Abstract: Despite efforts of eradication and sanitation, Phytophthora ramorum persists in the United States and has been a problem in commercial nurseries since 2001. Detection in a nursery immediately causes the nursery to be under quarantine until the P. ramorum can be proven to be eradicated. Treatment with the biological control agent Trichoderma asperellum showed promise in laboratory results to remediate P. ramorum-infested potting soil. Sixteen T. asperellum isolates were screened, found to have variable mycoparasitic activities, and further tested in natural field settings. Field plots having P. ramorum-infested soil were treated with T. asperellum, a chemical and two different commercial biological control products and compared to non-treated infested soil. After 8 weeks, P. ramorum populations were not detected in the soil treated with T. asperellum isolate 04-22 showing that T. asperellum isolate 04-22 could remediate soils infested with P. ramorum under natural field conditions.

Abstract: The present invention relates to a method of producing cellulolytic and/or hemicellulolytic enzymes by a cellulolytic microorganism in a stirred and aerated bioreactor, comprising a growth stage in the presence of a carbon source and a production stage in the presence of a carbon-containing inductive substrate, wherein the supply of carbon-containing inductive substrate during the production stage is regulated by an oscillation of the dissolved oxygen partial pressure in the medium.

Abstract: Described herein are compositions and methods related to isolated Trichoderma harzianum and strains thereof.

Abstract: The invention relates to fungal strains having at least one genetic modification which leads to a reduction of the activity of at least one fungal carboxylic acid transporter and to a method for producing or using said fungal strains.

Abstract: A method of chemically treating partially de-ashed pulp and/or paper mill sludge to obtain products of value comprising taking a sample of primary sludge from a Kraft paper mill process, partially de-ashing the primary sludge by physical means, and further treating the primary sludge to obtain the products of value, including further treating the resulting sludge and using the resulting sludge as a substrate to produce cellulase in an efficient manner using the resulting sludge as the only carbon source and mixtures of inorganic salts as the primary nitrogen source, and including further treating the resulting sludge and using the resulting sludge to produce ethanol.

Abstract: The present invention relates to a fertilizer comprising at least one microbe selected from the group consisting of Trichoderma viride (NRRL B-50520), Scopulariopsis brevicaulis (NRRL B-50521), Scopulariopsis brevicaulis (NRRL B-50522), and combinations thereof, and a substrate which is acted upon by the at least one microbe to release nitrogen. The invention also relates to a fertilizer comprising at least one microbe selected from a keratin degrading microorganism and a keratin substrate which is acted upon by the at least one microbe to release nitrogen. The invention further relates to a method for enhancing growth of plants with the fertilizer, and methods of making a fertilizer. The invention further relates to an isolated Trichoderma viride strain (NRRL B-50520) and two isolated Scopulariopsis brevicaulis strains (NRRL B-50521, NRRL B-50522).

Abstract: The invention relates to a method for multiplying a strain of Trichoderma, which includes the preparation of substantially contaminant-free culture media, containing disinfected wood fibers obtained by means of a twin-screw extruder, the amplification of Trichoderma via a series of steps for manufacturing a primary inoculum and then a secondary inoculum from said primary inoculum, and the multiplication of the microorganisms in each step, wherein the multiplication of the propagules reaches 2×104 to 105. The invention also relates to a plant growing medium containing a secondary inoculum of Trichoderma.

Abstract: The present invention relates to glucoamylase variants having improved thermostability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.

Abstract: A method is described for producing protein compositions having reduced amounts of O-linked glycosylation. The method includes producing the protein in cells cultured in the presence of an inhibitor of Pmt-mediated O-linked glycosylation and/or in the presence of one or more ?-1,2-mannosidases.

Abstract: The present invention relates to a method for enhancing growth of plants which comprises contacting a Trichoderma strain with the plant or a plant seed under conditions effective for the Trichoderma strain to colonize the roots of the plant or a plant grown from the plant seed, thereby creating a plant-Trichoderma system. The plant or plant seed is grown under conditions effective to sustain the plant-Trichoderma system in a planting medium and to enhance plant growth, where the Trichoderma strain is selected from the group consisting of Trichoderma atroviride strain WW10TC4 (ATCC accession number PTA 9707), Trichoderma harzianum strain RR17Bc (ATCC accession number PTA 9708), Trichoderma harzianum strain F11Bab (ATCC accession number PTA 9709), and combinations thereof.

