Abstract: A method of producing a fertiliser composition, the method comprising: •(a) providing partially decomposed organic matter; •(b) contacting the organic matter with: •(i) an anaerobic digestate; •(ii) a source of nitrate ion; •(iii) a source of ammonia; and •(c) contacting the mixture obtained in step (b) with a source of carbon dioxide. •Also claimed is a fertiliser composition comprising partially decomposed organic matter admixed with an anaerobic digestate, a source of nitrate ion, a source of ammonia and carbon dioxide.

Abstract: A method of treating or remediating contaminated material, such as water or soil, comprises contacting such material with asphaltenes. The asphaltenes are preferably produced as a by-product of petroleum refining and, in particular, a by-product of vacuum residua. An adsorbent material comprising such asphaltenes is also provided.

Abstract: The current invention provides for a microbial consortium comprising one or more ammonium-oxidizing strains which are generally recognized as safe (GRAS) and which are selected from the group of Nitrosomonas, Nitrosospira, Nitrosopumilus, Cenarchaeum, Nitrosoarchaeum, Nitrosocaldus, Caldiarchaeum; and one or more GRAS strains which are commensal to the one or more ammonium-oxidizing strains, and which are selected from the group of Acinetobacter, Alcaligenes, Arthrobacter, Azospirillum, Azotobacter, Bacillus, Beijerinckia, Enterobacter, Erwinia, Flavobacterium, Rhizobium, Serratia and Deinococcus.

Abstract: The present invention relates to animal feed or animal feed additive comprising Bacillus strains which improve the health and performance of production animals. The invention further relates use of the Bacillus strains in animal feed and animal feed additives.

Abstract: An enzymatic processing plant for continuous flow-based enzymatic processing of organic molecules. The enzymatic processing plant including an enzymatic processing area, wherein the enzymatic processing area includes a turbulence-generating pipe with a repeatedly changing centre-line and/or a repeatedly changing cross-section, for generating turbulence to mix a reaction mixture and prevent sedimentation of particles as the reaction mixture is flowing through the turbulence-generating pipe. The enzymatic processing plant and the enzymatic processing area are arranged such that the reaction mixture is subjected to turbulence within the enzymatic processing area for a reaction time of 15 minutes or more.

Abstract: An apparatus for refractory organics conversion into biogas includes an anaerobic digester configured to be fed with organic material comprising refractory organics and to generate biogas and a digestate comprising at least a part of the refractory organics, wherein the apparatus comprises a device for a controlled partial oxidation of the refractory organics comprising an inlet configured to be fed with refractory organics and means configured to control the residence time of the refractory organics within the device for a controlled partial oxidation, so as to partially degrade refractory organics. A method for refractory organics conversion into biogas carried out using such an apparatus is also provided.

Abstract: The subject invention provides microbe-based products and efficient methods of producing them. In specific embodiments, methods are provided for symbiotic cultivation of Acinetobacter venetianus RAG1 and Bacillus subtilis B1, as well as growth by-products thereof. Methods are also provided for using the subject microbe-based products, for example, in microbially enhanced oil recovery (MEOR).

Abstract: Spent stillage remaining after the fermentation of a feedstock for ethanol production may be processed to recover, use, and/or recycle the constituent components of the stillage. Stillage may be mixed, heated, and held at a desired temperature for a period of time. The stillage may then be cooled and treated with an enzyme. The enzymatically treated stillage may be emulsified with oil and water, and then permitted to settle into discrete layers. Individual layers may then be processed.

Abstract: Systems and methods for controlling the temperature and pressure of a cryogenic liquid methane storage unit are provided. The disclosed systems and methods generate methane gas from a reservoir of liquid methane stored within the methane storage unit, vent the methane gas through one or more outlet valves connected to the methane storage unit, and generate electric power using the vented methane gas. The generated electric power can then be used to initiating a cooling cycle, which reduces the temperature of said reservoir of liquid methane and reduces the pressure in said methane storage unit. Micro anaerobic digesters and methane storage units may be configured in a networked environment with a central controller that monitors remote units.

