Abstract: Provided herein is a method for incubating living cells, in accordance with principles of the disclosure, may include the steps of: (a) providing a pressurized gas to a medium reservoir, the pressurized gas providing an impetus that moves a growth medium in the medium reservoir to a bioreactor chamber via a first incoming fluid line connecting the medium reservoir to the bioreactor chamber; (b) simultaneously or subsequently to step (a), providing a pressurized gas to a cell reservoir holding a suspension of the living cells, the pressurized gas providing an impetus that moves the suspension to the bioreactor chamber via a second incoming fluid line connecting the cell reservoir to the bioreactor chamber; and (c) incubating the growth medium and the living cells in the bioreactor chamber, under conditions compatible with cell viability.

Abstract: We describe apparatuses, systems, method, reagents, and kits for conducting assays as well as process for their preparation. They are particularly well suited for conducting automated sampling, sample preparation, and analysis in a multi-well plate assay format. For example, they may be used for automated analysis of particulates in air and/or liquid samples derived therefrom in environmental monitoring.

Abstract: Disclosed herein are methods of utilizing machine learning methods to analyze microscope images of populations of cells.

Abstract: A method and apparatus for automatically transferring sections from a sample block to a tape and for transferring select sections to a slide. A tissue storage system for storing cut sections on the tape and digital storage system for storing photos and other information during the transfer of the sections can also be provided, thereby creating a tissue repository and data repository for future use.

Abstract: A beverage order fulfillment system allows for beverage fulfillment from a mobile device without requiring a custom software application on the mobile device but uses a web connection to control beverage dispenser operations instead. The mobile device incorporates web browser software, and the web browser software is configured to initiate communication sessions with the at least one server. Data entry options are displayed on the interactive GUI for the first computer to communicate beverage selection commands to the beverage dispensing software, via the at least one server, in a respective instance of beverage fulfillment. The at least one server incorporates web socket application program interfaces (APIs) stored on the at least one server and configured to connect a communications link to the beverage dispenser and the web browser software on the first computer.

Abstract: A nucleic acid extraction system, comprising: a nucleic acid extraction plate, and a pipetting device and positive pressure device that are arranged side by side, the pipetting device comprising: an infusion device, an infusion device mounting frame, an infusion device driving unit, and a plurality of infusion units, each of the infusion units comprising: a reagent supply device and a reagent flow controller; the positive pressure device comprises: a plug, an air pipe, a positive pressure provider and a positive pressure driving device. The nucleic acid extraction system is highly efficiency and low-cost when extracting nucleic acid.

Abstract: A sample analyzer includes a suction unit configured to suction a sample in a sample container through a stopper installed in an opening of the sample container; a rack transport unit configured to transport a sample rack holding a sample container along a transport path, and position the sample container held by the sample rack at a suction position by the suction unit; a sample transport unit in which a sample container other than the sample container transported by the rack transport unit is installed and which is configured to transport the installed sample container to the suction position provided on the transport path; a measurement unit configured to measure a sample suctioned by the suction unit from the sample container positioned at the suction position; and an analysis unit configured to analyze the sample based on the measurement result of the measurement unit.

Abstract: A culturing assistance device includes an image acquisition unit configured to acquire a captured image of cells during culturing at determined times, a storage control unit configured to store the captured image acquired by the image acquisition unit and event information indicating an event related to culturing of the cells, and a learning unit configured to learn a relationship between the stored captured image and the stored event information.

Abstract: The invention relates to a method for the preparation of living, microbial samples and microorganisms for subsequent mass spectrometric measurement and evaluation. Findings which can be derived from such a measurement can particularly serve the faster identification of microorganisms in the microbial sample according to species/subspecies and/or the fast determination of resistance/sensitivity of the microorganisms to antimicrobial substances and/or the further characterization of microorganisms, for example in respect of pathogenicity, virulence and metabolism. According to a preferred embodiment of the invention, the preparation particularly takes place directly on a mass spectrometric sample support.

