Involving Bacterium, Fungus, Parasite Or Protozoan (e.g., Detecting Pathogen Virulence Factors, Adhesions, Toxins, Etc.) Patents (Class 435/6.15)
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Publication number: 20130129682Abstract: The present invention provides isolated polypeptides having oligopeptide permease activity and an amino acid sequence that has at least 80% identity with a Haemophilus parasuis OppA polypeptide. Also provided by the present invention are isolated polynucleotides that encode the polypeptides described herein, and antibody that specifically binds a polypeptide described herein. The present invention further provides genetically modified microbes, such as attenuated Haemophilus parasuis strains and other microbes that express polypeptides described herein. Also included are methods for using the polypeptides, polynucleotides, antibody, and genetically modified microbes.Type: ApplicationFiled: November 4, 2010Publication date: May 23, 2013Applicant: Regents of the University of MinnesotaInventor: Simone Oliveira
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Patent number: 8445235Abstract: The current invention pertains to a method to produce RTX-toxins ApxI or ApxIII by culturing Actinobacillus pleuropneumoniae bacteria in a liquid culturing medium that supports growth of the bacteria, characterized in that air is passed through the medium, wherein the air has a carbon dioxide content above normal atmospheric level to increase RTX-toxin production during the production phase of the RTX-toxins.Type: GrantFiled: November 25, 2009Date of Patent: May 21, 2013Assignee: Intervet International B.V.Inventors: Simen-Jan Slagman, Christian Theodoor Gerardus Smits
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Patent number: 8445195Abstract: The present invention relates to the detection and identification of different bacterial species, all of which cause zoonosis, based on DNA analysis. More specifically, the invention provides the primers, probes, genes and genic regions required to apply a method for the simultaneous detection of bacteria and bacterial groups belonging to the genera Anaplasma, Ehrlichia, Borrelia, Bartonella, Coxiella, Rickettsia and Francisella based on Multiple PCR analysis by RLB (Reverse Line Blotting), in addition to providing a kit to carry out said analysis.Type: GrantFiled: June 15, 2006Date of Patent: May 21, 2013Assignee: Instituto de Salud Carlos IIIInventors: Pedro Anda Fernández, Raquel Escudero Nieto, Isabel Jado García, Isabel Rodríguez Moreno, Maria Isabel Jimenez Alonso
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Publication number: 20130122025Abstract: A method of quickly producing a vaccine for a biotype of pathogenic microorganism is described, where a nucleic acid molecule or fragment thereof is obtained from a biological sample from an animal exposed to the microorganism, a protective molecule is prepared based on the nucleic acid molecule of interest or fragment thereof, and administered to an animal which has been or is as risk of being exposed to the microorganism. A protective response to the biotype of the microorganism is obtained in the animal.Type: ApplicationFiled: January 8, 2013Publication date: May 16, 2013Applicant: HARRISVACCINES, INC.Inventor: Harrisvaccines, Inc.
