Involving Peroxidase Patents (Class 435/28)
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Publication number: 20130059319Abstract: A method and device are disclosed for continuously detecting, classifying and identifying toxic particles, aerosols and/or vapor in an air sample, in near real time by directing an air sample containing an optional target analyte, in the form of particles, aerosols and/or vapors, enzyme(s), and enzyme substrate(s), to a surface of a collection matrix for forming a biocatalytic reaction product of a plurality of freely mobile optical reporters, and by using a light source with optical reader to interpret the signal from the optical reporter, enabling the detection, classification and identification of toxic particles, aerosols and/or vapor in the air sample.Type: ApplicationFiled: September 2, 2011Publication date: March 7, 2013Inventors: Markus Erbeldinger, David B. Silcott, Jason Aaron Berberich, Keith E. LeJeune
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Patent number: 8383361Abstract: A polymer matrix that may coated on an electrode is created by co-crosslinking (1) an adduct of a polyaniline formed by templated oxidative polymerization on a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme. The polymer matrix may be hydrated, and the absorbed water may make it permeable to, for example, glucose. The polyaniline may be polyaniline itself or a substituted polyaniline; the water-soluble crosslinker may be poly(ethylene glycol)diglycidyl ether, and the redox enzyme may be glucose oxidase. The polymer matrix may be produced by co-crosslinking (1) an adduct of an electrically conductive polymer and a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme in a single step at an about neutral pH, curing by drying. After hydration, the crosslinked polymer matrix may form a 3-dimensional glucose-permeable bioelectrocatalyst, catalyzing the electrooxidation of glucose.Type: GrantFiled: December 14, 2011Date of Patent: February 26, 2013Assignee: Abbott Diabetes Care Inc.Inventors: Adam Heller, Benjamin J. Feldman, Nicolas Mano, Yueh-Lin Loo
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Publication number: 20130045498Abstract: The present invention relates to methods of determining enzyme activity in a fluid, wherein the activity over time provides a viscosity-change in the fluid, by the use of a device (FIG. 1) equipped with at least one pressure sensor (FIG. 1 (4)) to determine the change in the fluid viscosity over time as a measure of the enzyme activity.Type: ApplicationFiled: March 1, 2011Publication date: February 21, 2013Applicant: NOVOZYMES A/SInventors: Gernot J. Abel, Dan Petterson
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Publication number: 20130040289Abstract: A test kit detects the presence of a target analyte in a fluid sample. In particular, the test kit includes reagents capable of detecting the target analyte of interest in breast milk. More particularly, the test kit is capable of detecting the presence of alcohol, caffeine, nicotine, drugs of abuse, therapeutic drugs, triglycerides, lactose, capsaicin, and gluten, for example, in breast milk.Type: ApplicationFiled: October 17, 2012Publication date: February 14, 2013Applicant: Upspring Ltd.Inventor: Upspring Ltd.
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Publication number: 20130023444Abstract: Provided herein are novel reagents and their use in color-producing detection systems for performing diagnostic tests and analytical assays.Type: ApplicationFiled: May 23, 2012Publication date: January 24, 2013Applicant: Diagnostic Innovations, LLCInventor: Victor Kovalenko
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Publication number: 20130023000Abstract: A device is disclosed for conducting a non-invasive analysis of a bodily fluid to determine the presence and level of a certain constituent carried by the bodily fluid. An indicator formulation of the device changes color in response to exposure to the constituent to provide a visible indication of the presence and level of the constituent carried by the bodily fluid. A carrier substrate of the device is constructed of a material having voids providing a high void volume within the substrate. The device is made by applying a chromagen to the carrier substrate to create a chromagen-laden carrier member. Then, a selected reagent having a particular constituent-specific formulation is applied to the chromagen-laden member. The selected reagent then combines with the chromagen, thereby establishing the indicator formulation within the carrier substrate in place for reception of a sample of the bodily fluid.Type: ApplicationFiled: September 7, 2012Publication date: January 24, 2013Applicant: POP TEST LLCInventors: Randice Lisa Altschul, Myron Rapkin, Rebecca O'Brien
