The Polynucleotide Contains A Tissue, Organ, Or Cell Specific Promoter Patents (Class 800/287)
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Patent number: 8674176Abstract: The present invention relates to enzymes involved in lipid metabolism. In particular, the present invention provides coding sequences for Arabidopsis Desaturases (ADS), the encoded ADS polypeptides, and methods for using the sequences and encoded polypeptides, where such methods include decreasing and increasing saturated fatty acid content in plant seed oils.Type: GrantFiled: January 13, 2010Date of Patent: March 18, 2014Inventors: Ingo H. Heilmann, John Shanklin
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Publication number: 20140075597Abstract: Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and active fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or active fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part.Type: ApplicationFiled: September 6, 2013Publication date: March 13, 2014Inventors: Marc C Albertsen, Howard P. Hershey, Michael W. Lassner, Yongzhong Wu
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Patent number: 8669108Abstract: The invention provides the isolated promoter polynucleotide sequence of SEQ ID NO:1, and fragments and variants thereof. The invention also provides constructs, plant cells and plants genetically modified to contain the promoter polynucleotide. The invention also provides methods for producing plants with altered gene expression and traits via genetic transformation of plants with the promoter polynucleotides.Type: GrantFiled: March 30, 2009Date of Patent: March 11, 2014Assignee: Vialactia Biosciences (NZ) LimitedInventors: Sathish Puthigae, Jonathan Robert Phillips, Nimali Piyushika Withana, Claudia Jeannette Smith-Espinoza, Catherine Jane Bryant, Kerry Robert Templeton, Shivendra Bajaj
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Patent number: 8658779Abstract: A transcriptional regulatory region of perilla anthocyanin 3-acyltransferase is useful for altering the flower color of plants.Type: GrantFiled: March 9, 2010Date of Patent: February 25, 2014Assignee: Suntory Holdings LimitedInventors: Yoshikazu Tanaka, Masako Mizutani
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Patent number: 8653331Abstract: A process for enhanced photobiological H2 production using transgenic alga. The process includes inducing exogenous genes in a transgenic alga by manipulating selected environmental factors. In one embodiment inducing production of an exogenous gene uncouples H2 production from existing mechanisms that would downregulate H2 production in the absence of the exogenous gene. In other embodiments inducing an exogenous gene triggers a cascade of metabolic changes that increase H2 production. In some embodiments the transgenic alga are rendered non-regenerative by inducing exogenous transgenes for proton channel polypeptides that are targeted to specific algal membranes.Type: GrantFiled: December 29, 2009Date of Patent: February 18, 2014Inventor: James Weifu Lee
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Patent number: 8653325Abstract: The present invention provides methods and compositions for increasing the seed size and/or seed number in plants. In particular, the methods and compositions provide for the over expression of a plant growth and/or development related or associated gene during embryo development. Transgenic plants transformed with genetic constructs having the plant growth and/or development associated gene under the control of an early phase-specific embryo promoter provides mature plants in the field that produce larger and/or more seeds. Methods for selection growth and development associated genes that provide transgenic plants with a higher yield phenotype are also provided.Type: GrantFiled: December 15, 2006Date of Patent: February 18, 2014Assignee: Targeted Growth, Inc.Inventors: Jay DeRocher, Thu Nguyen
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Patent number: 8648229Abstract: The present invention relates generally to transcriptional control sequences for effecting expression of a nucleotide sequence of interest in a plant. More particularly, the present invention relates to transcriptional control sequences that direct specific or preferential expression of an operably connected nucleotide sequence of interest in a plant seed or one or more particular cell or tissue types therein.Type: GrantFiled: September 12, 2008Date of Patent: February 11, 2014Assignee: Australian Centre for Plant Functional Genomics PTY LtdInventors: Sergiy Lopato, Nataliya Kovalchuk, Ainur Ismagul, Jessica Anne Smith
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Patent number: 8648230Abstract: Regulatory regions are shown which regulate expression of an operably linked heterologous nucleic acid molecule in plants. Promoters are described which express at lower levels in pollen cells that in other plant cells. Methods of using such promoter to regulate expression of an operably linked nucleic acid molecule are described. A polyadenylation nucleotide sequence from soybean is further shown.Type: GrantFiled: March 18, 2011Date of Patent: February 11, 2014Assignee: MS Technologies, LLCInventors: Bruce Held, Vaithilingam Sekar, Janell C. Eby, Carol J. Lewnau, Penny Perkins