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Publication number: 20130123543Abstract: The present invention relates to isolated nucleic acid sequences involved in, or capable of the biosynthesis of polyacetylenic compounds with anti-fungal activity and especially isolated nucleic acid sequences derived from Collimonas fungivorans. Further, the present invention relates to methods for identifying the present isolated nucleic acids, the use thereof for identification of homologous nucleic acids in other organisms or the use thereof for the biosynthesis of polyacetylenic compounds with anti-fungal activity. Further, the present invention relates to novel polyacetylenic compounds with anti-fungal activity. Specifically, the present invention relates to isolated nucleic acids encoding one or more proteins involved in the synthesis of a polyacetylenic compound with antifungal activity wherein said isolated nucleic acid comprises a nucleic acid sequence having at least 70%, preferably at least 80%, more preferably at least 90%, most preferably at least 95% identity with SEQ ID No. 1.Type: ApplicationFiled: April 14, 2010Publication date: May 16, 2013Applicant: NETHERLANDS INSTITUTE OF ECOLOGYInventors: Kathryn Fritsche, Johannes Henricus Josephus Leveau, Wietse De Boer, Johannes Antonie Van Veen
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Patent number: 8440808Abstract: RpoB gene sequences of various species of Acinetobacter bacteria, and a method of detection by molecular identification of various species of Acinetobacter bacteria using rpoB gene sequences.Type: GrantFiled: March 16, 2006Date of Patent: May 14, 2013Assignees: Universite d'Aix-Marseille, Centre National de la Recherche Scientifique (CNRS)Inventors: Bernard La Scola, Didier Raoult
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Publication number: 20130109590Abstract: The invention generally relates to using magnetic particles and magnets to isolate a target analyte from a sample. In certain embodiments, methods of the invention involve introducing to a sample at least one set of magnetic particles having at least one type of lectin in order to create a mixture, incubating the mixture such that the lectin binds to a carbohydrate on a surface of a target in the sample to thereby form target/particle complexes, in which the incubating is performed in the presence of a buffer that influences binding, and applying a magnetic field to separate target/particle complexes from remaining components of the sample, thereby isolating target/magnetic particle complexes.Type: ApplicationFiled: December 19, 2012Publication date: May 2, 2013Inventors: Lisa-Jo Ann Clarizia, Eddie W. Adams, Sergey A. Dryga
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Patent number: 8426137Abstract: Compositions and methods for the detection of vancomycin-resistant pathogens using primers and/or probes to the vanA and vanB genes.Type: GrantFiled: July 5, 2011Date of Patent: April 23, 2013Assignee: Genohm Sciences Canada, Inc.Inventors: Michel G. Bergeron, Maurice Boissinot, Ann Huletsky, Christian Menard, Marc Ouellette, Francois J. Picard, Paul H. Roy
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Publication number: 20130095094Abstract: The invention provides assays and apparatuses for assessing the ability of certain orally-ingestible samples, such as saliva or foodstuffs to cause inflammation, and thus the health risk such samples can cause upon ingestion by a subject. The invention also provides an assay to monitor an individual's diet with regards to inflammation risk. The invention also extends to methods of preventing inflammatory diseases.Type: ApplicationFiled: June 9, 2011Publication date: April 18, 2013Applicant: University of LeicesterInventor: Clett Ben Erridge
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Publication number: 20130089874Abstract: The present invention provides a variety of microfluidic devices and methods for conducting assays and syntheses. The devices include a solid substrate layer having a surface that is capable of attaching ligand and or anti-ligand, and an elastomeric layer attached to said surface. Preferred embodiments have deflectable membrane valves and pumps, for example, rotary pumps associated therewith.Type: ApplicationFiled: March 29, 2012Publication date: April 11, 2013Applicant: Fluidigm CorporationInventors: Ian David Manger, Joseph W. Barco, Hany Ramez Nassef
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Patent number: 8415105Abstract: The invention relates to a method of detecting pathogenic Legionella pneumophila strains by hybridizing genomic DNA of a sample suspected to contain Legionella to two or five specific sequence markers, as identified by MARKER NO. 1 through MARKER NO. 5 or homologues thereof. The invention further relates to a kit of parts comprising an array and reference materials for performing a method of the invention.Type: GrantFiled: December 28, 2007Date of Patent: April 9, 2013Assignee: Nederlandse Organisatie voor toegepast-natuurwetenschappelijk Onderzoek TNOInventors: Frank Henri Johan Schuren, Roy Christiaan Montijn, Henricus Matheus Wilhelmus Maria Thijssen
