To Identify An Enzyme Or Isoenzyme Patents (Class 435/7.4)
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Publication number: 20140206017Abstract: The present disclosure provides an assay to detect and/or quantify circulating lysyl oxidase-like 2 (LOXL2) poly-peptides in an individual. The assay is useful in diagnostic and prognostic applications, which are also provided.Type: ApplicationFiled: June 1, 2012Publication date: July 24, 2014Inventors: Victoria Smith, Joanne I. Adamkewicz, Susan K. Lyman, Jason Chien, Xiaoming Li, Lixin Shao, Jeffrey D. Bornstein
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Publication number: 20140205592Abstract: The present invention relates to a method for determining whether a cancer patient is susceptible to treatment with a protein tyrosine kinase 2 (PTK2) inhibitor, comprising detecting the expression of the E-cadherin protein in a cancer sample of said cancer patient, wherein an E-cadherin protein immunoreactivity score (IRS) of 0-2 indicates that the cancer patient is susceptible to treatment with a PTK2 inhibitor. Said detection of the expression of the E-cadherin protein in a cancer sample of a cancer patient is preferably conducted by way of an immunohistochemistry (IHC) method. Said IHC method preferably employs a primary antibody which is specific for E-cadherin and a secondary antibody which specifically reacts with the primary antibody. The present invention also relates to a method of treating a cancer patient whose cancer is characterized by an E-cadherin protein immunoreactivity score (IRS) of 0-2, comprising administering to the patient a therapeutically effective amount of a PTK2 inhibitor.Type: ApplicationFiled: March 21, 2014Publication date: July 24, 2014Applicant: Boehringer Ingelheim International GmbHInventors: Guenther ADOLF, Pilar GARIN-CHESA, Ulrich HIRT
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Patent number: 8785142Abstract: The invention relates to an arrangement, comprising a solid carrier and a matrix arranged on the solid carrier, said matrix comprising at least one enzymatically convertible or modifiable molecule and comprising at least one enzyme that can be released by the conversion or modification of the molecule, said enzyme being capable of converting at least one color-changing substrate located in the matrix and/or on the solid carrier.Type: GrantFiled: February 9, 2011Date of Patent: July 22, 2014Inventors: Georg Gübitz, Eva Sigl, Andrea Hasmann, Marc Schröder, Konstantin Schneider, Alexandra Rollett, Franz Kaufmann, Andreas Hafner
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Publication number: 20140199706Abstract: This invention relates to methods and compositions for enhancing proteasome activity in a cell. The methods and compositions for enhancing the activity of the proteasome in cells modulate the activity of Ubp6 (yeast) or Usp14 (human), an endogenous inhibitor of the proteasome. The methods and compositions partially or completely reduce the inhibitory activity of Usp14 on a proteasome, thereby specifically enhancing the protein-degradation activity of the proteasome. The invention also provides methods of screening to identify inhibitors of Ubp6, Usp14, and/or both Ubp6 and Usp14.Type: ApplicationFiled: September 10, 2013Publication date: July 17, 2014Applicant: President and Fellows of Harvard CollegeInventors: Daniel J. Finley, John W. Hanna, Nathaniel A. Hathaway, Randall W. King, Byung-Hoon Lee
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Publication number: 20140199324Abstract: Provided are compositions, methods and kits for quantifying the expression and/or activity of MMP-14 and other biomarkers of cancer, which may be used diagnostically and prognostically, e.g., in patient stratification and evaluation of appropriate therapeutic regimens.Type: ApplicationFiled: March 23, 2012Publication date: July 17, 2014Inventor: Daniel T. Dransfield
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Publication number: 20140199709Abstract: The invention provides an ELISA assay for the determination of serum mast cell ?-tryptase levels using rabbit anti-tryptase as the capture antibody and alkaline phosphatase conjugated G3 as the detecting antibody. Luminescent substrate CPSD was used to enhance the assay sensitivity. Also provided are methods for aiding in the diagnosis of irritable bowel syndrome by detecting the serum level of ?-tryptase, histamine and/or prostaglandin E2.Type: ApplicationFiled: March 21, 2014Publication date: July 17, 2014Applicant: Nestec S.A.Inventors: HUA GONG, Shui Long Wang, Sharat Singh
