Separation Or Purification Patents (Class 530/344)
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Publication number: 20120295813Abstract: The present invention relates to a system and method for controlling peptide display valency on virus-like particles (VLPs), especially including MS2 VLPs. In this method, large amounts of wild-type and low quantities of single-chain dimer coat proteins may be produced from a single RNA. Valency is controlled in immunogen (vaccine) production by providing a system that allows the production of large amounts of wild-type and low quantities of single-chain dimer coating proteins from a single RNA, allowing facile adjustment of display valency levels on VLPs, especially MS2 VLPS over a wide range, from few than one-on average—to as many as ninety per particle. This facilitates the production of immunogens and vaccines, including VLPs exhibiting low valency.Type: ApplicationFiled: December 31, 2010Publication date: November 22, 2012Inventors: David S. Peabody, Bryce Chackerian
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Publication number: 20120296068Abstract: A method of purifying a compound from a mixture through a chromatographic column loaded with a column adsorbent. The method comprises: applying the mixture to the chromatographic column; eluting the mixture with an elution solvent composition; and collecting the compound; wherein at least one of the column adsorbent and elution solvent is selected based on one of solubility parameters of the compound, column adsorbent, elution solvent, and conformation energy of the compound.Type: ApplicationFiled: May 15, 2012Publication date: November 22, 2012Applicants: ScinoPharm Taiwan Ltd., National Central UniversityInventors: Wen-Yih CHEN, Li-Chiao Chang, Ruoh-Chyu Ruaan, Ching-Wei Tsai
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Publication number: 20120277407Abstract: A novel method capable of liberating a peptide from a complex of peptide and albumin and the associated method of recovering the peptide are provided. When a liquid sample containing a complex of peptide and albumin undergoes heat treatment, a self-aggregate of albumin formed in the liquid sample. The peptide is simultaneously liberated from the complex and recovered by removing the self-aggregate from the liquid sample.Type: ApplicationFiled: April 27, 2012Publication date: November 1, 2012Applicant: SYSMEX CORPORATIONInventors: Yuta YAMAMOTO, Kana KAWASAKI, Hiroyuki KABATA
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Patent number: 8288130Abstract: A palladate palladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladate palladium promoter dissolved in a high-concentration acidic organic acid solvent.Type: GrantFiled: June 7, 2010Date of Patent: October 16, 2012Assignee: Medtronic, Inc.Inventors: Jin Seog Seo, Daniel Strydom, Barton Holmquist
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Patent number: 8283443Abstract: The present invention provides a method for purifying a protein, includes the step of: contacting a fusion protein of a first protein and a second protein with a bivalent cation-containing solution, the fusion protein being adsorbed to a silicon oxide-containing substance, the first protein being capable of binding to the silicon oxide-containing substance in a solution containing 0.1M sodium chloride. With this arrangement, it is possible to easily produce large quantity of proteins which are high in purity without sacrificing activity of the proteins.Type: GrantFiled: July 9, 2009Date of Patent: October 9, 2012Assignee: Hiroshima UniversityInventors: Akio Kuroda, Takeshi Ikeda
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Patent number: 8278273Abstract: The invention relates to glucagon-related peptides and their use for the prevention or treatment of disorders involving the large intestine. In particular, it has now been demonstrated that GLP-2 and peptidic agonists of GLP-2 can cause proliferation of the tissue of large intestine. Thus, the invention provides methods of proliferating the large intestine in a subject in need thereof. Further, the methods of the invention are useful to treat or prevent inflammatory conditions of the large intestine, including inflammatory bowel diseases.Type: GrantFiled: May 6, 2011Date of Patent: October 2, 2012Assignee: 1149336 Ontario, Inc.Inventor: Daniel J. Drucker
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Publication number: 20120244594Abstract: The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide.Type: ApplicationFiled: June 28, 2007Publication date: September 27, 2012Inventors: John Rush, Hui Zhang, Xiangming Zha, Michael J. Comb, Yi Tan
