Saccharide (e.g., Dna, Etc.) Patents (Class 436/94)
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Publication number: 20110189674Abstract: The present invention relates to a method for quantifying or detecting DNA having a target DNA region, and so on.Type: ApplicationFiled: August 18, 2009Publication date: August 4, 2011Applicant: SUMITOMO CHEMICAL COMPANY, LIMITEDInventors: Yoshitaka Tomigahara, Hideo Satoh, Hirokazu Tarui
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Publication number: 20110189782Abstract: An apparatus for processing at least one biological sample accommodated on at least one carrier member (15) in a chamber includes, at least one reservoir (18) able to accommodate a fluid on a surface inside the chamber adjacent to and/or facing a substantial part of the at least one biological sample. The apparatus may comprise a bottom member (12) arranged to support at least one carrier member (15) carrying at least one biological sample and a lid (14) including at least one fluid reservoir (18). The reservoir filled with water provides humidity to the chamber and impedes drying out of the sample.Type: ApplicationFiled: January 28, 2011Publication date: August 4, 2011Applicant: DakoCytomation Denmark A/SInventors: Gregory A. Testa, Tim Svenstrup Poulsen, Steen Hauge Matthiesen, Ole Feldballe Rasmussen, Lars Winther
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Patent number: 7989215Abstract: The present invention relates to methods and systems for adding a reagent to an analyte in a gel. The invention further provides methods and systems for transferring liquid analyte reagent mixtures from a gel to a second vessel, such as a microtitre plate. The invention is useful in the manipulation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for manipulating proteins and peptides in isoelectric focusing gels.Type: GrantFiled: June 9, 2006Date of Patent: August 2, 2011Assignee: GE Healthcare Bio-Sciences ABInventors: Jonas Astrom, Bengt Bjellqvist, Lars Fagerstam, Kristina Uhlen
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Patent number: 7989214Abstract: A microreactor includes a shell structure (2, 3), having a bottom wall (2) and a peripheral wall (3); a layer (5), accommodated in the shell structure (2, 3) and having cavities (9, 10) formed therein, the cavities being accessible form outside the shell structure (2, 3); reagents (17), arranged between the bottom wall (2) and the layer (5), at locations corresponding to the cavities (9, 10). The layer (5) is made of a meltable material that is solid at room temperature, has a melting point (TMP) lower than a maximum operative temperature (TMAX) required by reactions performable through the microreactor (1) and is not miscible with water. The melting point (TMP) may be between 50° C. and 70° C. In one embodiment, the melting point (TMP) is lower than a minimum operative temperature (TMIN) required by reactions performable through the microreactor (1).Type: GrantFiled: December 28, 2009Date of Patent: August 2, 2011Assignee: STMicroelectronics S.r.l.Inventors: Marco Angelo Bianchessi, Alessandro Cocci
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Publication number: 20110183056Abstract: Nobel Stevia Sweetening components are provided. Through the analysis of the components of the nobel Steviol Glycoside included in the stevia extract and/or crystals, not only the quality control of sweeteners, but judgment on the correctness of indication of origin or infringement of right are facilitated since the raw material of the sweetener can be identified.Type: ApplicationFiled: October 2, 2009Publication date: July 28, 2011Inventors: Toyoshige Morita, Isao Fujita, Fumito Matsuura, Masaya Ota
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Publication number: 20110182901Abstract: The invention relates to a full length Large T tumor antigen of Merkel Cell Polyomavirus (MCV) as a therapeutic target in Merkel Cell Carcinoma (MCC).Type: ApplicationFiled: January 11, 2011Publication date: July 28, 2011Applicant: INSTITUT CURIEInventors: Martine PETER, Xavier SASTRE-GARAU
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Publication number: 20110182980Abstract: An object of the present invention is to provide a composition for suppressing expression of a target gene, and the like. A composition comprising an RNA-encapsulated liposome, wherein the RNA contains a sequence consisting of 15 to 30 contiguous bases of a target gene mRNA (hereinafter, sequence X) and a base sequence (hereinafter, complementary sequence X?) complementary to the sequence X, 1 to 90% of all sugars binding to the bases of the sequence X and the complementary sequence X? being ribose substituted by a modifying group at 2? position, and the lipid particle being capable of reaching a tissue or an organ containing an expression site of the target gene is provide.Type: ApplicationFiled: January 31, 2011Publication date: July 28, 2011Inventors: Nobuhiro Yagi, Junichi Enokizono
