Transformants Patents (Class 435/257.2)
  • CONSTRUCTS AND METHODS FOR INCREASING YIELD OF FATTY ALCOHOLS IN CYANOBACTERIA
    Publication number: 20130059350
    Abstract: A construct containing a gene operably linked to a promoter having activity in cyanobacteria, and vector and cyanobacterium comprising such construct for increasing the yield of fatty alcohols in cyanobacterium is provided.
    Type: Application
    Filed: August 24, 2012
    Publication date: March 7, 2013
    Applicant: SHELL OIL COMPANY
    Inventors: Xuefeng LU, Qianqian GAO, Weihua WANG, Hui ZHAO
  • BIOCATALYTIC OXIDATION PROCESS WITH ALKL GENE PRODUCT
    Publication number: 20130052700
    Abstract: The invention provides a biocatalytic process for oxidation of organic compounds with the aid of an alkL gene product, and microorganisms used in this process.
    Type: Application
    Filed: March 15, 2011
    Publication date: February 28, 2013
    Inventors: Markus Poetter, Andreas Schmid, Bruno Buehler, Hans-Georg Hennemann, Mattijs Kamiel Julsing, Steffen Schaffer, Thomas Haas, Manfred Schrewe, Sjef Cornelissen, Martin Roos, Harald Haeger
  • PROCESS FOR GAMMA-BUTYROLACTONE PRODUCTION
    Publication number: 20130046075
    Abstract: A double-walled plastic jar for cosmetic materials, having an inner compartment to hold the cosmetic, and an annular outer side wall defining the exterior of the jar. The inner compartment carries the lip that seals with a screw cap, and also carries an external skirt, which latter has the screw threads for engagement by the cap. In addition, a weight is disposed in a-concealed manner in the base of the jar, between the walls, to give the jar a heft feel, characteristic of glass or thick plastic walls.
    Type: Application
    Filed: April 4, 2011
    Publication date: February 21, 2013
    Inventors: Johan van Walsem, Erik Anderson, John Licata, Kevin A. Sparks, William R. Farmer, Christopher Mirley, Jeffrey A. Bickmeier, Ann D'Ambruoso, Frank A. Skraly, Thomas M. Ramseier, M.S. Sivasubramanian, Yossef Shabtai
  • ISOPRENE SYNTHASE VARIANTS FOR IMPROVED PRODUCTION OF ISOPRENE
    Publication number: 20130045891
    Abstract: The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved specific productivity. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in host cells.
    Type: Application
    Filed: October 27, 2011
    Publication date: February 21, 2013
    Inventors: ZACHARY Q. BECK, DAVID A. ESTELL, JEFFREY V. MILLER, CHRISTOPHER L. RIFE, DEREK H. WELLS
  • METHODS OF INCREASING PROTEIN, OIL, AND/OR AMINO ACID CONTENT IN A PLANT
    Publication number: 20130045323
    Abstract: The present invention relates to methods for increasing the protein, oil, and/or amino acid content of a plant. The methods involve the manipulation of the expression level of trehalose-6-phosphate synthase (TPS) homologs. Expression cassettes for achieving such gene expression manipulation and transgenic plant cells and plants comprising the constructs and cassettes are also provided. Methods of plant breeding using these plants have also been developed.
    Type: Application
    Filed: August 16, 2012
    Publication date: February 21, 2013
    Applicant: BASF PLANT SCIENCE COMPANY GMBH
    Inventor: Heiko Härtel
  • ANTIGENIC MIMICS OF DISCONTINUOUS EPITOPES OF PATHOGEN RECOGNIZED BY BROADLY NEUTRALIZING ANTIBODIES
    Publication number: 20130039927
    Abstract: The present invention relates to an anti-idiotypic polypeptide scaffold that includes two or more peptide sequences that mimic a discontinuous epitope of a pathogen that is recognized by or induces formation of a broadly neutralizing antibody. Using a fibronectin FNfn10 scaffold bearing two or more modified discontinuous loops, scaffolds that recognize broadly neutralizing antibodies in vitro and from patient serum have been identified. These scaffolds should induce an immune response or mobilize germline specificities to initiate their affinity maturation.
