The Polynucleotide Contains A Tissue, Organ, Or Cell Specific Promoter Patents (Class 800/287)
-
Publication number: 20130007914Abstract: The invention provides several promoters isolated from Oryza sativa, which promoters are capable of driving and/or regulating the expression of an operably linked nucleic acid in a plant. The expression patterns of the promoters according to the invention have been studied in Oryza sativa and some of the promoters displayed specific activity in particular cells, tissues or organs of the plant, while others displayed constitutive expression throughout substantially the whole plant. Some promoters showed weak expression, while others were strongly active.Type: ApplicationFiled: May 15, 2012Publication date: January 3, 2013Inventors: Yves Hatzfeld, Willem Broekaert
-
Patent number: 8344206Abstract: The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.Type: GrantFiled: May 7, 2010Date of Patent: January 1, 2013Assignees: Pioneer Hi Bred International Inc, E.I. du Pont de Nemours and CompanyInventors: Scott Diehn, Albert L. Lu, Carl R. Simmons
-
Patent number: 8338662Abstract: The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.Type: GrantFiled: May 7, 2010Date of Patent: December 25, 2012Assignees: Pioneer Hi Bred International Inc, E.I. du Pont de Nemours and CompanyInventors: Scott Diehn, Albert L. Lu, Carl R. Simmons
-
Patent number: 8334427Abstract: The present invention generally provides a method to generate broad-spectrum resistance to bacterial blight disease in plants. More specifically, the present invention provides a method to generate broad-spectrum resistance to Xanthomonas oryzae pv. oryzae, the causal agent of bacterial blight disease of rice, and enhanced resistance to Xanthomonas oryzae pv. oryzicola, the causal agent of bacterial leaf streak of rice. Xa27, an inducible bacterial blight R gene in rice, was induced by the cognate avrXa27 gene expressed in host. Rice plants carrying the avrXa27 transgene and wild-type Xa27 gene conferred resistance to incompatible and compatible pathogens, and enhanced resistance to X. oryzae pv. oryzicola strain L8. The Xa27-mediated enhanced resistance to X. oryzae pv. oryzicola was also observed in the interaction between IRBB27 and L8 harboring pHM1avrXa27. This was further verified by the fact that the Xa27 gene in IRBB27 was induced by the avrXa27 gene in bacteria.Type: GrantFiled: January 8, 2008Date of Patent: December 18, 2012Assignee: Temasek Life Sciences LaboratoryInventors: Zhongchao Yin, Dongsheng Tian
-
Publication number: 20120317679Abstract: The present invention relates to the field of plant genetic engineering. More specifically, the present invention relates to seed specific gene expression during a defined period of embryogenesis. The present invention provides promoters capable of transcribing heterologous nucleic acid sequences in seeds, and methods of modifying, producing, and using the same.Type: ApplicationFiled: April 4, 2012Publication date: December 13, 2012Inventors: Qi Wang, Lisa M. Weaver, Tim N. Oulmassov, Jeffrey Ahrens, Patrice Dubois, Jeffery Q. Shen
-
Publication number: 20120311742Abstract: The present invention relates to methods for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a cytokinin oxidase in plants or plant parts, preferably in seeds, embryos and/or cotyledons. The methods result in increases in: yield, growth of seedlings, early vigor, and stress tolerance. The invention also relates to isolated plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising such sequences.Type: ApplicationFiled: May 11, 2012Publication date: December 6, 2012Inventors: Thomas Schmulling, Tomàs Werner
-
Patent number: 8324454Abstract: The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of improved plant size, vegetative growth, growth rate, seedling vigor and/or biomass in plants challenged with saline conditions. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having plant size, vegetative growth, growth rate, seedling vigor and/or biomass that are improved in saline conditions with respect to wild-type plants grown under similar conditions.Type: GrantFiled: March 14, 2007Date of Patent: December 4, 2012Assignee: Ceres, Inc.Inventors: Fasong Zhou, Kenneth A. Feldmann, Julissa Sosa
