With Significant Amplification Step (e.g., Polymerase Chain Reaction (pcr), Etc.) Patents (Class 435/6.12)
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Patent number: 11332789Abstract: The invention provides compositions and methods for the diagnosis, treatment, assessment, and characterization of hyperlipidemia-related diseases and disorders, including atherosclerosis, non-alcoholic fatty liver disease, obesity and diabetes mellitus in a subject in need thereof, based on the expression level of at least one miRNA that is associated with these diseases and disorders.Type: GrantFiled: May 21, 2019Date of Patent: May 17, 2022Assignee: Temple University-Of The Commonwealth System of Higher EducationInventor: Xiao-Feng Yang
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Patent number: 11326206Abstract: The present disclosure provides compositions, methods and systems for quantifying target sequences and identifying target sequence variants.Type: GrantFiled: April 6, 2017Date of Patent: May 10, 2022Assignee: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.Inventors: Yi Zhang, Steven Brentano, Eugene Tu, Kandaswamy Vijayan
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Patent number: 11326205Abstract: The invention relates to a method for the duplication of nucleic acids by means of a polymerase chain reaction, in the case of which a cycle consisting of the steps of denaturing, annealing and elongation is repeatedly performed. In one embodiment, in at least one passage of the cycle, the quotient of the duration of effect tA and the reaction volume Vr irradiated by the energy source is less than 1 seconds per microliter. In another embodiment, in at least one passage of the cycle, the ratio of the duration of effect (tA) and the duration of the PCR cycle (tc) is less than 20%. In certain embodiments, the yield (g) of nucleic acids at the end of at least one of the passages of the cycle is less than 80% of the nucleic acids present at the start of the passage.Type: GrantFiled: December 6, 2019Date of Patent: May 10, 2022Assignee: GNA Biosolutions GmbHInventors: Federico Buersgens, Joachim Stehr, Lars Ullerich
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Patent number: 11326148Abstract: The presently disclosed subject matter provides for in vitro methods of inducing differentiation of human stem cells into neural crest, cranial placode or non-neuro ectoderm precursors, and cells generated by such methods. The presently disclosed subject matter also provides for uses of such cells for treating neurodegenerative and pituitary disorders.Type: GrantFiled: August 3, 2018Date of Patent: May 10, 2022Assignee: MEMORIAL SLOAN-KETTERING CANCER CENTERInventors: Lorenz Studer, Bastian Zimmer, Jason Tchieu
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Patent number: 11319578Abstract: The present invention provides assays systems and methods for detection of chromosomal abnormalities and status of single loci associated with monogenic or polygenic traits in a sample containing nucleic acids from a maternal and a fetal source.Type: GrantFiled: December 21, 2017Date of Patent: May 3, 2022Assignee: Roche Molecular Systems, Inc.Inventors: Andrew Sparks, Arnold Oliphant, Jacob Zahn, Ken Song, John Stuelpnagel
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Patent number: 11319534Abstract: Embodiments of the present invention relate to sequencing nucleic acids. In particular, embodiments of the methods and compositions provided herein relate to preparing nucleic acid templates and obtaining sequence data therefrom.Type: GrantFiled: January 6, 2020Date of Patent: May 3, 2022Assignee: ILLUMINA, INC.Inventors: Frank J. Steemers, Sasan Amini, Kevin L. Gunderson, Natasha Pignatelli, Igor Goryshin
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Patent number: 11319590Abstract: Disclosed are methods for sequencing immune cell receptor repertoires from immune cell populations, the methods comprising isolating RNA from immune cells, generating cDNA from the RNA, ligating adapter sequences to the cDNA, and sequencing the cDNA. Also provided are kits containing primer mixtures for the sequencing of immune cell receptor repertoires.Type: GrantFiled: January 30, 2018Date of Patent: May 3, 2022Assignee: Ludwig Institute for Cancer Research Ltd.Inventor: Raphael Genolet