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Patent number: 8268963Abstract: The present invention concerns a process for the purification of macrolide antibiotics. More specifically it concerns a process for the purification of macrolide antibiotics that result in a white powder. The powder remains white also after some time of storage. The process of the present invention is performed by dissolving the macrolide antibiotics, e.g. commercial vancomycin hydrochloride, in water and subjecting the solution to ultrafiltration with a membrane having nominal retention lower than 30,000 Da, preferably of 10,000 Da. The purified solution is preferably concentrated by reversed osmosis and then lyophilized at the optimized conditions of pressure and temperature to obtain a white powder.Type: GrantFiled: December 7, 2006Date of Patent: September 18, 2012Assignee: Xellia Pharmaceuticals ApSInventor: Vincenzo De Tommaso
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Patent number: 8241692Abstract: Canola protein isolates consisting predominantly of 7S canola proteins are formed by isoelectric precipitation from aqueous salt solution extracts of canola oil seed meal. Canola protein isolates consisting predominantly of 2S canola protein are recovered from supernatant from the isoelectric precipitation step.Type: GrantFiled: September 21, 2006Date of Patent: August 14, 2012Assignee: Burcon Nutra Science (MB) Corp.Inventors: Kevin I. Segall, Randy Willardsen, Martin Schweizer
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Publication number: 20120202244Abstract: The present invention provides for an antibody or fragment thereof capable of specifically binding to an epitope of the amino acid sequence CDPAFLYKVVD (SEQ ID NO:1) or a fragment of at least 5, 6, or 7 amino acids thereof.Type: ApplicationFiled: October 14, 2011Publication date: August 9, 2012Applicants: Carnegie Institution of Washington, The Regents of the University of CaliforniaInventors: Dominique Loque, Wolf B. Frommer
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Patent number: 8232369Abstract: The present invention relates to a method for precipitation of peptide where the mixing step of the peptide with the precipitation aid and the precipitation itself are specially separated.Type: GrantFiled: November 27, 2007Date of Patent: July 31, 2012Assignees: Talecris Biotherapeutics, Inc., Bayer Technology Services GmbHInventors: Hans-Jürgen Henzler, Stephan Kirchner, Dirk Havekost, Klaus Kaiser, Jörg Kauling, Richard Pasquinelli, Rolf Treckmann
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Patent number: 8227207Abstract: The invention relates to the production of ovoproducts containing bioactive peptides from the egg white subjected to enzymatic treatment. Said peptides have an inhibiting activity of the angiotensin converting enzyme (ACE inhibiting activity) in vitro and/or anti-hypertensive activity in rats and/or antioxidant activity. Said ovoproducts, complete hydrolyzates, the fractions thereof with low molecular weight or their constituent peptides could be used as therapeutic substances with ACE inhibiting activity and/or anti-hypertensive activity and/or anti-oxidant activity, either as functional food products, food additives or ingredients or pharmaceutical products for the treatment and/or prevention of hypertension in all its forms in humans or animals and for the treatment and/or prevention of any disorder associated with hypertension in humans or animals.Type: GrantFiled: January 30, 2006Date of Patent: July 24, 2012Assignee: Consejo Superior de Investigaciones CientifcasInventors: Marta Miguel Castro, Rosina Lopez-Alonso Fandiño, Maria Isidra Recio Sanchez, Maria Mercedes Ramos Gonzalez, Amaya Aleixandre De Artiñano
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Publication number: 20120175307Abstract: Multi-layered macromolecules wherein the layers are covalently bonded together and wherein the macromolecules are covalently bonded to solid particulate substrates, methods for the preparation of such compositions, and methods for their uses in a multitude of end use applications ranging from the purification of waste chemical and metal process streams to the separation and identification of proteins, peptides, and oligionucleotides.Type: ApplicationFiled: December 31, 2011Publication date: July 12, 2012Inventors: Charles E. Skinner, William Henry Campbell, Yung K. Kim