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Publication number: 20130078633Abstract: The invention provides a non-invasive technique for the detection and quantification of immunoglobulin isotypes, in a biological sample containing a plurality of distinct cell populations. Methods are conducted using sequencing technology to detect and enumerate immunoglobulin isotype profiles within a heterogeneous biological sample.Type: ApplicationFiled: September 24, 2012Publication date: March 28, 2013Applicant: ImmuMetrix, LLCInventor: ImmuMetrix, LLC
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Patent number: 8399242Abstract: The present invention relates to methods for the production of ?-3 and/or ?-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to ?-linolenic acid (GLA); ?-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-?-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to arachidonic acid (ARA); ETA to eicosapentaenoic acid (EPA); DGLA to ETA; EPA to docosapentaenoic acid (DPA); and ARA to EPA have been introduced into the genome of Yarrowia for synthesis of ARA and EPA.Type: GrantFiled: May 20, 2009Date of Patent: March 19, 2013Assignee: E I du Pont de Nemours and CompanyInventors: Stephen K. Picataggio, Narendra S. Yadav, Quinn Qun Zhu
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Publication number: 20130059313Abstract: A method for quantitative characterization of non-covalent interactions and analyte quantification in nanoliter samples is described. The procedure is based on Flow Induced Dispersion Analysis (FIDA), of which the only system requirements is a narrow tube, capillary or channel equipped with a detector. The technique can be implemented using standard equipment such as High Performance Liquid Chromatography (HPLC), Flow Injection Analysis (FIA) or Capillary Electrophoresis (CE).Type: ApplicationFiled: February 25, 2011Publication date: March 7, 2013Applicant: FIDA-TECH APSInventors: Henrik Jensen, Jesper Ostergaard
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Publication number: 20130052645Abstract: This invention describes a method for identifying bacteria. In particular, this invention relates to a method for identifying and quantifying mycobacteria from a sputum sample taken from a subject using flow cytometry. Further described is the use of flow cytometry to identify and quantify Mycobacterium tuberculosis from sputum-derived samples. Once identified and quantified, the samples are spotted onto filter cards for use in verification of an existing method of diagnosis, calibration of an existing method of diagnosis and/or the establishment of an external quality control system for use in conjunction with these methods of diagnosis. In one embodiment the method is used to diagnose tuberculosis (TB).Type: ApplicationFiled: May 6, 2011Publication date: February 28, 2013Applicant: University of the Witwatersrand, JohannesburgInventor: Lesley Erica Scott
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Publication number: 20130053277Abstract: Methods of identifying inhibitors of polypeptide-of-interests are provided.Type: ApplicationFiled: May 4, 2011Publication date: February 28, 2013Applicant: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.Inventor: Isaiah Arkin
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Publication number: 20130045482Abstract: The present invention relates to novel bacteria and the uses thereof. The invention particularly relates to Deinococcus bacteria and their use in the pharmaceutical or agro-chemical industries, e.g., for degrading biomass and/or producing metabolites or drugs of industrial interest.Type: ApplicationFiled: August 31, 2012Publication date: February 21, 2013Applicant: DEINOVEInventors: JEAN-MICHEL CLAVERIE, Jacques Biton, Jean-Paul Leonetti
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Publication number: 20130040288Abstract: Disclosed are compositions for isolating populations of nucleic acids from biological, forensic, and environmental samples. Also disclosed are methods for using these compositions as one-step formulations for killing pathogens, inactivating nucleases, and releasing polynucleotides from other cellular components within the sample, and stabilizing the nucleic acids prior to further processing or assay. The disclosed compositions safely facilitate rapid sample collection, and provide extended storage and transport of the samples at ambient or elevated temperature without contamination of the sample or degradation of the nucleic acids contained therein. This process particularly facilitates the collection of specimens from remote locations, and under conditions previously considered hostile for preserving the integrity of nucleic acids released from lysed biological samples without the need of refrigeration or freezing prior to molecular analysis.Type: ApplicationFiled: October 1, 2012Publication date: February 14, 2013Applicant: Longhorn Vaccines & Diagnostics, LLC.Inventor: Longhorn Vaccines & Diagnostics, LLC.