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Patent number: 8357494Abstract: The invention concerns anti-clusterin oligoclonal antibodies able to recognize and bind in a selective and specific way antigenic epitopes of clusterin isoforms to be used in tumors diagnosis and in the prediction of their malignancy grade, diagnostic method and related kits.Type: GrantFiled: February 17, 2005Date of Patent: January 22, 2013Assignees: Universita' Delgi Studi “Tor Vergate”, Institute Fisioterapici OspitalieriInventors: Luigi Giusto Spagnoli, Sabina Pucci, Elena Bonanno, Flavia Pichiorri, Gennaro Citro
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Patent number: 8349581Abstract: Methods for characterizing the near term risk of experiencing a major adverse cardiac event in a patient presenting with chest pain are provided. In one embodiment the method comprises determining the level of myeloperoxidase (MPO) activity in a bodily sample obtained from the patient. In another embodiment, the method comprises determining the level of MPO mass in a bodily sample obtained from the patient. In another embodiment, the method comprises determining the level of one or more select MPO-generated oxidation products in a bodily sample obtained from the patient. The select MPO-generated oxidation products are dityrosine, nitrotyrosine, chlorotyrosine, methionine sulphoxide or an MPO-generated lipid peroxidation product.Type: GrantFiled: November 5, 2008Date of Patent: January 8, 2013Assignee: The Cleveland Clinic FoundationInventors: Stanley L. Hazen, Marc S. Penn, Renliang Zhang
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Publication number: 20120328617Abstract: The present invention relates to a method for inhibiting angiogenesis using a peroxiredoxin II (Prx II) inhibitor, and a method for preparing angiogenesis-inhibiting medicines using Prx II inhibitor. According to the present invention, the inhibitor of Prx II gene expression or Prx II protein activity increases oxidative inactivation of VEGF receptor tyrosine kinase (RTK) to reduce VEGF signaling, thereby screening a novel angiogenesis inhibitor. Therefore, the method of the present invention can be used for the prevention or treatment of various diseases, ailments, and conditions related to angiogenesis.Type: ApplicationFiled: June 28, 2012Publication date: December 27, 2012Applicant: Ewha University-Industry Collaboration FoundationInventors: Sang Won Kang, Dong Hoon Kang, Doo Jae Lee
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Publication number: 20120322683Abstract: An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.Type: ApplicationFiled: July 6, 2012Publication date: December 20, 2012Inventors: David J. Liu, Kai Wu, Xing Su
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Patent number: 8329943Abstract: A method of stabilizing a hem protein which is effective against the denaturation and degradation of a hem protein typified by hemoglobin and a storage solution therefor. A method of stabilizing a hem protein and a storage solution therefor characterized in that an iminocarboxylic acid or its salt is made to coexist in a sample containing the hem protein, wherein the above-described iminocarboxylic acid is a compound represented by the following general formula (1) wherein R represents a hydrogen atom or a hydroxyl group; and X's represent each a hydrogen atom, an alkali metal or an ammonium group.Type: GrantFiled: October 6, 2008Date of Patent: December 11, 2012Assignee: Eiken Chemical Co., Ltd.Inventor: Shin Sugo
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Publication number: 20120309036Abstract: The invention relates to an arrangement, comprising a solid carrier and a matrix arranged on the solid carrier, said matrix comprising at least one enzymatically convertible or modifiable molecule and comprising at least one enzyme that can be released by the conversion or modification of the molecule, said enzyme being capable of converting at least one color-changing substrate located in the matrix and/or on the solid carrier.Type: ApplicationFiled: February 9, 2011Publication date: December 6, 2012Inventors: Georg Gübitz, Eva Sigl, Andrea Hasmann, Marc Schröder, Konstantin Schneider, Alexandra Rollett, Franz Kaufmann, Andreas Hafner
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Patent number: 8323914Abstract: The present invention relates to analyte detection test systems, including test systems for the oral detection of analytes in saliva. The present invention also provides compositions and methods for storing multiple assay tests and compositions and methods for measuring the concentration of analytes in a sample.Type: GrantFiled: October 20, 2011Date of Patent: December 4, 2012Assignee: N2itive1 InnovationsInventors: Anthony Toranto, Evan Singer, Brett Miller
