To Identify An Enzyme Or Isoenzyme Patents (Class 435/7.4)
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Publication number: 20140348822Abstract: The invention provides a method for treating a medical condition, disease, or disorder mediated by a misfolded form of superoxide dismutase (SOD) in a subject in need of treatment. The method optionally comprises administering to the subject a composition comprising a pharmaceutically acceptable vehicle and an agent selected from (1) an exogenous antibody or fragment thereof that binds selectively to the misfolded form of SOD, and/or (2) an immunogen that elicits production of an endogenous antibody that binds selectively to the misfolded form of SOD, and/or (3) a nucleic acid sequence encoding (1) or (2). In certain embodiments, the invention provides methods of treating diseases such as Alzheimer's Disease, Parkinson's Disease or amyotrophic lateral sclerosis using amyotrophic disease-specific epitopes, and compositions including these epitopes. The invention also provides antibodies that bind to monomeric or misfolded SOD1, and not on the molecular surface of native homodimeric SOD1.Type: ApplicationFiled: June 10, 2014Publication date: November 27, 2014Inventors: Neil R. Cashman, Avijit Chakrabartty, Rishi Rakhit, Joachim Bernhard Ostermann
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Publication number: 20140349278Abstract: A device and method for detecting and assessing the quantity of a biological, biochemical, or chemical analyte in a test sample using a simple light source and the naked eye are disclosed. In one embodiment, the device comprises a nanohole sensor chip with two sections, the first of which is a test section, upon which capture agents for a particular analyte are immobilized, and the second of which is a reference section, upon which capture agents conjugated with known quantities of the analyte are immobilized. In another embodiment of the invention, a nanohole sensor chip with a test section and a plurality of reference sections is disclosed. The sensor utilizes light intensity changes exhibited by Fano resonances in the nanoholes for detection of analytes, and allows comparison between the light intensity changes between the reference sections and the test sections for assessing the quantity of the analyte in the sample.Type: ApplicationFiled: November 21, 2012Publication date: November 27, 2014Applicant: KONICA MINOLTA LABORATORY U.S.A., INC.Inventor: Noriaki Yamamoto
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Publication number: 20140348821Abstract: Methods of testing to identify and to treat a subset of colon cancer patients exhibiting dMMR tumor tissue, who derive significant clinical benefit from the addition of bevacizumab to standard adjuvant chemotherapy. The presence of a V600E BRAF mutation is also of significance.Type: ApplicationFiled: May 23, 2014Publication date: November 27, 2014Applicant: NSABP Foundation, Inc.Inventors: Katherine Lea Pogue-Geile, Soonmyung Paik
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Publication number: 20140349279Abstract: The present invention relates to a three dimensional (or 3D) microfluidic system comprising a plurality of layers stacked upon each other, characterised in that at least one of said layers consists of a 1st and at least one 2nd parts, distinct from each other, with the 2nd part being porous and wettable by a solution of interest, nesting into a recess of the 1st part being non-porous and/or non-wettable by said solution of interest, wherein said system can possibly have a built-in reservoir; a method for manufacturing the same and different uses thereof.Type: ApplicationFiled: December 14, 2012Publication date: November 27, 2014Inventors: Thomas Berthelot, Hervé Volland
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Publication number: 20140348833Abstract: The invention relates to novel methods for the treatment of tumors, comprising administration of a bispecific antibody or a combination of two or more non-cross-blocking antibodies that recognize the same target antigen or antigenic complex. In particular, the invention relates to a method for inducing complement-mediated cell killing in the treatment of a tumor, said method comprising combined administration, to a human being in need thereof, of a first antibody and a second antibody, wherein said first antibody binds EGFR, said second antibody binds EGFR, said first and second antibody are non-cross-blocking, and the dosage regimen is such that CDC is obtained at the tumor site.Type: ApplicationFiled: March 6, 2014Publication date: November 27, 2014Applicant: GENMAB A/SInventors: Jan VAN DE WINKEL, Paul PARREN, Willem Karel BLEEKER, Klaus EDVARDSEN, Jeroen Lammerts VAN BUEREN, Thomas VALERIUS, Michael DECHANT, Wencke WEISNER, Sven BERGER