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Publication number: 20130040305Abstract: Embodiments herein report compositions, systems and methods for making and using plasmid vectors and nanotube complexes. In certain embodiments, compositions, systems and methods herein include making plasmid vectors having aptamer inserts. In some embodiments, methods disclosed herein may be used to rapidly generate large quantities of plasmid vectors having aptamer inserts directed to a particular target agent. Other aspects concern plasmid constructs associated with organic semiconductors. Yet other aspects concern complexes of nanotubes associated with dsDNA aptamers and tracking molecules.Type: ApplicationFiled: September 24, 2012Publication date: February 14, 2013Inventors: Johnathan L. KIEL, Amanda TIJERINA, Eric A. HOLWITT, Jill PARKER, Mark SLOAN, Melanie WOITASKE, Maomian FAN
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Patent number: 8367346Abstract: The invention provides biosynthetic routes to xylitol production that do not require pure D-xylose for synthesis and that can utilize inexpensive substrates such as hemicellulose hydrolysates.Type: GrantFiled: May 11, 2011Date of Patent: February 5, 2013Assignees: Biotechnology Research Development Corporation, The United States of America, as represented by Sec. of AgricultureInventors: Paul Taylor, Nathan Wymer, Francis Michael Racine
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Publication number: 20130029863Abstract: The invention relates to the diagnostic field and to the characterization of antibiotic-resistant bacteria in a sample by detecting specific nucleotides in the CMY-2 gene.Type: ApplicationFiled: September 30, 2010Publication date: January 31, 2013Applicants: Institut Pasteur, Assistance Publique - Hopitaux de ParisInventors: Etienne Ruppe, Antoine Andremont, Raymond Ruimy, Patrice Courvalin, Sylvie Bremont
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Publication number: 20130029347Abstract: The present invention generally relates to devices and methods for immobilizing nucleic acids on a substrate. In certain embodiments, devices of the invention include a voltage source, and a substrate coupled to the voltage source, in which hydrophobicity of the substrate changes in response to an applied electric field and a surface of the substrate is coated with a substance that retains nucleic acids.Type: ApplicationFiled: July 26, 2011Publication date: January 31, 2013Applicant: OPGEN, INC.Inventor: Wenlong Jiang
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Publication number: 20130029348Abstract: The invention generally relates to methods for elongating nucleic acid, such as DNA, on a charged substrate. In certain embodiments, methods of the invention involve contacting an applicator to a sample including a nucleic acid, and swabbing the applicator on a charged substrate, thereby elongating the nucleic acid on the substrate.Type: ApplicationFiled: July 26, 2011Publication date: January 31, 2013Applicant: OPGEN, INC.Inventors: Ryan Nathan Ptashkin, Wenlong Jaing
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Patent number: 8361737Abstract: The invention is a herd management schema based upon the inventor's analysis of the natural history of bovine infection due to Mycobacterium avium subspecies paratuberculosis (Map) and related genomic variants and upon the ability of two distinct Map ELISA tests to sequentially or in parallel determine prior and current Map infection and evidence of active mycobacterium replication. Interpretation of the test results are integrated into sequential directives designed to enhance productive retention of infected animals as well as identify animals not previously infected. The sequential utilization of the data guidelines is developed to minimize the adverse economic impact.Type: GrantFiled: March 8, 2012Date of Patent: January 29, 2013Inventor: Gilles R. G. Monif
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Patent number: 8362227Abstract: The invention provides methods to detect C. difficile in biological samples using real-time PCR. Primers and probes for the detection of C. difficile are provided by the invention. Articles of manufacture containing such primers and probes for detecting C. difficile are further provided by the invention.Type: GrantFiled: January 9, 2012Date of Patent: January 29, 2013Assignee: Mayo Foundation for Medical Education and ResearchInventors: Franklin R. Cockerill, III, Thomas F. Smith, Jon E. Rosenblatt, Lynne M. Sloan