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Publication number: 20110185448Abstract: Methods for conveying soybean rust (SBR) resistance into non-resistant soybean germplasm are provided. In some embodiments, the methods include introgressing SBR resistance into a non-resistant soybean using one or more nucleic acid markers for marker-assisted breeding among soybean lines to be used in a soybean breeding program, wherein the markers are linked to and/or associated with SBR resistance. Also provided are single nucleotide polymorphisms (SNPs) associated with resistance to SBR; soybean plants, seeds, and tissue cultures produced by any of the disclosed methods; seed produced by the disclosed soybean plants; and compositions including amplification primer pairs capable of initiating DNA polymerization by a DNA polymerase on soybean nucleic acid templates to generate soybean marker amplicons.Type: ApplicationFiled: July 17, 2009Publication date: July 28, 2011Inventors: Ju-kyung Yu, Becky Breitinger, Glenn Bowers, Nanda Chakraborty
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Publication number: 20110183019Abstract: Disclosed are methods of screening for an autism spectrum disorder, compositions that inhibit the release of molecules that disrupt the blood-brain barrier, compositions for treating autism spectrum disorders, and methods of treating autism spectrum disorders.Type: ApplicationFiled: January 19, 2011Publication date: July 28, 2011Applicant: Theta Biomedical Consulting & Development Co., IncInventor: Theoharis C. THEOHARIDES
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Publication number: 20110177493Abstract: A molecular sensor is provided that contains at least one carbon nanotube suspended on a suitable support structure. In one aspect, at least one receptor is attached to a surface of the suspended carbon nanotube. Also provided are methods of detecting an analyte in a sample by contacting a sample suspected of containing the analyte with the molecular sensor of this invention under suitable conditions that favor binding of the analyte to the receptor and detecting any analyte bound to the receptor, if present.Type: ApplicationFiled: February 13, 2009Publication date: July 21, 2011Inventor: Jennifer Lu
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Publication number: 20110175982Abstract: An analysis of an object dyed with fluorescent coloring agents carried out with the aid of a fluorescent microscope which is modified for improved resolving power and called a nanoscope. The method is carried out with a microscope having an optical system for visualizing and projecting a sample image to a video camera which records and digitizes images of individual fluorescence molecules and nanoparticles at a low noise, a computer for recording and processing images, a sample holder arranged in front of an object lens, a fluorescent radiation exciting source and a set of replaceable suppression filters for separating the sample fluorescent light.Type: ApplicationFiled: September 27, 2010Publication date: July 21, 2011Inventors: Andrey Alexeevich Klimov, Dmitry Andreevich Klimov, Evgeniy Andreevich Klimov, Tatiana Vitalyevna Klimova
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Publication number: 20110177494Abstract: The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.Type: ApplicationFiled: February 9, 2011Publication date: July 21, 2011Applicant: The University of ChicagoInventors: Rustem F. Ismagilov, Joshua David Tice, Cory John Gerdts, Bo Zheng
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Publication number: 20110179501Abstract: Methods for restoring a desired pattern of DNA methylation, inducing re-expression of methylation-silenced tumor suppressor genes (TSGs), and/or inhibiting tumorigenicity both in vitro and in vivo in a subject in need thereof by administering an effective amount of one or more miR-29s sufficient to target one or more of DNMT3A and DNMT3B are disclosed.Type: ApplicationFiled: July 30, 2008Publication date: July 21, 2011Applicant: THE OHIO STATE UNIVERSITY RESEARCH FOUNDATIONInventors: Carlo M. Croce, Muller Fabbri