Abstract: The present invention relates to a method for enhancing growth of plants which comprises contacting a Trichoderma strain with the plant or a plant seed under conditions effective for the Trichoderma strain to colonize the roots of the plant or a plant grown from the plant seed, thereby creating a plant-Trichoderma system. The plant or plant seed is grown under conditions effective to sustain the plant-Trichoderma system in a planting medium and to enhance plant growth, where the Trichoderma strain is selected from the group consisting of Trichoderma atroviride strain WW10TC4 (ATCC accession number PTA 9707), Trichoderma harzianum strain RR17Bc (ATCC accession number PTA 9708), Trichoderma harzianum strain F11Bab (ATCC accession number PTA 9709), and combinations thereof.

Abstract: Some aspects of this invention relate to methods useful for the conversion of a carbon source to a biofuel or biofuel precursor using engineered microbes. Some aspects of this invention relate to the discovery of a key regulator of lipid metabolism in microbes. Some aspects of this invention relate to engineered microbes for biofuel or biofuel precursor production.

Abstract: The invention provides for methods for producing isoprene from cultured cells using various components of the DXP pathway and MVA pathway, or components associated with the DXP pathway and MVA pathway, iron-sulfur cluster-interacting redox polypeptides, and isoprene synthase. The invention also provides compositions that include these cultured cells.

Abstract: The present invention relates to a host cell deficient in an essential gene, comprising a vector, said vector comprising at least said essential gene and an autonomous replication sequence, wherein the host cell is a filamentous fungal cell. The invention also relates to a host cell deficient in an essential gene, comprising a vector, said vector comprising at least said essential gene and an autonomous replication sequence, wherein the host cell comprises a recombinant polynucleotide construct comprising a polynucleotide encoding a biological compound of interest or a compound involved in the synthesis of a biological compound of interest.

Abstract: The present invention relates to methods for the screening, identification and/or application of microorganisms of use in imparting beneficial properties to plants.

Abstract: A novel strain of Trichoderma harzianum called TSTh20-1 is described. TSTh20-1 is useful in promoting plant growth, increasing water use efficiency of plants and in remediation of soil or water.

Abstract: The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 73% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 73% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

Abstract: A method is described for producing protein compositions having reduced amounts of O-linked glycosylation. The method includes producing the protein in cells cultured in the presence of an inhibitor of Pmt-mediated O-linked glycosylation and/or in the presence of one or more ?-1,2-mannosidases.

Abstract: The present invention provides a method of infecting a nail fragment with a fungus, to form an infected nail fragment comprising at least 1×102 fungal colony forming units (cfu) per cm2 of the infected surface(s). There is also provided a method of assessing the efficacy of potential therapeutic compounds in the treatment or prevention of a fungal nail infection. The fungal nail infection may be a dermatophyte, a non-dermatophyte mould or a pathogen.

Abstract: An embodiment of the present invention is a Trichoderma atroviride SC1, CBS no 122089, as a biocontrol agent, i.e. to treat fungal diseases of plants. The second embodiment of the present invention is an agricultural composition comprising the Trichoderma atroviride SC1 as the active principle in an effective amount. The compositions of the present invention may further comprise a second biocontrol agent and/or an additive, an emulsifier, a plant nutrient, a wetting-agent, a plant micro-nutrient or a substratum, wherein said substratum is selected from the group consisting of: a nutrient culture medium, a cereal or a derivative thereof, an amendant, a vegetable or a part thereof, peat, wood or a piece thereof, clay or barks.

Abstract: The present invention relates to Trichoderma atroviride strains and their use as biological control agents or plant growth promoters. Methods and compositions for biological control of soil borne plant pathogens, and increasing plant yield using T. atroviride are also provided.

Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.

Abstract: The present invention is a new additive material that is physically blended with polymeric material to create at least a partially biodegradable product.

Abstract: The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.

Abstract: A process for separation of a mixture containing a microbial substance and a liquid using deformable filter is provided.

Abstract: A process for obtaining a treated backset for use in ethanol production, the process involving treating waste material from an ethanol fermentation process with microorganisms to remove or decrease microbial cell growth inhibitors present in the waste material; and removing microbial biomass from the treated waste material to form a treated backset. The treated backset can be used in a process for producing ethanol from a fermentable biomass. Recovered microbial biomass can be used in as an animal feed or as a dietary supplement.