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Patent number: 8648228Abstract: Nucleotide sequences of a Msca1 gene, critical to male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in impacting male fertility in plants.Type: GrantFiled: February 7, 2011Date of Patent: February 11, 2014Assignees: Pioneer Hi Bred International Inc, E I du Pont de Nemours and CompanyInventors: Marc C. Albertsen, Tim Fox, Mary Trimnell, Yongzhong Wu, Keith Lowe, Bailin Li, Marianna Faller
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Publication number: 20140041073Abstract: Polynucleotides and polypeptides incorporated into expression vectors are introduced into plants and were ectopically expressed. These polypeptides may confer at least one regulatory activity and increased photosynthetic resource use efficiency, increased yield, greater vigor, greater biomass as compared to a control plant.Type: ApplicationFiled: March 13, 2013Publication date: February 6, 2014Applicant: MENDEL BIOTECHNOLOGY, INC.Inventors: Colleen M. Marion, Graham J. Hymus, T. Lynne Reuber, Oliver J. Ratcliffe
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Patent number: 8642839Abstract: Compositions and methods for transiently expressing proteins in a plant are provided. The compositions comprise plants, seeds, plant tissues, and plant parts expressing a protein, wherein the protein is expressed transiently and the transient expression of the protein can be used as a predictive model of how said protein will be expressed in stable transgenic plants in regards to qualitative and quantitative data. The predictive model may be used but is not limited to: promoter evaluation, evaluation of expression cassette construction for best performance (e.g. addition of enhancers or gene silencing suppressors), evaluation of best ways to express heterologous genes (e.g. point mutations, targeting), fast evaluation of endogenous gene knockout, evaluation of protein expression levels, cellular targeting, tissue targeting, transcriptional enhancers, translational enhancer protein toxicity and metabolic profiling. Further provided are methods of use.Type: GrantFiled: June 11, 2010Date of Patent: February 4, 2014Assignee: Syngenta Participations AGInventor: Kasimalai Azhakanandam
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Patent number: 8642749Abstract: A Zea mays regulatory region is shown, which provides improved seed preferred, and particularly embryo preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the embryo tissue of a plant. The sequence is particularly useful in expression of heterologous proteins to the embryo of monocotyledonous plants, particularly cereals, and maize.Type: GrantFiled: October 9, 2010Date of Patent: February 4, 2014Assignee: Applied Biotechnology InstituteInventors: Stephen J Streatfield, Robert Love, Jeff Bray
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Publication number: 20140033366Abstract: The present invention relates generally to the field of molecular biology and concerns a method for improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a GRP (Growth-Related Protein). The present invention also concerns plants having modulated expression of a nucleic acid encoding a GRP, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. The GRP may be one of the following: Seed Yield Regulator (SYR), FG-GAP, CYP90B, CDC27, AT-hook transcription factors, DOF transcription factors and Cyclin Dependent Kinase Inhibitors (CKIs).Type: ApplicationFiled: June 12, 2013Publication date: January 30, 2014Applicant: CropDesign N.V.Inventors: Valerie Frankard, Christophe Reuzeau, Ana Isabel Sanz Molinero, Christian Dammann
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Patent number: 8633353Abstract: The present invention provides nucleic acid constructs, including plasmids, expression vectors or expression cassettes comprising polynucleotides encoding CCAAT-binding transcription factor polypeptides that have the ability to increase a plant's tolerance to abiotic stress. Polynucleotides encoding functional CCAAT-binding transcription factors were incorporated into expression vectors, introduced into plants, and ectopically expressed. The encoded polypeptides of the invention significantly increased the cold and water deficit tolerance of the transgenic plants, as compared to tolerance to these stresses of control plants.Type: GrantFiled: October 30, 2007Date of Patent: January 21, 2014Assignee: Mendel Biotechnology, Inc.Inventors: Oliver J. Ratcliffe, Jacqueline Heard, Roderick W. Kumimoto, Peter P. Repetti, T. Lynne Reuber, Robert Creelman, Frederick D. Hempel, Neal L. Gutterson, Roger Canales
