Transformants Patents (Class 435/257.2)
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Patent number: 8969067Abstract: There is provided a process of growing a phototrophic biomass in a reaction zone. Gaseous exhaust material is produced with a gaseous exhaust material producing process, wherein the gaseous exhaust material includes carbon dioxide. A discharge of the gaseous exhaust material is supplied to the reaction zone and is modulated. A bypass gaseous exhaust material, being supplied to another unit operation, is also modulated.Type: GrantFiled: May 20, 2010Date of Patent: March 3, 2015Assignee: Pond Biofuels Inc.Inventors: Steven C. Martin, Max Kolesnik, Jaime A. Gonzalez
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Patent number: 8969066Abstract: The present invention provides compositions and methods for producing flocculation moieties in photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of the flocculation moieties. Also provided are methods of flocculating organisms.Type: GrantFiled: June 26, 2009Date of Patent: March 3, 2015Assignee: Sapphire Energy, Inc.Inventors: Michael Mendez, Craig Behnke, Yan Poon, Philip Lee
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Publication number: 20150056707Abstract: Provided herein are compositions and methods for engineering salt tolerance and producing products by photosynthetic organisms. The photosynthetic organisms can be genetically modified to be salt tolerant as compared to an unmodified organism and to produce useful products. The methods and compositions of the disclosure are useful in many therapeutic and industrial applications.Type: ApplicationFiled: November 4, 2014Publication date: February 26, 2015Applicant: SAPPHIRE ENERGY, INC.Inventors: MICHAEL MENDEZ, SU-CHIUNG FANG, STEPHANE RICHARD
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Patent number: 8962299Abstract: The invention relates to methods for producing a wax ester in recombinant host cells engineered to express a thioesterase, an acyl-CoA synthetase, an alcohol-forming fatty acyl reductase, and a wax ester synthase. The methods of the invention may take place in photosynthetic microorganisms, and particularly in cyanobacteria. Isolated nucleotide molecules and vectors expressing the thioesterase, acyl-CoA synthetase, alcohol-forming fatty acyl reductase, and wax ester synthase, recombinant host cells expressing the thioesterase, acyl-CoA synthetase, alcohol-forming fatty acyl reductase, and wax ester synthase, and systems for producing a wax ester via a pathway using these four enzymes, are also provided.Type: GrantFiled: February 29, 2012Date of Patent: February 24, 2015Assignee: ExxonMobil Research and Engineering CompanyInventors: Erik Holtzapple, John H. Verruto
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Antigenic mimics of discontinuous epitopes of pathogen recognized by broadly neutralizing antibodies
Patent number: 8961977Abstract: The present invention relates to an anti-idiotypic polypeptide scaffold that includes two or more peptide sequences that mimic a discontinuous epitope of a pathogen that is recognized by or induces formation of a broadly neutralizing antibody. Using a fibronectin FNfn10 scaffold bearing two or more modified discontinuous loops, scaffolds that recognize broadly neutralizing antibodies in vitro and from patient serum have been identified. These scaffolds should induce an immune response or mobilize germline specificities to initiate their affinity maturation.Type: GrantFiled: February 14, 2011Date of Patent: February 24, 2015Assignee: University of RochesterInventors: Stephen Dewhurst, Mark A. Sullivan -
Patent number: 8962300Abstract: The present invention provides reagents and methods for biomaterial production from cyanobacteria.Type: GrantFiled: June 6, 2013Date of Patent: February 24, 2015Assignee: Arizona Board of Regents, a Body Corporate Acting for and on Behalf of Arizona State UniversityInventors: Hatem Mohammed, Wim Vermaas
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Publication number: 20150050253Abstract: It can be useful to regulate the growth of microbial cells. Some embodiments herein provide genetically engineered microbial cells that can produce bacteriocins to control the growth of microbial cells. In some embodiments, microbial cells are contained within a desired environment. In some embodiments, contaminating microbial cells are neutralized. In some embodiments, a first microbial cell type regulates the growth of a second microbial cell type so as to maintain a desired ratio of the two cell types.Type: ApplicationFiled: August 14, 2014Publication date: February 19, 2015Inventor: Philippe Gabant
