Patents Represented by Attorney Kirk Ekena

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  • Endosomolytic polymers
    Patent number: 8217015
    Abstract: We describe pH-sensitive endosomolytic polymers, delivery particles containing pH-sensitive endosomolytic polymers. The described particles are capable of delivering polynucleotides to cells from the peripheral circulation with subsequent release from endosomes. The endosomolytic polymers are inactive outside the cell but disrupt membranes upon exposure to an acidified endosomal compartment.
    Type: Grant
    Filed: April 1, 2004
    Date of Patent: July 10, 2012
    Assignee: Arrowhead Madison Inc.
    Inventors: David B. Rozema, Darren Wakefield
  • Endosomolytic polymers
    Patent number: 8211468
    Abstract: We describe pH-sensitive endosomolytic polymers, delivery particles containing pH-sensitive endosomolytic polymers. The described particles are capable of delivering polynucleotides to cells from the peripheral circulation with subsequent release from endosomes. The endosomolytic polymers are inactive outside the cell but disrupt membranes upon exposure to an acidified endosomal compartment.
    Type: Grant
    Filed: September 19, 2006
    Date of Patent: July 3, 2012
    Assignee: Arrowhead Madison Inc.
    Inventors: David B. Rozema, Darren H. Wakefield, Jon A. Wolff, James E. Hagstrom, Kirk Ekena
  • Membrane active heteropolymers
    Patent number: 8138383
    Abstract: The present invention is directed to a class of membrane active polymers useful for cellular delivery of compounds. Conjugation of the described membrane active polymers to targeting, anti-opsonization, and anti-aggregation agents provides polymers suitable for in vivo delivery. The use of multiple reversible linkages connecting component parts provides for physiologically responsive activity modulation.
    Type: Grant
    Filed: May 19, 2008
    Date of Patent: March 20, 2012
    Assignee: Arrowhead Madison Inc.
    Inventors: Darren H. Wakefield, David B. Rozema, Jon A. Wolff, James E. Hagstrom
  • Polyconjugates for in vivo delivery of polynucleotides
    Patent number: 8137695
    Abstract: The present invention is directed to compounds, compositions, and methods useful for delivering polynucleotides or other cell-impermeable molecules to mammalian cells. Described are polyconjugates systems that incorporate targeting, anti-opsonization, anti-aggregation, and transfection activities into small biocompatible in vivo delivery vehicles. The use of multiple reversible linkages connecting component parts provides for physiologically responsive activity modulation.
    Type: Grant
    Filed: August 17, 2007
    Date of Patent: March 20, 2012
    Assignee: Arrowhead Madison Inc.
    Inventors: David B. Rozema, James E. Hagstrom, Jason Klein, David L. Lewis, Sean D. Monahan, Darren H. Wakefield, Jon A. Wolff, So Wong
  • Methods for expression of transgenes
    Patent number: 8021875
    Abstract: The present invention relates to compositions and methods for increasing long term expression in vitro and in vivo, comprising sequences and gene expression cassettes that increase expression of genes to which they are operably linked.
    Type: Grant
    Filed: August 27, 2002
    Date of Patent: September 20, 2011
    Assignee: Roche Madison Inc.
    Inventors: Christine I. Wooddell, Hans Herweijer
  • Endosomolytic poly(acrylate) polymers
    Patent number: 8017109
    Abstract: The present invention is directed to membrane active polymers useful for cellular delivery of compounds. Described are polyconjugates systems that incorporate targeting, anti-opsonization, anti-aggregation, and transfection activities into small biocompatible in vivo delivery conjugates. The use of reversible modification provides for physiologically responsive activity modulation.
    Type: Grant
    Filed: June 17, 2008
    Date of Patent: September 13, 2011
    Assignee: Roche Madison Inc.