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Publication number: 20140020134Abstract: The present invention relates to a fruit-specific promoter, which is suitable for the expression in a broader range of developmental stages of a fruit. Provided is a fruit-specific promoter DNA, which consists of a nucleotide sequence having 85% or more identity with the nucleotide sequence as shown in SEQ ID NO: 1 or 2 and has promoter activity in mature-green fruits.Type: ApplicationFiled: March 30, 2011Publication date: January 16, 2014Applicants: INPLANTA INNOVATIONS INC., UNIVERSITY OF TSUKUBA, KAZUSA DNA RESEARCH INSTITUTEInventors: Nobuyo Itou, Hirofumi Kuroda, Ken-ichi Takane, Kou Aoki, Daisuke Shibata, Hiroshi Ezura, Kyoko Tanase
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Patent number: 8629322Abstract: A genome shuffling method for autogamous plants, including producing individuals having the following three traits in a tight coupling linkage by a gene engineering technique selected from a transgenic technique and a gene targeting technique: 1) dominant male sterility, 2) chemical tolerance and 3) lethality inducible by activating an inducible promoter, selecting, from progeny of the individuals, male-sterile individuals by means of the chemical tolerance described in 2) and male-fertile individuals by means of the lethality described in 3), arranging the male-sterile individuals and the male-fertile individuals close together in flowering periods thereof, so that the male-sterile individuals are crossed with the male-fertile individuals, harvesting seeds from the male-sterile individuals, and repeating outcrossing using the seeds from generation to generation.Type: GrantFiled: October 29, 2010Date of Patent: January 14, 2014Inventor: Junichi Tanaka
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Patent number: 8624084Abstract: Provided herein are compositions and methods for producing transgenic plants. In specific embodiments, transgenic plants comprise a construct comprising a polynucleotide encoding microRNA167 (miR167), or precursor thereof, operably linked to a plant pericycle-specific promote, wherein the miR167 is ectopically overexpressed in the transgenic plants, and wherein the promoter is optionally a constitutive or inducible promoter. In some embodiments, the transgenic plant has an improved agronomic or nutritional characteristic when cultivated in nitrogen-rich conditions as compared to a wild type plant cultivated in the same conditions. Also provided herein are commercial products (e.g., pulp, paper, paper products, or lumber) derived from the transgenic plants (e.g., transgenic trees) produced using the methods provided herein.Type: GrantFiled: March 17, 2008Date of Patent: January 7, 2014Inventors: Gloria Coruzzi, Kenneth D. Birnbaum, Miriam Gifford, Rodrigo A. Gutierrez
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Patent number: 8626337Abstract: A method and computer program product for distinguishing and sort seeds containing a genetic element of interest from a bulk sample. In various embodiments, the present invention comprises associating a marker with at least some of the seeds containing a genetic element of interest of the bulk sample, exciting the seeds using an electromagnetic energy emitting device, evaluating at least some of the seeds of the bulk sample for the presence or absence of the marker using an evaluating device configured to excite a majority of the surface area of the seeds, and sorting the seeds containing a genetic element of interest based on the presence or absence of the marker.Type: GrantFiled: April 23, 2008Date of Patent: January 7, 2014Assignee: Pioneer Hi Bred International IncInventors: Steven J. Corak, James L. Hunter
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Patent number: 8624086Abstract: The present invention provides a method for disrupting pollen development in a plant, the method comprising inhibiting the expression of an endogenous nucleic acid molecule which is, under normal conditions, detectably expressed in anther tissue of a plant during pollen formation, and which codes for a protein belonging to the MYB class of DNA binding transcription factors. Particularly, the nucleic acid molecule whose expression is blocked encodes MYB 32 or MYB 103. The invention also provides nucleic acid molecules for use in the method, use of the method in producing male sterile plants and transgenic plants produced in accordance with the method.Type: GrantFiled: March 26, 2012Date of Patent: January 7, 2014Assignee: La Trobe UniversityInventors: Roger Parish, Song Li, Sylvana Iacuone, Roger Kalla, Trudi Higginson