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Publication number: 20120301911Abstract: Methods and kits are disclosed for detecting microorganisms that produce glucose oxidase. The method includes providing a culture medium and a hydrogen peroxide indicating reagent comprising a chromogenic substrate that can provide a detectable chromogenic reaction indicating the presence of a microorganism that produces glucose oxidase, and additional methods are disclosed for differentiating microorganisms by the detection of an additional chromogenic reaction.Type: ApplicationFiled: December 21, 2010Publication date: November 29, 2012Inventors: Stephen B. Roscoe, Michael J. Svarovsky, Stephanie J. Moeller
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Patent number: 8293537Abstract: At least one embodiment of a stabilizing system includes a stabilizing agent present in an amount or concentration sufficient to completely or substantially prevent or reduce degradation or inactivation of a glycosylated hemoglobin (HbA1c) in a body fluid and a detection agent capable of detecting the HbA1c.Type: GrantFiled: October 18, 2010Date of Patent: October 23, 2012Inventors: Richard H. Selinfreund, Rakesh Vig, Richard P. Gill
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Patent number: 8287906Abstract: In a process of forming a hydrogel from a mixture comprising hydrogen peroxide (H2O2), horseradish peroxidase (HRP), and a polymer comprising a crosslinkable phenol group, the gelation rate in the solution and the crosslinking density in the hydrogel can be independently adjusted or controlled by selection of the molarity of H2O2 and concentration of HRP in the solution when the molarity of H2O2 is limited to be within a range and the concentration of HRP is limited to be above a threshold. A method for determining the range and threshold is disclosed. The hydrogel may be used to grow cells, in which case, the molarity of H2O2 may be selected to affect the differentiation or growth rate of the cells in the hydrogel.Type: GrantFiled: August 20, 2009Date of Patent: October 16, 2012Assignee: Agency for Science, Technology and ResearchInventors: Motoichi Kurisawa, Li Shan Wang, Joo Eun Chung, Fan Lee
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Publication number: 20120238458Abstract: A method of determining gynaecologic tumor disease in a subject by providing a sample of peripheral blood of the subject, measuring the PPIC expression of cells in the sample, and comparing this to a reference value, the PPIC overexpression being indicative of a gynecologic tumor disease and/or disease progression, including metastatic potential in a gynecologic cancer patient.Type: ApplicationFiled: March 16, 2011Publication date: September 20, 2012Inventors: Dan CACSIRE CASTILLO-TONG, Dietmar PILS, Robert ZEILLINGER, Eva OBERMAYR
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Publication number: 20120231484Abstract: The present invention relates to a method for determining the degree of risk of onset of autism, comprising the step of measuring the triglyceride concentration or the cholesterol concentration in a very low-density lipoprotein fraction of plasma or serum isolated from a subject, or the triglyceride concentration or the cholesterol concentration of plasma or serum. In addition, the present invention provides a kit for determining the degree of risk of onset of autism and a method for screening for a candidate substance for agents for treating autism using a non-human mammal, in which the above described method is utilized.Type: ApplicationFiled: October 14, 2010Publication date: September 13, 2012Inventors: Norio Mori, Kazuhiko Nakamura, Katsuaki Suzuki, Kenji Tsuchiya, Keiko Iwata, Hideo Matsuzaki
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Publication number: 20120202236Abstract: A composite litter material for detecting a disease, such as urinary tract disease, when contacted by feline urine includes an absorptive polymer material forming a solid matrix, exfoliated clay embedded and dispersed within the solid matrix, a chromogenic indicator provided within the solid matrix, and an oxidizing agent distributed and stabilized within the solid matrix to be responsive to peroxidase activity in the urine to activate the chromogenic indicator. A method for detecting or diagnosing a feline disease includes contacting urine with the material and detecting a color change. A method of manufacturing the composite material includes preparing a mixture comprising the absorptive polymer and water and distributing the exfoliated clay, chromogenic indicator and oxidizing agent within the mixture, preferably by extrusion and addition of the components at particular points in processing the polymer mixture.Type: ApplicationFiled: May 21, 2010Publication date: August 9, 2012Applicant: 7905122 CANADA INC.Inventors: Paul Jollez, Isabelle Bolduc, Mohammed Berrada