    Type: Application
    Filed: February 14, 2011
    Publication date: February 14, 2013
    Applicant: UNIVERSITY OF ROCHESTER
    Inventors: Stephen Dewhurst, Mark A. Sullivan
  • METHODS AND COMPOSITIONS FOR ENHANCED PRODUCTION OF FATTY ALDEHYDES AND FATTY ALCOHOLS
    Publication number: 20130035513
    Abstract: The invention relates to the use of EntD polypeptides, polynucleotides encoding the same, and homologues thereof to enhance the production of fatty aldehydes and fatty alcohols in a host cell.
    Type: Application
    Filed: January 26, 2012
    Publication date: February 7, 2013
    Applicant: LS9, INC.
    Inventors: Zhihao HU, Derek L. Greenfield, Vikranth Arlagadda
  • ORGANISM WITH ALTERED CAROTENOID CONTENT AND METHOD OF PRODUCING SAME
    Publication number: 20130036518
    Abstract: The present invention relates to means and methods for modifying the expression of a component in the carotenoid biosynthetic pathway to modify the production of carotenoid towards accumulation of at least one of phytoene, phytofluene, zeta carotene or combinations thereof. The present invention further relates to genetically altered organisms having elevated content of these carotenoids. The present invention further relates to genetically altered organisms having elevated content of these carotenoids, particularly in chromoplast-containing cells.
    Type: Application
    Filed: March 9, 2011
    Publication date: February 7, 2013
    Applicant: YISSUM RESSEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM LTD
    Inventors: Joseph Hirschberg, Dani Zamir
  • Mutant ?-5 desaturases mutated in the heme-binding motif (HPGG) and their use in making polyunsaturated fatty acids
    Patent number: 8367383
    Abstract: The present invention relates to mutant ?5 desaturases, which have the ability to convert dihomo-?-linolenic acid [DGLA; 20:3 ?-6] to arachidonic acid [ARA; 20:4 ?-6] and/or eicosatetraenoic acid [ETA; 20:4 ?-3] to eicosapentaenoic acid [EPA; 20:5 ?-3] and which possess at least one mutation within the HPGG motif of the cytochome b5-like domain. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding ?5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant ?5 desaturases in oleaginous yeast, are disclosed.
    Type: Grant
    Filed: August 14, 2012
    Date of Patent: February 5, 2013
    Assignee: E I du Pont de Nemours and Company
    Inventors: Quinn Qun Zhu, Dana M. Walters Pollak
  • Transgenic photosynthetic microorganisms and photobioreactor
    Patent number: 8367379
    Abstract: Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention.
    Type: Grant
    Filed: January 5, 2009
    Date of Patent: February 5, 2013
    Assignee: Proterro, Inc.
    Inventors: John Aikens, Robert J. Turner
  • MATERIALS AND METHODS FOR USING AN ACYL-ACYL CARRIER PROTEIN THIOESTERASE AND MUTANTS AND CHIMERAS THEREOF IN FATTY ACID SYNTHESIS
    Publication number: 20130029387
    Abstract: A method of increasing production of fatty acids comprising introducing into a host cell or organism and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from Bryantella formatexigens or a mutant thereof; a method of making a mutant B. formatexigens acyl-ACP TE; a method of making a chimeric Cuphea viscosissima acyl-ACP TE; a nucleic acid encoding a mutant acyl-ACP TE or a chimeric C. viscosissima acyl-ACP TE; a host cell or organism comprising the nucleic acid; a mutant acyl-ACP TE or chimeric C. viscosissima acyl-ACP TE; a method of altering the specificity of a plant acyl-ACP TE; and a method of altering the level of activity of a plant acyl-ACP TE.
    Type: Application
    Filed: July 25, 2012
    Publication date: January 31, 2013
    Applicant: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
    Inventors: Basil J. Nikolau, Marna Yandeau-Nelson, Fuyuan Jing
  • METHODS AND COMPOSITIONS FOR PRODUCING HYDROCARBONS
    Publication number: 20130029395
    Abstract: Compositions and methods for producing aldehydes, alkanes, and alkenes are described herein. The aldehydes, alkanes, and alkenes can be used in biofuels.
    Type: Application
    Filed: September 24, 2012
    Publication date: January 31, 2013
    Applicant: LS9, INC.
    Inventors: Andreas W. SCHIRMER, Mathew A. Rude, Shane A. Brubaker
  • Enzyme directed oil biosynthesis in microalgae
    Patent number: 8362318
    Abstract: The present invention is related to biosynthetic oil compositions and methods of making thereof. In some embodiments, the invention relates to the use of endogenous enzymes in plants capable of synthesizing oil. In preferred embodiments, said plants are algae. In further embodiments, said algae are from the family Chlamydomonas, Nannochloropsis, Dunaliella, Chlorella and Scenedesmus. In still further embodiments, said endogenous enzymes are diacylglycerol acyltransferases.