    Inventors: Darren H. Wakefield, David B. Rozema, Sean D. Monahan, Lori Higgs, Jon A. Wolff, James E. Hagstrom
  • Endosomolytic poly(vinyl ether) polymers
    Patent number: 8008355
    Abstract: The present invention is directed to membrane active polymers useful for cellular delivery of compounds. Described are polyconjugates systems that incorporate targeting, anti-opsonization, anti-aggregation, and transfection activities into small biocompatible in vivo delivery conjugates. The use of reversible modification provides for physiologically responsive activity modulation.
    Type: Grant
    Filed: June 17, 2008
    Date of Patent: August 30, 2011
    Assignee: Roche Madison Inc.
    Inventors: David B. Rozema, Darren H. Wakefield, Sean D. Monahan, Lori Higgs, Jon A. Wolff, James E. Hagstrom
  • Membrane active heteropolymers
    Patent number: 7985406
    Abstract: The present invention is directed to a class of membrane active polymers useful for cellular delivery of compounds. Conjugation of the described membrane active polymers to targeting, anti-opsonization, and anti-aggregation agents provides polymers suitable for in vivo delivery. The use of multiple reversible linkages connecting component parts provides for physiologically responsive activity modulation.
    Type: Grant
    Filed: May 19, 2008
    Date of Patent: July 26, 2011
    Assignee: Roche Madison Inc.
    Inventors: Sean D. Monahan, Darren H. Wakefield, David B. Rozema, Lori Higgs, Jon A. Wolff, James E. Hagstrom
  • Ladder security bracket and safety system
    Patent number: 7909138
    Abstract: A system of increased safety when performing work using a ladder is described. The system includes a ladder bracket for temporarily attachment of a ladder to a structure to stabilize or secure the ladder. The bracket is easy to install and acts to limit or prevent slippage of the ladder laterally or away from the structure and generally promotes a greater margin of safety. In addition, the system includes a bracket for temporarily, yet securely, attaching a safety railing to a ladder.
    Type: Grant
    Filed: January 28, 2008
    Date of Patent: March 22, 2011
    Inventor: John R. Svehlek
  • Membrane active polymers
    Patent number: 7871818
    Abstract: We describe a class of polymaleic anhydride polymers capable of disrupting cell membranes. Co-delivery of these polymers with biologically active compounds increases cellular cytoplasmic delivery of the compounds.
    Type: Grant
    Filed: January 27, 2004
    Date of Patent: January 18, 2011
    Assignee: Roche Madison Inc.
    Inventors: David B. Rozema, Darren Wakefield
  • Intravenous delivery of polynucleotides to cells in mammalian limb
    Patent number: 7803782
    Abstract: An intravenous delivery method is described that enables delivery of polynucleotides to extravascular cells of a mammalian limb. The method involves the injection of polynucleotides into a distal vein of a limb that is transiently occluded. Polynucleotide delivery is facilitated by rapid injection in sufficient volume to enable extravasation of the solution into surrounding tissue.
    Type: Grant
    Filed: May 27, 2004
    Date of Patent: September 28, 2010
    Assignee: Roche Madison Inc.
    Inventors: James E. Hagstrom, Julia Hegge, Hans Herweijer, Jon A. Wolff
  • Process for delivering sirna to cardiac muscle tissue
    Patent number: 7781415
    Abstract: A process for delivering a polynucleotide to a cardiac tissue cell in a mammal is described, comprising introducing a composition consisting of a polynucleotide into a blood vessel and increasing permeability of the blood vessel to the polynucleotide. The polynucleotide can be a small interfering RNA or microRNA and inhibit gene expression in the cell.
    Type: Grant
    Filed: February 3, 2004
    Date of Patent: August 24, 2010
    Assignee: Roche Madison Inc.
    Inventors: Hans Herweijer, Julia Hegge, Jon A. Wolff, David L. Lewis
  • Polyvinylethers for delivery of polynucleotides to mammalian cells
    Patent number: 7682626
    Abstract: A class of polymers for delivery of polynucleotides to cells in described. More specifically, amphiphilic polyvinylethers and compositions containing amphiphilic polyvinylethers are described.
