georgia Tech Research CorporationInventors: Bharath Srinivasan, Jeffrey Skolnick, Hongyi Zhou
-
Patent number: 8835152Abstract: Compositions of peptides and surface-active agents are described, as are methods of making and using such compositions. The compositions are capable of affecting metabolic rates in biological systems, and to accelerate nutrient uptake without a concomitant increase in biofilm production.Type: GrantFiled: December 4, 2012Date of Patent: September 16, 2014Assignee: Advanced Biocatalytics CorporationInventor: Carl W. Podella
-
Patent number: 8815566Abstract: Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 25% eicosapentaenoic acid (EPA, an ?-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.Type: GrantFiled: July 17, 2013Date of Patent: August 26, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Howard Glenn Damude, Peter John Gillies, Daniel Joseph Macool, Stephen K Picataggio, Dana M. Walters Pollak, James John Ragghianti, Zhixiong Xue, Narendra S Yadav, Hongxiang Zhang, Quinn Qun Zhu
-
Patent number: 8735338Abstract: Surfactant-containing compositions are described which include a protein component that has the effect of improving the surface-active properties of the surfactants contained in the compositions. The surfactant-containing compositions having the protein component demonstrate significantly lower critical micelle concentrations (CMC), reduced surface tensions, and reduced interfacial tensions than do comparable compositions having no protein component. In addition, the surfactant-containing compositions having the protein component has the effect of converting greasy waste contaminants to surface active materials.Type: GrantFiled: May 29, 2012Date of Patent: May 27, 2014Assignee: Advanced Biocatalytics Corp.Inventors: John W. Baldridge, Carl W. Podella
-
Patent number: 8703474Abstract: Disclosed are a novel flavor compound-producing yeast strain and methods of using the strain to produce flavor compounds.Type: GrantFiled: August 16, 2011Date of Patent: April 22, 2014Assignee: Academia SinicaInventors: Jui-Jen Chang, Cheng-Yu Ho, Chieh-Chen Huang, Ming-Che Shih, Wen-Hsiung Li
-
Publication number: 20140109249Abstract: The present invention relates to methods for the screening, identification and/or application of microorganisms of use in imparting beneficial properties to plants.Type: ApplicationFiled: October 10, 2013Publication date: April 17, 2014Applicant: Biodiscovery New Zealand LimitedInventors: Susan Jane Turner, Peter John Wigley
-
Patent number: 8691540Abstract: From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.Type: GrantFiled: May 24, 2012Date of Patent: April 8, 2014Assignee: Butamax Advanced Biofuels LLCInventors: Michael G. Bramucci, Andrew C. Eliot, Lori Ann Maggio-Hall, Charles E. Nakamura
-
Patent number: 8647840Abstract: The invention provides orthogonal translation systems for the production of polypeptides comprising unnatural amino acids in methylotrophic yeast such as Pichia pastoris. Methods for producing polypeptides comprising unnatural amino acids in methylotrophic yeast such as Pichia pastoris are also provided.Type: GrantFiled: December 10, 2008Date of Patent: February 11, 2014Assignee: The Scripps Research InstituteInventors: Travis Young, Peter G. Schultz
-
Publication number: 20140017767Abstract: The present invention relates to microbial inocula and in particular to standardised inocula for producing reference cultures of microorganisms. The invention has been developed primarily for use as a solid, disc-shaped, inoculum and will be described hereinafter with reference to this application. However, it will be appreciated that the invention is not limited to this particular field of use.Type: ApplicationFiled: June 21, 2013Publication date: January 16, 2014Applicant: AMS Laboratories Pty LtdInventors: Paul PRISCOTT, Yi-hong WANG
-
Patent number: 8623633Abstract: Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.Type: GrantFiled: May 14, 2011Date of Patent: January 7, 2014Assignee: Cargill IncorporatedInventors: Vineet Rajgarhia, Kari Koivuranta, Merja Penttilä, Marja Ilmen, Pirkko Suominen, Aristos Aristidou, Christopher Kenneth Miller, Stacey Olson, Laura Ruohonen
-
Patent number: 8617832Abstract: The present invention features a method for identifying an agent that inhibits Candida albicans-mediated differentiation of keratinocytes. Agents identified by the screening assay of the invention find application in the prevention and treatment of candidiasis.Type: GrantFiled: August 13, 2010Date of Patent: December 31, 2013Assignee: Trustees of Dartmouth CollegeInventor: Paula Sundstrom
-
