Abstract: The present invention relates to a novel laccase enzyme obtainable from the strains of genus Thielavia. The invention relates also to the nucleic acid sequence encoding the enzyme, a recombinant host into which the nucleic acid sequence has been introduced and a method for the production of the enzyme in a recombinant host. The enzyme of the invention is suitable for several applications, in particular for increasing the lightness of denim.
Type:
Grant
Filed:
September 21, 2005
Date of Patent:
April 19, 2011
Assignee:
AB Enzymes OY
Inventors:
Marja Paloheimo, Leena Valtakari, Terhi Puranen, Kristiina Kruus, Jarno Kallio, Arja Mantyla, Richard Fagerstrom, Pentti Ojapalo, Jari Vehmaanpera
Abstract: The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium-mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.
Type:
Grant
Filed:
October 23, 2008
Date of Patent:
March 22, 2011
Assignee:
Unicrop Oy
Inventors:
Viktor Kuvshinov, Anne Kanerva, Kimmo Koivu, Svetlana Kuvshinova, Eija Pehu
Abstract: This disclosure provides a method and a product to treat fungal nail infections and Athlete's foot in a fast and non expensive way. The product contains one or more lytic peptides in water solution.
Abstract: This disclosure shows that the BPV-1 E2 protein-dependent minichromosome maintenance element (MME) comprised of E2 multimeric binding sites can provide the stable maintenance replication function to the mouse polyomavirus (PyV) core origin plasmids in the presence of BPV-1 E2 protein and PyV large T-antigen (LT). MME dependent plasmids are lost with the frequency of 6% per generation. Significantly long stable maintenance replication can also be provided without selection pressure. We also demonstrate that PyV core origin maintenance function/replication activation could be provided by Epstein-Barr virus Family of repeats and EBNA1 protein. The maintenance of the Polyomavirus core origin plasmid was characterized by 13% loss of the plasmid during one cell generation in the case of EBV FR harboring plasmids.
Type:
Grant
Filed:
February 10, 2006
Date of Patent:
September 7, 2010
Assignee:
Kosagen Cell Factory Oü
Inventors:
Toomas Silla, Ingrid Tagen, Jelizaveta Geimanen, Kadri Janikson, Aare Abroi, Ene Ustav, Mart Ustav, Tiiu Mandel
Abstract: The present invention is related to a recoverable block of function (RBF) system for controlling transgene escape and transgene containment in plants. The RBF-system comprises at least one blocking construct (BC), wherein the BC comprises as the blocking gene a nucleotide sequence or crtB gene encoding phytoene synthase, which is used alone or in combination with other BCs. The crtB gene expresses under the control of a germination specific promoter or a promoter constitutively repressed by a Recovering construct (RC) and blocks the germination of a transgenic seed. The blocked germination is recoverable by a user controlled intervention including addition of an effective amount of gibberellin and sugar and may involve so called recovering constructs (RCs), wherein the recovering gene may encode ent-copalyl diphosphate synthase or ent-kaurene synthase.
Type:
Grant
Filed:
July 5, 2005
Date of Patent:
September 7, 2010
Inventors:
Viktor Kuvshinov, Kimmo Koivu, Anne Kanerva, Andrei Anissimov
Abstract: An improved method for diagnosing autoimmune or genetic diseases is provided in this disclosure. In particular, a method to diagnose diseases affecting the tissues of organs selected from the group of spleen, brain, heart, kidney, thyroid, eye, skin, intestine, liver, pancreas, adrenal gland, prostate and lungs or from the tissues selected from the group of muscles and bones or other tissues. More specifically, the present invention provides an improved method for diagnosing diseases affecting neuromuscular junctions.
Type:
Grant
Filed:
March 14, 2008
Date of Patent:
August 17, 2010
Assignee:
University of Oulu
Inventors:
Anne Heikkinen, Hongmin Tu, Taina Pihlajaniemi
Abstract: The invention relates to genetic manipulation and evaluation of soybean seed, particularly for the enlargement and use of endosperm tissue for galactomannan gum extraction. A method for combining several elements which have heretofore been misunderstood or were unknown, is presented. The resulting enlarged endosperm tissue has many commercial uses.