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Publication number: 20150050739Abstract: The present invention relates to a method for carrying out recombination at a target locus.Type: ApplicationFiled: March 12, 2013Publication date: February 19, 2015Inventors: Noel Nicolaas Maria Elisabeth Van Peij, Martina Beishuizen, Yvonne Johannes Odilia Arendsen
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Publication number: 20150044749Abstract: Compositions and methods for producing aldehydes, alkanes, and alkenes are described herein. The aldehydes, alkanes, and alkenes can be used in biofuels.Type: ApplicationFiled: August 28, 2014Publication date: February 12, 2015Applicant: REG LIFE SCIENCES, LLCInventors: Andreas W. SCHIRMER, Mathew A. RUDE, Shane A. BRUBAKER
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Publication number: 20150044747Abstract: The invention relates, in part, to nucleic acid constructs, genetically modified host cells and methods employing such constructs and host cells to increase the production of 3-methyl-2-butenol from IPP. Thus, in some aspects, the invention provides a genetically modified host cell transformed with a nucleic acid construct encoding a fusion protein comprising a phosphatase capable of catalyzing the dephosphorylation of dimethylallyl diphosphate (DMAPP) linked to an IPP isomerase capable of converting IPP to DMAPP, wherein the nucleic acid construct is operably linked to a promoter. In some embodiments, the genetically modified host cell 5 further comprises a nucleic acid encoding a reductase that is capable of converting 3-methyl-2-butenol to 3-methyl-butanol. In some embodiments, the reductase is encoded by a nucleic acid construct introduced into the cell. In some embodiments, the IPP isomerase is a Type I isomerase. In some embodiments, the IPP isomerase is a Type II isomerase.Type: ApplicationFiled: September 13, 2012Publication date: February 12, 2015Applicant: The Regents of the University of CaliforniaInventors: Howard Chou, Jay D. Keasling
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Patent number: 8951762Abstract: A method of increasing production of fatty acids comprising introducing into a host cell or organism and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from Bryantella formatexigens or a mutant thereof; a method of making a mutant B. formatexigens acyl-ACP TE; a method of making a chimeric Cuphea viscosissima acyl-ACP TE; a nucleic acid encoding a mutant acyl-ACP TE or a chimeric C. viscosissima acyl-ACP TE; a host cell or organism comprising the nucleic acid; a mutant acyl-ACP TE or chimeric C. viscosissima acyl-ACP TE; a method of altering the specificity of a plant acyl-ACP TE; and a method of altering the level of activity of a plant acyl-ACP TE.Type: GrantFiled: July 25, 2012Date of Patent: February 10, 2015Assignee: Iowa State University Research Foundation, Inc.Inventors: Basil J. Nikolau, Marna Yandeau-Nelson, Fuyuan Jing
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Publication number: 20150037370Abstract: This invention provides diatom-based vaccines.Type: ApplicationFiled: October 26, 2012Publication date: February 5, 2015Applicants: SYNAPTIC RESEARCH, LLC, THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Lynette B. Corbeil, Mark Hildebrand, Roshan Shrestha, Aubrey Davis, Rachel Schrier, George A. Oyler, Julian N. Rosenberg
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Publication number: 20150037892Abstract: The present invention relates to a method for carrying out recombination at a target locus.Type: ApplicationFiled: March 12, 2013Publication date: February 5, 2015Inventors: Nathalie Wiessenhaan, Catharina Petronella Antonia Maria Kolen, Bernard Meijrink, Viktor Marius Boer, Johannes Andries Roubos, Yvonne Johannes Odilia Arendsen
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Publication number: 20150037301Abstract: The invention relates to a recombinant factor VIII that includes one or more mutations at an interface of A1 and C2 domains of recombinant factor VIII. The one or more mutations include substitution of one or more amino acid residues with either a cysteine or an amino acid residue having a higher hydrophobicity. This results in enhanced stability of factor VIII. Methods for making the recombinant factor VIII, pharmaceutical compositions containing the recombinant factor VIII, and use of the recombinant factor VIII for treating hemophilia A are also disclosed.Type: ApplicationFiled: October 19, 2014Publication date: February 5, 2015Applicant: UNIVERSITY OF ROCHESTERInventors: Philip J. Fay, Hironao Wakabayashi