Abstract: A cell treatment system includes an analysis unit to calculate the area or size of each of cell aggregates existing on the cell culture vessel based on an image; a display device to show the area or size of each of the cell aggregates on a display; a laser irradiator to emit laser toward the cell culture vessel so as to kill cells existing on the cell culture vessel; an input device to receive an operation input designating cells to be killed with irradiation of the laser or cells to survive without the irradiation of the laser among the cells existing on the cell culture vessel, or an operation input designating a position to be irradiated with the laser or a position not to be irradiated with the laser on the cell culture vessel; and a control unit to control a position irradiated with the laser.

Abstract: A method of storing digital data in non-biological sequence-controlled polymers includes converting a digital data file into a monomer sequence, synthesizing polymer chains according to the monomer sequence, and encapsulating the polymer chains into microfluidic droplets and providing the microfluidic droplets with addresses. A polymer data storage system has a first converter to convert digital data to a polymer sequence, a polymer synthesizer to produce polymer chains according to a pre-determined monomer sequence, a fluidic encapsulation system to encapsulate the polymer chains in microfluidic droplets and to apply addressing materials to the microfluidic droplets, a storage for storing the microfluidic droplets, a droplet sorting system having at least an actuator to sort the droplets, a sequencer to derive the polymer sequence from the polymer chains contained in the droplets, and a second converter to convert the polymer sequence to digital data.

Abstract: A thermal block assembly is provided. The assembly can comprise a substrate comprising a first surface configured with a plurality of reaction sites each reaction site configured to contain a biological sample and a sample block comprising a plurality of pedestals configured to thermally modulate the plurality of biological samples wherein each pedestal is thermally coupled to one of the reaction sites. The assembly can further comprise cooling blocks, slots and insulating rings associated with reaction sites each capable of minimizing heat flow between reaction sites. A method for thermally isolating reaction sites is also provided.

Abstract: The present invention relates to the automated maintenance of cells particularly reprogrammed somatic cells into iPSCs to enable the large-scale culture and passaging of human pluripotent stem cells (PSCs) that can be adapted to a Freedom EVO®.

Abstract: A production management system includes a storage unit and a controller. The storage unit is configured to store a holding position at which a first work is held among holding positions of the holding unit including first and second cases respectively capable of holding a plurality of works and a work ID identifying the first work in such a manner that the holding position and the work ID of the first work are associated each other. The controller configured to change information of the holding position associated with the work ID of the first work in the storage unit from a first position to a second position if, within the first case, the first work held at the first position is held anew in the second position different from the first position.

Abstract: Methods and devices are provided for simultaneous irradiation of a target, such as mammalian cells and/or nanostructures. The target is simultaneously exposed to optical irradiation and oscillating magnetic field radiation. The simultaneous optical irradiation and oscillating magnetic field radiation occur within an incubator-actuator device. The incubator-actuator device includes a sample chamber, a magnetic field generating coil, and, a light-emitting diode (LED) placement cage.

Abstract: Aspects relate to automated cell culture incubators comprising imaging systems, e.g., holographic or phase-contrast imagers, configured for imaging said cells within the incubators.

Abstract: A method of killing specific cells from among a group of cells cultured in a culture vessel by quick and brief laser treatment, the cell culture vessel comprising a main body and a to-be-irradiated layer attached to the main body, the to-be-irradiated layer containing an ingredient capable of absorbing laser light upon laser irradiation, the group of cells being cultured on the surface of the to-be-irradiated layer, the method comprising: applying laser light to a partial area of the to-be-irradiated layer directly below the specific cells.

Abstract: The present invention relates to a detection mechanism for polymerase chain reaction and a polymerase chain reaction device, wherein the detection mechanism comprises at least one excitation module group, each of the excitation module groups comprising two excitation modules for providing excitation light with two wavelengths; an excitation optical fiber, connected to the excitation modules, the excitation optical fiber transmitting the excitation light to at least one reaction tube, each of the reaction tubes receiving excitation light with two wavelengths; a receiving optical fiber, for collecting and transmitting a fluorescent signal from the reaction tube; at least one receiving module group, connected to the receiving optical fiber, each of the receiving module groups comprising two receiving modules, to respectively receive the fluorescent signal of two wavelengths from the same said reaction tube, and convert the fluorescent signal into an electrical signal for output; the detection mechanism is config

Abstract: Provided are a cell dispensing and storing apparatus and a method of the same. The method includes: moving a freezing container with a test tube in it and a temporary storage bottle containing sample cells into a dispensing area. Connect the temporary storage bottle with an injecting assembly. A dispensing clamp moves the test tube from the freezing container to a tube rack on a rotating platform. A rotating clamp removes a tube cover from the test tube. An injecting nozzle extracts the sample cells from the temporary storage bottle and dispenses the sample cells into the test tube. The rotating clamp puts the tube cover back on the test tube. The dispensing clamp moves the test tube back into the freezing container. A freezing clamp moves the freezing container into a freezer.