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Patent number: 8614367Abstract: Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences are useful in mediating male fertility in plants. In one such method, the homozygous recessive condition of male sterility causing alleles is maintained after crossing with a second plant, where the second plant contains a restoring transgene construct having a nucleotide sequence which reverses the homozygous condition. The restoring sequence is linked with a hemizygous sequence encoding a product inhibiting formation or function of male gametes. The maintainer plant produces only viable male gametes which do not contain the restoring transgene construct. Increase of the maintainer plant is also provided by self-fertilization, and selection for seed or plants which contain the construct.Type: GrantFiled: December 13, 2010Date of Patent: December 24, 2013Assignee: Pioneer Hi Bred International IncInventors: Yongzhong Wu, Howard Hershey
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Patent number: 8604281Abstract: A process of producing transgenic multi-cellular plants or parts thereof expressing a trait of interest that has a controlled distribution of said trait to progeny, comprising (i) producing a first plant or a cell thereof having in a first locus of a nuclear chromosome a first heterologous nucleotide sequence comprising a first fragment of a nucleotide sequence encoding said trait of interest, (ii) producing a second plant or a cell thereof having in a second locus of a nuclear chromosome homologous to said nuclear chromosome of step (i), a second heterologous nucleotide sequence comprising a second fragment of the nucleotide sequence encoding said trait of interest, and (iii) hybridising said first and said second plants or cells thereof to generate progeny exhibiting said functional trait of interest. Also disclosed is a process of producing hybrid seeds for agriculture.Type: GrantFiled: May 14, 2012Date of Patent: December 10, 2013Assignee: Bayer CropScience NVInventors: Stefan Werner, Anatoly Giritch, Serik Eliby, Sylvestre Marillonnet, Victor Klimyuk, Yuri Gleba
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Publication number: 20130326733Abstract: The present invention relates generally to the field of molecular biology and concerns a method for improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a GRP (Growth-Related Protein), specifically a nucleic acid encoding a HAL3 polypeptide, a nucleic acid encoding a MADS15 polypeptide, a nucleic acid encoding a PLT transcription factor polypeptide, a nucleic acid encoding a Squamosa promoter binding protein-like 15 (SPL15) transcription factor polypeptide or a nucleic acid encoding a basic/helix-loop-helix (bHLH) transcription factor. The present invention also concerns plants having modulated expression of a nucleic acid encoding a GRP, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention.Type: ApplicationFiled: June 5, 2013Publication date: December 5, 2013Applicant: BASF Plant Science GmbHInventors: Christophe Reuzeau, Valerie Frankard, Ana Isabel Sanz Molinero
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Patent number: 8598412Abstract: The present invention relates to the field of plant genetic engineering. More specifically, the present invention relates to seed specific gene expression. The present invention provides promoters capable of transcribing heterologous nucleic acid sequences in seeds, and methods of modifying, producing, and using the same.Type: GrantFiled: October 1, 2010Date of Patent: December 3, 2013Assignee: Monsanto Technology LLCInventors: Qi Wang, Tanya Fagaly, Ronald Bassuner, Jihong Liang, Tim N. Oulmassov, John Dabrowski
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Patent number: 8586825Abstract: The present invention relates to a nitrogen-regulated GATA transcription factor gene required for sugar sensing and the modulation of the expression of this gene to modulate a characteristic in a plant. The GATA transcription factor of the present invention is involved in regulating sugar sensing in plants and its expression is influenced by nitrogen status. Increased expression of this or substantially similar genes can produce plants with improved nitrogen utilization and increased yield.Type: GrantFiled: June 29, 2009Date of Patent: November 19, 2013Assignee: The University of GuelphInventors: Steven Rothstein, Yong-Mei Bi
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Patent number: 8586824Abstract: A group of genes including GhCIR1 from cotton (Gossypium hirsutum), and AtCIR1 and AtCIR2 from Arabidopsis thaliana promote shoot regeneration in plants even in the absence of cytokinin. In the presence of cytokinin, the genes significantly improve transformation efficiency. The genes can be used as an enhancer as well as a selectable marker of transformation in plants. The proteins encoded by the novel genes have a homeodomain (HD) at the N-terminus and a highly divergent domain at the C-terminus. The proteins share a common structural motif.Type: GrantFiled: February 13, 2006Date of Patent: November 19, 2013Assignee: Temasek Life Sciences LaboratoryInventors: Lianghui Ji, Lin Cai, Nam-Hai Chua