Abstract: A system to process a waste food includes a housing; at least one drum enclosed in the housing where the waste food is mixed up and digested by microorganisms or dehydrated; and at least one cleaner incorporated in or on the machine to permit a jet of water to be turned on and off to clean the drum.

Abstract: This invention refers to a method for preparing a chemical digester characterized by using organic and inorganic elements used for multiple purposes such as an accelerator in the decomposition of organic matter, as a water flocculant, as an organic soil fertilizer and as a means to eliminate the concentration of flies and other inserts in organic matter to decompose. The method comprises heating water in a reactor at a temperature of 38° C., adding a polysaccharide, an anti-thickener and an antifoam. The method then comprises the addition of two organic acids, one of them previously mixed in a second reactor and, finally, an inorganic acid until the mixture is homogenized. Optionally the method comprises the packaging of the mixture and the treatment of organic matter.

Abstract: A computer-implemented method for controlling email distribution list membership in an enterprise email system, including the steps of monitoring and collecting continuously updated information regarding access to email distribution lists of an email system by members of the email distribution lists, ascertaining that a particular member of at least one of the email distribution lists has not accessed the at least one of the email distribution lists for a predetermined period of time, and responsive to the ascertaining, at least one of recommending revoking membership of the particular member to the at least one of the email distribution lists and automatically revoking membership of the particular member to the at least one of the email distribution lists.

Abstract: A sulphur denitrification system includes a liquid input fluidly coupled to a source of saltwater that includes nitrate; a liquid output fluidly coupled to the source of saltwater; a plurality of vertically-oriented tanks, at least one of the tanks including a liquid inlet that is fluidly coupled to the liquid input to receive a flow of the saltwater, a volume configured to enclose a plurality of sulphur particles that support denitrification bacteria that biologically transform the nitrate into at least one of nitrous oxide or nitrogen gas, and a liquid outlet fluidly coupled to the liquid output and the liquid inlets of the tanks; and a circulation system configured to circulate a portion of the saltwater though the liquid input to the liquid inlets of the plurality of tanks, through the plurality of tanks, and from the liquid outlets of the tanks to the liquid output and the liquid inlets of the tanks.

Abstract: In a system and process, sludge is treated by two stages of anaerobic digestion in series separated by intermediate thickening and hydrolysis. The hydrolysis product is transferred to the second digester essentially without dilution.

Abstract: The present invention pertains to a method for isolating nucleic acids from a sample, preferably a blood sample, comprising the following steps: a) obtaining a sample which has been stabilised by the use of at least one cationic detergent, wherein the cationic detergent has formed complexes with the nucleic acids; b) obtaining the complexes optionally together with other sample components from the stabilised sample, wherein said complexes comprise the nucleic acids to be isolated; c) resuspending the complexes and optionally adding one or more additives before, during and/or after resuspension, thereby obtaining a resuspended sample comprising at least i) the nucleic acid to be isolated; ii) at least one chaotropic agent; and iii) at least one chelating agent; and d) isolating nucleic acids from the resuspended sample.

Abstract: The present invention relates to methods and plants for the treatment of an organic waste material, wherein waste is subjected to anaerobic fermentation in a biogas digester; effluent is mechanically separated from the biogas digester into a concentrated fraction and a liquid fraction; the liquid fraction is heated to a high temperature below the boiling point of the liquid; the heated liquid is introduced to a flash column to partially remove volatile carbon dioxide, the pH of the liquid is elevated and ammonia is removed from the liquid.

Abstract: A portable, climate-controlled, continuous flow-through, computer-operated vermiculture bioreactor and method for the treatment of organic solid wastes utilizing accelerated microbiological decomposition including composting and vermicomposting to convert materials into environmentally compatible products, including stable biofertilizer. The portable bioreactor design converts carbon-based organic waste into biofertilizer using thermophilic composting, vermicastings and vermicomposting that can be used directly for plant and farming applications.

Abstract: In a system and process, sludge is treated by two stages of anaerobic digestion in series separated by intermediate thickening and hydrolysis. The hydrolysis product is transferred to the second digester essentially without dilution.