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Publication number: 20130022547Abstract: The present invention provides live, attenuated Mycoplasma gallisepticum bacteria that exhibit reduced expression of a protein identified as MGA_0621. In certain embodiments, the attenuated bacteria may additionally exhibit reduced expression of one or more proteins selected from the group consisting of pyruvate dehydrogenase, phosphopyruvate hydratase, 2-deoxyribose-5-phosphate aldolase, and ribosomal protein L35, relative to a wild-type M. gallisepticum bacterium. Also provided are vaccines and vaccination methods involving the use of the live, attenuated M. gallisepticum bacteria, and methods for making live attenuated M. gallisepticum bacteria. An exemplary live, attenuated strain of M. gallisepticum is provided, designated MGx+47, which was shown by proteomics analysis to exhibit significantly reduced expression of MGA_0621, and was shown to be safe and effective when administered as a vaccine against M. gallisepticum infection in chickens.Type: ApplicationFiled: September 26, 2012Publication date: January 24, 2013Applicant: Wyeth LLCInventors: Mahesh KUMAR, Muhammad Ayub Khan
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Patent number: 8357791Abstract: The DNA of the invention are characterized in that they concern the whole or part of genes, with their reading frame, to be found in Neisseria meningitidis, but not in Neisseria gonorrhoeae, or in Neisseria lactamica except the genes involved in the biosynthesis of the polysaccharide capsule, frp A, frp C, opc, por A, rotamase the sequence IC1106, IgA protease, pilline, pilC, transferrin binding proteins and opacity proteins. The invention also concerns the polypeptides corresponding to these DNA and the antibodies directed against these polypeptides. It is applicable in the prevention and the detection of meningococcus induced infections and meningitis.Type: GrantFiled: January 20, 2011Date of Patent: January 22, 2013Assignee: Institut National de la Sante et de la Recherche Medicale (INSERM)Inventors: Xavier Nassif, Colin Tinsley
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Patent number: 8357504Abstract: Method for the quantitative determination of vitamins, amino acids, or other substances necessary for life, in a substance mixture, whereby in the culture container, the cavities of a microtitration plate, in each case a predetermined number of vital cells of a suitable microorganism are prepared in a permanent manner. For this purpose, the cells are shock frozen at ?80° C. and then freeze dried in a freezing solution containing 200 to 500 mM trehalose/sucrose. The freezing solution is preferably the test medium. The culture containers are preferably the wells of a microtitration plate.Type: GrantFiled: August 1, 2005Date of Patent: January 22, 2013Assignee: IFP Privates Institut fur Produktqualitat GmbHInventors: Wolfgang Weber, Franz Paul Armbruster
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Patent number: 8357493Abstract: The invention concerns the isolation of nucleotide and peptide sequences in particular for differentiating, in diagnostic terms, an immunization resulting from BCG vaccination of an infection by M. tuberculosis. Said sequences are either M. bovis BCG/M. bovis specific or M. tuberculosis specific. The invention also concerns a method for detecting said sequences, a method for detecting antibodies generated by the products expressing said sequences as well as kits for implementing said methods. Finally, the invention concerns novel vaccines.Type: GrantFiled: November 4, 2004Date of Patent: January 22, 2013Assignee: Institut PasteurInventors: Stewart Cole, Stéphen Gordon, Roland Buchrieser-Brosch, Alain Billault, Thierry Garnier
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Publication number: 20130017552Abstract: The invention relates to a method for at least partially separating viral and/or prokaryotic nucleic acids from eukaryotic nucleic acids from a biological sample, in particular a eukaryotic cell suspension, comprising the following steps in the following order: a) re-suspending the cells in the presence of a chelating agent, in particular EDTA or EDTA in combination with a saccharide, b) lysis of the cells by chemical lysis, such as alkaline lysis, enzymatic lysis and/or boiling lysis and/or mechanical lysis, c) neutralizing the cell lysate and d) separating the precipitated eukaryotic nucleic acids and obtaining the viral and/or prokaryotic nucleic acids, as well as the use of a kit comprising a re-suspension buffer with chelating agent and optionally a saccharide and RNAse, lysis buffer comprising at least one base and a detergent and neutralizing buffer for at least partially separating viral and/or prokaryotic nucleic acids from eukaryotic nucleic acids from a biological sample, in particular a eukaryoticType: ApplicationFiled: April 7, 2011Publication date: January 17, 2013Applicant: GREINER BIO - ONE GMBHInventor: Walter Rudorfer