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Publication number: 20130305413Abstract: A Zea mays regulatory region is shown, which provides improved seed preferred, and particularly pericarp preferred expression in plants. Methods of use are also shown in preferentially expressing a heterologous protein to the pericarp tissue of a plant. The sequence is particularly useful in expression of heterologous proteins to the pericarp of monocotyledonous plants, particularly cereals, and maize.Type: ApplicationFiled: March 7, 2013Publication date: November 14, 2013Applicant: APPLIED BIOTECHNOLOGY INSTITUTEInventors: John Howard, Erin Engelkrout
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Patent number: 8581040Abstract: The present invention describes an alternative approach to increase GABA production in prokaryotes or eukaryotes, namely by the insertion of the putrescine catabolic pathway in organisms where the pathway does not exist or has not clearly been identified. The invention describes methods for the use of polynucleotides that encode functional putrescine aminotransferase (PAT) and gamma-aminobutyricaldehyde dehydrogenase (GABAlde DeHase) polypeptides in plants to increase GABA production. The preferred embodiment of the invention is in plants but other organisms may be used. Changes in GABA availability will improve growth and increase tolerance to biotic and abiotic stress.Type: GrantFiled: April 27, 2011Date of Patent: November 12, 2013Assignee: Plant Sensory Systems, LLCInventors: Frank J. Turano, Kathleen A. Turano
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Patent number: 8581037Abstract: The present invention relates to methods for promoting somatic embryogenesis from a tissue or organ of a plant, by overexpressing a Wuschel gene in said tissue or organ. In one embodiment, such overexpression can be used as a silent selectable marker for transgenic plants. In another embodiment, such expression can be used to confer apomixis to a plant. In another embodiment, such overexpression can be used to create haploid plants, which can be used to produce dihaploid plants.Type: GrantFiled: April 4, 2013Date of Patent: November 12, 2013Assignee: The Rockefeller UniversityInventors: Jianru Zuo, Qi-Wen Niu, Giovanna Frugis, Nam-Hai Chua
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Patent number: 8581044Abstract: The present invention relates to the field of plant genetic engineering. More specifically, the present invention relates to seed specific gene expression during a defined period of embryogenesis. The present invention provides promoters capable of transcribing heterologous nucleic acid sequences in seeds, and methods of modifying, producing, and using the same.Type: GrantFiled: April 4, 2012Date of Patent: November 12, 2013Assignee: Monsanto Technology LLCInventors: Qi Wang, Lisa M. Weaver, Tim N. Oulmassov, Jeffrey Ahrens, Patrice Dubois, Jeffery Q. Shen
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Patent number: 8581041Abstract: The present invention describes an alternative approach to increase GABA production in prokaryotes or eukaryotes, namely by the insertion of the putrescine catabolic pathway in organisms where the pathway does not exist or has not clearly been identified. The invention describes methods for the use of polynucleotides that encode functional putrescine aminotransferase (PAT) and gamma-aminobutyricaldehyde dehydrogenase (GABAlde DeHase) polypeptides in plants to increase GABA production. The preferred embodiment of the invention is in plants but other organisms may be used. Changes in GABA availability will improve growth and increase tolerance to biotic and abiotic stress.Type: GrantFiled: October 17, 2011Date of Patent: November 12, 2013Assignee: Plant Sensory Systems, LLCInventors: Frank J. Turano, Kathleen A. Turano
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Patent number: 8575423Abstract: A method of modifying morphology in a plant by introducing into a plant at least one chimaeric gene having a promoter sequence operably associated with a nucleic acid sequence, the promoter sequence being operable to direct expression in specific cells of the plant and the nucleic acid sequence encoding at least one gene product capable of altering the metabolism of or causing death of the specific cells and/or nearby cells. In particular, the promoter sequence is operable to direct expression in lateral bud or lateral shoot and the nucleic acid encoding at least one gene product capable of disrupting the metabolism of or causing the death of the lateral bud or lateral shoot or nearby cells. Preferably the promoter sequence has the sequence shown as SEQ ID No. 1 or SEQ ID No. 7 or SEQ ID No. 4, or a part thereof capable of regulating expression of a gene, or a sequence having at least 60%, preferably at least 75%, homology to SEQ ID No. 1 or SEQ ID No. 7 and being capable of regulating expression of a gene.Type: GrantFiled: June 14, 2011Date of Patent: November 5, 2013Assignee: Advanced Technologies (Cambridge) LimitedInventors: Christopher John Robert Thomas, Martin Richard Ward