-
Patent number: 8314290Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.Type: GrantFiled: June 26, 2007Date of Patent: November 20, 2012Inventors: Edwards Allen, Sara Elizabeth Heisel, Sergey Ivashuta, Elysia Katherine Krieger, Jennifer Lynn Lutke, Robert Joseph Meister, Yuanji Zhang
-
Patent number: 8304607Abstract: The presently disclosed subject matter includes nucleic acid sequences and expression cassettes that contain regulatory sequences derived from Solanum tuberosum chitinase C genes, for expression of recombinant gene products in plants. The expression cassette drives high transgene expression in the epidermal (L1) cell layer.Type: GrantFiled: December 21, 2009Date of Patent: November 6, 2012Assignee: Syngenta Participations AGInventors: Michael Nuccio, A. Todd Richmond
-
Patent number: 8304602Abstract: The present invention provides methods for regulating the development of apical bud formation in a plant comprising the step of modulating the expression of PtFD1 or a protein having substantial identity to PtFD1, in the plant. Transgenic poplar trees that either overexpress PtFD1 or that down regulate PtFD1 are also provided. Also provided are methods for identifying the regulatory targets of PtFD1.Type: GrantFiled: November 20, 2007Date of Patent: November 6, 2012Assignee: University of MarylandInventor: Gary Dale Coleman
-
Publication number: 20120278914Abstract: The invention provides methods and compositions for the modulation of proanthocynidin (“PA;” condensed tannin) production in plants. The methods of the invention allow creation of plants having novel phenotypes, such as alterations in levels of PA or in the types of tissues in which PAs are produced. Altered expression of PA in plants may be achieved, for instance without significantly affecting anthocyanin content or overall secondary metabolite profiles. Alternatively, expression of PA in plants may be achieved while also affecting anthocyanin content.Type: ApplicationFiled: April 11, 2012Publication date: November 1, 2012Inventors: Jerome A. Verdier, Jian Zhao, Richard A. Dixon, Michael K. Udvardi
-
Patent number: 8299318Abstract: The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides. The present invention further relates to the uses of these nucleic acid molecules and polypeptides. For example, the nucleic acid molecules and polypeptides could be used in making enzymes or used to make plants, plant cells, plant materials or seeds of a plant having such modulated growth or phenotype characteristics that are altered with respect to wild type plants grown under similar conditions.Type: GrantFiled: July 7, 2008Date of Patent: October 30, 2012Assignee: Ceres, Inc.Inventors: Vyacheslav Brover, Timothy J. Swaller, Kenneth A Feldmann, Maxim Troukhan
-
Publication number: 20120272407Abstract: The invention provides isolated nucleic acids and their encoded polypeptides that alter tocol content in seeds. The invention further provides expression cassettes, host cells and transformed plants containing the nucleic acids. The present invention further provides methods for altering tocol content in seeds.Type: ApplicationFiled: July 5, 2012Publication date: October 25, 2012Applicant: E.I. Dupont de Neumours and CompanyInventors: Edgar B. Cahoon, Sean J. Coughlan, Rebecca E. Cahoon, Karlene H. Butler
-
Patent number: 8283519Abstract: The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, variants of naturally-occurring sequences, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties, including improved cold and other osmotic stress tolerance, as compared to wild-type or reference plants. The invention also pertains to expression systems that may be used to regulate these transcription factor polynucleotides, providing constitutive, transient, inducible and tissue-specific regulation.Type: GrantFiled: May 4, 2004Date of Patent: October 9, 2012Assignee: Mendel Biotechnology, Inc.Inventors: Robert A. Creelman, Oliver Ratcliffe, Roderick W. Kumimoto, Neal I. Gutterson, T. Lynne Reuber, Jeffrey M. Libby, Jacqueline E. Heard, Jose Luis Riechmann, Omaira Pineda
-