    Type: Grant
    Filed: December 16, 2009
    Date of Patent: January 29, 2013
    Assignee: Board of Trustees of Michigan State University
    Inventors: Christoph Benning, Rachel Miller, Eric R. Moellering
  • REGULATION OF TOXIN AND ANTITOXIN GENES FOR BIOLOGICAL CONTAINMENT
    Publication number: 20130023035
    Abstract: The present invention relates to the regulation of a toxin and/or antitoxin genes in a genetically engineered microorganism, such as cyanobacterial or eukaryotic algal strains, in particular for preventing unintentional and/or uncontrolled spread of the microorganisms.
    Type: Application
    Filed: June 28, 2012
    Publication date: January 24, 2013
    Applicant: ExxonMobil Research and Engineering Company
    Inventors: Vincent A. Bielinski, Nicholas Bauman, Kate Lyons Boyd, Srividya Akella, Stanley Bower, Paul G. Roessler
  • DESATURASES OF A GREEN MICROALGA AND USES THEREOF
    Publication number: 20130019341
    Abstract: Isolated proteins which are at least partially encoded by polynucleotide sequences encoding novel desaturases are provided together with a composition which includes these isolated proteins. A transgenic plant, a transgenic alga, or a transgenic seed transformed by the polynucleotides encoding proteins which are at least partially encoded by novel desaturases are also provided. The invention also includes a process for making a very long-chain polyunsaturated fatty acid in a transformed cell, a transgenic alga, or a transgenic plant expressing the isolated protein or proteins which are at least partially encoded by the polynucleotide sequences encoding novel ?5, ?6, or ?12 desaturases.
    Type: Application
    Filed: January 5, 2011
    Publication date: January 17, 2013
    Inventors: Iskandarov Umidjon, Inna Khozin Goldberg, Zvi Hacohen
  • ANTI-KIT ANTIBODIES AND USES THEREOF
    Publication number: 20130011406
    Abstract: Provided herein, in one aspect, are antibodies that immunospecifically bind to a human KIT antigen comprising the fourth and/or fifth extracellular Ig-like domains (that is, D4 and/or D5 domains), polynucleotides comprising nucleotide sequences encoding such antibodies, and expression vectors and host cells for producing such antibodies. The antibodies can inhibit KIT activity, such as ligand-induced receptor phosphorylation. Also provided herein are kits and pharmaceutical compositions comprising antibodies that specifically bind to a KIT antigen, as well as methods of treating or managing a KIT-mediated disorder or disease and methods of diagnosing a KIT-mediated disorder or disease using the antibodies described herein.
    Type: Application
    Filed: March 25, 2011
    Publication date: January 10, 2013
    Applicant: KOLLTAN PHARMACEUTICALS, INC.
    Inventors: Yaron Hadari, Susanne Radke, Joseph Schlessinger, Yoshihisa Suzuki
  • Rice Promoters
    Publication number: 20130007914
    Abstract: The invention provides several promoters isolated from Oryza sativa, which promoters are capable of driving and/or regulating the expression of an operably linked nucleic acid in a plant. The expression patterns of the promoters according to the invention have been studied in Oryza sativa and some of the promoters displayed specific activity in particular cells, tissues or organs of the plant, while others displayed constitutive expression throughout substantially the whole plant. Some promoters showed weak expression, while others were strongly active.
    Type: Application
    Filed: May 15, 2012
    Publication date: January 3, 2013
    Inventors: Yves Hatzfeld, Willem Broekaert
  • ENZYMATIC SYSTEM FOR MONOMER SYNTHESIS
    Publication number: 20130005012
    Abstract: In vivo methods for production of styrene via a recombinant host cell can include cell expression of at least one polynucleotide encoding a polypeptide having cinnamic acid decarboxylase activity, and at least one polynucleotide encoding a polypeptide having phenylalanine ammonia lyase activity.
    Type: Application
    Filed: June 22, 2012
    Publication date: January 3, 2013
    Applicant: PHYTOGENE, INC.