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Patent number: 8569575Abstract: The present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a Yield Enhancing Protein (YEP). The YEP is selected from a Vacuolar Processing Enzyme (VPE) or a CCA1-like polypeptide or a SAP-like polypeptide or a Seed Yield Promoting Factor 1 (SYPF1) polypeptide or a Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) activase (RCA) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding such a YEP, which plants have enhanced yield-related traits relative to control plants. The invention also provides hitherto unknown YEP-encoding nucleic acids, and constructs comprising the same, useful in performing the methods of the invention.Type: GrantFiled: December 21, 2007Date of Patent: October 29, 2013Assignee: BASF Plant Science GmbHInventor: Ana Isabel Sanz Molinero
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Patent number: 8569582Abstract: The invention provides methods for transforming grass plants with Agrobacterium. The invention allows creation of transgenic grass plants without the need for callus as a target tissue for transformation, thus providing a rapid method for the production of transgenic grass plants. Transgenic grass plants produced by this method are also provided.Type: GrantFiled: June 22, 2010Date of Patent: October 29, 2013Assignee: The Samuel Roberts Noble FoundationInventors: Zeng-Yu Wang, Rangaraj Nandakumar
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Patent number: 8563806Abstract: The invention provides isolated promoter polynucleotides that are root-specific and/or induced by plant parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plant roots and are particularly useful for controlling transcription of nucleic acids encoding agents that disrupt formation or maintenance of parasitic nematode feeding sites in plants.Type: GrantFiled: February 4, 2008Date of Patent: October 22, 2013Assignee: BASF Plant Science GmbHInventors: Aaron Wiig, Robert Ascenzi, Xiang Huang, Sumita Chaudhuri, Rui-Guang Zhen, Yu Han
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Publication number: 20130276170Abstract: The present invention relates to materials and methods for the expression of a gene of interest selectively in cotton fibers. In particular, the invention provides an expression cassette for regulating fiber-selective expression in plants.Type: ApplicationFiled: December 21, 2011Publication date: October 17, 2013Applicant: Commonwealth Scientific and Industrial Research OrganizationInventors: Frank Meulewaeter, Danny Llewellyn
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Patent number: 8558058Abstract: Expression of recombinant polysaccharide degrading enzymes in plants is described. In one embodiment, expression of the enzyme is preferentially directed to the seed of the plant. Expression may also be preferentially targeted to specific locations within the plant cell. Expression of cellulases in corn is shown. The result is the capacity to produce polysaccharide degrading enzymes in plants at commercially acceptable levels in a reliable manner. Methods of using same in production of ethanol is also described, including use of the plant-produced enzymes in the ethanol production process.Type: GrantFiled: September 2, 2005Date of Patent: October 15, 2013Assignee: Applied Biotechnology InstituteInventors: Elizabeth Hood, John Howard
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Patent number: 8552256Abstract: The present inventors identified a novel rice glutelin gene, GluD-1, which is expressed specifically in seeds. The promoter of the GluD-1 gene was confirmed to induce seed-specific gene expression, and to induce expression of downstream genes specifically in the endosperm during the early stage of seed maturation process. More specifically, the GluD-1 promoter can induce strong expression of an exogenous gene in sites including the endosperm.Type: GrantFiled: April 10, 2009Date of Patent: October 8, 2013Assignee: National Institute of Agrobiological SciencesInventors: Fumio Takaiwa, Taiji Kawakatsu
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Patent number: 8546647Abstract: The invention provides plant gene promoters and regulatory elements that are root-specific and/or induced by parasitic nematodes. The promoters of the invention are useful for controlling expression of nucleic acids of interest in plants' roots. The invention also provides expression cassettes including the plant gene promoters and regulatory elements of the invention, transgenic plants containing such expression cassettes, and methods of producing such transgenic plants.Type: GrantFiled: December 12, 2007Date of Patent: October 1, 2013Assignee: BASF Plant Science GmbHInventors: Aaron Wiig, Robert Ascenzi, Xiang Huang, Sumita Chaudhuri, Rui-Guang Zhen, Yu Han
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Patent number: 8541665Abstract: The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.Type: GrantFiled: November 1, 2010Date of Patent: September 24, 2013Assignee: Mendel Biotechnology, Inc.Inventors: Cai-Zhong Jiang, Jacqueline E. Heard, Oliver Ratcliffe, Robert A. Creelman, Luc Adam, T. Lynne Reuber, Jose Luis Riechmann, Volker Haake, Arnold N. Dubell, James S. Keddie, Bradley K. Sherman
