Patents Represented by Attorney Jerome L. Jeffers

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  • Process for the production of solution stable alpha-amylase and liquid alpha-amylase produced thereby
    Patent number: 4724208
    Abstract: This invention contemplates a novel process for the preparation of solution stable alpha-amylase obtained from Bacillus licheniformis, and to the high potency liquid enzyme product prepared by this process. Typically, the enzyme-containing solution from a fermentation is concentrated and starch is added to the concentrate. Alternatively, a precipitation agent such as salt is added to the solution, and a cake containing the enzyme precipitates. The cake is then contacted with an aqueous solution containing starch to extract the enzyme out of the cake to provide a stable liquid enzyme product.
    Type: Grant
    Filed: November 4, 1985
    Date of Patent: February 9, 1988
    Assignee: Miles Laboratories, Inc.
    Inventors: Jack W. Brewer, Chong Y. Kim, Curtis J. Montgomery, Jayarama K. Shetty
  • Method for increasing the milk clotting activity of thermolabile rhizomucor pusillus rennet
    Patent number: 4722900
    Abstract: Disclosed is a method for increasing the milk clotting ability and reducing the thermal stability of microbial rennet from Rhizomucor pusillus. The method involves treating the rennet with a methionine oxidizing composition and the anhydride of a dicarboxylic organic acid.
    Type: Grant
    Filed: January 17, 1986
    Date of Patent: February 2, 1988
    Assignee: Miles Laboratories, Inc.
    Inventors: Herbert J. Havera, John D. Humphreys
  • Process for preserving acid producing bacteria and compositions produced thereby
    Patent number: 4720460
    Abstract: Described is a method to stabilize or preserve acid-producing bacteria in a buffered solution. A carrier may be added that is not a specific substrate for the bacteria in order to give texture, most preferably the carrier being polyethylene glycol or sodium alginate. Optionally, other ingredients such as anti-oxidants, mold inhibitors, and/or salts may be added. These compositions may be stored at temperatures above freezing for a time from a few days up to about 2 months or longer.
    Type: Grant
    Filed: April 4, 1985
    Date of Patent: January 19, 1988
    Assignee: Miles Laboratories, Inc.
    Inventors: Jeffrey T. Barach, Bassie J. Kamara
  • Thermal stabilization of alpha-amylase
    Patent number: 4717662
    Abstract: Disclosed is a method for enhancing the thermal stability of microbial alpha-amylase. The method involves adding a stabilizing amount of an amphiphile to the enzyme in its aqueous solution. Also included within the scope of the invention is the stabilized alpha-amylase formulation and its use in the liquefaction of starch.
    Type: Grant
    Filed: August 22, 1985
    Date of Patent: January 5, 1988
    Assignee: Miles Laboratories, Inc.
    Inventors: Curtis J. Montgomery, Jayarama K. Shetty, Eric C. Singley
  • Recovery of microbial lipase
    Patent number: 4717665
    Abstract: Disclosed is a method for causing the dissociation of microbial mycelium and extracellular lipase bound thereto and increasing the measurable activity of the lipase. The method involves treating an aqueous suspension of the mycelium with the anhydride of a dicarboxylic acid which results in dissociation of the mycelium and lipase thereby facilitating recovery of the lipase.
    Type: Grant
    Filed: May 16, 1986
    Date of Patent: January 5, 1988
    Assignee: Miles Laboratories, Inc.
    Inventors: Robert L. Charles, Steven C. Dolby, Chimanbhai P. Patel, Jayarama K. Shetty
  • Immobilization of biocatalysts on granular diatomaceous earth
    Patent number: 4713333
    Abstract: Disclosed is a process whereby enzymes are immobilized on granular diatomaceous earth. The process involves treating the diatomaceous earth with a polyamine compound having pendant amino groups to cause the polyamine to adhere to the diatomaceous earth leaving pendant amine groups free to react further. The free amine groups are derivatized by treatment with a difunctional compound having amine reactive moieties, so that free amine groups of an enzyme or enzymes can be covalently bound to the polyamine via the amine reactive compound.
    Type: Grant
    Filed: September 23, 1985
    Date of Patent: December 15, 1987
    Assignee: Miles Laboratories, Inc.
    Inventors: John P. Chiang, Oreste J. Lantero, Jr.
  • Composition for accelerating cheese aging
    Patent number: 4707364
    Abstract: Aging of cheese is accelerated by adding during cheesemaking a composition containing preserved, partially disrupted L. casei and/or L. lactis, and a dried lipase similar to kid or calf pre-gastric lipase. The L. casei or L. lactis has been preserved by freezing, freeze drying, spray drying or fluidized bed drying. Preferably, the L. casei is ATCC 39539 and the L. lactis is ATCC 39538. The composition may optionally contain a similarly preserved and partially disrupted L. plantarum, and/or a microbial neutral protease. The composition may be added to cheese milk before adding coagulant to coagulate the milk or to curd with salt before pressing.