    Inventor: Xiaodan Yu
  • MODULATION OF LOW CARBON DIOXIDE INDUCIBLE PROTEINS (LCI) FOR INCREASED BIOMASS PRODUCTION AND PHOTOSYNTHESIS
    Publication number: 20130007916
    Abstract: The invention provides the disclosure of a novel plant/algae/cyanobacteria photosynthesis, biomass production, and productivity pathway involving low carbon dioxide inducible (LCI) proteins. According to the invention, the activity of one or more LCI proteins may be modulated to increase the same under conditions where such proteins are typically repressed. According to the invention, modulation of LCI protein activity was able to increase biomass production by as much as 80% under elevated CO2 conditions. The invention includes methods, and genetically modified plants/algae/cyanobacteria, cells, plant parts and tissues.
    Type: Application
    Filed: June 28, 2012
    Publication date: January 3, 2013
    Applicant: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
    Inventor: Martin H. Spalding
  • BIOLOGICAL PRODUCTION OF PENTOSE SUGARS USING RECOMBINANT CELLS
    Publication number: 20120329102
    Abstract: The invention provides, inter alia, compositions and methods for the biological production of pentose sugars, such as 2-methylerythritol (2-ME), 1-deoxyxylulose (1-DX), and monoacetylated-2-C-methylerythritols, using recombinant cells.
    Type: Application
    Filed: December 22, 2011
    Publication date: December 27, 2012
    Inventors: Joseph C. MCAULIFFE, Rachel E. MUIR
  • Modified Algae for Improved Biofuel Productivity
    Publication number: 20120329099
    Abstract: Methods, processes, and systems for the production of lipids and starch from modified algae are disclosed. In one embodiment, the modified algae over-expresses isoamylase and accumulates much higher amounts of starch than unmodified algae. In some embodiments, the modified algae comprises one or more copies of an isoamylase expression construct. In one embodiment, the modified algae is a sta7 Chlamydomonas reinhardtii mutant with a starchless phenotype that has been complemented with one or more copies of the wild-type genomic STA7 isoamylase gene construct. The complemented, modified algae accumulates much greater amount of starch than an unmodified algae and may be used to produce large amounts of starch and/or lipids.
    Type: Application
    Filed: March 26, 2012
    Publication date: December 27, 2012
    Applicant: Colorado School of Mines
    Inventors: Matthew C. Posewitz, Randor Radakovits, Robert Jinkerson, Victoria H. Work, Jonathan E. Meuser
  • Genetically Engineered Microorganisms That Metabolize Xylose
    Publication number: 20120329109
    Abstract: The invention provides methods of cultivating oil-bearing microbes using xylose as a fixed carbon source. Also provided are microorganisms that have been genetically engineered to metabolize xylose as a fixed carbon source allowing them to convert xylose into oils. Particular advantages of the processes provided herein include production of oils rather than alcohols through the microbial fermentation processes utilizing xylose.
    Type: Application
    Filed: May 4, 2012
    Publication date: December 27, 2012
    Applicant: Solazyme, Inc.
    Inventors: Penelope Chua, Aravind Somanchi
  • STRESS-INDUCED LIPID TRIGGER
    Publication number: 20120322157
    Abstract: The present disclosure provides novel proteins that when over expressed in algae result in an increase or change in fatty acid and/or glycerol lipid production and/or accumulation, without a substantial decrease in the growth rate of the alga or the break down of algal components, such as chlorophyll. The present disclosure also describes methods of using the novel proteins to increase or change the production and/or accumulation of fatty acids and/or glycerol lipids in algae. In addition, these proteins are useful tools in obtaining information about the fatty acid and triacyglyceride (TAG) synthetic pathways in algae.
    Type: Application
    Filed: February 1, 2011
    Publication date: December 20, 2012
    Applicant: SAPPHIRE ENERGY, INC
    Inventors: Christopher Yohn, April Brand, Michael Mendez, Craig A. Behnke
  • Chimeric Endonucleases and Uses Thereof
    Publication number: 20120324603
    Abstract: The invention relates to chimeric endonucleases, comprising an endonuclease and a heterologous DNA binding domain, as well as methods of targeted integration, targeted deletion or targeted mutation of polynucleotides using chimeric endonucleases.
    Type: Application
    Filed: November 26, 2010
    Publication date: December 20, 2012
    Applicant: BASF Plant Sceience Company GmbH
    Inventors: Andrea Hlubek, Christian Biesgen, Hans Wolfgang Höffken
  • GENETICALLY ENGINEERED HERBICIDE RESISTANT ALGAE
    Publication number: 20120322102
    Abstract: Algae transformed with one or more polynucleotides encoding proteins that confer herbicide resistance are provided. The algae can be grown in small or large scale cultures that include one or more herbicides for the production and isolation of various products.
