Chromatography Patents (Class 73/61.52)
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Patent number: 8728828Abstract: The present invention relates to a separation matrix comprised of porous particles to which antibody-binding protein ligands have been immobilized, wherein the ligand density is in the range of 5.0-10 mg/ml; the gel phase distribution coefficient of the particles expressed as Kav for a dextran of size 110 kDa is above 0.65 and the median particle diameter is between 65-84 ?m. The carbohydrate material is preferably highly cross-linked agarose.Type: GrantFiled: December 22, 2005Date of Patent: May 20, 2014Assignee: GE Healthcare Bio-Sciences ABInventors: Hans Berg, Hans Johansson, Gunnar Malmquist, Per-Mikael Aberg
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Publication number: 20140130580Abstract: A fluidic connection system may comprise a port which includes a capillary tube receptacle with an end seal face and a threaded wall. The fluidic connection system may also comprise a capillary tube that includes a front end face for sealingly abutting against the end seal face of the port. On the capillary tube, a flange may be affixed at a distance from the front end face of the capillary tube. The fluidic connection system may further comprise a fitting nut that includes an axial hole to allow the capillary tube to pass therethrough and a matching threaded wall corresponding to the threaded wall of the port. The fitting nut may be slidably coupled with the capillary tube behind the flange. The distance at which the flange is affixed may be predetermined such that when the flange is pushed forward by the fitting nut, the front end face of the capillary tube abuts directly against the end seal face of the port.Type: ApplicationFiled: November 12, 2012Publication date: May 15, 2014Inventors: Michael John MCADAMS, Milton LIU, George N. PROPER
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Patent number: 8721892Abstract: Systems and methods for monitoring analytes in real time using integrated chromatography systems and devices. Integrated microfluidic liquid chromatography devices and systems include multiple separation columns integrated into a single substrate. Using such a device, parallel analysis of multiple samples can be performed simultaneously and/or sequential analysis of a single sample can be performed simultaneously on a single chip or substrate. The devices and systems are well suited for use in high pressure liquid chromatography (HPLC) applications. HPLC chips and devices including embedded parylene channels can be fabricated using a single mask process.Type: GrantFiled: October 28, 2013Date of Patent: May 13, 2014Assignee: California Institute of TechnologyInventors: Chi-Yuan Shih, Yu-Chong Tai, Jun Xie, Darron K. Young, Po-Jui Chen
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Publication number: 20140116118Abstract: Indole-3-propionic acid as a marker and for treatment for Huntington Disease.Type: ApplicationFiled: November 27, 2013Publication date: May 1, 2014Applicant: COUNTERPOINT HEALTH SOLUTIONS, INC.Inventor: Wayne R. Matson
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Patent number: 8702983Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.Type: GrantFiled: June 4, 2012Date of Patent: April 22, 2014Assignee: GE Healthcare Bio-Sciences ABInventor: Klaus Gebauer
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Patent number: 8696901Abstract: The present invention relates to a polymeric porous material characterized in that: the porous material has a bimodal pore size distribution attributable to macropores having a pore size of at least 50 nm and mesopores having a pore size of from 2 nm to less than 50 nm, the proportion of the specific surface area of the macropores to the specific surface area of all pores of the porous material is at least 10%, and the porous material is produced by (1) polymerizing a monomer in the presence of a polymerization initiator using as a porogen a solution obtained by dissolving a polymer having a weight-average molecular weight of at least 100,000 and a molecular weight distribution Mw/Mn of not more than 1.5 in a good solvent for the monomer, and (2) removing the porogen from the resultant product.Type: GrantFiled: May 9, 2006Date of Patent: April 15, 2014Assignees: National University Corporation Kyoto Institute of Technology, Emaus Kyoto, Inc.Inventors: Ken Hosoya, Hiroshi Aoki, Norio Ishizuka, Katsuya Yamamoto
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Publication number: 20140096596Abstract: The invention provides novel porous materials that are useful in chromatographic processes, e.g., solid phase extraction, and that provide a number of advantages. Such advantages include superior wetting characteristics, selective capture of analytes of interest, and non-retention of interfering analytes. The invention advantageously provides novel porous materials having a large percentage of larger pores (i.e. wide pores). The invention advantageously provides novel porous materials that overcome the problems of SPE of biological samples.Type: ApplicationFiled: May 18, 2012Publication date: April 10, 2014Applicant: WATERS TECHNOLOGIES CORPORATIONInventors: Darryl W. Brousmiche, Kevin D. Wyndham