Abstract: To produce cellulase having excellent ability to decompose cellulosic resources containing xylan at low cost. A method for producing ?-glucanase and xylanase, comprising the step of culturing a microorganism classified under the genus Trichoderma by using a liquid culture medium which contains (a) wheat bran as a carbon source and (b) ammonia nitrogen or amino nitrogen as a nitrogen source.

Abstract: Disclosed is production of cellulase having excellent ability to decompose cellulosic resources containing xylan at low cost. A method for producing ?-glucanase and xylanase, comprising the step of culturing a microorganism classified under the genus Trichoderma by using a liquid culture medium which contains fungus body debris as an organic carbon source.

Abstract: A novel strain of Trichoderma harzianum called TSTh20-1 is described. TSTh20-1 is useful in promoting plant growth, increasing water use efficiency of plants and in remediation of soil or water.

Abstract: A method is described for producing protein compositions having reduced amounts of O-linked glycosylation. The method includes producing the protein in cells cultured in the presence of an inhibitor of Pmt-mediated O-linked glycosylation and/or in the presence of one or more ?-1,2-mannosidases.

Abstract: This invention provides co-cultures of photosynthetic microorganisms and biofuel producing microorganisms. In certain embodiments, polysaccharide-producing, photosynthetic microorganisms are microalgae having frustules provide a substrate on which biofuel-producing microorganisms can grow. In other embodiments, the photosynthetic microorganisms produce a lipid and the non-photosynthetic microorganisms produce a solvent in which the lipid is soluble.

Abstract: The present invention relates to methods for monitoring differential expression of a plurality of genes in a first filamentous fungal cell relative to expression of the same genes in one or more second filamentous fungal cells using microarrays containing Trichoderma reesei ESTs or SSH clones, or a combination thereof. The present invention also relates to computer readable media and substrates containing such array features for monitoring expression of a plurality of genes in filamentous fungal cells.

Abstract: A method for enhancing growth of plants comprising contacting a Trichoderma strain with the plant or a plant seed under conditions effective for the Trichoderma strain to colonize the roots of the plant or a plant grown from the plant seed, thereby creating a plant-Trichoderma system. The plant or plant seed is grown under conditions effective to sustain the plant-Trichoderma system in a planting medium and to enhance plant growth. The Trichoderma strain is selected from the group consisting of Trichoderma atroviride strain WW10TC4 (ATCC accession number PTA 9707), Trichoderma harzianum strain RR17Bc (ATCC accession number PTA 9708), Trichoderma harzianum strain F11 Bab (ATCC accession number PTA 9709), and combinations thereof. Methods of enhancing resistance of plants to abiotic stress, increasing nitrogen use efficacy in plants, reducing nitrous oxide emissions in air, reducing leaching of nitrates into soil and water, and enhancing sequestration of carbon from air are disclosed.

Abstract: A seed treated with a fungal/bacterial antagonist combination and a seed assembly comprising a seed and a fungal/bacterial antagonist combination. The fungal/bacterial antagonist combination comprises a Trichoderma virens fungal antagonist and a Bacillus amyloliquefaciens bacterial antagonist for controlling plant pathogens as a biocontrol agent, bio-pesticide or bio-fungicide. In preferred embodiments, the invention produces an increase in plant yield. Control of early and late season stalk and root rot caused by fungi such as Fusarium, Phythium, Phytophthora and Penicillium in tomatoes, peppers, turf grass, soybeans, sunflower, wheat and corn is achieved.

Abstract: Described are compositions and methods relating to variant alpha-amylases having altered biochemical properties and advantageous performance characteristics as compared to a reference alpha-amylase. The variants are suitable for use in various industrial applications such as starch conversion, ethanol production, laundry, dishwashing, pulp and paper production, textile desizing, and/or sweetener production.

Abstract: The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least about 70% sequence identity to the amino acid sequence set out in SEQ ID NO: 3 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.

Abstract: The invention provides a method for the expression and subsequent screening of DNA libraries, particularly synthetic, genomic, and cDNA libraries, in filamentous fungal hosts. In particular, the invention provides vectors, host strains, and a method for the expression and screening of complex DNA libraries, including, but not limited to, combinatory (combinatorial) libraries expressing one, two or more variable constituents and/or prepared from two or more sublibraries (e.g., for the expression and screening of immunoglobulin (including fragments and derivatives of whole immunoglobulin proteins) and other receptor or complex DNA libraries or libraries of libraries). The invention is useful for the expression and screening for a large variety of proteins and protein complexes, including human proteins. The present invention also relates to novel fungal protease sequences.