Abstract: A membrane biofilm reactor including a vessel defining a volume is disclosed. The reactor also typically includes a first and second plurality of hollow fiber membranes. Each hollow fiber membrane generally has an outer surface and a lumen, and is located within the volume. The reactor further preferably includes a first and second gas feedstock. The first gas feedstock is provided through a first inlet in fluid communication with the lumens of the first plurality of hollow fiber membranes. The second gas feedstock is provided through a second inlet in fluid communication with the lumens of the second plurality hollow fiber membranes. Finally, the reactor also typically includes a biofilm formed on the outer surface of the hollow fiber membranes and made up of methanotrophs, Methanosarcina acetivorans, or both. The first gas feedstock is preferably different from the second gas feedstock.

Abstract: A method of decontaminating soil and ground water containing organic contaminants and metal compounds. It comprises the steps of first treating such soils and ground water with an effective amount an aqueous solution containing a peroxide and a water soluble chelating agent for a time sufficient to have the water soluble chelating agent chelate at least one of the metals of the metal compounds present in the soil and ground water. Next, the chelated metals are brought into contact with the peroxide to catalytically convert the peroxide to an oxidizing agent and hydroxyl ion. The hydroxyl ion converts the halogenated organic compound into environmentally safe, non-toxic compounds. Gases that are produced from this reaction, and from the oxidation of organic compounds, are used to diffuse solution into the soil to chelate calcium and magnesium, thereby causing the sodium to be dislodged from the soil and reduce sodicity in the soil.

Abstract: A method of treating primary sludge and activated sludge produced by a wastewater system is disclosed. A portion of the activated sludge is wasted to form biological sludge. The biological sludge is thermally hydrolyzed. The method entails cooling the thermally hydrolyzed biological sludge by mixing primary sludge with the thermally hydrolyzed biological sludge. Thereafter, the combined sludge is directed through a pasteurization process and then to an anaerobic digester which performs anaerobic digestion of the combined sludge.

Abstract: The invention provides a method of processing MSW comprising the steps of —providing a stream of unsorted MSW to a microbial fermentation reactor in which the MSW is fermented with agitation at a non-water content of between 10 and 50% by weight and at a temperature of between 35 and 75 degrees for a period of between 1 and 72 hours under conditions sufficient to maintain a live lactic acid bacteria concentration of at least 10.000.000.000 CFU/L, and —removing a stream of fermented unsorted MSW from the reactor and subjecting it to a separation step whereby non-degradable solids are removed to provide a slurry of bio-degradable components. Further, a slurry of bio-degradable components prepared according to said method is provided.

Abstract: The present invention relates to compositions and a process in the field of self-cleaning system using digestive proteins. One composition includes a substrate, a digestive protein capable of decomposing a stain molecule, and a link moiety bound to both said digestive protein and said substrate. An alternative composition includes a digestive protein capable of decomposing a stain molecule and a coating substrate wherein said digestive protein may be dispersed in said coating substrate. The process claim includes binding a substrate to a surface and forming a linker moiety between a digestive protein and said substrate.

Abstract: A process for bio-oxidation of sulfides in mineral ore having a metal such as gold occluded or dispersed within the ore as a sulfide is disclosed. The first step comminutes the ore into particles with a size distribution having a P80 of less than 0.25 inch with minus 106 micron particles in the range of 15% to 40% by weight. Agglomerates are formed by adding to the comminuted ore particles an acidic inoculate solution including water, with the solution further including microorganisms capable of bio-oxidizing the sulfides. The agglomerates are then placed in at least one bio-reactor containment vessel to form an ore bed. The process continues by bio-oxidizing the sulfides in the ore bed; then re-circulating the solution through the ore bed, and continuing the bio-oxidation until a desired bio-oxidation level is achieved. Thereafter, the metal is recovered from the ore.

Abstract: The present invention relates preferably to ways of biologically treating liquids, and devices applicable in this respect. Processes and devices according to the present invention preferably relates to a system for biological treatment of liquid such as water, the system typically comprising a container having first and second gas supplies for creating a first and second circulating flow of liquid and bio-film carriers and preferably also for aerating the liquid. Preferred embodiments of a container according to the invention comprises an outflow channel extending along, and preferably throughout, a perimeter of an upper part of the container, the flow channel having a fluid penetrable wall section facing inwardly so that fluid may flow from the interior of the container, through the fluid penetrable wall section and into the outflow channel, and an outlet for leading fluid out from the outflow channel.