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Publication number: 20140350047Abstract: The present invention concerns methods and compositions related to type 3 phosphodiesterases (PDE3). Certain embodiments concern isolated peptides corresponding to various PDE3A isoforms and/or site-specific mutants of PDE3A isoforms, along with expression vectors encoding such isoforms or mutants. In specific embodiments, methods for identifying isoform-selective inhibitors or activators of PDE3 are provided, along with methods of use of such inhibitors or activators in the treatment of dilated cardiomyopathy, pulmonary hypertension and/or other medical conditions related to PDE3 effects on cAMP levels in different intracellular compartments.Type: ApplicationFiled: March 27, 2014Publication date: November 27, 2014Applicants: University of Utah Research Foundation, The United States Government, as represented by the Department of Veterans AffairsInventor: Matthew A. Movsesian
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Publication number: 20140342373Abstract: The present invention concerns a microfluidic system comprising: a microchannel containing several elements of two non-miscible fluids, the microchannel comprising a droplet (30) containing magnetic particles (M), and a device for generating inside the microchannel magnetic field, said device comprising an activable magnetic element, the activable magnetic element comprising a tip (5,6), the microfluidic system being configured to transport the droplet by flow or by pressure difference.Type: ApplicationFiled: July 24, 2012Publication date: November 20, 2014Applicant: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUEInventors: Jean-Louis Viovy, Laurent Malaquin, Stefano Begolo, AnaïS Ali Cherif, Stephanie Descroix
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Publication number: 20140341927Abstract: The present invention concerns an antibody which specifically binds to an abnormal superoxide dismutase 1 (SOD1), and which neutralizes its pathologic effect when administered to an animal such as a human. The antibody of the invention is a monoclonal antibody produced by hybridoma cell lines deposited with the International Depositary Authority of Canada on Aug. 29, 2006 under accession numbers ADI-290806-01, ADI-290806-02 and ADS-290806-03. The present invention also concerns the use of the antibody of the invention in the treatment, prevention and diagnosis of neurodegenerative diseases such as Amyotrophic lateral sclerosis, Parkinson and Alzheimer in an animal such as a human.Type: ApplicationFiled: May 27, 2014Publication date: November 20, 2014Applicant: Universite LavalInventors: Jean-Pierre Julien, Makoto Urushitani
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Publication number: 20140336073Abstract: A method for determining the sensitivity and/or resistance of a patient suffering from a cancer disease to Aurora kinase inhibitor therapy, which comprises determining in vitro in the cancer cells or body fluids taken from the patient the expression of at least one gene selected from a particular group and/or determining in vitro in the cancer cells or body fluids taken from the patient the level of at least one protein selected from a particular group.Type: ApplicationFiled: December 7, 2012Publication date: November 13, 2014Applicants: PALACKY UNIVERSITY, OLOMOUC, INSTITUTE OF ANIMAL PHYSIOLOGY AND GENETICS ASCR, V.V.I.Inventors: Madhusudhan Reddy Kollaredy, Marian Hajduch, Petr Dzubak, Josef Srovnal, Rita Hrabakova, Hana Kovarova
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Publication number: 20140336236Abstract: Novel ALK and NTRK1 fusion molecules and uses are disclosed.Type: ApplicationFiled: April 21, 2014Publication date: November 13, 2014Applicant: FOUNDATION MEDICINE, INC.Inventors: Maureen T. Cronin, Doron Lipson, Roman Yelensky
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Publication number: 20140336070Abstract: The invention relates to improved electrochemiluminescence assay methods for phosphorylated peptides or proteins employing phospho-specific antibodies and buffer compositions that are substantially free of inorganic phosphate.Type: ApplicationFiled: June 30, 2014Publication date: November 13, 2014Inventors: Michael Tsionsky, Eli N. Glezer, Selen Stromgren, George Sigal, Jonathan K. Leland, Mark A. Billadeau, Svetlana Leytner, Mark Martin, Larry Helms
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Publication number: 20140335537Abstract: Apparatuses are disclosed for performing calorimetric assays with plucked human hair using a device adapted for that purpose. This device may include a transparent reaction vessel connected via a capillary tube to a burst pack, and connected via another capillary tube to a funnel, into which a plucked human hair may be placed.Type: ApplicationFiled: April 7, 2014Publication date: November 13, 2014Applicant: Follea International Ltd.Inventors: Andy Ofer Goren, John McCoy, Daniel Hafid
