Transformants Patents (Class 435/257.2)
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Publication number: 20130237474Abstract: Modified biosynthetic polypeptide fusion inhibitors, methods for manufacturing, and uses thereof are provided.Type: ApplicationFiled: November 13, 2012Publication date: September 12, 2013Applicant: AMBRX, INC.Inventors: Roberto Mariani, Bruce E. Kimmel
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Patent number: 8530207Abstract: The described invention provides genetically engineered photosynthetic microorganisms expressing prokaryotic acyl-ACP thioesterases and methods of using the genetically engineered photosynthetic microorganisms for producing free fatty acids and/or fatty acid derivatives.Type: GrantFiled: December 13, 2011Date of Patent: September 10, 2013Assignee: ExxonMobil Research and Engineering CompanyInventors: Kevin Watts, Rekha Seshadri, Toby Richardson
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Publication number: 20130224822Abstract: Genetically engineered algae strains for biofuels and bioproduct production have improved iron utilization including iron uptake and storage, and exhibit improved growth characteristics, magnetic separation, and magnetic hysteresis induced cell lysis. Pond production algal strain embodiments with high iron scavenging capabilities limit iron availability to contaminating microorganisms and invading species. Accumulation of high iron and other paramagnetic elements content in the form of ferritin enhances the cells magnetic susceptibility improving efficiency of magnetic separation and magnetic hysteresis induced cell lysis. Several genes of the embodiments are capable of improving iron acquisition including the ferritin gene fer1, iron transport gene fea1, and iron reductase gene fre1.Type: ApplicationFiled: July 7, 2011Publication date: August 29, 2013Inventors: Richard Sayre, Brad Postier
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Publication number: 20130224811Abstract: The invention relates to methods for producing a wax ester in recombinant host cells engineered to express a thioesterase, an acyl-CoA synthetase, an alcohol-forming fatty acyl reductase, and a wax ester synthase. The methods of the invention may take place in photosynthetic microorganisms, and particularly in cyanobacteria. Isolated nucleotide molecules and vectors expressing the thioesterase, acyl-CoA synthetase, alcohol-forming fatty acyl reductase, and wax ester synthase, recombinant host cells expressing the thioesterase, acyl-CoA synthetase, alcohol-forming fatty acyl reductase, and wax ester synthase, and systems for producing a wax ester via a pathway using these four enzymes, are also provided.Type: ApplicationFiled: February 29, 2012Publication date: August 29, 2013Applicant: ExxonMobil Research and Engineering CompanyInventors: Erik Holtzapple, John H. Verruto
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Publication number: 20130224828Abstract: Protein nanoparticles encapsulate cargo proteins within an enclosure containing a protected chemical milieu. Encapsulation within such protected chemical milieu enhances the employability and performance of cargo proteins, particularly cargo enzymes, particularly within otherwise hostile chemical environments. Protein nanoparticles are assembled using shell proteins, such as viral coat proteins like Q?, in the presence of a bifunctional polynucleotide and the selected cargo protein. The bifunctional polynucleotide includes two aptameric activities that assist the disposition and retention of cargo proteins within the protein nanoparticle.Type: ApplicationFiled: November 1, 2011Publication date: August 29, 2013Applicant: QAPSULE TECHNOLOGIES, INC.Inventors: M.G. Finn, Steven Brown, Jason Fiedler
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Publication number: 20130224810Abstract: The present invention provides for the improved production of fatty acid products in a recombinant microorganism or host cell engineered to express a non-native gene encoding a thioesterase and a non-native gene encoding a lysophosphatidic acid acyltransferase (LPAAT), in which the LPAAT is overexpressed in the recombinant microorganism compared to an otherwise identical microorganism that does not include the non-native LPAAT gene. The invention also provides methods of producing fatty acid products using such recombinant microorganisms.Type: ApplicationFiled: February 24, 2012Publication date: August 29, 2013Applicant: ExxonMobil Research and Engineering CompanyInventors: Rekha Seshadri, Jennie Lau Kit, Nicholas Bauman, Robert Christopher Brown
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Publication number: 20130225668Abstract: The invention provides chimeric antigen receptors (CARs) comprising an antigen binding domain of a KDR-1121 or DC101 antibody, an extracellular hinge domain, a T cell receptor transmembrane domain, and an intracellular domain T cell receptor signaling domain. Nucleic acids, recombinant expression vectors, host cells, populations of cells, antibodies, or antigen binding portions thereof, and pharmaceutical compositions relating to the CARs are disclosed. Methods of detecting the presence of cancer in a host and methods of treating or preventing cancer in a host are also disclosed.Type: ApplicationFiled: May 2, 2013Publication date: August 29, 2013Applicant: The United States of America, as represented by the Secretary, Department of Health and HumanInventor: The United States of America, as represented by the Secretary, Department of Health and Human Services
