Abstract: The present invention is based on the discovery of a simplified assay for identifying modulators of ubiquitin ligase activity. This assay allows detection of compounds that affect ubiquitination and thus, cell cycle regulation in cells. An increase in ubiquitination, in comparison to a test sample lacking a test compound, indicates a stimulation of activity, whereas a reduction in ubiquitination indicates an inhibitor of activity. Also disclosed herein are methods of identifying proteins having ubiquitin ligase activity, methods of identifying substrates for ubiquitination, methods for identifying an activity relationship between a particular ubiquitin ligase and a particular ubiquitin conjugating enzyme, and chimeric proteins comprising a ubiquitin conjugating enzyme and a ubiquitination substrate, which are useful in all of the disclosed methods.

Abstract: This invention provides an optical probe useful as an optical probe or sensor of post translational type modifications, such as phosphorylation. The invention comprises a polypeptide moiety, which contains a recognition motif for a post translational type activity and a protease site, which is coupled to a probe moiety. Modification of the polypeptide, by the post translational type activity, results in a modulation of the rate at which a protease cleaves the polypeptide which is sensed by a measurable change in at least one optical property of the optical probe upon cleavage. The present invention also includes a recombinant nucleic acid molecule that encodes an optical probe and a vector and host cell or library of cells that include the recombinant nucleic acid molecule. The optical probe can be used in methods to determine whether a sample, including a cell or a sample from an organism, contains a post-translational type modification activity.

Abstract: Catalase enzymes derived from bacteria from the genera Alcaligenes (Deleya) and Microscilla are disclosed. The enzymes are produced from native or recombinant host cells and can be utilized to destroy or detect hydrogen peroxide, e.g., in production of glyoxylic acid and in glucose sensors, and in processes where hydrogen peroxide is used as a bleaching or antibacterial agent, e.g. in contact lens cleaning, in bleaching steps in pulp and paper preparation and in the pasteurization of dairy products.

Abstract: A nonvolatile semiconductor memory includes a plurality of memory cells arranged in columns and rows, a plurality of word lines, a plurality of bit lines, a plurality of output buffers, a plurality of page latches 18L, and a plurality of Quick Current Level Translators (QCLT). Each QCLT is connected to and is shared by a plurality of bit lines (32 in the preferred embodiment) through a first column decoder 44/46U and is also connected to a plurality of page latches through a second column decoder 46L. Each page latch is connected to one corresponding output buffer through a third column decoder circuit 38/40/42. The page latches are grouped in a plurality of sub-pages. The QCLT performs high speed and high accuracy current-mode comparison and converts the result of comparison into binary codes. These codes are stored in Q-latches 36U-2. The QCLT functions as a current-mode analog-to-digital converter (ADC) which converts the memory cell current to binary codes.

Abstract: The invention relates to a method for transferring, for instance, a biological material arranged in a given pattern, whereby the biological material is brought into contact with needles placed on the head of a robot and the biological material is transferred to a support, whereby the needles are hard metal needles fitted with a biocompatible coating. The biocompatible coating preferably consists of metal-nitrogen compounds. Preferably, an anticorrosion coating is applied underneath the biocompatible coating. Once the biological material has been arranged in a given pattern, the needles mounted on the robot head are arranged according to the same pattern. Preferably, the pattern corresponds to the pattern of the arrangement of microtitre plate wells. The invention also relates to a robot head fitted with the inventive hard metal needles. Said robot head particularly forms part of a picking and/or a spotting robot.

Abstract: In the present invention a second pole yoke component of a second pole piece is connected (stitched) to a small top surface of a second pole tip component of the second pole piece as well as to a back edge and first and second side edges of the second pole tip component. The extra stitching at the back and side edges provides the required magnetic coupling between the components for transferring flux to and from a second pole tip at an air bearing surface. This arrangement shortens the length of the top stitched area so that one or more coil layers in an insulation stack can be located closer to the ABS for increasing the data rate of the head. Further, this arrangement reduces the amount of material of the second pole piece layer in close proximity to the first pole piece layer so as to minimize flux leakage therebetween.

Abstract: A support assembly including first and second mounting brackets each having a mounting surface for attachment to a bottom surface of a desktop, first and second support arms pivotally attached to the first and second mounting brackets respectively to define first and second pivot axes, and a computer keyboard platform having a top support surface and a bottom surface. The first and second support arms rotate in a single plane about the first and second pivot axes respectively. The first and second support arms are pivotally attached to the bottom surface of the platform to define third and fourth pivot axes respectively. The support arms are rotatable relative to the platform in the single plane about the third and fourth pivot axes respectively.

Abstract: A method and device to generate behavior for emulated visitors traversing an internet web site. The visitors may display behavior that is indistinguishable from those of actual users, a subset of the actual users, or the behavior may be purely hypothetical, such as when a visitor acts without evidence of having made an intentional choice. The invention tracks the actions of the visitors and develops reference distributions that may be compared to a site's usage distributions as obtained from actual visitors to the site. The reference distributions are then used to implement statistical estimation methods that measure relative information content. The invention comprises a general implementation and a deterministic implementation. The general version may be applied to live production web sites, and the deterministic version is best suited to offline processing.

Abstract: In a two-clock charge pump an improved output stage minimizes the voltage swing at the output node. The output stage has a first and second MOS transistors whose first terminals are connected together to receive the input signal. The second terminal of the first MOS transistor is connected to the gate of the second MOS transistor. The second terminal of the second MOS transistor is connected to the output node. A first and second clock signals are supplied to the gates of the first and second MOS transistors. Diode means in the nature of an MOS transistor having a gate connected to one of its terminals are provided connecting the output node to the gate of the first MOS transistor.