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Publication number: 20150037849Abstract: Recombinant microbial cells and methods for producing 5-hydroxytryptophan (5HTP) using such cells are described. More specifically, the recombinant microbial cell comprises an exogenous gene encoding an L-tryptophan hydroxylase, and means for providing tetrahydrobiopterin (THB). Related sequences and vectors for use in preparing such recombinant microbial cells are also described.Type: ApplicationFiled: February 28, 2013Publication date: February 5, 2015Inventors: Eric Michael Knight, Jiangfeng Zhu, Jochen Förster, Hao Luo
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Patent number: 8945926Abstract: This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.Type: GrantFiled: December 16, 2011Date of Patent: February 3, 2015Assignee: The Scripps Research InstituteInventors: Jason W. Chin, T. Ashton Cropp, J. Christopher Anderson, Peter G. Schultz
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Patent number: 8945908Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.Type: GrantFiled: April 18, 2013Date of Patent: February 3, 2015Assignee: Solazyme, Inc.Inventors: Scott Franklin, Aravind Somanchi, George Rudenko, Riyaz Bhat, Xinhua Zhao, Risha Bond, Walter Rakitsky, Alejandro Marangoni, Diza Braksmayer
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Patent number: 8940884Abstract: The present invention is directed to isolated nucleic acid molecules and polypeptides of thraustochytrid polyunsaturated fatty acid (PUFA) synthases involved in the production of PUFAs, including PUFAs enriched in docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), or a combination thereof. The present invention is directed to vectors and host cells comprising the nucleic acid molecules, polypeptides encoded by the nucleic acid molecules, compositions comprising the nucleic acid molecules or polypeptides, and methods of making and uses thereof.Type: GrantFiled: March 19, 2010Date of Patent: January 27, 2015Assignee: DSM IP Assets B.V.Inventors: Kirk E. Apt, Leslie Richter, David Simpson, Ross Zirkle
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Publication number: 20150026840Abstract: To produce a bacterial microcompartment shell, or a designed shell based on naturally occurring bacterial microcompartment shells in a new host organism, a synthetic operon is constructed that contains the desired shell protein genes and translation efficiency is controlled by host specific ribosomal binding sites. Proteins or other molecules can be encapsulated in the microcompartment shells by various methods described herein. The constructs can also be used to express self-assembling sheets comprised of shell proteins.Type: ApplicationFiled: March 14, 2014Publication date: January 22, 2015Applicant: The Regents of the University of CaliforniaInventors: Cheryl A. Kerfeld, Jonathan K. Lassila, James N. Kinney
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Publication number: 20150024442Abstract: Provided are fusion proteins of diacylglycerol acyltransferase (DGAT) polypeptides fused to at least one heterologous intracellular localization domain, such as a bacterial membrane-targeting domain, modified photosynthetic microorganism that comprise such fusion proteins, and related methods for improving the production of lipids by photosynthetic microorganisms, including lipids such as triglycerides, fatty acids, and wax esters.Type: ApplicationFiled: March 4, 2013Publication date: January 22, 2015Applicant: Matrix Genetics, LLCInventors: James Roberts, Kimberly Marie Kotovic, Jason W. Hickman, Tracey Jurista, Michael Carleton
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Publication number: 20150024009Abstract: The invention provides an isolated genetically modified non-mammalian organism, wherein the activity of acyl-CoA:sterol acyltransferase/sterol O-acyltransferase (EC 2.3.1.26) and/or diacylglycerol acyltransferase/diacylglycerol O-acyltranferase (EC 2.3.1.20) and/or lecithin cholesterol acyl transferase/phospholipid: diacylglycerol acyltransferase (EC 2.3.1.158) and/or acyl CoA-wax alcohol acyltransferase (EC 2.3.1.75) is reduced or abolished in comparison with a corresponding wildtype organism, methods of use of such an organism, shuttle vehicles for making such an organism and methods for producing such an organism.Type: ApplicationFiled: September 19, 2014Publication date: January 22, 2015Applicant: ORGANOBALANCE GMBHInventors: Christine Lang, Andreas Raab