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Publication number: 20140090452Abstract: A high performance liquid chromatography method to routinely and reproducibly detect and quantitate metal complexes is provided. The metal complexes used in the method of the invention can be different metal complexes, or they can be stereoisomers of the same metal complexes. The high performance liquid chromatography method of the present invention is suitable for the separation of diastereomers of the same metal complexes. Also provided is a chiral high performance liquid chromatography method to separate enantiomers of metal complexes. Superoxide dismutase mimetic compounds are also provided.Type: ApplicationFiled: April 24, 2013Publication date: April 3, 2014Applicant: GALERA THERAPEUTICS, LLCInventors: Urszula J. Slomczynska, Bobby N. Trawick, Dennis P. Riley, Arati Naik
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Publication number: 20140090453Abstract: The invention provides an apparatus for polymer chromatography, comprising at least one column that comprises a first stationary phase comprising one of the following: A) a material comprising at least one non-carbon atom, excluding glass or a metal, selected from molybdenum sulfide MoS2, tungsten sulfide WS2, silicon carbide SiC, boron nitride BN, or combinations thereof, or B) glass, or a metal, or combinations thereof, and a material comprising at least one non-carbon atom selected from molybdenum sulfide MoS2, tungsten sulfide WS2, silicon carbide SiC, boron nitride BN, or combinations thereof. The invention also provides a method for polymer chromatography, comprising introducing a solution, comprising a polymer, into a liquid flowing through a first stationary phase, and wherein the first stationary phase comprises one of foregoing materials (A) or (B).Type: ApplicationFiled: June 1, 2012Publication date: April 3, 2014Applicant: DOW GLOBAL TECHNOLOGIES LLCInventors: Rongjuan Cong, Charles M. Cheatham, Al Parrott, Wallace W. Yau, Lonnie G. Hazlitt, Zhe Zhou, Alexander W. Degroot, Matthew D. Miller
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Patent number: 8679346Abstract: An enantiomeric isomer separating agent includes a polysaccharide derivative supported by particles of a support by chemical bonding and has a concentration of eluted component (in terms of mass proportion), as determined through a liquid passing test of 20 ppm or lower.Type: GrantFiled: August 24, 2011Date of Patent: March 25, 2014Assignee: Daicel CorporationInventor: Yasuhiro Kagamihara
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Patent number: 8658428Abstract: In at least one embodiment, the invention is directed to a method of determining the nitrogen content in nitrocellulose by High Performance Liquid Chromatography (HPLC). It is within the scope of the invention for the sample of nitrocellulose to be unstable nitrocellulose, unrefined nitrocellulose, or refined nitrocellulose. The sample of nitrocellulose can wet or dry. A sample of nitrocellulose that is wet can be in acid or in water. Before HPLC, the sample is dissolved in a suitable solvent. The nitrogen content of the sample is determined by comparing the retention time of the sample to a graph of retention time to amount of nitrogen due to the linear correlation of retention time and percent nitrogen substitution.Type: GrantFiled: July 1, 2011Date of Patent: February 25, 2014Assignee: Alliant Techsystems Inc.Inventor: Michael T. Combs
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Publication number: 20140047905Abstract: An electrospray emitter assembly (4) for an interface device (1) used to connect the output of a liquid chromatograph to the inlet of an electrospray ion source of a mass spectrometer (10). The device (1) incorporates an electrospray emitter assembly (4) that includes an emitter needle (40), a sheath (6) and a housing (5) with an attachment means in the form of a flange (55) for attachment to a mass spectrometer (10). The sheath (6) is arranged to shield the electrospray emitter needle (40) when the assembly is not attached to a mass spectrometer (10). The assembly (4) is operable to move the sheath (6) thereby to reveal, in use, the electrospray emitter needle (40) when the assembly (4) is attached to the mass spectrometer (10).Type: ApplicationFiled: March 5, 2012Publication date: February 20, 2014Applicant: WATERS TECHNOLOGIES CORPORATIONInventors: Michael J. Tomany, Paul Rainville, James P. Murphy