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HYDROCARBON-FORMING OXIDATIVE DECARBONYLASE ENZYME, HYDROCARBONS PRODUCED THEREBY, AND METHOD OF USE
Publication number: 20130217931Abstract: The present disclosure relates to oxidative decarbonylase enzymes, methods of making hydrocarbons with such enzymes, hydrocarbons produced therefrom and uses thereof. More particularly, the present disclosure relates to isolated polypeptide sequences that are cytochrome P450 enzymes with oxidative decarbonylase activity and methods of their use to generate hydrocarbon products, such as biofuels.Type: ApplicationFiled: April 3, 2013Publication date: August 22, 2013Applicant: Board of Regents of the Nevada System of Higher Education, on behalf of the University of NevadaInventors: Claus Tittiger, Gary J. Blomquist -
Publication number: 20130212736Abstract: The present invention is related to biosynthetic oil compositions and methods of making thereof. In some embodiments, the invention relates to the use of endogenous enzymes in plants capable of synthesizing oil. In preferred embodiments, said plants are algae. In further embodiments, said algae are from the family Chlamydomonas, Nannochloropsis, Dunaliella, Chiarella and Scenedesmus. In still further embodiments, said endogenous enzymes are diacylglycerol acyltransferases.Type: ApplicationFiled: December 19, 2012Publication date: August 15, 2013Applicant: Board of Trustees of Michigan State UniversityInventor: Board of Trustees of Michigan State University
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Publication number: 20130210105Abstract: The present disclosure identifies methods and compositions for modifying microbial cells, such that the organisms efficiently synthesize terminal olefins, and in particular the use of such organisms for the commercial production of propylene and related molecules.Type: ApplicationFiled: April 17, 2013Publication date: August 15, 2013Applicant: JOULE UNLIMITED TECHNOLOGIES, INC.Inventor: JOULE UNLIMITED TECHNOLOGIES, INC.
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Publication number: 20130210064Abstract: Disclosed herein are magnetotactic algae, such as algae cells that include magnetic nanoparticles. In some examples the magnetotactic algae express a nucleic acid molecule encoding a bacterial MagA ferrous transporter, a nucleic acid molecule encoding a bacterial Mms6 magnetite binding protein, or both. Also disclosed herein are methods for producing magnetotactic algae and methods of producing biofuel or magnetic nanoparticles utilizing magnetotactic algae. Further disclosed herein are methods of enriching a population of magnetotactic algae cells (for example, increasing the number of magnetotactic algae cells in a population of algae cells). In further embodiments, disclosed herein are methods of selecting a transformed algae cell.Type: ApplicationFiled: June 12, 2012Publication date: August 15, 2013Inventors: Pulak Nath, Scott N. Twary
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Publication number: 20130203132Abstract: Disclosed are the uses of specific genes of the mevalonate and isoprenoid biosynthetic pathways, and of inactive gene sites (the pseudogene) to (1) enhance biosynthesis of isopentenyl diphosphate, dimethylallyl diphosphate and isoprenoid pathway derived products in the plastids of transgenic plants and microalgae, (2) create novel antibiotic resistant transgenic plants and microalgae, and (3) create a novel selection system and/or targeting sites for mediating the insertion of genetic material into plant and microalgae plastids. The specific polynucleotides to be used, solely or in any combination thereof, are publicly available from GeneBank and contain open reading frames having sequences that upon expression will produce active proteins with the following enzyme activities: (a) acetoacetyl CoA thiolase (EC 2.3.1.9), (b) 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase (EC 4.1.3.5), (c) HMG-CoA reductase (EC 1.1.1.34), (d) mevalonate kinase (EC 2.7.1.36), (e) phosphomevalonate kinase (EC 2.7.4.Type: ApplicationFiled: September 4, 2012Publication date: August 8, 2013Inventors: Frederick M. Hahn, Adelheid R. Kuehnle
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Publication number: 20130196394Abstract: A cyanobactenum that produces neutral lipids or alkanes, such as FAME OR FAEE, which may be used for biofuel production.Type: ApplicationFiled: July 1, 2011Publication date: August 1, 2013Applicant: The Arizona Board of Regents for and on Behalf of Arizona State UniversityInventors: Roy Curtiss, III, Xinyao Liu
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Publication number: 20130197247Abstract: Genetically engineered microbial, e.g., Prototheca, cells provide microbial oil useful as a food additive and a source of renewable fuels and industrial chemicals.Type: ApplicationFiled: November 28, 2012Publication date: August 1, 2013Applicant: SOLAZYME, INC.Inventor: Solazyme, Inc.