Abstract: The present invention relates to novel laccase enzymes obtainable from the strains of the genus Thielavia or from the strains of the genus Chaetomium. The invention relates also to nucleic acid sequences encoding the enzymes, recombinant hosts into which the nucleic acid sequences have been introduced and to methods for the production of the enzymes in recombinant hosts. The enzymes of the invention are suitable for several applications, for example for treating denim and for strain removal.
Type:
Grant
Filed:
September 21, 2005
Date of Patent:
June 8, 2010
Assignee:
AB Enzymes Oy
Inventors:
Marja Paloheimo, Terhi Puranen, Leena Valtakari, Kristiina Kruus, Jarno Kallio, Arja Mantyla, Richard Fagerstrom, Pentti Ojapalo, Jari Vehmaanpera
Abstract: The present invention is related to a process based on a source-sink principle, for producing products of interest from crushed or uncrushed germinating dicotyledonous seeds comprising an expression system, which is induced or can be induced during germination. The product is either a seed derived composition comprising one or more gene products. Alternatively, it is a product of interest obtained by placing the composition in contact with a substrate, containing a substance capable of being transformed by the seed derived composition as such, dried or in down-stream processed form.
Type:
Grant
Filed:
October 18, 2007
Date of Patent:
June 1, 2010
Inventors:
Kimmo Koivu, Viktor Kuvshinov, Anne Kanerva, Andrei Anissimov, Seppo Paavo Kaijalainen
Abstract: A genotyping method and a prepared oligomicroarray as device is to determine single nucleotide polymorphism (SNP) and mutations are provided. The method uses two specific APEX-2 primers per each SNP or mutations to be determined. The same primers are used in amplification phase (primer extension and PCR with universal primer) and in the single base extension phase on an array. All SNP-containing sequences can be genotyped and amplified in one reaction tube and visualized on a microarray.
Abstract: A novel gene isolated from Deschampsia antarctica coding for a lipase like protein is disclosed. A system for production of recombinant enzyme is also disclosed as well as use for the enzyme in sunscreen.
Type:
Grant
Filed:
December 18, 2008
Date of Patent:
November 24, 2009
Assignees:
Vitrogen SA, Universidad de la Frontera
Inventors:
Manuel Gidekel, Ana Gutierrez, Claudia Rabert, Gustavo Gabrera, Jaime Sanchez, Leon Bravo
Abstract: Genetic mechanisms for mitigating the effects of introgression of a genetically engineered genetic trait of a cultivated crop to an undesirable, interbreeding related species.
Abstract: A novel gene isolated from Deschampsia antarctica coding for a lipase like protein is disclosed. A system for production of recombinant enzyme is also disclosed as well as uses for the enzyme.
Type:
Grant
Filed:
December 18, 2008
Date of Patent:
October 6, 2009
Assignees:
Vitrogen SA, Universidad de Concepcion
Inventors:
Manuel Gidekel, Ana Gutierrez, Claudia Rabert, Gustavo Gabrera, Jaime Sanchez, Leon Bravo
Abstract: The invention relates to isolated DNA sequences encoding MF3 polypeptides that induce multiple resistance in plants toward a variety of viral and microbial infections and against pests and a method of obtaining transgenic plants expressing MF3 polypeptides. The invention also relates to a method of isolating and purifying an MF3 polypeptide from bacterial cells expressing the polypeptide and its use as a plant protectant with or without carrier agent.
Type:
Grant
Filed:
December 17, 2004
Date of Patent:
September 22, 2009
Inventors:
Vitaly Dzhavakhia, Alexei Filippov, Konstantin Skryabin, Tatiana Voinova, Daria Shumilina, Ksenia Kromina, Maria Kouznetsova, Olga Shulga, Mikhail Pridanniko, Natalia Battchikova, Timo Korpela
Abstract: The present invention provides antimicrobials for efficient therapy of infections caused by bacterial pathogens. The antimicrobials include peptides corresponding to the active site of N-terminal extension of subunits composing specific adhesive organelles of virulence of pathogenic bacteria. These antimicrobial peptides inhibit surface expression of adhesive organelles and thus prevent development of infections caused by pathogenic bacteria. The invention describes also effective methods for screening molecules inhibiting the generation of the adhesive organelles.