Abstract: An inoculant composition comprising fungal spores applied to a carrier having a moisture content of not more than about 5% is provided. A method of inoculating a plant to promote growth, enhance resistance to adverse conditions or promote re-growth is also provided comprising applying the inoculant composition to the plant.

Abstract: The present invention relates to a biocontrol strain against the wilt of continuous cropping cucumber and watermelon as well as its microbial organic fertilizer, which belongs to the technology for agricultural intensive production. The present invention separates Trichoderma harzianum SQR-T037, which has a remarkable biocontrol effect against wilt of cucumber and watermelon. Microbial organic fertilizer was produced from this biocontrol strain and organic compost. In this fertilizer, the content of the strain is above 1×108 cfu/g, the content of total nitrogen is 3˜5% (above 90% of the nitrogen is organic nitrogen), total nitrogen-phosphorus-kalium nutrient is 6˜10% and organic matter is 30˜35%. As indicated by the experiments, after this microbial organic fertilizer is applied to soil, it will enable rapid multiplication of biocontrol strain into a dominant microflora in the soil and achieve more than 85% of biocontrol rate of wilt in the soil suffering from serious cucumber and watermelon wilt.

Abstract: N-Acetyl-D-glucosamine can be produced by cultivating a fungus capable of producing N-acetyl-D-glucosamine, such as Trichoderma hamatum AB10282 strain (FERM BP-10623) or Trichoderma harzianum AB10283 strain (FERM BP-10624), in a culture medium supplemented with a carbon source other than chitin and chitin oligosaccharide and a nitrogen source to produce and accumulate N-acetyl-D-glucosamine in the culture medium and then collecting N-acetyl-D-glucosamine from the culture medium.

Abstract: The present invention provides methods for screening for a desired filamentous fungus that produces cellulase more efficiently and/or with increased specific activity. It also provides filamentous fungi that produce cellulase more efficiently.

Abstract: The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.

Abstract: Provided is a method of modifying fungal morphology. The method comprises introducing an amount of a surfactant to a fungus, or to a growing mixture comprising a fungus, wherein said amount of a surfactant is sufficient to induce a change in the morphology of said fungus.

Abstract: The present invention includes wickerol and a method for producing the wickerol. In the method for producing the wickerol, Trichoderma atroviride strain FKI-3737 (FERM ABP-11099 corresponding to FERM BP-11099) belonging to filamentous fungi is cultured in a medium, and the wickerol is accumulated in a culture, and then the produced wickerol is isolated and purified from the culture. A substance having inhibitory activity against influenza virus replication and containing the wickerol as an active ingredient, and an anti-influenza drug containing the wickerol as an active ingredient are obtained.

Abstract: An embodiment of the present invention is a Trichoderma atroviride SC1, CBS n° 122089, as a biocontrol agent, i.e. to treat fungal diseases of plants. The second embodiment of the present invention is an agricultural composition comprising the Trichoderma atroviride SC1 as the active principle in an effective amount. The compositions of the present invention may further comprise a second biocontrol agent and/or an additive, an emulsifier, a plant nutrient, a wetting-agent, a plant micro-nutrient or a substratum, wherein said substratum is selected from the group consisting of: a nutrient culture medium, a cereal or a derivative thereof, an amendant, a vegetable or a part thereof, peat, wood or a piece thereof, clay or barks.

Abstract: The present invention relates to Trichoderma atroviride strains and their use as biological control agents or plant growth promoters. Methods and compositions for biological control of soil borne plant pathogens, and increasing plant yield using T. atroviride are also provided.

Abstract: The present invention relates to wastewater treatment in general and to methods of controlling odors and degrading compounds contained in wastewater in particular.

Abstract: Methods, enzymes, recombinant microorganism, and microbial systems are provided for converting suitable monosaccharides or oligosaccharides, such as those derived from biomass, as well as various aldehydes and/or ketones, into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided. Commodity chemical enriched, refinery-produced petroleum products are also provided, as well as methods for producing the same.

Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.