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Publication number: 20140199707Abstract: Provided are methods for identifying and/or designing inhibitors of Staphylococcus aureus biological activity. Also provided is a computer readable medium encoded with instructions executable by a CPU for performing the functions of identifying or designing inhibitors of Staphylococcus aureus biological activity, and a system comprising the computer readable medium and a CPU. Also provided are compounds identified and/or designed by the methods, methods of treatment including the compounds, methods of inhibiting S. aureus using the compounds, medicaments including the compounds for use in the treatment of S. aureus infection, or conditions related to S. aureus infection, and pharmaceutical compositions including the compounds. Methods for identifying a bacterial cell that is resistant to methicillin are also provided.Type: ApplicationFiled: December 13, 2013Publication date: July 17, 2014Applicant: Indian Institute of ScienceInventors: Girish S. Tavarekere, Gopal Balasubramanian
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Publication number: 20140199705Abstract: The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.Type: ApplicationFiled: September 5, 2013Publication date: July 17, 2014Applicants: SYNGENTA PARTICIPATIONS AG, BP CORPORATION NORTH AMERICA INC.Inventors: Brian Steer, Walter Niles Callen, Shaun Healey, Derrick Pulliam
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Publication number: 20140199708Abstract: The invention provides a method for determining the activity of an inhibitor of a target protein that has a deleterious effect on cell growth. The principle of the claimed method is that a compound that inhibits the function of an overexpressed protein will relieve the detrimental effect of such overexpression in a concentration-dependent manner, thereby allowing the determination of the activity of the compound in inhibiting its target protein in live cells.Type: ApplicationFiled: January 13, 2014Publication date: July 17, 2014Applicant: SENEX BIOTECHNOLOGY INC.Inventors: Igor B. Roninson, Donald C. Porter, Serena Altilia
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Patent number: 8778335Abstract: A method for treating a Parkinson's patient with digestive/pancreatic enzymes involves administering an effective amount of digestive/pancreatic enzymes to an individual having the disorder in order to improve a symptom of the disorder. In addition, a method is provided for determining whether an individual has, or may develop, Parkinson's disease or related dysautonomic disorders and for determining whether an individual will benefit from the administration of pancreatic/digestive enzymes to treat the dysautonomic disorder.Type: GrantFiled: March 11, 2008Date of Patent: July 15, 2014Assignee: Curemark, LLCInventor: Joan M. Fallon
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Patent number: 8778622Abstract: Disclosed herein are methods and kits which are useful for detecting presence of an enzyme in a test sample based upon the intrinsic enzymatic activity of such test sample. The present invention provides the ability to evaluate cell culture conditions and optimize the desired glycoform content of recombinantly prepared enzymes.Type: GrantFiled: November 30, 2010Date of Patent: July 15, 2014Assignee: Shire Human Genetic Therapies, Inc.Inventor: Daniel S. Roseman
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Patent number: 8778623Abstract: Differentiated cholinergic cells having motor neuron-like morphology and increased sensitivity to botulinum neurotoxin are provided herein. Methods of using such differentiated cells for detecting neurotoxin are also provided.Type: GrantFiled: February 28, 2013Date of Patent: July 15, 2014Assignee: Wisconsin Alumni Research FoundationInventors: Eric Arthur Johnson, Regina Whitemarsh, Sabine Pellett
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Publication number: 20140196166Abstract: An isolated nucleic acid molecule that encodes a terpene synthase and is selected from among: a) a nucleic acid molecule comprising the sequence of nucleotides set forth in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5; b) a nucleic acid molecule that is a fragment of (a); c) a nucleic acid molecule comprising a sequence of nucleotides that is complementary to (a) or (b); and d) a nucleic acid molecule that encodes a terpene synthase having at least or at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% identity to any one of (a)-(c); wherein the nucleic acid molecule encodes a terpene synthase.Type: ApplicationFiled: September 24, 2013Publication date: July 10, 2014Inventors: Katherine Zulak, Christopher Jones, Jessie Moniodis, Joerg Bohlmann
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Publication number: 20140194325Abstract: The present invention provides compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, provided herein are bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith.Type: ApplicationFiled: December 12, 2013Publication date: July 10, 2014Applicant: Promega CorporationInventors: Carolyn W. Hitko, Thomas Kirkland, Thomas Machleidt, Rachel Friedman Ohana, Matt Robers, Keith Wood