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Patent number: 8650936Abstract: A liquid chromatography device includes a sample inlet valve to which a sample to be analyzed is introduced, and a trap valve fluid-communicating with a solid phase extraction column and a reverse phase liquid chromatography column. The device further includes a solvent dividing unit dividing flow of a solvent discharged from the sample inlet valve into the first solvent inlet port and the second solvent inlet port of the trap valve. The liquid chromatography device improves resolution and increases reproducibility by allowing the sample injecting direction and the sample eluting direction to be opposite to each other.Type: GrantFiled: July 11, 2011Date of Patent: February 18, 2014Assignee: Korea University Research and Business FoundationInventors: Sang-Won Lee, Min-Sik Kim, Seok-Won Hyung
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Patent number: 8641882Abstract: Described is a capillary column cartridge. The cartridge can be used to perform separations according to various techniques such as capillary gas chromatography, capillary electrophoresis and capillary liquid chromatography. The cartridge includes a capillary column secured in a cartridge body. The capillary column includes an inlet port and an outlet port that, in some embodiments, are disposed on a planar surface of the body. When the body is engaged to a separation system module, the inlet port is aligned to receive a sample to be separated and the outlet port is aligned to provide the separated sample to the separation system module. The path of the capillary through the body has a non-planar path shape such as a coil shape. Consequently, longer column lengths can be accommodated, leading to an improvement in separation resolution. The body can include a material having a high thermal conductivity to achieve improved thermal performance.Type: GrantFiled: June 20, 2011Date of Patent: February 4, 2014Assignee: Waters Technologies CorporationInventors: Joseph A. Jarrell, Keith Edward Fadgen
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Publication number: 20140020447Abstract: In the method of latent print analysis, wherein the improvement comprises using an ultra-fine, nanoporous carbon material as an adsorptive powder to identify trace chemicals found within oils of a print.Type: ApplicationFiled: March 14, 2013Publication date: January 23, 2014Applicant: BAE Systems Information and Electronic Systems Integration Inc.Inventor: BAE Systems Information and Electonic Systems Integration Inc.
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Publication number: 20140005233Abstract: A composition having clevidipine as an active ingredient is described. The composition includes clevidipine as an active ingredient and an amount of the impurity H168/79 that is no greater than about 1.5%, or where the ratio between clevidipine and H168/79 is equal or above 60 to 1.Type: ApplicationFiled: June 17, 2013Publication date: January 2, 2014Applicant: The Medicines CompanyInventors: Rajeshwar Motheram, Gopal Krishna, Min Ding, Keith Flood, Kornepati Ramakrishna
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Patent number: 8608961Abstract: The present invention is directed to an affinity chromatography column comprising the antithrombin III (ATIII) protein bound to a solid support, characterized in that: a. the ATIII protein is the wild-type protein or a variant thereof, b. the ATIII protein has been first activated by incubation with an unmodified low-molecular-weight heparin (LMWH) rich in active species, and c. the ATIII protein is covalently bound to a resin in a ratio of less than approximately 2 mg of protein per ml of hydrated resin. The invention is also directed to the use of the aforesaid column for purifying species having an affinity for ATIII in a sample comprising species having affinity and not having affinity for ATIII.Type: GrantFiled: January 28, 2008Date of Patent: December 17, 2013Assignee: Aventis Pharma S.A.Inventors: Pierre Mourier, Gerald Perret
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Publication number: 20130319086Abstract: The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier.Type: ApplicationFiled: January 25, 2013Publication date: December 5, 2013Applicant: Waters Technologies CorporationInventors: Kevin Daniel Wyndham, Pamela C. Iraneta, Thomas H. Walter
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Patent number: 8597571Abstract: Electrolytic eluent recycle systems for ion chromatography using a multi-channel electrolytic ion exchange device which integrates suppression, eluent generation, and eluent recycle. The systems recycle the eluent into the analytical system without passing the eluent through the electrode chambers. Also, such systems with a channel for electrolytic removal of ions from the suppression effluent before recycle.Type: GrantFiled: January 27, 2009Date of Patent: December 3, 2013Assignee: Dionex CorporationInventor: John M. Riviello