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Publication number: 20140335538Abstract: The invention relates to an epitope protection assay for use in diagnosis, prognosis and therapeutic intervention in diseases, for example, involving polypeptide aggregation, such as prion infections. The methods of the invention first block accessible polypeptide target epitope with a blocking agent. After denaturation of the polypeptide, a detecting agent is used to detect protein with target epitope that was inaccessible during contact with the blocking agent. The invention also relates to novel amyotrophic lateral sclerosis-specific epitopes and their uses to make antibodies, and to the novel antibodies and uses thereof.Type: ApplicationFiled: July 31, 2014Publication date: November 13, 2014Inventor: Neil Cashman
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Patent number: 8883144Abstract: The present invention relates to agonists of Neprilysin and their use in preventing and treating pulmonary vascular remodeling. Also described are diagnostic and screening applications stemming from the inventor's discovery that Neprilysin is expressed at reduced levels in disease tissues.Type: GrantFiled: September 1, 2011Date of Patent: November 11, 2014Assignee: The Regents of the University of Colorado, a body corporateInventor: Edward C. Dempsey
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Publication number: 20140329787Abstract: The present invention relates to a novel Phytophthora phospholipase C and uses thereof, methods of identifying modulators and inhibitors of a biological function of the phospholipase C, and methods of inhibiting Phytophthora growth comprising inhibiting a biological function of a novel Phytophthora phospholipase C.Type: ApplicationFiled: October 17, 2012Publication date: November 6, 2014Inventors: Dean Ian Phillips, Peter Luke Beech
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Publication number: 20140329706Abstract: Affinity tags and ligands comprising a PDZ domain peptide and a PDZ binding carboxy terminal peptide and related engineered protein, engineered labels, compositions, methods and systems.Type: ApplicationFiled: May 1, 2014Publication date: November 6, 2014Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGYInventor: Ward Gale WALKUP, IV
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Publication number: 20140329252Abstract: A method of determining whether a subject is at risk of developing osteoarthritis (OA), said method comprising: determining the cellular localization of a Prohibitin-1 (PHB1) polypeptide and/or Small Ubiquitin-like Modifier (SUMO) polypeptide and/or UBC9, in a cell sample from said subject; and determining whether said subject is at risk of developing OA based on the cellular localization of a PHB1 polypeptide and/or SUMO and/or UBC9 polypeptide, is described.Type: ApplicationFiled: October 15, 2012Publication date: November 6, 2014Inventor: Alain Moreau
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Publication number: 20140329253Abstract: A monoclonal antibody that binds specifically to the SNAP motif and to the CLIP tag comprising CDRs with the amino acid sequences SEQ ID Nos. 3, 4, 5, and 8, 9, 10.Type: ApplicationFiled: November 26, 2012Publication date: November 6, 2014Applicant: Fraunhofer-Gesllschaft zur Foerderung der angewandten Forschung e.V.Inventors: Stefan Barth, Katharina Kolberg, Christiane Püttmann, Severin Schmies
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Publication number: 20140329701Abstract: The invention relates to methods for deciding on the therapy in a subject suffering from colorectal cancer as well as for predicting the clinical outcome of a patient which suffers from colorectal cancer based on the expression level of KIAA1456 comprising determining the expression level of the KIAA1456 gene. The invention relates as well to kits and uses thereof comprising reagents adequate for the determination of the expression level of the KIAA1456 gene.Type: ApplicationFiled: May 11, 2012Publication date: November 6, 2014Applicant: TRASLATIONAL CANCER DRUGS PHARMA, S.L.Inventor: Fátima Valdés Mora
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Publication number: 20140322725Abstract: The present invention provides compositions comprising chimeric polypeptides that bind to free ubiquitin proteins or free ubiquitin-like proteins with high affinity, as well as chimeric polypeptides that bind to both free and conjugated ubiquitin proteins or free and conjugated ubiquitin-like proteins, and methods of using the chimeric polypeptides to determine the amount of free or total ubiquitin or free or total ubiquitin-like proteins in various types of samples.Type: ApplicationFiled: April 30, 2014Publication date: October 30, 2014Applicant: Colorado State University Research FoundationInventors: Robert E. Cohen, Yun-Seok Choi