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Patent number: 8207298Abstract: The invention relates to methods for separating or purifying biopolymer conjugated molecules from unconjugated molecules. In particular, methods are described for purifying a PEGylated protein or oligonucleotide from an unPEGylated protein or oligonucleotide, respectively. The methods are quick and efficient separation methods because they do not require gradient chromatography, fractionation of an eluent or analysis of the eluted fractions. Further, the methods increase yield and purity of the biopolymer conjugated molecule.Type: GrantFiled: May 1, 2009Date of Patent: June 26, 2012Assignee: Archemix Corp.Inventors: Paul Hatala, William J. Issa
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Publication number: 20120149872Abstract: The present invention relates to a device for purifying an analyte from a fluid sample. The device comprises a channel or tubing having an inner surface that binds to the analyte of interest in the fluid sample. As the fluid sample flows through the channel, the analyte of interest binds to the inner wall of the channel. The bound analyte is then eluted using a small bolus of elution buffer. The channel generates a high surface area for capturing the analyte in a large volume sample, but allows low liquid elution volume for concentrating the analyte into a small volume.Type: ApplicationFiled: December 26, 2007Publication date: June 14, 2012Inventor: Phillip Belgrader
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Publication number: 20120149868Abstract: The invention relates to an effective process for purifying a peptide which has been prepared by solid phase peptide synthesis. Also encompassed by the invention is a kit comprising reagents for said process and the purified peptide obtained by said process.Type: ApplicationFiled: May 15, 2009Publication date: June 14, 2012Applicant: Novo Nordisk A/SInventors: Camilla Kornbeck, Thomas Budde Hansen
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Nucleic acid and corresponding protein entitled 193P1E1B useful in treatment and detection of cancer
Patent number: 8188228Abstract: A novel gene 0193P1E1B (also designated 193P1E1B) and its encoded protein, and variants thereof, are described wherein 193P1E1B exhibits tissue specific expression in normal adult tissue, and is aberrantly expressed in the cancers listed in Table I. Consequently, 193P1E1B provides a diagnostic, prognostic, prophylactic and/or therapeutic target for cancer. The 193P1E1B gene or fragment thereof, or its encoded protein, or variants thereof, or a fragment thereof, can be used to elicit a humoral or cellular immune response; antibodies or T cells reactive with 193P1E1B can be used in active or passive immunization.Type: GrantFiled: April 20, 2010Date of Patent: May 29, 2012Assignee: Agensys, Inc.Inventors: Arthur B. Raitano, Pia M. Challita-Eid, Mary Faris, Rene S. Hubert, Wangmao Ge, Aya Jakobovits -
Publication number: 20120122759Abstract: Methods for purifying a polypeptide from a composition comprising the polypeptide and at least one contaminant are described, which methods comprise the sequential steps of: (a) passing the composition through an ion exchange membrane, where the polypeptide and the membrane have opposite charge, at operating conditions comprised of a buffer having a pH sufficiently distinct from the pI of the polypeptide to enhance the charge of the polypeptide and a low ionic strength effective to prevent the shielding of charges by buffer ions, which cause the membrane to bind the polypeptide and the at least one contaminant, and (b) recovering the purified polypeptide from the effluent.Type: ApplicationFiled: August 14, 2008Publication date: May 17, 2012Inventors: Arick Brown, Jerome Bill, JR., Timothy Tully, Christopher Dowd
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Publication number: 20120123089Abstract: The instant disclosure provides a method for purification of peptides by chromatographic techniques. The proposed methodology will help in addressing the problems associated in purifying biological protein products emerging from the evolving biotechnology industry.Type: ApplicationFiled: July 8, 2010Publication date: May 17, 2012Applicant: BIOCON LIMITEDInventors: Nitesh Dave, Devesh Radhakrishnan, Sundaresh Shankar, Krishanachaitanya Gulla, Harish Iyer