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Publication number: 20140194307Abstract: The present invention provides compositions and methods for detection and analysis of intracellular binding of a bioactive agent to a cellular target. In particular, provided herein are bioactive agents tethered to fluorophores, cellular targets fused to bioluminescent reporters, or portions, components, or subunits of bioluminescent reporters, and methods of detecting and analyzing the interaction of bioactive agents with cellular targets therewith.Type: ApplicationFiled: December 12, 2013Publication date: July 10, 2014Applicant: Promega CorporationInventors: Carolyn W. Hitko, Thomas Kirkland, Thomas Machleidt, Rachel Friedman Ohana, Matt Robers, Keith Wood
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Publication number: 20140193833Abstract: Provided are methods of using polycarbonate filters to isolate and detect cancer cells in a biological fluid, particularly biological fluids, such as urine, that contain very low concentrations of cancer cells. The characterization of the isolated cells for the presence or absence of cancer specific proteins is useful for cancer diagnosis and prognosis.Type: ApplicationFiled: August 8, 2012Publication date: July 10, 2014Applicant: THE HENRY M. JACKSON FOUNDATION FOR THE ADVANCEMENT OF MILITARY MEDICINE, INC.Inventors: Shiv K. Srivastava, Petrovics Gyorgy, Shyh-Han Tan, Kristen Nickens
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Publication number: 20140194317Abstract: A method of detecting the presence of specific human papilloma virus and host cell biomarkers associated with head and neck tumors in biological samples, like saliva, blood or biopsy tissue, obtained from a subject.Type: ApplicationFiled: January 23, 2014Publication date: July 10, 2014Applicant: LOMA LINDA UNIVERSITYInventors: Penelope J. Duerksen-Hughes, Maria Filippova, Valeri Filippov
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Publication number: 20140193832Abstract: The present invention features novel methods for determining if a subject has prostate cancer. The present invention is based on the development of lectin immunosorbant assays which analyze ?2,6-linked sialylation of total serum PSA by sambucus nigra lectin (SNA) and ?2,3-linked sialylation of total and free serum PSA. These novel assays were used then to conduct a clinical investigation of the potential role of glycoprotein analysis in improving PSA's cancer specificity. The present invention also features kits for determining if a subject has prostate cancer comprising one or more lectins and a PSA specific antibody and instructions for use.Type: ApplicationFiled: November 26, 2013Publication date: July 10, 2014Applicant: THE JOHNS HOPKINS UNIVERSITYInventors: Hui Zhang, Danni Li Meany, Daniel W. Chan, Zhen Zhang, Yan Li, Mori J. Sokoll
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Publication number: 20140193373Abstract: In some embodiments, methods for improving the survival of pancreatic ?-cell progenitors in culture are provided. Such methods may include contacting a population of pancreatic progenitor cells with an amino acid (aa) sequence comprising IKVAV (SEQ ID NO:1). In other embodiments, methods for (i) verifying the establishment of a population of pancreatic progenitor or stem cells and (ii) methods for generating or establishing a population of pancreatic endocrine progenitor cells in vitro are provided.Type: ApplicationFiled: March 14, 2013Publication date: July 10, 2014Inventor: Hsun Ku
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Publication number: 20140193369Abstract: According to some embodiments, a method selects one or more inflammatory markers to evaluate. The one or more inflammatory markers selected from the group consisting of iNOS, COX-2, TNF-alpha, NO, PGE2, IL-6, and IL-1?. The method determines a first expression level and a second expression level of the one or more inflammatory markers. The first expression level is associated with a positive control sample comprising a type of human macrophages. The second expression level is associated with a test sample obtained by exposing a food product to a digest, the type of human macrophages, and a pro-inflammatory compound. The method performs a comparison between the first expression level and the second expression level and designates the food product according to an anti-inflammatory standard based on the comparison.Type: ApplicationFiled: January 7, 2013Publication date: July 10, 2014Applicant: WhiteWave Services, Inc.Inventor: Neal Allan Bringe
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Publication number: 20140193831Abstract: The present invention relates to a method of predicting a plant seed trait, such as germination rate, vigour, aging and priming, by determining the presence of a target protein, i.e. diagnostic marker, in its active state in a protein sample derived from a plant seed or plant seed lot. Thereby, the quality of a plant seed or a plant seed lot can be predicted and/or diagnosed and seeds may be distinguished on basis of their characteristics and with respect to the traits described herein. Inter alia, seeds and seed lots having high germination quality may be distinguished from seeds and seed lots having low germination quality.Type: ApplicationFiled: January 30, 2012Publication date: July 10, 2014Applicants: GAUTIER SEMENCES, NINSAR AGROSCIENCES SLInventors: Renier A. L. Van Der Hoorn, Christian Gu, Kaschani Farnusch, Johana Misas, Kerstin Richau, Izabella Kolodziejek, Oscar Goni Ramos, Xavier Berthet, Philippe Ellul, Frédéric Moquet, Capucine Malliart