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Publication number: 20110171264Abstract: An aqueous composition which comprises a protein or other biological molecule and also (i) optionally comprises one or more metal ions at a concentration of 0.01 to 20 mM; (ii) comprises excipients which are weak ligands; and (iii) is substantially free of excipients which are medium-strength ligands or strong ligands.Type: ApplicationFiled: October 28, 2010Publication date: July 14, 2011Inventor: Jan Jezek
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Publication number: 20110171738Abstract: The present invention provides a method for estimating an amount of immobilized probes, including the successive steps of: providing a sample on a substrate to form one or more spots of the sample on the substrate, the sample containing particulate substances and probes in a predetermined ratio, the probes being reactive with a predetermined target; measuring the number of the particulate substances contained in at least one of the spots; and estimating the amount of the probes contained in the at least one of the spots from the thus measured number of the particulate substances.Type: ApplicationFiled: January 5, 2011Publication date: July 14, 2011Applicant: RIKENInventors: Hiroyoshi AOKI, Yutaka YAMAGATA, Naoko KODAMA
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Publication number: 20110171629Abstract: The present invention provides compositions and devices comprising nanostructure networks, and related methods. The compositions may exhibit enhanced interaction between nanostructures, providing improved device performance (e.g., improved conductivity). In some embodiments, the devices are capable of interacting with various species to produce an observable signal from the device. In some cases, the compositions and devices may be useful in the determination of analytes, including—biological analytes (e.g., DNA, ebola virus, other infective agents, etc.), small, organic analytes, and the like. The embodiments described herein may exhibit high sensitivity and specificity to analytes and may be capable of analyte detection at femtomolar concentrations (e.g., 10 fM).Type: ApplicationFiled: November 4, 2010Publication date: July 14, 2011Applicant: Massachusetts Institute of TechnologyInventors: Timothy M. Swager, Yossef Weizmann, David M. Chenoweth
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Publication number: 20110171740Abstract: Polymeric sequence probes and methods are described that enhance the speed and sensitivity of detection of target analytes by combining a multiplicity of binding moieties specific for analyte, at least two of which are linearly arranged and optionally a multiplicity of detectable labels.Type: ApplicationFiled: April 19, 2010Publication date: July 14, 2011Inventors: YONG CHEN, SUXIAN HUANG
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Publication number: 20110171671Abstract: Chemiluminescent detection of metabolic by-products of inosine and hypoxanthine is used to diagnose ischemic events such as early acute cardiac ischemia.Type: ApplicationFiled: August 1, 2008Publication date: July 14, 2011Inventors: Don Farthing, Lei Xi, Thomas H. Karnes, Domenic Sica, Todd Gehr
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Publication number: 20110171741Abstract: The present invention relates, e.g., to a method for determining the size distribution of DNA molecules in a sample comprising cell-free nucleic acid, comprising labeling the DNA with a fluorescent dye in a stoichiometric manner, subjecting the DNA to molecular spectroscopy (e.g., cylindrical illumination confocal spectroscopy), analyzing suitable fluorescent burst parameters of the labeled DNA, and conducting single molecule DNA integrity analysis of the labeled DNA molecules in the sample. In one embodiment of the invention, the method is used as a diagnostic method for detecting cancer.Type: ApplicationFiled: November 4, 2009Publication date: July 14, 2011Applicant: The Johns Hopkins UniversityInventors: Tza-Huei Wang, Kelvin J. Liu, Ie-Ming Shih
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Publication number: 20110171678Abstract: Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. The invention includes derivative compounds having one or more benzo nitrogens.Type: ApplicationFiled: October 18, 2010Publication date: July 14, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Wai-Yee Leung, Ching-Ying Cheung, Stephen Yue