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Publication number: 20120123091Abstract: The current invention is directed to a chromatography column separator which separates the chromatography column in an upper chromatography column chamber and a lower chromatography column chamber, and which has a variable position within the chromatography column, and which is embedded by the chromatography material. The separator allows the replacement of the chromatography material in the upper chromatography column chamber without the need to replace the chromatography column material in the lower chromatography column chamber and it allows also the combination of two different chromatography materials with different chromatographical functional groups in one chromatography column.Type: ApplicationFiled: July 28, 2010Publication date: May 17, 2012Inventors: Andreas Blaschyk, Roberto Falkenstein
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Patent number: 8163872Abstract: A novel and improved method for purification of glycopeptides, especially glycopeptide antibiotics. The method comprises contacting a solution of the glycopeptide to an ion exchange chromatography material. The product of this method has a surprisingly high purity.Type: GrantFiled: October 27, 2005Date of Patent: April 24, 2012Assignee: Xellia Pharmaceuticals APSInventors: Lene Aassveen, Kamilla Lundhaug, Kjersti Aastorp Hirth
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Patent number: 8138306Abstract: The present invention relates to a method of separating a compound from a liquid, which method comprises providing a separation matrix comprising at least one uncharged ligand; providing a liquid wherein the compound to be separated is present in a positively charged state; contacting said matrix with said liquid to adsorb the compound; and removing the liquid. The uncharged ligands possess a quadrupole or dipole moment, allowing for a cation-? interaction between the compound and the ligand. The present invention also encompasses the use of a separation matrix, which comprises an uncharged group that possesses a quadrupole or dipole moment, in said method.Type: GrantFiled: December 17, 2002Date of Patent: March 20, 2012Assignee: GE Healthcare Bio-Sciences ABInventor: Jean-Luc Maloisel
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Patent number: 8129342Abstract: The invention discloses highly purified daptomycin and to pharmaceutical compositions comprising this compound. The invention discloses a method of purifying daptomycin comprising the sequential steps of anion exchange chromatography, hydrophobic interaction chromatography and anion exchange chromatography. The invention also discloses a method of purifying daptomycin by modified buffer enhanced anion exchange chromatography. The invention also discloses an improved method for producing daptomycin by fermentation of Streptomyces roseosporus. The invention also discloses high pressure liquid chromatography methods for analysis of daptomycin purity. The invention also discloses lipopeptide micelles and methods of making the micelles. The invention also discloses methods of using lipopeptide micelles for purifying lipopeptide antibiotics, such as daptomycin. The invention also discloses using lipopeptide micelles therapeutically.Type: GrantFiled: September 22, 2010Date of Patent: March 6, 2012Assignee: Cubist Pharmaceuticals, Inc.Inventors: Thomas J. Kelleher, Jan-Ji Lai, Joseph P. DeCourcey, Paul D. Lynch, Maurizio Zenoni, Auro R. Tagliani
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Publication number: 20120053320Abstract: A nanowire comprising a purified protein filament, such as a pilus, isolated from a bacterium, such as Geobacter sulfurreducens, is provided. Such a purified pilus can contain peptide subunits capable of assembling into the protein filament and establishing an electrical connection with an insoluble electron acceptor. The novel nanowires can be produced via a novel single step. Such nanowires are useful in applications requiring rectifying behavior.Type: ApplicationFiled: August 30, 2011Publication date: March 1, 2012Inventors: Gemma Reguera, Stuart Tessmer, Joshua Veazey, Sanela Lampa-Pastirk
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Patent number: 8124727Abstract: Use of modified metal oxides for the purification and enrichment of negatively charged biomolecules such as peptides, proteins, DNA, RNA, Lipids, carbohydrates, glyco molecules. These metal oxides are modified in such a way that the density of the Lewis acid group is reduced due to modification.Type: GrantFiled: October 22, 2008Date of Patent: February 28, 2012Inventors: Ashok K. Shukla, Mukta M Shukla