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Publication number: 20130198900Abstract: The present invention provides bioengineered organisms producing elevated levels of thiamine and/or thiamine derivatives. Particularly, the present invention discloses that modifying TPP-responsive riboswitch results in accumulation of thiamine and/or its derivatives.Type: ApplicationFiled: September 7, 2011Publication date: August 1, 2013Applicants: BEN-GURION UNIVERSITY OF THE NEGEV, YEDA RESEARCH AND DEVELOPMENT CO. LTD.Inventors: Asaph Aharoni, Samuel Bocobza, Michal Shapira
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Publication number: 20130189749Abstract: The present invention provides compositions, methods, and kits comprising PUFAs produced by microalgae, in particular omega-3 and/or omega-6 fatty acids produced by members of the genus Rhodomonas, in particular Rhodomonas salina. The invention also provides compositions, methods, and kits comprising the PUFAs for the prophylactic and/or therapeutic treatment of a disease or condition, in particular a cardiovascular and/or inflammatory disease or condition.Type: ApplicationFiled: December 28, 2012Publication date: July 25, 2013Applicant: WAKE FOREST UNIVERSITY SCHOOL OF MEDICINEInventor: Wake Forest University School of Medicine
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Publication number: 20130189787Abstract: The present invention relates to methods, systems and compositions, including genetically modified microorganisms, directed to achieve decreased microbial conversion of 3-hydroxypropionic acid (3-HP) to aldehydes of 3-HP. In various embodiments this is achieved by disruption of particular aldehyde dehydrogenase genes, including multiple gene deletions. Among the specific nucleic acids that are deleted whereby the desired decreased conversion is achieved are aldA, aldB, puuC), and usg of E. coli. Genetically modified microorganisms so modified are adapted to produce 3-HP, such as by approaches described herein.Type: ApplicationFiled: September 15, 2009Publication date: July 25, 2013Inventors: Michael D. Lynch, Christopher P. Mercogliano, Matthew L. Lipscomb, Tanya E.W. Lipscomb
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Publication number: 20130189751Abstract: The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine.Type: ApplicationFiled: January 9, 2013Publication date: July 25, 2013Applicant: Genomatica, Inc.Inventors: Robert HASELBECK, John D. TRAWICK, Wei NIU, Anthony P. BURGARD
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Publication number: 20130180012Abstract: An isolated protein which is at least partially encoded by a polynucleotide sequence encoding a novel sulfotransferase is provided together with a composition which includes the isolated protein. A transgenic organism transformed by a polynucleotide encoding a protein which is at least partially encoded by a novel sulfotransferase is also provided. The invention also includes a process for in-vivo and in-vitro making a sulfated polysaccharide from an unsulfated polysaccharide or increasing the sulfur content of an already sulfated polysaccharide.Type: ApplicationFiled: April 11, 2011Publication date: July 11, 2013Inventors: Shoshana Arad, Lena Plesser, Yaakov Weinstein
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Publication number: 20130171691Abstract: The present invention pertains to a method for the efficient production of carotenoids. In particular, the present invention is directed to a method for producing carotenoid and carotenoid containing cells, especially astaxanthin and astaxanthin-containing cells, by generating mutant microorganisms belonging to the photoautotrophic algae of the Class Chlorophyceae and culturing same. The present invention further relates to methods of generating microorganisms producing high yields of carotenoids, in particular astaxanthin, products containing said microorganisms or said carotenoids, and the use of said carotenoids produced by the microorganisms according to the present invention and deposited mutant strains generated from said microorganisms.Type: ApplicationFiled: February 27, 2013Publication date: July 4, 2013Applicant: Cognis IP Management GmbHInventor: Cognis IP Management GmbH