Publication number: 20130344572Abstract: A hyperbranched polymer based on one or more repeating units of an ABx type monomer, wherein A and B are functional groups and x is greater than or equal to 2, wherein A reacts with, or substantially reacts with, B, wherein B is fractionally functionlized with a plurality of functional groups comprising a first functional group comprising a C6-C30 alkyl chain attached to the repeating unit through a carbonyl group (C?O) via an ester linkage, a second functional group comprising a partially fluorinated or perfluorinated C20 alkyl chain attached to the repeating unit through a carbonyl group (C?O) via an ester linkage, and a third functional group comprising substantially one of a stabilized radical source attached to the repeating unit via a C0-C6 tether, or a 5 to 8 member chloroamide heterocycle of carbon and nitrogen that is attached to the repeating unit via a C2-C6 tether.Type: ApplicationFiled: June 25, 2013Publication date: December 26, 2013Inventors: Andre A. Williams, Joshua A. Orlicki
-
Publication number: 20130288340Abstract: An object of the present invention is to provide a microorganism which is novel at the elemental composition level, and to provide a technique for providing such a microorganism. The present inventor has succeeded in causing a microorganism to efficiently contain a non-essential element by decreasing the content of an essential nutrient source for the microorganism, a C source, an N source, a P source, or an S source, and by adding an X compound containing the non-essential element as a constitutive element in a manner to make up for the decreased amount, and then culturing the microorganism.Type: ApplicationFiled: September 5, 2011Publication date: October 31, 2013Applicants: PARA MICROBIOS LABORATORIES LLCInventor: Yoshitake Tanaka
-
Patent number: 8530226Abstract: The invention relates to a yeast cell producing isobutanol, characterized in that the cell has an increased metabolic flow of material from pyruvate and acetolactate, 2,3-dihydroxy isovalerate, 2-ketoisovalerate, isobutyraldehyde to isobutanol, in that at least one of the genes coding the enzymes, which are involved in this conversion, is over-expressed, and without any of said genes being heterologous to said yeast cell, and to a method for the production of isobutanol using yeast cells, comprising the provision of the yeast cells according to the invention, and bringing the yeast cell into contact with a fermentable carbon source.Type: GrantFiled: February 19, 2009Date of Patent: September 10, 2013Assignee: Butalco GmbHInventors: Gunter Festel, Eckhard Boles, Christian Weber, Dawid Brat
-
Patent number: 8465945Abstract: The present invention includes a method to produce a recombinant mite Group 1 protein in a methyltrophic yeast or an Escherichia coli microorganism. The present invention also relates to a recombinant mite Group 1 protein obtained by such a method, such a recombinant protein being able to selectively bind IgE or cause proliferation of a T cell that proliferate in response to a native mite Group 1 protein. Also included in the present invention is the use of such a recombinant mite Group 1 protein to detect mite allergy or to reduce an allergic response to a mite Group 1 protein. The present invention also includes novel mite Group 1 nucleic acid molecules, proteins, recombinant molecules, and recombinant cells, as well as uses thereof.Type: GrantFiled: October 2, 2006Date of Patent: June 18, 2013Assignee: Merck Patent GmbHInventors: Elaine A. Best, Martin J. McDermott
-
Publication number: 20130109049Abstract: The present invention provides a method of infecting a nail fragment with a fungus, to form an infected nail fragment comprising at least 1×102 fungal colony forming units (cfu) per cm2 of the infected surface(s). There is also provided a method of assessing the efficacy of potential therapeutic compounds in the treatment or prevention of a fungal nail infection. The fungal nail infection may be a dermatophyte, a non-dermatophyte mould or a pathogen.Type: ApplicationFiled: December 20, 2010Publication date: May 2, 2013Applicant: NOVABIOTICS LIMITEDInventors: Deborah O'Neil, Derry Mercer, Colin Stewart
-
Patent number: 8409835Abstract: A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeast Candida sp., xylose can be effectively converted into xylitol. The invention also provides the Candida strain having high furfural tolerance, and is capable to produce xylitol from various types of non-detoxified lignocellulosic hydrolysates, in which the overall utilization of xylose in hydrolysate can reach over 95%.Type: GrantFiled: May 7, 2010Date of Patent: April 2, 2013Assignee: Institute of Nuclear Energy Research Atomic Energy Council, Executive YuanInventors: Chiung-Fang Huang, Yi-Feng Jiang, Ting-Hsiang Lin, Gia-Luen Guo, Wen-Song Hwang, Jia-Baau Wang