Patent number: 8269061Abstract: Methods of selecting haploid embryos are disclosed. Methods of producing haploid embryos and non-viable diploid embryos on a plant are provided. Methods for selecting haploid embryos produced from haploid inducer maize lines are provided. Methods for producing improved maize haploid inducer lines are disclosed. Maize haploid inducer lines comprising transgenes causing ablated or abnormal diploid embryos are disclosed.Type: GrantFiled: October 3, 2011Date of Patent: September 18, 2012Assignee: E.I. du Pont de Nmeours and CompanyInventor: Mark E. Williams
-
Patent number: 8263758Abstract: The present invention discloses a promoter sequence of light-harvesting chlorophyll a/b binding protein pGWLS01 isolated from the oil palm leaf. This promoter enables the manipulation of oil palm leaves for the production of high value-added products via genetic engineering tools. The novel features of the promoter itself which regulate high and specific expression of foreign genes in the leaves will avoid the interference of novel products in the commodity oil extracted from mesocarp and kernel tissues. Furthermore, the promoter is also potentially useful in the production of insect-resistant palm.Type: GrantFiled: December 7, 2009Date of Patent: September 11, 2012Assignee: Malaysian Palm Oil BoardInventors: Pek Lan Chan, Siti Nor Akmar Abdullah
-
Patent number: 8263826Abstract: The invention provides promoter polynucleotides that are root-preferred and/or induced by parasitic nematodes. The promoter polynucleotides of the invention are useful for controlling expression of nucleic acids of interest in plant roots.Type: GrantFiled: February 4, 2008Date of Patent: September 11, 2012Assignee: BASF Plant Science GmbHInventors: Aaron Wiig, Xiang Huang, Sumita Chaudhuri
-
Patent number: 8252977Abstract: Polynucleotides encoding polypeptides that comprise the biosynthetic pathway for carotenoids and apocarotenoids in the coffee plant are disclosed. Also disclosed are a promoter sequence from a coffee carotenoid gene, and methods for using these polynucleotides, polypeptides, and promoter sequences for gene regulation and the manipulation of flavor, aroma, and other features of coffee beans, as well as the manipulation of photosynthesis in the coffee plant.Type: GrantFiled: September 1, 2006Date of Patent: August 28, 2012Assignees: Nestec S. A., Cornell UniversityInventors: Steven D. Tanksley, Chenwei Lin, Andrew Simkin, James Gérard McCarthy, Vincent Petiard
-
Patent number: 8247649Abstract: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a root-preferred promoter and terminator isolated from the maize MSY coding region. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.Type: GrantFiled: September 11, 2008Date of Patent: August 21, 2012Assignee: Pioneer Hi Bred International Inc.Inventors: Xiaomu Niu, Bruce J Drummond
-
Publication number: 20120210463Abstract: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter for the gene encoding Sorghum bicolor RCc3. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the promoters of the present invention.Type: ApplicationFiled: February 13, 2012Publication date: August 16, 2012Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Scott Diehn, Brooke Peterson-Burch
-
Publication number: 20120198587Abstract: Gene expression is controlled at the transcriptional level by very diverse group of proteins called transcription factors (TFs). 5671 soybean (Glycine max) genes have been identified and disclosed as putative transcription factors through mining of soybean genome sequences. Distinct classes of the TFs are also disclosed which may be expressed and or function in a manner that is tissue specific, developmental stage specific, biotic and/or abiotic stress specific. Manipulation and/or genetic engineering of specific transcription factors may improve the agronomic performance or nutritional quality of plants. Transgenic plants expressing a select number of these TFs are disclosed. These transgenic plants show some promising traits, such as improving the capability of the plant to grow and reproduce under drought conditions.Type: ApplicationFiled: June 30, 2010Publication date: August 2, 2012Inventors: Henry T. Nguyen, Gary Stacey, Dong Xu, Jianlin Cheng, Trupti Joshi, Marc Libault, Babu Valliyodan
-