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Patent number: 8936930Abstract: A method of transforming red microalgae is provided. The method is effected by: (i) culturing red microalgae cells under predetermined light/dilution conditions to thereby generate competent red microalgae cells; and (ii) introducing at least one exogenous polynucleotide into said competent red microalgae cells, thereby transforming the red microalgae.Type: GrantFiled: August 4, 2005Date of Patent: January 20, 2015Assignees: Ben-Gurion University of the Negev Research and Development Authority, Mor Research Applications Ltd.Inventors: Shoshana Arad, Miri Lapidot, Yacob Weinstein, Ron Dagan
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Patent number: 8932576Abstract: Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.Type: GrantFiled: January 27, 2010Date of Patent: January 13, 2015Assignee: The Scripps Research InstituteInventor: Stephen P. Mayfield
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Publication number: 20150010978Abstract: Terpene synthases are enzymes that directly convert IPP & DMAPP to terpenes, such as fusicoccadiene. Described herein are methods and compositions for the production of terpenes and terpenoids for use as fuel molecules or other useful components. Genetically engineered enzymes capable of producing terpenes and terpenoids are also described.Type: ApplicationFiled: August 28, 2014Publication date: January 8, 2015Applicant: Sapphire Energy, Inc.Inventors: NICOLE A HEAPS, Craig A Behnke, David Molina
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Publication number: 20150004704Abstract: The present invention provides methods and compositions for increasing oil content in algae. More particularly, the present invention relates to enhancement of phytohormone activity in algae, by genetic transformation thereof or by supplementing the algal growth medium with phytohormones, to maximize the production of oil within the algae cells.Type: ApplicationFiled: June 30, 2014Publication date: January 1, 2015Inventor: Rudy MAOR
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Patent number: 8921090Abstract: The invention relates to acyl-CoA-independent methods of producing a wax ester in recombinant host cells engineered to express an acyl-ACP wax ester synthase, and an alcohol-forming acyl-ACP reductase. The methods of the invention may take place in photosynthetic microorganisms, and particularly in cyanobacteria. Isolated nucleotide molecules and vectors expressing an acyl-ACP wax ester synthase and/or an alcohol-forming acyl-ACP reductase, recombinant host cells expressing an acyl-ACP wax ester synthase and optionally an alcohol-forming acyl-ACP reductase, and systems for producing a wax ester via an acyl-CoA-independent pathway, are also provided.Type: GrantFiled: March 6, 2012Date of Patent: December 30, 2014Assignee: ExxonMobil Research and Engineering CompanyInventors: Erik Holtzapple, John H. Verruto
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Publication number: 20140377847Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.Type: ApplicationFiled: September 1, 2014Publication date: December 25, 2014Inventors: SCOTT FRANKLIN, ARAVIND SOMANCHI, GEORGE RUDENKO, RIYAZ BHAT, XINHUA ZHAO, RISHA BOND, WALTER RAKITSKY, ALEJANDRO MARANGONI, DIZA BRAKSMAYER
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Publication number: 20140377845Abstract: A method of producing isoprene is disclosed. In one embodiment, the method comprises the steps of obtaining a host transgenic microorganism and observing the production of isoprene by the microorganism. In another embodiment, the invention is a transgenic host microorganism for producing isoprene.Type: ApplicationFiled: August 28, 2014Publication date: December 25, 2014Inventors: Eric L. Singsaas, Amy Wiberley
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Publication number: 20140370563Abstract: The present invention is related to compositions and methods for enhanced synthesis of hydrocarbons, particularly, but not limited to, alkanes. The invention, in one embodiment, utilizes the co-expression of a hydrogen peroxide metabolizing enzyme in the presence of an aldehyde decarbonylase enzyme to relieve hydrogen peroxide inhibition of the aldehyde decarbonylase enzyme by hydrogen peroxide. In a preferred embodiment a catalase-aldehyde decarbonylase expression construct and fusion peptide is used. The present invention also relates to microorganisms engineered to express said enzymes and to produce hydrocarbon molecules.Type: ApplicationFiled: August 24, 2012Publication date: December 18, 2014Applicant: BROOKHAVEN SCIENCE ASSOCIATES, LLCInventors: John Shanklin, Carl Andre