-
Patent number: 8389459Abstract: A composition including the fermentation supernatant from a fermentation of yeast is intended to be conveniently introduced through the wastewater plumbing system of a private home or other facility into a septic system servicing the home or other facility to substantially accelerate the ability of the bacteria resident in the septic system to substantially digest biologically available organic compounds present in the septic system, and methods of accomplishing the same.Type: GrantFiled: March 2, 2011Date of Patent: March 5, 2013Assignee: Advanced Biocatalytics CorporationInventors: John W. Baldridge, Carl W. Podella
-
Patent number: 8383373Abstract: The present invention particularly discovered strains that are capable of producing a long-chain dicarboxylic acid by culturing microorganisms belonging to Candida vini Candida entamophila, Candida blankii and Pichia farinosa which has the ability to produce a long-chain dicarboxylic acid in a liquid medium containing a straight-chain saturated hydrocarbon (tridecane) as substrate.Type: GrantFiled: December 30, 2005Date of Patent: February 26, 2013Assignee: Council of Scientific & Industrial ResearchInventors: Ahmed Kamal, Sheelu Gurrala, Wasantrao Nitin Fadnavis
-
Publication number: 20130011906Abstract: The present invention is a new additive material that is physically blended with polymeric material to create at least a partially biodegradable product.Type: ApplicationFiled: July 17, 2012Publication date: January 10, 2013Applicant: BIO-TEC ENVIRONMENTAL, LLCInventors: John Allen Lake, Samuel David Adams
-
Publication number: 20120329138Abstract: A process for separation of a mixture containing a microbial substance and a liquid using deformable filter is provided.Type: ApplicationFiled: December 19, 2011Publication date: December 27, 2012Applicant: SHELL OIL COMPANYInventors: Henry Van Kaathoven, Johannes Pieter Haan, Wilhelmus Maria Bond, Johannes Leendert Willem Cornelis Den Boestert
-
Publication number: 20120323029Abstract: Methods, biological oils, biofuels, units, and/or organisms directed to use in compression engines. A method of producing biological oils includes producing an organism and having the organism consume a feedstock. The organism includes a lipid containing fatty acids. The organism meets or exceeds at least two metrics. The metrics include: A) a cell density of at least about 115 grams per liter; B) a fatty acid content of at least about 49 percent on a dry mass basis; C) a fatty acid productivity of at least about 15 grams per liter per day; D) a fatty acid yield of at least about 0.175 grams of fatty acids produced per grams of the feedstock consumed; E) a 24 hour peak fatty acid productivity of at least about 30 grams per liter per day; F) an extraction efficiency on a percent of total fatty acid content basis of at least about 50 percent; and/or G) yield of fatty acids on oxygen of more than about 0.4 as grams of fatty acids produced per gram of oxygen consumed basis.Type: ApplicationFiled: March 8, 2011Publication date: December 20, 2012Applicant: BP Biofuels IK LimitedInventors: Joseph W. Pfeifer, III, Paul W. Behrens, Michael A. Johnson, Jon M. Hansen, Kirk E. Apt, William R. Barclay, David Christian Martin, Jacob Borden
-
Publication number: 20120295335Abstract: A method to prevent contamination of feed line(s) and nutrient feed reservoirs(s) is disclosed. In this method the growth medium which flows from the nutrient feed reservoir through the feed line to the bioreactor contains at least one nutritional component supplied at a concentration which is inhibitory to cell growth. The inhibitory nutritional component in the growth medium prevents back growth of cells from the bioreactor into the feed line(s). The growth medium with inhibitory nutritional component is diluted in the bioreactor.Type: ApplicationFiled: November 16, 2011Publication date: November 22, 2012Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventor: Scott Christopher Jackson
-
Publication number: 20120252093Abstract: Promoter regions associated with the Yarrowia lipolytica esterase/lipase (EL1) gene are disclosed and have been found to be particularly effective for the expression of heterologous genes in yeast. These promoter regions will be useful for driving high-level expression of genes involved in the production of omega-3 and omega-6 fatty acids.Type: ApplicationFiled: March 30, 2012Publication date: October 4, 2012Applicant: E I Du Pont De Nemours and CompanyInventor: Quinn Qun Zhu
-
