Abstract: Disclosed are a nanoparticle sensor for measuring protease activity, for protease imaging, and a method for preparing the same. More specifically, the present invention relates to a nanoparticle sensor for measuring protease activity in which a fluorophore- and a quencher-conjugated peptide substrate is conjugated to a biocompatible polymer nanoparticle. The peptide substrate is specifically lysed by a protease. The sensor according to the present invention is capable of inhibiting emission of fluorescence with high extinctive activity of the quencher on a fluorescent material. But strong fluorescence is specifically emitted only if the peptide substrate is lysed by a specific protease. Therefore, the sensor is especially useful as a method for screening a novel drug such as a protease overexpression inhibitor, and early diagnosis of incurable diseases and various diseases such as autoimmune diseases including cancer, osteoarthritis, rheumatoid arthritis and dementia.

Abstract: Compositions and methods for labeling biological targets using a conjugate of a luminescent component and a targeting molecule attached to a nanoparticle core structure are described. The labeling conjugates offer high intensity and low background, and are ideal for histology and pathology.

Abstract: A molecular single electron transistor (MSET) detector device (14) is described that comprises at least one organic molecule (87) connecting a drain electrode (84) and a source electrode (82). In use, said at least one organic molecule (87) provides a quantum confinement region. At least one analyte receptor site (90, 92) is provided in the vicinity of said at least one organic molecule (87) that bind molecules of interest (analytes). A fluid analyser (2) is also described that includes the MSET detector, a pre-concentrator (4) and a fluid gating structure (6). The fluid gating structure (6) is arranged to selectively route fluid from the pre-concentrator (4) to either one of the detector (14) and an exhaust port (12). The pre-concentrator (4), fluid gating structure (6) and detector (14) are each formed as substantially planar layers and arranged in a stack or cube.