    Type: Application
    Filed: December 22, 2009
    Publication date: December 20, 2012
    Applicant: SAPPHIRE ENERGY, INC.
    Inventors: Su-Chiung Fang, Yan Poon, Michael Mendez
  • METHODS FOR MICROBIAL PRODUCTION OF TERPENOIDS
    Publication number: 20120315672
    Abstract: The invention relates to recombinant expression of terpenoid synthase enzymes and geranylgeranyl diphosphate synthase (GGPPS) enzymes in cells and the production of diterpenoids.
    Type: Application
    Filed: November 10, 2010
    Publication date: December 13, 2012
    Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Effendi Leonard, Parayil K. Ajikumar, Kristala Lanett Jones Prather, Gregory Stephanopoulos, Kelly Thayer
  • COMBINED USE OF VIP3AB AND CRY1FA FOR MANAGEMENT OF RESISTANT INSECTS
    Publication number: 20120317682
    Abstract: The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising a Vip3Ab insecticidal protein in combination with a Cry 1Fa insecticidal protein to delay or prevent development of resistance by the insect(s).
    Type: Application
    Filed: December 16, 2010
    Publication date: December 13, 2012
    Applicant: Dow Agrosciences LLC
    Inventors: Thomas Meade, Kenneth Narva, Nicholas P. Storer, Joel J. Sheets, Aaron T. Woosley, Stephanie L. Burton
  • COMBINED USE OF CRY1Ca AND CRY1Fa PROTEINS FOR INSECT RESISTANCE MANAGEMENT
    Publication number: 20120317681
    Abstract: The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising a Cry1Fa insecticidal protein and a Cry1Ca insecticidal protein, in combination, to delay or prevent development of resistance by the insect(s).
    Type: Application
    Filed: December 16, 2010
    Publication date: December 13, 2012
    Inventors: Thomas Meade, Kenneth Narva, Nicholas P. Storer, Joel J. Sheets, Aaron T. Woosley, Stephanie L. Burton
  • Production of Therapeutic Proteins in Photosynthetic Organisms
    Publication number: 20120309939
    Abstract: The present disclosure relates to methods of expressing therapeutic proteins in photosynthetic organisms and the therapeutic proteins produced by the methods. The therapeutic proteins include high-mobility group box 1 (HMGB1) protein, fibronectin domain (10) (10FN3), fibronectin domain (14) (14FN3), interferon beta (IFN?), proinsulin and vascular endothelial growth factor (VEGF). The photosynthetic organisms include prokaryotes such as cyanobacteria and eukaryotes such as alga and plants. Transformation of eukaryotes is preferably the plastid genome, more preferably the chloroplast genome.
    Type: Application
    Filed: November 19, 2010
    Publication date: December 6, 2012
    Applicants: THE SCRIPPS RESEARCH INSTITUTE, SAPPHIRE ENERGY, INC.
    Inventors: Beth A. Rasala, Rosa M. F. Cardoso, Machiko Muto, Stephen P. Mayfield, Philip A. Lee, Craig A. Behnke, Michael Mendez
  • COMBINED USE OF CRY1CA AND CRY1AB PROTEINS FOR INSECT RESISTANCE MANAGEMENT
    Publication number: 20120311745
    Abstract: The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising Cry1Ca insecticidal protein and a Cry1Ab insecticidal protein in combination to delay or prevent development of resistance by the insects.
    Type: Application
    Filed: December 16, 2010
    Publication date: December 6, 2012
    Applicant: DOW AGROSCIENCES LLC
    Inventors: Thomas Meade, Aaron T. Woosley, Kenneth Narva, Stephanie L. Burton, Nicholas P. Storer, Joel J. Sheets
  • MUTANT DELTA-5 DESATURASES MUTATED IN THE HEME-BINDING MOTIF (HPGG) AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS
    Publication number: 20120309064
    Abstract: The present invention relates to mutant ?5 desaturases, which have the ability to convert dihomo-?-linolenic acid [DGLA; 20:3 ?-6] to arachidonic acid [ARA; 20:4 ?-6] and/or eicosatetraenoic acid [ETA; 20:4 ?-3] to eicosapentaenoic acid [EPA; 20:5 ?-3] and which possess at least one mutation within the HPGG motif of the cytochome b5-like domain. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding ?5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant ?5 desaturases in oleaginous yeast, are disclosed.