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Publication number: 20130017214Abstract: The BAA antigen of Staphylococcus aureus was identified in a highly bacteremic MRSA strain that has enhanced in vitro binding to fibronectin and other extracellular matrix proteins compared with other endemic and related strains. BAA is a phage-encoded surface-expressed adhesin which binds fibronectin in vitro. It may be responsible for the enhanced catheter-related bacteremic phenotype of MRSA strains and is thus useful as a vaccine target to prevent MRSA bacteremia.Type: ApplicationFiled: November 9, 2010Publication date: January 17, 2013Applicant: GUY'S AND ST THOMAS'S NHS FOUNDATION TRUSTInventor: Jonathan Edgeworth
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Publication number: 20130017203Abstract: The present invention relates to methods and compositions for preventing and treating Staphylococcus aureus in a subject. Therapeutic compositions of the present invention comprise leukocidin E and/or D proteins or polypeptides and anti-leukocidin E and/or D antibodies. The invention further relates to methods of identifying inhibitors of LukE/D cytotoxicity and inhibitors of LukE/D-leukocyte binding.Type: ApplicationFiled: June 19, 2012Publication date: January 17, 2013Applicant: NEW YORK UNIVERSITYInventors: Victor J. TORRES, Francis ALONZO, III
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Publication number: 20130011828Abstract: The present invention relates to the use of one or more cas genes for modulating resistance in a cell against a target nucleic acid or a transcription product thereof.Type: ApplicationFiled: June 6, 2012Publication date: January 10, 2013Inventors: Rodolphe Barrangou, Patrick Boyaval, Christophe Fremaux, Philippe Horvath, Dennis Romero
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Publication number: 20130011428Abstract: The present invention relates to newly identified open reading frames comprised within the genomic nucleotide sequence of Streptococcus pneumoniae, wherein the open reading frames encode polypeptides that are surface localized on Streptococcus pneumoniae. Thus, the invention relates to Streptococcus pneumoniae open reading frames that encode polypeptides encoded by the Streptococcus pneumoniae open reading frames, vectors comprising open reading frame sequences and cells or animals transformed with these vectors. The invention relates also to methods of detecting these nucleic acids or polypeptides and kits for diagnosing Streptococcus pneumoniae infection. The invention finally relates to pharmaceutical compositions, in particular immunogenic compositions, for the prevention and/or treatment of bacterial infection, in particular infections with Streptococcus pneumoniae.Type: ApplicationFiled: July 11, 2012Publication date: January 10, 2013Applicant: WYETH HOLDINGS CORPORATIONInventors: Robert John Zagursky, Amy Wadhams Masi, Bruce Arthur Green, Deb Narayan Chakravarti, David Parrish Russell, Joseph Lawrence Wooters
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Patent number: 8349582Abstract: Exemplary embodiments provide bioaerosol detection systems and methods for detecting bioaerosols. In one embodiment, the bioaerosol detection system can include a humidifier to increase the humidity of a continuously flowing sample volume of a bioaerosol sample using a biologically compatible liquid medium, and an amplifier to deposit vapor on the bioaerosol sample for a particle size amplification process. Bioaerosol(s) can thus be detected and sampled while simultaneously maintaining their viability. The disclosed bioaerosol detection systems and the methods can provide high efficiency for sampling and detecting ultrafine bioaerosol(s) such as viruses and proteins, which can be smaller than 0.3 ?m in diameter and can be as small as 20 nm.Type: GrantFiled: August 15, 2008Date of Patent: January 8, 2013Assignee: University of Florida Research Foundation, Inc.Inventors: Chang-Yu Wu, Alexandros Demetrios Theodore, Jin-Hwa Lee, Lindsey Ann Riemenschneider
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Publication number: 20130005595Abstract: The present invention discloses an oligonucleotide which comprises a part or the entire sequence of the nucleotide sequence shown in SEQ ID NOS: 1-8, or a part or the entire sequence of a sequence complementary to the nucleotide sequence shown in SEQ ID NOS: 1-8, wherein the oligonucleotide is capable of hybridizing with a nucleotide sequence of Mycobacterium intracellulare gene; a primer or a probe for the detection of M. intracellulare, which comprises the oligonucleotide; and a method for detection of M. intracellulare using the primer and/or the probe. According to the detection method, false-positive results can be eliminated and detection of M. intracellulare can be carried out with higher accuracy, greater precision, and greater specificity compared to a conventional diagnostic method employing a cell culture assay or a PCR assay. The method also enables to quantify a microbial cell.Type: ApplicationFiled: May 23, 2012Publication date: January 3, 2013Inventor: Tomokazu Ishikawa