Abstract: This invention provides assays for protein kinase activity using fluorescent proteins engineered to include sequences that can be phosphorylated by protein kinases. The proteins exhibit different fluorescent properties in the non-phosphorylated and phosphorylated states.

Abstract: A non-contact wound covering for covering a wound. A peripheral sealing ring is covered by a barrier layer and this assembly is attached to the skin with an adhesive. The barrier layer and peripheral sealing ring together define a treatment volume over the wound. The barrier layer may include active and passive heaters and the sealing ring may dispense water to control the humidity of the treatment volume. One form of active heat is the transfer of a heated fluid to the wound covering.

Abstract: A write head has a second pole tip layer, a coil layer and a write coil insulation layer that are planarized at their top surfaces. A thin top insulation layer insulates the top of the coil layer from a yoke portion of the second pole piece which is connected to the second pole tip layer in the pole tip region and connected to a first pole piece layer in a back gap region. In a preferred embodiment the write gap layer extends throughout the yoke region and provides the only insulation between the first pole piece layer and the coil layer. Further, it is preferred that the write coil insulation layer be an inorganic material such as silicon dioxide (SiO2). Several embodiments of the write head are provided along with novel methods of making.

Abstract: An efficient method and apparatus for regulating access to information objects stored in a database in which there are a large number of users and access groups. The invention uses a representation of a hierarchical access group structure in terms of intervals over a set of integers and a decomposition scheme that reduces any group structure to ones that have interval representation. This representation allows the problem for checking access rights to be reduced to an interval containment problem. An interval tree, a popular data structure in computational geometry, may be implemented to efficiently execute the access-right checking method.

Abstract: A dual GMR or dual spin valve sensor has a self-pinned layer which has its magnetic moment pinned perpendicular to an air bearing surface by sense current fields from conductive layers in the dual spin valve sensor when a sense current is conducted therethrough. This scheme eliminates one of the antiferromagnetic pinning layers which is typically employed in a dual GMR or dual spin valve sensor. The self-pinned layer is thin so that its demagnetization field will not be greater than the sense current fields acting thereon. Because of the thinning of the self-pinned layer the spin valve effect of the spin valve sensor is degraded by scattering of conduction electrons at the boundary of the self-pinned layer.

Abstract: A method makes a magnetic head at first, second, third, fourth and fifth regions on a substrate including forming a read sensor material layer in all regions, forming a first capping layer on the read sensor material layer in all regions, removing the first capping layer and the sensor material layer in all regions except the first, second and third regions wherein the first region is definable by a read sensor and the second and third regions abut opposite side edges of the first region, forming a low resistance material lead layer and a second capping layer in the fourth and fifth regions wherein the fourth and fifth regions abut the second and third regions respectively, selectively removing portions of the first capping layer in the second and third regions, selectively removing portions of the read sensor material layer in the second and third regions and portions of the second capping layer in the fourth and fifth regions and forming a high resistance material lead layer in the second, third, fourth and

Abstract: A multi-zone high-density inductively-coupled plasma source includes a first individually controlled RF antenna segment for producing a plasma from a process gas. A second individually controlled coil segment is included in the ICP source for producing a plasma from a process gas. In various embodiments, more than two sets of individually controlled coil segments may be used. In one embodiment, a separate power supply may be used for each coil segment individually. Another aspect of this invention is a hermetically-sealed inductively-coupled plasma source structure and method of fabrication which eliminates the possibility of process contamination, improves the source hardware reliability and functionality, and improves the vacuum integrity and ultimate base pressure of the plasma system.

Abstract: Fluorescent indicators including a binding protein moiety, a donor fluorescent protein moiety, and an acceptor fluorescent protein moiety are described. The binding protein moiety has an analyte-binding region which binds an analyte and causes the indicator to change conformation upon exposure to the analyte. The donor moiety and the acceptor moiety change position relative to each other when the analyte binds to the analyte-binding region. The donor moiety and the acceptor moiety exhibit fluorescence resonance energy transfer when the donor moiety is excited and the distance between the donor moiety and the acceptor moiety is small. The indicators can be used to measure analyte concentrations in samples, such as calcium ion concentrations in cells.

Abstract: A magnetic head is provided that has an extremely small first shield layer and first and second leads of a read head that extend from a magnetoresistive (MR) sensor to first and second pads without any vias therebetween. This is accomplished by patterning the first shield layer into its final shape before the first gap layer and subsequent layers are formed. An alumina mask with a recess at the ABS of the size of the first shield layer is formed followed by forming the first shield layer in the recess. A very thin first gap layer and an optional supplemental first gap layer are then formed, the supplemental first gap layer insulating any potential exposure of the first shield layer at its junction with the alumina layer. After constructing the MR sensor, first and second leads for the MR sensor are constructed from the MR sensor to sites of the first and second pads.

Abstract: A plug-in connection is described for the joining of pipelines and hose lines, especially for use with pipeline systems of motor vehicles. The plug-in connection consists of an inner sleeve and an outer sleeve, which hold a hose line between them, so that a pipe socket can be shoved across the outer sleeve, which can be locked to the outer sleeve by means of a protective element. Inner sleeve and outer sleeve accommodate a sealing means which, supported by the pressure force of the pressurized medium acting on the inner sleeve, is pressed against the inner wall of the pipe socket.

Abstract: A magnetic head is provided which has first and second notches in a first pole piece layer adjacent first and second corners at the base of a gap layer wherein the gap layer does not undercut a base of a second pole tip. Field regions of the first pole piece layer which extend from the first and second notches slope upwardly from the notches for protecting components below the first pole piece layer. In one aspect of the invention a method of defining the gap layer sputter deposition and ion milling steps are employed to form a protective layer on first and second side walls of the second pole tip so that unwanted portions of the gap layer can be removed without undercutting the gap layer below the base of the second pole tip.