Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a Bacillus cell, wherein the mutant (i) comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes responsible for the biosynthesis or secretion of a surfactin or isoform thereof under conditions conducive for the production of the polypeptide and (ii) the mutant produces less of the surfactin or isoform thereof than the Bacillus cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of Bacillus cells and methods for producing the mutants.

Abstract: The present invention relates to Aspergillus oryzae 5-aminolevulinic acid synthases and isolated nucleic acid fragments comprising nucleic acid sequences encoding the 5-aminolevulinic acid synthases as well as nucleic acid constructs, vectors, and recombinant host cells comprising the nucleic acid sequences. The invention also relates to methods of producing the 5-aminolevulinic acid synthases.

Abstract: The present invention relates to novel organic compounds, to methods for their preparation, to compositions containing them, to their use for treatment of human and animal disorders, to their use for purification of proteins or glycoproteins, and to their use in diagnosis. The invention relates to modulation of the activity of molecules with phospho-tyrosine recognition units, including protein tyrosine phosphatases (PTPases) and proteins with Src-homology-2 domains, in in vitro systems, microorganisms, eukaryotic cells, whole animals and human beings. The novel organic compounds are compounds of formula (I)(L).sub.n --Ar.sub.1 --R.sub.1 --A (I)wherein(L).sub.n, n, Ar.sub.1, R.sub.1 and A are as defined in the application.

Abstract: The present invention relates to methods for modifying the production of a polypeptide, comprising: (a) introducing a nucleic acid construct into a cell, wherein the cell comprises a DNA sequence encoding a polypeptide, under conditions in which the nucleic acid construct integrates into the genome of the cell at a locus not within the DNA sequence encoding the polypeptide to produce a mutant cell, wherein the integration of the nucleic acid construct modifies the production of the polypeptide by the mutant cell relative to the cell when the mutant cell and the cell are cultured under the same conditions; and (b) identifying the mutant cell with the modified production of the polypeptide.

Abstract: The present invention relates to injection syringes comprising a housing accommodating an ampoule containing medicine sufficient for a number of dosed injections. The syringe has a dose setting mechanism by which doses may be set by rotating a dose setting element relative to the housing and the dose set is indicated on a scale. The scale is formed as a clock dial having a first part secured to the housing and a second part which is rotatable relative to the first part and which is coupled to the dose setting element, one of parts carries the scale and the other carries an indicating member indicating a point on the scale. The angular distance between the divisions of the scale corresponds to the minute divisions on an ordinary clock. Holes are provided along the scale which holes can receive a pin forming a stop which cannot be passed by the indicating member.

Abstract: The present invention relates to novel proteolytic enzymes. More specifically, the present invention relates to proteolytic enzymes obtainable from strains of Amycolata and Amycolatopsis. Moreover the invention relates to a process for the preparation of the proteolytic enzyme of the invention, as well as detergent additives and detergent compositions comprising the proteolytic enzyme.

Abstract: The present invention relates to a gene encoding an ascomycete or deuteromycete carboxypeptidase Y gene, and host cells modified so as to produce reduced amounts of carboxypeptidase.

Abstract: Disclosed is a process for preparing (-)-3,4-trans-diarylchromans of formula I ##STR1## wherein R.sup.1, R.sup.2, R.sup.3, R.sup.4 and R.sup.5 are as defined in the specification. This process involves reacting a (+,-)-3,4-cis-diarylchroman with a chiral acid in an inert organic solvent to obtain the chiral acid salt of the (-)-3,4-cis enantiomer in crystalline form, subjecting the crystalline salt to hydrolysis, and rearranging the (-)-3,4-cis enantiomer to the (-)-3,4-trans enantiomer with a strong base in an inert aprotic solvent.

Abstract: The present invention relates to therapeutically active azacyclic compounds, a method of preparing the same and to pharmaceutical compositions comprising the compounds. The novel compounds are useful as stimulants of the cognitive function of the forebrain and hippocampus of mammals and especially in the treatment of Alzheimer's disease, severe painful conditions and glaucoma.

Abstract: The present invention relates to novel compounds of the general formula I ##STR1## which can be used for treating medical disorders resulting from a deficiency in growth hormone.