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Patent number: 11322228Abstract: A method of designing a D-polypeptide that binds with an L-target protein can include: identifying a polypeptide target having L-chirality; determining hotspot amino acids of a polypeptide ligand having L-chirality that have binding interactions with the L-target protein; determining transformations of side chains of the hotspot amino acids that retain the binding interactions with the target; generating inversed hotspot amino acids with chirality opposite to the one of the target; identifying a polypeptide having inverse chirality from the target protein, on which a combination of inversed hotspot amino-acid can be grafted without significantly changing their interactions with the target. The designed ligands can be processed and converted to D-ligands that bind with the L-target protein.Type: GrantFiled: October 27, 2016Date of Patent: May 3, 2022Assignee: Janssen Vaccines & Prevention B.V.Inventors: Jaroslaw Juraszek, Davide Branduardi, Ronald Vogels, Robert Heinz Edward Friesen
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Patent number: 11319582Abstract: Provided herein are methods for isolating nucleic acids from intact cells in a sample of intact cells, contamination dead cells, cell debris, and biofilm using two separation steps, either by centrifugation or filtration, performed in sequentially. Also provided is a method for isolating nucleic acids from intact cells using a first separation step followed by treatment with a nuclease and then a second separating step. Provided herein is a related method for isolating DNA from intact cells using a nuclease that produces DNA cuts on double stranded DNA, followed by a second separating step.Type: GrantFiled: March 12, 2020Date of Patent: May 3, 2022Assignee: PathogenDx, Inc.Inventors: Michael Edward Hogan, Benjamin Alan Katchman, Candy Mavis Rivas, Yasmine Eve Baca
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Patent number: 11322224Abstract: The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a mixed sample of DNA comprising DNA from both the mother of the fetus and from the fetus, and optionally from genotypic data from the mother and father. The ploidy state is determined by using a joint distribution model to create a plurality of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR.Type: GrantFiled: November 4, 2014Date of Patent: May 3, 2022Assignee: Natera, Inc.Inventors: Matthew Rabinowitz, George Gemelos, Milena Banjevic, Allison Ryan, Zachary Demko, Matthew Hill, Bernhard Zimmermann, Johan Baner
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Patent number: 11315023Abstract: Methods and systems for storing digital data into peptide sequences and retrieving digital data from peptide sequences are disclosed. The method for storing digital data into peptide sequences may include: encoding the digital data into a digital code; translating the digital code into the peptide sequences; and synthesizing the translated peptide sequences. The method for retrieving digital data from peptide sequences may include: sequencing and determining an order of the peptide sequences; converting the peptide sequences with the determined order into a digital code; and decoding the digital data from the digital code. Codes with error-correction capability are developed for encoding digital data into peptide sequences, and a computational method implemented in a software is developed for sequencing the digital data bearing peptides.Type: GrantFiled: December 19, 2018Date of Patent: April 26, 2022Assignee: THE HONG KONG POLYTECHNIC UNIVERSITYInventors: Zhongping Yao, Cheuk Chi Albert Ng, Chung Ming Lau, Wai Man Tam
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Patent number: 11312755Abstract: The invention relates generally to the field of nanopores and the use thereof in various applications, such as analysis of biopolymers and macromolecules, typically by making electrical measurements during translocation through a nanopores. Provided is a system comprising a funnel-shaped proteinaceous nanopore comprising an a-helical pore-forming toxin that is a member from the actinoporin protein family, more in particular Fragaceatoxin C (FraC), a mutant FraC, a FraC paralog, or a FraC homolog.Type: GrantFiled: May 24, 2017Date of Patent: April 26, 2022Assignee: Rijksuniversiteit GroningenInventors: Giovanni Maglia, Carsten Wloka, Natalie Lisa Mutter, Misha Soskine, Gang Huang
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Patent number: 11313002Abstract: Disclosed are methods utilizing specific amplification of Candida sp. target nucleic acid for detecting the presence or absence of Candida sp. in a sample. Also disclosed are corresponding oligomers, including amplification oligomers, capture probes and detection probes, and combinations thereof, as well as corresponding reaction mixtures and kits.Type: GrantFiled: March 19, 2021Date of Patent: April 26, 2022Assignee: GEN-PROBE INCORPORATEDInventors: Angela S. Hudson, Damon K. Getman, Alice Jiang, Barbara L. Eaton