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Publication number: 20140194314Abstract: Multi-color CL images of nanoparticle samples may be generated, by irradiating with a scanning electron beam a nanoparticle sample that containing a plurality of spectrally distinct optical emitters configured to generate CL light at respective different color channels, then detecting the CL light from the nanoparticles to generate multi-color NP-CL images of the nanoparticle sample. In some embodiments, SE (secondary electron) images of the sample may be acquire, substantially simultaneously with the acquisition of the CL images, so as to generate correlative NP-CL and SE images of the nanoparticle sample. In some embodiments, the nanoparticles may be surface-functionalized so that the nanoparticles selectively bind only to particular structures of interest.Type: ApplicationFiled: June 13, 2012Publication date: July 10, 2014Applicant: President And Fellows of Harvard CollegeInventors: Ronald Walsworth, Jeff Lichtman, Narayanan Kasthuri, David Glenn, Huiliang Zhang, Richard Schalek
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Publication number: 20140186852Abstract: This invention relates, e.g., to a method for determining if a subject has myocardial ischemia, comprising (a) providing a blood sample obtained from a subject suspected of having myocardial ischemia; (b) determining in the sample the amount of one or more of the following proteins: (i) Lumican and/or (ii) Extracellular matrix protein 1 and/or (iii) Carboxypeptidase N; and (c) comparing the amount(s) of the protein(s) to a baseline value that is indicative of the amount of the protein in a subject that does not have myocardial ischemia, wherein a statistically significantly increased amount of the protein(s) compared to the baseline value is indicative of myocardial ischemia. Other proteins indicative of myocardial ischemia are also described, as are methods for treating a subject based on a diagnostic procedure of the invention, and kits for carrying out a method of the invention.Type: ApplicationFiled: July 29, 2013Publication date: July 3, 2014Applicant: The Johns Hopkins UniversityInventors: Jennifer Van Eyk, Simon Sheng, Qin Fu
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Publication number: 20140186855Abstract: Methods, assays, and apparatus are disclosed for testing of antigens associated with intestinal and/or blood-brain barrier permeability. For example, blood, saliva or other bodily fluid can be tested for binding (1) to a bacterial toxin (preferably a lipopolysaccharide), and (2) binding to tissue antigens selected from at least one of (a) a gut-related antigen and (b) a blood brain barrier-related antigen. Analysis of test results can be used to assist in detecting and diagnosing diseases associated with leaky gut syndrome (whether due to paracellular or transcellular pathways, and whether due to bacterial toxins or some other cause) and/or to diseases associated with excessive blood brain barrier permeability, which are contemplated herein to include both neuroinflammation and/or neuroautoimmunity conditions, and especially amyotrophic lateral sclerosis, Parkinson's disease, multiple sclerosis, Alzheimer's, or peripheral neuropathy, and major depression.Type: ApplicationFiled: March 4, 2014Publication date: July 3, 2014Applicants: Immunosciences Lab, Inc., Cyrex Laboratories, LLCInventor: Aristo Vojdani
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Publication number: 20140189893Abstract: Methods of identifying compounds that specifically modulate the interaction of FGFR1 and ?-Klotho are disclosed. Identified compounds can be useful in treating metabolic diseases and disorders that involve the interaction of FGFR1 and ?-Klotho. In various embodiments the metabolic disease or disorder is diabetes, obesity, dyslipidemia, elevated glucose levels, elevated insulin levels and diabetic nephropathy.Type: ApplicationFiled: February 20, 2012Publication date: July 3, 2014Applicant: AMGEN INC.Inventors: Yang Li, Xinle Wu, Hongfei Ge, Helene Baribault, Bryan Lemon, Jackie Z. Sheng, Steven Vonderfecht, Jennifer Veronica Weiszmann, Jonitha Gardner, Grace Ki Jeong Lee
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Publication number: 20140178884Abstract: Disclosed herein are methods of diagnosing, monitoring and treating an Aspergillus species-associated condition, such as Aspergillus fumigatus (Af)-associated condition, including aspergillosis, such as invasive aspergillosis (IA). Methods for diagnosing and/or monitoring an Aspergillus species-associated condition, such as IA are provided. Also disclosed are point-of-care immunoassays that can be used to diagnose or monitor the efficacy of an Aspergillus-associated condition treatment. These immunoassays can also be used for rapid diagnosis of infection produced by an Aspergillus species, such as Af.Type: ApplicationFiled: May 22, 2012Publication date: June 26, 2014Applicant: BOARD OF REGENTS OF THE NEVADA SYSTEM OF HIGHER EDUCATION, ON BEHALF OF THE UNIV. OF NEVADA, RENOInventors: David Aucoin, Thomas R. Kozel, Sindy Chaves