Abstract: This invention relates to an enzyme which exhibits cellulase activity and is producible by a strain of Bacillus sp., NCIMB 40250, or a related Bacillus sp. strain, or a derivative of said cellulase. This invention also relates to an enzyme which comprises a core region derived from an endoglucanase combined with a cellulose-binding domain derived from another cellulase, or which comprises a core region derived from another cellulase combined with a cellulose-binding domain derived from an endoglucanase.

Abstract: The present invention relates to novel N-substituted amino alcohols, amino acids and acid derivatives thereof in which a substituted alkyl chain forms part of the N-substituent or salts thereof, to methods for their preparation, to compositions containing them, and to their use for the clinical treatment of painful, hyperalgesic and/or inflammatory conditions in which C-fibers play a pathophysiological role by eliciting neurogenic pain or inflammation.

Abstract: This invention relates to novel stabilized enzymes. More specifically the invention relates to novel stabilized enzymes, in which a naturally occurring amino acid residue (other than proline) has been substituted with a proline residue at one or more positions; at which position(s) the dihedral angles .phi. (phi) constitute values within the interval ?-90.degree.<.phi.<-40.degree.!; preferably the dihedral angles .phi. (phi) and .psi. (psi) constitute values within the intervals ?-90.degree.<.psi.<-40.degree.! and ?-180.degree.<.psi.<-150.degree. or -80<$g c)<10 or 100<.psi.<180!; and which positions(s) is/are not located in regions in which the enzyme is characterized by possessing .alpha.-helical or .beta.-sheet structure. The invention also relates to nucleotide sequences encoding the novel stabilized enzymes, and expression vectors and host organisms containing the nucleotide sequences. This invention also relates to detergent compositions comprising the stabilized enzymes.

Abstract: The present invention relates to therapeutically active azatricyclic compounds of formula I ##STR1## a method of preparing the same and to pharmaceutical compositions comprising the compounds. The novel compounds are useful in treating a disease in the central nervous system caused by malfunctioning of the muscarinic cholinergic system.

Abstract: The invention is directed to a method of bleaching dye or colorant in solution which comprises a phenol oxidizing enzyme (e.g. A peroxidase or a laccase) and an enhancing agent (e.g. acetosyringone).

Abstract: The present invention relates to novel N-substituted amino alcohols, amino acids and acid derivatives thereof in which a substituted alkyl chain forms part of the N-substituent or salts thereof, to methods for their preparation, to compositions containing them, and to their use for the clinical treatment of painful, hyperalgesic and/or inflammatory conditions in which C-fibers play a pathophysiological role by eliciting neurogenic pain or inflammation.

Abstract: The present invention relates to appetite-suppressing peptides or an appetite-suppressing peptide-containing fraction for the treatment of obesity or type II diabetes.

Abstract: In a joint for joining a first and a second part so that these first and second parts maintain a predefined rotational position in relation to each other, where the joint comprises an internal thread (33) in the firs part (34), which internal thread has an inner end and an outer free end receiving an outer free end of a corresponding external thread (22) on the second part (21) which external thread has an inner end adjacent to the second part, the free end of the ridge and the inner end of the groove forming the threads are bounded by surfaces (27, 39) lying in a plane through the axis of the threads, and the length of the threads are so adapted that a plane end surface of the ridge of one thread engages the plane end surface of the groove of the other thread when the two parts are screwed together and occupy the predefined mutual rotational position.

Abstract: The present invention relates to a method for re-forming keratinous fibre cross links comprising contacting at least one oxidoreductase with the keratinous fibres for a sufficient period of time and under conditions sufficient to permit re-formation of keratinous fibre cross links. The method may be performed simultaneously with dyeing of said keratinous fibres. The invention also relates to the use at least one oxidoreductase for re-formation of keratinous fibre cross links.

Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) introducing into a respiratory-defective mutant of a cell (i) one or more first nucleic acid sequences which complement the respiratory defect and (ii) a second nucleic acid sequence which encodes the polypeptide; (b) cultivating the cell containing the first and second nucleic acid sequences in a culture medium under aerobic conditions suitable for expression of the first and second nucleic acid sequences; and (c) isolating the polypeptide from the cultivation medium of the cell. The present invention also relates to methods for disrupting a gene in a respiratory-deficient mutant cell. The present invention further relates to respiratory-deficient mutant cells and methods for obtaining such mutant cells.