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Patent number: 7977112Abstract: A method of measuring an analyte in a biological fluid comprises applying an excitation signal having a DC component and an AC component. The AC and DC responses are measured; a corrected DC response is determined using the AC response; and a concentration of the analyte is determined based upon the corrected DC response. Other methods and devices are disclosed.Type: GrantFiled: December 9, 2008Date of Patent: July 12, 2011Assignees: Roche Diagnostics Operations, Inc., Roche Operations Ltd.Inventors: David W. Burke, Lance S. Kuhn, James Maxwell
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Patent number: 7977109Abstract: The present invention relates to a method for enriching nucleic acids with a length of not more than 300 nucleotides. The invention also relates to a kit for enriching nucleic acids with a length of not more than 300 nucleotides, to the use of such a kit, to the use of an anion exchange matrix and to a method for treating a disease.Type: GrantFiled: December 8, 2006Date of Patent: July 12, 2011Assignee: Qiagen GmbHInventors: Christoph Ritt, Ralf Himmelreich, Martin Weber
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Patent number: 7977108Abstract: The present invention provides methods and probe nucleic acids for detecting mutant forms of target nucleic acids that comprise repetitive nucleotide sequences. In certain embodiments, for example, these approaches and reagents can be used to detect instability in regions of genomic DNA that include microsatellite markers. The invention also provides related reaction mixtures, systems, and kits.Type: GrantFiled: July 13, 2006Date of Patent: July 12, 2011Assignee: Roche Molecular Systems, Inc.Inventors: Christopher D. Newhouse, Stephen Gordon Will, Amar P. Gupta
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Publication number: 20110165560Abstract: The present invention provides methods, compositions, and kits associated with succinate dehydrogenase-related disease conditions. In one aspect of the present invention, a method for screening a test subject to determine risk for developing a succinate dehydrogenase-related disease condition is provided. Such a method can include obtaining a biological sample from the test subject and identifying a mutation in gene hSDH5 from the biological sample of the test subject, wherein the mutation effectuates a decreased level of succinate dehydrogenase flavination in the test subject as compared to a level of succinate dehydrogenase flavination in a normal subject. In one aspect, the mutation is at hSDH5 Gly78 of gene hSDH5. In another aspect, the mutation is equivalent to an hSDH5 Gly78Arg substitution of gene hSDH5.Type: ApplicationFiled: July 6, 2010Publication date: July 7, 2011Inventors: JARED RUTTER, HUAIXIANG HAO, JOSHUA SCHIFFMAN
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Publication number: 20110166082Abstract: A method and compound for treating skeletal muscle mass deficiency in a human subject are disclosed. The composition is an oligomer of morpholino subunits and phosphorus-containing intersubunit linkages joining a morpholino nitrogen of one subunit to a 5? exocyclic carbon of an adjacent subunit, contains between 10-40 nucleotide bases, has a base sequence effective to hybridize to an expression-sensitive region of processed or preprocessed human myostatin RNA transcript, identified, in its processed form, by SEQ ID NO:6, and is capable of uptake by target muscle cells in the subject. In practicing the method, the compound is administered in an amount and at a dosage schedule to produce an overall reduction in the level of serum myostatin measured in the patient, and preferably to bring the myostatin level within the a range determined for normal, healthy individuals.Type: ApplicationFiled: January 3, 2011Publication date: July 7, 2011Applicant: AVI BioPharma, Inc.Inventors: Patrick L. Iversen, Dwight D. Weller, Alan P. Timmins
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Publication number: 20110165586Abstract: Provided is a detection method of a biochemical material using surface-enhanced Raman scattering in order to detect the existence of a biochemical material in a target subject or its content therein, more particularly a detection method of a biochemical material facilitating multiplex detection with high-sensitivity, high-reproducibility, high-reliability, and high-precision owing to multiple hot spots formed on the nanowire surface of a single crystal body by the bond of multiple nanoparticles which are physically separated from each other.Type: ApplicationFiled: October 7, 2010Publication date: July 7, 2011Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Bongsoo Kim, Taejoon Kang, Ilsun Yoon, Sang Yup Lee, Seung Min Yoo