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Publication number: 20130004942Abstract: A first intracellular pathogen in a biological sample that may contain more than one intracellular pathogen is studied by a method comprising the steps of (i) contacting the sample with a population of cells in the presence of an agent inhibiting the reproduction of a second intracellular pathogen; (ii) incubating the cells under conditions that permit the reproduction of the first intracellular pathogen; and (iii) testing material arising from step (ii) for the first intracellular pathogen.Type: ApplicationFiled: March 7, 2011Publication date: January 3, 2013Applicant: NOVARTIS AGInventor: Theodore Tsai
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Patent number: 8343726Abstract: The present invention provides assays and devices for detection of substances in liquid samples. The assays and devices utilize passive diffusion between a porous material and a porous membrane containing a specific binding pair member to enable detection of the substance of interest.Type: GrantFiled: November 23, 2005Date of Patent: January 1, 2013Assignee: Techlab, Inc.Inventors: James H. Boone, David M. Lyerly, Tracy D. Wilkins
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Publication number: 20120329059Abstract: A method for producing bacteria belonging to the genus Bifidobacterium having excellent viability even under various conditions with different environmental factors, novel bacteria belonging to the genus Bifidobacterium obtained by the method, and a method for detecting the bacteria are provided. By subculturing and storing bacteria belonging to the genus Bifidobacterium alternately in systems under conditions with different environmental factors, the bacteria belonging to the genus Bifidobacterium exhibiting excellent viability under all the conditions used for the alternate subculturing and storing can be produced.Type: ApplicationFiled: February 21, 2011Publication date: December 27, 2012Applicant: KABUSHIKI KAISHA YAKULT HONSHAInventors: Tomoyuki Sako, Mika Miura, Yasuhisa Shimakawa, Koji Miyazaki, Junji Fujimoto, Koichi Watanabe
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Patent number: 8338107Abstract: The present invention relates to a method for isolating from the immunological gene repertoire a gene coding for a receptor having the ability to bind a preselected ligand. Receptors produced by the gene isolated by the method, particularly catalytic receptors, are also contemplated.Type: GrantFiled: November 16, 2006Date of Patent: December 25, 2012Assignees: Scripps Research Institute, Medical Research Institute, Catalyst Assets LLCInventors: William D. Huse, Gregory P. Winter, Lutz Riechmann, Joseph A. Sorge, Richard A. Lerner
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Patent number: 8329402Abstract: The present invention relates to a new combination of marker genes for characterizing a Lactobacillus sakei strain. In particular, the present invention concerns the use of a pattern of presence or absence of marker genes in the genome of the strain to be characterized for classifying and identifying said strain.Type: GrantFiled: February 20, 2009Date of Patent: December 11, 2012Assignee: Institut National de la Recherche AgronomiqueInventors: Stéphane Chaillou, Monique Zagorec, Marie-Christine Champomier-Verges
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Patent number: 8329884Abstract: This invention provides compositions and methods for detecting Neisseria gonorrhoeae in a sample. This invention also provides related reaction mixtures, kits, systems, and computers.Type: GrantFiled: November 10, 2011Date of Patent: December 11, 2012Assignee: Roche Molecular Systems, Inc.Inventors: Diane Kawa, Shi-Da Lu, Peter Dailey
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Publication number: 20120309000Abstract: The present disclosure provides an isolated DNA molecule derived from Mycobacteria having a DNA mismatch repair function represented by a nucleic acid sequence as disclosed in SEQ ID NO: 1 or 2. Also provided is a promoter sequence having SEQ ID NO: 3, and a recombinant vector comprising the isolated DNA of the present disclosure and a promoter operatively linked to the DNA molecule. The isolated DNA molecule of the present disclosure is classified as MutS4A and MutS4 and confers cells with resistance to UV and macrophages when transformed into the cells. Further it increases the frequency of homologous recombination and the genetic stability of a heterologous plasmid in cells.Type: ApplicationFiled: May 9, 2012Publication date: December 6, 2012Applicant: SNU R&DB FoundationInventors: Bum-Joon Kim, Byoung-Jun Kim
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Patent number: 8323901Abstract: This invention relates to novel nucleic acid-based probes and methods for detecting Plasmodium parasites in biological samples as well as detecting different Plasmodium parasites selectively from one another.Type: GrantFiled: October 29, 2009Date of Patent: December 4, 2012Assignee: ID-Fish Technology, Inc.Inventors: Jyotsna S. Shah, Danuta Wronska, Helena Weltman, Nick Harris, Suzanne Scherini-Ward, Olivia Mark