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Patent number: 8124722Abstract: There are provided a soluble CD14 antigen which is a novel in vivo protein useful as a marker for diagnosing sepsis and has the following characteristic features 1) to 3): 1) a molecular weight of 13±2 kDa when measured by SDS-PAGE under non-reducing conditions; 2) an amino acid sequence in which the amino acid sequence of SEQ ID NO:1 is present on its N terminal; and 3) ability to specifically bind to an antibody prepared by using a peptide comprising 16 amino acid residues described in SEQ ID NO:2 for the antigen; and a recombinant soluble CD14 fragment.Type: GrantFiled: February 27, 2009Date of Patent: February 28, 2012Assignee: Mochida Pharmaceutical Co., Ltd.Inventors: Shoji Furusako, Kamon Shirakawa, Jiro Hirose
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Publication number: 20120041174Abstract: The present invention relates to methods for the purification of polypeptides using counter current chromatography. In particular the present invention relates to the use of counter current chromatography for the purification of recombinant GLP-1.Type: ApplicationFiled: December 8, 2009Publication date: February 16, 2012Applicant: Novo Nordisk A/SInventor: Søren Søndergaard Frederiksen
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HYDROPHOBIC MONOMERS, HYDROPHOBICALLY-DERIVATIZED SUPPORTS, AND METHODS OF MAKING AND USING THE SAME
Publication number: 20120039920Abstract: A composition is disclosed comprising a hydrophobic monomer having the structure: CH2?CR4C(O)NHC(R1R1)(C(R1R1))nC(O)XR3 wherein n is an integer of 0 or 1; R1 is independently selected from at least one of: a hydrogen atom, alkyls aryls, and alkylaryls, wherein the alkyls, aryls, and alkylaryls have a total of 10 carbon atoms or less; R3 is a hydrophobic group selected from at least one of: alkyls, aryls, alkylaryls and ethers, wherein the alkyls, aryls, alkylaryls and ethers have a total number of carbon atoms ranging from 4 to 30; R4 H or CH3; X is O or NH. In some embodiments the hydrophobic monomer is derived from an amine or an alcohol (HXR3) that has a hydrophilicity index of 25 or less. A polymerizable composition comprising the hydrophobic monomer is disclosed, which optionally may comprise a cross-linking monomer and/or a non-cross-linking monomer.Type: ApplicationFiled: March 19, 2010Publication date: February 16, 2012Inventors: Jerald K. Rasmussen, Cary A. Kipke, James I. Hembre, Peter D. Wickert -
Publication number: 20120009624Abstract: The invention relates to a protein particle comprising chimeric protein having an aggregating part capable of forming or aggregating into a substantially insoluble protein particle when expressed by a cell; and a functional part capable of binding to, or being bound by, a target compound. Affinity matrixes comprising the protein particle are also provided.Type: ApplicationFiled: December 3, 2007Publication date: January 12, 2012Inventors: Jens Sommer-Knudsen, Moreland D. Gibbs
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Publication number: 20120004592Abstract: Tissue solubilizing compositions are provided. The compositions comprise 3-(decyl dimethyl ammonio) propane sulfonate and polyethylene glycol dodecyl ether, such as tetraethylene glycol dodecyl ether. The compositions may be useful to solubilize tissue, including skin, mucosal membrane, and other tissue. The compositions may be further useful to preserve and recover analytes contained within the solubilized skin, mucosal membrane, and other tissue.Type: ApplicationFiled: April 27, 2011Publication date: January 5, 2012Applicant: The Regents of the University of CaliforniaInventors: Samir Mitragotri, Sumit Pallwal, Makoto Ogura, Russell M. Lebovitz
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Publication number: 20120003712Abstract: The present invention provides a method for preparing a site-specific physiologically active polypeptide conjugate in a high yield by treating a physiologically active polypeptide with a non-peptidyl polymer in the presence of an alcohol at a specific pH, which can be desirably employed in the development of long acting formulations of various peptide drugs having high in-vivo activity and markedly prolonged in-blood half-life.Type: ApplicationFiled: March 18, 2010Publication date: January 5, 2012Applicant: HANMI HOLDINGS CO., LTD.Inventors: Dae Hae Song, Jae Hee Shin, Jae Min Lee, Young Kyung Park, Se Chang Kwon, Gwan Sun Lee