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Publication number: 20130164850Abstract: The invention relates to endonucleases cleaving DNA target sequences from algae genomes, to appropriate vectors encoding such endonucleases, to cells or to algae modified by such vectors and to the use of these endonucleases and products derived therefrom for targeted genomic engineering in algae.Type: ApplicationFiled: August 1, 2011Publication date: June 27, 2013Inventor: David Sourdive
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Publication number: 20130164808Abstract: This invention relates to recombinant microorganisms capable of producing isoprene and isoprene production with the use of such recombinant microorganism with good efficiency. In this invention, functional activity of the ispA gene is altered to reduce the production of isoprenoid molecules in recombinant cells engineered to produce isoprene or in cells otherwise susceptible to isoprenoid accumulation during fermentation. This decreased ispA gene functional activity enables enhanced synthesis of isoprene in a host microorganism.Type: ApplicationFiled: December 21, 2012Publication date: June 27, 2013Applicant: DANISCO US INC.Inventor: Danisco US Inc.
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Publication number: 20130164809Abstract: This invention relates to methods for producing isoprene by culturing recombinant cells (e.g., cells engineered to produce isoprene) under reduced oxygen inlet levels.Type: ApplicationFiled: December 21, 2012Publication date: June 27, 2013Applicant: DANISCO US INC.Inventor: DANISCO US INC.
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Patent number: 8465965Abstract: The present invention provides reagents and methods for biomaterial production from cyanobacteria.Type: GrantFiled: June 27, 2008Date of Patent: June 18, 2013Assignee: Arizona Board of RegentsInventors: Hatem Mohammed, Wim Vermaas
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Publication number: 20130143284Abstract: This disclosure describes genetically modified photosynthetic microorganisms, including Cyanobacteria, that contain one or more exogenous genes encoding a diacyglycerol acyltransferase, a phosphatidate phosphatase, and/or an acetyl-CoA carboxylase, and which are capable of producing increased amounts of fatty acids and/or synthesizing triglycerides.Type: ApplicationFiled: February 6, 2013Publication date: June 6, 2013Applicant: MATRIX GENETICS, LLCInventor: MATRIX GENETICS, LLC
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Publication number: 20130143282Abstract: Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.Type: ApplicationFiled: October 19, 2012Publication date: June 6, 2013Applicant: Massachusetts Institute of TechnologyInventor: Massachusetts Institute of Technology
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Publication number: 20130143260Abstract: Provided are an alga in which productivity of a photosynthate is increased, and use of the alga. The alga of the present invention has an increased glutathione concentration in its chloroplast. A method of the present invention for producing biomass is a method for producing biomass with the use of the alga of the present invention or an alga produced by a method of the present invention for producing an alga.Type: ApplicationFiled: August 29, 2011Publication date: June 6, 2013Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Kenichi Ogawa, Masanobu Nishikawa
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Publication number: 20130143306Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms, such that the organisms efficiently convert carbon dioxide and light into compounds such as esters and fatty acids. In certain embodiments, the compounds produced are secreted into the medium used to culture the organisms.Type: ApplicationFiled: February 12, 2013Publication date: June 6, 2013Applicant: Joule Unlimited Technologies, Inc.Inventors: David Arthur Berry, Noubar Boghos Afeyan, Frank Anthony Skraly, Christian Perry Ridley, Dan Eric Robertson, Regina Wilpiszeski, Martha Sholl
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Publication number: 20130143291Abstract: The present invention relates to methods for producing monoterpenes, particularly tricyclene, by culturing microbial organisms that express a terpene synthase and optionally a prenyl transferase.Type: ApplicationFiled: March 30, 2011Publication date: June 6, 2013Applicant: Codexis, Inc.Inventors: Robert McDaniel, Galit Meshulam-Simon, Oscar Alvizo, Xiyun Zhang