Patent number: 8232380Abstract: Regulatory regions suitable for directing the expression of a heterologous polynucleotide under light conditions in which the red/far red ratio is less than 1 are described, as well as nucleic acid constructs that include these regulatory regions. Also disclosed are transgenic plants that contain such constructs and methods of producing such transgenic plants.Type: GrantFiled: August 25, 2008Date of Patent: July 31, 2012Assignee: Ceres, Inc.Inventors: Shing Kwok, Amy Jo Miyamoto, Kenneth Bounds
-
Patent number: 8227664Abstract: The invention concerns a method for increasing plant yield by introducing into a plant a cyclin A nucleic acid, preferably encoding a cyclin A protein, which cyclin A nucleic acid is operably linked to a seed-preferred promoter. By using this method, plant yield may be increased in optimal and sub-optimal growing conditions. The method results in plants having increased yield relative to corresponding wild type plants and relative to transgenic plants constitutively expressing cyclin A.Type: GrantFiled: December 22, 2004Date of Patent: July 24, 2012Assignee: CropDesign N.V.Inventors: Valerie Frankard, Vladmir Mironov
-
Patent number: 8227663Abstract: The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. Also provided by the present invention are methods for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a cytokinin oxidase or expression of another protein that reduces the level of active cytokinins in plants or plant parts. Methods and compositions for increasing seed yield are also provided. The invention also relates to isolated plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising such sequences.Type: GrantFiled: November 7, 2008Date of Patent: July 24, 2012Inventors: Thomas Schmülling, Tomàs Werner
-
Patent number: 8222388Abstract: Regulatory regions suitable for directing expression of a heterologous polynucleotide in plant tissues, e.g., flower and silique tissues, are described, as well as nucleic acid constructs that include these regulatory regions. Also disclosed are transgenic plants that contain such constructs and methods of producing such transgenic plants.Type: GrantFiled: November 16, 2007Date of Patent: July 17, 2012Assignee: Ceres, Inc.Inventor: Tatiana Tatarinova
-
Patent number: 8222387Abstract: The present invention relates generally to transcriptional control sequences for effecting expression of a nucleotide sequence of interest in a plant. The present invention is predicated, in part, on the identification and functional characterization of transcriptional control sequences derived from genes which encode polypeptides comprising the amino acid sequence set forth in SEQ ID NO: 1 and homologs thereof. Among other things, the present invention has identified that transcriptional control sequences derived from the subject genes can effect specific or preferential expression of an operably connected nucleotide sequence in a reproduction-associated plant part such as a seed or anther.Type: GrantFiled: May 31, 2007Date of Patent: July 17, 2012Assignees: Grains Research & Development Corporation, Adelaide Research & Innovation Pty LtdInventors: Sergiy Lopato, Ming Li
-
Patent number: 8217227Abstract: The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.Type: GrantFiled: October 10, 2006Date of Patent: July 10, 2012Assignee: Monsanto Technology LLCInventors: Edwards Allen, Larry A. Gilbertson, Shihshieh Huang, Thomas Malvar, James K. Roberts
-
Patent number: 8207399Abstract: The present invention relates to a method for producing syringyl lignin in gymnosperms. The production of syringyl lignin in gymnosperms is accomplished by genetically transforming a gymnosperm genome, which does not normally contain genes which code for enzymes necessary for production of syringyl lignin, with DNA which codes for enzymes found in angiosperms associated with production of syringyl lignin. The expression of the inserted DNA is mediated using host promoter regions in the gymnosperm.Type: GrantFiled: June 24, 2010Date of Patent: June 26, 2012Assignee: Arborgen Inc.Inventors: Vincent L. Chiang, Daniel T. Carraway, Richard H. Smeltzer
-