Abstract: The present invention pertains to a method for isolating nucleic acids from a sample, preferably a blood sample, comprising the following steps: a) obtaining a sample which has been stabilized by the use of at least one cationic detergent, wherein the cationic detergent has formed complexes with the nucleic acids; b) obtaining the complexes optionally together with other sample components from the stabilized sample, wherein said complexes comprise the nucleic acids to be isolated; c) resuspending the complexes and optionally adding one or more additives before, during and/or after resuspension, thereby obtaining a resuspended sample comprising at least: i) the nucleic acid to be isolated; ii) at least one chaotropic agent; and iii) at least one chelating agent; and d) isolating nucleic acids from the resuspended sample.

Abstract: Described are methods and systems useful in the processing of food samples to detect pathogenic cells in the samples. Embodiments include homogenizing a food sample, digesting solids of the homogenized food sample, and removing lipids from the homogenized food sample. Cells in the sample can then be concentrated using hollow fiber membrane filtration. Regeneration cycles can enable multiple reuses of the hollow fiber membrane filter. Systems and processes herein can be automated, under the control of a computer.

Abstract: The present invention pertains to a fermentation process for the production of an organic acid salt including the steps of fermenting a microorganism in a fermentation medium in a fermentation reactor to form a fermentation broth having an organic acid salt, wherein part of the organic acid salt is present in the solid state and part of the organic acid salt is dissolved in the fermentation broth; withdrawing at least part of the fermentation broth from the fermentation reactor, providing the broth to a hydrocyclone, and withdrawing a top effluent and a bottom effluent from the hydrocyclone; providing the bottom effluent from the hydrocyclone to a solid/liquid separation step, to form a solid fraction and a liquid fraction, providing at least 30 vol. % of the total of the top effluent from the hydrocyclone and the liquid fraction from the solid-liquid separation step to the fermentation reactor.

Abstract: The present invention provides methods for analyzing large nucleic acids including chromosomes and chromosomal fragments. In one aspect, the present invention provides a method of nucleic acid analysis comprising the steps of (a) obtaining a sample of nucleic acid comprising at least one chromosome or fragment greater than about 1000 base pairs in length and containing a target region; (b) creating an emulsion in which each drop of the emulsion contains an average of between about 0-2, 0-1.75, 0-1.5, 0-1.0, 0-0.75, 0-0.5, or fewer chromosomes or fragments of step (a), (c) performing emulsion PCR, (d) quantifying the number of emulsion droplets containing amplified nucleic acid from the target region; (e) calculating the ratio of droplets containing amplified nucleic acid from the target region to total droplets; and (f) comparing the ratio of step (e) to a reference ratio representing a known genotype.

Abstract: This application discusses, among other things, granular spreaders. In an example, a granular spreader can include a blower configured to provide a flow of air, a metering device configured to provide a flow of granular material to the flow of air to provide a hybrid flow of air and granular material, a plurality of sections configured to release the granular material using the flow of air, wherein each section is configured to release an adjustable portion of the hybrid flow and a controller. The controller can be configured to receive control information for each section, to enable or disable each section responsive to the control information, and to adjust a speed of the blower based on the control information.

Abstract: A method of increasing biogenic production of a combustible gas from a subterranean geologic formation may include extracting formation water from the geologic formation, where the extracted formation water includes at least a first species and a second species of microorganism. The method may also include analyzing the extracted formation water to identify the first species of microorganism that promotes the biogenic production of the combustible gas. An amendment may be introduced to the formation water to promote the growth of the first species of microorganism, and the biological characteristics of the formation water may be altered to decrease a population of the second species in the geologic formation.

Abstract: The present document describes a process and a bioreactor for reducing a bisphenol compound content of a wastewater using a membrane bioreactor containing a biomass in a volatile suspended solid (VSS) form where the biomass in the membrane bioreactor is operating continuously to reduce bisphenol compound content when the bisphenol compound volumetric load is below 108 g m?3 d?1.