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Publication number: 20120190054Abstract: Enzymes and/or polypeptides and/or mixtures of interest are evaluated during hydrolysis of cellulosic material by the use of indicator constituents such as fluorescent agents, resulting in efficient high-throughput analysis of enzymes and/or polypeptides. A high-throughput assay for the analysis of inter alia, pretreated corn stover (PCS) hydrolysis is also disclosed.Type: ApplicationFiled: July 13, 2010Publication date: July 26, 2012Applicant: NOVOZYMES A/SInventors: Marco Malten, Keith McFarland
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Publication number: 20120183984Abstract: The invention provides compounds of formula I F-L-Q (I) where F comprises a fluorophore capable of absorbing energy at an excitation wavelength and, in the absence of a quencher, emitting energy at an emission wavelength, which is different than the excitation wavelength; Q comprises a quencher; L comprises a linker moiety having two ends, one end being covalently bound to F and the other end being covalently bound to Q. The compounds are capable of undergoing a reversible reaction (1), provided below: (1) where Q+ is an oxidized form of Q representing the absence of a quencher, Ox comprises an oxidizing agent, which is capable of oxidizing Q to its oxidized form Q, and Red comprises a reducing agent, which is capable of converting Q back to its reduced form Q. The compounds can undergo photo-induced electron transfer when irradiated with energy and when Q exists in its oxidized form, Q+.Type: ApplicationFiled: July 22, 2010Publication date: July 19, 2012Applicant: OPTI MEDICAL SYSTEMSInventors: Huarui He, Chao Lin
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Publication number: 20120183568Abstract: Provided are methods for aiding in diagnosing autoimmune diseases in a mammal, comprising contacting a biological sample that is not a tear sample from the mammal with an antibody that specifically binds to a first polypeptide selected from the group Ctss, Ctsh, Ctsr, Ctsw, Ctsz, Ifng, IL-6ra, IL-10, IL-10ra, IL-15, Tnfa, Apo-F, or Lcn-2 or a second polypeptide selected from the group lactoperoxidase, lactoferrin or lysozyme under conditions favoring the formation of an antibody-polypeptide complex, and determining the amount of complex formed, wherein an increased formation of antibody-first-polypeptide complex or a decreased formation of antibody-second-polypeptide complex as compared to a suitable control, indicates a likely positive diagnosis of an autoimmune disease for the mammal, thereby aiding in the diagnosis. Methods of treating the autoimmune diseases are also provided.Type: ApplicationFiled: December 13, 2011Publication date: July 19, 2012Inventor: Sarah Hamm-Alvarez
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Patent number: 8221967Abstract: A determination method quantifies a specific protein or peptide contained in a trace amount with high accuracy and in a simple manner without the need of using any expensive reagent. A protein or peptide of interest can be quantified by LC/MS/MS by using, as an internal standard, a protein or peptide including an amino acid sequence having the reshuffling the binding order of amino acid residues in the amino acid sequence for the protein or peptide of interest.Type: GrantFiled: December 27, 2007Date of Patent: July 17, 2012Assignee: Megmilk Snow Brand Co., Ltd.Inventors: Yoshikazu Morita, Aiko Ono, Atsushi Serizawa, Hiroshi Kawakami
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Publication number: 20120171693Abstract: The disclosure provides methods for identifying and producing stabilized chimeric proteins.Type: ApplicationFiled: January 5, 2008Publication date: July 5, 2012Applicant: THE CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Frances H. Arnold, Yougen Li
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Publication number: 20120164674Abstract: Herein are disclosed compositions, washes, rinses, devices, systems and methods for biomarker stabilization, in part in bodily fluids, as well as methods and formulations for improving the sampling and testing of fluids for biomarkers.Type: ApplicationFiled: October 27, 2011Publication date: June 28, 2012Inventors: Richard H. Selinfreund, Rakesh Vig, Richard P. Gill