Patent number: 8277855Abstract: The present invention provides a method of continuously producing edible lipid-based composition, including the steps: preparing alcohol and organic acid in a predetermined ratio and a reaction tank received with immobilized candida cylindracea; continuously providing a supercritical state solvent into the reaction tank and draining the supercritical state solvent out, and mixing and pressurizing the alcohol and the organic acid and sending the alcohol and the organic acid to the reaction tank for a esterfication of the alcohol and the organic acid by the candida cylindracea; getting an edible lipid-based composition and water in the reaction tank; and quickly separating the edible lipid-based composition from the water and the supercritical state solvent.Type: GrantFiled: May 7, 2010Date of Patent: October 2, 2012Inventors: Zer-Ran Yu, Be-Jen Wang
-
Publication number: 20120208230Abstract: The present invention relates to an EM-lacquer water solution mixed with a natural component, a method for preparing a fermented anti-oxidant material using the same, and a method for processing the material. Particularly, the EM-lacquer water solution mixed with a natural component is prepared by mixing an EM-lacquer solution, which is a mixture of a crude solution of effective micro-organisms (EM) 20˜35 weight percent and a lacquer water solution 65˜80 weight percent, and at least one natural component selected from a group comprising of organic brown sugar, bay salt, mandarin orange oil, Japanese apricot concentrate, Opuntia humifusa powder, dried orange peel powder, green tea powder, sea tangle powder, rice bran, camellia oil and bean powder, on the basis of the EM-lacquer water solution 20 L. Therefore, it can be used for daily life, industry, environmental purification and the like because it is made of non-toxic materials.Type: ApplicationFiled: February 13, 2012Publication date: August 16, 2012Inventor: Jeong Wan Ha
-
ACTIVE INGREDIENT THAT IS OBTAINED FROM CANDIDA SAITOANA AND COSMETIC USE FOR DETOXIFYING SKIN CELLS
Publication number: 20120164121Abstract: The object of the invention is the use of Candida saitoana or an active ingredient that is obtained from Candida saitoana as a cosmetic active ingredient, in particular for the detoxification of skin cells. The invention also relates to an active ingredient that is obtained from Candida saitoana, to a production process, as well as to cosmetic compositions that contain this active ingredient, and to a cosmetic personal care process for improving the surface condition of dull and intoxicated skin.Type: ApplicationFiled: September 3, 2010Publication date: June 28, 2012Applicant: Societe Industrielle Limousine D'Application Biologique, Dite SilabInventor: Jean Paufique -
Publication number: 20120156759Abstract: A method for the biological decomposition of organic compounds of the group of hydrocarbons, fats, oils, waxes, and derivatives thereof as well as mixtures thereof in media polluted therewith, in the presence of at least one dispersant, wherein the medium polluted with the pollutants is treated with at least one dispersant and with microorganisms.Type: ApplicationFiled: June 15, 2010Publication date: June 21, 2012Applicants: TOTAL RAFFINAGE & MARKETING, GEORG FRITZMEIER GMBH & CO. KGInventors: Ursula Fritzmeier, Robert Mallee, Achim Wiessler
-
Publication number: 20120149080Abstract: High cell density cultures of yeast were found to have higher tolerance for butanol in the medium. The high cell density yeast cultures had greater survival and higher glucose utilization than cultures with low cell densities. Production of butanol using yeast in high cell density cultures is thus beneficial for improving butanol production.Type: ApplicationFiled: June 16, 2011Publication date: June 14, 2012Applicant: Butamax(TM) Advanced Biofuels LLCInventors: VASANTHA NAGARAJAN, Michael G. Bramucci
-
Patent number: 8188028Abstract: Surfactant-containing compositions are described which include a protein component that has the effect of improving the surface-active properties of the surfactants contained in the compositions. The surfactant-containing compositions having the protein component demonstrate significantly lower critical micelle concentrations (CMC), reduced surface tensions, and reduced interfacial tensions than do comparable compositions having no protein component. In addition, the surfactant-containing compositions having the protein component has the effect of converting greasy waste contaminants to surface active materials.Type: GrantFiled: January 11, 2010Date of Patent: May 29, 2012Assignee: Advanced BioCatalytics CorporationInventors: John W. Baldridge, Carl W. Podella
-