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Publication number: 20140371477Abstract: The present invention relates to recombinant cells, particularly recombinant plant cells, which are capable of producing dihydrosterculic acid and/or derivatives thereof. The present invention also relates to methods of producing oil comprising dihydrosterculic acid and/or derivatives thereof.Type: ApplicationFiled: December 21, 2012Publication date: December 18, 2014Applicants: Commomwealth Scientific and Industrial Research Organisation, Grains Research and Delvelopment CorporationInventors: Craig Christopher Wood, Fatima Naim, Surinder Pal Singh, Shoko Okada
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Publication number: 20140370562Abstract: The present invention provides methods and compositions for producing ?-phellandrene hydrocarbons from cyanobacteria.Type: ApplicationFiled: February 6, 2013Publication date: December 18, 2014Inventors: Anastasios Melis, Fiona K. Bentley, Hsu-Ching Chen Wintz, Andreas Zurbriggen
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Publication number: 20140370564Abstract: The present disclosure generally relates to microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene. Also provided are methods of using the microorganisms in industrial processes including, for use in the production of butadiene and products derived therefrom.Type: ApplicationFiled: December 17, 2012Publication date: December 18, 2014Inventors: Mateus Schreiner Garcez Lopes, Avram Michael Slovic, Iuri Estrada Gouvea, Johana Rincones Perez, Lucas Pedersen Parizzi
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Publication number: 20140370575Abstract: One embodiment of the invention is directed to a genetically enhanced cyanobacterium for the production of a first chemical compound, comprising at least one first recombinant gene encoding a first biocatalyst for the production of the first chemical compound, wherein the gene is under the transcriptional control of a Co2+ or Zn2+-inducible promoter. Such a cyanobacterium can provide a tighter control of the production of the first chemical compound.Type: ApplicationFiled: June 30, 2014Publication date: December 18, 2014Applicant: ALGENOL BIOFUELS INC.Inventors: Ulf Dühring, Kerstin Baier, Frauke Germer, Tuo Shi
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Publication number: 20140370574Abstract: A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zn2+ dependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol. The invention also provides a method for producing the metabolically enhanced cyanobacterium, a method for producing ethanol with the metabolically enhanced cyanobacterium, and a method for screening of alcohol dehydrogenase enzyme expressing cyanobacterial strains for the presence of NADPH-dependent native alcohol dehydrogenase enzymes.Type: ApplicationFiled: June 16, 2014Publication date: December 18, 2014Inventors: Ulf Dühring, Heike Enke, Karl Ziegler, Torsten Schwecke
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Publication number: 20140370595Abstract: The present invention provides methods of producing a product or product precursor of a biosynthetic pathway in a genetically modified host cell. The present invention also provides genetically modified host cells comprising nucleic acids encoding a scaffold polypeptide and nucleic acids comprising nucleotide sequences encoding two or more enzymes in a biosynthetic pathway. The present invention further provides nucleic acids comprising nucleotide sequences encoding scaffold polypeptides, for use in a subject method.Type: ApplicationFiled: June 25, 2014Publication date: December 18, 2014Inventors: John E. Dueber, Jay D. Keasling, Gabriel C. Wu, Ghulam Reza K. Malmirchegini
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Publication number: 20140370603Abstract: Disclosed herein are transgenic cells expressing a heterologous nucleic acid encoding a prephenate dehydrogenase (PDH) protein, a heterologous nucleic acid encoding a homogentisate solanesyl transferase (HST) protein, a heterologous nucleic acid encoding a deoxyxylulose phosphate synthase (DXS) protein, or a combination of two or more thereof. In particular examples, the disclosed transgenic cells have increased plastoquinone levels. Also disclosed are methods of increasing cell growth rates or production of biomass by cultivating transgenic cells expressing a heterologous nucleic acid encoding a PDH protein, a heterologous nucleic acid encoding an HST protein, a heterologous nucleic acid encoding a DXS protein, or a combination of two or more thereof under conditions sufficient to produce cell growth or biomass.Type: ApplicationFiled: June 17, 2014Publication date: December 18, 2014Applicant: Los Alamos National Security, LLCInventors: Richard T. Sayre, Sowmya Subramanian, Edgar Cahoon