Publication number: 20120159670Abstract: The present invention is in the field of plant molecular biology and provides methods for production of high expressing seed-specific and/or seed-preferential promoters and the production of plants with enhanced seed-specific and/or seed-preferential expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to said promoters and/or introduced into plants.Type: ApplicationFiled: August 11, 2010Publication date: June 21, 2012Applicant: BASF PLANT SCIENCE COMPANYInventors: Josef Martin Kuhn, Linda Patricia Loyall, Malte Siebert, Elke Duwenig
-
Publication number: 20120156718Abstract: The present invention relates to optimized ketolase coding sequences, corresponding coding sequences and genetic constructs alone or in combination with beta-cyclase coding sequences, their use for the expression in plants, in particular in plants of the genus Tagetes, to such genetically modified plants, to a process for the preparation of carotenoid products by culturing the genetically modified plants. The present invention further relates to the expression of optimized ketolase coding sequences alone or in combination with beta-cyclase coding sequences under control of an Antirrhinum majus ANTHIRRHINUM FIDDLEHEAD (AFI) promoter, In addition the invention relates to the use of an AFI promoter for heterologous gene expression, preferably for flower-specific expression of genes in plants of the genus Tagetes, to the genetically modified plants of the genus Tagetes, and to a process for producing biosynthetic products by cultivating the genetically modified plants.Type: ApplicationFiled: December 8, 2009Publication date: June 21, 2012Applicant: BASF Plant Science GmbHInventors: Ralf Flachmann, Irina Wenderoth, Christel Renate Schopter, Bettina Tschiersch, Hannia Bridg-Giannakopoulos, Michael Leps, Ute Linemann, George Mather Sauer
-
Publication number: 20120144529Abstract: The present invention relates generally to the field of molecular biology and concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a FSM1-like (Fruit Sant/Myb) polypeptide, or a PIF3-like (PHYTOCHROME INTERACTING FACTOR) polypeptide, or an Uroporphyrinogen III decarboxylase (UROD) polypeptide, or an AS-MTT (Abiotic Stress Membrane Tethered Transcription factor) polypeptide, or an EXO-1 polypeptide, or a YiAP2 (Yield increasing Apetala 2) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding a FSM1-like polypeptide, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention.Type: ApplicationFiled: August 11, 2010Publication date: June 7, 2012Applicant: BASF Plant Science Company GmbHInventors: Ana Isabel Sanz Molinero, Yves Hatzfeld, Valerie Frankard, Christophe Reuzeau
-
Publication number: 20120144528Abstract: The present disclosure relates to compositions and methods for increasing the leaf-to-root ratio of the signal metabolite 2-oxoglutaramate and related proline molecules in plants by modulating levels of ?-amidase to increase nitrogen use efficiency, resulting in enhanced growth, faster growth rates, greater seed and fruit/pod yields, earlier and more productive flowering, increased tolerance to high salt conditions, and increased biomass yields.Type: ApplicationFiled: February 28, 2011Publication date: June 7, 2012Applicants: University of Maine System Board of Trustees, Los Alamos National Security, LLCInventors: Pat J. Unkefer, Penelope S. Anderson, Thomas J. Knight
-
Patent number: 8193410Abstract: A process of producing a transgenic multi-cellular plants or parts thereof expressing a trait of interest, said trait having a controlled distribution of said trait to progeny, wherein said process comprises (i) producing a first plant or a cell thereof having in a first locus of a nuclear chromosome a first heterologous nucleotide sequence comprising a first fragment of a nucleotide sequence encoding said trait of interest, (ii) producing a second plant or a cell thereof having in a second locus of a nuclear chromosome homologous to said nuclear chromosome of step (i), a second heterologous nucleotide sequence comprising a second fragment of the nucleotide sequence encoding said trait of interest, and (iii) hybridising said first and said second plant or cells thereof to generate progeny exhibiting said functional trait of interest due to binding between a protein or polypeptide encoded by said first heterologous nucleotide sequence and a protein or polypeptide encoded by said second heterologous nucleotideType: GrantFiled: November 19, 2009Date of Patent: June 5, 2012Assignee: Icon Genetics GmbHInventors: Stefan Werner, Anatoly Giritch, Serik Eliby, Sylvestre Marillonnet, Victor Klimyuk, Yuri Gleba