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Patent number: 8536404Abstract: The invention provides expression vectors comprising root-specific promoters in operative association with polynucleotides that are down-regulated in syncytia of nematode-infected plants, for use in methods of producing transgenic plants with increased resistance to nematode infestation. The invention also provides nematode-resistant transgenic plants and seeds comprising such expression vectors.Type: GrantFiled: November 30, 2009Date of Patent: September 17, 2013Assignee: BASF Plant Science GmbHInventor: Bonnie McCaig
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Patent number: 8536406Abstract: Polynucleotide constructs contain fiber-specific elements which are used to target expression of polynucleotides and polypeptides to the vascular fibers of a plant. The constructs can be contained within a vector. Transgenic plants transformed with the fiber-specific elements can be made which have expression of a polynucleotide or polypeptide directed to the plant fibers.Type: GrantFiled: April 28, 2009Date of Patent: September 17, 2013Assignee: Michigan Technological UniversityInventors: Chung-Jui Tsai, Edward Odhiambo Anino
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Publication number: 20130239254Abstract: The invention provides the disclosure of a novel plant growth pathway involving several receptor like kinases. The pathway was shown to work largely independent of brassinosteroids and involves several members of the CrRLK1L family of receptor kinases. According to the invention, HERK1, HERK2, FER, THE1 and the brassinosteroid BES1 may be modulated to influence plant growth and elongation. The invention includes methods, and transformed plants, cells tissues and seeds with increased cellular elongation and other related yield traits.Type: ApplicationFiled: February 21, 2013Publication date: September 12, 2013Applicant: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.Inventors: Hongqing Guo, Yanhai Yin
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Patent number: 8524979Abstract: This invention provides a genetic construct comprising: (a) a DNA cut-and-paste transposon genetic unit which comprises a transposase gene flanked on either side by inverted repeat sequences and operably under the control of a first promoter; and (b) a transgene unit which comprises an expressable transgene, placed operably under the control of a second promoter; wherein the transposon genetic unit and the transgene unit are linked with one of the inverted repeat sequence located in an intron of the transgene. This invention also provides methods of transiently expressing a transgene in a stably transformed eukaryote. This invention further provides methods for obtaining marker-free transgenic plants.Type: GrantFiled: April 14, 2008Date of Patent: September 3, 2013Assignee: National Taiwan UniversityInventor: Yuh-Chyang Charng
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Publication number: 20130227724Abstract: A method of engineering a plant having reduced acetylation in a cell wall is disclosed. The method comprising expressing in the plant cell wall at least one isolated heterologous polynucleotide encoding an acetylxylan esterase (AXE) enzyme under the transcriptional control of a developmentally regulated promoter specifically active in the plant cell wall upon secondary cell wall deposit, thereby engineering the plant having reduced acetylation in the cell wall.Type: ApplicationFiled: November 3, 2011Publication date: August 29, 2013Applicant: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.Inventors: Miron Abramson, Ziv Shani, Oded Shoseyov
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Publication number: 20130227745Abstract: The invention concerns a method for improving growth characteristics of plants by increasing activity in a plant of an RNA-binding protein which is: (i) a polypeptide having RNA-binding activity and comprising 2 or 3 RNA recognition motifs (RRMs) and a motif having at least 75% identity to motif I: PYEAAVVALPVVVKERLVRILRLGIATRYD (SEQ ID NO: 12) and/or a motif having at least 50% identity to motif II: RFDPFTGEPYKFDP (SEQ ID NO: 13); or (ii) an RBP1 polypeptide having (a) RNA-binding activity; (b) two RRM domains, (c) the following two motifs: (i) KIFVGGL (SEQ ID NO: 41); and (ii) RPRGFGF (SEQ ID NO: 42), allowing for up to three amino acid substitutions and any conservative change in the motifs; and (d) having at least 20% identity to SEQ ID NO: 15. Also provided is transgenic plants introduced with an RNA-binding protein-encoding nucleic acid having improved growth characteristics and constructs useful in the methods.Type: ApplicationFiled: May 3, 2013Publication date: August 29, 2013Applicant: CropDesign N.V.Inventor: CropDesign N.V.