Type:
Grant
Filed:
July 14, 2003
Date of Patent:
June 9, 2009
Inventors:
Timo Kalevi Korpela, Anton Zavialov, Vladimir Zav'yalov
Abstract: An improved double Recoverable Block of Function (RBF) system for transgene containment with enhanced reliability is disclosed. The system includes two blocking constructs (BCs) at the opposite sides of a transgene of interest (TGI). The system comprises means for recovery of the function blocked by expression of the blocking genes of the BCs. The invention is also related to a kit comprising a cloning vector having the BCs and TGI or alternatively a place for TGI to be inserted in. Segregating (two-insert) and inducible (one-insert) types of double RBF are disclosed. The two BCs are either similar or different in their DNA and RNA structures. The two BCs may encode same protein but promoters driving the blocking genes are different and/or the coding sequence of the blocking genes is modified by codon preference change. A system comprising two BCs having different blocking genes is also disclosed.
Type:
Grant
Filed:
July 15, 2004
Date of Patent:
February 24, 2009
Assignee:
Unicrop Ltd
Inventors:
Viktor Kuvshinov, Kimmo Koivu, Anne Kanerva, Andrei Anissimov
Abstract: A novel gene isolated from Deschampsia antarctica coding for a lipase like protein is disclosed. A system for production of recombinant enzyme is also disclosed as well as uses for the enzyme.
Type:
Grant
Filed:
April 16, 2007
Date of Patent:
February 3, 2009
Assignee:
Vitrogen S.A.
Inventors:
Manuel Gidekel, Ana Gutierrez, Claudia Rabert, Gustavo Gabrera, Jaime Sanchez, Leon Bravo
Abstract: A floating, waterproof, hanging eyewear holder designed as a convenient receptacle for showering, exercising, swimming, and other situations where eyewear can be easily stored and retrieved. A pocket is made of two thin layers of plastic, molded together to create an air space in the rear of the pocket. The third, front layer of the pocket is webbed to allow easy evaporation of any liquid. A snap secures eyewear in the pocket. A molded, offset top hanger allows convenient suspension from such surfaces as a shower curtain rod, tree branch, or exercise bar. The end of the hanger is bent inward to form a toe, allowing the hanger to be hung on any flat surface, such as a chair arm, table, or window sill. A hole in the top of the hook accommodates a lanyard or a suction cup, allowing the hanger to be attached to glass or ceramic enclosures.
Abstract: The present invention provides structures of small molecules capable of modulating apoptotic cell death. More specifically, the structures relate to the structures of apoptotic active sites of mammalian alpha-fetoprotein (AFP) and albumin. Peptides mimicking the active site contain two sequences, Arg-Gly-Asp and Asp-X-X-Asp, wherein X means any amino acid. These sequences are needed in the same molecule for causing a wide range of biological activities. The peptides can be utilized to suppress apoptotic pathways by inhibiting the cytochrome c-mediated caspase activation. Thus, the peptides can be used to inhibit effects of apoptosis induced by oxidative stress, drugs, cytokines, Fas-ligand, alpha-fetoprotein, used to prevent apoptosis in culturing cells, in organ transplantation, in immunological autoimmune disorders and immunodeficiency syndrome induced by viral infection, or to diminish side cytotoxic effects after chemotherapy and radiation therapy.
Type:
Grant
Filed:
October 7, 2003
Date of Patent:
November 4, 2008
Inventors:
Elena Ivanovna Dudich, Lidia Nikolaevna Semenkova, Igor Vyacheslavovitch Dudich, Edvward Borisovitch Tatulov, Dimitry Lvovicth Zubov, Timo Kalevi Korpela