-
Patent number: 8193413Abstract: The present invention relates to the field of plant genetic engineering. More specifically, the present invention relates to seed specific gene expression during a defined period of embryogenesis. The present invention provides promoters capable of transcribing heterologous nucleic acid sequences in seeds, and methods of modifying, producing, and using the same.Type: GrantFiled: October 2, 2009Date of Patent: June 5, 2012Assignee: Monsanto Technology LLCInventors: Qi Wang, Lisa M. Weaver, Tim N. Oulmassov, Jeffrey Ahrens, Patrice Dubois, Jeffrey Q. Shen
-
Patent number: 8178750Abstract: Nucleotide sequences mediating male fertility in plants are described, with DNA molecule and amino acid sequences set forth. Promoter sequences and their essential regions are also identified. The nucleotide sequences and promoter sequences are useful in methods mediating male fertility in plants, as described. Vectors, plant cells, and plants comprising the nucleotide sequences and/or promoter regions are also provided.Type: GrantFiled: July 25, 2011Date of Patent: May 15, 2012Assignee: Pioneer Hi Bred International IncInventors: Marc C Albertsen, Gary A Huffma, Tim W Fox, Mary Trimnell
-
Patent number: 8178751Abstract: The invention relates to a method of generating or increasing a pathogen resistance in plants by reducing the expression of at least one stomatin polypeptide or a functional equivalent thereof. The invention relates to novel nucleic acid sequences coding for a Hordeum vulgare stomatin (HvSTM1) polynucleotide and describes homologous sequences (STM1) thereof, and to their use in methods for obtaining a pathogen resistance in plants, and to nucleic acid constructs, expression cassettes and vectors which comprise these sequences and which are suitable for mediating a fungal resistance in plants. The invention furthermore relates to transgenic organisms, in particular plants, which are transformed with these expression cassettes or vectors, and to cultures, parts or transgenic propagation material derived therefrom.Type: GrantFiled: January 4, 2007Date of Patent: May 15, 2012Assignee: BASF Plant Science GmbHInventors: Markus Frank, Patrick Schweizer, Dimitar Douchkov
-
Patent number: 8173864Abstract: The present invention relates generally to transcriptional control sequences. Generally, the present invention relates to transcriptional control sequences that specifically or preferentially direct expression of a nucleotide sequence of interest in a plant egg cell. The present invention is predicated, in part, on the identification of transcriptional control sequences derived from EC1 genes which, in preferred embodiments, direct preferential expression in an egg cell of at least one plant taxon.Type: GrantFiled: February 13, 2007Date of Patent: May 8, 2012Assignees: Adelaide Research & Innovation Pty Ltd, Grains Research & Development CorporationInventors: Stefanie Sprunck, Birgit Bellman, Thomas Dresselhaus
-
Patent number: 8168863Abstract: Compositions and methods for increasing the expression and/or accumulation of cellobiohydrolase enzyme in the vacuoles of plant cells are provided. The method involves targeting the enzyme to the vacuoles through the use of a barley polyamine oxidase (BPAO) vacuole sorting signal peptide. Plants transformed with an expression construct encoding the vacuole sorting signal peptide operably linked to the cellobiohydrolase enzyme direct expression of the polypeptide to the vacuoles of the plant cells. Transgenic plants, seeds, and plant tissues, and plant parts are provided. Downstream uses of transgenic plants or plant material expressing the constructs of the invention include agronomical and industrial uses, for example, human food, animal feed, biofuel, industrial alcohol, fermentation feedstocks, and the like.Type: GrantFiled: January 26, 2009Date of Patent: May 1, 2012Assignee: Syngenta Participations AGInventor: Stacy Miles
-