Abstract: A method and related apparatus for the removal of excess water from sludge, the method comprising the steps of: (1) fermenting the sludge at a temperature in the mesophilic temperature range, (2) maintaining the fermenting sludge for a predetermined period under a hydraulically quiescent condition to achieve phase separation, (3) separately removing the solid phase and liquid phase and (4) feeding the solid phase to the digester for conversion to biogas.

Abstract: A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a particulate concentration agent that comprises gamma-FeO(OH); (b) providing a fluid sample comprising at least one microorganism strain; and (c) contacting the concentration agent with the sample such that at least a portion of the concentration agent is dispersed in the sample and at least a portion of the at least one microorganism strain is bound to or captured by the concentration agent.

Abstract: A device to produce biogas that includes at least one fermenter (1) to receive a substrate (2) to be percolated, and a percolate container (3). The fermenter (1) and the percolate container (3) are connected with each other such that the percolate within the fermenter (1) seeping through the substrate (2) makes its way into the percolate container (3) which serves as a thermal reservoir, from which it may be directed back to the fermenter (1) as necessary. The percolate container (3) serving as a thermal reservoir and/or the sand trap which serves as a percolate collector (5) are located at least partially below the floor surface (1a) of the fermenter (1).

Abstract: In an ultrapure water producing method, a pre-treatment system treats raw water supplied from a raw water storage tank. A heat exchanger adjusts the treated water to a predetermined temperature before the water is supplied to a first bio-treatment means which is further connected to a second bio-treatment means. The second bio-treatment means is connected to a microbial body separation apparatus and, after treatments are performed in those apparatuses, the result is supplied as treated water to a primary pure water apparatus. Before the second bio-treatment means, a second supply mechanism is provided for adding a nitride source as a nutrient source and oxidizing agent (bactericidal agent). According to the ultrapure water producing method, urea in raw water can be removed to a high degree.

Abstract: A process for stimulating microbial methane production in a petroleum-bearing subterranean formation, comprising: (a) analyzing one or more components of the formation to determine characteristics of the formation environment; (b) detecting the presence of a microbial consortium, comprising at least one methanogenic microorganism, within the formation; (c) assessing whether the formation microorganisms are currently active; (d) determining whether the microbial consortium comprises one or more methanotrophic microorganism; (e) characterization of one or more microorganisms of the consortium, at least one of the members of the consortium being a methanogenic microorganism, and comparing the members of the consortium with at least one known microorganism having one or more known physiological and ecological characteristics; (f) characterization of one or more methanotrophic microorganisms of the consortium (if present), and comparing the members of the consortium with at least one known microorganism having one

Abstract: Systems, compositions, and methods for the bioremediation of a contaminant contained within a contaminated region. The systems, compositions, and methods may include supplying a first bioremediation formulation that includes an ion exchange resin to a first treatment zone that is associated with the contaminated region. The systems, compositions, and methods further may include supplying a second bioremediation formulation that includes a high-mobility oxidant, a low-mobility oxidant, and a nutrient material to a second treatment zone that is associated with the contaminated region. The systems, compositions, and methods also may include a kit of bioremediation formulations that includes the first bioremediation formulation and the second bioremediation formulation and is to be utilized during the bioremediation of the contaminated region.

Abstract: A modified polynucleotide has a different base sequence in at least one codon from a wild-type base sequence encoding a horseradish peroxidase polypeptide. The usage frequency of the modified codon of the polynucleotide corresponds to the codon usage frequencies of three filamentous fungal species in Humicola, Aspergillus, and Trichoderma. The polynucleotide is capable of expressing the polypeptide to be encoded in a filamentous fungus.

Abstract: Disclosed herein is a method for biopile-based bioremediation of hydrocarbon waste with high aromatic content. The method comprises of isolating specific microorganisms and preparing microbial blend, preparing biopile, adding the microbial blend into the biopile, providing nutrient, aeration and watering of biopile.

Abstract: A method for the biological decomposition of organic compounds of the group of hydrocarbons, fats, oils, waxes, and derivatives thereof as well as mixtures thereof in media polluted therewith, in the presence of at least one dispersant, wherein the medium polluted with the pollutants is treated with at least one dispersant and with microorganisms.