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Patent number: 8911966Abstract: The present invention pertains to a method for the efficient production of carotenoids. In particular, the present invention is directed to a method for producing carotenoid and carotenoid containing cells, especially astaxanthin and astaxanthin-containing cells, by generating mutant microorganisms belonging to the photoautotrophic algae of the Class Chlorophyceae and culturing same. The present invention further relates to methods of generating microorganisms producing high yields of carotenoids, in particular astaxanthin, products containing said microorganisms or said carotenoids, and the use of said carotenoids produced by the microorganisms according to the present invention and deposited mutant strains generated from said microorganisms.Type: GrantFiled: February 27, 2013Date of Patent: December 16, 2014Assignee: Cognis IP Management GmbHInventors: Lucia Martin, Irina Obraztsova, Baldomero Cordero, Angeles M. Vargas
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Publication number: 20140363892Abstract: The present application provides novel regulatory elements including promoter sequences from marine microorganisms. The application further discloses DNA constructs containing these novel regulatory elements; transgenic cells, transgenic non-human organisms, and progeny containing these novel regulatory elements. Methods of modifying, producing, and using the regulatory elements are also disclosed. The regulatory elements disclosed herein are particularly suited for use in Nannochloropsis and other microalgae.Type: ApplicationFiled: June 11, 2013Publication date: December 11, 2014Applicant: SYNTHETIC GENOMICS, INC.Inventors: Michele M. Champagne, Toby H. Richardson, Jun Urano
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Patent number: 8889381Abstract: Provided herein are diterpene synthases (diTPS) and methods for producing diterpenoids. Also provided herein are nucleic acid sequences encoding diTPS, diTPS amino acid sequences, diTPS proteins, vectors, cells, transgenic organisms, uses, compositions, methods, processes, and kits thereof.Type: GrantFiled: November 21, 2012Date of Patent: November 18, 2014Assignee: University of British ColumbiaInventors: Joerg Bohlmann, Philipp Zerbe
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Patent number: 8889402Abstract: The invention provides methods of cultivating oil-bearing microbes using xylose alone or in combination with other depolymerized cellulosic material. Also provided are microorganisms comprising an exogenous gene encoding a polysaccharide degrading enzyme, such as a cellulase, a hemicellulase, a pectinase, or a driselase. Some methods of microbial fermentation are provided that comprise the use of xylose and depolymerized cellulosic materials for the production of oil-bearing microorgansims.Type: GrantFiled: October 13, 2011Date of Patent: November 18, 2014Assignee: Solazyme, Inc.Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
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Patent number: 8889401Abstract: The invention provides methods of cultivating oil-bearing microbes using cellulosic material. Also provided are microorganisms containing one or more exogenous genes that facilitate the use of cellulosic materials as a feedstock. Also provided are microorganisms and methods for manufacturing non-alcohol-based fuels and fuel feedstocks through a process of converting cellulosic materials into oils.Type: GrantFiled: April 30, 2010Date of Patent: November 18, 2014Assignee: Solazyme, Inc.Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
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Publication number: 20140335562Abstract: Algae transformed with one or more polynucleotides encoding proteins that confer herbicide resistance are provided. The algae can be grown in small or large scale cultures that include one or more herbicides for the production and isolation of various products.Type: ApplicationFiled: July 11, 2014Publication date: November 13, 2014Applicant: SAPPHIRE ENERGY, INC.Inventors: Su-Chiung FANG, Yan POON, Michael MENDEZ
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Publication number: 20140335579Abstract: The present invention relates to polynucleotides from Cochliobolus heterostrophus C5, Cyanothece sp. CCY0110, Mycocentrospora acerin and Hyaloperonospora parasitica, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.Type: ApplicationFiled: July 23, 2014Publication date: November 13, 2014Inventors: Toralf Senger, Jörg Bauer, Johnathan A. Napier