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Publication number: 20140322699Abstract: The invention as disclosed herein provides a method for purifying a non-antibody protein from solution, comprising a chromatography step wherein the solution is passed over an affinity construct containing an affinity ligand-coupled solid support, wherein the affinity construct is associated with a bioprocess unit operation, and isolating the non-antibody protein from solution.Type: ApplicationFiled: July 8, 2014Publication date: October 30, 2014Applicant: Genzyme CorporationInventors: Veena Warikoo, Kevin Brower
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Publication number: 20140323339Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS.Type: ApplicationFiled: July 10, 2014Publication date: October 30, 2014Applicant: FLUIDIGM CANADA INC.Inventor: Olga Ornatsky
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Publication number: 20140323551Abstract: The present invention describes methods of treating cancer, cancer metastasis, and drug resistant cancers using miRNA inhibitors; for example, inhibitors of miR-409-5p. Also described are methods of using the miRNA as biomarkers; for example, to predict responsiveness to a cancer drug, to detect a disease state of cancer.Type: ApplicationFiled: November 30, 2012Publication date: October 30, 2014Applicant: CEDARS-SINAI MEDICAL CENTERInventors: Leland W.K. Chung, Sajni Josson, Murali Gururajan, Anjali Jain
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Publication number: 20140323324Abstract: The purpose of the present invention is to develop: a method for selectively separating a glycoprotein derived from the central nervous system from a body fluid or a central nervous system cell; and a method for searching for an index marker for central nervous system diseases, which utilizes the aforementioned method. A protein derived from the central nervous system, which occurs in a trace amount in a body fluid or a central nervous system cell, can be selectively enriched by a two-stage separation procedure comprising removing a glycoprotein having sialic acid at a non-reducing terminal thereof from the body fluid or the central nervous system cell and then separating a glycoprotein having N-acetylglucosamine at a non-reducing terminal thereof.Type: ApplicationFiled: December 16, 2011Publication date: October 30, 2014Applicant: National Institute of Advanced Industrial Science and TechnologyInventors: Hisashi Narimatsu, Jun Hirabayashi, Atsushi Kuno, Hideki Matsuzaki, Yuzuru Ikehara, Hiromi Ito, Yasuhiro Hashimoto, Keiro Shirotani, Satoshi Futakawa, Hajime Arai, Masakazu Miyajima, Kazuo Fujihara
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Publication number: 20140322703Abstract: A method for the quantitative determination of an analyte in a sample is provided comprising: (a) providing an analyte-specific substance which is able to undergo a reaction which generates a detectable signal when it is contacted with an analyte; (b) providing at least two calibration graphs which have been generated by reacting in each case the same analyte-specific substance with different amounts of in each case the same test analyte for in each case a predetermined reaction time; (c) contacting the analyte-specific substance with a sample which contains the analyte to be detected; (d) measuring the signal at a first predetermined reaction time for which a first calibration graph according to (b) is provided; (c) checking whether the signal measured according to (d) enables a quantitative determination of the analyte with a desired accuracy; (f) (i) quantitatively determining the analyte on the basis of the signal measured according to (d) if the desired accuracy is reached, or (ii) measuring the signal aType: ApplicationFiled: July 14, 2014Publication date: October 30, 2014Inventors: Jürgen Spinke, Marcel Thiele, Jürgen Schäffler, Andreas Nufer
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Publication number: 20140323346Abstract: The present disclosure generally relates to protein binding agents, such as protein kinase binding agents of general Formula (I). The protein binding agents may be provided attached to a solid support and may be used, for example, to detect the presence of a broad range of proteins in a sample. Methods of synthesizing the protein binding agents, and kits comprising the protein binding agents, are also disclosed.Type: ApplicationFiled: November 21, 2012Publication date: October 30, 2014Applicant: Garvan Institute of Medical ResearchInventors: Roger John Daly, Ian Peter Holmes, Ian Street, Scott Raymond Walker