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Publication number: 20130227740Abstract: The present invention discloses transgenic plants having an altered level of NAP protein compared to that of a non-transgenic plant, where the transgenic plants display an altered leaf senescence phenotype relative to a non-transgenic plant, as well as mutant plants comprising an inactivated NAP gene, where mutant plants display a delayed leaf senescence phenotype compared to that of a non-mutant plant. The present invention also discloses methods for delaying leaf senescence in a plant, as well as methods of making a mutant plant having a decreased level of NAP protein compared to that of a non-mutant plant, where the mutant plant displays a delayed leaf senescence phenotype relative to a non-mutant plant. Methods for causing precocious leaf senescence or promoting leaf senescence in a plant are also disclosed. Also disclosed are methods of identifying a candidate plant suitable for breeding that displays a delayed leaf senescence and/or enhanced yield phenotype.Type: ApplicationFiled: February 28, 2013Publication date: August 29, 2013Applicant: CORNELL RESEARCH FOUNDATION, INC.Inventor: Cornell Research Foundation, Inc.
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Patent number: 8519114Abstract: The present invention relates to expression cassettes comprising transcription regulating sequences with constitutive expression profiles in plants obtainable from Arabidopsis thaliana genes At5g17920, At3g03780, At2g01100, At2g34770, At5g61560, At4g00830, At3g10220, At4g38520, At3g11110, At2g47170, Atlg64090, At5g60690, Atlg76350, At1g76580, Atlg31930, At5g18230, Atlg20970, or At4g35620. The expression cassettes according to the present invention may be employed for expression of a protein, or expression of an antisense RNA, sense or double-stranded RNA. Preferably, expression of the nucleic acid sequence confers to the plant an agronomically valuable trait. Vectors comprising such an expression cassette, and transgenic host cell or non-human organism comprising the expression cassette or the vector are also enclosed. Also enclosed is a method for identifying and/or isolating a sequence with constitutive transcription regulating activity.Type: GrantFiled: October 27, 2011Date of Patent: August 27, 2013Assignee: SunGene GmbHInventors: Ulrich Keetman, Ute Linemann, Karin Herbers, Helke Hillebrand
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Publication number: 20130219559Abstract: A novel method to produce hydrophobin monomers or multimers in plant tissues is disclosed. A novel method to produce hydrophobin multimers in E. coli cells is also described. The disclosure provides transgenic plants, transgenic seeds, a production system, and expression cassettes for making the transgenic plant carrying hydrophobin encoding gene sequences.Type: ApplicationFiled: February 21, 2013Publication date: August 22, 2013Inventor: Kimmo Koivu
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Publication number: 20130219560Abstract: The invention provides methods for transgenically controlling physiological post-harvest deterioration (PPD) in plants. Among other aspects, the invention also provides transgenic plants produced by the methods and constructs for creating the plants. According to the present invention, PPD is controlled by expressing one or more transgenes which modulate ROS production or cyanogen levels. Examplary transgenes include alternative oxidase, ROS scavengers, carotenoid biosynthesis genes, and cyanogen metabolism.Type: ApplicationFiled: September 2, 2011Publication date: August 22, 2013Inventor: Richard T. Sayre
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Patent number: 8513491Abstract: Dehydrin- and late embryogenic abundant (LEA) protein-encoding polynucleotides from coffee plants are disclosed. Also disclosed are a promoter sequence from a coffee dehydrin gene, and methods for using these polynucleotides and promoter sequences for gene regulation and manipulation of flavor, aroma, stress tolerance and other features of coffee beans.Type: GrantFiled: July 5, 2006Date of Patent: August 20, 2013Assignees: Nestec S.A., Cornell Research FoundationInventors: Mohamed Ben Amor, James Gèrard McCarthy, Vincent Petiard, Steven D. Tanksley, Chenwei Lin