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Patent number: 8088626Abstract: The invention relates to the use of compounds as new chiral selectors for separating the optical or enantiomeric isomers of a compound, wherein the chiral selector comprises at least one compound of formula (I): and at least one metal ion, for example Cu2+, Ni2+, Zn2+, Cd2+ or Co2+.Type: GrantFiled: March 13, 2008Date of Patent: January 3, 2012Assignee: University Joseph Fourier (Grenoble 1)Inventors: Eric Peyrin, Jean-Luc Decout, Corrine Ravelet, Isabelle Henriette Baussanne
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Publication number: 20110313131Abstract: The invention comprises a process for the purification of a GLP-1 peptide analogue applying reversed phase high performance liquid chromatography (RP-HPLC).Type: ApplicationFiled: June 13, 2011Publication date: December 22, 2011Inventors: Christelle Carl, Michael Rothe, Christian Saladin, Daniel Strub, Francis Vix
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Patent number: 8071714Abstract: The invention relates to a process for separating and/or purifying organic compounds susceptible to crystallization by means of crystallizing and dissolving comprising the following steps: a) depositing the composition in the head of a separation and/or crystallization column; b) crystallizing by means of a cooling gradient; c) pumping the solvent at optimal flow rate Fc; d) entraining the components while they are not crystallized to the end of the column; e) stopping the pumping of the solvent until reaching the lowest temperature of the interval established by the cooling gradient; f) heating the column; g) beginning new pumping by means of applying flow rate Fe; h) collecting the eluates; and i) detecting by means of detectors.Type: GrantFiled: March 15, 2006Date of Patent: December 6, 2011Assignee: Polymer Characterization, S.A.Inventor: Benjamín Monrabal Bas
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Patent number: 8058238Abstract: The invention discloses highly purified daptomycin and to pharmaceutical compositions comprising this compound. The invention discloses a method of purifying daptomycin comprising the sequential steps of anion exchange chromatography, hydrophobic interaction chromatography and anion exchange chromatography. The invention also discloses a method of purifying daptomycin by modified buffer enhanced anion exchange chromatography. The invention also discloses an improved method for producing daptomycin by fermentation of Streptomyces roseosporus. The invention also discloses high pressure liquid chromatography methods for analysis of daptomycin purity. The invention also discloses lipopeptide micelles and methods of making the micelles. The invention also discloses methods of using lipopeptide micelles for purifying lipopeptide antibiotics, such as daptomycin. The invention also discloses using lipopeptide micelles therapeutically.Type: GrantFiled: April 24, 2007Date of Patent: November 15, 2011Assignee: Cubist Pharmaceuticals, Inc.Inventors: Thomas Kelleher, Jan-Ji Lai, Joseph P. DeCourcey, Paul Lynch, Maurizio Zenoni, Auro Tagliani
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Patent number: 8058390Abstract: The present invention includes crystallized HDM2 peptides as well as descriptions of the X-ray diffraction patterns of the crystals. The diffraction patterns allow the three dimensional structure of HDM2 to be determined at atomic resolution so that ligand binding sites on HDM2 can be identified and the interactions of ligands with HDM2 amino acid residues can be modeled. Models prepared using such maps permit the design of ligands which can function as active agents which include, but are not limited to, those that function as inhibitors of MDM2 and HDM2 oncoproteins.Type: GrantFiled: April 23, 2010Date of Patent: November 15, 2011Assignee: Ortho-McNeil Pharmaceutical, Inc.Inventors: Carsten Schubert, Bruce Grasberger, Diane Maguire, Ingrid Deckman, John Spurlino
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Patent number: 8058235Abstract: Methods of making copolymers are described.Type: GrantFiled: July 20, 2011Date of Patent: November 15, 2011Assignee: Momenta Pharmaceuticals, Inc.Inventors: Claire Coleman, John Schaeck, Alicia Thompson