    Type: Grant
    Filed: November 1, 1985
    Date of Patent: November 17, 1987
    Assignee: Miles Laboratories, Inc.
    Inventors: Jeffrey T. Barach, Larry L. Talbott
  • Method of preparing novel thermostable transglucosidase
    Patent number: 4689296
    Abstract: Disclosed is a method for preparing transglucosidase comprising culturing a strain of Talaromyces duponti in a suitable nutrient growth medium and isolating the transglucosidase therefrom. Also disclosed is the transglucosidase produced by T. duponti.
    Type: Grant
    Filed: September 23, 1985
    Date of Patent: August 25, 1987
    Assignee: Miles Laboratories, Inc.
    Inventors: John P. Chiang, Oreste J. Lantero, Jr.
  • Dust free particulate enzyme formulation
    Patent number: 4689297
    Abstract: Dust free enzyme containing particles are produced by coating hydratable core particles with an enzyme and then with a film-forming macro-molecular material. Coating is carried out by suspending the core particles in a fluidized bed dryer, spraying an aqueous slurry of enzyme onto the core particles while suspended, and evaporating water to leave a dried enzyme coat on the particles. The resultant enzyme-coated particles, while still suspended in the fluidized bed, are sprayed with a solution or dispersion of the macro-molecular material, and dried to remove solvent to leave a coating of the macro-molecular material.
    Type: Grant
    Filed: March 5, 1985
    Date of Patent: August 25, 1987
    Assignee: Miles Laboratories, Inc.
    Inventors: Ivan C. Good, Yun C. Jao
  • Caustic based cleaning composition containing THFA and a propoxylated alcohol or phenol
    Patent number: 4686065
    Abstract: Disclosed is a cleaning composition for removing baked on soils from hard surfaces. The composition comprises an alkali metal hydroxide, and a solvent system made up of tetrahydrofurfuryl alcohol together with a propoxylated alcohol or phenol and a thickener to provide a composition having a suitable viscosity for its intended use.
    Type: Grant
    Filed: May 23, 1986
    Date of Patent: August 11, 1987
    Assignee: Miles Laboratories, Inc.
    Inventors: Kenneth E. Bliznik, Gilles M. L. Verboom
  • Method for the purification of calf rennet
    Patent number: 4666843
    Abstract: Disclosed is a method for the purification of calf rennet which involves contacting the rennet with an affinity ligand, e.g. Cibacron Blue, to cause separation of the chymosin and pepsin contained in the rennet.
    Type: Grant
    Filed: October 16, 1985
    Date of Patent: May 19, 1987
    Assignee: Miles Laboratories, Inc.
    Inventor: Sethuraman Subramanian
  • Process to recover crystalline enzymes and crystalline enzymes produced thereby
    Patent number: 4659667
    Abstract: This invention relates to a novel process for the recovery of enzyme crystals. The enzymes may be obtained from any enzyme-producing microorganisms such as bacteria, fungi, and yeasts. The invention contemplates supersaturation and/or crystallization to obtain enzymes in the crystalline form, and is particularly effective for the recovery of heat stable alpha-amylase in a crystal form.
    Type: Grant
    Filed: May 20, 1985
    Date of Patent: April 21, 1987
    Assignee: Miles Laboratories, Inc.
    Inventors: Jack W. Brewer, Chong Y. Kim
  • Method to make effervescent calcium tablets and calcium tablets produced thereby
    Patent number: 4650669
    Abstract: Described is a directly compressible formulation comprising calcium carbonate, citric acid, and a compression vehicle. In the preferred embodiment, the compression vehicle comprises a maltodextrin and lactose. The formulation can be compressed into an effervescent tablet system, which when placed in water effervesces in a relatively rapid dissolution releasing CO.sub.2 and resulting in a solution of monocalcium citrate.
    Type: Grant
    Filed: July 30, 1985
    Date of Patent: March 17, 1987
    Assignee: Miles Laboratories, Inc.
    Inventors: Thomas A. Alexander, Donald L. Peterson
  • Method of biopolymeric sludge dewatering
    Patent number: 4609470
    Abstract: An improved method for dewatering aqueous sludge is disclosed comprising flocculating the sludge by the addition of effective amounts of chitosan and an organic dialdehyde such as glutaraldehyde to the sludge, which is also preferably acidified to about pH 6.0.
    Type: Grant
    Filed: July 8, 1985
    Date of Patent: September 2, 1986
    Assignee: Miles Laboratories, Inc.