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Patent number: 8883993Abstract: The present invention provides novel promoter and terminator sequences for use in gene expression in eukaryotic cells, such as algal cells. The invention further provides expression cassettes comprising a promoter, as described herein, operably linked to a gene. The invention further provides expression vectors and host eukaryotic cells, such as algal cells, for expressing a protein encoded by the gene; and methods for stably transforming eukaryotic algae such as Tetraselmis with transgenes.Type: GrantFiled: December 4, 2012Date of Patent: November 11, 2014Assignee: ExxonMobil Research and Engineering CompanyInventors: Jane C. Schneider, Soyan Lieberman, Bo Liu, Eric R. Moellering, John H. Verruto, Amanda Skaggs
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Publication number: 20140328875Abstract: Provided herein are chimeric influenza hemagglutinin (HA) polypeptides, compositions comprising the same, vaccines comprising the same, and methods of their use.Type: ApplicationFiled: December 17, 2013Publication date: November 6, 2014Applicant: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAIInventors: Adolfo Garcia-Sastre, Peter Palese, Florian Krammer
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Publication number: 20140329292Abstract: This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams.Type: ApplicationFiled: September 6, 2013Publication date: November 6, 2014Applicant: DYADIC INTERNATIONAL, INC.Inventors: Mark A. EMARLFARB, Alexander Vasilievich Gusakov, Peter J. Punt, Jan Cornelis Verdoes, Arkady Panteleimonovich Sinitsyn, Elena Vlasenko, Sandra Wihelmina Agnes Hinz, Mark Gosink, Zhijie Jiang
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Publication number: 20140325705Abstract: The present invention provides transgenic plants with altered oil content. The invention further provides nucleic acid sequences and methods for generating such plants.Type: ApplicationFiled: July 15, 2014Publication date: October 30, 2014Inventors: Rudy MAOR, Einat Sitbon, Amir Avniel
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Publication number: 20140322799Abstract: This invention provides a metabolically enhanced cyanobacterium for the production of a chemical compound of interest, having at least two first production genes encoding first biocatalysts for the production of the first chemical compound. One of the two first production genes is under the transcriptional control of a first promoter for the first production gene, whereas the other of the two first production genes is under the transcriptional control of a second promoter for the first production gene. The first promoter and second promoter are separately inducible under different conditions and the at least two first biocatalysts catalyze the same chemical reaction. Metabolically enhanced cyanobacteria according to the present invention allow prolonged production of first chemical compounds.Type: ApplicationFiled: June 30, 2014Publication date: October 30, 2014Applicant: Algenol Biofuels Inc.Inventors: Ulf Dühring, Alexandra FRIEDRICH, Kerstin BAIER, Heike ENKE, Dan KRAMER
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Publication number: 20140315267Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.Type: ApplicationFiled: May 13, 2014Publication date: October 23, 2014Applicant: Solazyme, Inc.Inventors: Scott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walt Rakitsky
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Publication number: 20140315269Abstract: The present invention relates to a method for producing polyphenol compounds, i.e. phloroglucinol or one of its derivatives, with a polyketide synthase of type III (PKSIII) from a brown marine alga. The invention also relates to recombinant nucleic acids coding for a polyketide synthase of type III (PKSIII) from the brown alga Ectocarpus siliculosus (E. siliculosus), to recombinant vectors comprising these nucleic acids, as well as to host cells comprising these vectors. Finally, the invention relates to a method for preparing of various compounds by means of polyphenol compounds produced according to the aforementioned method.Type: ApplicationFiled: September 26, 2012Publication date: October 23, 2014Applicants: UNIVERSITE DE BRETAGNE OCCIDENTALE, UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6)Inventors: Ludovic Delage, Laurence Meslet-Cladiere, Philippe Potin, Sophie Goulitquer