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Publication number: 20130129761Abstract: Provided herein are influenza hemagglutinin stem domain polypeptides, compositions comprising the same, vaccines comprising the same and methods of their use.Type: ApplicationFiled: March 30, 2011Publication date: May 23, 2013Applicant: Mount Sinai School of MedicineInventors: Adolfo Garcia-Sastre, Peter Palese, John Steel, Anice C. Lowen
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Patent number: 8445257Abstract: The present invention provides genes, polypeptides and expression constructs therefor, recombinant photosynthetic microorganisms, and method of use thereof, such as for the production of branched-chain alcohols (including 2-methyl-1-butanol, 3-methyl-1-butanol, and isobutanol) and derivatives thereof for a variety of uses.Type: GrantFiled: December 28, 2011Date of Patent: May 21, 2013Assignee: Synthetic Genomics, Inc.Inventors: Paul Gordon Roessler, Bo Liu, Jessica Roxane Kristof
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Patent number: 8445446Abstract: This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.Type: GrantFiled: June 23, 2011Date of Patent: May 21, 2013Assignee: The Scripps Research InstituteInventors: Alexander Deiters, Ashton T. Cropp, Jason W. Chin, Christopher J. Anderson, Peter G. Schultz
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Publication number: 20130122558Abstract: The present invention relates to mutant ?5 desaturases, which have the ability to convert dihomo-?-linolenic acid [DGLA; 20:3 ?-6] to arachidonic acid [ARA; 20:4 ?-6] and/or eicosatetraenoic acid [ETA; 20:4 ?-3] to eicosapentaenoic acid [EPA; 20:5 ?-3] and which possess at least one mutation within the HPGG motif of the cytochrome b5-like domain. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding ?5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant ?5 desaturases in oleaginous yeast, are disclosed.Type: ApplicationFiled: January 8, 2013Publication date: May 16, 2013Applicant: E I DU PONT DE NEMOURS AND COMPANYInventor: E I Du Pont De Nemours And Company
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Publication number: 20130122562Abstract: This invention relates to a recombinant microorganism capable of producing isoprene and isoprene production with the use of such recombinant microorganism with good efficiency. In this invention, the acetoacetyl-CoA synthase gene encoding an enzyme capable of synthesizing acetoacetyl-CoA from malonyl-CoA and acetyl-CoA and one or more genes involved in isoprene biosynthesis that enables synthesis of isoprene from acetoacetyl-CoA are introduced into a host microorganism.Type: ApplicationFiled: August 3, 2012Publication date: May 16, 2013Applicant: Danisco US Inc.Inventors: Ilana S. Aldor, Zachary Q. Beck, Michael C. Miller, Caroline M. Peres
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Publication number: 20130116462Abstract: The present invention relates to novel diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes encoding proteins having diacylglycerol acetyltransferase activity, specifically for transferring an acetyl group to a diacylglycerol substrate to form acetyl-Triacylglycerols (ac-TAGS), for example, a 3-acetyl-1,2-diacyl-sn-glycerol. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms. The present invention also relates to methods of using novel diacylglycerol acyltransferase genes and proteins, including their expression in transgenic organisms at commercially viable levels, for increasing production of 3-acetyl-1,2-diacyl-sn-glycerols in plant oils and altering the composition of oils produced by microorganisms, such as yeast, by increasing ac-TAG production.Type: ApplicationFiled: December 29, 2010Publication date: May 9, 2013Applicant: Board of Trustees of Michigan State UniversityInventors: Timothy Durrett, John Ohlrogge, Michael Pollard
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Publication number: 20130115702Abstract: Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention.Type: ApplicationFiled: January 9, 2013Publication date: May 9, 2013Applicant: PROTERRO, INC.Inventor: PROTERRO, INC.