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Publication number: 20130306854Abstract: Disclosed herein is a method of preparing a protein sample for mass spectroscopy. The method includes separating proteins of the sample on an electrophoresis gel; contacting the proteins with a halo-substituted organic compound; exposing the gel to UV light; detecting fluorescence emitted from the electrophoresis gel; excising at least one portion of the electrophoresis gel based upon the detected fluorescence, wherein said at least one portion contains proteins of the protein sample; and subjecting proteins from the at least one portion to mass spectroscopy. Using this method, more proteins can be identified by GeLC-MS than when the electrophoresis gel is treated with a protein stain or subjected to the gel handling steps accompanying such treatment.Type: ApplicationFiled: May 17, 2013Publication date: November 21, 2013Inventor: Christopher Belisle
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Patent number: 8574432Abstract: Systems and methods for monitoring analytes in real time using integrated chromatography systems and devices. Integrated microfluidic liquid chromatography devices and systems include multiple separation columns integrated into a single substrate. Using such a device, parallel analysis of multiple samples can be performed simultaneously and/or sequential analysis of a single sample can be performed simultaneously on a single chip or substrate. The devices and systems are well suited for use in high pressure liquid chromatography (HPLC) applications. HPLC chips and devices including embedded parylene channels can be fabricated using a single mask process.Type: GrantFiled: April 14, 2006Date of Patent: November 5, 2013Assignee: California Institute of TechnologyInventors: Chi-yuan Shih, Yu-Chong Tai, Jun Xie, Darron K. Young, Po-Jui Chen
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Patent number: 8569070Abstract: A fitting assembly having a nut, a ferrule, and a ferrule tip that may be assembled by an operator. The fitting assembly includes a nut with first and second ends, with the second end adapted to receive the first end of a ferrule, and a ferrule tip with a first end having an externally tapered portion adapted to abut the second end of the ferrule and a second end adapted to be received in a component or fitting of a liquid chromatography system. The nut, ferrule and ferrule tip of the fitting assembly have passageways therethrough for receiving and removably holding tubing.Type: GrantFiled: July 16, 2010Date of Patent: October 29, 2013Assignee: IDEX Health & Science LLCInventors: Scott J. Ellis, Eric Beemer, Nathaniel Nienhuis, Craig W. Graham, Troy N. Sanders
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Publication number: 20130260397Abstract: The present invention relates a method for the detection of at least one mycotoxin contamination from bulk ware, an apparatus for taking a sample of particles from bulk ware as well as a method for taking particles from bulk ware using the apparatus according to the invention and the use of the apparatus according to the invention.Type: ApplicationFiled: September 7, 2011Publication date: October 3, 2013Applicant: EUROFINS WEJ CONTAMINANTS GMBHInventors: Scarlett Biselli, Ngnintendem James Nkengfack
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Patent number: 8544350Abstract: This invention provides a sample injector for injecting a fixed amount of a sample into a mobile phase medium and discharging the mobile phase medium with the sample. The sample injector includes a first member having a medium passage for supplying the mobile phase medium and a sample passage for supplying the sample and a second member having a discharge passage for discharging the mobile phase medium with the sample to an outside of the injector. The second member is provided in contact with the first member. The first member and the second member are configured to move to a sample charging position and a sample injection position. The sample charging position is for the sample chamber to connect with the sample passage. The sample injection position is for the sample chamber to connect with the medium passage and the discharge passage.Type: GrantFiled: March 4, 2011Date of Patent: October 1, 2013Assignee: CKD CorporationInventors: Hiroshi Itafuji, Masayuki Kouketsu
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Publication number: 20130251738Abstract: There is provided a method of identifying a protective Leptospira vaccine composition, comprising determining that the composition contains a protective concentration of a Lipl32 epitope polypeptide, for example, of a polypeptide comprising SEQ ID NO:13 or an antigenic variant or portion thereof. There is also provided a vaccine composition comprising a protective concentration of at least one Lipl32 epitope polypeptide having up to 250 amino acids and comprising SEQ ID NO:13 or an antigenic variant or portion thereof. There is further provided a method of identifying an immunogenic element in a vaccine composition comprising submitting the composition to a two-dimensional liquid chromatography mass spectrometry (2D LC/MS) process.Type: ApplicationFiled: September 13, 2011Publication date: September 26, 2013Applicant: The Secretary of State for Environment, Food & Rural AffairsInventors: Phillip C. Humphryes, Nicholas G. Coldham