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Publication number: 20140178887Abstract: The methods and apparatus of the present invention allow the evaluation of inflammation of the esophagus. Measurements may be utilized, for example, to diagnose a disease of the esophagus, to monitor inflammation of the esophagus, or to access the treatment of a disease of the esophagus. In one embodiment, the invention comprises a method for measuring esophageal inflammation comprising deploying a device into the esophagus of a subject, removing the device after a predetermined period of time, analyzing the device for a diagnostic indicator of esophageal inflammation and evaluating the diagnostic indicator to diagnose esophageal inflammation.Type: ApplicationFiled: December 20, 2013Publication date: June 26, 2014Applicants: The Board Of Trustees Of The University Of Illinois, The Regents Of The University Of Colorado, A Body CorporateInventors: Glenn T. FURUTA, Steven J. ACKERMAN
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Publication number: 20140178393Abstract: Herein is reported a fusion polypeptide according to formula (I): NH2—S2—X1—S1—COOH, wherein X1 comprises either a random amino acid sequence or an amino acid sequence derived from a first polypeptide, S2 and S1 are non-overlapping amino acid sequences derived from a second polypeptide, and denotes a peptide bond, wherein the second polypeptide is a polypeptide with peptidyl-prolyl cis/trans-isomerase activity (PPIase activity) or is derived from the FKBP-fold domain family, wherein X1 is inserted in place of the insert-in-flap-domain of the second polypeptide.Type: ApplicationFiled: May 4, 2012Publication date: June 26, 2014Inventors: Herbert Andres, David Casagolda Vallribera, Hartmut Duefel, Michael Gerg, Christian Scholz, Michael Schraeml
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Publication number: 20140178893Abstract: Methods and kits for diagnosing the presence of and prognosing the appearance of tissue remodelling-associated conditions, involving the presence of enzymes in a biological sample, are disclosed. In particular, the method pertains to diagnosing the presence of or prognosing appearance of cancer, metastatic cancer, and obstructive and degenerative conditions.Type: ApplicationFiled: July 16, 2013Publication date: June 26, 2014Inventors: Marsha A. Moses, Michael R. Freeman, Dmitri Wiederschain
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Publication number: 20140179620Abstract: The present invention relates to methods of gene expression profiling for inflammatory bowel disease pathogenesis, in which the differential expression in a test sample from a mammalian subject of one or more IBD markers relative to a control is determined, wherein the differential expression in the test sample is indicative of an IBD in the mammalian subject from which the test sample was obtained.Type: ApplicationFiled: August 12, 2013Publication date: June 26, 2014Applicant: GENENTECH, INC.Inventors: Alexander R. Abbas, Hilary Clark, Lauri Diehl, Charles Lees, Colin L. Noble, Jack Satsangi
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Publication number: 20140178894Abstract: The present application relates to a composition comprising the components listed in Table I in an amount that will lead to the respective concentration specified in Table I for each of said component when said composition is diluted with water into 1× medium, said composition being particularly suitable to generate a chemically-defined medium suitable for culturing cells, especially primary cells, maintaining their proliferation and differentiation potential.Type: ApplicationFiled: April 20, 2011Publication date: June 26, 2014Applicant: NOVARTIS FORSCHUNGSSTIFTUNG, ZWEIGNIEDERLASSUNGInventors: Mohamed Bentires-Alj, Stephan Duss
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Publication number: 20140178339Abstract: Methods of treating a tumor in a subject include identifying a subject having, at risk for, or suspected of having a tumor, and administering to the subject an effective amount of an IGFBP7 agent if the tumor has increased Ras-BRAF-MEK-Erk signaling, is dependent for growth and/or survival upon the Ras-BRAF-MEK-Erk signaling pathway, and/or expresses an activated or oncogenic BRAF or RAS.Type: ApplicationFiled: December 2, 2013Publication date: June 26, 2014Inventors: Michael Green, Narendra Wajapeyee