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Publication number: 20110263446Abstract: A kit for separating and/or characterizing at least one molecule A of interest, the kit including: (i) a compound having the general formula: B—(R)n—Z in which: B represents a hydrogen atom or a detectable labeling entity; R represents a C1-C10000 hydrocarbon unit which may be polymeric or non-polymeric and optionally incorporates one or more heteroatoms, chosen from N, O, S, Br, Cl, F, P, B, Si and/or one or more metals; n represents 0 or 1, with n being equal to 1 when B represents a hydrogen atom; and Z represents a functional group capable of reacting in a click chemistry reaction in order to form a linking function Lclick; and (ii) a molecularly imprinted polymer dedicated to the molecular recognition of at least said linking function Lclick.Type: ApplicationFiled: September 9, 2009Publication date: October 27, 2011Applicant: POLYINTELLInventors: Alexandra Le Foll, Vincent Levacher, Celine Perollier, Sami Bayoudh, Kaynoush Naraghi
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Publication number: 20110245461Abstract: The present disclosure relates to methods for separating or isolating a peptide using reverse phase chromatography. The disclosure also relates to methods for calculating or determining the slope S of a peptide, wherein S is defined according to the Linear-Solvent-Strength equation log k=log k0?S*?. Also provided are a set of peptides with known S values suitable for use in the described methods.Type: ApplicationFiled: March 28, 2011Publication date: October 6, 2011Inventors: Oleg V. Krokhin, Vic Spicer
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Publication number: 20110207196Abstract: The present invention relates to a depth filter layer with inorganic layer double hydroxide, a method for production thereof, a filtration device containing this, a method for the separation of at least one contaminant from at least one target product in a liquid medium by means of at least one upstream depth filter layer and the use of the depth filter layer for removal of contaminants. The depth filter layer selectively retains contaminants such as nucleic acids, while target proteins of biotechnological processes are transmissible into the filtrate.Type: ApplicationFiled: August 11, 2009Publication date: August 25, 2011Applicant: SARTORIUS STEDIM IOTECH GMBHInventors: Katrin Koehler, Axel Thiefes, Klaus Tarrach
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Increased dynamic binding capacity in ion exchange chromatography by addition of polyethylene glycol
Patent number: 7998705Abstract: The present invention generally relates to novel processes for protein purification in high salt solutions such as cell culture broth by increasing the dynamic binding capacity of a resin with the addition of polyethylene glycol.Type: GrantFiled: November 12, 2002Date of Patent: August 16, 2011Assignee: FUJIFILM Diosynth Biotechnologies U.S.A., IncInventors: Min Wan, Mark David Chavez, Jeffrey Lee Schrimsher -
Patent number: 7994128Abstract: The invention relates to an isolated, synthetic or recombinant ?-conotoxin peptide having the ability to inhibit a neuronal amine transporter, nucleic acid molecules encoding all or part of such peptides, antibodies to such peptides and uses and methods of treatment involving them.Type: GrantFiled: November 2, 2007Date of Patent: August 9, 2011Assignee: Xenome LimitedInventors: Richard J. Lewis, Paul F. Alewood, Iain A. Sharp
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Publication number: 20110184146Abstract: A polypeptide is isolated from Turmeric (Curcuma longa Linn) having molecular weight of about 8,000 Daltons. The polypeptide is highly water-soluble and absolutely non-toxic antioxidant, which is isolated using boiling water extraction. The polypeptide is an excellent antioxidant working at low concentration (of about 80 nM) to quench lipid peroxidation up to 90%. The polypeptide is highly effective against oxidative stress related diseases like arthritis, atherosclerosis, cardiovascular diseases, neurodegenerative diseases, cancer, cataract, malaria, bacterial and fungal infectious diseases.Type: ApplicationFiled: January 18, 2011Publication date: July 28, 2011Inventor: Leela SRINIVAS
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Patent number: 7977119Abstract: Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.Type: GrantFiled: December 8, 2004Date of Patent: July 12, 2011Assignee: Agilent Technologies, Inc.Inventors: Mel N. Kronick, Eric M. Leproust