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Patent number: 8192942Abstract: The present invention relates to a method for separating a material that has affinity to an antibody by using a protein-antibody conjugate with modified partition characteristics, more precisely a method for affinity separation to separate a material specifically binds to an antibody, in which an antibody is conjugated to a protein to modify partition characteristics of the protein-antibody conjugate. The method of the present invention can be effectively and widely used as a safe and efficient separation method for biomolecules since it takes advantages of safe aqueous two-phase extraction system and high selective molecular specific conjugation.Type: GrantFiled: January 21, 2008Date of Patent: June 5, 2012Assignee: INHA-Industry Partnership InstituteInventors: Yoon-Mo Koo, Woo-Jin Chang, Hye-Mee Park
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Patent number: 8192948Abstract: A kit for enhanced detection of analytes that produce peroxide or peroxidase activity.Type: GrantFiled: April 17, 2009Date of Patent: June 5, 2012Assignee: Pierce Biotechnology, Inc.Inventor: Kelli D. Feather-Henigan
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Publication number: 20120129187Abstract: The present invention relates to a method for the diagnosis or prognosis of a disease or clinical condition in a subject comprising the steps of: (i) providing a sample of bodily fluid of a subject, (ii) determining the level of peroxiredoxin 4 (PRX4) or a fragment thereof having at least 20 amino acids residues in length in said sample, and (iii) correlating the level of PRX4 or a fragment thereof with a disease or clinical condition.Type: ApplicationFiled: June 15, 2010Publication date: May 24, 2012Applicant: B.R.A.H.M.S. GMBHInventors: Joachim Struck, Janina Schulte
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Publication number: 20120100563Abstract: A device is disclosed for conducting a non-invasive analysis of a bodily fluid to determine the presence and level of a certain constituent carried by the bodily fluid. An indicator formulation of the device changes color in response to exposure to the constituent to provide a visible indication of the presence and level of the constituent carried by the bodily fluid. A carrier substrate of the device is constructed of a material having voids providing a high void volume within the substrate. The device is made by applying a chromagen to the carrier substrate to create a chromagen-laden carrier member. Then, a selected reagent having a particular constituent-specific formulation is applied to the chromagen-laden member. The selected reagent then combines with the chromagen, thereby establishing the indicator formulation within the carrier substrate in place for reception of a sample of the bodily fluid.Type: ApplicationFiled: October 21, 2011Publication date: April 26, 2012Applicant: POP TEST LLCInventors: Randice Lisa Altschul, Myron Rapkin, Rebecca O'Brien
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Publication number: 20120076815Abstract: This invention provides identification and characterization of racemases and definition of protein signatures of these racemases. This invention also provides identification of nucleic acid molecules encoding a peptide consisting of a motif characteristic of the protein signatures, and to the peptides consisting of these motifs. Antibodies specific for the peptides and to immune complexes of these antibodies with the peptides are also provided. Further, the invention relates to methods and kits for detecting racemases using the nucleic acid molecules of the invention, as well as the peptides consisting of the motifs and antibodies to these peptides.Type: ApplicationFiled: September 30, 2010Publication date: March 29, 2012Inventors: Paola Minoprio, Nathalie Chamond, Wim M. Degrave, Armand Berneman
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Publication number: 20120077211Abstract: Compositions, assays, methods, and kits are disclosed for use in applications that utilize oxidation of a chromogenic electron donor such as diaminobenzidine (DAB) to generate a signal. Applications include, but are not limited to, immunohistochemistry, chromogenic in situ hybridization, Western blots, Northern blots, Southern blots, ELISA assays, and microarray detection. The compositions, assays, methods, and kits disclosed herein make use of a novel, stabilized formulation of DAB and a novel, stabilized formulation of hydrogen peroxide.Type: ApplicationFiled: December 15, 2009Publication date: March 29, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Lawrence Greenfield, Shawn Starkenburg, Matthew Shallice, Julie Nyhus, Louis Leong