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Patent number: 11312997Abstract: Technology provided herein relates in part to methods, processes and apparatuses for non-invasive assessment of genetic variations.Type: GrantFiled: May 27, 2020Date of Patent: April 26, 2022Assignee: Sequenom, Inc.Inventors: Charles R. Cantor, Grace DeSantis, Reinhold Mueller, Mathias Ehrich
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Patent number: 11306349Abstract: The present invention relates to the detection of a target nucleic acid sequence by a PTOCE (PTO Cleavage and Extension) assay. The present invention detects a target nucleic acid sequence in which the PTO (Probing and Tagging Oligonucleotide) hybridized with the target nucleic acid sequence is cleaved to release a fragment and the fragment is hybridized with the CTO (Capturing and Templating Oligonucleotide) to form an extended duplex, followed by detecting the presence of the extended duplex. The extended duplex provides signals (generation, increase, extinguishment or decrease of signals) from labels indicating the presence of the extended duplex and has adjustable Tm value, which are well adoptable for detection of the presence of the target nucleic acid sequence.Type: GrantFiled: December 2, 2019Date of Patent: April 19, 2022Assignee: SEEGENE, INC.Inventors: Jong Yoon Chun, Young Jo Lee
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Patent number: 11304638Abstract: Methods and devices are provided for sample collection. In one example, a device is provided comprising at least one capillary tube or collection channel directed to a sample vessel, wherein in a one-step removal step of detaching the sample vessel from the collection channel, a vacuum force is created within the sample vessel, due in part of the pulling of the sealed vessel away from the device, wherein this vacuum force draw out residual sample that may still be resident in the collection channel.Type: GrantFiled: May 8, 2020Date of Patent: April 19, 2022Assignee: Labrador Diagnostics LLCInventor: Zachary Morrison
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Patent number: 11306350Abstract: Specific, accurate, and cost effective primers for performing digital PCR, compositions and kits containing the primer and methods for using and making the same are useful for detecting nucleic acid mutations. A primer useful as a first forward primer in performing digital PCR to detect a target nucleic acid in a sample, includes: a detection portion located upstream to a target sequence binding portion, and including a second forward primer binding portion having a sequence substantially complementary to a second forward primer, and a probe binding portion downstream to the second forward primer binding portion having a sequence substantially complementary to a probe; the target sequence binding portion includes a mismatch portion having a sequence not complementary to the target nucleic acid, and an amplification determinant portion downstream to the mismatch portion having a sequence complementary to a gene allele or a variant thereof encoded by the target nucleic acid.Type: GrantFiled: April 15, 2019Date of Patent: April 19, 2022Assignee: Maccura Biotechnology Co., Ltd.Inventors: Yuhang Zhao, Shufang Wang, Zhiqi Ge
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Patent number: 11309058Abstract: Techniques are described for determining the strain on a cell wall using two models: 1) a short timescale model, describing the relationship between physical properties assumed to be fixed and 2) a long timescale model, describing the dynamic chemical composition of a cell wall. Short term modeling of the physical properties in a cell wall is used to properly understand how physical factors such as osmotic pressure affects the strain on the cell wall, which is in turn used to identify conditions under which a cell wall will cease to function properly or lyse entirely. Although temporally the physical properties which cause cell walls to underperform/lyse can be evaluated under a short time frame, the chemical properties that lead to the physical properties which cause that behavior themselves change over much longer timescales, in a relative sense.Type: GrantFiled: March 30, 2018Date of Patent: April 19, 2022Assignee: X Development LLCInventor: Nicholas Casavant