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Publication number: 20120302940Abstract: The present invention provides multifunctional popcorn-shaped gold nanomaterial for sensing, treatment and for a unique way of monitoring treatment effectiveness during therapy process of different human cancer cells and pathogenic bacteria. It consists of the following steps 1) Synthesis of popcorn-shaped gold nanoparticles of different sizes. 2) Design of multifunctional popcorn-shaped gold nanoparticle for targeted sensing, therapy and monitoring therapy effectiveness for different human cancer cells (liver, breast, skin and prostate) and drug resistance bacteria. 3) Design of portable SERS sensor for cancer detection, treatment and for monitoring treatment effectiveness using the same instrument. and 4) Application of our approach to detect and kill MDRB from food sample.Type: ApplicationFiled: November 30, 2011Publication date: November 29, 2012Applicant: JACKSON STATE UNIVERSITYInventor: Paresh Chandra Ray
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Patent number: 8318438Abstract: We describe examples using aptamers for capturing and reporting the presence of a target, such as a pathogen. Examples described here include a set of aptamers that are specific to F. tularensisis. Other examples described here include an Aptamer-Linked Immobilized Sorbent Assay (ALISA) and dot blot assay. An example of a method provided here comprises: providing a set of DNA sequences that exhibit high binding affinity to target antigen, placing the DNA sequences in a sandwich aptamer-linked immobilized sorbent assay (ALISA), contacting the DNA sequences with a sample, and detecting whether the target is present in the sample. Some alternative implementations may include dot blots and different reporters. Quantum dot sandwich assays and quantum dot de-quenching reporters can be used.Type: GrantFiled: February 27, 2008Date of Patent: November 27, 2012Assignee: The United States of America as represented by the Secretary of the Air ForceInventors: Jeevalatha Vivekananda, Johnathan L. Kiel
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Patent number: 8313911Abstract: Methods for the production of omega-3 and/or omega-6 fatty acids in oleaginous yeasts grown on a fermentable carbon source selected from the group consisting of invert sucrose, glucose, fructose and combinations of these, provided that glucose is used in combination with invert sucrose and/or fructose. Specifically, methods are provided for production of linoleic acid, eicosadienoic acid, gamma-linolenic acid, dihomo-gamma-linolenic acid, arachidonic acid, n-6 docosapentaenoic acid, ?-linolenic acid, stearidonic acid, eicosatrienoic acid, eicosatetraenoic acid, eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid.Type: GrantFiled: December 18, 2009Date of Patent: November 20, 2012Assignee: E I du Pont de Nemours and CompanyInventors: Ethel Noland Jackson, David Richard Short, Dongming Xie, Zhixiong Xue, Quinn Qun Zhu
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Patent number: 8313894Abstract: Methods and compositions for identifying antigens of human lymphocytes are provided herein.Type: GrantFiled: July 1, 2009Date of Patent: November 20, 2012Assignee: Genocea Biosciences, Inc.Inventors: Jessica Flechtner, Todd Gierahn
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Publication number: 20120282624Abstract: This invention includes a process for the identification and validation of a neutral polynucleotide integration site within the S. spinosa genome. In addition, the invention includes the use of the neutral site and methods for the integration of a polynucleotide containing a gene expression cassette, which is stably maintained and expressed over subsequent generations. The invention includes neutral integration sites that can be disrupted without negatively impacting spinosyn production, growth or other desired metabolic characteristics.Type: ApplicationFiled: May 3, 2011Publication date: November 8, 2012Applicant: DOW AGROSCIENCES LLCInventors: Lei Han, Nigel Mouncey
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Publication number: 20120282181Abstract: Methods for detecting an infection with Mtb in a subject are disclosed. The methods include detecting the presence of CD8+ T cells that specifically recognize an Mtb polypeptide. The methods include in vitro assays for detecting the presence of CD8+ T cells in a biological sample, and in vivo assays that detect a delayed type hypersensitivity reaction. The methods also include detecting Mtb polypeptides and polynucleotides.Type: ApplicationFiled: November 19, 2010Publication date: November 8, 2012Inventors: David M. Lewinsohn, Deborah A. Lewinsohn