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Publication number: 20140178366Abstract: The present invention provides methods for the preselection of a subject for therapeutic treatment with an agent based on modulated levels of hypoxia in cancerous cells in the subject. In one embodiment, the invention provides methods for the preselection of a subject for therapeutic treatment with an agent based on modulated levels of lactate dehydrogenase (LDH) in a cell, e.g., a cancerous cell. The invention also provides methods for treating cancer in a subject by administering an effective amount of an agent to the subject, wherein the subject has been selected based on a modulated level of hypoxia. The invention further provides kits to practice the methods of the invention.Type: ApplicationFiled: December 17, 2013Publication date: June 26, 2014Applicant: SYNTA PHARMACEUTICALS CORP.Inventors: Ronald K. Blackman, Vojo Vukovic
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Patent number: 8759012Abstract: The object of the present invention is to provide a method of determining the dose and/or administration of statins to a patient suffering from a cardiovascular disease. The object is achieved by the method of determining the dose and/or administration of statins to a patient suffering from a cardiovascular disease comprising Step (1) of measuring the intracellular SmgGDS expression level of a patient suffering from a cardiovascular disease before and after administration of statin; and Step (2) of determining the type and/or the dose of statin for the patient in reference to the SmgGDS expression level measured in the Step (1).Type: GrantFiled: December 9, 2011Date of Patent: June 24, 2014Assignee: Tohoku UniversityInventors: Hiroaki Shimokawa, Shin-ichi Tanaka
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Patent number: 8759018Abstract: A method for determining an appropriate treatment option for a patient who has been diagnosed with disseminated intravascular coagulation (DIC) but who may have thrombotic thrombocytopenic purpura (TTP), by analyzing the amount and/or enzyme activity of a von Willebrand factor (vWF)-cleaving protease (ADAMTS13) and the amount of vWF in a patient that has been diagnosed with DIC is disclosed. Using the method of the present invention, a differential diagnosis of patients with thrombotic thrombocytopenic purpura (TTP) can be made from among patients diagnosed with DIC, which could not previously be distinguished on the basis of only clinical findings or known markers. Also disclosed is a kit for determining an appropriate treatment option, the kit comprising an antibody or a fragment thereof which specifically binds to ADAMTS13.Type: GrantFiled: February 18, 2011Date of Patent: June 24, 2014Assignees: Mitsubishi Chemical Medience Corporation, Juridical Foundation the Chemo-Sero-Therapeutic Research InstituteInventors: Tomoko Ono, Shinichiro Watanabe, Fumio Furusaki, Ko Igami
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Publication number: 20140170674Abstract: This invention discloses an immunochromatographic assay device to detect and quantify analytes. The device utilizes up-converting luminescent probes to detect time-resolved luminescence signals. Because the unconverting luminescent probes can have relatively long emission lifetime, background interference from sample autofluorescence and light scattering from excitation source can be easily eliminated through delayed luminescence detection. Furthermore, the up-converting luminescent probes can be excited by near Infrared (IR) or IR light sources. In comparing with UV and visible lights, the near IR and IR lights can penetrate deeper into sample matrices and more effectively excite the probes, but not the sample matrices, resulting in less background and higher detection sensitivity. A simple and low cost reader can be designed to measure the delayed up-converting luminescence of long lifetime that does not use expensive optical filters and mirrors.Type: ApplicationFiled: December 28, 2012Publication date: June 19, 2014Inventor: Aimin He
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Publication number: 20140170659Abstract: An in vitro method for detecting the susceptibility of a tumor cell to a chemotherapy is disclosed. The method includes the step of measuring the expression level of the isoform 202 of the ERCC1 protein.Type: ApplicationFiled: February 21, 2014Publication date: June 19, 2014Applicant: INSTITUTE GUSTAVE-ROUSSYInventors: Ken Olaussen, Jean-Charles Soria, Luc Friboulet
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Publication number: 20140171373Abstract: Disclosed herein are isolated polypeptides, antibody preparations, treatment methods, diagnostic methods, and screening methods related to tauopathy. Generally, the isolated polypeptide includes a core pentapeptide, with the proviso that the isolated polypeptide is not a native full-length tau protein. Generally, the antibody preparations include antibody that specifically binds to SEQ ID NO:12. Generally, the treatment methods include administering to a subject a composition that includes the isolated polypeptide. Generally, the diagnostic methods includes contacting a sample from a subject with an antibody preparation that includes antibody that specifically binds to SEQ ID NO:12, and then detecting a ligand in the sample that specifically binds the antibody preparation.Type: ApplicationFiled: December 18, 2013Publication date: June 19, 2014Applicant: Regents of the University of MinnesotaInventors: Karen Hsiao Ashe, Xiaohui Zhao, Michael Anthony Walters, Derek John Hook, Morgan Clotaire Paul Le Naour