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Patent number: 11306359Abstract: A system and method for determining the genetic data for one or a small set of cells, or from fragmentary DNA, where a limited quantity of genetic data is available, are disclosed. Genetic data for the target individual is acquired and amplified using known methods, and poorly measured base pairs, missing alleles and missing regions are reconstructed using expected similarities between the target genome and the genome of genetically related subjects. In accordance with one embodiment of the invention, incomplete genetic data is acquired from embryonic cells, fetal cells, or cell-free fetal DNA isolated from the mother's blood, and the incomplete genetic data is reconstructed using the more complete genetic data from a larger sample diploid cells from one or both parents, with or without genetic data from haploid cells from one or both parents, and/or genetic data taken from other related individuals.Type: GrantFiled: February 28, 2019Date of Patent: April 19, 2022Assignee: Natera, Inc.Inventors: Matthew Rabinowitz, Milena Banjevic, Zachary Demko, David Johnson
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Patent number: 11299766Abstract: Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.Type: GrantFiled: May 29, 2020Date of Patent: April 12, 2022Assignee: Exact Sciences CorporationInventors: Graham P. Lidgard, Hatim Allawi
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Patent number: 11299730Abstract: Contiguity information is important to achieving high-quality de novo assembly of mammalian genomes and the haplotype-resolved resequencing of human genomes. The methods described herein pursue cost-effective, massively parallel capture of contiguity information at different scales.Type: GrantFiled: October 28, 2019Date of Patent: April 12, 2022Assignee: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATIONInventors: Jay Ashok Shendure, Jerrod Joseph Schwartz, Andrew Colin Adey, Cho li Lee, Joseph Brian Hiatt, Jacob Otto Kitzman, Akash Kumar
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Patent number: 11299726Abstract: Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50° C. to about 100° C.; and recovering the nucleic acid from said lysis solution.Type: GrantFiled: January 23, 2019Date of Patent: April 12, 2022Assignee: CEPHEIDInventors: Edwin Wei-Lung Lai, Reuel Van Atta, Kenneth E. Ho
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Patent number: 11299764Abstract: A screening method for the identification of a characteristic of a target nucleic acid in a whole blood sample, including positioning a composition comprising whole blood and at least one preservative agent within a centrifuge, centrifugating the composition to isolate a plasma that includes at least one target nucleic acid for further analysis and analyzing the at least one target nucleic acid to identify a characteristic about the at least one target nucleic acid, and a composition including the plasma, the preservative agent, and any other ingredient, which is produced by the method.Type: GrantFiled: December 27, 2018Date of Patent: April 12, 2022Assignee: STRECK, INC.Inventors: Bradford A. Hunsley, Jianbing Qin
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Patent number: 11299779Abstract: A method of identifying a polynucleic acid (PNA) is presented, including the steps of providing a PNA; modifying one or more nucleobases of the PNA by addition or removal of a hydrogen bonding partner, thereby altering the base pairing capacity of the one or more nucleobases; base pairing a complementary nucleic acid to the PNA, including base pairing to at least one modified nucleobase; identifying the sequence of the complementary nucleic acid at least at the position that is complementary to at least one modified nucleobase.Type: GrantFiled: April 13, 2018Date of Patent: April 12, 2022Assignee: IMBA—INSIIIUT FÜR MOLEKULARE BIOTECHNOLOGIE GMBHInventors: Stefan L. Ameres, Brian Reichholf, Veronika A. Herzog, Johannes Zuber, Matthias Muhar
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Patent number: 11299772Abstract: The present invention provides assay systems and methods for detection of copy number variation at one or more loci and polymorphism detection at one or more loci in a mixed sample from an individual.Type: GrantFiled: May 23, 2019Date of Patent: April 12, 2022Assignee: Ariosa Diagnostics, Inc.Inventors: Andrew Sparks, Craig Struble, Eric Wang, Arnold Oliphant
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Patent number: 11293056Abstract: The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.Type: GrantFiled: November 7, 2018Date of Patent: April 5, 2022Assignee: Life Technologies CorporationInventors: Abraham Rosenbaum, Collyn Seeger, Jeremy Gray, Hua Yu