Abstract: A method of hydrothermally treating stillage by heating stillage to 200 degrees F. to 350 degrees F., altering physicochemical properties of the stillage, enabling facile separation of the stillage, and creating unique product fractions. A method of performing ethanol fermentation by treating stillage to enable facile separation by heating the stillage to a temperature of 200 degrees F. to 350 degrees F., and separating the treated stillage to recover a high protein solids fraction, a stickwater fraction, and an oil fraction. A method of improving fermentation by heating stillage to a temperature of 200° F. to 350° F. resulting in hydrothermally treated stillage, using all or a portion of the hydrothermally treated stillage as a component of a media, and using the media for a process including fermentation and biomass production. Oil, stickwater, high protein solids fraction, high protein meal, metabolites, biomass, and media obtained from the methods above.

Abstract: The method and apparatus according to the present invention concentrate the formation of the biotechnical product to active site in the reactor, where the production is accelerated to extreme speeds by altering the conditions. To achieve this, anabolic, catabolic or overflow metabolic reactions can be utilized. The aim is to implement production so that carbon dioxide emissions in particular are minimized The products are e.g. 2,3-butanediol, butanol, ethanol, acetone, organic acids, methane or hydrogen gas and other fuels or compounds necessary for chemical or material production.

Abstract: The present invention relates to a method of selectively obtaining phosphorus from solids containing heavy metals and phosphate. In this method, the solid is treated under acidic aerobic conditions using microorganisms comprising leaching microorganisms and polyphosphate-accumulating microorganisms, so that the heavy metals and the phosphates are released from the solid and the released phosphates may be taken up by the polyphosphate-accumulating microorganisms. The phosphorus-enriched biomass that is obtained in this manner is separated and may be utilized, e.g., as organic fertilizer.

Abstract: Methods for producing biofuels that involve enhanced separation of oil in co-product process streams are disclosed. In some embodiments, separation is enhanced by addition of an additive such as a surfactant, yeast and/or salt to the co-product process stream.

Abstract: A method of producing sulfur-free nanoparticles involves growing yeast in a growth medium containing a source of an element in a bio-reducible oxidation state (e.g. Se(Vl), and, precipitating nanoparticles containing the element in a lower oxidation state (e.g. Se(O)) than the oxidation state of the element in the source. The method advantageously can provide substantially spherical nanoparticles at high production efficiencies.

Abstract: The present technology provides for enzyme compositions with enhanced enzyme activity, thermophilic and psychrophilic stability. Additionally, the present technology provides for methods and kits for making and using the enzyme compositions.

Abstract: A zero-discharge landfill process is disclosed herein. The process described in the present invention recycles the exhaust gases from a combustion engine which reheats the landfill and provides moisture. Additionally, the CO2 from the exhaust gases releases additional methane. Methane production in landfills can thus be enhanced by the method of the present invention. Furthermore, a portion of the exhaust gas can be used to cultivate algae in a cultivation tank. The cultivation of the algal species is enhanced in the presence of water, CO2, CO, and the elevated temperatures. Optionally, a first portion of the cultivated algae can be passed through the landfill to increase methane production in the landfill and a second portion of the cultivated algae can be transferred to a biodiesel production tank, for the conversion of the one or more algal oils and lipids to a biodiesel or a biofuel by a transesterification reaction.

Abstract: A method for degumming an oil composition comprises the steps of (a) providing an oil composition containing a quantity of phospholipids, (b) contacting said oil composition simultaneously with one or more phospholipase A enzymes and one or more phospholipase C enzymes, under conditions sufficient for the enzymes to react with the phospholipids to create phospholipid reaction products, and (c) separating the phospholipids reaction products from the oil composition, the remaining oil composition after the separation being a degummed oil composition, whereby during step (b) the reaction of said one or more phospholipase A enzymes proceeds at a faster rate than it would in the absence of said one or more phospholipase C enzymes.

Abstract: Hybrid nuclease molecules and methods for treating an immune-related disease or disorder in a mammal, and a pharmaceutical composition for treating an immune-related disease in a mammal.