Abstract: Disclosed is a process for providing for expression of an exogenous nucleic acid in an extravascular parenchymal cell of a mammal. The nucleic acid is inserted into a vessel of a mammal and the permeability of the vessel is increased. Increasing permeability of the vessel allows delivery of the nucleic acid to an extravascular parenchymal cell.
Type:
Grant
Filed:
November 7, 2005
Date of Patent:
January 6, 2009
Assignee:
Roche Madison Inc.
Inventors:
Jon A. Wolff, James E. Hagstrom, Hans Herweijer, Vladimir G. Budker, Julia Hegge
Abstract: Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.
Type:
Grant
Filed:
October 21, 2005
Date of Patent:
December 30, 2008
Inventors:
Darren H. Wakefield, David B. Rozema, Jon A. Wolff, Vladimir Trubetskoy, James E. Hagstrom, Vladimir G. Budker, Jason Klein, So Wong
Abstract: An process for the reversible modification of an amine-containing compound is described. Modification of the compound can be used to facilitate delivery of molecules to cells in vitro and in vivo or to alter interactions or activities the compounds. The described modifiers can also be utilized as cross-linkers.
Type:
Grant
Filed:
December 20, 2005
Date of Patent:
October 28, 2008
Assignee:
Mirns Bio Corporation
Inventors:
David B. Rozema, Darren Wakefield, Jon A. Wolff, Kirk Ekena, James E. Hagstrom
Abstract: The present invention relates to compositions and methods for the selection and use of surface exposed epitopes. The present invention includes in vivo and in vitro phage peptide diplay methods for the identification and selection of peptides and peptide associated factors with desired properties (e.g., targeting specificity, stability, etc.). The present invention further provides methods and compositions for the isolation and identification of peptide-specific antibodies. The present invention also includes methods and compositions employing nuclear localization signals for enhanced nuclear transport and expression of DNA.
Abstract: Disclosed is a system for providing in vivo delivery of polynucleotides to mammalian prostate cells using an intravascular administration route. The polynucleotides are inserted in an injection solution into a mammalian vasculature. Insertion of the injection solution at an appropriate rate increases the volume of extravascular fluid in the tissue thereby facilitating delivery of the polynucleotide to the cell.
Type:
Grant
Filed:
October 6, 2003
Date of Patent:
October 14, 2008
Assignee:
Mirus Bio Corporation
Inventors:
James E. Hagstrom, Mark Noble, Julia Hegge, Vladimir G. Budker
Abstract: Compounds and methods are provided for a single-pot covalent attachment of a label to nucleic acids comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
Type:
Grant
Filed:
May 1, 2006
Date of Patent:
February 5, 2008
Assignee:
Mirus Bio Corporation
Inventors:
Paul M. Slattum, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker
Abstract: Described is a deliverable composition with low toxicity comprising an amphipathic compound, a polycation, and a siRNA. The composition may be used in the process of delivering a siRNA to an animal cell or more particularly, a mammal cell.
Type:
Grant
Filed:
May 28, 2002
Date of Patent:
September 5, 2006
Assignee:
Mirus Bio Corporation
Inventors:
David L. Lewis, James E. Hagstrom, Hans Herweijer, Aaron G. Loomis, Sean D. Monahan, Jon A. Wolff