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Patent number: 11291990Abstract: The invention discloses a quasi-volumetric sensing system and method. Plural short-range order (SRO) units are configured on the carrier of a quasi-volumetric device, and arranged as an array, i.e. a long-range order (LRO) unit. Protrusions, configured on the SRO units, can modify the wettability of the carrier to control the liquid volume retained thereon so that the precise volume of the liquid sample or droplets are calculated. Based on the applied force on the LRO unit and the gradient of hydrophilicity-hydrophobicity on the surface, the redundant volume of the liquid sample is removed. Macromolecules, e.g. antibodies, complements, receptor proteins, aptamers, oligosaccharides or oligonucleotides, configured on the protrusions are coupled to specific molecules in the liquid sample or droplets so as to determine characteristics of the specific molecules. Therefore, the open chip device of the invention can be used to achieve the quasi-volumetric measurement and the analysis of specific molecules.Type: GrantFiled: December 2, 2019Date of Patent: April 5, 2022Assignee: Tai-Saw Technology Co., Ltd.Inventors: Szu-Heng Liu, Yi-Qi Huang, Yu-Tung Huang
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Patent number: 11286493Abstract: The present disclosure relates to the use of recombinant proteins for inducing epigenetic modifications at specific loci, as well as to methods of using these recombinant proteins for reducing the expression of genes in plants.Type: GrantFiled: May 26, 2017Date of Patent: March 29, 2022Assignee: The Regents of the University of CaliforniaInventors: Steve E. Jacobsen, Javier Gallego-Bartolomé, Ashot Papikian
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Patent number: 11289318Abstract: The invention provides a mass spectrometry analysis method and a mass spectrometry system. During implementation of the mass spectrometry analysis method, intensity data of the daughter ions, a first parameter of the daughter ions associated with the first physicochemical property, and a second parameter of the daughter ions associated with the second physicochemical property are all recorded to form a spectrogram data set. In a deconvolution step, the spectrogram data set is deconvoluted to categorize the daughter ions from the same parent ion according to two-dimensional features including the first parameter and the second parameter. In the above manner, the mass spectrometry analysis method and the mass spectrometry system provided by the invention can detect ions that partially overlap spectral peaks of other ions significantly, thereby improving the qualitative and quantitative ability of data analysis for data independent acquisition.Type: GrantFiled: March 25, 2021Date of Patent: March 29, 2022Assignee: SHIMADZU CORPORATIONInventors: Xinfeng Zhou, Ran Qiu, Wenjian Sun
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Patent number: 11287631Abstract: A microscope stage is movably mounted to a base and is movable relative to the base by two separate drive units on separate rail systems situated at an angle relative to one another. The drive units are mechanically isolated from one another and one of the drive units uses a belt drive.Type: GrantFiled: June 22, 2018Date of Patent: March 29, 2022Inventors: David Istvan Szocs, Janos Tibor Devenyi, Viktor Sebestyen Varga, Bela Molnar
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Patent number: 11286522Abstract: The present disclosure relates to systems and methods for high efficiency electronic sequencing of nucleic acids and molecular detection.Type: GrantFiled: August 20, 2018Date of Patent: March 29, 2022Assignee: GENAPSYS, INC.Inventor: Hesaam Esfandyarpour
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Patent number: 11279939Abstract: The invention provides compositions and methods for regulating intracellular osmolarity in cells, e.g., in cultured cells, including in cultured cells in bioreactors. The invention provides nucleic acids comprising at least one osmo-responsive transcriptional regulatory element (OR-TRE), and cells, vectors, products of manufacture, artificial organs or implants and the like containing an osmo-responsive transcriptional regulatory element (OR-TRE).Type: GrantFiled: September 26, 2018Date of Patent: March 22, 2022Assignee: GENENTECH, INC.Inventors: Laetitia Malphettes, Andrew Snowden, Inn H. Yuk