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Publication number: 20140171439Abstract: This invention comprises the novel compounds of formula (I) wherein n, R1, R2, R3, R4, Q, X, Y, Z and have defined meanings, having histone deacetylase inhibiting enzymatic activity; their preparation, compositions containing them and their use as a medicine.Type: ApplicationFiled: February 21, 2014Publication date: June 19, 2014Applicant: Janssen Pharmaceutica, N.V.Inventors: Kristof VAN EMELEN, Marc Gustaaf Celine VERDONCK, Sven Franciscus Anna VAN BRANDT, Patrick Rene ANGIBAUD, Lieven MEERPOEL, Alexey Borisovich DYATKIN
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Publication number: 20140170675Abstract: The invention provides a semiconductor device for the detection of an active site-containing protein or a ligand thereof in a solution, said device comprising at least one insulating or semi-insulating layer; at least one conducting semiconductor layer, two conducting pads on top of the upper layer making electrical contact with said at least one conducting semi-conductor layer, such that electrical current can flow between them at a finite distance from the surface of the device; a protective molecular layer fabricated on top of said upper layer and protecting said layer from corrosion; and said ligand or active site-containing protein linked to said protective molecular layer. Exposure of said ligand or active site-containing protein to a solution containing said active site-containing protein or ligand, respectively, causes a current change through the device when a constant electric potential is applied between the two conducting pads.Type: ApplicationFiled: May 31, 2012Publication date: June 19, 2014Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTDInventors: Ron Naaman, Eyal Capua, Danny Bavli, Maria Tkachev
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Publication number: 20140171335Abstract: Biomarker signatures were identified which can be used to diagnose or predict kidney or liver toxicity and more specifically renal tubular toxicity as a consequence of disease or drug treatment.Type: ApplicationFiled: November 27, 2013Publication date: June 19, 2014Applicant: NOVARTIS AGInventors: Gerard MAURER, Frank DIETERLE, Elias PERENTES, Frank STAEDTLER, Andre CORDIER, Andreas MAHL, Jacky VONDERSCHER, Daniel WAHL, Olivier GRENET, Daniel Robert ROTH
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Patent number: 8753847Abstract: Compositions and methods are provided for selection and enrichment of a target gene from a library of polynucleotide sequences such as might be formed from a genome or by random mutagenesis of a genetic sequence. The selection and enrichment occurs in aqueous droplets formed in an emulsion that compartmentalize individual polynucleotides from the library or a plurality of polynucleotides that may include polynucleotides not derived from the library, transcription and translation reagents and optionally additional chemical and enzyme reagents. The selection and enrichment method utilizes a polynucleotide adaptor which when ligated to the polynucleotide fragment enables amplification to occur in the presence of an adaptor specific primer.Type: GrantFiled: August 13, 2013Date of Patent: June 17, 2014Assignee: New England Biolabs, Inc.Inventors: Yu Zheng, Richard J. Roberts
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Publication number: 20140162285Abstract: Described are methods of identifying compounds useful for modulating vesicular trafficking, particularly those that disrupt the interactions between ARNO and the V-ATPase a2-subunit. Also described are peptides that modulate vesicular trafficking.Type: ApplicationFiled: December 2, 2013Publication date: June 12, 2014Applicant: The General Hospital CorporationInventors: Vladimir Marshansky, Maria Merkulova
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Publication number: 20140161769Abstract: The present invention features a method of treating or reducing the likelihood of an inflammatory autoimmune disorder (e.g., systemic lupus erythematosus (SLE)) or a kidney disorder (e.g., glomerulonephritis) in a subject by administering an inhibitor of calcium/calmodulin-dependent protein kinase type IV (CaMKIV). The invention additionally features methods of diagnosing a subject with SLE or a kidney disorder by determining the level or biological activity of a CaMKIV nucleic acid or polypeptide in a sample from a subject. Also included in the invention are methods for identifying compounds that inhibit CaMKIV for the treatment of an inflammatory autoimmune disorder (e.g., SLE) or a kidney disorder (e.g., glomerulonephritis).Type: ApplicationFiled: July 8, 2013Publication date: June 12, 2014Applicant: Beth Israel Deaconess Medical Center, Inc.Inventors: George C. Tsokos, Yuang-Taung Juang, Chun-Shin Hahn