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Publication number: 20130247654Abstract: A method for evaluating a sensory stimulus component in a test component with a taste-analyzing liquid chromatograph system. The method includes: measuring the test component with the taste-analyzing liquid chromatograph system to obtain taste evaluation data; and evaluating a persistence of a sensory stimulus intensity of the test component from a continuous intensity change of the test component using the taste evaluation data as an index.Type: ApplicationFiled: March 20, 2013Publication date: September 26, 2013Applicant: TAKASAGO INTERNATIONAL CORPORATIONInventors: Daisuke SUGIMOTO, Yoshihiro YAGUCHI, Kyoko ZAIZEN, Toshinori SAIMA
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Publication number: 20130253187Abstract: The present invention relates to a process for determining the suitability for distribution of a batch of rivaroxaban or of a pharmaceutical composition thereof. In particular, it also relates to two impurities of rivaroxaban, to their use as reference markers to determine the purity of a sample of rivaroxaban or a composition thereof, to analytical methods for determining the purity of a sample of rivaroxaban or a composition thereof and to a process of preparing rivaroxaban or pharmaceutical compositions thereof which are free or substantially free of such impurities.Type: ApplicationFiled: September 14, 2010Publication date: September 26, 2013Applicant: Medichem, S.A.Inventors: Bernardino Mangion, Ernesto Duran Lopez
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Patent number: 8541243Abstract: The invention provides a method for measuring the levels of 5-methyltetrahydrofolate (5-MTHF), tetrahydrofolate (THF), and 5,10-MTHF in a biological sample. The method includes employing an isotope dilution liquid chromatography-multiple reaction monitoring/mass spectrometry (LC-MRM/MS) methodology.Type: GrantFiled: July 8, 2009Date of Patent: September 24, 2013Assignee: The Trustees of the University of PennsylvaniaInventors: Alexander Steven Whitehead, Ian A. Blair
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Publication number: 20130233060Abstract: A solvent delivery device comprises a solvent delivery pump and a flow path switching valve. The flow path switching valve is connected to downstream ends of mobile phase sending flow paths and the solvent delivery pump is to be connected to one of the mobile phase sending flow paths by switching the flow path switching valve. At least one of the mobile phase sending flow paths includes a buffer solution sending flow path, and the buffer solution sending flow path includes a buffer solution storage section, a cleaning solution storage section, and a mobile phase switching valve for switching connection of the flow path switching valve to one of the storage sections.Type: ApplicationFiled: March 4, 2013Publication date: September 12, 2013Applicant: SHIMADZU CORPORATIONInventors: Keisuke Ogawa, Yoshiaki Maeda
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Publication number: 20130233053Abstract: A torque limited fitting is provided having a head and a body, which in certain embodiments may be assembled by an operator. The body of the torque limited fitting has a passageway therethrough for receiving and removably holding tubing. The torque limited fitting may be adapted for use with a flat bottom port, such as in an analytical instrument, like liquid chromatography, gas chromatography, ion chromatography, or in in vitro diagnostic systems.Type: ApplicationFiled: November 2, 2012Publication date: September 12, 2013Applicant: IDEX Health & Science LLCInventors: Eric Beemer, Craig Graham, Mark Hahn
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Patent number: 8522603Abstract: Disclosed is an improved post-column fluorimetric determination-boric acid complex anion exchange method which allows analysis of mannose-6-phosphate. The disclosed method is a method for separation analysis of reducing sugars using column chromatography, comprising loading a sample onto an anion exchange column, washing the column by allowing to flow a sufficient volume of a first mobile phase consisting of an aqueous solution of a predetermined concentration of boric acid containing a predetermined concentration of a water-soluble inorganic salt through the column, supplying a second mobile phase with an elevated concentration of the salt to elute the reducing sugars, adding to the eluate a basic amino acid, heating, and continuously measuring and recording the intensity of fluorescent light emitted under irradiation with excitation light.Type: GrantFiled: March 12, 2010Date of Patent: September 3, 2013Assignee: JCR Pharmaceuticals Co., Ltd.Inventor: Tetsuo Yokoyama