Abstract: A cell culture evaluation system includes a cell culture device 10 culturing a plurality of cell culture units of which arrangements are changed; and a cell quality evaluation device 60 including a cell quality evaluation portion 61 that performs image-evaluation on qualities based on images of the cell culture units and a cell arrangement-position storage unit 64 that stores respective arrangement-positions of the cell culture units before and after the arrangement change. When portion of the cell culture units among the plurality of cell culture units are selected to be subjected to image-evaluation, if cell culture units of which results of the image-evaluation do not satisfy predetermined conditions exist, cell culture units, which have been arranged in a predetermined distance range from the cell culture units that do not satisfy the conditions, are specified and evaluated to a point of time of the image-evaluation based on the stored respective arrangement-positions.

Abstract: A component feeder including a stationary picking surface for receiving components to be picked, a vision system including an image unit arranged to take images of components distributed on the picking surface and a load device including one or more load sensors adapted to detect the presence of a component on the picking surface and the vision system is configured to automatically trigger the image unit to take an image upon detecting the presence of the component on the picking surface.

Abstract: A thermal cycler system for use with a sample holder configured to receive a plurality of samples includes a sample block configured to receive the sample holder, a cover lid configured to move in a direction toward the sample block from an open position to a closed position, a heated cover operatively coupled to the cover lid and configured to move in a direction toward the sample block from a raised position to a first lowered position, in which the heated cover contacts the sample holder when the sample holder is received by the sample block, and a drive assembly including a motion guide configured to move in a direction toward the sample block from a first position, wherein the cover lid is in the open position and the heated cover is in the raised position, to a second position, wherein the cover lid is in the closed position.

Abstract: The invention is a method for estimating the amount of analyte in a fluid sample, and in particular in a bodily fluid. The sample is mixed with a reagent able to form a color indicator in the presence of the analyte. The sample is then illuminated by a light beam produced by a light source; an image sensor forms an image of the beam transmitted by the sample, from which image a concentration of the analyte in the fluid is estimated. The method is intended to be implemented in compact analyzing systems. One targeted application is the determination of the glucose concentration in blood.

Abstract: The invention herein relates to conducting assays with an apparatus including a substantially transparent assay cartridge loaded with magnetic beads, and a magnet carrier base positioned below a scanning platform holding the assay cartridge. The assay cartridge includes magnetic beads, sample and control solutions in some wells, and assay reagents in others. A microcomputer controls a stepping motor which controls movement of the magnet carrier base, and causes the magnetic beads to travel from one well to another. An electromagnetic coil-spring assembly induces mixing of well contents with the magnetic beads on actuation. The assay cartridge is authenticated by sending its encoded identifier to a server or website, and assay instructions are provided remotely to the microcomputer. Following assay completion, the cartridge can have color change or other assay indication detected, and the results sent to the server or website or another recipient.

Abstract: The invention is a method for estimating the amount of analyte in a fluid sample, and in particular in a bodily fluid. The sample is mixed with a reagent able to form a color indicator in the presence of the analyte. The sample is then illuminated by a light beam produced by a light source; an image sensor forms an image of the beam transmitted by the sample, from which image a concentration of the analyte in the fluid is estimated. The method is intended to be implemented in compact analyzing systems. One targeted application is the determination of the glucose concentration in blood.

Abstract: When taking in outside air with a fan to control the temperature inside a covered portion where a specimen is installed, the internal temperature may change depending on an environment temperature to cause a difference in temperature control. The wind generated by the fan may blow against a reaction container depending on its loading position and it may influence the temperature control over individual specimens and lead to problems with temperature accuracy, temperature rise speed, and temperature drop speed. A reaction container avoiding these problems has a covered portion which performs temperature control on the reaction container and in which a cover and a fin cover have a heat insulating structure. A heat source is provided for controlling an internal temperature of an internal covered space. The internal temperature is kept constant and the influence of the environment temperature over the temperature control of the reaction container is minimized.