    Type: Application
    Filed: August 14, 2012
    Publication date: December 6, 2012
    Applicant: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Quinn Qun Zhu, Dana M. Walters Pollak
  • TRANSGENIC PHOTOSYNTHETIC MICROORGANISMS AND PHOTOBIOREACTOR
    Publication number: 20120301563
    Abstract: Provided herein are methods of producing and processing a feedstock from a photosynthetic microorganism. Also provided are various transgenic photosynthetic microorganism and photobioreactors for use in the methods.
    Type: Application
    Filed: November 4, 2011
    Publication date: November 29, 2012
    Applicant: PROTERRO, INC.
    Inventors: John Aikens, Robert J. Turner
  • ENDOTOXINS HAVING NEMATOCIDAL ACTIVITY AND METHODS OF USE THEREOF
    Publication number: 20120304337
    Abstract: The invention provides novel ?-endotoxin polypeptides, and variants and fragments thereof, obtained from Bacillus thuringiensis having pesticidal activity against nematodes. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, biopesticide compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant parasitic nematodes.
    Type: Application
    Filed: May 23, 2012
    Publication date: November 29, 2012
    Applicant: Pioneer Hi-Bred International, Inc.
    Inventors: Ute Schellenberger, Jun-Zhi Wei, Genhai Zhu
  • EXPRESSION OF CALEOSIN IN RECOMBINANT OLEAGINOUS MICROORGANISMS TO INCREASE OIL CONTENT THEREIN
    Publication number: 20120301932
    Abstract: Recombinant oleaginous microorganisms having increased oil content due to the expression of a caleosin polypeptide are described. A recombinant oleaginous microorganism of the disclosed invention produces at least 25% of its dry cell weight as oil, and comprises a functional polyunsaturated fatty acid (PUFA) biosynthetic pathway and at least one genetic construct encoding a caleosin polypeptide. A method for increasing the amount of oil in a recombinant oleaginous microorganism is also described.
    Type: Application
    Filed: May 23, 2012
    Publication date: November 29, 2012
    Applicant: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Seung-Pyo Hong, Quinn Qun Zhu
  • ENZYMATIC SYSTEMS FOR CARBON FIXATION AND METHODS OF GENERATING SAME
    Publication number: 20120301947
    Abstract: A system for carbon fixation is provided. The system comprises enzymes which catalyze reactions of a carbon fixation pathway, wherein at least one of the reactions of the carbon fixation pathway is a carboxylation reaction, wherein products of the reactions of the carbon fixation pathway comprise oxaloacetate and malonyl-CoA, wherein an enzyme which performs the carboxylation reaction is selected from the group consisting of phophoenolpyruvate (PEP) carboxlase, pyruvate carboxylase and acetyl-CoA carboxylase and wherein an export product of the carbon fixation pathway is glyoxylate. Additional carbon fixation pathways are also provided and methods of generating same.
    Type: Application
    Filed: February 10, 2011
    Publication date: November 29, 2012
    Applicant: Yeda Research and Development Co. Ltd.
    Inventors: Ron Milo, Arren Bar-Even, Elad Noor
  • Use of genetically modified organisms to generate biomass degrading enzymes
    Patent number: 8318436
    Abstract: The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.
    Type: Grant
    Filed: October 7, 2011
    Date of Patent: November 27, 2012
    Assignees: Sapphire Energy, Inc., The Scripps Research Institute
    Inventors: Stephen Mayfield, Bryan O'Neill, Michael Mendez, Yan Poon
  • PHOTOSYNTHETIC ORGANISMS AND CELLS ADAPTED TO METABOLIZE PHOSPHITE AS A SOURCE OF PHOSPHORUS
    Publication number: 20120295303
    Abstract: System, including methods and compositions, for making and using photosynthetic organisms and cells that are adapted transgenically to metabolize phosphite as a source of phosphorus for supporting growth.