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Publication number: 20130219999Abstract: A liquid chromatography device comprises one or more liquid reservoirs (3) for a liquid medium, a sample reservoir for a sample to be analysed and a chromatography column (4) in fluid communication with the liquid reservoir (3) and the sample reservoir (5). The device further comprises a gas reservoir (1) for containing a volume of gas under pressure to force liquid from the liquid reservoir (3) through the chromatography column (4), in use.Type: ApplicationFiled: June 27, 2011Publication date: August 29, 2013Applicant: IMPERIAL INNOVATIONS LTDInventors: Duncan Robert Casey, Joseph John Kaplinsky, Ali Salehi-Reyhani
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Publication number: 20130210865Abstract: The present invention provides dimethyl sulphoxide solvate of 4-(4-{3-[4-chloro-3-(trifluoromethyl)phenyl]ureido}phenoxy)-N2-methylpyridine-2-carboxamide, process for its preparation, pharmaceutical composition comprising it and its use for the treatment of cancer. The present invention also provides a novel HPLC method for the identification, quantification and isolation of related substances of sorafenib.Type: ApplicationFiled: January 28, 2011Publication date: August 15, 2013Applicant: RANBAXY LABORATORIES LIMITEDInventors: Jagdev Singh Jaryal, Swargam Sathyanarayana, Rajesh Kumar Thaper, Mohan Prasad, Sachin Deorao Zade, Ajay Shankar Sinch
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Publication number: 20130205877Abstract: Aspects of the present application relate to molecular weight markers of glatiramer acetate for accurate determination of the average molecular weight of glatiramer acetate.Type: ApplicationFiled: July 28, 2011Publication date: August 15, 2013Applicants: DR. REDDY'S LABORATORIES, INC., DR. REDDY'S LABORATORIES LTD.Inventors: Santhanakrishnan Srinivasan, Karthik Ramasamy, Chakravarthula Kalyan Narasimham Nallam, Yagna Kiran Kumar Komaravolu, Sudheer Reddy Kallam, Bala Harsha Vardhan Ganji, Ravindra Chary Bathoju, Basanthi Devi
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Patent number: 8507260Abstract: Disclosed is a lateral flow capillary device and uses thereof comprising a unipath bibulous capillary flow matrix and at least two reservoirs each in fluid communication with the capillary flow matrix wherein a reservoir contacts the capillary flow matrix through a passage having a rim pressing the matrix. The pressure that the rim applies on the matrix prevents leakage of liquids out of the capillary flow matrix at the reservoir/ matrix interface, allowing accurate sequential draining of liquid from the reservoirs.Type: GrantFiled: June 2, 2010Date of Patent: August 13, 2013Assignee: Realbio Technologies, Ltd.Inventors: Sara Alajem, Avraham Reinhartz
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Patent number: 8506801Abstract: A device for capillary chromatography comprising at least one first receiving space arranged in a circuit board and of a form such that the first receiving space is suitable for at least partially receiving a coiled part of a capillary separation column. Also a method for manufacturing such a device. Using such a device and method the greatest possible advantage can be gained from both miniaturized and integrated gas chromatographs (minimal dead volumes; integration options; small thermal masses and rapid temperature regulation; low cost price; small dimensions, weight and energy consumption; portable and flexible in use) and from the use of a usual capillary separation column (very good separation; high precision and reproducibility). The capillary separation column can herein be heated uniformly, rapidly and accurately.Type: GrantFiled: February 1, 2008Date of Patent: August 13, 2013Assignee: Concept to Volume B.V.Inventors: Gert-Jan Burger, Anne Freerk De Jager, Harm Jan Van Weerden
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Publication number: 20130199277Abstract: An object of the present invention is to provide a hemoglobin S analysis method, a hemoglobin A2 analysis method, and a hemoglobin A0 analysis method which enable even highly retentive hemoglobin S, hemoglobin A2 and hemoglobin A0 to be separated in sharp, highly symmetrical peaks by means of cation-exchange high-performance liquid chromatography. The present invention relates to a method for analyzing hemoglobin S by cation-exchange high-performance liquid chromatography, the method including utilizing an eluent that contains an azide or a cyanide at a concentration of 0.1 to 50 mmol/L and has a pH of 6.80 to 7.50.Type: ApplicationFiled: February 2, 2011Publication date: August 8, 2013Inventors: Hiroaki Taira, Oka Takayuki