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Publication number: 20130109098Abstract: Biosecure algae and methods for preparing biosecure algae that have a substantially decreased capability to survive in a natural environment are described. The methods include transforming a genetically modified alga to include an essential gene that is operably linked to a promoter system that is active only in the presence of an inducer compound, transforming the genetically modified alga to include a lethal gene that is operably linked with a promoter system that is inactive only in the presence of a repressor compound. The biosecure algae are only able to survive in an artificial algae culture that includes factors or conditions not found in a natural environment.Type: ApplicationFiled: July 6, 2011Publication date: May 2, 2013Applicant: PHYCAL, INCInventors: F.C. Thomas Allnutt, Bradley Lynn Postier, Richard T. Sayre, Daniel A. Coury, Anil Kumar, Andrew Swanson, Mark Scott Abad, Zoee Gokhale Perrine
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Publication number: 20130102040Abstract: The present disclosure is directed to novel polynucleotide sequences for use in Nannochloropsis gaditana. The novel polynucleotide sequences include control sequences and coding sequences. Also disclosed are novel gene expression constructs wherein N. gaditana promoters/control regions are operatively linked to N. gaditana or non-N. gaditana coding sequences. These novel polynucleotide sequences and expression constructs can be introduced into N. gaditana and can recombine into the N. gaditana genome. Expression from these polynucleotide sequences and expression constructs can enhance N. gaditana biomass and/or lipid biosynthesis. Also disclosed are methods for modifying N. gaditana, for example by stably transforming N. gaditana with nucleic acid sequences, growing the modified N. gaditana, and obtaining biomass and biofuels from the modified N. gaditana.Type: ApplicationFiled: October 17, 2012Publication date: April 25, 2013Applicant: COLORADO SCHOOL OF MINESInventors: Randor Radakovits, Robert Jinkerson, Matthew Posewitz
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Publication number: 20130097733Abstract: The present invention relates to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucletides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.Type: ApplicationFiled: June 21, 2011Publication date: April 18, 2013Applicant: BASF Plant Science Company GmbHInventors: Toralf Senger, Laurent Marty, Sten Stymne, Jenny Lindberg Yilmaz, Johnathan A. Napier, Olga Sayanova, Richard Haslam, Ruiz Lopez Noemi
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Publication number: 20130089906Abstract: The invention provides for methods for the production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in cells via the heterologous expression of phosphoketolase enzymes.Type: ApplicationFiled: October 5, 2012Publication date: April 11, 2013Inventors: Zachary Q. BECK, Andrew C. ELIOT, Caroline M. PERES, Dmitrii V. VAVILINE
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Publication number: 20130089916Abstract: Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.Type: ApplicationFiled: September 26, 2012Publication date: April 11, 2013Applicant: Solazyme, Inc.Inventors: Scott Franklin, Aravind Somanchi, Karen Espina, George Rudenko, Penelope Chua
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Publication number: 20130085110Abstract: The present invention relates to a recombinant factor VIII that includes one or more mutations that result in enhanced stability of both factor VIII and factor VIIIa. Methods of making and using the recombinant factor VIII, and pharmaceutical compositions containing the same are also disclosed. The present invention further relates to an isolated nucleic acid molecule that encodes the recombinant factor VIII, as well as DNA expression systems and host cells containing the isolated nucleic acid molecule.Type: ApplicationFiled: November 30, 2012Publication date: April 4, 2013Applicant: UNIVERSITY OF ROCHESTERInventor: University of Rochester
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Publication number: 20130078678Abstract: Nucleic acids encoding glycosidases useful for the hydrolysis of cellulose and hemicellulose obtained from ciliates residing in a bovine rumen are provided. Also provided recombinant nucleic acids encoding the glycosidases, transformed cells comprising the same and related methods of using the transformed cells and glycosidases to degrade cellulose and/or hemicellulose.Type: ApplicationFiled: September 14, 2012Publication date: March 28, 2013Applicant: THE CURATORS OF THE UNIVERSITY OF MISSOURIInventors: Gary Stacey, Seth D. Findley, Melanie Mormile
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Publication number: 20130078686Abstract: The invention relates to acyl-CoA-independent methods of producing fatty alcohols in recombinant host cells engineered to express an alcohol-forming acyl-ACP reductase. The recombinant host cells may be photosynthetic microorganisms, such as cyanobacteria. Isolated nucleic acid molecules, vectors, and recombinant host cells expressing an alcohol-forming acyl-ACP reductase, and systems for producing fatty alcohols via an acyl-CoA-independent pathway, are also provided. Also provided are microorganisms engineered for the secretion of fatty acids and fatty acid derivatives, including fatty alcohols, and methods of producing fatty acid derivatives using such engineering microorganisms.Type: ApplicationFiled: September 27, 2012Publication date: March 28, 2013Applicant: SYNTHETIC GENOMICS, INC.Inventor: Synthetic Genomics, Inc.