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Publication number: 20140161791Abstract: The present invention provides for compositions and methods for diagnosing and treating Kawasaki disease. More specifically, the proteomes of patients with KD are enriched for the meprin A, filamin B, and filamin C, which serve as biomarkers (and potential therapeutic targets) for KD. Accordingly, detection of these biomarkers, using compositions and methods provided for herein, can inform the therapy delivered to the subject.Type: ApplicationFiled: April 13, 2012Publication date: June 12, 2014Applicant: CHILDREN'S MEDICAL CENTER CORPORATIONInventors: Alex Kentsis, Susan Kim, Hanno Steen
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Publication number: 20140162889Abstract: The present disclosure provides a marker for predicting prognosis of liver cancer, a composition or kit for predicting prognosis of liver cancer, preferably, a composition or kit for predicting prognosis of liver cancer for predicting the prognosis according to the stage of liver cancer. The marker, composition or kit, and method of the present disclosure make it possible to effectively predict prognosis of liver cancer, preferably, prognosis of liver cancer according to stage.Type: ApplicationFiled: September 16, 2010Publication date: June 12, 2014Inventors: Jin Young Park, Seok Joo Hong, Jong Min Kim
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Publication number: 20140162286Abstract: The present invention provides methods for detecting an enzymatic activity, the method including combining at least one magnetic particle to an enzyme substrate to form a magnetically modified substrate, reacting the magnetically modified substrate with at least one enzyme; and detecting a change in a magnetic property of the magnetically modified substrate or its cleavage products, thereby detecting an activity of said at least one enzyme, wherein the method may be applied to a human subject to detect a disease selected from the group consisting of rheumatitis, arthritis, an injury, Dupuytren's disease, Peyronie's disease, a collagen related disease, steatosis, fibrosis, cirrhosis, metastasis, tissue regeneration, cancer, coronary disease, a liver disease, a metabolic condition, an infection and an inflammatory disease.Type: ApplicationFiled: December 25, 2013Publication date: June 12, 2014Inventor: Julian DAICH
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Publication number: 20140162891Abstract: The subject invention concerns methods using a panel of proteins to detect, diagnose, and monitor therapy during treatment of ovarian cancer in a female patient. The proteins were identified using proteomics analyses of plasma samples obtained preoperatively from ovarian cancer patients versus those of healthy control women. Such a panel has utility for the diagnosis of ovarian cancer, screening for ovarian cancer and possibly therapeutic monitoring.Type: ApplicationFiled: July 22, 2013Publication date: June 12, 2014Inventors: Rebecca SUTPHEN, Johnathan Mark LANCASTER
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Publication number: 20140162893Abstract: A device for identifying the presence of a specific target molecule or biomarker by the detection of a change in an electrical property includes a measurement sensor 8 comprising a semiconducting sensor structure 12 capable of conjugating with the biomarker, thus giving rise to the said change in electrical property, and an electrode system 3, 4 for conducting a signal from the device. According to the invention there is a further such sensor 9, of substantially identical form but having its sensor structure 14 already conjugated with the biomarker, or otherwise capped, e.g. using a further oligonucleotide strand, so as to act as an internal reference. When a biological sample, e.g. saliva, is applied to the electrodes, the reference enables the discounting of all environmental effects other than the biomarker. The invention provides a simple, cheap and accurate text for one or more biomarkers that can be used in the field without complex equipment.Type: ApplicationFiled: April 2, 2012Publication date: June 12, 2014Applicant: Sapient Sensors LimitedInventors: Stephen Lee Cash, Keith Robson, Ian Anthony Kinloch, Peter George Stockley
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Patent number: 8748352Abstract: A method for identifying persons with increased risk of developing type 1 diabetes mellitus, or having type I diabetes mellitus, utilizing selected biomarkers described herein either alone or in combination. The present disclosure allows for broad based, reliable, screening of large population bases. Also provided are arrays and kits that can be used to perform such methods.Type: GrantFiled: August 15, 2012Date of Patent: June 10, 2014Assignee: Battelle Memorial InstituteInventors: Qibin Zhang, Thomas O. Metz