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Publication number: 20120071339Abstract: The invention relates to a method of diagnosing or monitoring multiple sclerosis.Type: ApplicationFiled: February 26, 2010Publication date: March 22, 2012Applicant: PSYNOVA NEUROTECH LTD.Inventors: Sabine Bahn, Emanuel Schwarz
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Publication number: 20120064559Abstract: According to one embodiment, a test element includes a base, a pair of optical element units, an optical waveguide unit, a detection unit and a holding unit. The base has transparency. The pair of optical element units are arranged away from each other on a major surface of the base. The optical waveguide unit is provided on the major surface of the base. The detection unit is provided on a major surface of the optical waveguide unit of between the optical element units. The major surface of the optical waveguide unit is an opposite side which touches the base. The holding unit is in a frame shape, and one end of the holding unit being is provided to protrude from a major surface of the detection unit. The detection unit includes a color former and a film-formed body holding the color former.Type: ApplicationFiled: August 11, 2011Publication date: March 15, 2012Applicant: Kabushiki Kaisha ToshibaInventors: Ichiro TONO, Takaaki Wada, Kayoko Oomiya, Shingo Kasai, Masaaki Hirakawa
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Patent number: 8133718Abstract: An analytical strip and a detecting method using the analytical strip are provided. The analytical strip includes a substrate having a channel thereon. The channel has a first region, a second region and a third region, which are arranged successively. A first antibody is localized in the first region. A saccharide and a peroxidase are localized in the first or second region. A second antibody for recognizing a different epitope of an identical antigen with the first antibody is immobilized in the second region. A substrate reagent including a saccharide oxidase is localized in the third region.Type: GrantFiled: April 29, 2009Date of Patent: March 13, 2012Assignee: Actherm IncInventors: Yi-Jen Wu, Chih-Wei Hsieh, Wen-Pin Hsieh
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Patent number: 8129136Abstract: The present invention relates to a kit for performing an assay. The kit comprises luminol, an oxidant, an electron mediator and a hypernucleophilic acylation catalyst.Type: GrantFiled: August 20, 2010Date of Patent: March 6, 2012Assignee: Cyanagen SrlInventor: Leopoldo Della Ciana
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Publication number: 20120034639Abstract: The present invention relates to analyte detection test systems, including test systems for the oral detection of analytes in saliva. The present invention also provides compositions and methods for storing multiple assay tests and compositions and methods for measuring the concentration of analytes in a sample.Type: ApplicationFiled: October 20, 2011Publication date: February 9, 2012Applicant: N2ITIVE1 INNOVATIONSInventors: Anthony Toranto, Evan Singer, Brett Miller
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Publication number: 20120034637Abstract: The invention relates to a method and a kit for the detection of Trichomonas infection by detecting N-acetyl-?-D-hexosaminidase activity. The invention also relates to a method and a kit for selective detection of presence of Trichomonas or Candida infection.Type: ApplicationFiled: January 27, 2010Publication date: February 9, 2012Inventors: Zeyu Wang, Hongyun Yang, Guangyu Fu, Xuewei Wu, Chaojie Feng, Yang Yang, Jingjing Yang, Yuanyuan Xie, Yehuan Zheng, Guilin Li, Fan Yang
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Publication number: 20120034632Abstract: Methods and kits for performing a two-phase optical assay for one or more than one analyte without intrinsic optical contrast in a sample are disclosed. The method requires use of a functionalized microparticle immobilized with two or more than functional components and an additional set of one or more than one functional component. The assay can be performed in one single container and does not need a wash step.Type: ApplicationFiled: August 5, 2011Publication date: February 9, 2012Inventors: Paul A. SAUNDERS, Alex M. Saunders
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Publication number: 20120028250Abstract: Compositions, devices, systems and methods for reducing and/or preventing photodamage of one or more reactants in illuminated analytical reactions by one or more of incorporating photodamage mitigating agents within the reaction mixture and/or interrogating different observation regions of the reaction mixture for a period that is less than a photodamage threshold period.Type: ApplicationFiled: July 26, 2011Publication date: February 2, 2012Applicant: Pacific Biosciences of California, Inc.Inventors: John Eid, Devon Murphy, Geoffrey Otto, Stephen Turner