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Patent number: 11274340Abstract: Methods, compositions and kits for capturing, detecting and quantifying mature small RNAs are provided herein. Embodiments of the methods comprise tailing both the 5? and 3? ends of mature small RNA by ligating a 5? ligation adaptor to the 5? end and polyadenylating the 3? end. Other embodiments comprise reverse transcribing the adaptor ligated, polyadenylated mature small RNA with a universal reverse transcription primer and amplifying the cDNA with universal primers.Type: GrantFiled: January 13, 2020Date of Patent: March 15, 2022Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Chunmei Liu, Shoulian Dong, Linda Wong
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Patent number: 11274305Abstract: Methods and kits for excising HIV-1 DNA in vivo are provided, which employ Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-Associated (cas) proteins. Vectors harboring nucleic acids encoding one or more guide RNA, wherein said guide RNA hybridizes with a target HIV-1 DNA are also provided.Type: GrantFiled: March 25, 2014Date of Patent: March 15, 2022Assignees: TRUSTEES OF DARTMOUTH COLLEGE, United States Government as Represented by The Department of Veterans AffairsInventors: Alexandra L. Howell, Susan K. Eszterhas
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Patent number: 11268133Abstract: Disclosed herein are methods of detecting presence of a gene fusion in a sample from a subject. In some embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize a fusion probe that spans the point of fusion between two nucleic acids or genes. In other embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize two or more probes that flank the point of fusion between two nucleic acids or genes. In additional embodiments, the methods can include determining the percentage of gene fusion in the sample relative to the first nucleic acid or the second nucleic acid.Type: GrantFiled: April 8, 2019Date of Patent: March 8, 2022Assignee: HTG Molecular Diagnostics, Inc.Inventors: Bruce A. Seligmann, BJ Kerns, John Luecke, Matt Rounseville, Ihab Botros, Mark Schwartz
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Patent number: 11266990Abstract: A micro-fluidic device 100 for performing digital PCR is presented. The device comprises: a semiconductor substrate; a first micro-fluidic channel 104, comprising an inlet 102 and an outlet 103, embedded in the semiconductor substrate; a heating element 101 thermally coupled to the first micro-fluidic channel 104; a droplet generator 107 connected to the inlet 102 of the first micro-fluidic channel 104 for generating droplets and pumping generated droplets at a flow rate into the first micro-fluidic channel 104; characterized in that: the heating element 101 is a single heating element connected to a temperature control unit 111 configured to cycle the temperature of the complete first micro-fluidic channel 104 through at least two temperature values; and wherein the flow rate of the droplet generator 107 is adaptable. Further, a method to perform digital PCR is presented using the micro-fluidic device 100.Type: GrantFiled: August 9, 2018Date of Patent: March 8, 2022Assignee: IMEC VZWInventors: Paolo Fiorini, Tim Stakenborg, Frederik Colle
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Patent number: 11260386Abstract: The present disclosure relates to, inter alia, an easy-to-operate, fully closed component, which can be part of an instrument, for purification of biomolecules from biological samples, and subsequent transfer, and testing of the biomolecules, as well as an instrument comprising the component, and a method for using the component.Type: GrantFiled: June 5, 2015Date of Patent: March 1, 2022Assignee: THE EMERTHER COMPANYInventors: Yongmei Li, Li Li
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Patent number: 11260361Abstract: A device for synthesis of macromolecules is disclosed. In one aspect, the device comprises an ion-releaser having a synthesis surface comprising an array of synthesis locations arranged for synthesis of the macromolecules. The ion-releaser also includes an ion-source electrode, which is arranged to contain releasable ions and is arranged to be in contact with each of the synthesis locations of the synthesis surface, thereby release ions to the synthesis locations. The ion-releaser further comprises activating electrodes, which are arranged to be in contact with the ion-source electrode, wherein each one of the activating electrodes is arranged in association with one of the synthesis locations via the ion-source electrode. The ion-releaser is arranged to release at least a portion of the releasable ions from the ion-source electrode to one of the synthesis locations, by activation of the activating electrode associated with the synthesis location.Type: GrantFiled: May 14, 2019Date of Patent: March 1, 2022Assignee: IMEC VZWInventors: Philippe Vereecken, Brecht Put, Tim Stakenborg, Arnaud Furnemont, Luca Di Piazza