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Publication number: 20140323350Abstract: A lateral test flow arrangement for a test molecule is disclosed, comprising: a test strip for transporting an analyte away from a sampling region and towards an absorbing region, the test strip having therein and remote from the sampling region, a test region for functionalization with a molecule which binds to the test molecule or to a conjugate of the test molecule; a sensing test capacitor having electrodes extending across the test strip at least partially aligned with the test region and being physically isolated therefrom; a reference test capacitor having electrodes extending across the test strip and being physically isolated therefrom; and an electronic circuit configured to measure a time-dependant capacitance difference between the sensing test capacitor and the reference test capacitor.Type: ApplicationFiled: December 6, 2012Publication date: October 30, 2014Applicant: NXP B.V.Inventors: Viet Nguyen, Franciscus Petrus Widdershoven, Roel Daamen
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Publication number: 20140322726Abstract: It is an object of the present invention to provide a monoclonal antibody, which is suitable for the quantitative analysis of asparagine synthetase in a cell. The present invention provides a monoclonal antibody which specifically recognizes asparagine synthetase that is present in a cell.Type: ApplicationFiled: October 3, 2012Publication date: October 30, 2014Inventors: Takao Hamakubo, Yasuhiro Mochizuki, Hiroko Iwanari, Osamu Arai, Toshiyuki Kitoh, Masahito Tsurusawa
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Patent number: 8871455Abstract: Methods and kits are provided for assessing radiation injury and exposure in a subject. The methods comprise measuring the levels of at least two (2) protein biomarkers from different biological pathways and correlating the levels with an assessment of radiation injury and exposure. Additional use of peripheral blood cell counts and serum enzyme biomarkers, evaluated in the early time frame after a suspected radiation exposure, and use of integrated multiple parameter triage tools to enhance radiation exposure discrimination and assessment are also provided. The information obtained from such methods can be used by a clinician to accurately assess the extent of radiation injury/exposure in the subject, and thus will provide a valuable tool for determining treatment protocols on a subject by subject basis.Type: GrantFiled: June 12, 2007Date of Patent: October 28, 2014Assignee: The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc.Inventors: William Blakely, Natalia Ossetrova, Marcy Grace, Alexandra Miller, Jean Mulimbi Muderhwa, Glen Manglapus
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Publication number: 20140315215Abstract: There is provided a single-chain fusion protein comprising: (i) a thermostable kinase and (ii) a single-domain antibody or single-domain antibody fragment. There is also provided a method of preparing a single-domain antibody or single-domain antibody fragment, the method comprising: (i) expressing the single-domain antibody or antibody fragment as a single-chain fusion protein with a thermostable kinase, in a host cell such as E. coli; and (ii) purifying the fusion protein from the cytoplasm of the host cell.Type: ApplicationFiled: September 14, 2012Publication date: October 23, 2014Applicant: The Secretary of State for HealthInventors: John Mark Sutton, Philip James Alister Skipper
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Publication number: 20140315216Abstract: An immunoassay test slide for use in a dry chemistry analytical instrument includes a slide housing or case formed from two matable sections—a slide cover piece and a slide bottom piece. The slide housing defines an interior cavity in which is situated a sheet-like porous carrier matrix. The slide cover piece has an opening formed through the thickness thereof to expose a central portion of the fluid flow matrix so that a precise volume of fluid sample of blood, serum or the like, preferably pre-mixed with a conjugate reagent, and precise volumes of a wash reagent and a substrate (detector reagent), may be deposited on the matrix through the cover opening by a metering device of the analytical instrument. The bottom piece of the immunoassay test slide is transparent, and the slide is moved by a transport mechanism of the analytical instrument over a reflectometer or a fluorometer for performing reflectance or fluorescence measurements.Type: ApplicationFiled: January 7, 2013Publication date: October 23, 2014Inventors: Eugene Chan, Keith Nassif