Patent number: 8163975Abstract: The present invention provides a method for disrupting pollen development in a plant, the method comprising inhibiting the expression of an endogenous nucleic acid molecule which is, under normal conditions, detectably expressed in anther tissue of a plant during pollen formation, and which codes for a protein belonging to the MYB class of DNA binding transcription factors. Particularly, the nucleic acid molecule whose expression is blocked encodes MYB 32 or MYB 103. The invention also provides nucleic acid molecules for use in the method, use of the method in producing male sterile plants and transgenic plants produced in accordance with the method.Type: GrantFiled: July 28, 2006Date of Patent: April 24, 2012Assignees: LA Trobe University, Grains Research and Development Corporation, Agriculture Victoria Services Pty LtdInventors: Roger Parish, Song Li, Sylvana Iacuone, Roger Kalla, Trudi Higginson
-
Patent number: 8163980Abstract: The present invention provides isolated nucleic acids comprising nucleotide sequences encoding isoprenoid modifying enzymes, as well as recombinant vectors comprising the nucleic acids. The present invention further provides genetically modified host cells comprising a subject nucleic acid or recombinant vector. The present invention further provides a transgenic plant comprising a subject nucleic acid. The present invention further provides methods of producing an isoprenoid compound, the method generally involving culturing a subject genetically modified host cell under conditions that permit synthesis of an isoprenoid compound modifying enzyme encoded by a subject nucleic acid.Type: GrantFiled: June 29, 2006Date of Patent: April 24, 2012Assignee: The Regents of the University of CaliforniaInventors: Dae-Kyun Ro, Karyn Newman, Eric M. Paradise, Jay D. Keasling, Mario Ouellet, Rachel Eachus, Kimberly Ho, Timothy Ham
-
Patent number: 8158853Abstract: Polynucleotides encoding polypeptides that comprise the biosynthetic pathway for lignins in the coffee plant are disclosed. Also disclosed are methods for using these polynucleotides and polypeptides for the manipulation of flavor, aroma, and other features of coffee beans, as well as the manipulation resistance to pathogen, herbivore, and insect attack in the coffee plant.Type: GrantFiled: October 16, 2006Date of Patent: April 17, 2012Assignees: Nestec S. A., Cornell UniversityInventors: Maud Lepelley, James Gérard McCarthy, Vincent Petiard, Gerald Cheminade, Steven D. Tanksley, Chenwei Lin
-
Publication number: 20120090051Abstract: Plant nitrogen use efficiency in corn has been improved by transformation with a flavohemoglobin gene. Plants comprising a flavohemoglobin gene have decreased nitric oxide (NO) levels, increased biomass accumulation under a sufficient nitrogen growth condition, and increased chlorophyll content under a limiting nitrogen growth condition. Additionally, these transformed plants evidence higher levels of yield.Type: ApplicationFiled: October 4, 2010Publication date: April 12, 2012Inventors: Amarjit Basra, Mike Edgerton, Garrett J. Lee, Maolong Lu, Linda L. Lutfiyya, Wei Wu, Xiaoyun Wu
-
Patent number: 8148605Abstract: A eukaryotic input circuit: computationally designed receptors, synthetic eukaryotic signal transduction pathways, and a synthetic signal sensitive promoter that allow highly specific transcriptional induction in response to an externally provided ligand is disclosed. The input circuit is able to specifically bind a targeted substance and transmit a signal to the nucleus where transcription of a gene is activated. An output circuit serves as a simple readout system of the substance detected by the input circuit. The readout circuit exemplified here is a degreening circuit which causes plants to turn white. Activation of the degreening circuit can be detected by eye, or remotely with a variety of machines (hand-held, aircraft or satellite based) and is also resettable. When linked the input circuit if operably linked to the output circuit, produces a functional plant detector.Type: GrantFiled: April 25, 2007Date of Patent: April 3, 2012Assignees: Colorado State University Research Foundation, Duke UniversityInventors: June Medford, Mauricio S. Antunes, Kevin J. Morey, Homme W. Hellinga, James Jefferson Smith
-