    Inventors: Koo-Heung Chung, Gary L. Davis, Francis H. Verhoff
  • Two component stain composition for producing a Giemsa blood stain effect
    Patent number: 4595524
    Abstract: Disclosed is a novel, 2 component Giemsa stain. The stain comprises a first component which is an aqueous solution of methylene blue, Azure B and a preservative and a second component which is an acetone/dimethyl formamide/water solvent system containing the sodium salt of eosin Y and a buffer. The Giemsa stain effect is obtained by first fixing a blood slide with methanol and then applying the freshly mixed first and second components of the stain. The slide is then rinsed and dried to prepare it for examination.
    Type: Grant
    Filed: March 28, 1984
    Date of Patent: June 17, 1986
    Assignee: Miles Laboratories, Inc.
    Inventors: Kin F. Yip, Frances F. Rhodes
  • Method for determination of transglucosidase
    Patent number: 4575487
    Abstract: Disclosed is a method for the determination of transglucosidase activity in glucoamylase preparations suspected of having TG activity. The method involves inhibiting the glucoamylase in the preparation with acarbose and then measuring the uninhibited TG activity by conventional means.
    Type: Grant
    Filed: May 25, 1983
    Date of Patent: March 11, 1986
    Assignee: Miles Laboratories, Inc.
    Inventors: Jayarama K. Shetty, J. John Marshall
  • Acid soluble annatto colorant in a powdered form
    Patent number: 4548822
    Abstract: Disclosed is a method for the preparation of an acid soluble annatto colorant in a powdered form and the annatto colorant prepared by this process. The method involves mixing an alkaline soluble annatto extract with an aqueous dispersion of a dextrinized starch derivative and drying the resultant to provide the desired product.
    Type: Grant
    Filed: May 2, 1984
    Date of Patent: October 22, 1985
    Assignee: Miles Laboratories, Inc.
    Inventor: Thomas R. Schmidt
  • Conjugate addition of organocuprates generated from copper (I) cyanide and vinyl stannanes useful in prostaglandin analog synthesis
    Patent number: 4543421
    Abstract: An improved method for the preparation of prostaglandin analogs which comprises preparation of two equivalents of an alkyl or alkenyl lithium reagent in an aprotic solvent mixture such as pentane or hexane and tetrahydrofuran, conversion of said reagent by addition of 1 equivalent of copper (I) cyanide to a copper reagent, conversion of said copper reagent to the blocked prostaglandin analog by the addition of a 2-alkylcyclopent-2-en-1-one reagent and quenching of the resultant reaction mixture with aprotic media or an active acylating agent and finally optionally deblocking the resultant product is disclosed. This method is an improvement over previous methods in that more modest temperatures and less hazardous and/or less complex reagents are used and in that for some analogs the method is shorter than previous methods.
    Type: Grant
    Filed: May 11, 1983
    Date of Patent: September 24, 1985
    Assignee: Miles Laboratories, Inc.
    Inventors: Paul F. Corey, Harold C. Kluender
  • Method for the preparation of spherical microorganism cell aggregates
    Patent number: 4543332
    Abstract: Spherically shaped bacterial cell aggregates are produced by spheronizing extruded flocculated cells. The cells are flocculated from aqueous medium with a cross-linked polyamine which is the reaction product of an epihalohydrin/polyamine copolymer and a cross-linking agent. Prior to being extruded, a filter cake having 68 to 76 weight percent water is produced by filtration of the flocculated cells, and the filter cake is ground into particles no greater than 60 mesh. Spheronizing is with a plate rotating at a tangential velocity of 4.5 to 12 meters per second within a cylinder containing the plate. Toughness of the spherical aggregates produced can be increased by the addition of a binder after filtration and before extrusion. During spheronizing, fines may be produced. These fines can be recycled by mixing them with the wet filter cake and binder before extrusion.
    Type: Grant
    Filed: February 24, 1983
    Date of Patent: September 24, 1985
    Assignee: Miles Laboratories, Inc.
    Inventors: Yun C. Jao, Ivan C. Good
  • Device and method for the quantitative determination of heparin in mammalian blood plasma
    Patent number: 4543335
    Abstract: A device for the quantitative determination of heparin in mammalian blood plasma, a method for the preparation of such device and a method for determining the plasma's heparin concentration using the device. The device, which is prepared using a freeze-drying technique, comprises a first upper layer of a carrier matrix material containing a buffer, AT-III and thrombin and a second, lower layer of a carrier matrix containing a thrombin sensitive fluorogenic or chromogenic substrate being capable of interacting with thrombin in such a manner that a time-related chemical change detectable by fluorometric or spectrophotometric means takes place when thrombin and the substrate are contacted in a suitable liquid environment.
    Type: Grant
    Filed: December 20, 1982
    Date of Patent: September 24, 1985
    Assignee: Miles Laboratories, Inc.
    Inventors: Ronald G. Sommer, Alfred C. Greenquist