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Publication number: 20140315929Abstract: This invention concerns a method for selecting an inhibitor of a cancer-implicated pathway or of a component of a cancer-implicated pathway for coadministration, with an inhibitor of HSP90, to a subject suffering from a cancer which comprises the following steps: (a) contacting a sample containing cancer cells from a subject with an inhibitor of HSP90 or an analog, homolog or derivative of an inhibitor of HSP90 under conditions such that one or more cancer pathway components present in the sample bind to the HSP90 inhibitor or the analog, homolog or derivative of the HSP90 inhibitor; (b) detecting pathway components bound to the HSP90 inhibitor or to the analog, homolog or derivative of the HSP90 inhibitor; (c) analyzing the pathway components detected in step (b) so as to identify a pathway which includes the components detected in step (b) and additional components of such pathway; and (d) selecting an inhibitor of the pathway or of a pathway component identified in step (c).Type: ApplicationFiled: April 27, 2012Publication date: October 23, 2014Applicant: SLOAN-KETTERING INSTITUTE FOR CANCER RESEARCHInventor: Gabriela Chiosis
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Publication number: 20140315214Abstract: In some aspects, the invention provides methods of identifying, detecting, and/or measuring protein-protein interactions. In some aspects, the invention provides methods of identifying and/or characterizing modulators of protein-protein interactions. In some aspects, the invention provides methods of identifying and/or characterizing modulators of protein activity, wherein the methods are based at least in part on measuring interaction between a chaperone and client protein. In some aspects, the invention provides methods for identifying and/or characterizing compounds and/or for assessing compound specificity, wherein the methods are based at least in part on measuring interaction between a chaperone and client protein. In some embodiments, a client protein is a kinase. In some embodiments, a compound is a kinase inhibitor. In some aspects, the invention provides methods of profiling kinase inhibitor specificity.Type: ApplicationFiled: May 9, 2012Publication date: October 23, 2014Applicant: Whitehead Institute foe Biomedical ResearchInventors: Mikko Taipale, Susan Lindquist
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Publication number: 20140315217Abstract: A method for assaying activity of the enzyme spermidine/spermine N1-acetyltransferase (SSAT) uses SSAT substrates by detecting their acetylated forms. SSAT substrates may include rimantadine and tocainide wherein their metabolism occurs in part by the action of the inducible enzyme SSAT to produce the acetylated metabolites N-acetylrimantadine and N-acetyltocainide respectively. SSAT activity may be correlated to pathologic conditions.Type: ApplicationFiled: November 16, 2012Publication date: October 23, 2014Applicant: BioMark Technologies Inc.Inventors: Brian Cheng, Rashid Bux, Daniel Sitar
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Publication number: 20140315752Abstract: The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using a one or more assays configured to detect a kidney injury marker selected from the group consisting of Stanniocalcin-1, Antithrombin-III, Toll-like receptor 2, Triiodothyronine (T3), Thyroxine (T4), Extracellular matrix protein 1, Coagulation factor XIII A chain, Coagulation factor XIII B chain, Interleukin-17F, Interleukin-22, Vitronectin, Progesterone, Estradiol, Growth/differentiation factor 15, and Proprotein convertase subtilisin/kexin type 9 as diagnostic and prognostic biomarkers in renal injuries.Type: ApplicationFiled: November 20, 2012Publication date: October 23, 2014Inventors: Joseph Anderberg, Jeff Gray, Paul McPherson, Kevin Nakamura, James Patrick Kampf
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Patent number: 8865420Abstract: The present invention provides an approach for the simultaneous determination of the activation states of a plurality of proteins in single cells. This approach permits the rapid detection of heterogeneity in a complex cell population based on activation states, and the identification of cellular subsets that exhibit correlated changes in activation within the cell population. Moreover, this approach allows the correlation of cellular activities or properties. In addition, the use of potentiators of cellular activation allows for characterization of such pathways and cell populations.Type: GrantFiled: May 22, 2008Date of Patent: October 21, 2014Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Omar D. Perez, Garry P. Nolan, Jonathan M. Irish