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Patent number: 7977456Abstract: A simple, efficient apparatus and method for separating layers of immiscible or partially miscible liquids useful in methods of high-throughput combinatorial organic synthesis or parallel extraction of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis or purification (extraction). In a preferred embodiment, an apparatus and method for separating layers of immiscible or partially miscible liquids compatible with microtiter plate type array(s) of reaction vessels is disclosed. Another application of centrifugation based liquid removal was found for washing the plates in biological assays or synthesis on modified substrates.Type: GrantFiled: May 3, 2004Date of Patent: July 12, 2011Assignee: Illumina, Inc.Inventor: Michal Lebl
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Publication number: 20110151538Abstract: The present invention is directed to truncated dockerin polypeptides, recombinant polypeptides and affinity systems comprising the truncated dockerin polypeptide, methods of generating same, and methods of use thereof to purify, isolate, and detect molecules of interest.Type: ApplicationFiled: August 9, 2009Publication date: June 23, 2011Applicants: RAMOT AT TEL-AVIV UNIVERSITY LTD., YEDA RESEARCH AND DEVELOPMENT CO. LTD. AT THE WEIZMANN INSTITUTE OF SCIENCEInventors: Edward A. Bayer, Alon Karpol, Raphael Lamed
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Patent number: 7960340Abstract: The present invention relates generally to a molecular framework having a cyclic structure. More particularly, the present invention provides cyclic proteins and derivatives thereof in which particular turns and other elements of the molecular structure are held in defined orientations with respect to each other. The cyclic proteins of the present invention provide a molecular framework for the introduction of particular amino acids or heterologous amino acid sequences to facilitate the presentation of biological activities associated with these heterologous amino acid sequences. The molecular framework of the present invention may be naturally cyclic or may be a cyclized derivative of a linear molecular or may be a linear derivative of a cyclized molecule. The present invention contemplates the use of the molecular framework with or without particular amino acids inserted or substituted thereon for the treatment of or prophylaxis of disease conditions in animals, mammals (including humans) and plants.Type: GrantFiled: March 1, 2010Date of Patent: June 14, 2011Assignee: The University of QueenslandInventors: David J. Craik, Norelle Lee Daly, Clement Waim-Kunduane Waine
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Patent number: 7960344Abstract: The invention relates to glucagon-related peptides and their use for the prevention or treatment of disorders involving the large intestine. In particular, it has now been demonstrated that GLP-2 and peptidic agonists of GLP-2 can cause proliferation of the tissue of large intestine. Thus, the invention provides methods of proliferating the large intestine in a subject in need thereof. Further, the methods of the invention are useful to treat or prevent inflammatory conditions of the large intestine, including inflammatory bowel diseases.Type: GrantFiled: October 16, 2009Date of Patent: June 14, 2011Assignee: 1149336 Ontario, Inc.Inventor: Daniel J. Drucker
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Publication number: 20110130542Abstract: Method for removing and selective separating peptides and proteins from a solution by controlled crystallization.Type: ApplicationFiled: June 16, 2009Publication date: June 2, 2011Applicant: BAYER TECHNOLOGY SERVICES GMBHInventors: Joerg Kauling, Dirk Havekost, Hans-Jürgen Henzler
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Patent number: 7951913Abstract: The invention is related to the method of polymyxine B recovery from fermentation broth for the purpose of pure substance recovery. The invention mentioned above is obtained by using the method of polymyxine B recovery from fermentation broth according invention, which abstract is characterized by, that the filtrate is obtained from fermentation broth, its pH and color is adjusted and purified on non-ionogenic synthetic adsorbents of polystyrene type with specific surface 500 till 1000 m2·g?1 and fabric size 3 till 30 nm. The polymyxine B is eluated from adsorbent by aqueous solution of organic solvent. The polymyxine B base is coagulated from eluate after adjustment of pH to alcaline area. The polymyxine B base is converted by mineral acid to polymyxine B salt solution, from which is obtained crystal substance by drying.Type: GrantFiled: May 11, 2007Date of Patent: May 31, 2011Assignee: Biotika A.S.Inventors: Ivan Varga, Mária Bobálová, Eva Michalková, Mária Jakubcová