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Publication number: 20110165691Abstract: Novel mono-azide substituted rylene-imide derivatives, their use in methods for the detection of analytes and reagents kits for the detection of analytes comprising said novel mono-azide substituted rylene-imide derivatives.Type: ApplicationFiled: January 14, 2011Publication date: July 7, 2011Applicant: BASF SEInventors: Thomas Geßner, Helmut Reichelt, Ingo Münster, Martin Könemann, Neil Gregory Pschirer, Jian Qiang Qu, Rüdiger Sens, Anja Schwögler, Antonio Manetto
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Publication number: 20110165557Abstract: Provided are an apparatus and method for detecting biomolecules. The apparatus includes a FET having a substrate, a source electrode, a drain electrode, a channel region between the source and drain electrodes, and probe molecules fixed to the channel region, wherein the source and drain electrodes are separated on the substrate, a microfluid supplier selectively supplying one of a reference buffer solution of low ionic concentration and a reaction solution of high ionic concentration containing target molecules, to the channel region of the FET to which the probe molecules are fixed, and a biomolecule detector detecting the target molecules by measuring a first current value of the channel region of the FET, and a second current value of the channel region of the FET to which the target molecules and the probe molecules that bind to each other in the reaction solution of high ionic concentration are fixed.Type: ApplicationFiled: November 20, 2008Publication date: July 7, 2011Applicant: Electronics and Telecommunications Research InstituteInventors: Chil-Seong Ah, Ansoon Kim, Chan-Woo Park, Chang-Geun Ahn, Jong-Heon Yang, In-Bok Baek, Taeyoub Kim, HyeKyoung Yang, Gun-Yong Sung, Seon-Hee Park, Han-Young Yu, Moon-Gyu Jang
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Publication number: 20110165690Abstract: Disclosed herein is a composition for molecular imaging comprising a trans-splicing ribozyme coupled with an imaging reporter gene. The trans-splicing ribozyme targets a specific gene associated with a disease. Also disclosed is a molecular imaging method using the composition.Type: ApplicationFiled: October 26, 2007Publication date: July 7, 2011Inventors: In-Hoo Kim, Seong-Wook Lee, Seung-Hee Hong, Jin-Sook Jeong, Yeon-Su Lee, Haeng-Im Jung
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Publication number: 20110165148Abstract: A novel gene (designated 162P1E6) and its encoded protein, and variants thereof, are described wherein 162P1E6 exhibits tissue specific expression in normal adult tissue, and is aberrantly expressed in the cancers listed in Table I. Consequently, 162P1E6 provides a diagnostic, prognostic, prophylactic and/or therapeutic target for cancer. The 162P1E6 gene or fragment thereof, or its encoded protein, or variants thereof, or a fragment thereof, can be used to elicit a humoral or cellular immune response; antibodies or T cells reactive with 162P1E6 can be used in active or passive immunization.Type: ApplicationFiled: March 8, 2011Publication date: July 7, 2011Applicant: AGENSYS, INC.Inventors: Pia M. Challita-Eid, Arthur B. Raitano, Mary Faris, Rene S. Hubert, Karen Jane Meyrick Morrison, Robert Kendall Morrison, Wangmao Ge, Aya Jakobovits
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Patent number: 7972859Abstract: A method of determining authenticity of an ingestible product purportedly from a batch of saccharide-marked ingestible product is provided. The ingestible product is a food, beverage, or pharmaceutical, for example. The method includes identifying a sample of the ingestible product to be tested; and testing the sample for a marked presence of the saccharide using a saccharide-specific binding protein. The ingestible product is not authentic when the testing demonstrates an unexpected change in the marked presence of the saccharide, and the ingestible product is authentic when the testing demonstrates no unexpected change in the marked presence of the saccharide.Type: GrantFiled: June 26, 2007Date of Patent: July 5, 2011Assignee: Authentix, Inc.Inventors: Ian Eastwood, Jared Cartwright, Erwin Dorland, Mark Saw