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Patent number: 8496819Abstract: Both a column having high-pressure resistance and capable of performing high-speed separation and analysis even with a small flow rate and a liquid chromatograph apparatus using the column are provided. A separation column according to the present invention has a monolithic rod being arranged in the center, being made of a porous material, and having a circular cross section, a filler layer arranged such that it encircles a circumferential surface of the monolithic rod, and a cylindrical support medium arranged outside the filler layer. The filler layer is formed by filling a tube-like gap between the monolithic rod and the support medium with particles or beads.Type: GrantFiled: June 26, 2008Date of Patent: July 30, 2013Assignee: Hitachi High-Technologies CorporationInventors: Katsutoshi Shimizu, Masahito Ito, Kimihiko Ishii, Yoshihiro Nagaoka, Kisaburo Deguchi, Takefumi Yokokura
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Patent number: 8496818Abstract: A high pressure liquid chromatography (HPLC) capillary column device, system and method for processing a HPLC sample with a cartridge housing a packed capillary column; at least one inlet connection to the capillary column for a sample fluid; and at least one outlet connection from the capillary column for the sample fluid. The outlet connection is able to accommodate either a spray tip for atomizing the sample fluid or a transport tube for transporting the sample fluid from a spray tip column to a spray tip. Inlet connections enable supply of electrical power to the capillary column through electrical connections disposed within the cartridge housing; and gas for evaporating the sample liquid is supplied to at least one outlet connection from the capillary column for the sample fluid through a gas supply line within the cartridge housing. The temperature of the sample liquid can be controlled through a heat connection.Type: GrantFiled: October 7, 2004Date of Patent: July 30, 2013Assignee: Waters Technologies CorporationInventors: Christopher C. Benevides, Robert Albert Collamati, Bruce J. Compton, Theodore Dourdeville
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Publication number: 20130172430Abstract: Methods of prognosis and monitoring of breast cancer include determining the level of one or more of the markers comprising asymmetric dimethyl arginine (ADMA), beta-hydroxybutyrate (BHB) and microRNA-31 (miR-31) in patient samples. An increased level is correlated with poor prognosis. Breast cancer is treated by administration of one or more inhibitors of ADMA and/or BHB.Type: ApplicationFiled: December 8, 2011Publication date: July 4, 2013Inventors: Michael P. Lisanti, Federica Sotgia
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Patent number: 8476491Abstract: The invention relates to a method of utilizing the pts gene and RNA interference of the ads gene to increase patchouli alcohol content in Artemisia annua L. plants. Using transgenic Artemisia annua L. plants, the method of the invention consistently increases the patchouli alcohol content in those plants, thus laying down a solid foundation for large-scale production of patchouli alcohol and other secondary metabolites such as terpenes other than artemisinin.Type: GrantFiled: June 3, 2011Date of Patent: July 2, 2013Assignee: Firmenich Aromatics (China) Co., Ltd.Inventors: Ke Xuan Tang, Yue Yue Wang, Yue Li Tang, Dong-Fang Chen
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Patent number: 8476492Abstract: The invention relates to a method of utilizing the pts gene and antisense ads to increase patchouli alcohol content in Artemisia annua L. plants. Using transgenic Artemisia annua L. plants, the method of the invention consistently increases the patchouli alcohol content in those plants, thus laying down a solid foundation for large-scale production of patchouli alcohol and other secondary metabolites such as terpenes other than artemisinin.Type: GrantFiled: June 3, 2011Date of Patent: July 2, 2013Assignee: Firmenich Aromatics (China) Co., Ltd.Inventors: Ke Xuan Tang, Yue Yue Wang, Yue Li Tang, Dong-Fang Chen
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Publication number: 20130150436Abstract: A polyacetylenes and application thereof. The polyacetylenes is isolated from an extract of the sporophores of Antrodia Cinnamomea and has a function of inhibiting the production of nitric oxide. Therefore, the polyacetylenes can be used for preparing a pharmaceutical composition for anti-inflammation. The present invention also teaches the representative metabolites of the sporophores of Antrodia Cinnamomea, which can be used to evaluate the quality thereof.Type: ApplicationFiled: December 12, 2011Publication date: June 13, 2013Applicants: National Chung Hsing University, Taiwan Leader Biotech Corp.Inventor: SHENG-YANG WANG