Abstract: The present invention includes a conveyor body which partitions a plurality of storage chambers, a connection opening connected to an external device, and a conveying chamber that connects the plurality of storage chambers to the connection opening, a plurality of incubators detachably stored in the plurality of incubators, a conveyor device that conveys one of the plurality of incubators from the storage chamber to the connection opening, and an environment control device that individually controls an interior environment of the plurality of incubators by supplying and discharging gas to and from each incubator.

Abstract: A cell culture apparatus comprises at least one storage space, at least one barrier, a working space, a sterilization space, and a control space. The at least one storage space comprises a container shelf, an upright track, a rotatable holder, and a light tube. The working space comprises a filling station and an inspecting station. The filling station comprises a first delivering device, an open-close device, and a filling mechanism. The inspecting station comprises a first inspecting mechanism and a second delivering device. Also provided is a method of using the cell culture apparatus including steps of filling, inspecting, or collecting cell cultures, therefore, the cell culture apparatus performs multiple cell culture processes automatically.

Abstract: A microplate-based apparatus for measuring cell metabolism is provided. The apparatus is capable of providing automation of all procedures, ranging from drug injection into each well of a microplate to the measurement of dissolved-oxygen (DO) concentration and hydrogen ion concentration.

Abstract: The automated cell culture arrangement according to the invention comprises at least one closed cell culture module with at least one bioreactor. The closed cell culture module is a closed system, which means that within the closed cell culture module a closed sterile environment can be maintained. The automated cell culture arrangement according to the invention, further comprises at least one pump for pumping liquids within the closed cell culture module and at least one additional tool module, which is configured or configurable to act upon or to monitor the contents of a bioreactor and is movable relative to the at least one closed cell culture module or it is movable relative to one or several components of the at least one closed cell culture module.

Abstract: A method for the detection of microorganisms in a sample comprising contacting said sample with a biosensor concentration module, allowing microorganisms to grow for a first period of time and detecting growth of discrete microorganisms as an indication of the presence of said microorganisms.

Abstract: A method of processing liquid biological material includes providing a liquid processing system. The liquid processing system includes a trunk, a first arm, a second arm, a driving mechanism, and physiochemical equipment. The first arm includes a first robot hand. The second arm includes a second robot hand. A process is performed on a cell suspension in which periphytic cultured cells are suspended as a liquid biological material. In the process, a culture vessel is held with the first robot hand in a state with an open top of the culture vessel facing upwards. The culture vessel has a culture face where the cultured cells are cultured on a base thereof. A cell scraper is held with the second robot hand. The cell scraper is provided with a blade to scrape the cultured cells from the culture face.

Abstract: A method of determining the presence or absence in a sample of a biomarker indicative of exposure to mycobacteria, the method comprising: (a) providing a substrate carrying a mycolic acid derived antigen; (b) contacting the substrate with the sample; (c) contacting the substrate with a fluorophore species; (d) creating an evanescent wave at the boundary of the substrate and the sample; (e) detecting the presence or absence of fluorescence.

Abstract: A thermal cycler includes an attachment unit having an insertion opening for insertion of a reaction container including a channel filled with a reaction solution containing reverse transcriptase enzyme and a liquid having a lower specific gravity than that of the reaction solution and being immiscible with the reaction solution, a first heating unit that heats a first region of the channel, a second heating unit that heats a second region of the channel nearer the insertion opening than the first region, a drive mechanism that switches arrangement of the attachment unit, the first heating unit, and the second heating unit between a first arrangement and a second arrangement, and a control unit that controls the first heating unit to be at a temperature at which reverse transcription reaction progresses and the second heating unit to be at a temperature at which the reverse transcriptase enzyme is not deactivated.

Abstract: A culture device containing a culture vessel for culturing a cell, in which the culture device includes a thermostatic vessel having a stacker containing a plurality of culture vessels for culturing a cell, and an observation portion arranged to be isolated from an atmosphere at inside of the thermostatic vessel and having an object lens, an object lens drive portion, and a camera portion to observe a cell at inside of the culture vessel installed at an observation position at inside of the thermostatic vessel.