    Type: Application
    Filed: July 27, 2012
    Publication date: November 22, 2012
    Applicant: Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional (CINVESTAV)
    Inventors: Luis Rafael Herrera-Estrella, Damar Lizbeth López-Arredondo, Alfredo Heriberto Herrera-Estrella
  • POLYNUCLEOTIDES ENCODING ISOPRENOID MODIFYING ENZYMES AND METHODS OF USE THEREOF
    Publication number: 20120288905
    Abstract: The present invention provides isolated nucleic acids comprising nucleotide sequences encoding isoprenoid modifying enzymes, as well as recombinant vectors comprising the nucleic acids. The present invention further provides genetically modified host cells comprising a subject nucleic acid or recombinant vector. The present invention further provides a transgenic plant comprising a subject nucleic acid. The present invention further provides methods of producing an isoprenoid compound, the method generally involving culturing a subject genetically modified host cell under conditions that permit synthesis of an isoprenoid compound modifying enzyme encoded by a subject nucleic acid.
    Type: Application
    Filed: March 21, 2012
    Publication date: November 15, 2012
    Inventors: Dae-Kyun Ro, Karyn Newman, Eric M. Paradise, Jay D. Keasling, Mario Ouellet, Rachel a. Krupa, Kimberly Ho, Timothy Ham
  • SALT TOLERANT ORGANISMS
    Publication number: 20120288918
    Abstract: Disclosed herein are transformed non-vascular photosynthetic organisms that are salt tolerant, nucleotides and vectors useful in conducting such transformations, and transformed strains produced by such transformations.
    Type: Application
    Filed: September 15, 2010
    Publication date: November 15, 2012
    Applicant: Sapphire Energy, Inc.
    Inventors: Shane Edward Hopkins, Tanya Joy Shin, Melisa C. Low, Yan Poon, Alvin Aram Cho
  • METHODS AND COMPOSITIONS FOR PRODUCING FATTY ALCOHOLS AND FATTY ALDEHYDES
    Publication number: 20120282663
    Abstract: Compositions and methods for producing hydrocarbons such as aldehydes, alkanes, and alkenes are described herein. Certain hydrocarbons can be used in biofuels.
    Type: Application
    Filed: July 18, 2012
    Publication date: November 8, 2012
    Applicant: LS9, INC.
    Inventors: Andreas Schirmer, Mathew Rude, Shane Brubaker
  • SMOOTHENED POLYPEPTIDES AND METHODS OF USE
    Publication number: 20120282196
    Abstract: Disclosed is an isolated or purified polypeptide or peptidomimetic comprising an amino acid sequence of a portion of a Smoothened (SMO) protein, wherein the portion comprises an amino acid sequence of any of the intracellular loops of the SMO protein, a functional fragment thereof, or a functional variant of either the portion or the functional fragment, wherein the functional fragment comprises at least 7 contiguous amino acids of the intracellular loops, and wherein the functional fragment or functional variant inhibits proliferation of a diseased cell, or a fatty acid derivative thereof. Related conjugates, nucleic acids, recombinant expression vectors, host cells, and pharmaceutical compositions are further provided.
    Type: Application
    Filed: May 14, 2012
    Publication date: November 8, 2012
    Applicant: The USA, as represented by the Secretary, Dept. of Health and Human Services
    Inventors: Nadya Tarasova, Michael Dean, Hong Lou
  • Expanding the Eukaryotic Genetic Code
    Publication number: 20120282689
    Abstract: This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
    Type: Application
    Filed: April 10, 2012
    Publication date: November 8, 2012
    Applicant: The Scripps Research Institute
    Inventors: Jason W. Chin, T. Ashton Cropp, J. Christopher Anderson, Peter G. Schultz
  • COMPOSITIONS AND METHODS FOR THE RAPID BIOSYNTHESIS AND IN VIVO SCREENING OF BIOLOGICALLY RELEVANT PEPTIDES
    Publication number: 20120283136
    Abstract: This invention provides new combinatorial approaches for the biosynthesis and screening of cyclic peptides inside living cells. These novel approaches are useful for finding biologically relevant molecules, e.g., those able to inhibit the cytotoxicity of Anthrax Edema Factor. Key to this ‘living combinatorial’ approach is the use of a living cell as a micro-chemical factory for both synthesis and screening of potential inhibitors for a given molecular recognition event.
    Type: Application
    Filed: June 23, 2010
    Publication date: November 8, 2012
    Inventor: Julio A. Camarero
  • GENETICALLY ENGINEERED CYANOBACTERIA
    Publication number: 20120276637
    Abstract: The disclosed embodiments provide cyanobacteria spp. that have been genetically engineered to have increased production of carbon-based products of interest. These genetically engineered hosts efficiently convert carbon dioxide and light into carbon-based products of interest such as long chained hydrocarbons. Several constructs containing polynucleotides encoding enzymes active in the metabolic pathways of cyanobacteria are disclosed. In many instances, the cyanobacteria strains have been further genetically modified to optimize production of the carbon-based products of interest. The optimization includes both up-regulation and down-regulation of particular genes.