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Patent number: 7972860Abstract: The present invention relates to a novel integrated PCR-free signal amplification polynucleotide detection system which combines a specific receptor, an optical transducer, and an amplification mechanism. This novel detection system is based on different electrostatic interactions and confirmations between a cationic polythiophene (i.e., polymer 1) and single-stranded or double-stranded polynucleotides (such as ss-DNA or ds-DNA), and the efficient energy transfer between the triplex (complexation between the cationic polythiophene and ds-DNA) and neighboring fluorophores attached to ss-DNA or ds-DNA probes. It is to be understood that in the case of ss-DNA, triplex formation occurs via the hybridization of complementary ss-DNA strands, combined with complexation and with the cationic polythiophene. The present detection system allows for the detection of single nucleotide polymorphisms (SNPs) from samples in only a few minutes, without the need for nucleic acid amplification.Type: GrantFiled: March 3, 2006Date of Patent: July 5, 2011Assignee: National Research Council of CanadaInventors: Mario LeClerc, Hoang-Anh Ho
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Patent number: 7972858Abstract: Described herein are methods for analyzing polymer molecules. These methods are employed for the high throughput readout of DNA and RNA molecules with single molecule sensitivity. The method of the present invention comprises (1) the electrically controlled unzipping of DNA (or RNA) double strands, and (2) the readout of the molecule's identity (or code) using one or more molecule signal detection.Type: GrantFiled: August 12, 2005Date of Patent: July 5, 2011Assignee: President and Fellows of Harvard CollegeInventors: Amit Meller, Jerome Mathe, John S. Eid
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Publication number: 20110159601Abstract: The invention features methods for evaluating the conformation of a polymer, for example, for determining the conformational distribution of a plurality of polymers and to detect binding or denaturation events. The methods employ a nanopore which the polymer, e.g., a nucleic acid, traverses. As the polymer traverses the nanopore, measurements of transport properties of the nanopore yield data on the conformation of the polymer.Type: ApplicationFiled: December 6, 2010Publication date: June 30, 2011Inventors: Jene A. Golovchenko, Jiali Li, Derek Stein, Marc H. Gershow
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Publication number: 20110159602Abstract: Salmonella typhi Vi saccharide can be assayed in two new ways. First, its proton NMR spectrum can be used, with comparison to an internal Standard permitting quantitative analysis. Second, anion exchange chromatography with amperometric detection can be used on hydrolysed saccharide.Type: ApplicationFiled: June 12, 2009Publication date: June 30, 2011Inventors: Francesco Berti, Francesca Micoli, Daniela Proietti
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Publication number: 20110159011Abstract: Methods of detecting inflammatory disorders using IL- 1 isoforms are provided. Methods of treating an inflammatory disorder with an anti-IL-1 antibody are also provided. Methods of treating an inflammatory disorder with an anti-IL-1 antibody and at least one of an anti-IL-22 antibody, an anti-IL-17 antibody, or an anti-TNF? antibody are also provided.Type: ApplicationFiled: August 28, 2009Publication date: June 30, 2011Applicant: Wyeth LLCInventors: Yijun Carrier, Hak-Ling Ma, Kyriaki Dunussi-Joannopoulos, Quintus G. Medley
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Publication number: 20110160290Abstract: Stable, extracellular microRNAs and methods for isolating and identifying such microRNAs from a body fluid are provided. The extracellular microRNAs isolated from a bodily fluid of a subject can be used to measure disease and provide sensitive, efficient, and non invasive methods for the detection of disease, including cancer. The extracellular microRNAs can be used to develop new therapeutics for the treatment of disease, including cancer. The examples illustrate diagnosis of prostate and ovarian cancer and differential expression of miR-100, miR-135b, miR-141, miR-148a, miR-200a, miR-200c, miR-210, miR-222, miR-375, miR-425-Sp and miR-429.Type: ApplicationFiled: May 21, 2009Publication date: June 30, 2011Inventor: Muneesh Tewari