Abstract: A transfer device for a culture vessel for transferring a culture vessel for culturing a cell, a culture device including the transfer device for a culture vessel, and a holder for a culture vessel for holding a culture vessel, in which the transfer device for a culture vessel includes a transferring unit transferring a culture vessel for culturing a cell, an inputting unit inputting a kind of the culture vessel, a speed setting unit setting a transfer speed of the culture vessel based on a kind of the culture vessel inputted to the inputting unit, and a controlling unit controlling a transfer speed of the transferring unit to be the transfer speed set by the speed setting unit.

Abstract: Systems and methods of sample processing and temperature control are disclosed. The invention may especially relate to temperature control, and may in some embodiments be methods of temperature control of an automated sample processing system and methods of automated sample processing. Specifically, the present invention provides temperature control in relation to sample processing systems and methods of processing samples, and in some embodiments provides temperature control in relation to sample carriers and processing materials such as reagents. Corresponding systems and devices are disclosed, including sample processing systems (1), sample carrier temperature regulation systems (60), reagent temperature regulation systems, sample processing control systems, and temperature regulation devices, among other embodiments.

Abstract: The present invention provides a method and system for using eye-safe infrared energy from a Class I laser to manipulate cells in culture. The laser energy produces one or more phase boundary propulsion events, which generate hydrodynamic forces sufficient to manipulate cells at the focal point.

Abstract: A device is provided for use with a tissue penetrating system and/or a metering device for measuring analyte levels. The device comprises a cartridge and a plurality of analyte detecting members mounted on the cartridge. The cartridge may have a radial disc shape. The cartridge may also be sized to fit within the metering device. The analyte detecting members may be optical system using fluorescence lifetime to determine analyte levels. In one embodiment, the device may also include a fluid spreader positioned over at least a portion of the analyte detecting member to urge fluid toward one of the detecting members. A plurality of analyte detecting members may be used. Each analyte detecting member may be a low volume device.

Abstract: The invention provides methods of performing a sizing analysis. In the methods, a sizing ladder used in performing the sizing analysis is corrected. In one method, the sizing ladder is corrected for batch-to-batch variations in a sieving gel. In another method, the sizing ladder is corrected for a sample concentration that is different from the archival sizing ladder concentration. Methods are also provided in which the sizing ladder is corrected using a standard marker in a sample and/or using a real-time standard sizing ladder. The methods may be used individually or in combination.

Abstract: An attachment unit for attachment of a reaction container including a channel filled with a reaction solution and a liquid having a specific gravity different from that of the reaction solution and being immiscible with the reaction solution, the reaction solution moving close to opposed inner walls, a first heating unit that heats a first region of the channel and a second heating unit that heats a second region of the channel when the reaction container is attached to the attachment unit, a drive mechanism that switches arrangement of the attachment unit, the first heating unit, and the second heating unit between a first arrangement and a second arrangement in which a lowermost position of the channel is located within a first region and a second region, respectively, and a control unit that controls the drive mechanism, the first heating unit, and the second heating unit are provided.

Abstract: A method and apparatus for locating and selecting a colony of microorganisms on a culture dish and identifying microorganisms in said selected colony using MALDI. The method comprises the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of said selected colony of microorganisms; depositing at least some of said sample of said selected colony of microorganisms on a target plate; and transferring said target plate with said sample in an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. A sample of a colony of microorganisms is automatically deposited on a depositing spot such that the sample covers at most approximately half of said one of the depositing spots of the target plate.

Abstract: Provided are incubation systems for incubating samples for an assay. In certain aspects, the systems include a rotary platform, a sample container positioned at a peripheral edge of the platform, a plurality of activity sites including at least a first and a second activity site, a processor, and a memory that includes instructions. When executed by the processor, the instructions cause the system to rotate the rotary platform to move the sample container from the first activity site to the second activity site, extract a first portion of the sample from the sample container at the first activity site for a measurement based on a first incubation time period, and extract a second portion of the sample from the sample container at the second activity site for a measurement based on a second incubation time period, the second incubation time period being longer than the first incubation time period. Methods for incubating samples, e.g.

