Culture Media, Per Se, Or Technique Patents (Class 435/255.7)
  • Patent number: 6846657
    Abstract: The invention relates to a process for the simultaneous production of xylitol and ethanol from a hydrolyzed lignocellulose-containing material starting. The starting material is fermented with a yeast strain, the ethanol produced is recovered, a chromatographic separation is carried out on the remaining xylitol solution, and pure xylitol is crystallized.
    Type: Grant
    Filed: September 12, 1997
    Date of Patent: January 25, 2005
    Assignee: Xyrofin Oy
    Inventors: Heikki Heikkilä, Göran Hyöky, Leena Rahkila, Marja Leena Sarkki, Tapio Viljava
  • Patent number: 6638755
    Abstract: A simple culture medium produced by impregnating a fibrous water-absorbent sheet with a suspension formed by suspending in an alcohol (a) an adhesive (0.01-0.4 wt. %) which is soluble in both water and alcohol, (b) a gelling agent which is soluble in water and insoluble in alcohol, and (c) a bacterial nutritive ingredient, the fibrous water-absorbent sheet having a mesh larger than the particle size of the gelling agent and being placed on a waterproof flat plate, and by drying the resultant sheet while suppressing rapid evaporation of the alcohol, to thereby cause the water-absorbent sheet to adhere onto the waterproof flat plate; and a method for producing the medium.
    Type: Grant
    Filed: November 19, 2001
    Date of Patent: October 28, 2003
    Assignee: Nissui Pharmaceutical Co., Ltd.
    Inventors: Shingo Mizuochi, Hidemasa Kodaka, Hideaki Shibata
  • Patent number: 6548288
    Abstract: The present invention discloses high density antagonistic microbe base materials and a method of producing the same, more particularly, a method for preparing high density antagonistic microbe base material comprising the steps of: inoculating a mixture of native microbes as main components to organic medium to obtain crude bacteria by subculture; and further inoculating the obtained crude bacteria in a culture medium consisting of crushed soil from volcanic rocks mineral, combined nutrients and water, culturing and then being subjected to air ventilation under agitating the cultured material along the rise in temperature.
    Type: Grant
    Filed: October 2, 2001
    Date of Patent: April 15, 2003
    Inventor: Shinya Tsuru
  • Patent number: 6468779
    Abstract: An aqueous extract of legumes is prepared for growing agronomically beneficial microorganisms. The extract is prepared by boiling a legume in water and removing solids. Preferably, 150 to 250 grams of legumes are boiled in about one liter of water for 15 to 30 minutes. The legumes include peas, beans, clover, alfalfa, and mixtures thereof. Also pea seeds can be prepared as a legume aqueous extract. A nitrogen source, a salt of an organic acid, an organic alcohol or mixtures thereof can be added to the extract-containing medium. Furthermore, the extract-containing medium is effective for growing agronomically beneficial microbes to a cell density of 108 cells per milliter.
    Type: Grant
    Filed: August 9, 1999
    Date of Patent: October 22, 2002
    Assignee: Tatko Biotech, Inc.
    Inventor: Tatiana Vasilienva Red'kina
  • Patent number: 6461857
    Abstract: Provided is a process of and product resulting from adding a sub-lethal amount of a peroxide to a growing culture of yeast, observing the change in absorbance of the culture at 256 nm as a measure of the injury occurring to the yeast cells, and, upon reaching a predetermined absorbance value, purifying the water-soluble yeast extract from the peroxide containing yeast culture. The resulting product has at least 1.5 respiratory units per mg dry weight and has significantly increased amounts of 15, 25 and 55 kD molecular weight products compared to a Live Yeast Cell Derivative as determined by SDS slab gel electrophoresis. This product can be combined with a cosmetically acceptable carrier to provide a composition suitable for topical application to the skin.
    Type: Grant
    Filed: July 19, 2000
    Date of Patent: October 8, 2002
    Assignee: Arch Personal Care Products, L.P.
    Inventors: Durant B. Scholz, Derek Parish, Hans Schaeffer
  • Publication number: 20020058299
    Abstract: A new, novel and useful mycologic genera specific selective agar method of manufacture and use. One of the benefits that is derived from this agar is the ability to quickly and easily selectively grow user selected mycologic genera which include the development of characteristic colony morphology, coloration and spore arrangement and texture. The growth medium or agar contains user specified anti-fungal and anti-bacterial compounds that inhibit the growth and development of competitive fungi and bacteria.
    Type: Application
    Filed: November 13, 2001
    Publication date: May 16, 2002
    Inventors: Richard A. Billups, Paul S. Warden, Kristen S. Fallon, Jennifer E. Parent
  • Patent number: 6290950
    Abstract: The present invention pertains to vaccines comprising homogenised inactivated yeast blastospores and homogenised inactivated dermatophyte microconidia or antigenic material of said spores, methods for their production and their use for the prophylaxis and/or treatment of mycoses in mammals, preferably humans. The vaccines according to the present invention are especially useful for the prophylaxis and/or treatment of skin mycosis, preferably Dermatomycosis and/or Candidosis and/or Onychomycosis.
    Type: Grant
    Filed: May 5, 1999
    Date of Patent: September 18, 2001
    Inventors: Igor Dimitrievich Poliakov, Ludmilla Ivanova
  • Patent number: 6265203
    Abstract: A culture material is formed containing a water-soluble polymer layer and a porous matrix layer arranged such that when water is applied to the surface of the matrix layer the water diffuses through the matrix layer to dissolve the water-soluble polymer layer which then gradually penetrates into the matrix layer to form a single layer for use as a culture medium on which a microorganism can be grown to form colonies. The water-soluble polymer layer or water added to the matrix layer can contain nutrients for the microorganism, and the microorganism can be added with the water. The culture material has a water content of not more than 50%, and is sufficiently insoluble in water such that it substantially holds its original shape for at least 30 minutes when in contact with water having a temperature of not more than 50° C.
    Type: Grant
    Filed: June 29, 1998
    Date of Patent: July 24, 2001
    Assignee: Chisso Corporation
    Inventor: Masashi Ushiyama
  • Patent number: 6172006
    Abstract: The invention relates to biologically active novel compounds having formula (I) as defined herein. Also disclosed are methods of preparing said compounds, fungicidal compositions comprising, as an active ingredient, these compounds, use of the compounds, and method of controlling fungi at loci infested or liable to be infested therewith.
    Type: Grant
    Filed: March 18, 1998
    Date of Patent: January 9, 2001
    Assignee: Novo Nordiskals
    Inventors: Helle Demuth, Jens Breinholt, Birgitte Rassing Romer