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Publication number: 20110159476Abstract: The invention provides methods and apparatus for characterizing complex polymeric mixture of interest. Candidate solutions are eliminated from a solution space using one or more experimental measurements of a polymeric mixture of interest. The elimination step can be repeated one or more times using different experimental measurements produced by various chemical and physical protocols, so that the remaining candidate solutions converge to describe the actual polymeric mixture under investigation. Once the composition of the complex polymeric mixture has been characterized, the information thus generated can be used to facilitate, for example, the manufacture of a bio-equivalent of the complex polymeric mixture.Type: ApplicationFiled: September 3, 2010Publication date: June 30, 2011Inventor: SASISEKHARAN RAGURAM
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Publication number: 20110158977Abstract: The present invention provides variant LcrE sequences and/or combinations of variant LcrE sequences across the Chlamydia trachomatis serovars. Such changes in LcrE genotypes across the Chlamydia trachomatis serovars are likely to correspond with changes in LcrE phenotypes, in particular, with changes in immunogenicity. The present invention also provides peptides surrounding or associated with the amino acid substitutions, in particular, peptides surrounding or associated with high frequency mutated amino acid positions or hypervariable regions, which are likely to be B and/or T cell epitopic regions capable of eliciting Chlamydia specific cell mediated immune responses. The variant LcrE sequences and/or combinations of the variant LcrE sequences and/or epitope regions associated with the variant LcrE sequences are useful as immunogens and/or in the preparation of immunogenic compositions for preventing and/or treating and/or, diagnosing Chlamydia infections.Type: ApplicationFiled: May 12, 2006Publication date: June 30, 2011Applicant: Novartis Vaccines and Diagnostics, Inc.Inventors: Guido Grandi, Giulio Ratti
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Patent number: 7968327Abstract: This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solublize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.Type: GrantFiled: August 14, 2007Date of Patent: June 28, 2011Inventors: Gary McMaster, Joan Davies, Yunqing Ma, Yuling Luo
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Patent number: 7968345Abstract: A solid-phase permethylation procedure is described. For example, solid-phase permethylation can be utilized to prepare permethylated linear and branched, neutral and sialylated oligosaccharides, which can be analyzed by MALDI-MS.Type: GrantFiled: August 10, 2010Date of Patent: June 28, 2011Assignee: Indiana University Research and Technology CorporationInventors: Pilsoo Kang, Yehia S. Mechref, Milos V. Novotny
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Publication number: 20110154526Abstract: The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-043A47-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.Type: ApplicationFiled: December 16, 2010Publication date: June 23, 2011Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E.I. DU PONT DE NEMOURS AND COMPANYInventors: Scott Diehn, Albert L. Lu, Timothy M. Nowatzki, Douglas S. Nubel, M. Alejandra Pascual, James C. Register, III, Christopher J. Scelonge, Gregory J. Young, Joshua K. Young, Cathy Xiaoyan Zhong
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Publication number: 20110150916Abstract: Immunogenic compositions and vaccines are described comprising GAS Markers including AtmB Proteins. Methods for detecting GAS diseases in a subject are also described comprising measuring GAS markers or antibodies against GAS markers in a sample from the subject. The invention further provides kits for carrying out the methods of the invention and therapeutic applications for GAS diseases employing GAS markers, polynucleotides encoding the markers, and/or binding agents for the markers.Type: ApplicationFiled: November 15, 2007Publication date: June 23, 2011Applicant: MOUNT SINAI HOSPITALInventors: Kowthar Salim, Joyce De Azavedo, Dennis Cvitkovitch
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Publication number: 20110152346Abstract: The invention is concerned with the use of oligonucleotide analogs that contain specifically modified DNA bases to be used in hybridization of nucleic acids, polymerase chain reaction (PCR) and siRNA-mediated gene silencing (RNAi).Type: ApplicationFiled: November 5, 2008Publication date: June 23, 2011Applicant: Baltic Technology Development Ltd.Inventors: Mati Karleson, Erkki Truve, Allan Olspert, Cecilia Sarmiento, Mart Saarma