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Publication number: 20140308679Abstract: The invention relates to fluorometry, and in particular to methods and apparatus for time-delayed detection of fluorescence in a sample, for example for use in a clinical setting. Exemplary embodiments disclosed relate to a fluorometer (100) comprising a light source (101), a light detector (102), a sample holder (103) disposed between the light source and light detector, a motor (104); and a light transmission modulator (105) disposed around the sample holder and comprising a pair of plates (106, 107) attached to the motor for rotation about a common axis (108), the pair of plates arranged to allow transmission from the source to the sample in a first rotated position, to allow transmission from the sample to the detector in a second rotated position and to block direct light transmission between the source and detector in both first and second rotated positions.Type: ApplicationFiled: June 16, 2014Publication date: October 16, 2014Inventors: Edward John Thompson, Ilan Tal
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Publication number: 20140309141Abstract: Methods and compositions for detection of the modulators of proteolytic enzymes, particularly cysteine proteases, are disclosed.Type: ApplicationFiled: September 21, 2012Publication date: October 16, 2014Inventors: Craig Leach, James Strickler, Michael Eddins
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Publication number: 20140308680Abstract: The present invention relates to a method for simultaneous detection of the coagulation activity of a blood sample and of the presence and/or amount of at least one target molecule within said blood sample, comprising the steps of: i) introducing a blood sample into a sample container comprising magnetic particles and a sensor surface, wherein said magnetic particles are functionalized with a first binding molecule, wherein said first binding molecule is attached to said magnetic particles, wherein the first binding molecule is capable of specifically binding to the at least one target molecule within said blood sample, and wherein a second binding molecule is attached to a sensor surface at the bottom of said sample container, and wherein said second binding molecule of the sensor surface is capable of specifically binding to the at least one target molecule within said blood sample; ii) measuring the presence and/or amount of said target molecule by detecting the number of magnetic particles bound to said sType: ApplicationFiled: October 3, 2012Publication date: October 16, 2014Inventor: Ron Martinus Laurentius Van Lieshout
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Publication number: 20140309171Abstract: The invention relates to the use of phosphorylated Acetyl-CoA carboxylase (ACC) in platelets from a subject as a biomarker for blood coagulation, preferably blood coagulation in the subject, preferably encompassing activation of the coagulation cascade as well as platelet activation, preferably thrombin-induced platelet activation. The invention encompasses uses and methods for the diagnosis or the monitoring of blood coagulation in the subject. The invention further relates to uses and methods for screening for anti-coagulants or pro-coagulants.Type: ApplicationFiled: November 21, 2012Publication date: October 16, 2014Inventors: Sandrine Horman, Christophe Beauloye, Marie-Blanche Onselaer, Luc Bertrand, Jean-Louis Vanoverschelde
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Publication number: 20140308242Abstract: The invention relates to biomarkers measurable in a human subject that have prognostic value with respect to efficacy of therapeutic treatments for Hepatitis C viral infection. The markers also are believed to have value for diagnosis liver health/liver damage.Type: ApplicationFiled: October 21, 2011Publication date: October 16, 2014Applicant: VERTEX PHARMACEUTICALS INCORPORATEDInventors: Mark Andrew Fleming, Brain J. Hare, Daniel Chelsky, Rene Allard, Eustache Paramithiotis
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Publication number: 20140309263Abstract: The present invention relates to methods of diagnosing a kidney disorder in a patient, as well as methods of monitoring the progression of a kidney disorder and/or methods of monitoring a treatment protocol of a therapeutic agent or a therapeutic regimen. The invention also relates to assay methods used in connection with the diagnostic methods described herein.Type: ApplicationFiled: January 31, 2014Publication date: October 16, 2014Applicant: MESO SCALE TECHNOLOGIES, LLCInventors: Simone Barbero, Eli N. Glezer, Anu Mathew, Mingyue Wang
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Publication number: 20140309135Abstract: A method of screening a compound having a hypoglycemic effect (hereinafter referred to as “hypoglycemic compound”), a remedy for diabetes which contains a compound having a novel function mechanism, etc. More specifically speaking, a method of screening a hypoglycemic compound capable of binding to the ? subunit of a trimeric GTP-binding protein, a remedy for diabetes comprising a hypoglycemic compound, which is characterized by being capable of binding to the ? subunit of a trimeric GTP-binding protein, as the active ingredient, etc.Type: ApplicationFiled: April 25, 2014Publication date: October 16, 2014Applicant: AJINOMOTO CO., INC.Inventors: Naoyuki FUKUCHI, Satoru Okamoto, Wataru Miyanaga, Sen Takeshita, Masaru Takayanagi, Yumiko Fukuda, Takao Ikenoue, Naoyuki Yamada, Naoko Arashida