Publication number: 20120124898Abstract: This invention provides co-cultures of photosynthetic microorganisms and biofuel producing microorganisms. In certain embodiments, polysaccharide-producing, photosynthetic microorganisms are microalgae having frustules provide a substrate on which biofuel-producing microorganisms can grow. In other embodiments, the photosynthetic microorganisms produce a lipid and the non-photosynthetic microorganisms produce a solvent in which the lipid is soluble.Type: ApplicationFiled: July 21, 2011Publication date: May 24, 2012Inventor: Pamela R. Contag
-
Patent number: 8158396Abstract: The present invention provides a novel process for ethanol fermentation which uses a high concentration of fresh yeast to co produce ethanol and good quality yeast.Type: GrantFiled: October 3, 2006Date of Patent: April 17, 2012Assignee: Alternative Fuels Corporation Pty LtdInventors: David Graham Maclennan, Mary Elizabeth Maclennan
-
Publication number: 20120090054Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: ApplicationFiled: December 19, 2011Publication date: April 12, 2012Applicants: Novozymes, Inc., Novozymes A/SInventors: Paul Harris, Elena Valsenko, Elizabeth Zaretsky, Marcus Sakari Kauppinnen
-
Publication number: 20120077181Abstract: The cryopreservation of cells and tissue cultures for long term storage of cells, cell constructs or three-dimensional complex tissues assemblies is based on the use of a collagen cell carrier (CCC) having a specific composition and thickness and appropriate mechanical properties which are maintained after thawing. The collagen cell carrier provides a suitable support for the cryopreservation resulting in high survival rates after thawing and providing cells and tissue assemblies already adhered to a mechanically stable and biocompatible support. Frozen collagen carrier-cells assemblies, the frozen artificial cell constructs or three-dimensional complex tissue assemblies obtainable by the method and the use thereof after thawing are also disclosed.Type: ApplicationFiled: February 18, 2010Publication date: March 29, 2012Inventors: Timo Schmidt, Lothar Just, Franz Maser, Holger Becker
-
Publication number: 20120034648Abstract: The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least about 70% sequence identity to the amino acid sequence set out in SEQ ID NO: 3 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.Type: ApplicationFiled: March 5, 2009Publication date: February 9, 2012Inventors: Paul Klaassen, Jan Metske Van Der Laan, Bianca Elisabeth Maria Gielesen, Gijsberdina Pieternella Van Suylekom
-
Patent number: 8097448Abstract: Cells of the species Issatchenkia orientalis and closely related yeast species are transformed with a vector to introduce an exogenous lactate dehydrogenase gene. The cells produce lactic acid efficiently and are resistant at low pH, high lactate titer conditions.Type: GrantFiled: May 30, 2006Date of Patent: January 17, 2012Assignee: Cargill Inc.Inventors: Pirkko Suominen, Aristos Aristidou, Merja Pentilla, Marja Ilmen, Laura Ruohonen, Kari Koivuranta, Kevin Roberg-Perez
-
Publication number: 20120003626Abstract: The present disclosure describes methods for concentrating microorganisms with concentration agents in a sampling device and the sampling device described herein. More specifically, methods for concentrating microorganisms from large volume samples with concentration agents in a sampling device can provide for rapid, low cost, simple (involving no complex equipment or procedures), and/or effective processes under a variety of conditions.Type: ApplicationFiled: December 30, 2009Publication date: January 5, 2012Inventors: Manjiri T. Kshirsagar, Kurt J. Halverson, Neil Percy, James E. Aysta
-
Publication number: 20120003740Abstract: This document discloses electroporation vessels, electrocompetent cells that have been aliquoted and frozen in electroporation vessels, and a number of other apparatuses, kits, and methods for electroporation. Some embodiments of electroporation vessels described herein may include a pair of opposing walls that are downwardly angled toward one another in a gap between two electrode surfaces. Further embodiments of devices and methods described herein may eliminate the need for an end user to transfer competent cells from a capped tube to electroporation cuvette, thereby saving time and producing less waste.Type: ApplicationFiled: December 28, 2010Publication date: January 5, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Laura Vozza-Brown, Harry Yim, John Cameron, Matthew O'Banion, Adam Scott Henry, Lisa Marie Olivier
-