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Publication number: 20110150860Abstract: The invention provides isolated nucleic acids molecules, designated 25869, 25934, 26335, 50365, 21117, 38692, 46508, 16816, 16839, 49937, 49931 and 49933 nucleic acid molecules. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing 25869, 25934, 26335, 50365, 21117, 38692, 46508, 16816, 16839, 49937, 49931 or 49933 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a 25869, 25934, 26335, 50365, 21117, 38692, 46508, 16816, 16839, 49937, 49931 or 49933 gene has been introduced or disrupted. The invention still further provides isolated 25869, 25934, 26335, 50365, 21117, 38692, 46508, 16816, 16839, 49937, 49931 or 49933 proteins, fusion proteins, antigenic peptides and anti-25869, 25934, 26335, 50365, 21117, 38692, 46508, 16816, 16839, 49937, 49931 or 49933 antibodies. Diagnostic and therapeutic methods utilizing compositions of the invention are also provided.Type: ApplicationFiled: April 7, 2010Publication date: June 23, 2011Applicant: Millennium Pharmaceuticals, Inc.Inventors: Rory A.J. Curtis, Thomas Joseph Logan, Maria Alexandra Glucksmann, Rachel E. Meyers, Mark J. Williamson, Laura A. Rudolph-Owen, Miyoung Chun, Fong-Ying Tsai
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Publication number: 20110151569Abstract: The present disclosure relates to the identification of components in a sample based on the chemical nature of the component. In particular, although not exclusively, the present disclosure relates to the identification of hydrophobic and hydrophilic components using a mass spectrometric technique. The present invention also relates to kits and products used in the identification of the components, as well as other subject matter.Type: ApplicationFiled: August 20, 2009Publication date: June 23, 2011Applicants: ROAR PARTICLES UK LIMITED, NANOFRONTIER PTE LTDInventors: Frederick Rowell, Jan Ma, Angelina Yi Mei Lim
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Publication number: 20110151429Abstract: The present invention relates to a bioprobe including a substrate and inorganic nanoparticles attached to the surface of the substrate, a method of preparing the bioprobe, and an analysis apparatus and method using the bioprobe. In the bioprobe according to the present invention, inorganic nanoparticles introduced to the substrate serve as a linker to which a target-specific substance such as an antibody can be bound, and they also increase the surface area of the substrate, thus increasing a surface area where a target substance to be detected can contact the substrate. In this regard, the bioprobe can be effectively used for detection, dosing, or analysis of various biomolecules or other chemical substances.Type: ApplicationFiled: March 31, 2009Publication date: June 23, 2011Applicant: Industry-Academic Cooperation Foundation,Yonsei UniversityInventors: Seungjoo Haam, Jin-Suck Suh, Yong-Min Huh, Jaemoon Yang, Eun-Kyung Lim, Yoonah Kang
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Publication number: 20110154524Abstract: The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-032316-8 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.Type: ApplicationFiled: December 16, 2010Publication date: June 23, 2011Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E.I. DU PONT DE NEMOURS AND COMPANYInventors: Scott Diehn, Albert L. Lu, Timothy M. Nowatzki, Douglas S. Nubel, M. Alejandra Pascual, James C. Register, III, Christopher J. Scelonge, Gregory J. Young, Joshua K. Young, Cathy Xiaoyan Zhong
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Publication number: 20110152107Abstract: The invention provides a porous nanoscale membrane. In one embodiment, the membrane can be used as a filtration device to screen agents that disrupt or prevent molecular interactions. In one embodiment, the membrane allows for screening agents that disrupt or prevent molecular interactions using a small sample volume with efficient high-throughput screening applications.Type: ApplicationFiled: September 9, 2010Publication date: June 23, 2011Inventors: James L. McGrath, Harold C. Smith
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Publication number: 20110154525Abstract: The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-040416-8 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.Type: ApplicationFiled: December 16, 2010Publication date: June 23, 2011Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E.I. DU PONT DE NEMOURS AND COMPANYInventors: Scott Diehn, Albert L. Lu, Timothy M. Nowatzki, Douglas S. Nubel, M. Alejandra Pascual, James C. Register, III, Christopher J. Scelonge, Gregory J. Young, Joshua K. Young, Cathy Xiaoyan Zhong