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Patent number: 8101374Abstract: It is an object of the present invention to provide a means for measuring a concentration of various components rapidly with high accuracy and high sensitivity. The present invention is to provide an oxidative chromogenic compound or salt thereof represented by the following chemical formula (1): wherein R1 and R2 are each independently a linear or branched alkyl group having 1 to 5 carbon atoms; R3, R4, R5, R6 and R7 are each independently a hydrogen atom, a carboxyl group or a sulfonic acid group, or salt thereof, provided that at least one of R3 to R7 is a carboxyl group or a sulfonic acid group, or salt thereof, and the others are hydrogen atoms, and the production method thereof, and the reagent composition and test instrument using the same.Type: GrantFiled: March 6, 2008Date of Patent: January 24, 2012Assignee: Terumo Kabushiki KaishaInventors: Tetsuro Kawanishi, Yuzuru Saito, Takako Ariga
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Publication number: 20120015953Abstract: The present invention relates to compositions comprising an antagonist of the histamine H1 receptor and a compatible antagonist of the histamine H4 receptor. The antagonist compounds are selected to prevent the H4 receptor antagonist from interfering with the H1 receptor antagonist's suppression of acute phase allergic responses in a patient. The present invention also relates to compositions comprising a mast cell stabilizer and a compatible antagonist of the histamine H4 receptor.Type: ApplicationFiled: July 14, 2011Publication date: January 19, 2012Inventors: Clay Beauregard, Peter G. Klimko, John M. Yanni, Daniel A. Gamache
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Publication number: 20120009174Abstract: This invention relates, e.g., to a method for determining if a subject has myocardial ischemia, comprising (a) providing a blood sample obtained from a subject suspected of having myocardial ischemia; (b) determining in the sample the amount of one or more of the following proteins: (i) Lumican and/or (ii) Extracellular matrix protein 1 and/or (iii) Carboxypeptidase N; and (c) comparing the amount(s) of the protein(s) to a baseline value that is indicative of the amount of the protein in a subject that does not have myocardial ischemia, wherein a statistically significantly increased amount of the protein(s) compared to the baseline value is indicative of myocardial ischemia. Other proteins indicative of myocardial ischemia are also described, as are methods for treating a subject based on a diagnostic procedure of the invention, and kits for carrying out a method of the invention.Type: ApplicationFiled: May 26, 2009Publication date: January 12, 2012Applicant: THE JOHNS HOPKINS UNIVERSITYInventors: Jennifer Van Eyk, Simon Sheng, Qin Fu
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Publication number: 20120010095Abstract: The invention relates to diagnostic and prognostic assays and kits for pulmonary hypertension (PH) and types thereof. The invention includes detecting the expression level of markers associated with pulmonary hypertension for diagnosis, prognosis, or treatment of pulmonary hypertension.Type: ApplicationFiled: June 1, 2011Publication date: January 12, 2012Inventors: Peter L. Jones, Darren Taichman
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Publication number: 20120003677Abstract: Provided are methods of determining whether a cell in a tissue site is viable or nonviable. Also provided are methods of debriding tissue from a tissue site. Further provided are kits comprising a compound that distinguishes between viable and nonviable cells and instructions for using the compound on a tissue site. Additionally, the use of a compound that distinguishes between viable and nonviable cells is provided, where the use is to determine whether a cell in a tissue site is viable or nonviable. Also provided is a use of a compound that distinguishes between viable and nonviable cells, where the use is for the manufacture of the above-described kit.Type: ApplicationFiled: September 13, 2011Publication date: January 5, 2012Inventors: Amy McNulty, Kristine Kieswetter, Todd Fruchterman
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Publication number: 20110321197Abstract: A method for producing a plant with increased yield as compared to a corresponding wild type plant whereby the method comprises at least the following step: increasing or generating in a plant or a part thereof one or more activities selected from the group consisting of 17.6 kDa class I heat shock protein, 26.Type: ApplicationFiled: October 2, 2009Publication date: December 29, 2011Applicant: BASF Plant Science GmbHInventors: Hardy Schön, Oliver Thimm, Gerhard Ritte, Oliver Bläsing, Koen Bruynseels, Yves Hatzfeld, Valerie Frankard, Ana Isabel Sanz Molinero, Christophe Reuzeau, Steven Vandenabeele