Publication number: 20110287143Abstract: The present invention relates in general to the field of health and well-being. In particular, the present invention relates to temperature sensitive micro-organisms and their use as vehicle for the preparation of a composition to deliver compounds, for example nutrients, to specific regions of a body.Type: ApplicationFiled: July 1, 2009Publication date: November 24, 2011Applicant: NESTEC S.A.Inventors: Christof Gysler, Peter Niederberger, Nicolas Page, Christelle Schaffer-Lequart
-
Publication number: 20110223288Abstract: The present invention relates to a method for producing an alanine-rich yeast containing alanine at a high concentration and includes: a method involving liquid-culturing a yeast in a stationary phase of proliferation under the condition that the pH of a liquid medium is ?7.5 and <11; a method for producing an alanine-rich yeast by adjusting the pH of a liquid medium to ?7.5 and <11, and then culturing the yeast in this pH range; a method of producing an alanine-rich yeast, wherein the yeast is Saccharomyces cerevisiae or Candida utilis; an alanine-rich yeast obtained by any one of said methods; an alanine-rich yeast extract extracted from said alanine-rich yeast; a seasoning composition including said alanine-rich yeast extract; and an alanine-containing food and drink including said alanine-rich yeast, said alanine-rich yeast extract or said seasoning composition.Type: ApplicationFiled: November 17, 2009Publication date: September 15, 2011Applicant: ASAHI BREWERIES, LTD.Inventors: Ichiro Shibuya, Tetsuji Odani
-
Publication number: 20110223287Abstract: Provided is a method for producing a yeast containing glutamic acid at a high concentration. In the method for culturing a yeast, a yeast in a stationary growth phase is subjected to liquid culture under the conditions that the pH of a liquid medium is 7.5 or higher and lower than 11. After the pH of the liquid medium for the yeast in a stationary growth phase is adjusted to 7.5 or higher and lower than 11, the yeast is further cultured, whereby a yeast with a high glutamic acid content can be produced. In the invention, as the yeast, Saccharomyces cerevisiae or Candida utilis can be used. Therefore, by using the yeast with a high glutamic acid content obtained by the above-said method and the extract obtained by extraction from the yeast, a seasoning composition and a food or drink with a high glutamic acid content can be provided.Type: ApplicationFiled: October 30, 2009Publication date: September 15, 2011Applicant: Asahi Breweries, Ltd.Inventors: Ichiro Shibuya, Hiroaki Okano, Yoshitomo Kanaoka, Nobuchika Takesue
-
Publication number: 20110206823Abstract: The present invention provides: a method for producing an amino acid-rich yeast containing free amino acid in high concentration; a method for producing an amino acid-rich yeast, which includes liquid-culturing a yeast in a stationary phase of proliferation wherein the pH of a liquid medium is ?7.5 and <11; a method for producing an amino acid-rich yeast, which includes adjusting the pH of the liquid medium to ?7.5 and <11, and further culturing the yeast in this pH range; a method for producing an amino acid-rich yeast, wherein the yeast is Saccharomyces cerevisiae or Candida utilis; an amino acid-rich yeast obtained by any one of the above methods; the amino acid-rich yeast extract wherein the amount of a free amino acid is from 7.5 to 18.0% by weight per dry yeast cell; and an amino acid-rich yeast extract extracted from the above amino acid-rich yeast.Type: ApplicationFiled: November 17, 2009Publication date: August 25, 2011Inventors: Ichiro Shibuya, Tetsuji Odani
-
Publication number: 20110201093Abstract: The present invention is directed to the enhanced production of ethanol from fermentation using an ethanol producing microorganism. In particular, the present invention provides a process for the enhanced growth and metabolism of an ethanol producing microorganism (e.g., yeast), for the purpose of increasing or enhancing ethanol production, both in terms of total ethanol produced and in terms of the time required for ethanol producing microorganisms to convert carbohydrates and/or sugars to end products (i.e., ethanol). In accordance with this invention bicarbonate ions are used to enhance ethanol fermentation and/or reduce the time required for ethanol producing microorganisms to convert carbohydrates and/or sugars to end products, i.e., ethanol. The present invention can also be used for enhancing fermentation end products in, for example, the fuel ethanol, industrial, cheese, brewing, and wine making industries.Type: ApplicationFiled: April 28, 2011Publication date: August 18, 2011Applicant: CHURCH & DWIGHT CO., INC.Inventors: Melvin H. Czechowski, Steven A. Bolkan