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Patent number: 11254953Abstract: The present disclosure relates to compositions and methods for investigating Zika virus (ZIKV) biology and pathogenicity. The present disclosure provides genetically stable viral vectors to produce functional RNA transcripts of ZIKV cDNAs. In particular, the present disclosure provides full-length infectious cDNAs as bacterial artificial chromosomes for spatiotemporally distinct and genetically divergent ZIKVs. The present disclosure also provides methods of generating a genetically engineered attenuated ZIKV using the genetically stable viral vectors described herein.Type: GrantFiled: April 18, 2019Date of Patent: February 22, 2022Assignee: Utah State UniversityInventor: Young-Min Lee
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Patent number: 11254989Abstract: The present disclosure provides methods and compositions of modified oligonucleotide primer and probe combinations, structurally modified with locked nucleic acids, quenchers, and dyes, effective to detect tumor-derived Human Papilloma Virus (HPV) and tumor-derived Epstein-Barr virus (EBV) and, especially, to distinguish viral DNA derived from tumors from viral DNA derived from infectious viral particles.Type: GrantFiled: March 16, 2021Date of Patent: February 22, 2022Assignee: The University of North Carolina at Chapel HillInventors: Gaorav Gupta, Bhishamjit S. Chera, Sunil Kumar
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Patent number: 11248272Abstract: Disclosed herein are methods for detecting presence of a target nucleic acid (such as an influenza virus nucleic acid) in a sample. In some embodiments, the methods include contacting the sample with a first probe capable of hybridizing to the target nucleic acid and a second probe capable of hybridizing to the target nucleic acid, contacting the resulting complex with one or more gap filling reagents, thereby producing a gap-filled target nucleic acid, isolating and amplifying the gap-filled target nucleic acid. The amplified gap-filled target nucleic acid covalently linked to the substrate is then detected, for example with a detectably labeled probe. Also disclosed herein are probes capable of hybridizing to influenza virus nucleic acids. The disclosure also includes kits for detecting and/or discriminating influenza virus nucleic acids in a sample. In some examples, the kits include two or more of the disclosed probes.Type: GrantFiled: June 23, 2017Date of Patent: February 15, 2022Assignee: The United States of America, as represented by the Secretary, Department of Health and Human ServicesInventors: Genyan Yang, Charles Todd Davis
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Patent number: 11248223Abstract: The present invention relates to a buffer system comprising a dicarboxylic acid or tricarboxylic acid or a salt thereof for synthesizing RNA molecules as well as a method of RNA in vitro transcription using this buffer system. The present invention also provides the use of this buffer system in RNA in vitro transcription and in the reduction or prevention of precipitates during RNA in vitro transcription.Type: GrantFiled: December 23, 2016Date of Patent: February 15, 2022Assignee: CureVac AGInventors: Benyamin Yazdan Panah, Aniela Wochner, Tilmann Roos, Andreas Funkner, Martin Kunze
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Patent number: 11249098Abstract: The present teachings relate to methods, systems, and kits for the preparation, purification and/or analysis of a glycan or glycoconjugate, and specifically to a magnetic bead based sample preparation protocol. In some aspects, the sample preparation protocol can provide for glycoconjugate capture, glycan release, fluorescent derivatization, and glycan purification for subsequent capillary electrophoresis, liquid chromatography, or other glycoanalytical method using magnetic beads containing negatively charged carboxyl groups extending from the surface of the magnetic beads.Type: GrantFiled: February 15, 2019Date of Patent: February 15, 2022Assignee: Beckman Coulter, Inc.Inventors: Andras Guttman, Clarence Lew, Marton Szigeti, Csaba Varadi