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Publication number: 20130078709Abstract: Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.Type: ApplicationFiled: September 17, 2012Publication date: March 28, 2013Applicant: Solazyme, Inc.Inventors: Scott Franklin, Aravind Somanchi, Karen Espina, George Rudenko, Penelope Chua
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Patent number: 8404466Abstract: One embodiment of the invention provides a metabolically enhanced photoautotrophic, ethanol producing host cell comprising: at least two first metabolic enhancements reducing the enzymatic activity or affinity of at least two endogenous host cell enzymes involved in acetate and lactate fermentation, the first metabolic enhancements resulting in an enhanced level of biosynthesis of acetaldehyde, pyruvate, acetyl-CoA or precursors thereof compared to the respective wild type host cell, at least one second metabolic enhancement different from the first metabolic enhancement comprising an overexpressed enzyme for the formation of ethanol.Type: GrantFiled: February 13, 2012Date of Patent: March 26, 2013Assignee: Algenol Biofuels Inc.Inventors: Kerstin Baier, Ulf Duhring, Christine Oesterhelt, Karl Ziegler, Heike Enke
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Publication number: 20130071895Abstract: A process of producing L-lactate as defined herein by feeding carbon dioxide to a culture of a cyanobacterial cell and subjecting said culture to light, wherein said cell is capable of expressing a nucleic acid molecule, wherein the expression of said nucleic acid molecule confer on the cell the ability to convert a glycolytic intermediate into L-lactate and wherein said nucleic acid molecule is under the control of a regulatory system which responds to light or to a change in the concentration of a nutrient in said culture.Type: ApplicationFiled: April 28, 2010Publication date: March 21, 2013Applicant: PHOTANOL B.V.Inventors: Martijn Bekker, Maarten Joost Teixeira de Mattos, Klaas Jan Hellingwerf
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Publication number: 20130065312Abstract: The present invention provides transgenic algal cells resistant to programmed cell death (PCD) and methods and compositions useful in generating such cells. Specifically, the invention utilizes expression of one or more mammalian anti-apoptotic genes in algal cells to promote resistance to PCD, which is useful for stress tolerance and increased cell viability and biomass production during cultivation.Type: ApplicationFiled: November 3, 2010Publication date: March 14, 2013Applicant: THE JOHNS HOPKINS UNIVERSITYInventors: Michael J. Betenbaugh, George A. Oyler, Julian N. Rosenberg
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Publication number: 20130065314Abstract: One aspect of the invention relates to a method for introducing a cargo molecule into an algal cell, the method comprising preparing a composition comprising the cargo molecule and a cell penetrating peptide (CPP) and exposing the algal cell to the composition. The method may also comprise treating the algal cell to disrupt a cell wall of the algal cell. One aspect of the invention relates to a method for transforming an algal cell, the method comprising preparing a composition comprising a nucleic acid molecule and a cell penetrating peptide and exposing the algal cell to the composition. In some embodiments, the CPP is Transportan, Penetratin, an HIV Tat fragment, or a polyarginine, or a variant thereof.Type: ApplicationFiled: August 24, 2012Publication date: March 14, 2013Applicant: PHYTOCENTRIC BIOTECH INC.Inventor: Trevor Wayne MacMillan
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Publication number: 20130056683Abstract: The invention relates to methods and products for producing fatty acids by manipulating metabolic function in plants and fungus. The fatty acids generated according to the invention may be useful in the production of biofuels.Type: ApplicationFiled: October 6, 2010Publication date: March 7, 2013Applicant: THE REGENTS OF THE UNIVERSITY OF COLORADO, A BODY CORPORATEInventors: Martha Karen Newell, Sandy Berry-Lowe, Richard Tobin, Odbert Triplett