Abstract: A method for isolating and cloning methanol inducible genes from Pichia is taught. The regulatory regions are useful for the methanol regulated expression of heterologous genes.

Abstract: Single cell protein (SCP) is produced at high yields from a novel methanol assimilating Methylomonas sp. grown at elevated temperatures in an aerobic bacterial fermentation process.

Abstract: A method for purifying recombinant tumor necrosis factor from cultures of microorganisms transformed with vectors that code for the production of tumor necrosis factor is disclosed. A sequence of contacting steps is involved, such that the effluent from the first contacting step can be contacted directly with the contacting material used for the second step and similarly, the effluent from the second contacting step can be contacted directly with the contacting material used for the third contacting step. In a preferred embodiment, tumor necrosis factor-containing fermentation broth can be substantially purified by a single pass through a multi-component chromatography system, i.e., the first, second and third contacting materials are each packed in a column configuration, with TNF-containing broth being passed directly through the sequence of columns.

Abstract: Novel alumina-supported nickel boride catalysts prepared by the borohydride promoted reduction of nickel arsenate are described. Method of catalyst preparation and process for hydrogenation of acetylenic and diolefinic compounds employing the invention catalysts are also described.

Abstract: A drilling fluid additive is disclosed which will prevent water loss into subterranean formations during the drilling of oil and gas wells in hard brine environments. The drilling fluid additive comprises a hydrocarbon, an anionic surfactant, an alcohol, a sulfonated asphalt, a biopolymer, and optionally an organophilic clay, poly(N-vinyl-2-pyrrolidone-co-sodium-2-acryamido-2-methylpropane sulfonate, and water.

Abstract: Alcohol oxidase is rendered storage stable in dried form by the addition thereto of small amounts of at least one of peroxidase, catalase, hemoglobin, cytochrome c and myoglobin.

Abstract: Spray reduction means for moving vehicles are provided comprising at least one layer of non-woven fabric bonded to the surface of a backing containing channel means for draining water from the spray reduction means, said backing being capable of supporting the non-woven fabric. The spray reduction means is in the form of a laminate.

Abstract: Process for the selective hydrogenation of diolefinic compounds to monoolefinic compounds employing catalyst consisting essentially of elemental nickel on an inorganic support in the presence of hydrogen and at least one nitrogen-containing compound is disclosed. Selective hydrogenation of the less substituted of the two carbon-carbon double bonds of the diolefinic compound is achieved while isomerization of the more highly substituted, non-hydrogenated double bond, is minimized.

Abstract: An improved olefin disproportionation catalyst produced by contacting (a) molybdenum or tungsten oxide supported on inorganic refractory oxide, and (B) a metal dithionite.

Abstract: A process for producing a novel-highly active lanthanide containing catalyst comprising the product produced by reacting a lanthanide hydride, a Lewis acid, and an electron donor ligand with an organoaluminum cocatalyst component and its use in the polymerization of olefins, especially olefins such as ethylene and 1,3-butadiene.

Abstract: A highly active catalyst suitable for the polymerization of olefins and its use are disclosed, said catalyst being prepared by(1) co-comminuting an aluminum halide; at least one electron donor; a Group IVB-VIB transition metal compound; and a support base selected from the group consisting of the Group IIA and IIIA salts and the salts of the multivalent metals of the first transition series with the exception of copper to produce a coground solid;(2) extracting said coground solid with an organic liquid; and(3) separating the solid from the liquid under such conditions that at least 5 weight percent of the aluminum in the coground solid is removed.

Abstract: A water permeability contrast correction process to improve the sweep efficiency of waterflooding which involves the sequential injection of (1) an optional aqueous preflush slug to adjust connate water salinity, (2) an aqueous thickened slug comprising a sequestered polyvalent metal cation such as aluminum citrate and a gelable polymeric viscosifier such as polyacrylamide, (3) carbon dioxide to decrease the pH of the polymer slug which triggers the delayed in-situ gelation of said thickened slug to preferentially decrease water permeability in highly permeable thief zones, and (4) an aqueous drive fluid.

Abstract: A corrosion-inhibiting composition and method of inhibiting corrosion on metal surfaces are described. The composition comprises a reaction product of (a) a first constituent selected from an epoxide having an average of at least about one vicinal epoxide per molecule and a halogenated aliphatic compound having at least one displaceable halogen and (b) a second constituent comprising a dimercaptan. The reaction product in a hydrocarbon diluent is an effective corrosion inhibitor when applied to metal surfaces in highly corrosive environments, and is designed particularly for downhole metal surfaces of oil drilling equipment.

Abstract: A water permeability contrast correction process to improve the sweep efficiency of waterflooding in carbonate-bearing strata which involves a sequential injection of (1) an optional aqueous preflush slug to adjust connate water salinity, (2) a slug of aqueous polymer gelable with polyvalent metal cations, such as an polyacrylamide, (3) an acidic aqueous solution effective to dissolve some of the carbonate and at least therefrom polyvalent metal cations specifically calcium or magnesium or both effective to gel said polymer, wherein said aqueous polymer and acidic solution can be injected together in a single slug, followed by (4) an additional aqueous polymer solution injection, wherein the acidic solution provides polyvalent metal cations in-situ for gelation of said polymer slug to preferentially decrease water permeability in highly permeable thief zones, and (5) an aqueous drive fluid.

Abstract: A water permeability correction process, and composition therefor, to improve the sweep efficiency of waterflooding which involves the sequential injection of (1) an optional aqueous preflush slug to adjust connate water salinity, (2) an aqueous sequestered polyvalent metal cation, such as chromium, wherein the sequestering anion is bactericidal, such as propionate, (3) a gelable polymeric viscosifier, such as polyacrylamide, and, preferably, (4) an aqueous drive fluid.

Abstract: A new alcohol oxidase is isolated from Pichia-type microorganisms in soluble or crystalline form. The crystalline novel enzyme is isolated by preparing an aqueous fluid containing cells of a Pichia-type microorganism, homogenizing the fluid and separating solids therefrom to produce an alcohol oxidase solution, adjusting the solution to have an ionic strength in the range of 0.05 to 0.01 in ionic strength to form a recovery range solution thereby causing the crystalline alcohol oxidase to form. The new enzyme is used to determine alcohol concentrations in fluid samples in which conditions are compatible with the enzyme's activity.

Abstract: Method and apparatus for the separation of solid particulate polymer from liquid diluent comprising the use of one or more accumulator legs to which diluent is supplied, and separated diluent is recycled to the polymerization process.