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Patent number: 11242520Abstract: Provided is a method for isolating exosomal DNA, the method comprising: (i) providing a sample, wherein the sample comprises a blood sample from peripheral blood of a pregnant woman; and (ii) subjecting the sample to isolating, thus obtaining the exosomal DNA. Also provided are a method for detecting a blood sample, a method for constructing a sequencing library on a blood sample, a method for high-throughput sequencing an exosomal DNA sequencing library, a method for non-invasive prenatal gene detection, a device for non-invasive prenatal gene detection, and a kit for detecting a blood sample and use thereof.Type: GrantFiled: July 23, 2019Date of Patent: February 8, 2022Assignees: BGI SHENZHEN, MGI TECH CO., LTD.Inventors: Ya Gao, Sen Lu, Jia Zhao, Peng Zeng, Fang Chen, Hui Jiang
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Patent number: 11236325Abstract: Solutions, reagents, and methods for nucleic acid purification. In certain aspects, cationic surfactant and, optionally, an anionic surfactant solutions are provided which can be used for phase separation and capture of nucleic acids, such as plasmid or genomic DNA, to a solid phase carrier, such as a mineral matrix.Type: GrantFiled: April 25, 2018Date of Patent: February 1, 2022Assignee: ZYMO RESEARCH CORPORATIONInventors: Ryan Kemp, Jonathan A. Claypool, Marc E. Van Eden, Xi-Yu Jia
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Patent number: 11230734Abstract: Some embodiments of the present application relate to novel modified nucleotide linkers for increasing the efficiency of nucleotide incorporation in Sequencing by Synthesis applications. Methods of preparing these modified nucleotide linkers are also provided herewith.Type: GrantFiled: January 25, 2019Date of Patent: January 25, 2022Assignee: Illumina Cambridge LimitedInventors: Xiaolin Wu, Xiaohai Liu
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Patent number: 11225655Abstract: The present invention is directed to a method for the synthesis of a bi-functional complex comprising a molecule part and an identifier oligonucleotide part identifying the molecule part. A part of the synthesis method according to the present invention is preferably conducted in one or more organic solvents when a nascent bi-functional complex comprising an optionally protected tag or oligonucleotide identifier is linked to a solid support, and another part of the synthesis method is preferably conducted under conditions suitable for enzymatic addition of an oligonucleotide tag to a nascent bi-functional complex in solution.Type: GrantFiled: April 16, 2011Date of Patent: January 18, 2022Assignee: NUEVOLUTION A/SInventors: Alex Haahr Gouliaev, Thomas Franch, Michael Anders Godskesen, Kim Birkebæk Jensen
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Patent number: 11224156Abstract: A single-grain seeder for introducing seeds into soil, including a singulating member which is suitable for singulating seeds supplied from a storage container and for dispensing the seeds individually, wherein the single-grain seeder has an application unit for applying a dressing agent to a singulated seed, wherein the application unit is arranged with respect to the singulating member in such a manner that the dressing agent is applied to a seed after the seed has been singulated and prior to the seed being dispensed from the singulating member.Type: GrantFiled: April 4, 2017Date of Patent: January 18, 2022Assignee: BAYER CROPSCIENCE AGInventors: Heinz-Friedrich Schnier, Carsten Conzen, Livia Assumpcao Moreira
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Patent number: 11220714Abstract: The present invention relates to a method of diagnosing cancer in a subject comprising detecting in the DNA of said subject at least one hypermethylated CpG island associated with said cancer, wherein an elevation in the level of methylation in said CpG island of said subject, relative to the level of methylation in said CpG island of a control subject, is indicative of said CpG island being hypermethylated.Type: GrantFiled: March 21, 2016Date of Patent: January 11, 2022Assignee: ERASMUS UNIVERSITY MEDICAL CENTER ROTTERDAMInventors: Ellen Catharina Zwarthoff, Annechiena Geertruide Van Tilborg
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Patent number: 11220705Abstract: The present invention is directed to a method for immobilizing nucleic molecule on solid support and to a use of a nucleic acid non-immobilized primer in combination with a nucleic acid primer linked to a solid support in said a method.Type: GrantFiled: April 25, 2016Date of Patent: January 11, 2022Assignee: QIAGEN GmbHInventors: Christian Korfhage, Evelyn Fricke