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Publication number: 20130135610Abstract: The present invention is directed to a device and a method for performing size exclusion chromatography. Embodiments of the present invention feature devices and methods for size exclusion chromatography at normal high performance liquid chromatography or ultra performance liquid chromatography pressures and above using small particles.Type: ApplicationFiled: December 15, 2010Publication date: May 30, 2013Applicant: Waters Technologies CorporationInventors: Edouard S.P. Bouvier, Kevin D. Wyndham, Thomas H. Walter, Lynda Neue
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Publication number: 20130130285Abstract: This invention provides reagents and methods for diagnosing renal disease. Differential levels of inosine metabolite, and proteins: apolipoprotein C-I, apolipoprotein C-II, fibrinogen alpha chain, or fibrinogen A-alpha chain, kininogen, Inter-Alpha Inhibitor H4 (ITIH4), keratin Type I cytoskeletol 10 cystatin A, cystatin B and other polypeptides and fragments thereof provide biomarkers of renal disease and are described herein.Type: ApplicationFiled: June 3, 2011Publication date: May 23, 2013Applicant: IDEXX LABORATORIES, INC.Inventors: Michael Randolph Atkinson, Mahalakshmi Yerramilli, Murthy V.S.N. Yerramilli
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Publication number: 20130124105Abstract: Disclosed herein is a method of predicting sediment content of a hydroprocessed hydrocarbon product. The method involves: (a) precipitating an amount of asphaltenes from a liquid sample of a first hydrocarbon-containing feedstock having solvated asphaltenes therein with one or more first solvents; (b) determining one or more solubility characteristics of the precipitated asphaltenes; (c) analyzing the one or more solubility characteristics; (d) determining asphaltene content of the liquid sample from the results of analyzing the one or more solubility characteristics; (e) determining one or more asphaltene stability parameters of the liquid sample from the results of analyzing the one or more solubility characteristics; and (f) correlating the asphaltene content and one of the asphaltene stability parameters of the liquid sample with at least two operation conditions associated with a refinery to predict sediment content.Type: ApplicationFiled: November 11, 2011Publication date: May 16, 2013Applicant: Chevron U.S.A. INCInventors: Estrella Rogel, Cesar Ovalles, Pak Leung, Nan Chen
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Patent number: 8435795Abstract: Methods of evaluating heparin preparations, e.g., for suitability for use as a drug or for use in making a drug, by determining the absence, presence or amount of a structural signature that is indicative of the methods used to make the heparin preparation.Type: GrantFiled: January 18, 2011Date of Patent: May 7, 2013Assignee: Momenta Pharmaceuticals, Inc.Inventors: Daniela Beccati, Ishan Capila, Nur Sibel Gunay, Sucharita Roy
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Publication number: 20130101578Abstract: The invention provides kits and methods for the diagnosis, prognosis, and treatment of reduced kidney function in patients, such as diabetic patients. The methods include a step of detecting one or more nephrin degradation products in a sample from the subject, such as a urine sample.Type: ApplicationFiled: April 12, 2011Publication date: April 25, 2013Applicant: NATIONAL UNIVERSITY OF SINGAPOREInventors: Peng Keat Daniel Ng, Bee Choo Tai, Kee Seng Chia, Harry Holthofer
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Patent number: 8425842Abstract: A suppressor which comprises: an ion-exchange membrane; an eluate channel which is in contact with one side of the ion-exchange membrane, serves as a channel through which an eluate discharged from a separation column flows, and has inside no obstacle to the flow; a regenerant channel which is in contact with the other side of the ion-exchange membrane, serves as a channel through which a regenerant for regenerating ionic functional groups of the ion-exchange membrane flows, has been disposed so that the regenerant channel has no region facing the eluate channel and extends in parallel to the eluate channel in such a nearby position that the ionic functional groups can move through the ion-exchange membrane, and has inside no obstacle to the flow; and an ion-exchange membrane support member which is in contact at least with that region on one side of the ion-exchange membrane which is opposed to the regenerant channel and with that region on the other side of the ion-exchange membrane which is opposed to theType: GrantFiled: January 7, 2008Date of Patent: April 23, 2013Assignee: Shimadzu CorporationInventor: Shigeyoshi Horiike
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Publication number: 20130079236Abstract: Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.Type: ApplicationFiled: September 26, 2011Publication date: March 28, 2013Applicant: Theranos, Inc., a Delaware CorporationInventor: Elizabeth Holmes