Patents Represented by Attorney BioTechnology Law Group
  • Patent number: 6787154
    Abstract: The present invention is directed to artificial antigen presenting cells and methods of making artificial antigen presenting cells. Such artificial antigen presenting cells may be used in certain methods of isolating and expanding T cell populations as well as modulating T cell responses. Additionally, the present invention provides novel methods for the identification and isolation of antigen-specific T cells. The methods provide for the construction of liposomes containing MHC:peptide complexes, accessory molecules, co-stimulatory molecules, adhesion molecules, and other molecules irrelevant to T cell binding or modulation that are used in the binding of artificial antigen presenting cells to solid support systems that may be used in the retrieval and identification of antigen-specific T cells.
    Type: Grant
    Filed: January 9, 2001
    Date of Patent: September 7, 2004
    Inventor: Salvatore Albani
  • Patent number: 6763264
    Abstract: A method for in vivo electrotherapy, or electroporation-mediated therapy, using a needle array apparatus is provided. Treatment of tumors with a combination of electroporation using the apparatus of the invention, and a chemotherapeutic agent, caused regression of tumors in vivo.
    Type: Grant
    Filed: August 6, 2002
    Date of Patent: July 13, 2004
    Assignee: Genetronics, Inc.
    Inventor: Gunter A. Hofmann
  • Patent number: 6748265
    Abstract: An electrode assembly for an apparatus for trans-surface molecular includes a non-conductive carrier having a proximal surface, a distal surface, and a plurality of through holes from the proximal surface to the distal surface, a plurality of first electrodes disposed on the proximal surface, a first conductor disposed on at least a first portion of the distal surface and extending through at least a first portion of the plurality of through holes and connected to the first electrodes on the proximal surface, a plurality of second electrodes disposed on the proximal surface, and a second conductor disposed on at least a second portion of the distal surface and extending through at least a second portion of the plurality of through holes and connected to the second electrodes on the proximal surface, wherein the first electrodes and the second electrodes are configured and disposed in closely spaced relation on the proximal surface for engaging the tissue surface and applying an electric field.
    Type: Grant
    Filed: May 2, 2001
    Date of Patent: June 8, 2004
    Assignee: Genetronics, Inc.
    Inventors: G√ľnter A. Hofmann, Dietmar P. Rabussay, Zei Zhang
  • Patent number: 6746441
    Abstract: A method and apparatus for introducing a preselected molecule into a living cell by contacting the cell with the preselected molecule and applying a multiple series of electrical pulses to the cell. The method can be utilized ex vivo. The multiple electrical pulses generate rotating electric fields which introduce transient pores in the living cell without killing the cell. The rotating electric fields are provided in a flow through chamber apparatus having more than two electrodes. A three-step pulse process, e.g. collection, electroporation, electrophoresis, is used to introduce the preselected molecule into the cell. A mechanical means of repositioning cells between successive pulses is also provided. The apparatus can also provide a means to pulse cells at different temperatures and then after pulsing, let the cells recover for a specified residence time at another temperature.
    Type: Grant
    Filed: December 22, 1999
    Date of Patent: June 8, 2004
    Assignee: Genetronics, Inc.
    Inventors: Gunter A. Hofmann, Dietmar Rabussay
  • Patent number: 6716811
    Abstract: Novel compounds are provided that bind to G-CSFR. The novel compounds have a peptide chain approximately 6 to 40 amino acids in length that binds to G-CSFR. The compounds are useful as probes for affinity screening. In addition, the compounds have demonstrated agonist or antagonist activity for the G-CSFR, and are therefore useful in treatment of diseases including patients who suffer from a low white blood cell titer. Pharmaceutical compositions and methods of use are provided as well.
    Type: Grant
    Filed: July 20, 2000
    Date of Patent: April 6, 2004
    Assignee: Affymax, Inc.
    Inventors: Steven E. Cwirla, Palani Balu, David J. Duffin, Sunila Piplani, Barbara McEowen Merrill, Peter J. Schatz
  • Patent number: 6697669
    Abstract: The present invention describes an in vivo method, using pulsed electric field to deliver therapeutic agents into cells of the skin and muscle for local and systemic treatments. In particular, therapeutic agents include naked or formulated nucleic acid, polypeptides and chemotherapeutic agents.
    Type: Grant
    Filed: July 13, 1999
    Date of Patent: February 24, 2004
    Assignee: Genetronics, Inc.
    Inventors: Nagendu B. Dev, Gunter A. Hofmann, Edward Nolan, Dietmar P. Rabussay, Arnt Tonnessen, Georg Widera, Lei Zhang
  • Patent number: 6678556
    Abstract: In vivo methods are provided for using an electric field to delivery therapeutic treatment to a subject while reducing inducement of histopathological change in the target muscle tissue, such as is associated with induction or amplification of an immune response caused by the pulsed electric field. Therapeutic agents can be delivered nto cells of muscle for local and systemic treatments with optimal gene expression and minimal tissue damage. In particular, therapeutic agents include naked or formulated nucleic acid, polypeptides and chemotherapeutic agents.
    Type: Grant
    Filed: June 7, 2002
    Date of Patent: January 13, 2004
    Assignee: Genetronics, Inc.
    Inventors: Edward Nolan, Dietmar P. Rabussay, Georg Widera, Lei Zhang
  • Patent number: 6678558
    Abstract: A method for delivery of an agent to a cell using electroporation is disclosed. The method includes positioning a first electrode and a second electrode such that an electrical signal passed between the first electrode and the second electrode passes through the cell. The method also includes passing an electrical signal between the first electrode and the second electrode, the electrical signal having a frequency greater than about 10 kHz. In one embodiment of the method, the electrical signal has a bipolar waveform. In another embodiment of the method, the electrodes are positioned at a treatment site, e.g., a tumor, for in vivo delivery of an agent.
    Type: Grant
    Filed: March 23, 2000
    Date of Patent: January 13, 2004
    Assignee: Genetronics, Inc.
    Inventors: Steve Dimmer, Gunter Hofmann, Daniel Holt, Gurvinder Nanda, Edward M. Nolan