    Type: Application
    Filed: February 24, 2012
    Publication date: November 1, 2012
    Applicant: SOUTH DAKOTA STATE UNIVERSITY
    Inventors: RUANBAO ZHOU, WILLIAM GIBBONS
  • Tailored Oils Produced From Recombinant Oleaginous Microorganisms
    Publication number: 20120277452
    Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
    Type: Application
    Filed: July 12, 2012
    Publication date: November 1, 2012
    Applicant: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walt Rakitsky, Xinhua Zhao, Riyaz Bhat
  • Toxin-Immunity System
    Publication number: 20120270271
    Abstract: The present invention provides host cells whose survivability can be conditionally controlled, and vectors that can be used for preparing such host cells and for selectable cloning.
    Type: Application
    Filed: December 16, 2010
    Publication date: October 25, 2012
    Applicant: UNIVERSITY OF WASHINGTON
    Inventor: Joseph Mougous
  • Rock2 and Rock3, Two New Gain-of-Function Variants of the Cytokinin Receptors AHK2 and AHK3
    Publication number: 20120272409
    Abstract: The present invention relates to two new gain of function variants of the cytokinin receptor proteins AHK2 and AHK3, namely rock2 and rock3, to transgenic organisms comprising at least one of said new gain-of-function cytokinin receptor variants and to a method for the manufacturing of a transgenic plant comprising at least one of the new gain-of-function variants.
    Type: Application
    Filed: July 9, 2010
    Publication date: October 25, 2012
    Inventors: Thomas Schmülling, Tomás Werner, Isabel Bartrina Y Manns, Helen Braun
  • COMPOSITIONS AND METHODS FOR ALTERING ALPHA- AND BETA-TOCOTRIENOL CONTENT
    Publication number: 20120270323
    Abstract: Preparation and use of isolated nucleic acids useful in altering the oil phenotype in plants. Isolated nucleic acids and their encoded polypeptides that alter alpha- and beta-tocotrienol content in seeds and oil obtained from the seeds. Expression cassettes, host cells and transformed plants containing the foregoing nucleic acids.
    Type: Application
    Filed: June 14, 2012
    Publication date: October 25, 2012
    Applicant: E I DU PONT DE NEMOURS AND COMPANY
    Inventor: Knut Meyer
  • METHODS OF MODIFYING LIGNIN BIOSYNTHESIS AND IMPROVING DIGESTIBILITY
    Publication number: 20120272406
    Abstract: The present invention relates to methods for increasing digestibility and/or crude protein of a plant by modifying lignin biosynthesis of the plant. The methods involve the manipulation of the expression level of genes in the lignin biosynthesis pathway by directly reducing the expression of genes using miRNA or using regulation of transcription factors. Expression cassettes for achieving such gene expression manipulation and transgenic plant cells and plants comprising the constructs and cassettes are also provided. Transcription regulating nucleotide sequences for use in the expression cassettes have also been developed as well as methods of using these sequences and cassettes.
    Type: Application
    Filed: March 29, 2012
    Publication date: October 25, 2012
    Applicant: BASF Plant Science Company GmbH
    Inventors: Hanping Guan, Beomseok Seo, Peifeng Ren, David Deppong, Huihua Fu
  • MODIFIED ERYTHROPOIETIN (EPO) POLYPEPTIDES THAT EXHIBIT INCREASED PROTEASE RESISTANCE AND PHARMACEUTICAL COMPOSITIONS THEREOF
    Publication number: 20120264686
    Abstract: Modified erythropoietin (EPO) polypeptides and other modified therapeutic polypeptides are provided. The EPO polypeptides and other therapeutic polypeptides are modified to exhibit physical properties and activities that differ from the unmodified EPO polypeptides and other unmodified therapeutic polypeptides, respectively. Nucleic acid molecules encoding these polypeptides also are provided. Also provided are methods of treatment and diagnosis using the polypeptides.
    Type: Application
    Filed: May 29, 2009
    Publication date: October 18, 2012
    Inventors: Thierry Guyon, Gilles Borrelly, Xavier Gallet, Lila Drittanti, Manuel Vega