Patent number: 8148606Abstract: The object of the invention is to provide a plant body in which the program for accumulating storage products has been modified, and applications of the same. In the invention, the plant body is constructed so as to have a B3 DNA-binding domain and an EAR motif, and so as to be capable of repressing the expression of two or more genes which code for two or more proteins having a sugar-inducible promotor function-suppressing activity.Type: GrantFiled: October 10, 2006Date of Patent: April 3, 2012Assignees: National University Corporation, Nagoya UniversityInventors: Kenzo Nakamura, Hironaka Tsukagoshi, Atsushi Morikami
-
Publication number: 20120079620Abstract: A promoter with an organ-specific activity in plants. The promoter is characterized in that it exhibits greater activity in the storage organs of plants than in other organs of said plants and that the promoter activity is modified after the harvest of the storage organs and is greater than prior to said harvest.Type: ApplicationFiled: September 25, 2011Publication date: March 29, 2012Applicants: SUEDZUCKER AG, KWS SAAT AGInventors: Reinhard Hehl, Alexander Rotthues, Dietmar Juergen Stahl
-
Patent number: 8143475Abstract: The promoter of a soybean lipid transfer protein LTP4 and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants are described.Type: GrantFiled: December 11, 2008Date of Patent: March 27, 2012Assignee: E I du Pont de Nemours and CompanyInventor: Zhongsen Li
-
Patent number: 8143481Abstract: The present application provides Brassica INDEHISCENTI (BIND) sequences.Type: GrantFiled: March 19, 2009Date of Patent: March 27, 2012Assignee: The Regents of the University of CaliforniaInventors: Martin F. Yanofsky, Sherry Kempin
-
Publication number: 20120073018Abstract: The present invention relates to the isolation of Jatropha curcas curcin genes and tissue-specific promoters and to the production of curcin-deficient Jatropha plants. More specifically, the present invention relates to the isolation of Jatropha curcas Curcin 1, Curcin 2 and Curcin 2 A. The present invention further relates to of the Curcin 1, Curcin 2 A and Curcin 2 genes and more particularly to tissue specific promoters of the Curcin 1 and Curcin 2A genes. The present invention further relates to production of curcin-deficient transgenic jatropha plants by using RNAi technology to suppress curcin gene expression.Type: ApplicationFiled: June 2, 2010Publication date: March 22, 2012Applicant: TEMASEK LIFE SCIENCES LABORATORY LIMITEDInventors: Zhong Chao Yin, Li Fang Wu, Hui Zhu Mao, Cheng Xiang Qiu
-
Patent number: 8138389Abstract: The present invention provides nucleotide sequences and corresponding amino acid sequences for plant cytokinin oxidase proteins. In addition, vectors, host cells, and transgenic plants comprising such sequences as well as methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism using such sequences are provided by the present invention. Also provided by the present invention are methods for altering various plant phenotypes including delaying onset to flowering, increasing leaf thickness, reducing vessel size, inducing parthenocarpy, increasing branching, increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight using cytokinin oxidase proteins and/or nucleic acid molecules encoding cytokinin oxidase.Type: GrantFiled: July 27, 2010Date of Patent: March 20, 2012Inventors: Thomas Schmulling, Tomas Werner
-
Publication number: 20120066795Abstract: The present invention provides an isolated promoter or an active fragment or derivative thereof capable of conferring selective expression on a gene to which it is operably connected in the endosperm of a developing plant seed and preferably in the basal endosperm transfer layer (BETL) of endosperm. The present invention also provides expression vectors and constructs and transgenic plant cells, plant parts and whole plants comprising the promoter, active fragments and derivatives, and well as methods of modulating one or more plant phenotypes employing the promoter, active fragments and derivatives.Type: ApplicationFiled: May 12, 2010Publication date: March 15, 2012Applicant: BASF Plant Science Company GmbHInventors: German Spangenberg, Ulrik John, Carl Ramage, Huihua Fu