    Type: Grant
    Filed: February 5, 2004
    Date of Patent: March 23, 2010
    Assignee: Roche Madison Inc.
    Inventors: David B. Rozema, Darren Wakefield
  • Devices and processes for distribution of genetic material to mammalian limb
    Patent number: 7642248
    Abstract: A process is described for the delivery of a therapeutic polynucleotide to limb muscle tissue suffering from or potentially suffering from Muscular Dystrophy. The polynucleotide is inserted into a mammalian limb vessel such as an artery. Delivery efficiency and distribution is enhanced by combining injection of a solution containing the polynucleotide with the use of an externally applied cuff.
    Type: Grant
    Filed: March 30, 2007
    Date of Patent: January 5, 2010
    Assignee: Roche Madison Inc
    Inventors: Vladimir G. Budker, Jon A. Wolff
  • Delivery of molecules and complexes to mammalian cells in vivo
    Patent number: 7589059
    Abstract: Disclosed is a system for providing in vivo delivery of molecules or complexes to extravascular mammalian cells using an intravascular administration route. The molecules or complexes are inserted in an injection solution into a mammalian vasculature. Insertion of the injection solution at an appropriate rate transiently increases the volume of extravascular fluid in the tissue thereby facilitating delivery of the molecule to the cell.
    Type: Grant
    Filed: July 28, 2003
    Date of Patent: September 15, 2009
    Assignee: Roche Madison Inc.
    Inventors: Jon A. Wolff, Vladimir G. Budker, Hans Herweijer, James E. Hagstrom, Sean D. Monahan, Julia Hegge, Vladimir Subbotin
  • Nucleic acid injected into hepatic vein lumen
    Patent number: 7547683
    Abstract: Processes are described for obtaining high levels of gene expression in primates after injection of nucleic acid to the liver via the lumen of the hepatic vein. The described process results in high level of gene expression with transient increases in liver enzymes.
    Type: Grant
    Filed: October 10, 2006
    Date of Patent: June 16, 2009
    Assignee: Roche Madison, Inc.
    Inventors: Jon A. Wolff, Tatyana Budker, legal representative, Julia Hegge, James E. Hagstrom, Vladimir G. Budker
  • Polyampholytes for delivering polyions to a cell
    Patent number: 7524680
    Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Grant
    Filed: March 11, 2002
    Date of Patent: April 28, 2009
    Assignee: Roche Madison Inc.
    Inventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy
  • Compounds for targeting hepatocytes
    Patent number: 7501394
    Abstract: We describe compounds that bind to and are internalized by hepatocytes. Association of these compounds to other molecules or complexes can be used to target the molecules or complexes to hepatocytes in vivo or in vitro.
    Type: Grant
    Filed: January 20, 2006
    Date of Patent: March 10, 2009
    Assignee: Roche Madison Inc.
    Inventors: Alexander V. Sokoloff, So Wong, Jon A. Wolff, Sean D. Monahan, James Ludtke, Lori Higgs, Darren H. Wakefield, Magdolna G. Sebestyén
  • Compositions for delivering nucleic acids to cells
    Patent number: 7498017
    Abstract: Cyclic amidinium containing compounds and their methods of preparation are described. Compositions containing these compounds facilitate delivery of biologically active polymers to cells in vitro and in vivo.
    Type: Grant
    Filed: June 16, 2005
    Date of Patent: March 3, 2009
    Assignee: Roche Madison Inc.
    Inventors: Sean D. Monahan, Vladimir Trubetskoy
  • Process of making a compound by forming a polymer from a template drug
    Patent number: 7482160
    Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
    Type: Grant
    Filed: December 30, 2005
    Date of Patent: January 27, 2009
    Assignee: Roche Madison Inc.
    Inventors: Sean D. Monahan, David B. Rozema, Vladimir Trubetskoy, Paul M. Slattum, Jon A. Wolff, Vladimir G. Budker, James E. Hagstrom, Lisa J. Hanson