Abstract: The present invention is directed to a method and automated unloading means for unloading a container from an apparatus. The apparatus of the present invention may include a means for automated loading, a means for automated transfer and/or a means for automated unloading of a container (e.g., a specimen container). In one embodiment, the apparatus can be an automated detection apparatus for rapid non-invasive detection of a microbial agent in a test sample. The detection system also including a heated enclosure, a holding means or rack, and/or a detection unit for monitoring and/or interrogating the specimen container to detect whether the container is positive for the presence of a microbial agent. In other embodiment, the automated instrument may include one or more, bar code readers, scanners, cameras, and/or weighing stations to aid in scanning, reading, imaging and weighing of specimen containers within the system.

Abstract: A microwell device is provided. The device includes a plate having a upper surface. The upper surface has first and second recesses formed therein. Each recess has an outer periphery. First and second portions of microwells are formed in upper surface of the plate. The first portion of microwells are spaced about the outer periphery of the first recess and the second portion of microwells spaced about the outer periphery of the first recess. A first barrier is about a first portions of the microwells for fluidicly isolating the first portion of the microwells and a second barrier about a second portions of microwells for fluidicly isolating the second portion of the microwells.

Abstract: A device for assembling aggregations of adherent cells includes a gripper moveable within an assembly vessel that fixes aggregations of adherent cells at a membrane of the gripper and, by movement of the gripper, assembles aggregations of cells on a separate membrane within the vessel, thereby creating a three-dimensional assembly of aggregations of cells that fuse and can be employed in surgical procedures as a unitary tissue of adherent cells. The aggregations of cells, as assembled, can assume three-dimensional configurations distinct from any one of the component aggregations of cells assembled.

Abstract: A sample analyzer is disclosed. The sample analyzer comprises: a reader for reading ID of a sample from a sample container; an aspirator that aspirates a sample in a sample container; an analyzing section that analyzes the aspirated sample; a manual input receiver for receiving a manual input of an ID of a sample; an start instruction receiver for receiving a start instruction to cause the aspirator to aspirate a sample; and a controller. When receiving the start instruction without receiving the manual input, the controller controls the reader to read an ID from a sample container and then controls the aspirator to aspirate the sample from the sample container. When receiving the start instruction after receiving the manual input, the controller controls the aspirator to aspirate a sample without reading of an ID of the sample.

Abstract: An integrated cell culture system may include one or more cell culture vessels, manipulation apparatus, pumping apparatus, cell release apparatus, monitoring apparatus, and a control apparatus. The control apparatus may be used to monitor and control the system to facilitate effective cell culturing. The cell release apparatus may be used to release a plurality of cells adhered to the cell culture surfaces of the cell culture vessels.

Abstract: A thin section fabrication apparatus includes an epi-imaging data acquisition unit that performs imaging by radiating epi-illumination and acquires imaging data, a diffusion imaging data acquisition unit that performs imaging by radiating diffusion illumination and acquires imaging data, an exposed shape extraction unit that extracts an exposed shape of an exposure portion of the biological sample which is exposed to a surface of the embedding block, based on the imaging data acquired by the epi-imaging data acquisition unit, an embedded shape extraction unit that extracts an embedded shape of an embedding portion of the biological sample which is embedded in the embedding block, based on the imaging data acquired by the diffusion imaging data acquisition unit, and a control unit that determines ending of the preliminary cutting by comparing the exposed shape extracted by the exposed shape extraction unit and the embedded shape extracted by the embedded shape extraction unit.

Abstract: To perform accurate measurement in the analysis of chemical components with low concentration, pretreatment such as concentration and purification of samples is essential. For high-throughput of pretreatment of biological samples, various random clinical testing is encountered, where analytes change from sample to sample. The pretreatment apparatus has a separating agent selectively separating a specific component by allowing a sample solution to flow therethrough. A holding section holds a plurality of housing sections, which house the separating agent therein, and has an endless track. A pressurizing section applies pressure to the housing section in a continuous and random-accessible manner; and an extraction solution receiver mechanism selectively receives an extracted solution from the separating agent housed in the housing section. A mass spectrometer is that can be connected to the pretreatment apparatus. Thus, a large number of specimens can be simultaneously processed.