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Patent number: 8859223Abstract: Molecular probes are provided for use in fluorescence microscopy procedures for monitoring beta-secretase enzyme (BACE) activity in living cells and organisms. The probes may be useful for monitoring Alzheimer's Disease-associated BACE in living cells. By fluorescing when hydrolyzed by BACE, the probes can allow for real-time spatial and temporal assessment of enzymatic activity without the need for mutated cell lines or antibodies. The molecular probes may also be used to screen libraries of potential BACE inhibitors or evaluate how external stimuli affect BACE activity.Type: GrantFiled: March 15, 2013Date of Patent: October 14, 2014Assignee: Duke UniversityInventors: Katherine J. Franz, Drew Folk
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Publication number: 20140301945Abstract: Provided are novel human copper-zinc superoxide dismutase, also known as superoxide dismutase 1 or SOD1, specific antibodies as well as fragments, derivatives and variants thereof as well as methods related thereto. Assays, kits, and solid supports related to antibodies specific for SOD1 are also disclosed. The antibody, immunoglobulin chain(s), as well as binding fragments, derivatives and variants thereof can be used in pharmaceutical and diagnostic compositions for SOD1 targeted immunotherapy and diagnosis, respectively.Type: ApplicationFiled: December 19, 2011Publication date: October 9, 2014Applicants: UNIVERSITY OF ZURICH, NEURIMMUNE HOLDING AGInventors: Fabio Montrasio, Maria Grazia Barenco Montrasio, Jan Grimm, Roger Nitsch, Christoph Hock, Tobias Welt, Jordan McAfoose, Marcel Maier
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Publication number: 20140302047Abstract: This invention relates to isolated antibodies which recognise the exosite 1 epitope of thrombin and selectively inhibit thrombin without promoting bleeding. These antibody molecules may be useful in the treatment and prevention of thrombosis, embolism and other conditions mediated by thrombin.Type: ApplicationFiled: June 19, 2014Publication date: October 9, 2014Inventors: James Andrew Huntington, Trevor Baglin, Jonathan Langdown
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Publication number: 20140302525Abstract: Provided herein are methods of detecting binding of an SENP1 polypeptide to a compound and methods for screening for inhibitors of SENP1. Further provided are aqueous compositions comprising SENP1 polypeptides and NMR apparatuses comprising the compositions for NMR analysis.Type: ApplicationFiled: April 7, 2014Publication date: October 9, 2014Applicant: City of HopeInventor: Yuan Chen
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Publication number: 20140303018Abstract: Methods, compositions, and kits for determining whether a subject has non-alcoholic fatty liver disease (NAFLD) are provided. Methods, compositions, and kits for determining whether a subject has non-alcoholic steatosis are also provided. Methods, compositions, and kits for determining whether a subject has non-alcoholic steatohepatitis (NASH) are also provided.Type: ApplicationFiled: March 10, 2014Publication date: October 9, 2014Applicant: SomaLogic, Inc.Inventors: Malti Nikrad, Stuart G. Field, Stephen Alaric Williams
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Publication number: 20140303191Abstract: Methods are provided for treating a hematologic cancer comprising administering an anticancer agent to a subject identified as having an increased mobilization of a subpopulation of lymphocytes from a malignancy following administration of an irreversible Btk inhibitor. Methods also are provided for identification of subjects for treatment and the analysis of cells mobilized from a hematologic malignancy following administration of an irreversible Btk inhibitor.Type: ApplicationFiled: October 19, 2012Publication date: October 9, 2014Inventors: Joseph J. Buggy, Laurence Elias, Gwen Fyfe, Eric Hedrick, David J. Loury, Tarak D. Mody
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Publication number: 20140302998Abstract: The present application provides stable peptide-based Akt capture agents and the use thereof as detection, diagnosis, and treatment agents. The application further provides novel methods of developing stable peptide-based capture agents, including Akt capture agents, using iterative on-bead in situ click chemistry.Type: ApplicationFiled: July 11, 2012Publication date: October 9, 2014Inventors: James R. Heath, Arundhati Nag, Samir Das, Kaycie M. Deyle, Steven Wesley Millward, Paul Edward Kearney