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Publication number: 20110311505Abstract: This invention is related to preparation of photosensitive ruthenium based aminoacid monomers and oligomers, aminoacid monomer-protein cross-linking using photo sensitat ion and conjugation on micro and nano-structures by ruthenium-chelate based monomers. Its vast range biotechnolgy applications of multifunctional, biocompatible, stabilE and specific micro and nanobio-conjugates, which will stand-alone or simultaneously enable (i) both purification and determination, (ii) both targeting and imaging and theranostics and (iii) catalysis and determination. The construction and method of preparation is applicable to silica materials, superparamagnetic particles, QDs, CNTs, Ag/Au nanoparticles and Au surfaces and polymeric materials. The photosensitive aminoacid monomer linkers can react via chemically and biocompatible to a lot of different micro and nano-surface and then to the protein when they act as a single-step cross-linking reaction using irradiation.Type: ApplicationFiled: December 11, 2009Publication date: December 22, 2011Inventors: Arzu Ersoz, Deniz Hur, Filiz Yilmaz, Adil Denizli, Ayca Atilir Ozcan, Sibel Emir Diltemiz, Suzan Yazar, Ozlem Bicen, Sibel Buyuktiryaki, Rustem Kecili, Guner Saka, Tugba Findik
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Patent number: 8080385Abstract: A polymer matrix that may coated on an electrode is created by co-crosslinking (1) an adduct of a polyaniline formed by templated oxidative polymerization on a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme. The polymer matrix may be hydrated, and the absorbed water may make it permeable to, for example, glucose. The polyaniline may be polyaniline itself or a substituted polyaniline; the water-soluble crosslinker may be poly(ethylene glycol) diglycidyl ether, and the redox enzyme may be glucose oxidase. The polymer matrix may be produced by co-crosslinking (1) an adduct of an electrically conductive polymer and a polymer acid; (2) a water-soluble crosslinker; and (3) a redox enzyme in a single step at an about neutral pH, curing by drying. After hydration, the crosslinked polymer matrix may form a 3-dimensional glucose-permeable bioelectrocatalyst, catalyzing the electrooxidation of glucose.Type: GrantFiled: May 2, 2008Date of Patent: December 20, 2011Assignee: Abbott Diabetes Care Inc.Inventors: Adam Heller, Benjamin J. Feldman, Nicolas Mano, Yueh-Lin Loo
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Patent number: 8080423Abstract: Stabilizers for colorants used for detection of enzymatic reactions, having a cyclodextrin and a catalase; reagents including stabilizers; and methods of use of such stabilizers and reagents.Type: GrantFiled: August 4, 2005Date of Patent: December 20, 2011Assignee: Asahi Kasei Pharma CorporationInventors: Takuji Kouzuma, Yoko Nagai, Shigeyuki Imamura
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Patent number: 8067188Abstract: The present invention relates to analyte detection test systems, including test systems for the oral detection of analytes in saliva. The present invention also provides compositions and methods for storing multiple assay tests and compositions and methods for measuring the concentration of analytes in a sample.Type: GrantFiled: April 16, 2010Date of Patent: November 29, 2011Assignee: N2ITIVE1 InnovationsInventors: Anthony Toranto, Evan Singer, Brett Miller
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Publication number: 20110287468Abstract: The present invention provides a two-step assay for measuring myeloperoxidase (MPO) activity in a blood sample. The first step utilizes a chromogenic substrate to measure first peroxidase activity including MPO activity in the sample, whereas the second step measures non-MPO peroxidase activity in the presence of the same chromogenic substrate and a specific MPO inhibitor. Specific MPO peroxidase activity is then determined by comparing the non-MPO peroxidase activity and the total peroxidase activity. The MPO peroxidase activity obtained in this fashion may be proportional, and preferably directly proportional, to the mass of MPO in the sample. Kits for assaying MPO peroxidase activity based on the same principle are also provided.Type: ApplicationFiled: April 19, 2011Publication date: November 24, 2011Applicant: General AtomicsInventors: Chong-Sheng YUAN, Xiaomin GONG